CsaV3_5G031000 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_5G031000 vs. NCBI nr
Match: XP_004135278.1 (GDSL esterase/lipase At3g26430 [Cucumis sativus] >KGN51744.1 hypothetical protein Csa_009271 [Cucumis sativus])

HSP 1 Score: 812.8 bits (2098), Expect = 1.3e-231
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0

Query: 1   MESHSLIIAFTTFLALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60
           MESHSLIIAFTTFLALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR
Sbjct: 1   MESHSLIIAFTTFLALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60

Query: 61  SYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120
           SYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTL
Sbjct: 61  SYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120

Query: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180
           QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT
Sbjct: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180

Query: 181 SGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPA 240
           SGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPA
Sbjct: 181 SGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPA 240

Query: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300
           SDYDQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGF
Sbjct: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300

Query: 301 EYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360
           EYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW
Sbjct: 301 EYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360

Query: 361 IFNQIKDGSYSDPPIPLNKACHKT 385
           IFNQIKDGSYSDPPIPLNKACHKT
Sbjct: 361 IFNQIKDGSYSDPPIPLNKACHKT 384

BLAST of CsaV3_5G031000 vs. NCBI nr
Match: XP_008446147.1 (PREDICTED: GDSL esterase/lipase At3g26430 [Cucumis melo] >KAA0034264.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK15657.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 782.3 bits (2019), Expect = 1.9e-222
Identity = 371/384 (96.61%), Postives = 376/384 (97.92%), Query Frame = 0

Query: 1   MESHSLIIAFTTFLALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60
           MESHSLIIAFTTFLALSL P P+LAK PCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR
Sbjct: 1   MESHSLIIAFTTFLALSLIPCPSLAKHPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60

Query: 61  SYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120
           SYFPGPAGRYSDGRLIVDFIAES GLP+LSAYLDALGANFSHGANFATAGSTIRLQNRTL
Sbjct: 61  SYFPGPAGRYSDGRLIVDFIAESLGLPYLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120

Query: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180
           QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT
Sbjct: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180

Query: 181 SGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPA 240
           SGYFANM+LHQVKLY+PDVL QFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDRE VPA
Sbjct: 181 SGYFANMSLHQVKLYIPDVLQQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREPVPA 240

Query: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300
           SDYDQYGCATPFNDLAQYFNRGLKQAV+ELRKALPDSAITYVDVYSLKYALVSQHKKYGF
Sbjct: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVMELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300

Query: 301 EYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360
           EYPLRTCCGHGGKYNFNV LGCGGKK INGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW
Sbjct: 301 EYPLRTCCGHGGKYNFNVKLGCGGKKTINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360

Query: 361 IFNQIKDGSYSDPPIPLNKACHKT 385
           IFNQIKDGSYSDPPIPLNKACHKT
Sbjct: 361 IFNQIKDGSYSDPPIPLNKACHKT 384

BLAST of CsaV3_5G031000 vs. NCBI nr
Match: XP_038891379.1 (GDSL esterase/lipase At3g26430 [Benincasa hispida])

HSP 1 Score: 730.3 bits (1884), Expect = 8.5e-207
Identity = 344/385 (89.35%), Postives = 365/385 (94.81%), Query Frame = 0

Query: 1   MESHSLIIAFTTF--LALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPN 60
           ME HSLIIAFTTF  LALS+ P   LA++PCNFPAVFNFGDSNSDTGGLSAVFGQAPPPN
Sbjct: 18  MECHSLIIAFTTFIALALSVIPCSVLAREPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPN 77

Query: 61  GRSYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNR 120
           GRSYFPGPAGRYSDGRLIVDFIA+S GLP+LSAYLDALGANF+HGANFATAGSTIR QN 
Sbjct: 78  GRSYFPGPAGRYSDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTIRPQNT 137

Query: 121 TLQQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQND 180
           TLQQSGFSPISLNVQYNEFYDFRRRSQTLRN LGGIF+QLLPKEESFSRALYTFDIGQND
Sbjct: 138 TLQQSGFSPISLNVQYNEFYDFRRRSQTLRNRLGGIFEQLLPKEESFSRALYTFDIGQND 197

Query: 181 LTSGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHV 240
           LTSGYF NMT+ +VKLY+P+VLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDR+ V
Sbjct: 198 LTSGYFTNMTVDEVKLYIPEVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDRKPV 257

Query: 241 PASDYDQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKY 300
            ASDYDQYGCATPFNDLAQ FNRGLKQAV+ELRKALPD+AITYVD+YSLKYALV+QHKKY
Sbjct: 258 AASDYDQYGCATPFNDLAQSFNRGLKQAVLELRKALPDAAITYVDIYSLKYALVAQHKKY 317

Query: 301 GFEYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAAN 360
           GFE+PLRTCCGHGGKYNFN+ LGCGGKK+INGK+VLIGKSCKNPEVYVNWDGVHYTQAAN
Sbjct: 318 GFEFPLRTCCGHGGKYNFNLKLGCGGKKKINGKDVLIGKSCKNPEVYVNWDGVHYTQAAN 377

Query: 361 KWIFNQIKDGSYSDPPIPLNKACHK 384
           KWIFNQIKDG YSDPPIPL  ACH+
Sbjct: 378 KWIFNQIKDGFYSDPPIPLKMACHR 402

BLAST of CsaV3_5G031000 vs. NCBI nr
Match: XP_022966842.1 (GDSL esterase/lipase At3g26430 [Cucurbita maxima])

HSP 1 Score: 669.5 bits (1726), Expect = 1.8e-188
Identity = 315/383 (82.25%), Postives = 339/383 (88.51%), Query Frame = 0

Query: 1   MESHSLIIAFTTFLALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60
           ME HSL+IA     AL L P   LA+ PCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNG 
Sbjct: 1   MECHSLMIA---VFALLLVPHSVLARKPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGM 60

Query: 61  SYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120
           SYF GPAGRY DGRL++DFIA+S GLP+LSAYL+ALGANFSHGANFATAGST+R QNRTL
Sbjct: 61  SYFHGPAGRYCDGRLVIDFIAQSLGLPYLSAYLNALGANFSHGANFATAGSTVRPQNRTL 120

Query: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180
           QQSGFSPISL+VQYNEFYDFRRRSQT+RN LGG FK LLPKE SF +ALYTFDIGQNDLT
Sbjct: 121 QQSGFSPISLDVQYNEFYDFRRRSQTVRNRLGGFFKPLLPKETSFGQALYTFDIGQNDLT 180

Query: 181 SGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPA 240
           SGYFANMT+ QV+ Y+P VL QFS IVKWVHSQGGRFFWIHNTGPVGCLPYVLDR  V  
Sbjct: 181 SGYFANMTVEQVRDYIPQVLDQFSTIVKWVHSQGGRFFWIHNTGPVGCLPYVLDRMPVSP 240

Query: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300
           SDYDQYGCA PFNDLAQ FNRGLK AVVELR ALPD+A+TYVD+YSLKY LVSQHKKYGF
Sbjct: 241 SDYDQYGCAIPFNDLAQTFNRGLKDAVVELRAALPDAALTYVDIYSLKYDLVSQHKKYGF 300

Query: 301 EYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360
           E+PLRTCCGHGGKYNFNV LGC GKK +NGK+VLIGK+C+ PEVYVNWDGVHYTQAANKW
Sbjct: 301 EFPLRTCCGHGGKYNFNVKLGCAGKKRMNGKDVLIGKACEKPEVYVNWDGVHYTQAANKW 360

Query: 361 IFNQIKDGSYSDPPIPLNKACHK 384
           IFN IK G+YSDPPIPL  ACH+
Sbjct: 361 IFNHIKTGAYSDPPIPLEMACHR 380

BLAST of CsaV3_5G031000 vs. NCBI nr
Match: XP_023541593.1 (alpha-L-fucosidase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 668.7 bits (1724), Expect = 3.0e-188
Identity = 315/383 (82.25%), Postives = 340/383 (88.77%), Query Frame = 0

Query: 1   MESHSLIIAFTTFLALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60
           ME HSL+IA     AL L P   LA+ PCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNG 
Sbjct: 1   MECHSLMIA---VFALLLVPHSVLARKPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGM 60

Query: 61  SYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120
           SYF GPAGRY DGRL++DFIA+S GLP+LSAYL+ALGANFSHGANFATAGST+R QNRTL
Sbjct: 61  SYFHGPAGRYCDGRLVIDFIAQSLGLPYLSAYLNALGANFSHGANFATAGSTVRPQNRTL 120

Query: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180
           QQSGFSPISL+VQYNEFYDFRRRSQT+RN LGG FK LLPKE SF RALYTFDIGQNDLT
Sbjct: 121 QQSGFSPISLDVQYNEFYDFRRRSQTVRNRLGGFFKPLLPKETSFGRALYTFDIGQNDLT 180

Query: 181 SGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPA 240
           SGYFANMT+ QV+ Y+P VL QFS IVKWVHSQGGRFFWIHNTGPVGCLPYVLDR  V  
Sbjct: 181 SGYFANMTVKQVRDYIPQVLGQFSTIVKWVHSQGGRFFWIHNTGPVGCLPYVLDRMPVSP 240

Query: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300
           SDYDQYGCA PFNDLAQ FNRGLK AVV+LR ALPD+A+TYVD+YSLKY LVSQHKK+GF
Sbjct: 241 SDYDQYGCAIPFNDLAQTFNRGLKDAVVKLRAALPDAALTYVDIYSLKYDLVSQHKKHGF 300

Query: 301 EYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360
           E+PLRTCCGHGGKYNFNV LGC GKK +NGK+VLIGK+C+ PEVYVNWDGVHYTQAANKW
Sbjct: 301 EFPLRTCCGHGGKYNFNVKLGCAGKKRMNGKDVLIGKACEKPEVYVNWDGVHYTQAANKW 360

Query: 361 IFNQIKDGSYSDPPIPLNKACHK 384
           IFNQIK G+YSDPPIPL  ACH+
Sbjct: 361 IFNQIKTGAYSDPPIPLEMACHR 380

BLAST of CsaV3_5G031000 vs. ExPASy Swiss-Prot
Match: Q9LIN2 (GDSL esterase/lipase At3g26430 OS=Arabidopsis thaliana OX=3702 GN=At3g26430 PE=1 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 2.3e-138
Identity = 229/381 (60.10%), Postives = 290/381 (76.12%), Query Frame = 0

Query: 1   MESHSLIIAFTTFLALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60
           ME++ L++     L  S    P      CNFPA+FNFGDSNSDTGGLSA FGQAP PNG+
Sbjct: 1   METNLLLV--KCVLLASCLIHPRACSPSCNFPAIFNFGDSNSDTGGLSASFGQAPYPNGQ 60

Query: 61  SYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120
           ++F  P+GR+SDGRLI+DFIAE  GLP+L+A+LD++G+NFSHGANFATAGST+R  N T+
Sbjct: 61  TFFHSPSGRFSDGRLIIDFIAEELGLPYLNAFLDSIGSNFSHGANFATAGSTVRPPNATI 120

