CsaV3_5G027730 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_5G027730
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionReverse transcriptase domain-containing protein
Locationchr5: 22848908 .. 22850702 (-)
RNA-Seq ExpressionCsaV3_5G027730
SyntenyCsaV3_5G027730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGCTTGACAAACGATAATAAACCACAAACCTCAAGCTCTAGTGCTCCTCAAGGAGCACATCAGTCAAATTTGAATCAAACAAGACCTATTTCTTGCTTCTTGTGTAAAGGCCCTCATCATGTAGCCGAATGCCCACACCGAGGCTCGTTTACAGCTTTTAAAGCCTTCTTACAGTGTTCTAACGATTCCGAGGCAAAAACAGAAGTTGAAAGGGAAGATGAGAGGGACACCCGTCGCATGGGTGCGCTAAAATTACTATCAACAATTCAAAATAAGGCATCTCACCAAAAGGATGCTTTAAAAAATGTACTCATGTTTGTCGACGCGGTCATAAACTCCAAACCCGAAAAGAGCACCATGGTAGACTCAGGTACAACTCATAATTTCATTTCTGAACAAGAAGCACATCAGTTGGAGTTGAAAATCGAGATAGACATAGGCAAGATGAAAGTTGTTAATTCTGAAGCCTTGTCAATAGTTGGAGTATCGAAAAAGATATCTATACATTTAGGCGAATGGAGAGAAGATGTTGATTTACTCATTGTTTGCATGGATGATTTTAATGTAGAACTCGGTATGGAGTTTCTCTTACAACATGAAGTCATTCCCATGCCACTGGCTTAGTGTAATGGTAGTAACGAGTAATCATCCGACAGTAGTTCAAGCAAATATCAAGCAACCAAGTGGGTGAGAATGATTTTAACCTTGAAATTGAATAGGGGCTCAATAGAGAAGAGTCGACCTTTATGGCCATCCAATTTTTGGATGAGCAGATGGAAATTGAGACTTCCCCAGTAGAGATTTAGAATGTTTTGAATGAATATGTTGATATCATGCCGCCTGAACTGCCAAAGTCATTACCTCCTCGACAGGGGATCGACCACGTGATTGAGCTTGTCCTAGGAGCCAAGCCCCCGACAAAGAATGCTTACCGAATGGCTCTACCTGAGCTAGTTGAGCTTAGGAAACAGTTAGATGAGTTATTGGTTGTCGGATTTTTCGGACTTGTGAAAGCACCTTATGAGGCCCCTGTACTGTTTCAGAAGAAGAAAGACGGAACACTTCGACTATGCACAGACTACCGAGCTTTAAACAAGGTTACAGTTCAGAACAAATATCTCATCCTGATCATAAATGACGTGTTTGACCAATTAAATGGAGCTCAGTACTTTTTGAAGCTCGACCTCCGGTCAAGCTAGTGCCCAGTTCGGATAGAACAAGGGAACGAACCGAAGACTGTGTGTAACAAGGTATGGAGCCTTCGAATTCCTCGTAATGCCCTTTGGCTTGACAAACTCCCCCGCAACCTCTTGCACTTTGATGAATCAGGTATTCCACAAGTACACAGATCAGTTTGTGGTGGTTGATCTCGATGACATTGTATTTTTCAGTTCTAGCCTTGAAGAACACCAGGTCCATATTTGGTTTGTCTTTGACAAGCTCTGGTGGAATCAATTATATGTTAAGAAAGAGAAATGTGCTTTTGCTCAACAGCGCATCAATTTTCTCGATCATGTCATCGAATGCGGAAAGATTCAAATGGACGAAGATAAGTTGCAAGCTATTAAGGAGTGGAGAGCCCCCACTTCTGTGACAGAGTTACGTTCCTTCCTTTGATTGGCTAATTACTATCGCCAGTTCATCGAAGGATTCTCAAGAAGAGCCGCACCGTTGACTGAGTTGTTGAAGAAAGATAAGACTTGGAGATGGCCACTAGAATGTCAAACTGGTTTTGACGAATTAAAGGTGACATTGACTAGAGGTCCTGTCCTCATATTGGTCATTTGTAGTTGA

