CsaV3_5G027370 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_5G027370
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Descriptionprotease Do-like 1, chloroplastic
Locationchr5: 22351226 .. 22357016 (-)
RNA-Seq ExpressionCsaV3_5G027370
SyntenyCsaV3_5G027370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCAAGCAAAAAGGATGCAAATAGATCAAGTCTTTATATATATAATAATCCATTGACTTGTTTAATAATTATATGTGAAATATAAAACATAATAAAGAAAAAAAGACTTTTATAGAGACCATATAAAAGACATGAGAAATACCTGTCATGTCCACCAATAACACAGTAAAGTTAAGAAGAAGAAGAAGAAGAAGAAGAACCATAGTGGATATTAATAAACCAAAATTTATTACAAAAATTAGTATTATCATAAACAGAAGAAACTGGTTGAAATTGGATTTCATTCATCGATTCAATTTTCAGCAACTGTCCAACAAACCCAAAACAAATGGCTGCTGCTGCTGCATTTTCTCTGCTGCCTTCTTCACTTTCTCGTCCTCCTAAAAGACCACCCACTTCCTTTTTTCTCTCAAAGTCCATTTCTTTTCACAATTTTTCCAATCCCACCCGCCATTTTAGATACCCCATCTTCTCTCTTCTTCACCACAATAAGAAACACTCTCAATTTTCTCCTTCAATTCTTCCAAAACTCCCCGTTCCCTCCAACCCCTTTGCTTCAATTTGCGAATCGCTTCTTGTTTTCTCCACTTCGGTGCTCTTGTCCTTTGCTCTTTTTGTAACAGATGTTGATCCGGCGGTTGCTTTTGTTGTCACTACGCCGAGGAAGTTGCAGACGGATGAGCTCGCTACAGTTCGTCTTTTTCAGGAGAATACTCCTTCTGTTGTTTATATCACGAATCTTGCTGTTAGGTAATTGTATTATTATACCCTTTTGGAGCTGACTTCTGATTACAGTTTTTTCTTTTTTTTCTCAATATAAGAAATATTTCATTGAAAGAATGATGTTAGCGCCTTAGCGGAGACCCTAGGCACCACTAATGATTTCCGATCAGAAACTAAGAAAGAGTGAAACTCTTGAGAGTGTGCCTGGTATTGCACCAGTACAACGCAGTAGATAGAACCAAATTCATTAAACTATCGAAACAAGTAGAGGAATTTCTAAAGAGATGCTTATTATGTTTGCCCCATAGTACGCAATATGGCCAACCAATGATTTTATGTAAATTTTAGGCAGGATGCTTTTACTTTAGACGTGCTGGAGGTACCACAGGGGTCCGGTTCTGGATTTGTGTGGGATAAGGATGGTCATATTGTCACCAATTATCATGTTATTCGTGGTGCATCTGATCTCAGGTTAGTTATGAACCCTTTTCCGTGGTTTGCAGTTTTTGGGTTTAATTTTGTTTAGACTAAGAGTTAATTTATTTGTCATTTTAGTATTTGGACCTTCCTCAGCTTGTATTGATTTGTATGTTCATTACCTTGGGGGCTTTATCCATGTAATGCTGTGTTATTACTTCTAGTATGTATAGCCTGTTCATACCCATTGAAGCATCATCGAAGCACTTAATTGTAGAACCACCATGTCGCAAATGGCCCTCAGAAAATGTTTTTAGGCTTTATATTTTCAGATATGTGCAGCCACATAGGCAGAACTGTATCATATTTTTGGCTATGTGATTTTCTTTTATAATAACCTCTGGAATTATCAGTAAATATTTTCGACCAAAACAAATATGAATTATTTGTCTTGGTTATGTGGTAATTCACACAGCCATACTTTTTTTGCTATGTGAATTATCAGGAAGGAGATATCAGGGCATTATCAGTAAATATATTTGTTTTTAAAATGCAGAAATTTTAAATTTTCCACCTTCCCTTGAGGTCAAAGGGGAAATTTTGGAACACTTTCAAGAACTCTACTCCAAATTTCTTGGCCTAAGACTGTCTTCAATGTTTGGATTGACAATTAGCTAATTAGTCCAGAAAACCATGGTTGTCGATTCCCCATAAAGACAAATTGGCTTTTTCTTTCTTCTGTGAGTGTTGGAGTTAGCTTGTTCGCTTCTTGGATATTCTCATATTCATAGGACAATTGCTTGGCCCTACTATATTTGGTTGTCGAGAAACTCATAGGATGTTAAGTCGTAAGTAAGTCACCATTGTTCAAACCCATTTCTTTGGAGGCTTTTTTTATTTTTTATTTTGATGCCATATATTGACCTTGAAGTCAGCCGGTGGTGGTTAGCCAAGTGGAGTTTCGATGTTTGTTTTGAGGGAAATTTGGAGTTTTATGAGTAAATTAGGCTTTCTCAAGTTTTATGTTTTTCCCAAATCAACTAGAGTGTTTTCTAAAACTCTTTTAGCCTCAGCAAAATGCAAAAGAAGGTACTATCAAGTTCTTTTATACTTACAATGAGCTAGAATTGGATTGACATTAGACTTAAGACTTAGCAGTGGCTCCTTATTATTCGCATTTAATCTGGTTTTAGTTATGTACAGAGTCACTCTAGCTGACCAAACTACTTTCGATGCGAAAGTTGTTGGATTTGACCAAGACAAGGATGTTGCTGTCTTGAGTATCGATGCACCTAAAGACAAACTAAGACCTATACCTGTTGGTATATCAGCAGATTTGCTTGTTGGTCAAAAGGTGTTTGCTATTGGAAACCCTGTAAGTTTTTCTATTCATTGCCCTTTTTCCCTGGGTGTATGTGCACATTATCATATCCATTCATCTTTTATATCAAAGTTCATGCTGTTGATTTCTTAAAACAAAAATGAAAAATCTAGGATGAAGCGATCTCTGGAAAGAGGATAATCTCTTAAAATGTTTTCAAAAGAGGAAAACCCCAGATTAATTTTGCGTTAAATGGACAATCCTTATATGCTATAGTTTTATCTACAAAATCAATCGGGGGAATGAACTCATTGTTTAGATTAACTAGGAAAAAAATTGTTTTAAAATTTTTGTACGTAAATCTTTTTTTTATAAAGACAACTATTTCAAAAGCTATTTTGAGTGGTTGTTAAACACTTCTATTTTTTCTAAAATGTCTTAGTTTTAAAATAAAATTTTTGAAATGTTAAACCAACCATAGTCTTAGTTGGGAGATGATCACCCCTTTTTTCATTTTCCCTGAAGCCAGTCTCTTCATTTGTTATCTTGTACATTTCCCAATTTGATGAACTAAAAATATAGTAATTTTCGTCCGTGCAGTTTGGACTGGATCATACTCTTACAACTGGTGTAATCAGGTATGAAACCCACAAGTTTTAATGGATTATATTCCTTACAAATTCTTTAGAAATTTACATTTTAATTTAGTATGTTAATCTTATACTATAATTGAGCCCCCAAAAAATTATTAGAGATTGTAATTTCAATTAACAATAATCACGAAGATGATGTTTGTTTTAGAGTTGAAATAATTCTGTTTATATATCTGTCTCCTTTTTACTTTTCCTAAAATTGTTTATTTTCTTCATGATCCTTCAAATGCAAATGGGTTTATCCTTATGAGACATGTTATATTTTTACTATTTGGTTGCCGTCGAAACCTGTATTCATAGGCTTTGAAACTAGGGGTGATCATCAATGGATCGACCAGCAGACAATAGTTGGGTTTCCAGAAAATTTCGAAATTTTTTGGAAATCCAACATTCTCACTGAATTGCCTCTCTTACTCCCTCTGACCGACCAACTTGGTCGGTCATCTTGGTTTTGCGGTCTATCATGCTCACTCTATTTGAAACTGTAATACTAAACATTCAACTTGCCCTTGACAGTGGCCTTCGGAGAGAAATTAGTTCTGCGGCTACTGGTAGGCCAATTCAAGATGTCATACAGACGGATGCTGCCATTAATCCAGGAAACAGTGGAGGGCCACTTCTTGATAGTTCGGGGAACCTTATTGGAATTAATACGGCAATATATTCTCCATCTGGGGCCTCCTCTGGTGTTGGATTTTCAATTCCTGTTGACACAGTTAGTACACATGACATATAATTAACAAATTGAAGTTCCCTTAGTTTGATATTTGAAGTACATGATCCATTAACATTCTGTCAATTTAGGTGAGTGGTATTGTTGATCAGTTGGTGCGATTTGGGAAGGTGACAAGACCAATTTTAGGAATCAAGTTTGCTCCCGATCAGTCTGTCGAACAGTTGGGTGTAAGCGGAGTGCTTGTCTTAGATGCTCCTGCTAATGGTCCTGCTGGGAAAGCTGTACTCATTTCCTCTCTTTTCCTATCTGCATTCATATTCTTATATTCCTATAGGTTTCAATATTCCATTATGTGTACAAGAACTTATGAACGTCCCCCAACCTTAATACTTCAGTTCAAGAAGATCTAATCGCTTGACATTATGCAGGGCCTGCTACCGACTAAGCGCGATGCTTATGGTAGACTTATATTGGGAGACATAATAACATCAGTGAATGGGAAAAAGGTCACAAATGGAAGCGATTTGTATAGAATTCTTGACCAATGTAAAGTTGGTGATAAGGTAGCCTTCATTTCCCTGTTTGATCTTTTTACTTCAGCTTGTTTGAACTTTTTAACTTTTGAAGAAAGGCATCATACTGCTTTTATTTTAAGATGTTGGGAAGTAATTTAAAACTTTGGTTCATGCTTGAGTGGATCCATAATAAAGTTAGTAAATAGGCTGGTTTGATTGAATCTGTTGGATCCACGCACTGTTTGCCATTGTGTGCCATTGAATTCTCTGTGTCACGTAGTCATTTTTTTTTCAAAGAACTAAGAAGGATTTAAGACATGTTTGGAGATTTTGAAGTAGTCAAAATCATTTTTGTCATGTTGAAAATCACTTACAAACGTGCCAAGTTAATATAATTTAATTTTTACATTTTAAAACCCGATTTTCATACCGGGTAAAAGTAAATATTTTATCAACCTTTCTTTCTTATAGAATTATCTATGGGGTGTTTCTTCCTATAGTTTTGAAGTTTCTTATTGTATAGTATCTTAAAAGTTGAAATATCTTTTCATGTAGCATCATCGATCATGTGCCTTTTCAAAATTCATTTCACTAAATATTATAATCCGCATTTATGCATCAGTGGGAATTATTAAATGGCTCTATCCACTTGAACTAATGAATATTCTTCAAAACTTTGAGTTATGAAAAAGACAGCCAGATCCCATGTGATAAAATCCCATTGCCATCATATATATTGCCTCATAATGCTGAACAGTAGCATAGCATGTGGCTTTGAGTGACATTACACAGCCACAAAACCTTTGAATCGGCCTTTTTTTACTTGGGACAAAGAAGAACGAAGTTTTAATCCTATTTTGTTCATTAACTCCCTGCTTTGTTTTATTTTGATCCTCAAACTTTCAAAAACCGTATTTTAACTCTTAAACTTTTAAGCATTCAATAAACAAGTTCCGATTACAAATATAACATTTAAACTCAAAATATTAACCGAGGTAACACATTTTCAAAGCATTTGTAGATATTGTATAGCAAACTTTAGATCCATCTCTATTACCAACAAGAGTTCTATCAACAATAGAGTTTATTACTTGTAGATTTTGTTATATTTACAATTCTTTGAACATGTTAGTGTGTGCTAAGTTGTTAACCCTAAAAGTTGTATGTAGTGGTGATGCTAAGAAAAGGAAATATTTGCATGAATCTGCGGTATAGCAGAACAGGGTATGATGATAAGTAACAATTTGTTTGATGATATAGGTAACTGTGGAGGTACTACGTGGTGATCACATGGAGAAAATTCCAGTAATTCTGGAGCCAAAGCCTGACGAATCTTGAGCCAAACATAGTTGGGGGGGTTTGTCTTGGTTTTTTCTTCTCTCTTTTTTGTACATTACATTAATCAATATTAACAAGCTGTTTGTAAATAGTCTATTCTGGCTGCCACTCTGGCCCTTTTCTCAACTAATCACACTTCAAAAGAAG