Query: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180
            QSG SPISL+VQ  +F DF  RSQ +RN  GG+FK+LLPK+E FS+ALYTFDIGQNDLT
Sbjct: 121 AQSGVSPISLDVQLVQFSDFITRSQLIRN-RGGVFKKLLPKKEYFSQALYTFDIGQNDLT 180

Query: 181 SGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPA 240
           +G   NMT  Q+K Y+PDV  Q S +++ V+S+GGR FWIHNT P+GCLPYVLDR  VPA
Sbjct: 181 AGLKLNMTSDQIKAYIPDVHDQLSNVIRKVYSKGGRRFWIHNTAPLGCLPYVLDRFPVPA 240

Query: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300
           S  D +GCA P N++A+Y+N  LK+ V+ELRK L ++A TYVD+YS+K  L++Q KK GF
Sbjct: 241 SQIDNHGCAIPRNEIARYYNSELKRRVIELRKELSEAAFTYVDIYSIKLTLITQAKKLGF 300

Query: 301 EYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360
            YPL  CCGHGGKYNFN  + CG K  I GKE+++ KSC +    V+WDG+H+T+  N W
Sbjct: 301 RYPLVACCGHGGKYNFNKLIKCGAKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETTNSW 360

Query: 361 IFNQIKDGSYSDPPIPLNKAC 382
           IF QI DG++SDPP+P+  AC
Sbjct: 361 IFQQINDGAFSDPPLPVKSAC 378

BLAST of CsaV3_5G031000 vs. ExPASy Swiss-Prot
Match: B4FZ87 (GDSL esterase/lipase ACHE OS=Zea mays OX=4577 GN=ACHE PE=1 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 1.7e-133
Identity = 221/379 (58.31%), Postives = 292/379 (77.04%), Query Frame = 0

Query: 5   SLIIAFTTFLALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGRSYFP 64
           ++++  +  LAL+L P  A A   C+FPAVFNFGDSNSDTGGLS++FG APPPNGR++F 
Sbjct: 14  AVLLLLSLALALALRPSDAGAGGDCHFPAVFNFGDSNSDTGGLSSLFGAAPPPNGRTFFG 73

Query: 65  GPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTLQQSG 124
            PAGRY DGRL++DFIAES GL HLSAYL+++G+NF+ GANFATAGS+IR QN +L  SG
Sbjct: 74  MPAGRYCDGRLVIDFIAESLGLTHLSAYLNSIGSNFTQGANFATAGSSIRRQNTSLFLSG 133

Query: 125 FSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLTSGYF 184
           FSPISL+VQ+ EF  F  RSQ + N  GGI++++LP+ E FS+ALYTFDIGQND+TS YF
Sbjct: 134 FSPISLDVQFWEFEQFINRSQLVYNNKGGIYREILPRAEYFSQALYTFDIGQNDITSSYF 193

Query: 185 ANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPASDYD 244
            N T  +V+  +PD++ + + I++ V+S+GGR+FWIHNTGP+GCLPY L      A   D
Sbjct: 194 VNNTTEEVEAIIPDLMERLTSIIQSVYSRGGRYFWIHNTGPLGCLPYALLHRPDLAIPAD 253

Query: 245 QYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGFEYPL 304
             GC+  +N +AQ FN  LK+ V  LRK  PD+A TYVDVY+ KY L+SQ  K GF+ PL
Sbjct: 254 GTGCSVTYNKVAQLFNLRLKETVASLRKTHPDAAFTYVDVYTAKYKLISQANKLGFDDPL 313

Query: 305 RTCCGH-GGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKWIFN 364
            TCCG+ GG+YN ++++GCGGKK++NG  V++GKSC+NP   V+WDGVH+T+AANK++F+
Sbjct: 314 LTCCGYGGGRYNLDLSVGCGGKKQVNGTSVVVGKSCENPSKRVSWDGVHFTEAANKFVFD 373

Query: 365 QIKDGSYSDPPIPLNKACH 383
           QI  G+ SDPP+ L +ACH
Sbjct: 374 QIVAGALSDPPVALRQACH 392

BLAST of CsaV3_5G031000 vs. ExPASy Swiss-Prot
Match: Q9FXE5 (Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 3.8e-133
Identity = 222/362 (61.33%), Postives = 282/362 (77.90%), Query Frame = 0

Query: 22  PALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGRSYFPGPAGRYSDGRLIVDFIA 81
           P+     C+FPA+FNFGDSNSDTGGLSA FGQA PP+G S+F  PAGRY DGRL++DFIA
Sbjct: 19  PSTHAHQCHFPAIFNFGDSNSDTGGLSAAFGQAGPPHGSSFFGSPAGRYCDGRLVIDFIA 78

Query: 82  ESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTLQQSGFSPISLNVQYNEFYDFR 141
           ES GLP+LSA+LD++G+NFSHGANFATAGS IR  N TL+QSGFSP SL+VQ+ +FY+F 
Sbjct: 79  ESLGLPYLSAFLDSVGSNFSHGANFATAGSPIRALNSTLRQSGFSPFSLDVQFVQFYNFH 138

Query: 142 RRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLTSGYFANMTLHQVKLYVPDVLH 201
            RSQT+R+  GG++K +LP+ +SFS+ALYTFDIGQNDLT+GYFAN T+ QV+  VP+++ 
Sbjct: 139 NRSQTVRS-RGGVYKTMLPESDSFSKALYTFDIGQNDLTAGYFANKTVEQVETEVPEIIS 198

Query: 202 QFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPASDYDQYGCATPFNDLAQYFNR 261
           QF   +K ++ QGGR+FWIHNTGP+GCL YV++R    ASD+D +GC +P N LAQ FN 
Sbjct: 199 QFMNAIKNIYGQGGRYFWIHNTGPIGCLAYVIERFPNKASDFDSHGCVSPLNHLAQQFNH 258

