CsaV3_5G004930 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_5G004930
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	4a-hydroxytetrahydrobiopterin dehydratase
Location	chr5: 3185607 .. 3187634 (-)
RNA-Seq Expression	CsaV3_5G004930
Synteny	CsaV3_5G004930

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTAATTTATTAAAAAAATTACAATAAGTTCAATCTATGATAATCATGTACGTATAATTTAATTTTTTACAAAAATTTTATACTCATATATTGTAAGTTTGGTAGATTTTATGCAAAATTAGTTGATATGCATATGAACTCTCGTATAAGATTGAAGATGGTATATTATAAAAACAAAAAAAAACTTACAAAATTTGACCAAGCATAAGCTATTGGTTGAAGATAAGAAGATATTCTATCGATGTCAGACTTGAGAAAATTCAAACCAATAATATTTCAGAAAAAAAATTCAAATGTATTCCTTCGTAATCGAGATTGAATTGAACGTTCACTGGACCGACCATAAGCAATTTTCATGGCGGTTTTGTTGAAATCCGAGTTACAATATTAAAAAAGAATAATGATTTAACAAAATTTGGTTTGTAGCTTGTGTTTGCTTTCATTGCGAAAGGCTAAATATCAAAATAGAAAGCTATCCACTAAATTTTCAATAGAAAAGAAAGAAAGGTAAACGGCCAAGTGAGCTTCACTCTCATCCTTTAACCATGGCCACAACCTCATCCACCCATCTCCACCTCCACCTCCCTCTCCTCCCTCTTCCCCAACCCCACCACCGCACCTTCCTTCCCTTACCCACCACCCTCCTCCGCCGTAACCTCCGTTCCCTCACCGTTTCCGTGGGAACAGACATACTAGGAGACTTCGGCGCCAGAGACCCATTTCCGGAGGAAATCGCGAGCAATTTCGGGGAAAAAGTTATCGGATTTTCCGATACAGAGCACAAGATTCTGATCCCTAATGCCCGAGCCTTGGCTCTCTCTGAACAAGAGTGTGTTCCAGTTTCTTCATTGCAGGCTCCAATGGCAGAGGATGAAGCCAAAAAGCTCCTGAGGAAGGTATATTGTGTGTTTGATTTTGAAATTGGTTTTTTGATTTGATTTGATATGAAGTTTTTTGTTGTTAGGTTGTGGGTTGGAAATTGGTGGATGGTGGAAGAGGGGAATTAAAGCTTCAATGTTTATGGAAATTGAGGGATTTTAAATGCAGTGTTGAGCTGATTAATAGAATTTGTAAAGTTGTTGAGAATGTTGGCCATTTTCCTGATCTTCATTTGGAGCAACCCAATCAAGTTAGAGCTGAGCTTTGGACTTCATCCATTGGTATTGAATACTGATATCTCTTCTTCTCCATTACTCCTCATTTTGTGTTTGTTAAAATGTCTTAGGTGAGTGATTTTGGAATTGAATACATGCAGGAGGGTTGAGCATGAATGATTATATAGTAGCTGCAAAAATTGATGAGATTAAAACATCAGATCTTGCTCCCAGGAAAAGAGCTTGGGCATGAACTCAACCACTTCTTTTTTCTTGATTTTCTTTTCCATATTTTAATTTGTATGAATAAAACCTTTTCTTTTTCATCTTCTTAATTCTCTTAAAGCCTCTTGTATACGAACTGTTTCATAATTTGATTTTTTTTGGTCTGATGATAAGCAAGGCATTTCAAATTTAGTGTTATGCTGCTCTACACTCATGCGAAGTTTGAATGAAATTATCAAAATAGGTATGCCTATGTGAAAAAGTTCATATGGCTTTTTGACGACGATCCACTTTGCAAGCACCATCGAACAAAACGTCGTCGCTCACGCTTTGTTCAATTATTCGTATGGAGTTATTGTATCCACGAAGTAGCACATCCAAAGATTTGTTCATGGAGAGCTTTATAATTTAGTAAAAATAATCTACAAAAAATGTAAAGAGTAGGATTAGATGATCGTTTTACAGTAGGTCAACAACTATATTGAAATTATTTCAATTTTTTGTATATACAAAGAGAGCTATAAAGTTTATTGTATACATTGCTCCATTAATTCTCTCGCCAGTTATACATTGTCTAGCAGTGAGTGAGATCTTTTTGACTCTCTGTCTCATTGCTGGCTTTCTTCTTTAATTCGTAAAAAGATATCGAATTGTAACCATATTTACAAGCGCACAAATTTTATTAATAAAAGTTGTCATTAGCTTTCCAA