mRNA sequence

ATGGCTGCTGCTGCTGCATTTTCTCTGCTGCCTTCTTCACTTTCTCGTCCTCCTAAAAGACCACCCACTTCCTTTTTTCTCTCAAAGTCCATTTCTTTTCACAATTTTTCCAATCCCACCCGCCATTTTAGATACCCCATCTTCTCTCTTCTTCACCACAATAAGAAACACTCTCAATTTTCTCCTTCAATTCTTCCAAAACTCCCCGTTCCCTCCAACCCCTTTGCTTCAATTTGCGAATCGCTTCTTGTTTTCTCCACTTCGGTGCTCTTGTCCTTTGCTCTTTTTGTAACAGATGTTGATCCGGCGGTTGCTTTTGTTGTCACTACGCCGAGGAAGTTGCAGACGGATGAGCTCGCTACAGTTCGTCTTTTTCAGGAGAATACTCCTTCTGTTGTTTATATCACGAATCTTGCTGTTAGGCAGGATGCTTTTACTTTAGACGTGCTGGAGGTACCACAGGGGTCCGGTTCTGGATTTGTGTGGGATAAGGATGGTCATATTGTCACCAATTATCATGTTATTCGTGGTGCATCTGATCTCAGAGTCACTCTAGCTGACCAAACTACTTTCGATGCGAAAGTTGTTGGATTTGACCAAGACAAGGATGTTGCTGTCTTGAGTATCGATGCACCTAAAGACAAACTAAGACCTATACCTGTTGGTATATCAGCAGATTTGCTTGTTGGTCAAAAGGTGTTTGCTATTGGAAACCCTTTTGGACTGGATCATACTCTTACAACTGGTGTAATCAGTGGCCTTCGGAGAGAAATTAGTTCTGCGGCTACTGGTAGGCCAATTCAAGATGTCATACAGACGGATGCTGCCATTAATCCAGGAAACAGTGGAGGGCCACTTCTTGATAGTTCGGGGAACCTTATTGGAATTAATACGGCAATATATTCTCCATCTGGGGCCTCCTCTGGTGTTGGATTTTCAATTCCTGTTGACACAGTGAGTGGTATTGTTGATCAGTTGGTGCGATTTGGGAAGGTGACAAGACCAATTTTAGGAATCAAGTTTGCTCCCGATCAGTCTGTCGAACAGTTGGGTGTAAGCGGAGTGCTTGTCTTAGATGCTCCTGCTAATGGTCCTGCTGGGAAAGCTGGCCTGCTACCGACTAAGCGCGATGCTTATGGTAGACTTATATTGGGAGACATAATAACATCAGTGAATGGGAAAAAGGTCACAAATGGAAGCGATTTGTATAGAATTCTTGACCAATGTAAAGTTGGTGATAAGGTAACTGTGGAGGTACTACGTGGTGATCACATGGAGAAAATTCCAGTAATTCTGGAGCCAAAGCCTGACGAATCTTGA

