CsaV3_5G008460 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_5G008460
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	cucumisin-like
Location	chr5: 5154029 .. 5154854 (+)
RNA-Seq Expression	CsaV3_5G008460
Synteny	CsaV3_5G008460

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAGCAAATTAGAAGATACGAGTTCTACTCCTTTGCATCATAGGGCAATGTTGGAACAAGTTGTTGGGAGGTTTGTATTTTATATATTTATTTATCTATATATTAAGCAAAGAAAAATAAGAAACTATAAAATTTTTGTTATGTTTGTAATTATTTATTGGGACTGTATGATATCCATAAAATGTCAAAAGTTTTTAATACTTTATTGCTTATCTTCTAGCAATTTTGCGCCGAAACACTTGCTGTATAGCTACAAGAGAAGTTTCAATGGATTTGCAGTGAGATTAACTGAAGAAGAAGCTCAAAAGATTGCTTGTATGTATATATTGTTCTAATAATACTTTTTCTTTTTTATATATTTTTAGGTAGTACAAGTTTGTTCAAAGTTGATCACTTTTAATTTATCACATTGCTTTTAATTAATAACAATTTTGGCTATAGGAAAATTTTGGGAAGAAATGTAAATTAATCTGAACTTGTTTAATTAAATTGATTTTAAATATGAGAACAGTGATAATCTCCTTTGAAATTATTTTGTTAACTAAATGTGAAAGATTAATAATATTTTCAGTGAAGGAGGGTGTGGTGTCGGTGTTTCCAAATGGAAAGAAGCATGTTCATACAACAAGGTCTTGGGATTTCATGGGTTTTACACAAAGTGTTCCTCGTGTAAACCAAGTTGAAAGCAACATAGTTGTTGGGGTTCTAGATACCGGAATTTGGCCGGAATCTCCTAGTTTCAATGACACAGATCTCGGACCTCCACCGGCCGGTTGGAAGGGTCAATGCCAAACATCCCCCGACTTTCAATGCAACAGGTGA

mRNA sequence

ATGGGAAGCAAATTAGAAGATACGAGTTCTACTCCTTTGCATCATAGGGCAATGTTGGAACAAGTTGTTGGGAGCAATTTTGCGCCGAAACACTTGCTGTATAGCTACAAGAGAAGTTTCAATGGATTTGCAGTGAGATTAACTGAAGAAGAAGCTCAAAAGATTGCTTGTAGTACAATGAAGGAGGGTGTGGTGTCGGTGTTTCCAAATGGAAAGAAGCATGTTCATACAACAAGGTCTTGGGATTTCATGGGTTTTACACAAAGTGTTCCTCGTGTAAACCAAGTTGAAAGCAACATAGTTGTTGGGGTTCTAGATACCGGAATTTGGCCGGAATCTCCTAGTTTCAATGACACAGATCTCGGACCTCCACCGGCCGGTTGGAAGGGTCAATGCCAAACATCCCCCGACTTTCAATGCAACAGGTGA

Coding sequence (CDS)

ATGGGAAGCAAATTAGAAGATACGAGTTCTACTCCTTTGCATCATAGGGCAATGTTGGAACAAGTTGTTGGGAGCAATTTTGCGCCGAAACACTTGCTGTATAGCTACAAGAGAAGTTTCAATGGATTTGCAGTGAGATTAACTGAAGAAGAAGCTCAAAAGATTGCTTGTAGTACAATGAAGGAGGGTGTGGTGTCGGTGTTTCCAAATGGAAAGAAGCATGTTCATACAACAAGGTCTTGGGATTTCATGGGTTTTACACAAAGTGTTCCTCGTGTAAACCAAGTTGAAAGCAACATAGTTGTTGGGGTTCTAGATACCGGAATTTGGCCGGAATCTCCTAGTTTCAATGACACAGATCTCGGACCTCCACCGGCCGGTTGGAAGGGTCAATGCCAAACATCCCCCGACTTTCAATGCAACAGGTGA

Protein sequence

MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACSTMKEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGPPPAGWKGQCQTSPDFQCNR*

Homology

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BLAST of CsaV3_5G008460 vs. NCBI nr
Match: KAE8648000.1 (hypothetical protein Csa_021430 [Cucumis sativus])