mRNA sequence

ATGGCCACAACCTCATCCACCCATCTCCACCTCCACCTCCCTCTCCTCCCTCTTCCCCAACCCCACCACCGCACCTTCCTTCCCTTACCCACCACCCTCCTCCGCCGTAACCTCCGTTCCCTCACCGTTTCCGTGGGAACAGACATACTAGGAGACTTCGGCGCCAGAGACCCATTTCCGGAGGAAATCGCGAGCAATTTCGGGGAAAAAGTTATCGGATTTTCCGATACAGAGCACAAGATTCTGATCCCTAATGCCCGAGCCTTGGCTCTCTCTGAACAAGAGTGTGTTCCAGTTTCTTCATTGCAGGCTCCAATGGCAGAGGATGAAGCCAAAAAGCTCCTGAGGAAGGTTGTGGGTTGGAAATTGGTGGATGGTGGAAGAGGGGAATTAAAGCTTCAATGTTTATGGAAATTGAGGGATTTTAAATGCAGTGTTGAGCTGATTAATAGAATTTGTAAAGTTGTTGAGAATGTTGGCCATTTTCCTGATCTTCATTTGGAGCAACCCAATCAAGTTAGAGCTGAGCTTTGGACTTCATCCATTGGAGGGTTGAGCATGAATGATTATATAGTAGCTGCAAAAATTGATGAGATTAAAACATCAGATCTTGCTCCCAGGAAAAGAGCTTGGGCATGA

Coding sequence (CDS)

ATGGCCACAACCTCATCCACCCATCTCCACCTCCACCTCCCTCTCCTCCCTCTTCCCCAACCCCACCACCGCACCTTCCTTCCCTTACCCACCACCCTCCTCCGCCGTAACCTCCGTTCCCTCACCGTTTCCGTGGGAACAGACATACTAGGAGACTTCGGCGCCAGAGACCCATTTCCGGAGGAAATCGCGAGCAATTTCGGGGAAAAAGTTATCGGATTTTCCGATACAGAGCACAAGATTCTGATCCCTAATGCCCGAGCCTTGGCTCTCTCTGAACAAGAGTGTGTTCCAGTTTCTTCATTGCAGGCTCCAATGGCAGAGGATGAAGCCAAAAAGCTCCTGAGGAAGGTTGTGGGTTGGAAATTGGTGGATGGTGGAAGAGGGGAATTAAAGCTTCAATGTTTATGGAAATTGAGGGATTTTAAATGCAGTGTTGAGCTGATTAATAGAATTTGTAAAGTTGTTGAGAATGTTGGCCATTTTCCTGATCTTCATTTGGAGCAACCCAATCAAGTTAGAGCTGAGCTTTGGACTTCATCCATTGGAGGGTTGAGCATGAATGATTATATAGTAGCTGCAAAAATTGATGAGATTAAAACATCAGATCTTGCTCCCAGGAAAAGAGCTTGGGCATGA

Protein sequence

MATTSSTHLHLHLPLLPLPQPHHRTFLPLPTTLLRRNLRSLTVSVGTDILGDFGARDPFPEEIASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKKLLRKVVGWKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQVRAELWTSSIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA*

Homology

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BLAST of CsaV3_5G004930 vs. NCBI nr
Match: XP_004143676.1 (probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic [Cucumis sativus] >KGN50323.1 hypothetical protein Csa_000223 [Cucumis sativus])

HSP 1 Score: 431.8 bits (1109), Expect = 3.4e-117
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MATTSSTHLHLHLPLLPLPQPHHRTFLPLPTTLLRRNLRSLTVSVGTDILGDFGARDPFP 60
           MATTSSTHLHLHLPLLPLPQPHHRTFLPLPTTLLRRNLRSLTVSVGTDILGDFGARDPFP
Sbjct: 1   MATTSSTHLHLHLPLLPLPQPHHRTFLPLPTTLLRRNLRSLTVSVGTDILGDFGARDPFP 60

Query: 61  EEIASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKKLLRKVVG 120
           EEIASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKKLLRKVVG
Sbjct: 61  EEIASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKKLLRKVVG 120

Query: 121 WKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQVRAELWTS 180
           WKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQVRAELWTS
Sbjct: 121 WKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQVRAELWTS 180

Query: 181 SIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 213
           SIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA
Sbjct: 181 SIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 212

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BLAST of CsaV3_5G004930 vs. NCBI nr
Match: XP_008436971.1 (PREDICTED: putative pterin-4-alpha-carbinolamine dehydratase [Cucumis melo] >KAA0055083.1 putative pterin-4-alpha-carbinolamine dehydratase [Cucumis melo var. makuwa])

HSP 1 Score: 390.6 bits (1002), Expect = 8.8e-105
Identity = 192/212 (90.57%), Postives = 200/212 (94.34%), Query Frame = 0