Coding sequence (CDS)

ATGGCTGCTGCTGCTGCATTTTCTCTGCTGCCTTCTTCACTTTCTCGTCCTCCTAAAAGACCACCCACTTCCTTTTTTCTCTCAAAGTCCATTTCTTTTCACAATTTTTCCAATCCCACCCGCCATTTTAGATACCCCATCTTCTCTCTTCTTCACCACAATAAGAAACACTCTCAATTTTCTCCTTCAATTCTTCCAAAACTCCCCGTTCCCTCCAACCCCTTTGCTTCAATTTGCGAATCGCTTCTTGTTTTCTCCACTTCGGTGCTCTTGTCCTTTGCTCTTTTTGTAACAGATGTTGATCCGGCGGTTGCTTTTGTTGTCACTACGCCGAGGAAGTTGCAGACGGATGAGCTCGCTACAGTTCGTCTTTTTCAGGAGAATACTCCTTCTGTTGTTTATATCACGAATCTTGCTGTTAGGCAGGATGCTTTTACTTTAGACGTGCTGGAGGTACCACAGGGGTCCGGTTCTGGATTTGTGTGGGATAAGGATGGTCATATTGTCACCAATTATCATGTTATTCGTGGTGCATCTGATCTCAGAGTCACTCTAGCTGACCAAACTACTTTCGATGCGAAAGTTGTTGGATTTGACCAAGACAAGGATGTTGCTGTCTTGAGTATCGATGCACCTAAAGACAAACTAAGACCTATACCTGTTGGTATATCAGCAGATTTGCTTGTTGGTCAAAAGGTGTTTGCTATTGGAAACCCTTTTGGACTGGATCATACTCTTACAACTGGTGTAATCAGTGGCCTTCGGAGAGAAATTAGTTCTGCGGCTACTGGTAGGCCAATTCAAGATGTCATACAGACGGATGCTGCCATTAATCCAGGAAACAGTGGAGGGCCACTTCTTGATAGTTCGGGGAACCTTATTGGAATTAATACGGCAATATATTCTCCATCTGGGGCCTCCTCTGGTGTTGGATTTTCAATTCCTGTTGACACAGTGAGTGGTATTGTTGATCAGTTGGTGCGATTTGGGAAGGTGACAAGACCAATTTTAGGAATCAAGTTTGCTCCCGATCAGTCTGTCGAACAGTTGGGTGTAAGCGGAGTGCTTGTCTTAGATGCTCCTGCTAATGGTCCTGCTGGGAAAGCTGGCCTGCTACCGACTAAGCGCGATGCTTATGGTAGACTTATATTGGGAGACATAATAACATCAGTGAATGGGAAAAAGGTCACAAATGGAAGCGATTTGTATAGAATTCTTGACCAATGTAAAGTTGGTGATAAGGTAACTGTGGAGGTACTACGTGGTGATCACATGGAGAAAATTCCAGTAATTCTGGAGCCAAAGCCTGACGAATCTTGA

Protein sequence

MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQFSPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELATVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASDLRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAIYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVLRGDHMEKIPVILEPKPDES*
Homology
BLAST of CsaV3_5G027370 vs. NCBI nr
Match: XP_004142804.1 (protease Do-like 1, chloroplastic [Cucumis sativus] >KGN51390.1 hypothetical protein Csa_008466 [Cucumis sativus])

HSP 1 Score: 852.4 bits (2201), Expect = 1.7e-243
Identity = 439/439 (100.00%), Postives = 439/439 (100.00%), Query Frame = 0

Query: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQF 60
           MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQF
Sbjct: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQF 60

Query: 61  SPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120
           SPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA
Sbjct: 61  SPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120

Query: 121 TVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD 180
           TVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD
Sbjct: 121 TVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD 180

Query: 181 LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240
           LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF
Sbjct: 181 LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240

Query: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300
           GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI
Sbjct: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300

Query: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360
           YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA
Sbjct: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360

Query: 361 PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 420
           PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL
Sbjct: 361 PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 420

Query: 421 RGDHMEKIPVILEPKPDES 440
           RGDHMEKIPVILEPKPDES
Sbjct: 421 RGDHMEKIPVILEPKPDES 439

BLAST of CsaV3_5G027370 vs. NCBI nr
Match: XP_008458761.1 (PREDICTED: protease Do-like 1, chloroplastic isoform X1 [Cucumis melo] >KAA0037525.1 protease Do-like 1 [Cucumis melo var. makuwa] >TYK02000.1 protease Do-like 1 [Cucumis melo var. makuwa])

HSP 1 Score: 833.9 bits (2153), Expect = 6.2e-238
Identity = 430/439 (97.95%), Postives = 432/439 (98.41%), Query Frame = 0

Query: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQF 60
           MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNP RHFRYPIFSLLH NKKHSQ 
Sbjct: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPARHFRYPIFSLLHQNKKHSQI 60

Query: 61  SPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120
           SPSI  KLP+PSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA
Sbjct: 61  SPSIHLKLPIPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120

Query: 121 TVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD 180
           TVRLFQENTPSVVYITNLA RQDAFTLDVLEVPQGSGSGFVWDKDGH+VTNYHVIRGASD
Sbjct: 121 TVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHVVTNYHVIRGASD 180

Query: 181 LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240
           LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF
Sbjct: 181 LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240

Query: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300
           GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI
Sbjct: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300

Query: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360
           YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA
Sbjct: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360

Query: 361 PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 420
           PANGPAGKAGL PTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL
Sbjct: 361 PANGPAGKAGLKPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 420

Query: 421 RGDHMEKIPVILEPKPDES 440
           RGDHMEKIPVILEPKPDES
Sbjct: 421 RGDHMEKIPVILEPKPDES 439

BLAST of CsaV3_5G027370 vs. NCBI nr
Match: XP_038890591.1 (protease Do-like 1, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 796.2 bits (2055), Expect = 1.4e-226
Identity = 416/439 (94.76%), Postives = 420/439 (95.67%), Query Frame = 0

Query: 4   AAAFSLLPSS---LSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQF 63
           A  FSL  S+   LSRPP R PTSFFLSKSISFHNFSNPTRHFRYPI SLL HNKK SQ 
Sbjct: 2   ATPFSLASSTFSPLSRPPNRTPTSFFLSKSISFHNFSNPTRHFRYPILSLLPHNKKLSQI 61

Query: 64  SPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 123
           S SI PKLP+ SNPFASI ESLL+FSTSVLLSF+LFVTDVDPAVAFVVTTPRKLQTDELA
Sbjct: 62  SASIHPKLPISSNPFASIWESLLIFSTSVLLSFSLFVTDVDPAVAFVVTTPRKLQTDELA 121

Query: 124 TVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD 183
           TVRLFQENTPSVVYITNLA RQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD
Sbjct: 122 TVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD 181

Query: 184 LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 243
           LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF
Sbjct: 182 LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 241

Query: 244 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 303
           GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI
Sbjct: 242 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 301

Query: 304 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 363
           YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA
Sbjct: 302 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 361

Query: 364 PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 423
           PANGPAGKAGL PTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL
Sbjct: 362 PANGPAGKAGLQPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 421