Query: 262 GLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGFEYPLRTCCGHGGKYNFNVNLG 321
            LKQAV+ELR +L ++AITYVDVYSLK+ L    + +GF+  L +CCGHGGKYN+N  +G
Sbjct: 259 ALKQAVIELRSSLSEAAITYVDVYSLKHELFVHAQGHGFKGSLVSCCGHGGKYNYNKGIG 318

Query: 322 CGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKWIFNQIKDGSYSDPPIPLNKAC 381
           CG KK + GKEV IGK C  P+  V WDGVH+TQAANK+IF++I  G        L+KAC
Sbjct: 319 CGMKKIVKGKEVYIGKPCDEPDKAVVWDGVHFTQAANKFIFDKIAPG--------LSKAC 371

Query: 382 HK 384
            +
Sbjct: 379 KR 371

BLAST of CsaV3_5G031000 vs. ExPASy Swiss-Prot
Match: Q7Y1X1 (Esterase OS=Hevea brasiliensis OX=3981 PE=1 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 4.2e-116
Identity = 201/364 (55.22%), Postives = 265/364 (72.80%), Query Frame = 0

Query: 23  ALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGRSYFPGPAGRYSDGRLIVDFIAE 82
           A A + C+FPA+FNFGDSNSDTGG +A F    PP G ++F    GRYSDGRLI+DFIAE
Sbjct: 24  AYASETCDFPAIFNFGDSNSDTGGKAAAFYPLNPPYGETFFHRSTGRYSDGRLIIDFIAE 83

Query: 83  SFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTL-QQSGFSPISLNVQYNEFYDFR 142
           SF LP+LS YL +LG+NF HGA+FATAGSTI+L    +    GFSP  L+VQY++F  F 
Sbjct: 84  SFNLPYLSPYLSSLGSNFKHGADFATAGSTIKLPTTIIPAHGGFSPFYLDVQYSQFRQFI 143

Query: 143 RRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLTSGYFANMTLHQVKLYVPDVLH 202
            RSQ +R   GGIF +L+P+E  F +ALYTFDIGQNDLT G F N+T+ +V   VPD+++
Sbjct: 144 PRSQFIRE-TGGIFAELVPEEYYFEKALYTFDIGQNDLTEG-FLNLTVEEVNATVPDLVN 203

Query: 203 QFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPASDYDQYGCATPFNDLAQYFNR 262
            FS  VK ++  G R FWIHNTGP+GCL ++L   + P ++ D  GCA  +N++AQ+FN 
Sbjct: 204 SFSANVKKIYDLGARTFWIHNTGPIGCLSFIL--TYFPWAEKDSAGCAKAYNEVAQHFNH 263

Query: 263 GLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGFEYPLRTCCGHGGKYNFNVNLG 322
            LK+ V +LRK LP +   +VD+YS+KY+L S+ +K+GFE+PL TCCG+GGKYNF+V   
Sbjct: 264 KLKEIVAQLRKDLPLATFVHVDIYSVKYSLFSEPEKHGFEFPLITCCGYGGKYNFSVTAP 323

Query: 323 CGGKKEI-NGKEVLIGKSCKNPEVYVNWDGVHYTQAANKWIFNQIKDGSYSDPPIPLNKA 382
           CG      +G ++++G SC  P V VNWDG HYT+AAN++ F+QI  G++SDPP+PLN A
Sbjct: 324 CGDTVTADDGTKIVVG-SCACPSVRVNWDGAHYTEAANEYFFDQISTGAFSDPPVPLNMA 382

Query: 383 CHKT 385
           CHKT
Sbjct: 384 CHKT 382

BLAST of CsaV3_5G031000 vs. ExPASy Swiss-Prot
Match: Q9LY84 (GDSL esterase/lipase At5g14450 OS=Arabidopsis thaliana OX=3702 GN=At5g14450 PE=2 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 8.2e-104
Identity = 187/389 (48.07%), Postives = 259/389 (66.58%), Query Frame = 0

Query: 1   MESHSLIIAFTTF----LALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPP 60
           +E   L+++ T F    L L         +  C FPA++NFGDSNSDTGG+SA F     
Sbjct: 5   LERAKLMVSSTVFSWLLLCLFAVTTSVSVQPTCTFPAIYNFGDSNSDTGGISAAFEPIRD 64

Query: 61  PNGRSYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQ 120
           P G+ +F  P GR SDGRL +DFIAE  GLP+LSAYL++LG+NF HGANFAT GSTIR Q
Sbjct: 65  PYGQGFFHRPTGRDSDGRLTIDFIAERLGLPYLSAYLNSLGSNFRHGANFATGGSTIRRQ 124

Query: 121 NRTLQQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIF-KQLLPKEESFSRALYTFDIG 180
           N T+ Q G SP SL++Q  +F  F+ RS  L   +   + ++ LP++E F++ALYTFDIG
Sbjct: 125 NETIFQYGISPFSLDMQIAQFDQFKARSALLFTQIKSRYDREKLPRQEEFAKALYTFDIG 184

Query: 181 QNDLTSGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDR 240
           QNDL+ G F  M++ Q+K  +PD+++  +  V+ ++ QGGR FW+HNTGP GCLP  +  
Sbjct: 185 QNDLSVG-FRTMSVDQLKATIPDIVNHLASAVRNIYQQGGRTFWVHNTGPFGCLPVNMFY 244

Query: 241 EHVPASDY-DQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQ 300
              PA  Y D+ GC    N++A  FNR LK+ V+ LRK L  +AITYVDVY+ KY ++S 
Sbjct: 245 MGTPAPGYLDKSGCVKAQNEMAMEFNRKLKETVINLRKELTQAAITYVDVYTAKYEMMSN 304

