CsaV3_5G030200 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_5G030200
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description4-coumarate--CoA ligase-like 1
Locationchr5: 24830798 .. 24833762 (+)
RNA-Seq ExpressionCsaV3_5G030200
SyntenyCsaV3_5G030200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAACATTGTTGAAAGAACTCGTGTTAGTTTTTGTGGTTGATTTTCTCTTTAGTATGGCTTAAGACTAACTAGAATGTCGTTTTTTGATTTAAGAGAATATAGGAGAATGACAAGATCAACTTGAGATATTATGGAGGGCTTCGTTTTTTCAAATAATTTTATGATGGATGCTGCTTCCAACTATAATCAGTAAACAAACCACAGAGAAAAACATGTTATGAATAATATAATTCTATATGGTTCAATTAATTTTAAAGCCAAGCTAGCTTCTGTTTAGAAAGAGAGCTTATGACTGCCTGAATGAATGGCATGAATCAACAATATCCCACCAAAAAAAAGGGCTCATGCCAATCTCACCCTCAAGAACTCTACAAGTTTCTCATTCACAAAGCATGATACAACCTTTCTCAACTCTTCTTTTAAGTAGAGGGAAGTTGACGCCTGCAGAAAACTACTATTCTGCCATGGCTACTTGCAACCAAGATTCGGTACAAGACGAGGAACACATTTTCTGCAGCCAACTTCCTGAGGTCCAAGTGCCTTATGATATCACACTGCCTGAGTTTGTACTCCAGAATGCTGAATCATATGCCGAGAATGTGGCATTTGTGGAAGCCATGAGCGGAAAGGCATACACTTACCGTGAAGTTTTAAGAGACACAAATAGATTTTCCAAGGCCTTGCGCTCTCTGAGGTTGAAGAAAGGACATGTGGTTATTGTTGTTCTACCTAATGTTGCTGAATACGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCAGGTGTGAATCCGGCAGCTCACATATCAGAAATCAAAAAGCAAGTGGAGGTAGCAGAAGCAAAACTTGTCGTCACAAATGCCGCAAACTTCGAAAAGGTAGGATAGAATTAAAAACTATGATTGGTAACTTGATATCGAATGTATTTCTTTCGAGTTCATTGCTAACTATGTATGTTGTTGAGAACAGGTGAGGGAATTAAAGCTACCAGTCATCTTATTAGAGGAAGAACTAATGGAAGGTGCCATGAATTGGCACAAACTTCTTGAAGCAGCAGATCGTGCAGGGAACAATTTTGTTAAAGAGGAGATTAAGCAAACTGATTTATGTGCACTTCCTTTCTCATCAGGAACCACAGGAGTTTCCAAAGGTGTTATGTTAACTCACCGAAATCTCGTAGCTAATATGTGTTCGACTCTCTCTGGTGTCCCGCAAGAAATGGAGGGCAAGGTTACGACGCTAGGCCTCATTCCATTCTTCCATATTTATGGGATCACTGGAATATGTTGCTCTACACTTAGAAACAAAGGAAAAGTTGTGGTGATGGGAAGATTTGATCTCAGGACTTTCATTAATGCTCTCATAACACATGAGATCACATTTGCTCCAATTGTTCCTCCTATTATACTGGCTTTGGTTAAGAATCCCATTGTGGAAGAATTTGATCTTAGCAGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCGCTCGCACCCGAACTTCAAACTGCCTTCGAGAGAAAGTTCCCCGGTGTGGACGTTCAAGAGGTAATTTAGTTGTTACTCGAGTGTAGTAGATTGACTGTGACAACTCTCCATTCTTTGTTCATTCCTTCAAAAATTTTAGTTATGCCATATAGTCCAATTTCCTGTTTTTGACATGTAAGAAGAGAATCAAGCTCTACCATCACATTGCAGAGTTAATCTTTGGGATTCTAACTTGTATAATATGCTAACTAATTGTCGTTGATCAACGACAGGCATACGGACTTACTGAGCACTGTTGCATCACTCTTAACTATGGAAGTATAGGGAAAGAGAATCTCAGTGCAAAGAAAAACACAGTTGGCCGTATTCTTCCTAATCTAGAAGTCAAATTCATCGACCCAGACACTGGAAGGTCTCTGCCGAAGAACACTCCAGGTGAAATCTGTGTAAGAAGCCAATGTGTGATGCAAGGTACAGAACATAGTTCGATGCAGAAAATTTTTACGGGATCTCTAACTAAATGCCAGCTGAATCTCTTCTTCTTGTTTTAGGCTACTACAAAAACGAAGAGGAGACTTCAAGGACGATCGATACCAAAGGCTGGATGCACACTGGGGATATTGGATACATCGATGACGATGGCAACGTGTTTATTGTGGATCGTATCAAGGAATTGATCAAGTACAAAGGATTTCAAGTAAAGACATAACATAACTTATTTCAAATTGATCCATTGATTGATAAAAAGGAAATTAAAGATGTGAAAAATGGAAGTTTGCATCAAACATATATGTGAACATGAGATAAGGTGATGGATGCATTGATTTCTCTGCAGGTTGCTCCAGCTGAGTTAGAGGCAATCCTTCTAACTCATCCCTCCATTGAAGATGCAGCTGTGGTGCCGTAAGAATTCTCCCCTCTCATTCATTTCGCTTCCTGTTGTTTCAATCTAAAGATAACAATGCTGTGCTGCATTGGCGTTTCAGGTTGCCAGATGAAGAAGCCGGTGAGATCCCAGCAGCAAGCGTTGTGATGGCTCCGAACTCAAAAGAAACCGAAGAAGATATAATCAAACACGTTGCCTCAAATGTTGCACATTACAAGAAAGTTAGACTCGTTCATTTTGTAGACACTATCCCAAAGTCGCCTTCTGGGAAAGTTATGAGAAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCTGATGAATCCGCTAGATTTATCTTCTAACTTTTATAATTGCCTGAGAGATGTTTGAGTTAAACCGTCATGTCTATATTTTACAAGATTTACAAGATGCTGTCCTAATATCAAGTTTGATCATTAATAACAGGGAACTAATACCAACAGGCTTTGATAAACAAAAGGTTACTTGATCCGTTGCTTTTAGAGTTACTTAAATTTGTTGAATTTTCTCAGTTTAATGCCTAAGCAAGGTGTTGAGTCTAAGGATTTAATCT