Query: 424 RGDHMEKIPVILEPKPDES 440
           RGDHMEKIPVILEPKPDES
Sbjct: 422 RGDHMEKIPVILEPKPDES 440

BLAST of CsaV3_5G027370 vs. NCBI nr
Match: XP_023515810.1 (protease Do-like 1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 773.5 bits (1996), Expect = 1.0e-219
Identity = 404/441 (91.61%), Postives = 413/441 (93.65%), Query Frame = 0

Query: 4   AAAFSLLP-----SSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHS 63
           AAAFSL       S LSRPP   PTSFFLSKSI FHNFSNP+ HFRYPIFS+L HNKK +
Sbjct: 2   AAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSCHFRYPIFSILGHNKKRN 61

Query: 64  QFSPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDE 123
           Q S +   KL  PSNPFASICESLL+F+TSV LSF+LFVTDVDPA AFVVTTPRKLQTDE
Sbjct: 62  QISATTDTKLTNPSNPFASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTDE 121

Query: 124 LATVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGA 183
           LATVRLFQENTPSVVYITNLA RQDAFTLDVLEVPQGSGSGFVWDKDG+IVTNYHVIRGA
Sbjct: 122 LATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRGA 181

Query: 184 SDLRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGN 243
           SDLRVTLADQTTFDAKVVGFDQDKDVAVL IDAPKDKLRPIPVGISADLLVGQKVFAIGN
Sbjct: 182 SDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIGN 241

Query: 244 PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT 303
           PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT
Sbjct: 242 PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT 301

Query: 304 AIYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVL 363
           AIYSPSGASSGVGFSIPVDTVSGIVDQLVR+GKVTRPILGIKFAPDQSVEQLGVSGVLVL
Sbjct: 302 AIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLVL 361

Query: 364 DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVE 423
           DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKV NGSDLYRILDQCKVGDKV VE
Sbjct: 362 DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIVE 421

Query: 424 VLRGDHMEKIPVILEPKPDES 440
           VLRGDHMEKIPVILEPKPDES
Sbjct: 422 VLRGDHMEKIPVILEPKPDES 442

BLAST of CsaV3_5G027370 vs. NCBI nr
Match: XP_022922573.1 (protease Do-like 1, chloroplastic [Cucurbita moschata] >KAG6602439.1 Protease Do-like 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 772.3 bits (1993), Expect = 2.2e-219
Identity = 404/441 (91.61%), Postives = 412/441 (93.42%), Query Frame = 0

Query: 4   AAAFSLLP-----SSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHS 63
           AAAFSL       S LSRPP   PTSFFLSKSI FHNFSNP+ HFRYPIFSLL HNKK +
Sbjct: 2   AAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKRN 61

Query: 64  QFSPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDE 123
           Q S +   KL  PSNP ASICESLL+F+TSV LSF+LFVTDVDPA AFVVTTPRKLQTDE
Sbjct: 62  QISATTDTKLTNPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTDE 121

Query: 124 LATVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGA 183
           LATVRLFQENTPSVVYITNLA RQDAFTLDVLEVPQGSGSGFVWDKDG+IVTNYHVIRGA
Sbjct: 122 LATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRGA 181

Query: 184 SDLRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGN 243
           SDLRVTLADQTTFDAKVVGFDQDKDVAVL IDAPKDKLRPIPVGISADLLVGQKVFAIGN
Sbjct: 182 SDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIGN 241

Query: 244 PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT 303
           PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT
Sbjct: 242 PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT 301

Query: 304 AIYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVL 363
           AIYSPSGASSGVGFSIPVDTVSGIVDQLVR+GKVTRPILGIKFAPDQSVEQLGVSGVLVL
Sbjct: 302 AIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLVL 361

Query: 364 DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVE 423
           DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKV NGSDLYRILDQCKVGDKV VE
Sbjct: 362 DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIVE 421

Query: 424 VLRGDHMEKIPVILEPKPDES 440
           VLRGDHMEKIPVILEPKPDES
Sbjct: 422 VLRGDHMEKIPVILEPKPDES 442

BLAST of CsaV3_5G027370 vs. ExPASy Swiss-Prot
Match: O22609 (Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 SV=2)

HSP 1 Score: 632.5 bits (1630), Expect = 3.6e-180
Identity = 353/446 (79.15%), Postives = 368/446 (82.51%), Query Frame = 0

Query: 1   MAAAAAFSLLPSS---LSRPPKRPPTSFFLSKSISFHNFSNPTRHFRY-PIFSLLHHNKK 60
           MA   + SLL SS   L  PP    + F LS S S      P R  RY  I S L  N  
Sbjct: 1   MATTTSCSLLLSSTLFLHSPPSSHLSFFNLSSSRSSPISLYPIRSKRYFRILSKLSLNDN 60

Query: 61  HSQFSPSILPKLPVPS-NPFASICESLLVFSTSVLLSFALFVTD--VDPAVAFVVTTPRK 120
           +       L   P  +  PF  +C       TSV LSF+LF     V+ A AFVV+TP+K
Sbjct: 61  NRDDDDDTLHFTPFSAVKPFFLLC-------TSVALSFSLFAASPAVESASAFVVSTPKK 120

Query: 121 LQTDELATVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYH 180
           LQTDELATVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDK GHIVTNYH
Sbjct: 121 LQTDELATVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKQGHIVTNYH 180

Query: 181 VIRGASDLRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKV 240
           VIRGASDLRVTLADQTTFDAKVVGFDQDKDVAVL IDAPK+KLRPIPVG+SADLLVGQKV
Sbjct: 181 VIRGASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKNKLRPIPVGVSADLLVGQKV 240

Query: 241 FAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNL 300
           FAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSG L
Sbjct: 241 FAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGTL 300

Query: 301 IGINTAIYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVS 360
           IGINTAIYSPSGASSGVGFSIPVDTV GIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVS
Sbjct: 301 IGINTAIYSPSGASSGVGFSIPVDTVGGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVS 360

Query: 361 GVLVLDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGD 420
           GVLVLDAP +GPAGKAGL  TKRD YGRL+LGDIITSVNG KV+NGSDLYRILDQCKVGD
Sbjct: 361 GVLVLDAPPSGPAGKAGLQSTKRDGYGRLVLGDIITSVNGTKVSNGSDLYRILDQCKVGD 420

Query: 421 KVTVEVLRGDHMEKIPVILEPKPDES 440
           +VTVEVLRGDH EKI V LEPKPDES
Sbjct: 421 EVTVEVLRGDHKEKISVTLEPKPDES 439

BLAST of CsaV3_5G027370 vs. ExPASy Swiss-Prot
Match: Q9LU10 (Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.7e-81
Identity = 179/355 (50.42%), Postives = 226/355 (63.66%), Query Frame = 0

Query: 101 DPAVAFV------VTTPRKLQTDELATVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQ 160
           DP+VA V      V     L   E   V+LF++NT SVV I ++ +R       V+E+P+
Sbjct: 93  DPSVATVEDVSPTVFPAGPLFPTEGRIVQLFEKNTYSVVNIFDVTLRPQLKMTGVVEIPE 152

Query: 161 GSGSGFVWDKDGHIVTNYHVIRGASDLRVTLAD-------------QTTFDAKVVGFDQD 220
           G+GSG VWD  G+IVTNYHVI  A     +  D             Q  F+ K+VG D+ 
Sbjct: 153 GNGSGVVWDGQGYIVTNYHVIGNALSRNPSPGDVVGRVNILASDGVQKNFEGKLVGADRA 212

Query: 221 KDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSA 280
           KD+AVL +DAP+  L+PI VG S  L VGQ+  AIGNPFG DHTLT GVISGL R+I S 
Sbjct: 213 KDLAVLKVDAPETLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFS- 272