HSP 1 Score: 302.8 bits (774), Expect = 1.6e-78
Identity = 142/142 (100.00%), Postives = 142/142 (100.00%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACSTM 60
           MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACSTM
Sbjct: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACSTM 60

Query: 61  KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTD 120
           KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTD
Sbjct: 61  KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTD 120

Query: 121 LGPPPAGWKGQCQTSPDFQCNR 143
           LGPPPAGWKGQCQTSPDFQCNR
Sbjct: 121 LGPPPAGWKGQCQTSPDFQCNR 142

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BLAST of CsaV3_5G008460 vs. NCBI nr
Match: XP_031741167.1 (cucumisin [Cucumis sativus])

HSP 1 Score: 289.3 bits (739), Expect = 1.9e-74
Identity = 138/142 (97.18%), Postives = 139/142 (97.89%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACSTM 60
           MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIA   +
Sbjct: 25  MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIA---L 84

Query: 61  KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTD 120
           KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTD
Sbjct: 85  KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTD 144

Query: 121 LGPPPAGWKGQCQTSPDFQCNR 143
           LGPPPAGWKGQCQTSPDFQCNR
Sbjct: 145 LGPPPAGWKGQCQTSPDFQCNR 163

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BLAST of CsaV3_5G008460 vs. NCBI nr
Match: XP_008461717.1 (PREDICTED: cucumisin-like [Cucumis melo])

HSP 1 Score: 282.0 bits (720), Expect = 3.0e-72
Identity = 134/142 (94.37%), Postives = 136/142 (95.77%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACSTM 60
           MGS LEDTSSTPLHHRAMLEQV+GSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIA   +
Sbjct: 44  MGSTLEDTSSTPLHHRAMLEQVIGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIA---L 103

Query: 61  KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTD 120
           KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFND D
Sbjct: 104 KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDAD 163

Query: 121 LGPPPAGWKGQCQTSPDFQCNR 143
           L PPPAGWKGQCQTSPDFQCNR
Sbjct: 164 LDPPPAGWKGQCQTSPDFQCNR 182

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BLAST of CsaV3_5G008460 vs. NCBI nr
Match: XP_038891007.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 264.2 bits (674), Expect = 6.4e-67
Identity = 125/142 (88.03%), Postives = 130/142 (91.55%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACSTM 60
           MG KLEDTSSTPLHHR MLEQVVGS FAP+HLLYSYKRSFNGFAV+LT+EEAQKIA   +
Sbjct: 41  MGRKLEDTSSTPLHHRTMLEQVVGSTFAPEHLLYSYKRSFNGFAVKLTKEEAQKIA---L 100

Query: 61  KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTD 120
           KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVP V QVESNIVVGVLDTGIWPESPSFND D
Sbjct: 101 KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPHVKQVESNIVVGVLDTGIWPESPSFNDAD 160

Query: 121 LGPPPAGWKGQCQTSPDFQCNR 143
           L PPPAGWKG+CQ SP FQCNR
Sbjct: 161 LDPPPAGWKGECQISPTFQCNR 179

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BLAST of CsaV3_5G008460 vs. NCBI nr
Match: XP_023548142.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 249.6 bits (636), Expect = 1.6e-62
Identity = 120/142 (84.51%), Postives = 127/142 (89.44%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACSTM 60
           MGSK ED SST L+HR MLEQVVGS+F+PKHLLYSYKRSFNGFAVRLT+EEAQKIA   +
Sbjct: 42  MGSKPEDISSTSLYHRTMLEQVVGSSFSPKHLLYSYKRSFNGFAVRLTKEEAQKIA---L 101

Query: 61  KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTD 120
           +EGVVSVFPN KKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFND  
Sbjct: 102 EEGVVSVFPNEKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDEG 161

Query: 121 LGPPPAGWKGQCQTSPDFQCNR 143
           + PPP  WKG CQ SPDFQCNR
Sbjct: 162 VDPPPKEWKGTCQISPDFQCNR 180

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BLAST of CsaV3_5G008460 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 1.2e-52
Identity = 100/142 (70.42%), Postives = 114/142 (80.28%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACSTM 60
           MG KLED  S  LHHRAMLEQVVGS FAP+ +L++YKRSFNGFAV+LTEEEA+KIA    
Sbjct: 38  MGRKLEDPDSAHLHHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIA---S 97