mRNA sequence

ATGCCAATCTCACCCTCAAGAACTCTACAAGTTTCTCATTCACAAAGCATGATACAACCTTTCTCAACTCTTCTTTTAAGTAGAGGGAAGTTGACGCCTGCAGAAAACTACTATTCTGCCATGGCTACTTGCAACCAAGATTCGGTACAAGACGAGGAACACATTTTCTGCAGCCAACTTCCTGAGGTCCAAGTGCCTTATGATATCACACTGCCTGAGTTTGTACTCCAGAATGCTGAATCATATGCCGAGAATGTGGCATTTGTGGAAGCCATGAGCGGAAAGGCATACACTTACCGTGAAGTTTTAAGAGACACAAATAGATTTTCCAAGGCCTTGCGCTCTCTGAGGTTGAAGAAAGGACATGTGGTTATTGTTGTTCTACCTAATGTTGCTGAATACGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCAGGTGTGAATCCGGCAGCTCACATATCAGAAATCAAAAAGCAAGTGGAGGTAGCAGAAGCAAAACTTGTCGTCACAAATGCCGCAAACTTCGAAAAGGTGAGGGAATTAAAGCTACCAGTCATCTTATTAGAGGAAGAACTAATGGAAGGTGCCATGAATTGGCACAAACTTCTTGAAGCAGCAGATCGTGCAGGGAACAATTTTGTTAAAGAGGAGATTAAGCAAACTGATTTATGTGCACTTCCTTTCTCATCAGGAACCACAGGAGTTTCCAAAGGTGTTATGTTAACTCACCGAAATCTCGTAGCTAATATGTGTTCGACTCTCTCTGGTGTCCCGCAAGAAATGGAGGGCAAGGTTACGACGCTAGGCCTCATTCCATTCTTCCATATTTATGGGATCACTGGAATATGTTGCTCTACACTTAGAAACAAAGGAAAAGTTGTGGTGATGGGAAGATTTGATCTCAGGACTTTCATTAATGCTCTCATAACACATGAGATCACATTTGCTCCAATTGTTCCTCCTATTATACTGGCTTTGGTTAAGAATCCCATTGTGGAAGAATTTGATCTTAGCAGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCGCTCGCACCCGAACTTCAAACTGCCTTCGAGAGAAAGTTCCCCGGTGTGGACGTTCAAGAGGCATACGGACTTACTGAGCACTGTTGCATCACTCTTAACTATGGAAGTATAGGGAAAGAGAATCTCAGTGCAAAGAAAAACACAGTTGGCCGTATTCTTCCTAATCTAGAAGTCAAATTCATCGACCCAGACACTGGAAGGTCTCTGCCGAAGAACACTCCAGGTGAAATCTGTGTAAGAAGCCAATGTGTGATGCAAGGCTACTACAAAAACGAAGAGGAGACTTCAAGGACGATCGATACCAAAGGCTGGATGCACACTGGGGATATTGGATACATCGATGACGATGGCAACGTGTTTATTGTGGATCGTATCAAGGAATTGATCAAGTACAAAGGATTTCAAGTTGCTCCAGCTGAGTTAGAGGCAATCCTTCTAACTCATCCCTCCATTGAAGATGCAGCTGTGGTGCCGTTGCCAGATGAAGAAGCCGGTGAGATCCCAGCAGCAAGCGTTGTGATGGCTCCGAACTCAAAAGAAACCGAAGAAGATATAATCAAACACGTTGCCTCAAATGTTGCACATTACAAGAAAGTTAGACTCGTTCATTTTGTAGACACTATCCCAAAGTCGCCTTCTGGGAAAGTTATGAGAAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCTGATGAATCCGCTAGATTTATCTTCTAA

Coding sequence (CDS)

ATGCCAATCTCACCCTCAAGAACTCTACAAGTTTCTCATTCACAAAGCATGATACAACCTTTCTCAACTCTTCTTTTAAGTAGAGGGAAGTTGACGCCTGCAGAAAACTACTATTCTGCCATGGCTACTTGCAACCAAGATTCGGTACAAGACGAGGAACACATTTTCTGCAGCCAACTTCCTGAGGTCCAAGTGCCTTATGATATCACACTGCCTGAGTTTGTACTCCAGAATGCTGAATCATATGCCGAGAATGTGGCATTTGTGGAAGCCATGAGCGGAAAGGCATACACTTACCGTGAAGTTTTAAGAGACACAAATAGATTTTCCAAGGCCTTGCGCTCTCTGAGGTTGAAGAAAGGACATGTGGTTATTGTTGTTCTACCTAATGTTGCTGAATACGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCAGGTGTGAATCCGGCAGCTCACATATCAGAAATCAAAAAGCAAGTGGAGGTAGCAGAAGCAAAACTTGTCGTCACAAATGCCGCAAACTTCGAAAAGGTGAGGGAATTAAAGCTACCAGTCATCTTATTAGAGGAAGAACTAATGGAAGGTGCCATGAATTGGCACAAACTTCTTGAAGCAGCAGATCGTGCAGGGAACAATTTTGTTAAAGAGGAGATTAAGCAAACTGATTTATGTGCACTTCCTTTCTCATCAGGAACCACAGGAGTTTCCAAAGGTGTTATGTTAACTCACCGAAATCTCGTAGCTAATATGTGTTCGACTCTCTCTGGTGTCCCGCAAGAAATGGAGGGCAAGGTTACGACGCTAGGCCTCATTCCATTCTTCCATATTTATGGGATCACTGGAATATGTTGCTCTACACTTAGAAACAAAGGAAAAGTTGTGGTGATGGGAAGATTTGATCTCAGGACTTTCATTAATGCTCTCATAACACATGAGATCACATTTGCTCCAATTGTTCCTCCTATTATACTGGCTTTGGTTAAGAATCCCATTGTGGAAGAATTTGATCTTAGCAGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCGCTCGCACCCGAACTTCAAACTGCCTTCGAGAGAAAGTTCCCCGGTGTGGACGTTCAAGAGGCATACGGACTTACTGAGCACTGTTGCATCACTCTTAACTATGGAAGTATAGGGAAAGAGAATCTCAGTGCAAAGAAAAACACAGTTGGCCGTATTCTTCCTAATCTAGAAGTCAAATTCATCGACCCAGACACTGGAAGGTCTCTGCCGAAGAACACTCCAGGTGAAATCTGTGTAAGAAGCCAATGTGTGATGCAAGGCTACTACAAAAACGAAGAGGAGACTTCAAGGACGATCGATACCAAAGGCTGGATGCACACTGGGGATATTGGATACATCGATGACGATGGCAACGTGTTTATTGTGGATCGTATCAAGGAATTGATCAAGTACAAAGGATTTCAAGTTGCTCCAGCTGAGTTAGAGGCAATCCTTCTAACTCATCCCTCCATTGAAGATGCAGCTGTGGTGCCGTTGCCAGATGAAGAAGCCGGTGAGATCCCAGCAGCAAGCGTTGTGATGGCTCCGAACTCAAAAGAAACCGAAGAAGATATAATCAAACACGTTGCCTCAAATGTTGCACATTACAAGAAAGTTAGACTCGTTCATTTTGTAGACACTATCCCAAAGTCGCCTTCTGGGAAAGTTATGAGAAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCTGATGAATCCGCTAGATTTATCTTCTAA

Protein sequence

MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLTPAENYYSAMATCNQDSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARFIF*
Homology
BLAST of CsaV3_5G030200 vs. NCBI nr
Match: XP_004135516.2 (4-coumarate--CoA ligase-like 1 [Cucumis sativus] >KAE8648676.1 hypothetical protein Csa_008120 [Cucumis sativus])