Query: 281 ATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAIYSPSGASSGVGFSIPVDTVSG 340
            TG  I   IQTDAAINPGNSGGPLLDS GNLIGINTAI++ +G S+GVGF+IP  TV  
Sbjct: 273 QTGVTIGGGIQTDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVLK 332

Query: 341 IVDQLVRFGKVTRPILGIKFAPDQSVEQLGV-SGVLVLDAPANGPAGKAGLLPTKRDAYG 400
           IV QL++F KV R  + I+ APD    QL V +G LVL  P    A KAGL PT R   G
Sbjct: 333 IVPQLIQFSKVLRAGINIELAPDPVANQLNVRNGALVLQVPGKSLAEKAGLHPTSRGFAG 392

Query: 401 RLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVLRGDHMEKIPVILEPK 436
            ++LGDII +V+ K V N ++L +ILD+  VGDKVT+++ RG+   ++ + LE K
Sbjct: 393 NIVLGDIIVAVDDKPVKNKAELMKILDEYSVGDKVTLKIKRGNEDLELKISLEEK 446

BLAST of CsaV3_5G027370 vs. ExPASy Swiss-Prot
Match: Q2YMX6 (Probable periplasmic serine endoprotease DegP-like OS=Brucella abortus (strain 2308) OX=359391 GN=htrA PE=3 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 3.7e-52
Identity = 121/293 (41.30%), Postives = 166/293 (56.66%), Query Frame = 0

Query: 151 EVPQGSGSGFVWDKDGHIVTNYHVIRGASDLRVTLADQTTFDAKVVGFDQDKDVAVLSID 210
           E P   GSGFV  +DG++VTN HV+       V L D T  DAK++G D   D+AVL I+
Sbjct: 130 ERPVAQGSGFVISEDGYVVTNNHVVSDGDAYTVVLDDGTELDAKLIGADPRTDLAVLKIN 189

Query: 211 APKDKLRPIPVGISADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDV 270
           APK K   +  G    + VG  V A+GNPFGL  T+T+G++S   R+I +     P  D 
Sbjct: 190 APKRKFVYVAFGDDNKVRVGDWVVAVGNPFGLGGTVTSGIVSARGRDIGAG----PYDDF 249

Query: 271 IQTDAAINPGNSGGPLLDSSGNLIGINTAIYSPSGASSGVGFSIPVDTVSGIVDQLVRFG 330
           IQ DAA+N GNSGGP  D SG +IGINTAI+SPSG S G+ F+IP  T   +VDQL++ G
Sbjct: 250 IQIDAAVNKGNSGGPAFDLSGEVIGINTAIFSPSGGSVGIAFAIPSSTAKQVVDQLIKKG 309

Query: 331 KVTRPILGIKFAP--DQSVEQLGVS---GVLVLDAPANGPAGKAGLLPTKRDAYGRLILG 390
            V R  +G++  P        LG++   G +V     +GPA KAG+             G
Sbjct: 310 SVERGWIGVQIQPVTKDIAASLGLAEEKGAIVASPQDDGPAAKAGIK-----------AG 369

Query: 391 DIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVLRGDHMEKIPVILEPKPDE 439
           D+IT+VNG+ V +  DL R +     G+K  + V R +  E+I V +   P++
Sbjct: 370 DVITAVNGETVQDPRDLARKVANIAPGEKAALTVWRKNKAEEINVTIAAMPND 407

BLAST of CsaV3_5G027370 vs. ExPASy Swiss-Prot
Match: P0C114 (Probable periplasmic serine endoprotease DegP-like OS=Brucella abortus biovar 1 (strain 9-941) OX=262698 GN=htrA PE=3 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 3.7e-52
Identity = 121/293 (41.30%), Postives = 166/293 (56.66%), Query Frame = 0

Query: 151 EVPQGSGSGFVWDKDGHIVTNYHVIRGASDLRVTLADQTTFDAKVVGFDQDKDVAVLSID 210
           E P   GSGFV  +DG++VTN HV+       V L D T  DAK++G D   D+AVL I+
Sbjct: 130 ERPVAQGSGFVISEDGYVVTNNHVVSDGDAYTVVLDDGTELDAKLIGADPRTDLAVLKIN 189

Query: 211 APKDKLRPIPVGISADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDV 270
           APK K   +  G    + VG  V A+GNPFGL  T+T+G++S   R+I +     P  D 
Sbjct: 190 APKRKFVYVAFGDDNKVRVGDWVVAVGNPFGLGGTVTSGIVSARGRDIGAG----PYDDF 249

Query: 271 IQTDAAINPGNSGGPLLDSSGNLIGINTAIYSPSGASSGVGFSIPVDTVSGIVDQLVRFG 330
           IQ DAA+N GNSGGP  D SG +IGINTAI+SPSG S G+ F+IP  T   +VDQL++ G
Sbjct: 250 IQIDAAVNKGNSGGPAFDLSGEVIGINTAIFSPSGGSVGIAFAIPSSTAKQVVDQLIKKG 309

Query: 331 KVTRPILGIKFAP--DQSVEQLGVS---GVLVLDAPANGPAGKAGLLPTKRDAYGRLILG 390
            V R  +G++  P        LG++   G +V     +GPA KAG+             G
Sbjct: 310 SVERGWIGVQIQPVTKDIAASLGLAEEKGAIVASPQDDGPAAKAGIK-----------AG 369

Query: 391 DIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVLRGDHMEKIPVILEPKPDE 439
           D+IT+VNG+ V +  DL R +     G+K  + V R +  E+I V +   P++
Sbjct: 370 DVITAVNGETVQDPRDLARKVANIAPGEKAALTVWRKNKAEEINVTIAAMPND 407

BLAST of CsaV3_5G027370 vs. ExPASy Swiss-Prot
Match: Q8YG32 (Probable periplasmic serine endoprotease DegP-like OS=Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) OX=224914 GN=htrA PE=3 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 3.7e-52
Identity = 121/293 (41.30%), Postives = 166/293 (56.66%), Query Frame = 0

Query: 151 EVPQGSGSGFVWDKDGHIVTNYHVIRGASDLRVTLADQTTFDAKVVGFDQDKDVAVLSID 210
           E P   GSGFV  +DG++VTN HV+       V L D T  DAK++G D   D+AVL I+
Sbjct: 130 ERPVAQGSGFVISEDGYVVTNNHVVSDGDAYTVVLDDGTELDAKLIGADPRTDLAVLKIN 189

Query: 211 APKDKLRPIPVGISADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDV 270
           APK K   +  G    + VG  V A+GNPFGL  T+T+G++S   R+I +     P  D 
Sbjct: 190 APKRKFVYVAFGDDNKVRVGDWVVAVGNPFGLGGTVTSGIVSARGRDIGAG----PYDDF 249

Query: 271 IQTDAAINPGNSGGPLLDSSGNLIGINTAIYSPSGASSGVGFSIPVDTVSGIVDQLVRFG 330
           IQ DAA+N GNSGGP  D SG +IGINTAI+SPSG S G+ F+IP  T   +VDQL++ G
Sbjct: 250 IQIDAAVNKGNSGGPAFDLSGEVIGINTAIFSPSGGSVGIAFAIPSSTAKQVVDQLIKKG 309

Query: 331 KVTRPILGIKFAP--DQSVEQLGVS---GVLVLDAPANGPAGKAGLLPTKRDAYGRLILG 390
            V R  +G++  P        LG++   G +V     +GPA KAG+             G
Sbjct: 310 SVERGWIGVQIQPVTKDIAASLGLAEEKGAIVASPQDDGPAAKAGIK-----------AG 369

Query: 391 DIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVLRGDHMEKIPVILEPKPDE 439
           D+IT+VNG+ V +  DL R +     G+K  + V R +  E+I V +   P++
Sbjct: 370 DVITAVNGETVQDPRDLARKVANIAPGEKAALTVWRKNKAEEINVTIAAMPND 407