mRNA sequence

AGCTTGACAAACGATAATAAACCACAAACCTCAAGCTCTAGTGCTCCTCAAGGAGCACATCAGTCAAATTTGAATCAAACAAGACCTATTTCTTGCTTCTTGTGTAAAGGCCCTCATCATGTAGCCGAATGCCCACACCGAGGCTCGTTTACAGCTTTTAAAGCCTTCTTACAGTGTTCTAACGATTCCGAGGCAAAAACAGAAGTTGAAAGGGAAGATGAGAGGGACACCCGTCGCATGGGTGCGCTAAAATTACTATCAACAATTCAAAATAAGGCATCTCACCAAAAGGATGCTTTAAAAAATAATGTTTTGAATGAATATGTTGATATCATGCCGCCTGAACTGCCAAAGTCATTACCTCCTCGACAGGGGATCGACCACTTCATCGAAGGATTCTCAAGAAGAGCCGCACCGTTGACTGAGTTGTTGAAGAAAGATAAGACTTGGAGATGGCCACTAGAATGTCAAACTGGTTTTGACGAATTAAAGGTGACATTGACTAGAGGTCCTGTCCTCATATTGGTCATTTGTAGTTG

Coding sequence (CDS)

AGCTTGACAAACGATAATAAACCACAAACCTCAAGCTCTAGTGCTCCTCAAGGAGCACATCAGTCAAATTTGAATCAAACAAGACCTATTTCTTGCTTCTTGTGTAAAGGCCCTCATCATGTAGCCGAATGCCCACACCGAGGCTCGTTTACAGCTTTTAAAGCCTTCTTACAGTGTTCTAACGATTCCGAGGCAAAAACAGAAGTTGAAAGGGAAGATGAGAGGGACACCCGTCGCATGGGTGCGCTAAAATTACTATCAACAATTCAAAATAAGGCATCTCACCAAAAGGATGCTTTAAAAAATAATGTTTTGAATGAATATGTTGATATCATGCCGCCTGAACTGCCAAAGTCATTACCTCCTCGACAGGGGATCGACCACTTCATCGAAGGATTCTCAAGAAGAGCCGCACCGTTGACTGAGTTGTTGAAGAAAGATAAGACTTGGAGATGGCCACTAGAATGTCAAACTGGTTTTGACGAATTAAAGGTGACATTGACTAGAGGTCCTGTCCTCATATTGGTCATTTGTAGTTG

Protein sequence

SLTNDNKPQTSSSSAPQGAHQSNLNQTRPISCFLCKGPHHVAECPHRGSFTAFKAFLQCSNDSEAKTEVEREDERDTRRMGALKLLSTIQNKASHQKDALKNNVLNEYVDIMPPELPKSLPPRQGIDHFIEGFSRRAAPLTELLKKDKTWRWPLECQTGFDELKVTLTRGPVLILVICSX
Homology
BLAST of CsaV3_5G027730 vs. NCBI nr
Match: KAE8648577.1 (hypothetical protein Csa_023676, partial [Cucumis sativus])

HSP 1 Score: 367.5 bits (942), Expect = 6.7e-98
Identity = 179/179 (100.00%), Postives = 179/179 (100.00%), Query Frame = 0

Query: 1   SLTNDNKPQTSSSSAPQGAHQSNLNQTRPISCFLCKGPHHVAECPHRGSFTAFKAFLQCS 60
           SLTNDNKPQTSSSSAPQGAHQSNLNQTRPISCFLCKGPHHVAECPHRGSFTAFKAFLQCS
Sbjct: 1   SLTNDNKPQTSSSSAPQGAHQSNLNQTRPISCFLCKGPHHVAECPHRGSFTAFKAFLQCS 60

Query: 61  NDSEAKTEVEREDERDTRRMGALKLLSTIQNKASHQKDALKNNVLNEYVDIMPPELPKSL 120
           NDSEAKTEVEREDERDTRRMGALKLLSTIQNKASHQKDALKNNVLNEYVDIMPPELPKSL
Sbjct: 61  NDSEAKTEVEREDERDTRRMGALKLLSTIQNKASHQKDALKNNVLNEYVDIMPPELPKSL 120

Query: 121 PPRQGIDHFIEGFSRRAAPLTELLKKDKTWRWPLECQTGFDELKVTLTRGPVLILVICS 180
           PPRQGIDHFIEGFSRRAAPLTELLKKDKTWRWPLECQTGFDELKVTLTRGPVLILVICS
Sbjct: 121 PPRQGIDHFIEGFSRRAAPLTELLKKDKTWRWPLECQTGFDELKVTLTRGPVLILVICS 179