HSP 1 Score: 1163.7 bits (3009), Expect = 0.0e+00
Identity = 594/594 (100.00%), Postives = 594/594 (100.00%), Query Frame = 0

Query: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLTPAENYYSAMATCNQDSVQDEEHIFCSQL 60
           MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLTPAENYYSAMATCNQDSVQDEEHIFCSQL
Sbjct: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLTPAENYYSAMATCNQDSVQDEEHIFCSQL 60

Query: 61  PEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALRSLRLKK 120
           PEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALRSLRLKK
Sbjct: 61  PEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALRSLRLKK 120

Query: 121 GHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFE 180
           GHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFE
Sbjct: 121 GHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFE 180

Query: 181 KVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVS 240
           KVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVS
Sbjct: 181 KVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVS 240

Query: 241 KGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVM 300
           KGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVM
Sbjct: 241 KGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVM 300

Query: 301 GRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPEL 360
           GRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPEL
Sbjct: 301 GRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPEL 360

Query: 361 QTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKKNTVGRILPNLEVKFIDPDT 420
           QTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKKNTVGRILPNLEVKFIDPDT
Sbjct: 361 QTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKKNTVGRILPNLEVKFIDPDT 420

Query: 421 GRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVDRI 480
           GRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVDRI
Sbjct: 421 GRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVDRI 480

Query: 481 KELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEEDI 540
           KELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEEDI
Sbjct: 481 KELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEEDI 540

Query: 541 IKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARFIF 595
           IKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARFIF
Sbjct: 541 IKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARFIF 594

BLAST of CsaV3_5G030200 vs. NCBI nr
Match: XP_008446022.1 (PREDICTED: 4-coumarate--CoA ligase-like 1 [Cucumis melo])

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 577/596 (96.81%), Postives = 584/596 (97.99%), Query Frame = 0

Query: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKL--TPAENYYSAMATCNQDSVQDEEHIFCS 60
           MPISPSRTLQVSHSQSMIQPFSTLLLSRGKL    AENYYSAM T NQDSVQDE+HIF S
Sbjct: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRS 60

Query: 61  QLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALRSLRL 120
           QLPEVQVP DITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKAL SLRL
Sbjct: 61  QLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRL 120

Query: 121 KKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180
           KKGHVV+VVLPNVAEYAIVALGIM AGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN
Sbjct: 121 KKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180

Query: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240
           FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG
Sbjct: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240

Query: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300
           VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV
Sbjct: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300

Query: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360
           VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP
Sbjct: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360

Query: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKKNTVGRILPNLEVKFIDP 420
           ELQTAFERKFPGVDVQEAYGLTEHCCITLNYG+IGKENLSAKKNTVGRILPNLEVKFIDP
Sbjct: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420

Query: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVD 480
           DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTID+KGWMHTGDIGYIDDDG+VFIVD
Sbjct: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVD 480

Query: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEE 540
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNS ETEE
Sbjct: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEE 540

Query: 541 DIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARFIF 595
           +IIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESAR +F
Sbjct: 541 EIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF 596

BLAST of CsaV3_5G030200 vs. NCBI nr
Match: KAA0034187.1 (4-coumarate--CoA ligase-like 1 [Cucumis melo var. makuwa] >TYK15733.1 4-coumarate--CoA ligase-like 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 561/580 (96.72%), Postives = 568/580 (97.93%), Query Frame = 0

Query: 17  MIQPFSTLLLSRGKL--TPAENYYSAMATCNQDSVQDEEHIFCSQLPEVQVPYDITLPEF 76
           MIQPFSTLLLSRGKL    AENYYSAM T NQDSVQDE+HIF SQLPEVQVP DITLPEF
Sbjct: 1   MIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRSQLPEVQVPCDITLPEF 60

Query: 77  VLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEY 136
           VLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKAL SLRLKKGHVV+VVLPNVAEY
Sbjct: 61  VLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRLKKGHVVLVVLPNVAEY 120

Query: 137 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEE 196
           AIVALGIM AGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEE
Sbjct: 121 AIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEE 180

Query: 197 ELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM 256
           ELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM
Sbjct: 181 ELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM 240

Query: 257 CSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALIT 316
           CSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALIT
Sbjct: 241 CSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALIT 300

Query: 317 HEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQ 376
           HEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQ
Sbjct: 301 HEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQ 360

Query: 377 EAYGLTEHCCITLNYGSIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICV 436
           EAYGLTEHCCITLNYG+IGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICV
Sbjct: 361 EAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICV 420

Query: 437 RSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPA 496
           RSQCVMQGYYKNEEETSRTID+KGWMHTGDIGYIDDDG+VFIVDRIKELIKYKGFQVAPA
Sbjct: 421 RSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 480

Query: 497 ELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEEDIIKHVASNVAHYKKV 556
           ELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNS ETEE+IIKHVASNVAHYKKV
Sbjct: 481 ELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEEEIIKHVASNVAHYKKV 540

Query: 557 RLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARFIF 595
           RLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESAR +F
Sbjct: 541 RLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF 580

BLAST of CsaV3_5G030200 vs. NCBI nr
Match: XP_023541569.1 (4-coumarate--CoA ligase-like 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1025.4 bits (2650), Expect = 2.0e-295
Identity = 523/592 (88.34%), Postives = 562/592 (94.93%), Query Frame = 0

Query: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGK--LTPAENYYSAMATCNQDSVQDEEHIFCS 60
           MPISPS  LQ+SH QSMIQ FS   L RG+  L  A    SAMATC +DSV+DEEHIF S
Sbjct: 15  MPISPSVALQISHPQSMIQAFSAPPLRRGRRSLMSAATNNSAMATCIRDSVEDEEHIFRS 74

Query: 61  QLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALRSLRL 120
           QLPEVQVP DITLPEFVLQNAESYA+NVAFVEA+SG AYTYREV+RDT+RF+KAL SLRL
Sbjct: 75  QLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGNAYTYREVVRDTSRFAKALSSLRL 134

Query: 121 KKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180
           KKG VVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVA+AKLVVTN+++
Sbjct: 135 KKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSS 194

Query: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240
           FEKV+ELKLPVI++ EEL+EG+MNWHKLLEAADRAGNNFVKE+IKQTDLCALPFSSGTTG
Sbjct: 195 FEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTG 254

Query: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300
           VSKGVMLTHRNLVAN+CSTLSGVPQEM GKVTTLGLIPFFHIYGITGICC+TLRNKGKVV
Sbjct: 255 VSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVV 314

Query: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360
           VMGRFDLRTFINALIT E+TFAPIVPPIILALVKNPIV+EFDLS LKLQAIMTAAAPLAP
Sbjct: 315 VMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAP 374

Query: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKKNTVGRILPNLEVKFIDP 420
           ELQT+FE+KFPGVDVQEAYGLTEH CITLNYGSIGK+NL+AKKNTVGRILPNLEVKFIDP
Sbjct: 375 ELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILPNLEVKFIDP 434