Query: 1   MATTSSTHLHLHLPLLPLPQPHHRTFLPLPTTLLRRNLRSLTVSVGTDILGDFGARDPFP 60
           MATTSSTHLHLHLPLLPLPQPH   F  LPTTLLRR+ RS+TVSVGTDILGDFGARDPFP
Sbjct: 1   MATTSSTHLHLHLPLLPLPQPHRPRFSSLPTTLLRRSRRSVTVSVGTDILGDFGARDPFP 60

Query: 61  EEIASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKKLLRKVVG 120
            EI SNFG+KVIG+S+TEHKILIPNARALALS+QECVPVSSLQAPMAEDEAK LL+KVVG
Sbjct: 61  AEIESNFGDKVIGYSNTEHKILIPNARALALSQQECVPVSSLQAPMAEDEAKMLLKKVVG 120

Query: 121 WKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQVRAELWTS 180
           WKLVD G G LKLQCLWKLRDFKCSVELINRIC+VVENVGHFPDLHLEQPNQVRAELWTS
Sbjct: 121 WKLVDDGSGGLKLQCLWKLRDFKCSVELINRICQVVENVGHFPDLHLEQPNQVRAELWTS 180

Query: 181 SIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 213
           SIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA
Sbjct: 181 SIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 212

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BLAST of CsaV3_5G004930 vs. NCBI nr
Match: XP_038877111.1 (probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic [Benincasa hispida])

HSP 1 Score: 352.4 bits (903), Expect = 2.7e-93
Identity = 180/214 (84.11%), Postives = 187/214 (87.38%), Query Frame = 0


Query: 1   MATTSSTHLHLHLPLLPLPQPHHRTFLPLPTTLLRRNLR--SLTVSVGTDILGDFGARDP 60
           MAT SST  HLHLPLLPLP  H R F   P  LLRRN R  S+ VSVG D LGDFGARDP
Sbjct: 1   MATASST--HLHLPLLPLPH-HRRRFSSPPAILLRRNRRSASVIVSVGADKLGDFGARDP 60

Query: 61  FPEEIASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKKLLRKV 120
           FP EI SNFGEKV+GFSDTEHKILIPN RALALS+QECVPVSSLQAPMAEDEA+KLL+KV
Sbjct: 61  FPAEIESNFGEKVVGFSDTEHKILIPNVRALALSQQECVPVSSLQAPMAEDEAQKLLKKV 120

Query: 121 VGWKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQVRAELW 180
           VGW+LVD GRGE KLQCLWKLRDFKC VELINRI KVVENVGHFPDLHLEQPNQVRAELW
Sbjct: 121 VGWRLVDDGRGEFKLQCLWKLRDFKCCVELINRISKVVENVGHFPDLHLEQPNQVRAELW 180

Query: 181 TSSIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 213
           TS IGGLSMND+IVAAKIDEIKTSDLAPRKR WA
Sbjct: 181 TSPIGGLSMNDFIVAAKIDEIKTSDLAPRKRVWA 211

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BLAST of CsaV3_5G004930 vs. NCBI nr
Match: XP_022996033.1 (uncharacterized protein LOC111491359 [Cucurbita maxima] >XP_023532646.1 uncharacterized protein LOC111794746 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 307.4 bits (786), Expect = 9.8e-80
Identity = 154/214 (71.96%), Postives = 174/214 (81.31%), Query Frame = 0


Query: 1   MATTSSTHLHLHLPLLPLPQPHHRTFLP--LPTTLLRRNLRSLTVSVGTDILGDFGARDP 60
           MATT +     + PLL LP+PH R  LP    TTLLRR LR  T+       GDFGARDP
Sbjct: 1   MATTPTA----NFPLLRLPRPHSRFSLPPSPSTTLLRRKLRPATIRAAVVGDGDFGARDP 60

Query: 61  FPEEIASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKKLLRKV 120
           FP EI SNFG+KVIGFS+TEHKILIPN RALALS+Q+CVP+SSLQAPM EDEA+KLL+KV
Sbjct: 61  FPAEIESNFGDKVIGFSNTEHKILIPNVRALALSQQQCVPISSLQAPMPEDEAQKLLKKV 120

Query: 121 VGWKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQVRAELW 180
           VGW+LVD GRG+ KL+CLWKLRDFKC VELINRI   V++  HFPD+HL+QPNQV AELW
Sbjct: 121 VGWRLVDNGRGQFKLRCLWKLRDFKCCVELINRISNAVDDANHFPDIHLDQPNQVTAELW 180

Query: 181 TSSIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 213
           TS+IGGLSMND+IVAAKIDEIKT DLA RKRAWA
Sbjct: 181 TSAIGGLSMNDFIVAAKIDEIKTIDLAARKRAWA 210

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BLAST of CsaV3_5G004930 vs. NCBI nr
Match: XP_022957679.1 (uncharacterized protein LOC111459142 [Cucurbita moschata] >KAG7035558.1 hypothetical protein SDJN02_02355 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 305.1 bits (780), Expect = 4.9e-79
Identity = 149/209 (71.29%), Postives = 172/209 (82.30%), Query Frame = 0


Query: 6   STHLHLHLPLLPLPQPHHRTFLPL--PTTLLRRNLRSLTVSVGTDILGDFGARDPFPEEI 65
           +T  + + PLL LP+PH R  LP   P+T LRR LR  T+       GDFGARDPFP EI
Sbjct: 2   ATAPNANFPLLRLPRPHSRFSLPQTPPSTFLRRKLRPATIRAAVVGDGDFGARDPFPAEI 61

Query: 66  ASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKKLLRKVVGWKL 125
            SNFG+KVIGFS+TEHKILIPN RALALS+Q+CVP+SSLQAPM EDEA+KLL+KVVGW+L
Sbjct: 62  ESNFGDKVIGFSNTEHKILIPNVRALALSQQQCVPISSLQAPMPEDEAQKLLKKVVGWRL 121

Query: 126 VDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQVRAELWTSSIG 185
           VD GRG+ KL+CLWKLRDFKC VELINRI   V++  HFPD+HL+QPNQV AELWTS+IG
Sbjct: 122 VDNGRGQFKLRCLWKLRDFKCCVELINRISNAVDDANHFPDIHLDQPNQVTAELWTSAIG 181

Query: 186 GLSMNDYIVAAKIDEIKTSDLAPRKRAWA 213
           GLSMND+I+AAKIDEIKT DLA RKRAWA
Sbjct: 182 GLSMNDFIIAAKIDEIKTIDLAARKRAWA 210

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BLAST of CsaV3_5G004930 vs. ExPASy Swiss-Prot
Match: Q9LU63 (Probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATP1 PE=1 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 1.1e-65
Identity = 131/219 (59.82%), Postives = 161/219 (73.52%), Query Frame = 0


Query: 2   ATTSSTHLHLHLPLLPLPQPHHRTFLPL-----PTTLLRRNLRSLTV---SVGTDILGDF 61
           AT+SS   ++    L L QP  R+ L +     P +   R L  LTV   ++  D LGDF
Sbjct: 3   ATSSSPPCNISASSLLLRQP-SRSILKVFGLLPPVSRNNRKLGRLTVTRSNLAQDFLGDF 62

Query: 62  GARDPFPEEIASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKK 121
           GARDP+PEEIAS FG+KV+G   TEHKILIPNA  L+LS+ +C PVSS Q P++ D+A+ 
Sbjct: 63  GARDPYPEEIASQFGDKVLGCQSTEHKILIPNASVLSLSQLQCSPVSSSQPPLSGDDART 122

Query: 122 LLRKVVGWKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQV 181
           LL KV+GW +VD   G LK++C+WK+RDF C VELINRI KV E  GH+P LHLE P QV
Sbjct: 123 LLHKVLGWSIVDNEAGGLKIRCMWKVRDFGCGVELINRIHKVAEASGHYPSLHLESPTQV 182

Query: 182 RAELWTSSIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 213
           RAEL+TSSIGGLSMND+I+AAKID+IKTSDL+PRKRAWA
Sbjct: 183 RAELFTSSIGGLSMNDFIMAAKIDDIKTSDLSPRKRAWA 220

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BLAST of CsaV3_5G004930 vs. ExPASy Swiss-Prot
Match: Q6QJ72 (Pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PDL2 PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.1e-20
Identity = 49/120 (40.83%), Postives = 75/120 (62.50%), Query Frame = 0


Query: 91  LSEQECVPVSSLQ-APMAEDEAKKLLRKVVGWKLVDGGRGELKLQCLWKLRDFKCSVELI 150
           LS ++CVP ++     M E  A+ LL+KV GW L +     LKL   W+++ F   ++  
Sbjct: 66  LSTKKCVPCNAKDLRAMTEQSAQDLLQKVAGWDLAN-DNDTLKLHRSWRVKSFTKGLDFF 125

Query: 151 NRICKVVENVGHFPDLHLEQPNQVRAELWTSSIGGLSMNDYIVAAKIDEIKTSDLAPRKR 210
            R+  + E+ GH PDLHL   N V+ E+WT +IGGL+ ND+I+AAKI+E++  DL  +K+
Sbjct: 126 QRVADIAESEGHHPDLHLVGWNNVKIEIWTHAIGGLTENDFILAAKINELQVEDLLRKKK 184

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BLAST of CsaV3_5G004930 vs. ExPASy Swiss-Prot
Match: B0JQV0 (Putative pterin-4-alpha-carbinolamine dehydratase OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=MAE_39970 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.2e-11
Identity = 37/109 (33.94%), Postives = 61/109 (55.96%), Query Frame = 0


Query: 91  LSEQECVPVSSLQAPMAEDEAKKLLRKVVGWKLVDGGRGELKLQCLWKLRDFKCSVELIN 150
           L++Q+C P  S  +P+  +E   L  K+  W L++   G  +LQ L+K  +F+ ++   N
Sbjct: 4   LAQQKCQPYQSGSSPITAEEITALQAKIPDWNLLE-YEGIPRLQKLYKFANFQGAIAFTN 63

Query: 151 RICKVVENVGHFPDLHLEQPNQVRAELWTSSIGGLSMNDYIVAAKIDEI 200
            + +  E  GH P L L +  +V    WT  +GGL  ND+I+AA+ D+I
Sbjct: 64  AVGEAAEKEGHHPAL-LTEWGKVTVSWWTHDVGGLHQNDFIMAARTDDI 110

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BLAST of CsaV3_5G004930 vs. ExPASy Swiss-Prot
Match: Q60BH8 (Putative pterin-4-alpha-carbinolamine dehydratase OS=Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) OX=243233 GN=MCA0497 PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.1e-09
Identity = 36/112 (32.14%), Postives = 61/112 (54.46%), Query Frame = 0


Query: 88  ALALSEQECVPVSSLQAPMAEDEAKKLLRKVVGWKLVDGGRGELKLQCLWKLRDFKCSVE 147
           A +L+ ++C P      P+  +EA+KLL  V  W+L D      KL+  ++  +F  +++
Sbjct: 3   ACSLTAKQCTPCQGGIPPLTAEEAEKLLVHVPRWELKDAA---TKLKRTFRFENFMEALD 62

Query: 148 LINRICKVVENVGHFPDLHLEQPNQVRAELWTSSIGGLSMNDYIVAAKIDEI 200
              ++ ++ E  GH PD+ +      R E  T  I GL  ND+I+AAK+DE+
Sbjct: 63  FARKVGELCEAEGHHPDIGIGW-GYCRVEFQTHKINGLHENDFIMAAKVDEL 110

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BLAST of CsaV3_5G004930 vs. ExPASy Swiss-Prot
Match: B9LPE3 (Putative pterin-4-alpha-carbinolamine dehydratase OS=Halorubrum lacusprofundi (strain ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34) OX=416348 GN=Hlac_1646 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.5e-09
Identity = 35/114 (30.70%), Postives = 62/114 (54.39%), Query Frame = 0


Query: 91  LSEQECVPVSSLQAPMAEDEAKKLLRKV-VGWKLVDGGRGELKLQCLWKLRDFKCSVELI 150
           L+++   P      P+ +DE  + L ++   W++VD    +  L+  ++  DF  ++   
Sbjct: 5   LADEPVEPADEGAEPLTDDEYAEYLAELGPVWEVVD----DHHLEATYEFDDFADALAFT 64

Query: 151 NRICKVVENVGHFPDLHLEQPNQVRAELWTSSIGGLSMNDYIVAAKIDEIKTSD 204
           N I ++ E  GH PDLHL    +V  E+W+  IGGL  +D+++AA++D I   D
Sbjct: 65  NEIGELAEAEGHHPDLHLSW-GEVGVEMWSHDIGGLHESDFVMAARMDRIYDGD 113

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BLAST of CsaV3_5G004930 vs. ExPASy TrEMBL
Match: A0A0A0KL10 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis sativus OX=3659 GN=Csa_5G167150 PE=3 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 1.7e-117
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MATTSSTHLHLHLPLLPLPQPHHRTFLPLPTTLLRRNLRSLTVSVGTDILGDFGARDPFP 60
           MATTSSTHLHLHLPLLPLPQPHHRTFLPLPTTLLRRNLRSLTVSVGTDILGDFGARDPFP
Sbjct: 1   MATTSSTHLHLHLPLLPLPQPHHRTFLPLPTTLLRRNLRSLTVSVGTDILGDFGARDPFP 60

Query: 61  EEIASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKKLLRKVVG 120
           EEIASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKKLLRKVVG
Sbjct: 61  EEIASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKKLLRKVVG 120

Query: 121 WKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQVRAELWTS 180
           WKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQVRAELWTS
Sbjct: 121 WKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQVRAELWTS 180

Query: 181 SIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 213
           SIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA
Sbjct: 181 SIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 212

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BLAST of CsaV3_5G004930 vs. ExPASy TrEMBL
Match: A0A5A7UGL0 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00090 PE=3 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 4.3e-105
Identity = 192/212 (90.57%), Postives = 200/212 (94.34%), Query Frame = 0


Query: 1   MATTSSTHLHLHLPLLPLPQPHHRTFLPLPTTLLRRNLRSLTVSVGTDILGDFGARDPFP 60
           MATTSSTHLHLHLPLLPLPQPH   F  LPTTLLRR+ RS+TVSVGTDILGDFGARDPFP
Sbjct: 1   MATTSSTHLHLHLPLLPLPQPHRPRFSSLPTTLLRRSRRSVTVSVGTDILGDFGARDPFP 60

Query: 61  EEIASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKKLLRKVVG 120
            EI SNFG+KVIG+S+TEHKILIPNARALALS+QECVPVSSLQAPMAEDEAK LL+KVVG
Sbjct: 61  AEIESNFGDKVIGYSNTEHKILIPNARALALSQQECVPVSSLQAPMAEDEAKMLLKKVVG 120

Query: 121 WKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQVRAELWTS 180
           WKLVD G G LKLQCLWKLRDFKCSVELINRIC+VVENVGHFPDLHLEQPNQVRAELWTS
Sbjct: 121 WKLVDDGSGGLKLQCLWKLRDFKCSVELINRICQVVENVGHFPDLHLEQPNQVRAELWTS 180

Query: 181 SIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 213
           SIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA
Sbjct: 181 SIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 212

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BLAST of CsaV3_5G004930 vs. ExPASy TrEMBL
Match: A0A1S3ASJ9 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis melo OX=3656 GN=LOC103482540 PE=3 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 4.3e-105
Identity = 192/212 (90.57%), Postives = 200/212 (94.34%), Query Frame = 0


Query: 1   MATTSSTHLHLHLPLLPLPQPHHRTFLPLPTTLLRRNLRSLTVSVGTDILGDFGARDPFP 60
           MATTSSTHLHLHLPLLPLPQPH   F  LPTTLLRR+ RS+TVSVGTDILGDFGARDPFP
Sbjct: 1   MATTSSTHLHLHLPLLPLPQPHRPRFSSLPTTLLRRSRRSVTVSVGTDILGDFGARDPFP 60

Query: 61  EEIASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKKLLRKVVG 120
            EI SNFG+KVIG+S+TEHKILIPNARALALS+QECVPVSSLQAPMAEDEAK LL+KVVG
Sbjct: 61  AEIESNFGDKVIGYSNTEHKILIPNARALALSQQECVPVSSLQAPMAEDEAKMLLKKVVG 120

Query: 121 WKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQVRAELWTS 180
           WKLVD G G LKLQCLWKLRDFKCSVELINRIC+VVENVGHFPDLHLEQPNQVRAELWTS
Sbjct: 121 WKLVDDGSGGLKLQCLWKLRDFKCSVELINRICQVVENVGHFPDLHLEQPNQVRAELWTS 180

Query: 181 SIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 213
           SIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA
Sbjct: 181 SIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 212

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BLAST of CsaV3_5G004930 vs. ExPASy TrEMBL
Match: A0A6J1K9P8 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111491359 PE=3 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 4.7e-80
Identity = 154/214 (71.96%), Postives = 174/214 (81.31%), Query Frame = 0


Query: 1   MATTSSTHLHLHLPLLPLPQPHHRTFLP--LPTTLLRRNLRSLTVSVGTDILGDFGARDP 60
           MATT +     + PLL LP+PH R  LP    TTLLRR LR  T+       GDFGARDP
Sbjct: 1   MATTPTA----NFPLLRLPRPHSRFSLPPSPSTTLLRRKLRPATIRAAVVGDGDFGARDP 60

Query: 61  FPEEIASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKKLLRKV 120
           FP EI SNFG+KVIGFS+TEHKILIPN RALALS+Q+CVP+SSLQAPM EDEA+KLL+KV
Sbjct: 61  FPAEIESNFGDKVIGFSNTEHKILIPNVRALALSQQQCVPISSLQAPMPEDEAQKLLKKV 120

Query: 121 VGWKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQVRAELW 180
           VGW+LVD GRG+ KL+CLWKLRDFKC VELINRI   V++  HFPD+HL+QPNQV AELW
Sbjct: 121 VGWRLVDNGRGQFKLRCLWKLRDFKCCVELINRISNAVDDANHFPDIHLDQPNQVTAELW 180

Query: 181 TSSIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 213
           TS+IGGLSMND+IVAAKIDEIKT DLA RKRAWA
Sbjct: 181 TSAIGGLSMNDFIVAAKIDEIKTIDLAARKRAWA 210

[Close]