BLAST of CsaV3_5G027370 vs. ExPASy TrEMBL
Match: A0A0A0KP41 (PDZ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G524830 PE=3 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 8.2e-244
Identity = 439/439 (100.00%), Postives = 439/439 (100.00%), Query Frame = 0

Query: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQF 60
           MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQF
Sbjct: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQF 60

Query: 61  SPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120
           SPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA
Sbjct: 61  SPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120

Query: 121 TVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD 180
           TVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD
Sbjct: 121 TVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD 180

Query: 181 LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240
           LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF
Sbjct: 181 LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240

Query: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300
           GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI
Sbjct: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300

Query: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360
           YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA
Sbjct: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360

Query: 361 PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 420
           PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL
Sbjct: 361 PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 420

Query: 421 RGDHMEKIPVILEPKPDES 440
           RGDHMEKIPVILEPKPDES
Sbjct: 421 RGDHMEKIPVILEPKPDES 439

BLAST of CsaV3_5G027370 vs. ExPASy TrEMBL
Match: A0A5A7T7V2 (Protease Do-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G001140 PE=3 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 3.0e-238
Identity = 430/439 (97.95%), Postives = 432/439 (98.41%), Query Frame = 0

Query: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQF 60
           MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNP RHFRYPIFSLLH NKKHSQ 
Sbjct: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPARHFRYPIFSLLHQNKKHSQI 60

Query: 61  SPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120
           SPSI  KLP+PSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA
Sbjct: 61  SPSIHLKLPIPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120

Query: 121 TVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD 180
           TVRLFQENTPSVVYITNLA RQDAFTLDVLEVPQGSGSGFVWDKDGH+VTNYHVIRGASD
Sbjct: 121 TVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHVVTNYHVIRGASD 180

Query: 181 LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240
           LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF
Sbjct: 181 LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240

Query: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300
           GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI
Sbjct: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300

Query: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360
           YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA
Sbjct: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360

Query: 361 PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 420
           PANGPAGKAGL PTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL
Sbjct: 361 PANGPAGKAGLKPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 420

Query: 421 RGDHMEKIPVILEPKPDES 440
           RGDHMEKIPVILEPKPDES
Sbjct: 421 RGDHMEKIPVILEPKPDES 439

BLAST of CsaV3_5G027370 vs. ExPASy TrEMBL
Match: A0A1S3C962 (protease Do-like 1, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498072 PE=3 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 3.0e-238
Identity = 430/439 (97.95%), Postives = 432/439 (98.41%), Query Frame = 0

Query: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHSQF 60
           MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNP RHFRYPIFSLLH NKKHSQ 
Sbjct: 1   MAAAAAFSLLPSSLSRPPKRPPTSFFLSKSISFHNFSNPARHFRYPIFSLLHQNKKHSQI 60

Query: 61  SPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120
           SPSI  KLP+PSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA
Sbjct: 61  SPSIHLKLPIPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDELA 120

Query: 121 TVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD 180
           TVRLFQENTPSVVYITNLA RQDAFTLDVLEVPQGSGSGFVWDKDGH+VTNYHVIRGASD
Sbjct: 121 TVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHVVTNYHVIRGASD 180

Query: 181 LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240
           LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF
Sbjct: 181 LRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240

Query: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300
           GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI
Sbjct: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300

Query: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360
           YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA
Sbjct: 301 YSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360

Query: 361 PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 420
           PANGPAGKAGL PTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL
Sbjct: 361 PANGPAGKAGLKPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVL 420

Query: 421 RGDHMEKIPVILEPKPDES 440
           RGDHMEKIPVILEPKPDES
Sbjct: 421 RGDHMEKIPVILEPKPDES 439

BLAST of CsaV3_5G027370 vs. ExPASy TrEMBL
Match: A0A6J1JTP9 (protease Do-like 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487418 PE=3 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 1.1e-219
Identity = 404/441 (91.61%), Postives = 413/441 (93.65%), Query Frame = 0

Query: 4   AAAFSLLP-----SSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHS 63
           AAAFSL       S LSRPP   PTSFFLSKSI FHNFSNP+ HFRYPIFSLL H KK +
Sbjct: 2   AAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSCHFRYPIFSLLGHTKKRN 61

Query: 64  QFSPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDE 123
           Q S +   KL  PSNPFASICESLL+F+TSV LSF+LFVTDVDPA AFVVTTPRKLQTDE
Sbjct: 62  QISATTDTKLTNPSNPFASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTDE 121

Query: 124 LATVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGA 183
           LATVRLFQENTPSVVYITNLA RQDAFTLDVLEVPQGSGSGFVWDKDG+IVTNYHVIRGA
Sbjct: 122 LATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRGA 181

Query: 184 SDLRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGN 243
           SDLRVTLADQTTFDAKVVGFDQDKDVAVL IDAPKDKLRPIPVGISADLLVGQKVFAIGN
Sbjct: 182 SDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIGN 241

Query: 244 PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT 303
           PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT
Sbjct: 242 PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT 301

Query: 304 AIYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVL 363
           AIYSPSGASSGVGFSIPVDTVSGIVDQLVR+GKVTRPILGIKFAPDQSVEQLGVSGVLVL
Sbjct: 302 AIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLVL 361

Query: 364 DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVE 423
           DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVG+KV VE
Sbjct: 362 DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGEKVIVE 421

Query: 424 VLRGDHMEKIPVILEPKPDES 440
           VLRGDHMEKIPVILEPKPDES
Sbjct: 422 VLRGDHMEKIPVILEPKPDES 442

BLAST of CsaV3_5G027370 vs. ExPASy TrEMBL
Match: A0A6J1E723 (protease Do-like 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111430538 PE=3 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 1.1e-219
Identity = 404/441 (91.61%), Postives = 412/441 (93.42%), Query Frame = 0

Query: 4   AAAFSLLP-----SSLSRPPKRPPTSFFLSKSISFHNFSNPTRHFRYPIFSLLHHNKKHS 63
           AAAFSL       S LSRPP   PTSFFLSKSI FHNFSNP+ HFRYPIFSLL HNKK +
Sbjct: 2   AAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKRN 61

Query: 64  QFSPSILPKLPVPSNPFASICESLLVFSTSVLLSFALFVTDVDPAVAFVVTTPRKLQTDE 123
           Q S +   KL  PSNP ASICESLL+F+TSV LSF+LFVTDVDPA AFVVTTPRKLQTDE
Sbjct: 62  QISATTDTKLTNPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTDE 121

Query: 124 LATVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGA 183
           LATVRLFQENTPSVVYITNLA RQDAFTLDVLEVPQGSGSGFVWDKDG+IVTNYHVIRGA
Sbjct: 122 LATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRGA 181

Query: 184 SDLRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGN 243
           SDLRVTLADQTTFDAKVVGFDQDKDVAVL IDAPKDKLRPIPVGISADLLVGQKVFAIGN
Sbjct: 182 SDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIGN 241

Query: 244 PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT 303
           PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT
Sbjct: 242 PFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINT 301

Query: 304 AIYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVL 363
           AIYSPSGASSGVGFSIPVDTVSGIVDQLVR+GKVTRPILGIKFAPDQSVEQLGVSGVLVL
Sbjct: 302 AIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLVL 361

Query: 364 DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVE 423
           DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKV NGSDLYRILDQCKVGDKV VE
Sbjct: 362 DAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIVE 421