Query: 61  KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTD 120
            EGVVSVF N    +HTTRSWDF+GF  +VPR +QVESNIVVGVLDTGIWPESPSF+D  
Sbjct: 98  MEGVVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEG 157

Query: 121 LGPPPAGWKGQCQTSPDFQCNR 143
             PPP  WKG C+TS +F+CNR
Sbjct: 158 FSPPPPKWKGTCETSNNFRCNR 176

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BLAST of CsaV3_5G008460 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 3.7e-33
Identity = 71/141 (50.35%), Postives = 95/141 (67.38%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACSTM 60
           MG+  E   S P HH ++L+++VG+  A   L+ SYKRSFNGFA  L++ E+QK+    M
Sbjct: 37  MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKL--QNM 96

Query: 61  KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTD 120
           KE VVSVFP+    + TTRSWDF+GF +   R +  ES+++VGV+D+GIWPES SF+D  
Sbjct: 97  KE-VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEG 156

Query: 121 LGPPPAGWKGQCQTSPDFQCN 142
            GPPP  WKG C+    F CN
Sbjct: 157 FGPPPKKWKGSCKGGLKFACN 174

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BLAST of CsaV3_5G008460 vs. ExPASy Swiss-Prot
Match: Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 5.9e-31
Identity = 68/129 (52.71%), Postives = 83/129 (64.34%), Query Frame = 0


Query: 15  HRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACSTMKEGVVSVFPNGKKH 74
           H ++L+QV G +     L+ SYKRSFNGFA RLTE E   IA     EGVVSVFPN    
Sbjct: 52  HMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIA---EIEGVVSVFPNKILQ 111

Query: 75  VHTTRSWDFMGFTQ--SVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGPPPAGWKGQC 134
           +HTT SWDFMG  +  +  R   +ES+ ++GV+DTGIWPES SF+D   GPPP  WKG C
Sbjct: 112 LHTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVC 171

Query: 135 QTSPDFQCN 142
               +F CN
Sbjct: 172 SGGKNFTCN 177

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BLAST of CsaV3_5G008460 vs. ExPASy Swiss-Prot
Match: O23357 (Subtilisin-like protease SBT4.2 OS=Arabidopsis thaliana OX=3702 GN=SBT4.2 PE=3 SV=3)

HSP 1 Score: 134.4 bits (337), Expect = 1.0e-30
Identity = 68/143 (47.55%), Postives = 92/143 (64.34%), Query Frame = 0


Query: 1   MGSKLEDTSSTPL-HHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACST 60
           MG+     S +P+ HH+ +L++V+ S+    +L+ SY RSFNGFA +LTE E  K+    
Sbjct: 39  MGALPSKISYSPMSHHQNILQEVIESSSVEDYLVRSYGRSFNGFAAKLTESEKDKL---I 98

Query: 61  MKEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDT 120
             EGVVSVFP+    + TTRS++FMG       V +VESN++VGV+D GIWPES SF+D 
Sbjct: 99  GMEGVVSVFPSTVYKLFTTRSYEFMGLGDKSNNVPEVESNVIVGVIDGGIWPESKSFSDE 158

Query: 121 DLGPPPAGWKGQCQTSPDFQCNR 143
            +GP P  WKG C    +F CNR
Sbjct: 159 GIGPIPKKWKGTCAGGTNFTCNR 178

[Close]

BLAST of CsaV3_5G008460 vs. ExPASy Swiss-Prot
Match: F4KGD4 (Subtilisin-like protease SBT4.7 OS=Arabidopsis thaliana OX=3702 GN=SBT4.7 PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.3e-30
Identity = 69/144 (47.92%), Postives = 89/144 (61.81%), Query Frame = 0


Query: 1   MGSKLEDTSSTPL-HHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACST 60
           MGS       TPL HH ++L++V G +     L+ SYKRSFNGFA RLTE E  ++A   
Sbjct: 34  MGSLPSLLEYTPLSHHMSILQEVTGDSSVEGRLVRSYKRSFNGFAARLTESERIRVA--- 93