BLAST of CsaV3_5G004930 vs. ExPASy TrEMBL
Match: A0A6J1H2N5 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111459142 PE=3 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 2.4e-79
Identity = 149/209 (71.29%), Postives = 172/209 (82.30%), Query Frame = 0


Query: 6   STHLHLHLPLLPLPQPHHRTFLPL--PTTLLRRNLRSLTVSVGTDILGDFGARDPFPEEI 65
           +T  + + PLL LP+PH R  LP   P+T LRR LR  T+       GDFGARDPFP EI
Sbjct: 2   ATAPNANFPLLRLPRPHSRFSLPQTPPSTFLRRKLRPATIRAAVVGDGDFGARDPFPAEI 61

Query: 66  ASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKKLLRKVVGWKL 125
            SNFG+KVIGFS+TEHKILIPN RALALS+Q+CVP+SSLQAPM EDEA+KLL+KVVGW+L
Sbjct: 62  ESNFGDKVIGFSNTEHKILIPNVRALALSQQQCVPISSLQAPMPEDEAQKLLKKVVGWRL 121

Query: 126 VDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQVRAELWTSSIG 185
           VD GRG+ KL+CLWKLRDFKC VELINRI   V++  HFPD+HL+QPNQV AELWTS+IG
Sbjct: 122 VDNGRGQFKLRCLWKLRDFKCCVELINRISNAVDDANHFPDIHLDQPNQVTAELWTSAIG 181

Query: 186 GLSMNDYIVAAKIDEIKTSDLAPRKRAWA 213
           GLSMND+I+AAKIDEIKT DLA RKRAWA
Sbjct: 182 GLSMNDFIIAAKIDEIKTIDLAARKRAWA 210

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BLAST of CsaV3_5G004930 vs. TAIR 10
Match: AT5G51110.1 (Transcriptional coactivator/pterin dehydratase )

HSP 1 Score: 251.1 bits (640), Expect = 7.8e-67
Identity = 131/219 (59.82%), Postives = 161/219 (73.52%), Query Frame = 0


Query: 2   ATTSSTHLHLHLPLLPLPQPHHRTFLPL-----PTTLLRRNLRSLTV---SVGTDILGDF 61
           AT+SS   ++    L L QP  R+ L +     P +   R L  LTV   ++  D LGDF
Sbjct: 3   ATSSSPPCNISASSLLLRQP-SRSILKVFGLLPPVSRNNRKLGRLTVTRSNLAQDFLGDF 62

Query: 62  GARDPFPEEIASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKK 121
           GARDP+PEEIAS FG+KV+G   TEHKILIPNA  L+LS+ +C PVSS Q P++ D+A+ 
Sbjct: 63  GARDPYPEEIASQFGDKVLGCQSTEHKILIPNASVLSLSQLQCSPVSSSQPPLSGDDART 122

Query: 122 LLRKVVGWKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQV 181
           LL KV+GW +VD   G LK++C+WK+RDF C VELINRI KV E  GH+P LHLE P QV
Sbjct: 123 LLHKVLGWSIVDNEAGGLKIRCMWKVRDFGCGVELINRIHKVAEASGHYPSLHLESPTQV 182

Query: 182 RAELWTSSIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 213
           RAEL+TSSIGGLSMND+I+AAKID+IKTSDL+PRKRAWA
Sbjct: 183 RAELFTSSIGGLSMNDFIMAAKIDDIKTSDLSPRKRAWA 220

[Close]

BLAST of CsaV3_5G004930 vs. TAIR 10
Match: AT5G51110.2 (Transcriptional coactivator/pterin dehydratase )

HSP 1 Score: 250.8 bits (639), Expect = 1.0e-66
Identity = 118/175 (67.43%), Postives = 142/175 (81.14%), Query Frame = 0


Query: 38  LRSLTVSVGTDILGDFGARDPFPEEIASNFGEKVIGFSDTEHKILIPNARALALSEQECV 97
           LR  + S+  D LGDFGARDP+PEEIAS FG+KV+G   TEHKILIPNA  L+LS+ +C 
Sbjct: 19  LRQPSRSILKDFLGDFGARDPYPEEIASQFGDKVLGCQSTEHKILIPNASVLSLSQLQCS 78

Query: 98  PVSSLQAPMAEDEAKKLLRKVVGWKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVE 157
           PVSS Q P++ D+A+ LL KV+GW +VD   G LK++C+WK+RDF C VELINRI KV E
Sbjct: 79  PVSSSQPPLSGDDARTLLHKVLGWSIVDNEAGGLKIRCMWKVRDFGCGVELINRIHKVAE 138

Query: 158 NVGHFPDLHLEQPNQVRAELWTSSIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 213
             GH+P LHLE P QVRAEL+TSSIGGLSMND+I+AAKID+IKTSDL+PRKRAWA
Sbjct: 139 ASGHYPSLHLESPTQVRAELFTSSIGGLSMNDFIMAAKIDDIKTSDLSPRKRAWA 193

[Close]

BLAST of CsaV3_5G004930 vs. TAIR 10
Match: AT1G29810.1 (Transcriptional coactivator/pterin dehydratase )

HSP 1 Score: 101.7 bits (252), Expect = 7.6e-22
Identity = 49/120 (40.83%), Postives = 75/120 (62.50%), Query Frame = 0


Query: 91  LSEQECVPVSSLQ-APMAEDEAKKLLRKVVGWKLVDGGRGELKLQCLWKLRDFKCSVELI 150
           LS ++CVP ++     M E  A+ LL+KV GW L +     LKL   W+++ F   ++  
Sbjct: 66  LSTKKCVPCNAKDLRAMTEQSAQDLLQKVAGWDLAN-DNDTLKLHRSWRVKSFTKGLDFF 125

Query: 151 NRICKVVENVGHFPDLHLEQPNQVRAELWTSSIGGLSMNDYIVAAKIDEIKTSDLAPRKR 210
            R+  + E+ GH PDLHL   N V+ E+WT +IGGL+ ND+I+AAKI+E++  DL  +K+
Sbjct: 126 QRVADIAESEGHHPDLHLVGWNNVKIEIWTHAIGGLTENDFILAAKINELQVEDLLRKKK 184

The following BLAST results are available for this feature:

BLAST of CsaV3_5G004930 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143676.1	3.4e-117	100.00	probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic [Cucumis sativu...	[more]
XP_008436971.1	8.8e-105	90.57	PREDICTED: putative pterin-4-alpha-carbinolamine dehydratase [Cucumis melo] >KAA...	[more]
XP_038877111.1	2.7e-93	84.11	probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic [Benincasa hisp...	[more]
XP_022996033.1	9.8e-80	71.96	uncharacterized protein LOC111491359 [Cucurbita maxima] >XP_023532646.1 uncharac...	[more]
XP_022957679.1	4.9e-79	71.29	uncharacterized protein LOC111459142 [Cucurbita moschata] >KAG7035558.1 hypothet...	[more]

BLAST of CsaV3_5G004930 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LU63	1.1e-65	59.82	Probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic OS=Arabidopsis ...	[more]
Q6QJ72	1.1e-20	40.83	Pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial OS=Arabidopsis thalian...	[more]
B0JQV0	1.2e-11	33.94	Putative pterin-4-alpha-carbinolamine dehydratase OS=Microcystis aeruginosa (str...	[more]
Q60BH8	1.1e-09	32.14	Putative pterin-4-alpha-carbinolamine dehydratase OS=Methylococcus capsulatus (s...	[more]
B9LPE3	2.5e-09	30.70	Putative pterin-4-alpha-carbinolamine dehydratase OS=Halorubrum lacusprofundi (s...	[more]

BLAST of CsaV3_5G004930 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KL10	1.7e-117	100.00	4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis sativus OX=3659 GN=Csa_5G16...	[more]
A0A5A7UGL0	4.3e-105	90.57	4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3ASJ9	4.3e-105	90.57	4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis melo OX=3656 GN=LOC10348254...	[more]
A0A6J1K9P8	4.7e-80	71.96	4a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1H2N5	2.4e-79	71.29	4a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

BLAST of CsaV3_5G004930 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G51110.1	7.8e-67	59.82	Transcriptional coactivator/pterin dehydratase	[more]
AT5G51110.2	1.0e-66	67.43	Transcriptional coactivator/pterin dehydratase	[more]
AT1G29810.1	7.6e-22	40.83	Transcriptional coactivator/pterin dehydratase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036428	Pterin 4 alpha carbinolamine dehydratase superfamily	GENE3D	3.30.1360.20	Transcriptional coactivator/pterin dehydratase	coord: 105..204 e-value: 6.6E-21 score: 76.0
IPR036428	Pterin 4 alpha carbinolamine dehydratase superfamily	SUPERFAMILY	55248	PCD-like	coord: 104..199
IPR001533	Pterin 4 alpha carbinolamine dehydratase	PFAM	PF01329	Pterin_4a	coord: 105..199 e-value: 2.3E-20 score: 72.5
IPR001533	Pterin 4 alpha carbinolamine dehydratase	PANTHER	PTHR12599	PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE	coord: 33..212
None	No IPR available	PANTHER	PTHR12599:SF8	PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE, CHLOROPLASTIC-RELATED	coord: 33..212

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_5G004930.1	CsaV3_5G004930.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006729	tetrahydrobiopterin biosynthetic process
molecular_function	GO:0008124	4-alpha-hydroxytetrahydrobiopterin dehydratase activity