Query: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVD 480
           D+GRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID KGWMHTGDIGYIDDDG+VFIVD
Sbjct: 435 DSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVD 494

Query: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEE 540
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAGEIPAAS+VMAPN+KETE+
Sbjct: 495 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAKETED 554

Query: 541 DIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESA 591
           +IIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKMIEKIRA+ ++
Sbjct: 555 EIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 606

BLAST of CsaV3_5G030200 vs. NCBI nr
Match: XP_022945246.1 (4-coumarate--CoA ligase-like 1 [Cucurbita moschata])

HSP 1 Score: 1025.0 bits (2649), Expect = 2.6e-295
Identity = 523/592 (88.34%), Postives = 562/592 (94.93%), Query Frame = 0

Query: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGK--LTPAENYYSAMATCNQDSVQDEEHIFCS 60
           MPISPS  LQ+SH QSMIQ FS   L RG+     A N  SAMATC +D V+DEEHIF S
Sbjct: 15  MPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDLVEDEEHIFRS 74

Query: 61  QLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALRSLRL 120
           QLPEVQVP DITLPEFVLQNAESYA+NVAFVEA+SGKAYTYREV+RDT+RF+KAL SLRL
Sbjct: 75  QLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRL 134

Query: 121 KKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180
           KKG VVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVA+AKLVVTN+++
Sbjct: 135 KKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSS 194

Query: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240
           FEKV+ELKLPVI++ EEL+EG+MNWHKLLEAADRAGNNFVKE+IKQTDLCALPFSSGTTG
Sbjct: 195 FEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTG 254

Query: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300
           VSKGVMLTHRNLVAN+CSTLSGVPQEM GKVTTLGLIPFFHIYGITGICC+TLRNKGKVV
Sbjct: 255 VSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVV 314

Query: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360
           VMGRFDLRTFINALIT E+TFAPIVPPIILALVKNPIV+EFDLS LKLQAIMTAAAPLAP
Sbjct: 315 VMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAP 374

Query: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKKNTVGRILPNLEVKFIDP 420
           ELQT+FE+KFPGVDVQEAYGLTEH CITLNYGSIGK+NL+AKKNTVGRILPNLEVKFIDP
Sbjct: 375 ELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILPNLEVKFIDP 434

Query: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVD 480
           D+GRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID KGWMHTGDIGYIDDDG+VFIVD
Sbjct: 435 DSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVD 494

Query: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEE 540
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAGEIPAAS+VMAPN+KETE+
Sbjct: 495 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAKETED 554

Query: 541 DIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESA 591
           +IIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKMIEKIRA+ ++
Sbjct: 555 EIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAEAAS 606

BLAST of CsaV3_5G030200 vs. ExPASy Swiss-Prot
Match: Q9LQ12 (4-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 2.7e-223
Identity = 386/532 (72.56%), Postives = 451/532 (84.77%), Query Frame = 0

Query: 50  QDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRF 109
           +D E+IF S  P V +P  +TLPEFVLQ  E Y ENVAFVEA++GKA TY +V+RDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 110 SKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEA 169
           +KAL SL L+KG V++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 170 KLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCA 229
           + ++T+A N+EKV+ L LPVI+L EE +EGA+NW  LLEA D+ G+    EEI QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 230 LPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCS 289
           LPFSSGTTG+ KGVMLTHRNL+AN+CSTL GV  EM G++ TLGLIPFFHIYGI GICC+
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 290 TLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAI 349
           T++NKGKVV M R+DLR F+NALI HE++FAPIVPPIIL LVKNPIV+EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 350 MTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKKNTVGRILP 409
           MTAAAPLAPEL TAFE KFP V VQEAYGLTEH CITL +G   K    AK+N+VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 410 NLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYID 469
           NLEVKFIDPDTGRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID +GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 470 DDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVM 529
           DDG++FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPDEEAGEIPAA VV+
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 530 APNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMI 582
            P + E EEDI+  VA+NVAHYKKVR VHFVD+IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of CsaV3_5G030200 vs. ExPASy Swiss-Prot
Match: Q7XXL2 (4-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9 PE=2 SV=2)

HSP 1 Score: 696.0 bits (1795), Expect = 3.6e-199
Identity = 344/539 (63.82%), Postives = 439/539 (81.45%), Query Frame = 0

Query: 50  QDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMS-GKAYTYREVLRDTNR 109
           +++EH+F S+ P V VP  +T+PEFVL  AE+YA+ VA VEA + G++YTY EV RDT R
Sbjct: 14  EEQEHVFRSRFPPVAVPDGVTVPEFVLDGAEAYADRVALVEAAAGGRSYTYGEVARDTAR 73

Query: 110 FSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAE 169
           F++ALRS+ ++KGHVV+V LPN+A Y +V+LGIM+AG VFSGVNP A  +EIKKQVE +E
Sbjct: 74  FARALRSVGVRKGHVVVVALPNLAVYPVVSLGIMSAGAVFSGVNPRALAAEIKKQVEDSE 133

Query: 170 AKLVVTNAANFEKVRELKLPVI-LLEEELMEGAMNWHKLLEAADRAGNNFVK-EEIKQTD 229
           AKLVV N   F+KV++  +PVI + + E M GA++W  LL AADR G   V  +  +Q+D
Sbjct: 134 AKLVVANEVAFDKVKDAGVPVIGVGDRERMPGAISWDGLLAAADRTGAGVVPVDAAQQSD 193

Query: 230 LCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGI 289
           LCALP+SSGTTGVSKGVML+HRNLV+N+CS++  V  E  G+V TLGL+PFFHIYGITGI
Sbjct: 194 LCALPYSSGTTGVSKGVMLSHRNLVSNLCSSMFAVAPETAGQVVTLGLMPFFHIYGITGI 253

Query: 290 CCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKL 349
           CC+TLR+KG VVVM RFDLRTF+ AL+ H + FAP+VPP++LA+VK+P+ +EFDLS L L
Sbjct: 254 CCATLRHKGTVVVMDRFDLRTFLRALVDHRVMFAPLVPPVMLAMVKSPVADEFDLSDLAL 313

Query: 350 QAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKKNTVGR 409
           +++MTAAAPLAP+L  AF+RKFPGV V+EAYGLTEH CITL + +       AKK++VG 
Sbjct: 314 KSVMTAAAPLAPDLLAAFQRKFPGVQVEEAYGLTEHSCITLTHAAGDGHGHVAKKSSVGF 373

Query: 410 ILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIG 469
           ILPNLEVKF+DPDTGRSLP NTPGE+CVRSQ VMQGYYK +EET RT+D KGW+HTGD+G
Sbjct: 374 ILPNLEVKFVDPDTGRSLPANTPGELCVRSQSVMQGYYKRKEETERTVDGKGWLHTGDVG 433

Query: 470 YIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAAS 529
           YID DG+VFIVDRIKELIKYKGFQVAPAELEA+LL+HPS+EDAAV  +PDEEAGE+P A 
Sbjct: 434 YIDGDGDVFIVDRIKELIKYKGFQVAPAELEAVLLSHPSVEDAAVFGVPDEEAGEVPVAC 493

Query: 530 VVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIR 586
           VV    ++E EE+I+ +VA  VA YK+VR++H VD IPKS SGK++RR ++++ I++++
Sbjct: 494 VVRRHGAEEGEEEIVAYVAERVASYKRVRVLHIVDAIPKSVSGKILRRQLRDEFIKRMK 552

BLAST of CsaV3_5G030200 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 8.8e-113
Identity = 203/539 (37.66%), Postives = 332/539 (61.60%), Query Frame = 0

Query: 47  DSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDT 106
           ++ Q  + IF S+LP++ +P  + L  +  +N   +      ++  + + YTY EV   +
Sbjct: 6   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTS 65

Query: 107 NRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEV 166
            + +  L  L +++   ++++LPN  E+    +G    G + +  NP    +E+ KQ + 
Sbjct: 66  RKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKA 125

Query: 167 AEAKLVVTNAANFEKVRELKLP---VILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIK 226
           + AK+V+T A    KV++  +     ++  + + EG +++ +L+++ +   +     +I+
Sbjct: 126 SSAKIVITQACFAGKVKDYAIENDLKVICVDSVPEGCVHFSELIQSDE---HEIPDVKIQ 185

Query: 227 QTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQE--MEGKVTTLGLIPFFHIY 286
             D+ ALP+SSGTTG+ KGVMLTH+ LV ++   + G      M      + ++P FHIY
Sbjct: 186 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 245

Query: 287 GITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDL 346
            +  +    LR    +++M +FD+  F+  +  H++T  P VPPI+LA+ K+P+V+ +DL
Sbjct: 246 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDL 305

Query: 347 SSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKK 406
           SS++   +M+ AAPL  EL+ A   KFP   + + YG+TE   +     +  KE    K 
Sbjct: 306 SSVR--TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKS 365

Query: 407 NTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMH 466
              G ++ N E+K +DPDTG SLP+N PGEIC+R   +M+GY  + E T+RTI+ +GW+H
Sbjct: 366 GACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLH 425

Query: 467 TGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGE 526
           TGDIG+IDDD  +FIVDR+KELIKYKGFQVAPAELEA+L+ HP I DAAVVP+ DE+AGE
Sbjct: 426 TGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGE 485

Query: 527 IPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 581
           +P A VV +  S  TE+++   ++  V  YK+++ V FV+T+PKSPSGK++R+ ++ ++
Sbjct: 486 VPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARL 539

BLAST of CsaV3_5G030200 vs. ExPASy Swiss-Prot
Match: P14912 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 1.5e-112
Identity = 209/534 (39.14%), Postives = 330/534 (61.80%), Query Frame = 0

Query: 52  EEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSK 111
           E+ IF S+LP++ +P  + L  +  +N     +    +   +G+ +TY +V   + + + 
Sbjct: 9   EDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 68

Query: 112 ALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKL 171
            L  L +++G  ++++LPN  EY    LG    G + +  NP    +E+ KQ++ ++AKL
Sbjct: 69  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKL 128

Query: 172 VVTNAANFEKVREL---KLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLC 231
           ++T A   +KV++    K   I+  ++  +  +++ KL+EA +   +   +  I   D+ 
Sbjct: 129 IITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADE---SEMPEVVINSDDVV 188

Query: 232 ALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQE--MEGKVTTLGLIPFFHIYGITGI 291
           ALP+SSGTTG+ KGVMLTH+ LV ++   + G      M  +   + ++P FHIY +  +
Sbjct: 189 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 248

Query: 292 CCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKL 351
            C  LR    +++M +FD+  F+  +  +++T  P VPPI+LA+ K+P+V+++DLSS++ 
Sbjct: 249 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVR- 308

Query: 352 QAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKKNTVGR 411
             +M+ AAPL  EL+ A   KFP   + + YG+TE   +     +  KE    K    G 
Sbjct: 309 -TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGT 368

Query: 412 ILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIG 471
           ++ N E+K +DP+T  SLP+N  GEIC+R   +M+GY  + E T  TID +GW+HTGDIG
Sbjct: 369 VVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIG 428

Query: 472 YIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAAS 531
           +IDDD  +FIVDR+KE+IKYKGFQVAPAELEA+LLTHP+I DAAVVP+ DE+AGE+P A 
Sbjct: 429 FIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAF 488

Query: 532 VVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 581
           VV       TEE+I + V+  V  YK++  V FVD IPKSPSGK++R+ ++ ++
Sbjct: 489 VVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARI 537

BLAST of CsaV3_5G030200 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 2.5e-112
Identity = 203/539 (37.66%), Postives = 330/539 (61.22%), Query Frame = 0

Query: 47  DSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDT 106
           ++ Q  + IF S+LP++ +P  + L  +  +N   +      ++  + + YTY EV   +
Sbjct: 6   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTS 65

Query: 107 NRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEV 166
            + +  L  L +++   ++++LPN  E+    +G    G + +  NP    +E+ KQ + 
Sbjct: 66  RKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKA 125

Query: 167 AEAKLVVTNAANFEKVRELKLP---VILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIK 226
           + AK+V+T A    KV++  +     ++  +   EG +++ +L+++ +   +     +I+
Sbjct: 126 SSAKIVITQACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDE---HEIPDVKIQ 185

Query: 227 QTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQE--MEGKVTTLGLIPFFHIY 286
             D+ ALP+SSGTTG+ KGVMLTH+ LV ++   + G      M      + ++P FHIY
Sbjct: 186 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 245

Query: 287 GITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDL 346
            +  +    LR    +++M +FD+  F+  +  H++T  P VPPI+LA+ K+P+V  +DL
Sbjct: 246 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDL 305

Query: 347 SSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKK 406
           SS++   +M+ AAPL  EL+ A   KFP   + + YG+TE   +     +  KE    K 
Sbjct: 306 SSVR--TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKS 365

Query: 407 NTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMH 466
              G ++ N E+K +DPDTG SLP+N PGEIC+R   +M+GY  + E T+RTI+ +GW+H
Sbjct: 366 GACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLH 425

Query: 467 TGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGE 526
           TGDIG+IDDD  +FIVDR+KELIKYKGFQVAPAELEA+L+ HP I DAAVVP+ DE+AGE
Sbjct: 426 TGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGE 485

Query: 527 IPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 581
           +P A VV +  S  TE+++   ++  V  YK+++ V FV+T+PKSPSGK++R+ ++ ++
Sbjct: 486 VPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARL 539

BLAST of CsaV3_5G030200 vs. ExPASy TrEMBL
Match: A0A1S3BER3 (4-coumarate--CoA ligase-like 1 OS=Cucumis melo OX=3656 GN=LOC103488874 PE=4 SV=1)

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 577/596 (96.81%), Postives = 584/596 (97.99%), Query Frame = 0

Query: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKL--TPAENYYSAMATCNQDSVQDEEHIFCS 60
           MPISPSRTLQVSHSQSMIQPFSTLLLSRGKL    AENYYSAM T NQDSVQDE+HIF S
Sbjct: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRS 60

Query: 61  QLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALRSLRL 120
           QLPEVQVP DITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKAL SLRL
Sbjct: 61  QLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRL 120

Query: 121 KKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180
           KKGHVV+VVLPNVAEYAIVALGIM AGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN
Sbjct: 121 KKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180

Query: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240
           FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG
Sbjct: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240

Query: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300
           VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV
Sbjct: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300

Query: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360
           VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP
Sbjct: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360

Query: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKKNTVGRILPNLEVKFIDP 420
           ELQTAFERKFPGVDVQEAYGLTEHCCITLNYG+IGKENLSAKKNTVGRILPNLEVKFIDP
Sbjct: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420

Query: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVD 480
           DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTID+KGWMHTGDIGYIDDDG+VFIVD
Sbjct: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVD 480

Query: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEE 540
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNS ETEE
Sbjct: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEE 540

Query: 541 DIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARFIF 595
           +IIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESAR +F
Sbjct: 541 EIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF 596

BLAST of CsaV3_5G030200 vs. ExPASy TrEMBL
Match: A0A5D3CV41 (4-coumarate--CoA ligase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002330 PE=4 SV=1)

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 561/580 (96.72%), Postives = 568/580 (97.93%), Query Frame = 0

Query: 17  MIQPFSTLLLSRGKL--TPAENYYSAMATCNQDSVQDEEHIFCSQLPEVQVPYDITLPEF 76
           MIQPFSTLLLSRGKL    AENYYSAM T NQDSVQDE+HIF SQLPEVQVP DITLPEF
Sbjct: 1   MIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRSQLPEVQVPCDITLPEF 60

Query: 77  VLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEY 136
           VLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKAL SLRLKKGHVV+VVLPNVAEY
Sbjct: 61  VLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRLKKGHVVLVVLPNVAEY 120

Query: 137 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEE 196
           AIVALGIM AGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEE
Sbjct: 121 AIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEE 180

Query: 197 ELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM 256
           ELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM
Sbjct: 181 ELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM 240

Query: 257 CSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALIT 316
           CSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALIT
Sbjct: 241 CSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALIT 300

Query: 317 HEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQ 376
           HEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQ
Sbjct: 301 HEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQ 360

Query: 377 EAYGLTEHCCITLNYGSIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICV 436
           EAYGLTEHCCITLNYG+IGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICV
Sbjct: 361 EAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICV 420

Query: 437 RSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPA 496
           RSQCVMQGYYKNEEETSRTID+KGWMHTGDIGYIDDDG+VFIVDRIKELIKYKGFQVAPA
Sbjct: 421 RSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 480

Query: 497 ELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEEDIIKHVASNVAHYKKV 556
           ELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNS ETEE+IIKHVASNVAHYKKV
Sbjct: 481 ELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEEEIIKHVASNVAHYKKV 540

Query: 557 RLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARFIF 595
           RLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESAR +F
Sbjct: 541 RLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF 580

BLAST of CsaV3_5G030200 vs. ExPASy TrEMBL
Match: A0A0A0KRZ5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G589320 PE=4 SV=1)

HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 554/554 (100.00%), Postives = 554/554 (100.00%), Query Frame = 0

Query: 41  MATCNQDSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYR 100
           MATCNQDSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYR
Sbjct: 1   MATCNQDSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYR 60

Query: 101 EVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 160
           EVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 61  EVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120

Query: 161 KKQVEVAEAKLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKE 220
           KKQVEVAEAKLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKE
Sbjct: 121 KKQVEVAEAKLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKE 180

Query: 221 EIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHI 280
           EIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHI
Sbjct: 181 EIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHI 240

Query: 281 YGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFD 340
           YGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFD
Sbjct: 241 YGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFD 300

Query: 341 LSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAK 400
           LSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAK
Sbjct: 301 LSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAK 360

Query: 401 KNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWM 460
           KNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWM
Sbjct: 361 KNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWM 420

Query: 461 HTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 520
           HTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG
Sbjct: 421 HTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480

Query: 521 EIPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 580
           EIPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM
Sbjct: 481 EIPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 540

Query: 581 IEKIRADESARFIF 595
           IEKIRADESARFIF
Sbjct: 541 IEKIRADESARFIF 554

BLAST of CsaV3_5G030200 vs. ExPASy TrEMBL
Match: A0A6J1G0B4 (4-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=4 SV=1)

HSP 1 Score: 1025.0 bits (2649), Expect = 1.2e-295
Identity = 523/592 (88.34%), Postives = 562/592 (94.93%), Query Frame = 0

Query: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGK--LTPAENYYSAMATCNQDSVQDEEHIFCS 60
           MPISPS  LQ+SH QSMIQ FS   L RG+     A N  SAMATC +D V+DEEHIF S
Sbjct: 15  MPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDLVEDEEHIFRS 74

Query: 61  QLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALRSLRL 120
           QLPEVQVP DITLPEFVLQNAESYA+NVAFVEA+SGKAYTYREV+RDT+RF+KAL SLRL
Sbjct: 75  QLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRL 134

Query: 121 KKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180
           KKG VVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVA+AKLVVTN+++
Sbjct: 135 KKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSS 194

Query: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240
           FEKV+ELKLPVI++ EEL+EG+MNWHKLLEAADRAGNNFVKE+IKQTDLCALPFSSGTTG
Sbjct: 195 FEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTG 254

Query: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300
           VSKGVMLTHRNLVAN+CSTLSGVPQEM GKVTTLGLIPFFHIYGITGICC+TLRNKGKVV
Sbjct: 255 VSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVV 314

Query: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360
           VMGRFDLRTFINALIT E+TFAPIVPPIILALVKNPIV+EFDLS LKLQAIMTAAAPLAP
Sbjct: 315 VMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAP 374

Query: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKKNTVGRILPNLEVKFIDP 420
           ELQT+FE+KFPGVDVQEAYGLTEH CITLNYGSIGK+NL+AKKNTVGRILPNLEVKFIDP
Sbjct: 375 ELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILPNLEVKFIDP 434

Query: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVD 480
           D+GRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID KGWMHTGDIGYIDDDG+VFIVD
Sbjct: 435 DSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVD 494

Query: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEE 540
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAGEIPAAS+VMAPN+KETE+
Sbjct: 495 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAKETED 554

Query: 541 DIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESA 591
           +IIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKMIEKIRA+ ++
Sbjct: 555 EIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAEAAS 606

BLAST of CsaV3_5G030200 vs. ExPASy TrEMBL
Match: A0A6J1HUV5 (4-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 SV=1)

HSP 1 Score: 1022.7 bits (2643), Expect = 6.2e-295
Identity = 519/592 (87.67%), Postives = 562/592 (94.93%), Query Frame = 0

Query: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLT--PAENYYSAMATCNQDSVQDEEHIFCS 60
           MPISPS  LQ+SH QSMIQ FS   L RG+ +   A N  S MATC +DS +DEEHIF S
Sbjct: 15  MPISPSVALQISHPQSMIQAFSAPPLRRGRRSSMSAANNNSTMATCIRDSAEDEEHIFRS 74

Query: 61  QLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALRSLRL 120
           QLPEVQVP DITLPEFVLQNAE YA+NVAFVEA+SGKAYTYREV+RDT+RF+KAL SLRL
Sbjct: 75  QLPEVQVPDDITLPEFVLQNAELYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRL 134

Query: 121 KKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180
           KKG +VIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVA+AKLVVTN+++
Sbjct: 135 KKGQIVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSS 194

Query: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240
           FEKV+ELKLPVI++ EEL+EG+MNWHKLLEAADRAGNNFVKE+IKQTDLCALPFSSGTTG
Sbjct: 195 FEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTG 254

Query: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300
           VSKGVMLTHRNLVAN+CSTLSG+PQEM GKVTTLGLIPFFHIYGITGICC+TLRNKGKVV
Sbjct: 255 VSKGVMLTHRNLVANLCSTLSGIPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVV 314

Query: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360
           VMGRFDLRTFINALIT E+TFAPIVPPIILALVKNPIV+EFDLSSLKLQAIMTAAAPLAP
Sbjct: 315 VMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSSLKLQAIMTAAAPLAP 374

Query: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKKNTVGRILPNLEVKFIDP 420
           ELQT+FE+KFPGVDVQEAYGLTEH CITLNYG+IGK+NL+AKKNTVGRILPNLEVKFIDP
Sbjct: 375 ELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGNIGKDNLAAKKNTVGRILPNLEVKFIDP 434

Query: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVD 480
           D+GRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID KGWMHTGDIGYIDDDG+VFIVD
Sbjct: 435 DSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVD 494

Query: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEE 540
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAGEIPAAS+VMAPN+KETE+
Sbjct: 495 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAKETED 554

Query: 541 DIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESA 591
           +IIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKMIEKIRA+ ++
Sbjct: 555 EIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 606

BLAST of CsaV3_5G030200 vs. TAIR 10
Match: AT1G62940.1 (acyl-CoA synthetase 5 )

HSP 1 Score: 776.2 bits (2003), Expect = 1.9e-224
Identity = 386/532 (72.56%), Postives = 451/532 (84.77%), Query Frame = 0

Query: 50  QDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRF 109
           +D E+IF S  P V +P  +TLPEFVLQ  E Y ENVAFVEA++GKA TY +V+RDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 110 SKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEA 169
           +KAL SL L+KG V++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 170 KLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCA 229
           + ++T+A N+EKV+ L LPVI+L EE +EGA+NW  LLEA D+ G+    EEI QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 230 LPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCS 289
           LPFSSGTTG+ KGVMLTHRNL+AN+CSTL GV  EM G++ TLGLIPFFHIYGI GICC+
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 290 TLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAI 349
           T++NKGKVV M R+DLR F+NALI HE++FAPIVPPIIL LVKNPIV+EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 350 MTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKKNTVGRILP 409
           MTAAAPLAPEL TAFE KFP V VQEAYGLTEH CITL +G   K    AK+N+VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 410 NLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYID 469
           NLEVKFIDPDTGRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID +GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 470 DDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVM 529
           DDG++FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPDEEAGEIPAA VV+
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 530 APNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMI 582
            P + E EEDI+  VA+NVAHYKKVR VHFVD+IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of CsaV3_5G030200 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 392.9 bits (1008), Expect = 4.6e-109
Identity = 200/532 (37.59%), Postives = 326/532 (61.28%), Query Frame = 0

Query: 55  IFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALR 114
           IF S+LP++ +P  + L +++ +N   +A     +   +G+ YTY +V   + + +  L 
Sbjct: 22  IFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLAAGLH 81

Query: 115 SLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVT 174
           +L +K+  VV+++LPN  E  +  L     G + +  NP    +EI KQ + + AKL+VT
Sbjct: 82  NLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKLIVT 141

Query: 175 NAANFEKVRELKLPVILL----EEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCAL 234
            +   +K++ L+   +L+     + + E  + + +L ++ +   ++ + E+I   D+ AL
Sbjct: 142 QSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDS-IPEKISPEDVVAL 201

Query: 235 PFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEM--EGKVTTLGLIPFFHIYGITGICC 294
           PFSSGTTG+ KGVMLTH+ LV ++   + G    +        L ++P FHIY +  I  
Sbjct: 202 PFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNSIML 261

Query: 295 STLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQA 354
            +LR    +++M +F++   +  +   ++T A +VPPI+LA+ K+P  E++DLSS+++  
Sbjct: 262 CSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVRM-- 321

Query: 355 IMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKKNTVGRIL 414
           + + AAPL  EL+ A   KFP   + + YG+TE   +        KE    K    G ++
Sbjct: 322 VKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVV 381

Query: 415 PNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYI 474
            N E+K +DPDTG SLP+N PGEIC+R   +M+GY  +   T+ TID  GW+HTGD+G+I
Sbjct: 382 RNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFI 441

Query: 475 DDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVV 534
           DDD  +FIVDR+KELIKYKGFQVAPAELE++L+ HP I D AVV + +E+AGE+P A VV
Sbjct: 442 DDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVV 501

Query: 535 MAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 581
            + +S  +E++I + V+  V  YK++  V F D+IPK+PSGK++R+ ++ ++
Sbjct: 502 RSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARL 550

BLAST of CsaV3_5G030200 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 387.5 bits (994), Expect = 1.9e-107
Identity = 201/545 (36.88%), Postives = 322/545 (59.08%), Query Frame = 0

Query: 46  QDSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRD 105
           Q +  + + IF S+LP++ +P  ++L +++ QN   +A     +   +G  YTY +V   
Sbjct: 15  QSNNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVI 74

Query: 106 TNRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVE 165
           + + +     L + +  VV+++LPN  E+ +  L     G   +  NP    +EI KQ +
Sbjct: 75  SRQIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAK 134

Query: 166 VAEAKLVVTNAANFEKVRELK----LPVILLEEE----LMEGAMNWHKLLEAADRAGNNF 225
            +  KL++T A   +K++ L+    + ++ +++     + EG + + +L ++   A    
Sbjct: 135 ASNTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVI 194

Query: 226 VKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEM--EGKVTTLGLI 285
              EI   D+ ALP+SSGTTG+ KGVMLTH+ LV ++   + G    +        L ++
Sbjct: 195 DSVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVL 254

Query: 286 PFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPI 345
           P FHIY +  I    LR    +++M +F++   +  +   ++T AP+VPPI+LA+ K+  
Sbjct: 255 PMFHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSE 314

Query: 346 VEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKE 405
            E++DLSS+++  + + AAPL  EL+ A   KFP   + + YG+TE   +        KE
Sbjct: 315 TEKYDLSSIRV--VKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKE 374

Query: 406 NLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTID 465
               K    G ++ N E+K +DPDTG SL +N PGEIC+R   +M+GY  N   T+ TID
Sbjct: 375 PFPVKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETID 434

Query: 466 TKGWMHTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLP 525
             GW+HTGDIG IDDD  +FIVDR+KELIKYKGFQVAPAELEA+L+ HP I D AVV + 
Sbjct: 435 KDGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMK 494

Query: 526 DEEAGEIPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRL 581
           +E AGE+P A VV + +S+ +E+D+ + V+  V  YK++  V F ++IPK+PSGK++R+ 
Sbjct: 495 EEAAGEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKD 554

BLAST of CsaV3_5G030200 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 380.9 bits (977), Expect = 1.8e-105
Identity = 204/531 (38.42%), Postives = 322/531 (60.64%), Query Frame = 0

Query: 55  IFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALR 114
           IF S+LP++ +P  + L  +  +   S ++    +   +GK+YTY E      R +  L 
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 115 SLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVT 174
            L ++KG V++++L N AE+    +G    G V +  NP     E+ KQ++ + AKL++T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 175 NAANFEKVREL--KLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKE-EIKQTDLCALP 234
           ++   +K++ L   L +I  +E   E  + +  L+   D   N F +  +I   D  ALP
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLI--TDDETNPFQETVDIGGDDAAALP 213

Query: 235 FSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQE--MEGKVTTLGLIPFFHIYGITGICCS 294
           FSSGTTG+ KGV+LTH++L+ ++   + G      ++     L ++P FHIY +  +  +
Sbjct: 214 FSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLN 273

Query: 295 TLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAI 354
           +LR+   V++M +F++   ++ +  H +T A +VPP+++AL KNP V  +DLSS++   +
Sbjct: 274 SLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRF--V 333

Query: 355 MTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKKNTVGRILP 414
           ++ AAPL  ELQ +  R+ P   + + YG+TE   +        KE +  K  + G ++ 
Sbjct: 334 LSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVR 393

Query: 415 NLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYID 474
           N E+K +  +T  SL  N PGEIC+R Q +M+ Y  + E TS TID +GW+HTGDIGY+D
Sbjct: 394 NAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVD 453

Query: 475 DDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVM 534
           +D  +FIVDR+KE+IK+KGFQV PAELE++L+ H SI DAAVVP  DE AGE+P A VV 
Sbjct: 454 EDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVR 513

Query: 535 APNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 581
           +  +  TEED+ ++VA  V  YK++  V FV +IPKSPSGK++R+ +K K+
Sbjct: 514 SNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of CsaV3_5G030200 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 377.1 bits (967), Expect = 2.6e-104
Identity = 197/554 (35.56%), Postives = 331/554 (59.75%), Query Frame = 0

Query: 46  QDSVQDEEH--IFCSQLPEVQVPYDITLPEFVLQ----NAESYAENVAFVEAMSGKAYTY 105
           +D  ++  H  IF S+LP++ +P  + L ++V Q    + +  +     ++  +G+  TY
Sbjct: 17  EDEEEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTY 76

Query: 106 REVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISE 165
            +V  +  R +  +  L ++ G VV+++LPN  E+A+  L +   G V +  NP     E
Sbjct: 77  ADVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPE 136

Query: 166 IKKQVEVAEAKLVVTNAANFEKVRELK---LPVILLEEE--------LMEGAMNWHKLLE 225
           I KQ + + AK+++T     +K+  LK   + ++ L+++          +G +++ +L +
Sbjct: 137 IAKQAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQ 196

Query: 226 AADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEME-- 285
           A +      +K +I   D  A+P+SSGTTG+ KGVM+TH+ LV ++   + G    +   
Sbjct: 197 ADE---TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFT 256

Query: 286 GKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPI 345
                L  +P FHIY +  +  S +R    ++++ RF+L   +  +  +++T  P+ PP+
Sbjct: 257 ANDVILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPV 316

Query: 346 ILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCIT 405
           +LA +K+P  E +DLSS+++  +++ AA L  EL+ A   KFP     + YG+TE   + 
Sbjct: 317 VLAFIKSPETERYDLSSVRI--MLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTVA 376

Query: 406 LNYGSIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKN 465
            +  +  K     K    G ++ N E+K +D +TG SLP+N  GEICVR   +M+GY  +
Sbjct: 377 KSL-AFAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLND 436

Query: 466 EEETSRTIDTKGWMHTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSI 525
            E T+RTID  GW+HTGDIG++DDD  +FIVDR+KELIK+KG+QVAPAELEA+L++HPSI
Sbjct: 437 PEATARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSI 496

Query: 526 EDAAVVPLPDEEAGEIPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKS 581
           +DAAVV + DE A E+P A V  +  S+ TE+D+  +V   V HYK++++V F++ IPK+
Sbjct: 497 DDAAVVAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKA 556

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004135516.20.0e+00100.004-coumarate--CoA ligase-like 1 [Cucumis sativus] >KAE8648676.1 hypothetical prot... [more]
XP_008446022.10.0e+0096.81PREDICTED: 4-coumarate--CoA ligase-like 1 [Cucumis melo][more]
KAA0034187.10.0e+0096.724-coumarate--CoA ligase-like 1 [Cucumis melo var. makuwa] >TYK15733.1 4-coumarat... [more]
XP_023541569.12.0e-29588.344-coumarate--CoA ligase-like 1 [Cucurbita pepo subsp. pepo][more]
XP_022945246.12.6e-29588.344-coumarate--CoA ligase-like 1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9LQ122.7e-22372.564-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=... [more]
Q7XXL23.6e-19963.824-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9... [more]
P316848.8e-11337.664-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1[more]
P149121.5e-11239.144-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1[more]
P316852.5e-11237.664-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BER30.0e+0096.814-coumarate--CoA ligase-like 1 OS=Cucumis melo OX=3656 GN=LOC103488874 PE=4 SV=1[more]
A0A5D3CV410.0e+0096.724-coumarate--CoA ligase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A0A0KRZ50.0e+00100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G589320 PE=4 SV=1[more]
A0A6J1G0B41.2e-29588.344-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=... [more]
A0A6J1HUV56.2e-29587.674-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT1G62940.11.9e-22472.56acyl-CoA synthetase 5 [more]
AT3G21240.14.6e-10937.594-coumarate:CoA ligase 2 [more]
AT1G51680.11.9e-10736.884-coumarate:CoA ligase 1 [more]
AT1G65060.11.8e-10538.424-coumarate:CoA ligase 3 [more]
AT3G21230.12.6e-10435.564-coumarate:CoA ligase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 495..570
e-value: 9.5E-18
score: 65.0
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 77..486
e-value: 1.0E-98
score: 330.7
NoneNo IPR availableGENE3D3.30.300.30coord: 482..584
e-value: 1.9E-31
score: 110.7
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 47..583
NoneNo IPR availablePANTHERPTHR24096:SF3894-COUMARATE--COA LIGASE-LIKE 1coord: 47..583
NoneNo IPR availableCDDcd059044CLcoord: 65..574
e-value: 0.0
score: 715.166
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 51..583
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 53..481
e-value: 1.5E-132
score: 444.3
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 230..241

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_5G030200.1CsaV3_5G030200.1mRNA