Query: 61  MKEGVVSVFPNGKKHVHTTRSWDFMGFTQ--SVPRVNQVESNIVVGVLDTGIWPESPSFN 120
             EGVVSVFPN    + TT SWDF+G  +  +  R   +ES+ ++G +D+GIWPES SF+
Sbjct: 94  EMEGVVSVFPNINYKLQTTASWDFLGLKEGKNTKRNLAIESDTIIGFIDSGIWPESESFS 153

Query: 121 DTDLGPPPAGWKGQCQTSPDFQCN 142
           D   GPPP  WKG C    +F CN
Sbjct: 154 DKGFGPPPKKWKGVCSGGKNFTCN 174

[Close]

BLAST of CsaV3_5G008460 vs. ExPASy TrEMBL
Match: A0A0A0KLR1 (Inhibitor I9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G187340 PE=3 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 9.0e-75
Identity = 138/142 (97.18%), Postives = 139/142 (97.89%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACSTM 60
           MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIA   +
Sbjct: 42  MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIA---L 101

Query: 61  KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTD 120
           KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTD
Sbjct: 102 KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTD 161

Query: 121 LGPPPAGWKGQCQTSPDFQCNR 143
           LGPPPAGWKGQCQTSPDFQCNR
Sbjct: 162 LGPPPAGWKGQCQTSPDFQCNR 180

[Close]

BLAST of CsaV3_5G008460 vs. ExPASy TrEMBL
Match: A0A1S3CF95 (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500249 PE=3 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 1.4e-72
Identity = 134/142 (94.37%), Postives = 136/142 (95.77%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACSTM 60
           MGS LEDTSSTPLHHRAMLEQV+GSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIA   +
Sbjct: 44  MGSTLEDTSSTPLHHRAMLEQVIGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIA---L 103

Query: 61  KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTD 120
           KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFND D
Sbjct: 104 KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDAD 163

Query: 121 LGPPPAGWKGQCQTSPDFQCNR 143
           L PPPAGWKGQCQTSPDFQCNR
Sbjct: 164 LDPPPAGWKGQCQTSPDFQCNR 182

[Close]

BLAST of CsaV3_5G008460 vs. ExPASy TrEMBL
Match: A0A6J1CBL9 (LOW QUALITY PROTEIN: cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010158 PE=3 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 1.7e-57
Identity = 111/145 (76.55%), Postives = 124/145 (85.52%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNF---APKHLLYSYKRSFNGFAVRLTEEEAQKIAC 60
           MGSK EDT+STPLHHRAMLE+V+GS F   AP+ LLYSYKRSFNGFAVRLTEEEA K+A 
Sbjct: 42  MGSKPEDTTSTPLHHRAMLEEVIGSTFFLAAPESLLYSYKRSFNGFAVRLTEEEALKVAA 101

Query: 61  STMKEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFN 120
              KEGVVSVFPNGKKH+HTTRSWDFMGF+QSVPR +QVES++VVGVLDTGIWPESPSF 
Sbjct: 102 ---KEGVVSVFPNGKKHLHTTRSWDFMGFSQSVPRAHQVESDVVVGVLDTGIWPESPSFI 161

Query: 121 DTDLGPPPAGWKGQCQTSPDFQCNR 143
           D   GPPPA W G CQ + DF+CN+
Sbjct: 162 DEAFGPPPAKWMGACQAADDFRCNK 183

[Close]

BLAST of CsaV3_5G008460 vs. ExPASy TrEMBL
Match: A0A5A7UBK2 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G001150 PE=3 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 1.1e-56
Identity = 108/142 (76.06%), Postives = 123/142 (86.62%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACSTM 60
           MG+K ED++STP HH  MLE+VVGS+FAP+ LL+SYKRSFNGF V+LTEEEAQKI+    
Sbjct: 39  MGNKPEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISA--- 98

Query: 61  KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTD 120
           KE VVSVFPN KKH+HTTRSWDFMGFTQ+VPRV QVESNIVVGVLD+GIWPESPSF+D  
Sbjct: 99  KENVVSVFPNEKKHLHTTRSWDFMGFTQNVPRVKQVESNIVVGVLDSGIWPESPSFSDVG 158

Query: 121 LGPPPAGWKGQCQTSPDFQCNR 143
            GPPPA WKG CQTS +F+CNR
Sbjct: 159 YGPPPAKWKGACQTSANFRCNR 177

[Close]