Query: 424 VLRGDHMEKIPVILEPKPDES 440
           VLRGDHMEKIPVILEPKPDES
Sbjct: 422 VLRGDHMEKIPVILEPKPDES 442

BLAST of CsaV3_5G027370 vs. TAIR 10
Match: AT3G27925.1 (DegP protease 1 )

HSP 1 Score: 632.5 bits (1630), Expect = 2.6e-181
Identity = 353/446 (79.15%), Postives = 368/446 (82.51%), Query Frame = 0

Query: 1   MAAAAAFSLLPSS---LSRPPKRPPTSFFLSKSISFHNFSNPTRHFRY-PIFSLLHHNKK 60
           MA   + SLL SS   L  PP    + F LS S S      P R  RY  I S L  N  
Sbjct: 1   MATTTSCSLLLSSTLFLHSPPSSHLSFFNLSSSRSSPISLYPIRSKRYFRILSKLSLNDN 60

Query: 61  HSQFSPSILPKLPVPS-NPFASICESLLVFSTSVLLSFALFVTD--VDPAVAFVVTTPRK 120
           +       L   P  +  PF  +C       TSV LSF+LF     V+ A AFVV+TP+K
Sbjct: 61  NRDDDDDTLHFTPFSAVKPFFLLC-------TSVALSFSLFAASPAVESASAFVVSTPKK 120

Query: 121 LQTDELATVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYH 180
           LQTDELATVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDK GHIVTNYH
Sbjct: 121 LQTDELATVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKQGHIVTNYH 180

Query: 181 VIRGASDLRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKV 240
           VIRGASDLRVTLADQTTFDAKVVGFDQDKDVAVL IDAPK+KLRPIPVG+SADLLVGQKV
Sbjct: 181 VIRGASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKNKLRPIPVGVSADLLVGQKV 240

Query: 241 FAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNL 300
           FAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSG L
Sbjct: 241 FAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGTL 300

Query: 301 IGINTAIYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVS 360
           IGINTAIYSPSGASSGVGFSIPVDTV GIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVS
Sbjct: 301 IGINTAIYSPSGASSGVGFSIPVDTVGGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVS 360

Query: 361 GVLVLDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGD 420
           GVLVLDAP +GPAGKAGL  TKRD YGRL+LGDIITSVNG KV+NGSDLYRILDQCKVGD
Sbjct: 361 GVLVLDAPPSGPAGKAGLQSTKRDGYGRLVLGDIITSVNGTKVSNGSDLYRILDQCKVGD 420

Query: 421 KVTVEVLRGDHMEKIPVILEPKPDES 440
           +VTVEVLRGDH EKI V LEPKPDES
Sbjct: 421 EVTVEVLRGDHKEKISVTLEPKPDES 439

BLAST of CsaV3_5G027370 vs. TAIR 10
Match: AT5G39830.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 304.7 bits (779), Expect = 1.2e-82
Identity = 179/355 (50.42%), Postives = 226/355 (63.66%), Query Frame = 0

Query: 101 DPAVAFV------VTTPRKLQTDELATVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQ 160
           DP+VA V      V     L   E   V+LF++NT SVV I ++ +R       V+E+P+
Sbjct: 93  DPSVATVEDVSPTVFPAGPLFPTEGRIVQLFEKNTYSVVNIFDVTLRPQLKMTGVVEIPE 152

Query: 161 GSGSGFVWDKDGHIVTNYHVIRGASDLRVTLAD-------------QTTFDAKVVGFDQD 220
           G+GSG VWD  G+IVTNYHVI  A     +  D             Q  F+ K+VG D+ 
Sbjct: 153 GNGSGVVWDGQGYIVTNYHVIGNALSRNPSPGDVVGRVNILASDGVQKNFEGKLVGADRA 212

Query: 221 KDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSA 280
           KD+AVL +DAP+  L+PI VG S  L VGQ+  AIGNPFG DHTLT GVISGL R+I S 
Sbjct: 213 KDLAVLKVDAPETLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFS- 272

Query: 281 ATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAIYSPSGASSGVGFSIPVDTVSG 340
            TG  I   IQTDAAINPGNSGGPLLDS GNLIGINTAI++ +G S+GVGF+IP  TV  
Sbjct: 273 QTGVTIGGGIQTDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVLK 332

Query: 341 IVDQLVRFGKVTRPILGIKFAPDQSVEQLGV-SGVLVLDAPANGPAGKAGLLPTKRDAYG 400
           IV QL++F KV R  + I+ APD    QL V +G LVL  P    A KAGL PT R   G
Sbjct: 333 IVPQLIQFSKVLRAGINIELAPDPVANQLNVRNGALVLQVPGKSLAEKAGLHPTSRGFAG 392

Query: 401 RLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVLRGDHMEKIPVILEPK 436
            ++LGDII +V+ K V N ++L +ILD+  VGDKVT+++ RG+   ++ + LE K
Sbjct: 393 NIVLGDIIVAVDDKPVKNKAELMKILDEYSVGDKVTLKIKRGNEDLELKISLEEK 446

BLAST of CsaV3_5G027370 vs. TAIR 10
Match: AT5G39830.2 (Trypsin family protein with PDZ domain )

HSP 1 Score: 279.6 bits (714), Expect = 4.2e-75
Identity = 171/355 (48.17%), Postives = 215/355 (60.56%), Query Frame = 0

Query: 101 DPAVAFV------VTTPRKLQTDELATVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQ 160
           DP+VA V      V     L   E   V+LF++NT SVV I ++ +R       V+E+P+
Sbjct: 93  DPSVATVEDVSPTVFPAGPLFPTEGRIVQLFEKNTYSVVNIFDVTLRPQLKMTGVVEIPE 152

Query: 161 GSGSGFVWDKDGHIVTNYHVIRGASDLRVTLAD-------------QTTFDAKVVGFDQD 220
           G+GSG VWD  G+IVTNYHVI  A     +  D             Q  F+ K+VG D+ 
Sbjct: 153 GNGSGVVWDGQGYIVTNYHVIGNALSRNPSPGDVVGRVNILASDGVQKNFEGKLVGADRA 212

Query: 221 KDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSA 280
           KD+AVL +DAP+  L+PI VG S  L VGQ+  AIGNPFG DHTLT GVISGL R+I S 
Sbjct: 213 KDLAVLKVDAPETLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFS- 272

Query: 281 ATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAIYSPSGASSGVGFSIPVDTVSG 340
            TG  I   IQTDAAINPGNSGGPLLDS GNLIGINTAI++               TV  
Sbjct: 273 QTGVTIGGGIQTDAAINPGNSGGPLLDSKGNLIGINTAIFT--------------QTVLK 332

Query: 341 IVDQLVRFGKVTRPILGIKFAPDQSVEQLGV-SGVLVLDAPANGPAGKAGLLPTKRDAYG 400
           IV QL++F KV R  + I+ APD    QL V +G LVL  P    A KAGL PT R   G
Sbjct: 333 IVPQLIQFSKVLRAGINIELAPDPVANQLNVRNGALVLQVPGKSLAEKAGLHPTSRGFAG 392

Query: 401 RLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTVEVLRGDHMEKIPVILEPK 436
            ++LGDII +V+ K V N ++L +ILD+  VGDKVT+++ RG+   ++ + LE K
Sbjct: 393 NIVLGDIIVAVDDKPVKNKAELMKILDEYSVGDKVTLKIKRGNEDLELKISLEEK 432

BLAST of CsaV3_5G027370 vs. TAIR 10
Match: AT4G18370.1 (DEGP protease 5 )

HSP 1 Score: 194.5 bits (493), Expect = 1.8e-49
Identity = 124/277 (44.77%), Postives = 170/277 (61.37%), Query Frame = 0

Query: 82  LLVFSTSVLLSFALFVTD-----VDPAVAF--VVTTPRKLQTDELATVRLFQENTPSVVY 141
           +++F +S+ L+ +L  ++     ++ A+A         +L+ +E   V LFQ+ +PSVVY
Sbjct: 45  IMIFGSSLALTSSLLGSNQQRLPMESAIALEQFKEKEEELEEEEERNVNLFQKTSPSVVY 104

Query: 142 ITNLAVRQ----DAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIR-------GASDLRV 201
           I  + + +    D  T +     +G+GSGFVWDK GHIVTNYHVI        G    +V
Sbjct: 105 IEAIELPKTSSGDILTDEENGKIEGTGSGFVWDKLGHIVTNYHVIAKLATDQFGLQRCKV 164

Query: 202 TLADQ--TTF--DAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIGNP 261
           +L D   T F  + K+VG D D D+AVL I+    +L P+ +G S DL VGQ  FAIGNP
Sbjct: 165 SLVDAKGTRFSKEGKIVGLDPDNDLAVLKIETEGRELNPVVLGTSNDLRVGQSCFAIGNP 224

Query: 262 FGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTA 321
           +G ++TLT GV+SGL REI S   G+ I + IQTDA IN GNSGGPLLDS G+ IG+NTA
Sbjct: 225 YGYENTLTIGVVSGLGREIPS-PNGKSISEAIQTDADINSGNSGGPLLDSYGHTIGVNTA 284

Query: 322 IYS--PSGASSGVGFSIPVDTVSGIVDQLVRFGKVTR 335
            ++   SG SSGV F+IP+DTV   V  L+ +G   R
Sbjct: 285 TFTRKGSGMSSGVNFAIPIDTVVRTVPYLIVYGTAYR 320

BLAST of CsaV3_5G027370 vs. TAIR 10
Match: AT5G27660.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 134.0 bits (336), Expect = 2.9e-31
Identity = 99/290 (34.14%), Postives = 144/290 (49.66%), Query Frame = 0

Query: 135 ITNLAVRQDAFTLDVLEVPQG---------SGSGFVWDKDGHIVTNYHVIRGASDLR--- 194
           I N A R     ++ L VPQG          GSG + D DG I+T  HV+    ++R   
Sbjct: 119 IANAAARIGPAVVN-LSVPQGFHGISMGKSIGSGTIIDADGTILTCAHVVVDFQNIRHSS 178

Query: 195 -----VTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIG 254
                VTL D  TF+  VV  D   D+A++ I + K  L    +G S+ L  G  V A+G
Sbjct: 179 KGRVDVTLQDGRTFEGVVVNADLQSDIALVKIKS-KTPLPTAKLGFSSKLRPGDWVIAVG 238

Query: 255 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 314
            P  L +T+T G++S + R+ S    G   ++ +QTD +IN GNSGGPL++  G +IG+N
Sbjct: 239 CPLSLQNTVTAGIVSCVDRKSSDLGLGGKHREYLQTDCSINAGNSGGPLVNLDGEVIGVN 298

Query: 315 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAP--DQSVEQLG---- 374
                   A+ G+GFS+P+D+VS I++   + G+V RP +G+K     +  V QL     
Sbjct: 299 IMKVL---AADGLGFSVPIDSVSKIIEHFKKSGRVIRPWIGLKMVELNNLIVAQLKERDP 358

Query: 375 -----VSGVLVLDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKV 397
                  GVLV       PA +AG  P           GD++   +GK V
Sbjct: 359 MFPDVERGVLVPTVIPGSPADRAGFKP-----------GDVVVRFDGKPV 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004142804.11.7e-243100.00protease Do-like 1, chloroplastic [Cucumis sativus] >KGN51390.1 hypothetical pro... [more]
XP_008458761.16.2e-23897.95PREDICTED: protease Do-like 1, chloroplastic isoform X1 [Cucumis melo] >KAA00375... [more]
XP_038890591.11.4e-22694.76protease Do-like 1, chloroplastic isoform X1 [Benincasa hispida][more]
XP_023515810.11.0e-21991.61protease Do-like 1, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022922573.12.2e-21991.61protease Do-like 1, chloroplastic [Cucurbita moschata] >KAG6602439.1 Protease Do... [more]
Match NameE-valueIdentityDescription
O226093.6e-18079.15Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 ... [more]
Q9LU101.7e-8150.42Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 ... [more]
Q2YMX63.7e-5241.30Probable periplasmic serine endoprotease DegP-like OS=Brucella abortus (strain 2... [more]
P0C1143.7e-5241.30Probable periplasmic serine endoprotease DegP-like OS=Brucella abortus biovar 1 ... [more]
Q8YG323.7e-5241.30Probable periplasmic serine endoprotease DegP-like OS=Brucella melitensis biotyp... [more]
Match NameE-valueIdentityDescription
A0A0A0KP418.2e-244100.00PDZ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G524830 PE=3 SV... [more]
A0A5A7T7V23.0e-23897.95Protease Do-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G0... [more]
A0A1S3C9623.0e-23897.95protease Do-like 1, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A6J1JTP91.1e-21991.61protease Do-like 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487418 PE... [more]
A0A6J1E7231.1e-21991.61protease Do-like 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111430538 ... [more]
Match NameE-valueIdentityDescription
AT3G27925.12.6e-18179.15DegP protease 1 [more]
AT5G39830.11.2e-8250.42Trypsin family protein with PDZ domain [more]
AT5G39830.24.2e-7548.17Trypsin family protein with PDZ domain [more]
AT4G18370.11.8e-4944.77DEGP protease 5 [more]
AT5G27660.12.9e-3134.14Trypsin family protein with PDZ domain [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001940Peptidase S1CPRINTSPR00834PROTEASES2Ccoord: 229..253
score: 55.41
coord: 166..178
score: 59.1
coord: 187..207
score: 43.55
coord: 381..393
score: 44.84
coord: 289..306
score: 52.85
coord: 267..284
score: 72.49
IPR001478PDZ domainSMARTSM00228pdz_newcoord: 335..423
e-value: 2.4E-9
score: 47.1
IPR001478PDZ domainPFAMPF13180PDZ_2coord: 335..432
e-value: 1.7E-14
score: 54.0
IPR001478PDZ domainPROSITEPS50106PDZcoord: 326..423
score: 10.9785
IPR036034PDZ superfamilyGENE3D2.30.42.10coord: 335..439
e-value: 6.3E-40
score: 137.2
IPR036034PDZ superfamilySUPERFAMILY50156PDZ domain-likecoord: 293..423
NoneNo IPR availablePFAMPF13365Trypsin_2coord: 157..296
e-value: 7.0E-35
score: 121.2
NoneNo IPR availablePANTHERPTHR43019SERINE ENDOPROTEASE DEGScoord: 57..439
NoneNo IPR availablePANTHERPTHR43019:SF38PROTEASE DO-LIKE 1, CHLOROPLASTICcoord: 57..439
NoneNo IPR availableCDDcd00987PDZ_serine_proteasecoord: 335..430
e-value: 1.96981E-18
score: 78.0651
IPR043504Peptidase S1, PA clan, chymotrypsin-like foldGENE3D2.40.10.10coord: 103..219
e-value: 3.3E-39
score: 135.1
IPR043504Peptidase S1, PA clan, chymotrypsin-like foldGENE3D2.40.10.10coord: 220..334
e-value: 2.5E-41
score: 142.5
IPR009003Peptidase S1, PA clanSUPERFAMILY50494Trypsin-like serine proteasescoord: 122..332

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_5G027370.1CsaV3_5G027370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0005515 protein binding
molecular_function GO:0004252 serine-type endopeptidase activity