Query: 301 HKKYGFEYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYT 360
            KK GF  PL+ CCG+  KY+   ++ CG K ++N  E+  G SC NP + V+WDGVHYT
Sbjct: 305 PKKLGFANPLKVCCGYHEKYD---HIWCGNKGKVNNTEI-YGGSCPNPVMAVSWDGVHYT 364

Query: 361 QAANKWIFNQIKDGSYSDPPIPLNKACHK 384
           +AANK + ++  +G  +DPP+P+ +AC++
Sbjct: 365 EAANKHVADRTLNGLLTDPPVPITRACYR 388

BLAST of CsaV3_5G031000 vs. ExPASy TrEMBL
Match: A0A0A0KS60 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G598030 PE=3 SV=1)

HSP 1 Score: 812.8 bits (2098), Expect = 6.3e-232
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0

Query: 1   MESHSLIIAFTTFLALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60
           MESHSLIIAFTTFLALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR
Sbjct: 1   MESHSLIIAFTTFLALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60

Query: 61  SYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120
           SYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTL
Sbjct: 61  SYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120

Query: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180
           QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT
Sbjct: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180

Query: 181 SGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPA 240
           SGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPA
Sbjct: 181 SGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPA 240

Query: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300
           SDYDQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGF
Sbjct: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300

Query: 301 EYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360
           EYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW
Sbjct: 301 EYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360

Query: 361 IFNQIKDGSYSDPPIPLNKACHKT 385
           IFNQIKDGSYSDPPIPLNKACHKT
Sbjct: 361 IFNQIKDGSYSDPPIPLNKACHKT 384

BLAST of CsaV3_5G031000 vs. ExPASy TrEMBL
Match: A0A5A7SSS5 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001540 PE=3 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 9.1e-223
Identity = 371/384 (96.61%), Postives = 376/384 (97.92%), Query Frame = 0

Query: 1   MESHSLIIAFTTFLALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60
           MESHSLIIAFTTFLALSL P P+LAK PCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR
Sbjct: 1   MESHSLIIAFTTFLALSLIPCPSLAKHPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60

Query: 61  SYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120
           SYFPGPAGRYSDGRLIVDFIAES GLP+LSAYLDALGANFSHGANFATAGSTIRLQNRTL
Sbjct: 61  SYFPGPAGRYSDGRLIVDFIAESLGLPYLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120

Query: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180
           QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT
Sbjct: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180

Query: 181 SGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPA 240
           SGYFANM+LHQVKLY+PDVL QFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDRE VPA
Sbjct: 181 SGYFANMSLHQVKLYIPDVLQQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREPVPA 240

Query: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300
           SDYDQYGCATPFNDLAQYFNRGLKQAV+ELRKALPDSAITYVDVYSLKYALVSQHKKYGF
Sbjct: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVMELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300

Query: 301 EYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360
           EYPLRTCCGHGGKYNFNV LGCGGKK INGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW
Sbjct: 301 EYPLRTCCGHGGKYNFNVKLGCGGKKTINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360

Query: 361 IFNQIKDGSYSDPPIPLNKACHKT 385
           IFNQIKDGSYSDPPIPLNKACHKT
Sbjct: 361 IFNQIKDGSYSDPPIPLNKACHKT 384

BLAST of CsaV3_5G031000 vs. ExPASy TrEMBL
Match: A0A1S3BF19 (GDSL esterase/lipase At3g26430 OS=Cucumis melo OX=3656 GN=LOC103488959 PE=3 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 9.1e-223
Identity = 371/384 (96.61%), Postives = 376/384 (97.92%), Query Frame = 0

Query: 1   MESHSLIIAFTTFLALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60
           MESHSLIIAFTTFLALSL P P+LAK PCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR
Sbjct: 1   MESHSLIIAFTTFLALSLIPCPSLAKHPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60

Query: 61  SYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120
           SYFPGPAGRYSDGRLIVDFIAES GLP+LSAYLDALGANFSHGANFATAGSTIRLQNRTL
Sbjct: 61  SYFPGPAGRYSDGRLIVDFIAESLGLPYLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120

Query: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180
           QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT
Sbjct: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180

Query: 181 SGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPA 240
           SGYFANM+LHQVKLY+PDVL QFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDRE VPA
Sbjct: 181 SGYFANMSLHQVKLYIPDVLQQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREPVPA 240

Query: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300
           SDYDQYGCATPFNDLAQYFNRGLKQAV+ELRKALPDSAITYVDVYSLKYALVSQHKKYGF
Sbjct: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVMELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300

Query: 301 EYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360
           EYPLRTCCGHGGKYNFNV LGCGGKK INGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW
Sbjct: 301 EYPLRTCCGHGGKYNFNVKLGCGGKKTINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360

Query: 361 IFNQIKDGSYSDPPIPLNKACHKT 385
           IFNQIKDGSYSDPPIPLNKACHKT
Sbjct: 361 IFNQIKDGSYSDPPIPLNKACHKT 384

BLAST of CsaV3_5G031000 vs. ExPASy TrEMBL
Match: A0A6J1HP45 (GDSL esterase/lipase At3g26430 OS=Cucurbita maxima OX=3661 GN=LOC111466427 PE=3 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 8.6e-189
Identity = 315/383 (82.25%), Postives = 339/383 (88.51%), Query Frame = 0

Query: 1   MESHSLIIAFTTFLALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60
           ME HSL+IA     AL L P   LA+ PCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNG 
Sbjct: 1   MECHSLMIA---VFALLLVPHSVLARKPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGM 60

Query: 61  SYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120
           SYF GPAGRY DGRL++DFIA+S GLP+LSAYL+ALGANFSHGANFATAGST+R QNRTL
Sbjct: 61  SYFHGPAGRYCDGRLVIDFIAQSLGLPYLSAYLNALGANFSHGANFATAGSTVRPQNRTL 120

Query: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180
           QQSGFSPISL+VQYNEFYDFRRRSQT+RN LGG FK LLPKE SF +ALYTFDIGQNDLT
Sbjct: 121 QQSGFSPISLDVQYNEFYDFRRRSQTVRNRLGGFFKPLLPKETSFGQALYTFDIGQNDLT 180

Query: 181 SGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPA 240
           SGYFANMT+ QV+ Y+P VL QFS IVKWVHSQGGRFFWIHNTGPVGCLPYVLDR  V  
Sbjct: 181 SGYFANMTVEQVRDYIPQVLDQFSTIVKWVHSQGGRFFWIHNTGPVGCLPYVLDRMPVSP 240

Query: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300
           SDYDQYGCA PFNDLAQ FNRGLK AVVELR ALPD+A+TYVD+YSLKY LVSQHKKYGF
Sbjct: 241 SDYDQYGCAIPFNDLAQTFNRGLKDAVVELRAALPDAALTYVDIYSLKYDLVSQHKKYGF 300

Query: 301 EYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360
           E+PLRTCCGHGGKYNFNV LGC GKK +NGK+VLIGK+C+ PEVYVNWDGVHYTQAANKW
Sbjct: 301 EFPLRTCCGHGGKYNFNVKLGCAGKKRMNGKDVLIGKACEKPEVYVNWDGVHYTQAANKW 360

Query: 361 IFNQIKDGSYSDPPIPLNKACHK 384
           IFN IK G+YSDPPIPL  ACH+
Sbjct: 361 IFNHIKTGAYSDPPIPLEMACHR 380

BLAST of CsaV3_5G031000 vs. ExPASy TrEMBL
Match: A0A6J1G253 (alpha-L-fucosidase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111449990 PE=3 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 5.2e-186
Identity = 311/383 (81.20%), Postives = 337/383 (87.99%), Query Frame = 0

Query: 1   MESHSLIIAFTTFLALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60
           ME  SL+IA     AL L P   LA+ PCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNG 
Sbjct: 1   MECRSLMIA---VFALLLVPHSVLARKPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGM 60

Query: 61  SYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120
           SYF GPAGRY DGRL++DFIAES GLP+LSAYL+ALGANFSHGANFATAGST+R QNRTL
Sbjct: 61  SYFHGPAGRYCDGRLVIDFIAESLGLPYLSAYLNALGANFSHGANFATAGSTVRPQNRTL 120

Query: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180
           QQSGFSPISL+VQYNEFYDFRRRSQT+RN LGG FK LLPKE SF RALYTFDIGQNDLT
Sbjct: 121 QQSGFSPISLDVQYNEFYDFRRRSQTVRNRLGGFFKPLLPKETSFGRALYTFDIGQNDLT 180

Query: 181 SGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPA 240
           SGYFANMT+ QV+ Y+P VL+QFS IVKWVHSQGGRFFWIHNTGPVGCLPYVLDR  V  
Sbjct: 181 SGYFANMTVEQVRDYIPQVLNQFSTIVKWVHSQGGRFFWIHNTGPVGCLPYVLDRMPVSP 240

Query: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300
           SDYDQYGCA PFNDLAQ FN GLK AVV+LR ALP++ +TYVD+YSLKY LVSQHKK+GF
Sbjct: 241 SDYDQYGCAIPFNDLAQTFNHGLKDAVVKLRAALPNATLTYVDIYSLKYDLVSQHKKHGF 300

Query: 301 EYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360
           E+PLRTCCGHGGKYNFNV LGC GKK +NGK+VLIGK+CK PEVYVNWDGVHYTQAAN W
Sbjct: 301 EFPLRTCCGHGGKYNFNVKLGCAGKKRMNGKDVLIGKACKKPEVYVNWDGVHYTQAANNW 360

Query: 361 IFNQIKDGSYSDPPIPLNKACHK 384
           IFNQIK G+YSDPPIPL  AC++
Sbjct: 361 IFNQIKTGAYSDPPIPLEMACYR 380

BLAST of CsaV3_5G031000 vs. TAIR 10
Match: AT3G26430.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 493.4 bits (1269), Expect = 1.6e-139
Identity = 229/381 (60.10%), Postives = 290/381 (76.12%), Query Frame = 0

Query: 1   MESHSLIIAFTTFLALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60
           ME++ L++     L  S    P      CNFPA+FNFGDSNSDTGGLSA FGQAP PNG+
Sbjct: 1   METNLLLV--KCVLLASCLIHPRACSPSCNFPAIFNFGDSNSDTGGLSASFGQAPYPNGQ 60

Query: 61  SYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120
           ++F  P+GR+SDGRLI+DFIAE  GLP+L+A+LD++G+NFSHGANFATAGST+R  N T+
Sbjct: 61  TFFHSPSGRFSDGRLIIDFIAEELGLPYLNAFLDSIGSNFSHGANFATAGSTVRPPNATI 120

Query: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180
            QSG SPISL+VQ  +F DF  RSQ +RN  GG+FK+LLPK+E FS+ALYTFDIGQNDLT
Sbjct: 121 AQSGVSPISLDVQLVQFSDFITRSQLIRN-RGGVFKKLLPKKEYFSQALYTFDIGQNDLT 180

Query: 181 SGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPA 240
           +G   NMT  Q+K Y+PDV  Q S +++ V+S+GGR FWIHNT P+GCLPYVLDR  VPA
Sbjct: 181 AGLKLNMTSDQIKAYIPDVHDQLSNVIRKVYSKGGRRFWIHNTAPLGCLPYVLDRFPVPA 240

Query: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300
           S  D +GCA P N++A+Y+N  LK+ V+ELRK L ++A TYVD+YS+K  L++Q KK GF
Sbjct: 241 SQIDNHGCAIPRNEIARYYNSELKRRVIELRKELSEAAFTYVDIYSIKLTLITQAKKLGF 300

Query: 301 EYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360
            YPL  CCGHGGKYNFN  + CG K  I GKE+++ KSC +    V+WDG+H+T+  N W
Sbjct: 301 RYPLVACCGHGGKYNFNKLIKCGAKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETTNSW 360

Query: 361 IFNQIKDGSYSDPPIPLNKAC 382
           IF QI DG++SDPP+P+  AC
Sbjct: 361 IFQQINDGAFSDPPLPVKSAC 378

BLAST of CsaV3_5G031000 vs. TAIR 10
Match: AT1G67830.1 (alpha-fucosidase 1 )

HSP 1 Score: 476.1 bits (1224), Expect = 2.7e-134
Identity = 222/362 (61.33%), Postives = 282/362 (77.90%), Query Frame = 0

Query: 22  PALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGRSYFPGPAGRYSDGRLIVDFIA 81
           P+     C+FPA+FNFGDSNSDTGGLSA FGQA PP+G S+F  PAGRY DGRL++DFIA
Sbjct: 19  PSTHAHQCHFPAIFNFGDSNSDTGGLSAAFGQAGPPHGSSFFGSPAGRYCDGRLVIDFIA 78

Query: 82  ESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTLQQSGFSPISLNVQYNEFYDFR 141
           ES GLP+LSA+LD++G+NFSHGANFATAGS IR  N TL+QSGFSP SL+VQ+ +FY+F 
Sbjct: 79  ESLGLPYLSAFLDSVGSNFSHGANFATAGSPIRALNSTLRQSGFSPFSLDVQFVQFYNFH 138

Query: 142 RRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLTSGYFANMTLHQVKLYVPDVLH 201
            RSQT+R+  GG++K +LP+ +SFS+ALYTFDIGQNDLT+GYFAN T+ QV+  VP+++ 
Sbjct: 139 NRSQTVRS-RGGVYKTMLPESDSFSKALYTFDIGQNDLTAGYFANKTVEQVETEVPEIIS 198

Query: 202 QFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPASDYDQYGCATPFNDLAQYFNR 261
           QF   +K ++ QGGR+FWIHNTGP+GCL YV++R    ASD+D +GC +P N LAQ FN 
Sbjct: 199 QFMNAIKNIYGQGGRYFWIHNTGPIGCLAYVIERFPNKASDFDSHGCVSPLNHLAQQFNH 258

Query: 262 GLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGFEYPLRTCCGHGGKYNFNVNLG 321
            LKQAV+ELR +L ++AITYVDVYSLK+ L    + +GF+  L +CCGHGGKYN+N  +G
Sbjct: 259 ALKQAVIELRSSLSEAAITYVDVYSLKHELFVHAQGHGFKGSLVSCCGHGGKYNYNKGIG 318

Query: 322 CGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKWIFNQIKDGSYSDPPIPLNKAC 381
           CG KK + GKEV IGK C  P+  V WDGVH+TQAANK+IF++I  G        L+KAC
Sbjct: 319 CGMKKIVKGKEVYIGKPCDEPDKAVVWDGVHFTQAANKFIFDKIAPG--------LSKAC 371

Query: 382 HK 384
            +
Sbjct: 379 KR 371

BLAST of CsaV3_5G031000 vs. TAIR 10
Match: AT5G14450.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 378.6 bits (971), Expect = 5.8e-105
Identity = 187/389 (48.07%), Postives = 259/389 (66.58%), Query Frame = 0

Query: 1   MESHSLIIAFTTF----LALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPP 60
           +E   L+++ T F    L L         +  C FPA++NFGDSNSDTGG+SA F     
Sbjct: 5   LERAKLMVSSTVFSWLLLCLFAVTTSVSVQPTCTFPAIYNFGDSNSDTGGISAAFEPIRD 64

Query: 61  PNGRSYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQ 120
           P G+ +F  P GR SDGRL +DFIAE  GLP+LSAYL++LG+NF HGANFAT GSTIR Q
Sbjct: 65  PYGQGFFHRPTGRDSDGRLTIDFIAERLGLPYLSAYLNSLGSNFRHGANFATGGSTIRRQ 124

Query: 121 NRTLQQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIF-KQLLPKEESFSRALYTFDIG 180
           N T+ Q G SP SL++Q  +F  F+ RS  L   +   + ++ LP++E F++ALYTFDIG
Sbjct: 125 NETIFQYGISPFSLDMQIAQFDQFKARSALLFTQIKSRYDREKLPRQEEFAKALYTFDIG 184

Query: 181 QNDLTSGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDR 240
           QNDL+ G F  M++ Q+K  +PD+++  +  V+ ++ QGGR FW+HNTGP GCLP  +  
Sbjct: 185 QNDLSVG-FRTMSVDQLKATIPDIVNHLASAVRNIYQQGGRTFWVHNTGPFGCLPVNMFY 244

Query: 241 EHVPASDY-DQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQ 300
              PA  Y D+ GC    N++A  FNR LK+ V+ LRK L  +AITYVDVY+ KY ++S 
Sbjct: 245 MGTPAPGYLDKSGCVKAQNEMAMEFNRKLKETVINLRKELTQAAITYVDVYTAKYEMMSN 304

Query: 301 HKKYGFEYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYT 360
            KK GF  PL+ CCG+  KY+   ++ CG K ++N  E+  G SC NP + V+WDGVHYT
Sbjct: 305 PKKLGFANPLKVCCGYHEKYD---HIWCGNKGKVNNTEI-YGGSCPNPVMAVSWDGVHYT 364

Query: 361 QAANKWIFNQIKDGSYSDPPIPLNKACHK 384
           +AANK + ++  +G  +DPP+P+ +AC++
Sbjct: 365 EAANKHVADRTLNGLLTDPPVPITRACYR 388

BLAST of CsaV3_5G031000 vs. TAIR 10
Match: AT3G27950.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 342.8 bits (878), Expect = 3.5e-94
Identity = 181/372 (48.66%), Postives = 235/372 (63.17%), Query Frame = 0

Query: 14  LALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGRSYFPGPAGRYSDG 73
           L L  T   +     CNFPAVFNFGDSNSDTG +SA  G+ PPPNG ++F   AGR+SDG
Sbjct: 13  LLLGFTEKLSALSSSCNFPAVFNFGDSNSDTGAISAAIGEVPPPNGVAFFGRSAGRHSDG 72

Query: 74  RLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTLQQSGFSPISLNVQ 133
           RLI+DFI E+  LP+L+ YLD++GAN+ HGANFAT GS IR    TL  + FSP  L  Q
Sbjct: 73  RLIIDFITENLTLPYLTPYLDSVGANYRHGANFATGGSCIR---PTL--ACFSPFHLGTQ 132

Query: 134 YNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLTSGYFANMTLHQVK 193
            ++F  F+ R+ +L N           +   FS+ALYT DIGQNDL  G F NMT  Q+K
Sbjct: 133 VSQFIHFKTRTLSLYN-----------QTNDFSKALYTLDIGQNDLAIG-FQNMTEEQLK 192

Query: 194 LYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPASDYDQYGCATPFN 253
             +P ++  F+  +K ++ +G RFF IHNTGP GCLPY+L  +  PA   D YGC  P N
Sbjct: 193 ATIPLIIENFTIALKLLYKEGARFFSIHNTGPTGCLPYLL--KAFPAIPRDPYGCLKPLN 252

Query: 254 DLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGFEYPLRTCC-GHGG 313
           ++A  FN+ LK  + +L+K LP S  TYVDVYS KY L+++ K  GF  P   CC G  G
Sbjct: 253 NVAIEFNKQLKNKITQLKKELPSSFFTYVDVYSAKYNLITKAKALGFIDPFDYCCVGAIG 312

Query: 314 KYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKWIFNQIKDGSYSD 373
           +      +GCG    +NG E L   SC+N + +++WDG+HYT+ AN  + N+I DGS SD
Sbjct: 313 R-----GMGCGKTIFLNGTE-LYSSSCQNRKNFISWDGIHYTETANMLVANRILDGSISD 359

Query: 374 PPIPLNKACHKT 385
           PP+P  KAC  T
Sbjct: 373 PPLPTQKACKLT 359

BLAST of CsaV3_5G031000 vs. TAIR 10
Match: AT4G01130.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 305.8 bits (782), Expect = 4.8e-83
Identity = 160/358 (44.69%), Postives = 218/358 (60.89%), Query Frame = 0

Query: 29  CNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGRSYFPGPAGRYSDGRLIVDFIAESFGLPH 88
           C+F A+FNFGDSNSDTGG  A F     P G +YF  PAGR SDGRLI+DF+A+S G+P 
Sbjct: 30  CDFEAIFNFGDSNSDTGGFWAAFPAQSGPWGMTYFKKPAGRASDGRLIIDFLAKSLGMPF 89

Query: 89  LSAYLDALGANFSHGANFATAGSTIRLQNRTLQQSGFSPISLNVQYNEFYDFR---RRSQ 148
           LS YL ++G++F HGANFAT  ST+ L N +L  SG SP SL +Q N+   F+     S 
Sbjct: 90  LSPYLQSIGSDFRHGANFATLASTVLLPNTSLFVSGISPFSLAIQLNQMKQFKVNVDESH 149

Query: 149 TL-RNGLGGIFKQLLPKEESFSRALYTFDIGQNDLTSGYFANMTLHQVKLYVPDVLHQFS 208
           +L R GL     ++LP +  F ++LYTF IGQND TS   A++ + +VKLY+P V+ Q +
Sbjct: 150 SLDRPGL-----KILPSKIVFGKSLYTFYIGQNDFTSN-LASIGVERVKLYLPQVIGQIA 209

Query: 209 EIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPASDYDQYGCATPFNDLAQYFNRGLK 268
             +K ++  GGR F + N  PVGC P +L       +D D+YGC  P N   +Y+N  L 
Sbjct: 210 GTIKEIYGIGGRTFLVLNLAPVGCYPAILTGYTHTDADLDKYGCLIPVNKAVKYYNTLLN 269

Query: 269 QAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGFEYPLRTCCGHGGK-YNFNVNLGCG 328
           + + + R  L ++ + Y+D + +   L    K YG ++ ++ CCG+GG+ YNFN  L CG
Sbjct: 270 KTLSQTRTELKNATVIYLDTHKILLDLFQHPKSYGMKHGIKACCGYGGRPYNFNQKLFCG 329

Query: 329 GKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKWIFNQIKDGSYSDPPIPLNKAC 382
             K I G      K+C +P  YV+WDG+H T+AAN  I   I DGS S PP  LN  C
Sbjct: 330 NTKVI-GNFSTTAKACHDPHNYVSWDGIHATEAANHHISMAILDGSISYPPFILNNLC 380