BLAST of CsaV3_5G008460 vs. ExPASy TrEMBL
Match: A0A1S3CFE1 (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500264 PE=3 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 1.1e-56
Identity = 108/142 (76.06%), Postives = 123/142 (86.62%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACSTM 60
           MG+K ED++STP HH  MLE+VVGS+FAP+ LL+SYKRSFNGF V+LTEEEAQKI+    
Sbjct: 51  MGNKPEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISA--- 110

Query: 61  KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTD 120
           KE VVSVFPN KKH+HTTRSWDFMGFTQ+VPRV QVESNIVVGVLD+GIWPESPSF+D  
Sbjct: 111 KENVVSVFPNEKKHLHTTRSWDFMGFTQNVPRVKQVESNIVVGVLDSGIWPESPSFSDVG 170

Query: 121 LGPPPAGWKGQCQTSPDFQCNR 143
            GPPPA WKG CQTS +F+CNR
Sbjct: 171 YGPPPAKWKGACQTSANFRCNR 189

[Close]

BLAST of CsaV3_5G008460 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 142.5 bits (358), Expect = 2.6e-34
Identity = 71/141 (50.35%), Postives = 95/141 (67.38%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACSTM 60
           MG+  E   S P HH ++L+++VG+  A   L+ SYKRSFNGFA  L++ E+QK+    M
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKL--QNM 60

Query: 61  KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTD 120
           KE VVSVFP+    + TTRSWDF+GF +   R +  ES+++VGV+D+GIWPES SF+D  
Sbjct: 61  KE-VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEG 120

Query: 121 LGPPPAGWKGQCQTSPDFQCN 142
            GPPP  WKG C+    F CN
Sbjct: 121 FGPPPKKWKGSCKGGLKFACN 138

[Close]

BLAST of CsaV3_5G008460 vs. TAIR 10
Match: AT5G59090.3 (subtilase 4.12 )

HSP 1 Score: 136.3 bits (342), Expect = 1.9e-32
Identity = 68/129 (52.71%), Postives = 83/129 (64.34%), Query Frame = 0


Query: 15  HRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACSTMKEGVVSVFPNGKKH 74
           H ++L+QV G +     L+ SYKRSFNGFA RLTE E   IA     EGVVSVFPN    
Sbjct: 52  HMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIA-----EGVVSVFPNKILQ 111

Query: 75  VHTTRSWDFMGFTQ--SVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGPPPAGWKGQC 134
           +HTT SWDFMG  +  +  R   +ES+ ++GV+DTGIWPES SF+D   GPPP  WKG C
Sbjct: 112 LHTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVC 171

Query: 135 QTSPDFQCN 142
               +F CN
Sbjct: 172 SGGKNFTCN 175

[Close]

BLAST of CsaV3_5G008460 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 135.2 bits (339), Expect = 4.2e-32
Identity = 68/129 (52.71%), Postives = 83/129 (64.34%), Query Frame = 0


Query: 15  HRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACSTMKEGVVSVFPNGKKH 74
           H ++L+QV G +     L+ SYKRSFNGFA RLTE E   IA     EGVVSVFPN    
Sbjct: 52  HMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIA---EIEGVVSVFPNKILQ 111

Query: 75  VHTTRSWDFMGFTQ--SVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGPPPAGWKGQC 134
           +HTT SWDFMG  +  +  R   +ES+ ++GV+DTGIWPES SF+D   GPPP  WKG C
Sbjct: 112 LHTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVC 171

Query: 135 QTSPDFQCN 142
               +F CN
Sbjct: 172 SGGKNFTCN 177

[Close]

BLAST of CsaV3_5G008460 vs. TAIR 10
Match: AT5G59090.2 (subtilase 4.12 )

HSP 1 Score: 135.2 bits (339), Expect = 4.2e-32
Identity = 68/129 (52.71%), Postives = 83/129 (64.34%), Query Frame = 0


Query: 15  HRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACSTMKEGVVSVFPNGKKH 74
           H ++L+QV G +     L+ SYKRSFNGFA RLTE E   IA     EGVVSVFPN    
Sbjct: 52  HMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIA---EIEGVVSVFPNKILQ 111