BLAST of CsaV3_5G027730 vs. NCBI nr
Match: KAA0046120.1 (uncharacterized protein E6C27_scaffold157G00530 [Cucumis melo var. makuwa])

HSP 1 Score: 141.0 bits (354), Expect = 1.0e-29
Identity = 72/105 (68.57%), Postives = 82/105 (78.10%), Query Frame = 0

Query: 1   SLTNDNKPQTSSSSAPQGAHQSNLNQTRPISCFLCKGPHHVAECPHRGSFTAFKAFLQCS 60
           + TN+ KPQTSSSS  QGAHQSNL+QTRPISCFLCKGPH VA+C HRGS  A KA LQCS
Sbjct: 114 NFTNEKKPQTSSSSTLQGAHQSNLSQTRPISCFLCKGPHWVAKCSHRGSLIASKASLQCS 173

Query: 61  NDSEAKTEVEREDERDTRRMGALKLLSTIQNKASHQKDALKNNVL 106
           N+ +AK E +REDE DT RMGALK L  IQ  ASHQKDA +  ++
Sbjct: 174 NNLKAKIEGKREDEGDTPRMGALKFLLAIQKNASHQKDAFEKGLM 218

BLAST of CsaV3_5G027730 vs. NCBI nr
Match: KAA0043593.1 (RNA-directed DNA polymerase-like protein [Cucumis melo var. makuwa] >TYK29767.1 RNA-directed DNA polymerase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 122.9 bits (307), Expect = 2.9e-24
Identity = 66/114 (57.89%), Postives = 76/114 (66.67%), Query Frame = 0

Query: 36  KGPHHVAECPHRGSFTAFKAFLQCSNDSEAKTEVEREDERDTRRMGALKLLSTIQNKASH 95
           K PH +AECPHRGS TA +A LQC N+ E +TE+ERE   D  +MG LK+LS IQ KAS+
Sbjct: 139 KVPHRIAECPHRGSLTALQASLQCYNNLEVETEMERE---DAPQMGVLKILSAIQKKASY 198

Query: 96  QKDALKN------------------NVLNEYVDIMPPELPKSLPPRQGIDHFIE 132
           QKDA +                    VLNEYVDIMPPELPKSLPPR+GIDH IE
Sbjct: 199 QKDAFEKGLMFVDAVINSKPAKSTMKVLNEYVDIMPPELPKSLPPRRGIDHKIE 249

BLAST of CsaV3_5G027730 vs. NCBI nr
Match: TYK19296.1 (uncharacterized protein E5676_scaffold295G00130 [Cucumis melo var. makuwa])

HSP 1 Score: 98.6 bits (244), Expect = 5.8e-17
Identity = 65/150 (43.33%), Postives = 76/150 (50.67%), Query Frame = 0

Query: 1   SLTNDNKPQTSSSSAPQGAHQSNLNQTRPISCFLCKGPHHVAECPHRGSFTAFKAFLQCS 60
           + TND KPQ SSS  PQGAH +N N T+PI CFLCKGP                      
Sbjct: 154 NFTNDKKPQISSSKIPQGAHPTNSNLTKPIFCFLCKGP---------------------- 213

Query: 61  NDSEAKTEVEREDERDTRRMGALKLLSTIQN----KASHQKDALKNNVLNE-------YV 120
              +A+TEVEREDE DT R+         Q     +   +KD  K   +N        YV
Sbjct: 214 --LQAETEVEREDEGDTPRISTTHNFIFEQEACRLEFKIEKDICKMKAVNSKALPIVGYV 273

Query: 121 DIMPPELPKSLPPRQGIDHFIEGFSRRAAP 140
           DIM PELPK+LPPR+GIDH IE   R   P
Sbjct: 274 DIMSPELPKTLPPRRGIDHEIELVPRTKPP 279

BLAST of CsaV3_5G027730 vs. NCBI nr
Match: KAA0059226.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 98.6 bits (244), Expect = 5.8e-17
Identity = 65/150 (43.33%), Postives = 76/150 (50.67%), Query Frame = 0

Query: 1    SLTNDNKPQTSSSSAPQGAHQSNLNQTRPISCFLCKGPHHVAECPHRGSFTAFKAFLQCS 60
            + TND KPQ SSS  PQGAH +N N T+PI CFLCKGP                      
Sbjct: 1268 NFTNDKKPQISSSKIPQGAHPTNSNLTKPIFCFLCKGP---------------------- 1327

Query: 61   NDSEAKTEVEREDERDTRRMGALKLLSTIQN----KASHQKDALKNNVLNE-------YV 120
               +A+TEVEREDE DT R+         Q     +   +KD  K   +N        YV
Sbjct: 1328 --LQAETEVEREDEGDTPRISTTHNFIFEQEACRLEFKIEKDICKMKAVNSKALPIVGYV 1387

Query: 121  DIMPPELPKSLPPRQGIDHFIEGFSRRAAP 140
            DIM PELPK+LPPR+GIDH IE   R   P
Sbjct: 1388 DIMSPELPKTLPPRRGIDHEIELVPRTKPP 1393

BLAST of CsaV3_5G027730 vs. ExPASy TrEMBL
Match: A0A5A7TSJ2 (RT_RNaseH_2 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold157G00530 PE=4 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 5.0e-30
Identity = 72/105 (68.57%), Postives = 82/105 (78.10%), Query Frame = 0

Query: 1   SLTNDNKPQTSSSSAPQGAHQSNLNQTRPISCFLCKGPHHVAECPHRGSFTAFKAFLQCS 60
           + TN+ KPQTSSSS  QGAHQSNL+QTRPISCFLCKGPH VA+C HRGS  A KA LQCS
Sbjct: 114 NFTNEKKPQTSSSSTLQGAHQSNLSQTRPISCFLCKGPHWVAKCSHRGSLIASKASLQCS 173

Query: 61  NDSEAKTEVEREDERDTRRMGALKLLSTIQNKASHQKDALKNNVL 106
           N+ +AK E +REDE DT RMGALK L  IQ  ASHQKDA +  ++
Sbjct: 174 NNLKAKIEGKREDEGDTPRMGALKFLLAIQKNASHQKDAFEKGLM 218

BLAST of CsaV3_5G027730 vs. ExPASy TrEMBL
Match: A0A5D3E0Z4 (RNA-directed DNA polymerase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2208G00240 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 1.4e-24
Identity = 66/114 (57.89%), Postives = 76/114 (66.67%), Query Frame = 0

Query: 36  KGPHHVAECPHRGSFTAFKAFLQCSNDSEAKTEVEREDERDTRRMGALKLLSTIQNKASH 95
           K PH +AECPHRGS TA +A LQC N+ E +TE+ERE   D  +MG LK+LS IQ KAS+
Sbjct: 139 KVPHRIAECPHRGSLTALQASLQCYNNLEVETEMERE---DAPQMGVLKILSAIQKKASY 198

Query: 96  QKDALKN------------------NVLNEYVDIMPPELPKSLPPRQGIDHFIE 132
           QKDA +                    VLNEYVDIMPPELPKSLPPR+GIDH IE
Sbjct: 199 QKDAFEKGLMFVDAVINSKPAKSTMKVLNEYVDIMPPELPKSLPPRRGIDHKIE 249

BLAST of CsaV3_5G027730 vs. ExPASy TrEMBL
Match: A0A5A7UYE8 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold430G001570 PE=4 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 2.8e-17
Identity = 65/150 (43.33%), Postives = 76/150 (50.67%), Query Frame = 0

Query: 1    SLTNDNKPQTSSSSAPQGAHQSNLNQTRPISCFLCKGPHHVAECPHRGSFTAFKAFLQCS 60
            + TND KPQ SSS  PQGAH +N N T+PI CFLCKGP                      
Sbjct: 1268 NFTNDKKPQISSSKIPQGAHPTNSNLTKPIFCFLCKGP---------------------- 1327

Query: 61   NDSEAKTEVEREDERDTRRMGALKLLSTIQN----KASHQKDALKNNVLNE-------YV 120
               +A+TEVEREDE DT R+         Q     +   +KD  K   +N        YV
Sbjct: 1328 --LQAETEVEREDEGDTPRISTTHNFIFEQEACRLEFKIEKDICKMKAVNSKALPIVGYV 1387

Query: 121  DIMPPELPKSLPPRQGIDHFIEGFSRRAAP 140
            DIM PELPK+LPPR+GIDH IE   R   P
Sbjct: 1388 DIMSPELPKTLPPRRGIDHEIELVPRTKPP 1393

BLAST of CsaV3_5G027730 vs. ExPASy TrEMBL
Match: A0A5D3D6V5 (Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold295G00130 PE=4 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 2.8e-17
Identity = 65/150 (43.33%), Postives = 76/150 (50.67%), Query Frame = 0

Query: 1   SLTNDNKPQTSSSSAPQGAHQSNLNQTRPISCFLCKGPHHVAECPHRGSFTAFKAFLQCS 60
           + TND KPQ SSS  PQGAH +N N T+PI CFLCKGP                      
Sbjct: 154 NFTNDKKPQISSSKIPQGAHPTNSNLTKPIFCFLCKGP---------------------- 213

Query: 61  NDSEAKTEVEREDERDTRRMGALKLLSTIQN----KASHQKDALKNNVLNE-------YV 120
              +A+TEVEREDE DT R+         Q     +   +KD  K   +N        YV
Sbjct: 214 --LQAETEVEREDEGDTPRISTTHNFIFEQEACRLEFKIEKDICKMKAVNSKALPIVGYV 273

Query: 121 DIMPPELPKSLPPRQGIDHFIEGFSRRAAP 140
           DIM PELPK+LPPR+GIDH IE   R   P
Sbjct: 274 DIMSPELPKTLPPRRGIDHEIELVPRTKPP 279

BLAST of CsaV3_5G027730 vs. ExPASy TrEMBL
Match: A0A6J1IEF9 (uncharacterized protein LOC111474945 OS=Cucurbita maxima OX=3661 GN=LOC111474945 PE=4 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 5.3e-16
Identity = 59/204 (28.92%), Postives = 85/204 (41.67%), Query Frame = 0

Query: 5   DNKPQTSSSSAPQGAHQSNLNQTRPISCFLCKGPHHVAECPHRGSFTAFKAFLQCSNDSE 64
           D  PQ + +   +G +    + T P+ C LCKGPH V+ CPHR S TA +  +Q SND++
Sbjct: 369 DRPPQNNQAGTSRGPYHQRNHPTTPLQCMLCKGPHKVSYCPHRASLTALQVSIQESNDAK 428

Query: 65  AKTEVEREDERDTRRMGALKLLSTIQNKASHQKDALK----------------------- 124
            +T +++++++D  RMGALK LS +Q K   ++   K                       
Sbjct: 429 VETMLDKKEDQDNPRMGALKFLSALQRKVEPKEIVEKGLIVPFKIGDWTGELDLVVARMD 488

Query: 125 ------------------------------------------------------NNVLNE 132
                                                                 N VLN+
Sbjct: 489 DFDVVLGMEFLLEHKVIPMPLAKCLVITDRNPTVIPASIKQPVTTTEETVPNEINEVLND 548

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAE8648577.16.7e-98100.00hypothetical protein Csa_023676, partial [Cucumis sativus][more]
KAA0046120.11.0e-2968.57uncharacterized protein E6C27_scaffold157G00530 [Cucumis melo var. makuwa][more]
KAA0043593.12.9e-2457.89RNA-directed DNA polymerase-like protein [Cucumis melo var. makuwa] >TYK29767.1 ... [more]
TYK19296.15.8e-1743.33uncharacterized protein E5676_scaffold295G00130 [Cucumis melo var. makuwa][more]
KAA0059226.15.8e-1743.33gag/pol protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7TSJ25.0e-3068.57RT_RNaseH_2 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A5D3E0Z41.4e-2457.89RNA-directed DNA polymerase-like protein OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A5A7UYE82.8e-1743.33Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold430G0015... [more]
A0A5D3D6V52.8e-1743.33Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A6J1IEF95.3e-1628.92uncharacterized protein LOC111474945 OS=Cucurbita maxima OX=3661 GN=LOC111474945... [more]

Pages

Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR043128Reverse transcriptase/Diguanylate cyclase domainGENE3D3.30.70.270coord: 125..177
e-value: 5.4E-10
score: 41.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 127..175

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_5G027730.1CsaV3_5G027730.1mRNA