Query: 75  VHTTRSWDFMGFTQ--SVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGPPPAGWKGQC 134
           +HTT SWDFMG  +  +  R   +ES+ ++GV+DTGIWPES SF+D   GPPP  WKG C
Sbjct: 112 LHTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVC 171

Query: 135 QTSPDFQCN 142
               +F CN
Sbjct: 172 SGGKNFTCN 177

[Close]

BLAST of CsaV3_5G008460 vs. TAIR 10
Match: AT4G15040.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 134.4 bits (337), Expect = 7.1e-32
Identity = 68/143 (47.55%), Postives = 92/143 (64.34%), Query Frame = 0


Query: 1   MGSKLEDTSSTPL-HHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIACST 60
           MG+     S +P+ HH+ +L++V+ S+    +L+ SY RSFNGFA +LTE E  K+    
Sbjct: 1   MGALPSKISYSPMSHHQNILQEVIESSSVEDYLVRSYGRSFNGFAAKLTESEKDKL---I 60

Query: 61  MKEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDT 120
             EGVVSVFP+    + TTRS++FMG       V +VESN++VGV+D GIWPES SF+D 
Sbjct: 61  GMEGVVSVFPSTVYKLFTTRSYEFMGLGDKSNNVPEVESNVIVGVIDGGIWPESKSFSDE 120

Query: 121 DLGPPPAGWKGQCQTSPDFQCNR 143
            +GP P  WKG C    +F CNR
Sbjct: 121 GIGPIPKKWKGTCAGGTNFTCNR 140

The following BLAST results are available for this feature:

BLAST of CsaV3_5G008460 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8648000.1	1.6e-78	100.00	hypothetical protein Csa_021430 [Cucumis sativus]	[more]
XP_031741167.1	1.9e-74	97.18	cucumisin [Cucumis sativus]	[more]
XP_008461717.1	3.0e-72	94.37	PREDICTED: cucumisin-like [Cucumis melo]	[more]
XP_038891007.1	6.4e-67	88.03	cucumisin-like [Benincasa hispida]	[more]
XP_023548142.1	1.6e-62	84.51	cucumisin-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CsaV3_5G008460 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39547	1.2e-52	70.42	Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
Q9FIF8	3.7e-33	50.35	Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S...	[more]
Q8L7D2	5.9e-31	52.71	Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2...	[more]
O23357	1.0e-30	47.55	Subtilisin-like protease SBT4.2 OS=Arabidopsis thaliana OX=3702 GN=SBT4.2 PE=3 S...	[more]
F4KGD4	1.3e-30	47.92	Subtilisin-like protease SBT4.7 OS=Arabidopsis thaliana OX=3702 GN=SBT4.7 PE=3 S...	[more]

BLAST of CsaV3_5G008460 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KLR1	9.0e-75	97.18	Inhibitor I9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G18734...	[more]
A0A1S3CF95	1.4e-72	94.37	cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500249 PE=3 SV=1	[more]
A0A6J1CBL9	1.7e-57	76.55	LOW QUALITY PROTEIN: cucumisin-like OS=Momordica charantia OX=3673 GN=LOC1110101...	[more]
A0A5A7UBK2	1.1e-56	76.06	Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G00115...	[more]
A0A1S3CFE1	1.1e-56	76.06	cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500264 PE=3 SV=1	[more]

BLAST of CsaV3_5G008460 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59190.1	2.6e-34	50.35	subtilase family protein	[more]
AT5G59090.3	1.9e-32	52.71	subtilase 4.12	[more]
AT5G59090.1	4.2e-32	52.71	subtilase 4.12	[more]
AT5G59090.2	4.2e-32	52.71	subtilase 4.12	[more]
AT4G15040.1	7.1e-32	47.55	Subtilisin-like serine endopeptidase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 8..72 e-value: 1.9E-10 score: 41.3
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 1..76 e-value: 4.8E-19 score: 70.6
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 77..142 e-value: 4.5E-17 score: 64.1
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 36..136
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 114..136
None	No IPR available	PANTHER	PTHR10795:SF713	SUBTILISIN-LIKE SERINE ENDOPEPTIDASE FAMILY PROTEIN	coord: 11..141
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 11..141

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_5G008460.1	CsaV3_5G008460.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity