CsaV3_5G002300 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_5G002300
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionMannan endo-1,4-beta-mannosidase
Locationchr5: 1405040 .. 1409109 (+)
RNA-Seq ExpressionCsaV3_5G002300
SyntenyCsaV3_5G002300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAAAAAGATTGTATTCTATTTAATTTCTTTAATTTTCTTCTTCTCTTTATTGGTTCTTTCACAATCCTTACCTCTTTCCACAAGAGGAAGATGGATTGTAGACTCTAAAACAGGCCAACGTGTGAAGCTAAGCTGTGTTAATTTGGCCTCTCATGCACAATCCATGTTGGCACAAGGTCTTGACAAACGAACATTGAAGGATTTAGCTAATGATATCATTAGTCGCGATTTTAACTGTGTACGCTTGACTTGGTCGACTCATATGTTCACTCGCTACTCCCACATGACTATCATTGATGTTCTAGACAATCTTGATATTCAAAATGTCAAAAGTGGAGTTCAAAAACACAACCCTAAGATGTTGAATATGACAGTGGATCATGCATTCAAAACTGTTGTCTATGGCTTGGGCAATGCAGGTCTCATGATTGTTTTGGACAACCACATTAGCCAGCCGAGATGGTGTTGCTCTCTCGATGATGGCAATGGCTTCTTTGGAGACCGTCATTTTGACACTCTTGAATGGCTTCAGGGCTTGGACTATGTTGCTAGACATTTCACTTGGCATTCTCAAGTGAGTTGGTTTTTTATCTTTTTAGGTCAGCATGCATATATGTGTTTGTTCATTTGTTAACAAATCATATTCTAAATTCTTTCATTTTAATTGGTATGTATAGGTGATAGGAATGAGCCTAAGAAACGAACTCCGAGGGCCATATACAAACATGGACAATTGGTACAAATACGTGAAGGAAGGAAGCCACTTGATCCACACAAAAAACCGTAAACTCCTAGTAATAATTTCAGGTATAACCTTCGATAACGATCTAAGCTTCCTAAAGAAAAAGTCATTAGGGTACAACTTGCACAATAAAGTAGTGTTGGAGGCACATTTGTACCCGTTCAGTGGGAGTGAGAAATTACCCGAAAGCAAGTTTGTGAAGAAGCCATTAAACATAGTATGCAACCAAGTGATGGAAAAATTCGAGAGAGAAGCTGGATTTGTGGTGAACATGGAGGATGAACCATATCCATTGTGGCTTAGTGAATTTGGGTACGATCTAAGGGGTGACAACAAGGCTCAAAACAGGTTCATGAGTTGCTTTTTGGCTCATATTGTTGCCAAGGATCTTGATTGGGCTTATTGGGCTTTTCAAGGAACTTACATGTATAGACAAGGTCAAGAAAGTGTCGATGAAACTTTTGGTGTCATGGATAGCACTTGGACTAATGATCGAAACCCTCAACTCAATCAAATGCTTCAACTTGCTAAAAGAATCAACCAAGGTTATGTTTGTTCTAACCCTACTACTCACCCGTGTATCCAATTTTCTAACCCTGATCATCTCATATTCTTTATTTTTTTTCTATCCTTTTAATTTTCTGTTTTTGTAGATCCAAATTCGAATGCATCAATGTCATACATAATATTACATCCAGTATCGGGACAATGTATCAGATCAAATGGTCAAGGAGGAATTGTACTTGGTGATTGCTTGACGCCAACACACTGGACCCACAGCGGAGATGGAAGTTCAATGAAGTTGTCGAACGGGCAATGTTTGCAGTCGGCTGGAGATGGCAAACCCCTCATTGTCTCAGCCGAATGCTCCAGTGACGGAAGCAAATGGACGATGGCTTCAAAGGCTAAGCTTCAATTGGCCACCAAAAGCGGTGGAGAAAACTTTTGTTTAGAGAAGAAATCTAATACTAAAATTGTTGTGAAGAGATGCATTTGCCTTGAAGATGAGTTGAATTGCTTTAACGATCCTCAGCCCCAGTGGTTCAAGCTTGTTCCAACAAATGTTGCTTAGTTTATTAATTAGTTCTATTTATAATTTTATATATTTTTTTCCAAGGTTATCATGTCAACAATAATAATTCTAATTTTTGTAATTATATCCCATCTTTATAGATCCCAAAAATCTCTACATCTATATAAGAATCAACTGATGAAATATAAAATATAAACCTTTTGTATCTTTTTTCCTAATTTTGTGTAATAAGATTTTTAAAATTAAGCTTGGTTTATAAATTTTCTTGTTCTGTTTTTTCAAAAAATGAATTTGAAATTATAATTCTCTTAGGAAAAAAAATTTAGAGACAACTCGTATGTGTGTGTTTTTAAAATTAAAATCAGAGAGATATATAAATCAATTATTGTTGGTCTACCTTCTTTAATTAATTTATTGTTTTTTCTTTAATTTTTAAGAGGGTATGTTGATAATTTTCTGATAAATAATACATGTTTAGAAAAATAAAACCATCAATATATAAATTAGAAAAGTTAGTACATCATGGGGGTGTAAATAAACTAATATAAATTAGTAATTTCAACTAATTGATATCTATTATTATTTATTTGTTCTTTTTCTAATACAAAATCAATATCATGCCTTAGAGAATCATATTCTGTTCGCTAATGTTGATACTAAATTAGGAATATTTATTCCACACATATCATATCTAATTTAATTCTAGTTTTCCTTTTCAAGATGCATCCCTGCATGTGGCCCATATGTCAATTCTTTTGATATACTGGGGGCGGTTGAACAGAAAAAAAAAATAGTTAAAAAAAAGGGAAACAATGTTAAATAAATTAATTCTACATCGGGCGATATGTAATTAATTTAATTATATGAACCAGAGTACACTTTTTTATTTTAATTATTTTCATATTGTCAATAAGTTTATAGTTTATTAATATGTATTTTTAAAAATAATTATTTGATTATTATTACCTTTCAACTAAGATGAAAACATGTGCATGAAAATATATCAATATGAAAGAATAATTATTACCATATATTGTTAAATTAATATTTTTTATATTATTTGTTGCAGTTTAATTAAAATCATGAATATAATCCAATTGATGAAATCAACTCTGTAAAGGCTTTGTAATTATTTTCTGTTGGTTTTCTCATTACTTTGTGTGTTTGCAGTATAAATTCAATTTCATCATATAGTTCTCTCTAAATAAAATATTGAACTTTGCATAGATCTAATATTAGTGAAAGTGATAATAATTAGCATTGTAAAATATGTGCTAACAACCCTAGAGCCATACTATTTAATACTTGGGAACATACTAGCTAATTCATCATGCTTCTATATAAATAGTTTGAGATTTAATTAACTTATTAATTCTTTAGAATTAACTACTTTACATGGTTCTTATGAAGGAGTCTATTGTAAAGAAATGATGGGAAAACTAAAACTGCATAATTTCAAGAACCAAACAAAAAAAAAAAGTTATCAAGTGAGATTCTAAACCCAAGATATCAAAATACTAAGAAAGGAAAATAAACACAAGAACGATCCATGGAAGAAAAATTTAGGAAGAGAAACATTTACTTCTTAATGTTTGTTAATTAAACTTTGAAATTTTAATTTTAATTTAGATCCTATACATCACCATTTTCTTAAAAATAGAATTGGGCACATAATAAGGATGAGAAAATTTGTTATTCACAAAATTATGTAATAAGATATTCACATTAACTAATAACATATCAGACAACCTTCGACTCTAACCTCTCTATATGCTTCATGCCAAAATCCCTAAAAAACTAGAAGAAAAAAACTATTAACTAAATATCTTAAACTAATTCTCAATTATTCCCTTTTAAATTAACAATCTAATTTCCTAAATAAAACTAATTACTAAACTAAGCAACATTTGTTGGAACAAGCTTGAACCACTGGCTCTGAGGATCATCGAAGCAATTCCACTCATCTTTAAGGCAAATGCATTTCTTCACAACAATTGAAGTATCAGATTCCTTCTCTAAGCAAATGTTTTCTCCCCCACTTTTGGTGGACAATTGAAGCTTTGCCTTAGAAGCCACCGTCCAACTGCTTCCGTCACCAGAGCATTCGGCCGACACCACAGGCGGCTTCCCATCTCCGGCTGACTTCAAACACTGCCCGTTCCACAACTTCATCGGACTTCCATCTCCAGTATGGTCCCACAACGTCGGTGTCTCACAGTCACCTAGTTCAATTCCTCCCTTGCCATCTAGTTTGACACATTGCCCTGACACTGGATGTAGCATTATGTAG

mRNA sequence

ATGGCTAAAAAGATTGTATTCTATTTAATTTCTTTAATTTTCTTCTTCTCTTTATTGGTTCTTTCACAATCCTTACCTCTTTCCACAAGAGGAAGATGGATTGTAGACTCTAAAACAGGCCAACGTGTGAAGCTAAGCTGTGTTAATTTGGCCTCTCATGCACAATCCATGTTGGCACAAGGTCTTGACAAACGAACATTGAAGGATTTAGCTAATGATATCATTAGTCGCGATTTTAACTGTGTACGCTTGACTTGGTCGACTCATATGTTCACTCGCTACTCCCACATGACTATCATTGATGTTCTAGACAATCTTGATATTCAAAATGTCAAAAGTGGAGTTCAAAAACACAACCCTAAGATGTTGAATATGACAGTGGATCATGCATTCAAAACTGTTGTCTATGGCTTGGGCAATGCAGGTCTCATGATTGTTTTGGACAACCACATTAGCCAGCCGAGATGGTGTTGCTCTCTCGATGATGGCAATGGCTTCTTTGGAGACCGTCATTTTGACACTCTTGAATGGCTTCAGGGCTTGGACTATGTTGCTAGACATTTCACTTGGCATTCTCAAGTGATAGGAATGAGCCTAAGAAACGAACTCCGAGGGCCATATACAAACATGGACAATTGGTACAAATACGTGAAGGAAGGAAGCCACTTGATCCACACAAAAAACCGTAAACTCCTAGTAATAATTTCAGGTATAACCTTCGATAACGATCTAAGCTTCCTAAAGAAAAAGTCATTAGGGTACAACTTGCACAATAAAGTAGTGTTGGAGGCACATTTGTACCCGTTCAGTGGGAGTGAGAAATTACCCGAAAGCAAGTTTGTGAAGAAGCCATTAAACATAGTATGCAACCAAGTGATGGAAAAATTCGAGAGAGAAGCTGGATTTGTGGTGAACATGGAGGATGAACCATATCCATTGTGGCTTAGTGAATTTGGGTACGATCTAAGGGGTGACAACAAGGCTCAAAACAGGTTCATGAGTTGCTTTTTGGCTCATATTGTTGCCAAGGATCTTGATTGGGCTTATTGGGCTTTTCAAGGAACTTACATGTATAGACAAGGTCAAGAAAGTGTCGATGAAACTTTTGGTGTCATGGATAGCACTTGGACTAATGATCGAAACCCTCAACTCAATCAAATGCTTCAACTTGCTAAAAGAATCAACCAAGATCCAAATTCGAATGCATCAATGTCATACATAATATTACATCCAGTATCGGGACAATGTATCAGATCAAATGGTCAAGGAGGAATTGTACTTGGTGATTGCTTGACGCCAACACACTGGACCCACAGCGGAGATGGAAGTTCAATGAAGTTGTCGAACGGGCAATGTTTGCAGTCGGCTGGAGATGGCAAACCCCTCATTGTCTCAGCCGAATGCTCCAGTGACGGAAGCAAATGGACGATGGCTTCAAAGGCTAAGCTTCAATTGGCCACCAAAAGCGGTGGAGAAAACTTTTGTTTAGAGAAGAAATCTAATACTAAAATTGTTGTGAAGAGATGCATTTGCCTTGAAGATGAGTTGAATTGCTTTAACGATCCTCAGCCCCACTTTGCCTTAGAAGCCACCGTCCAACTGCTTCCGTCACCAGAGCATTCGGCCGACACCACAGGCGGCTTCCCATCTCCGGCTGACTTCAAACACTGCCCGTTCCACAACTTCATCGGACTTCCATCTCCAGTATGGTCCCACAACGTCGGTGTCTCACAGTCACCTAGTTCAATTCCTCCCTTGCCATCTAGTTTGACACATTGCCCTGACACTGGATGTAGCATTATGTAG

Coding sequence (CDS)

ATGGCTAAAAAGATTGTATTCTATTTAATTTCTTTAATTTTCTTCTTCTCTTTATTGGTTCTTTCACAATCCTTACCTCTTTCCACAAGAGGAAGATGGATTGTAGACTCTAAAACAGGCCAACGTGTGAAGCTAAGCTGTGTTAATTTGGCCTCTCATGCACAATCCATGTTGGCACAAGGTCTTGACAAACGAACATTGAAGGATTTAGCTAATGATATCATTAGTCGCGATTTTAACTGTGTACGCTTGACTTGGTCGACTCATATGTTCACTCGCTACTCCCACATGACTATCATTGATGTTCTAGACAATCTTGATATTCAAAATGTCAAAAGTGGAGTTCAAAAACACAACCCTAAGATGTTGAATATGACAGTGGATCATGCATTCAAAACTGTTGTCTATGGCTTGGGCAATGCAGGTCTCATGATTGTTTTGGACAACCACATTAGCCAGCCGAGATGGTGTTGCTCTCTCGATGATGGCAATGGCTTCTTTGGAGACCGTCATTTTGACACTCTTGAATGGCTTCAGGGCTTGGACTATGTTGCTAGACATTTCACTTGGCATTCTCAAGTGATAGGAATGAGCCTAAGAAACGAACTCCGAGGGCCATATACAAACATGGACAATTGGTACAAATACGTGAAGGAAGGAAGCCACTTGATCCACACAAAAAACCGTAAACTCCTAGTAATAATTTCAGGTATAACCTTCGATAACGATCTAAGCTTCCTAAAGAAAAAGTCATTAGGGTACAACTTGCACAATAAAGTAGTGTTGGAGGCACATTTGTACCCGTTCAGTGGGAGTGAGAAATTACCCGAAAGCAAGTTTGTGAAGAAGCCATTAAACATAGTATGCAACCAAGTGATGGAAAAATTCGAGAGAGAAGCTGGATTTGTGGTGAACATGGAGGATGAACCATATCCATTGTGGCTTAGTGAATTTGGGTACGATCTAAGGGGTGACAACAAGGCTCAAAACAGGTTCATGAGTTGCTTTTTGGCTCATATTGTTGCCAAGGATCTTGATTGGGCTTATTGGGCTTTTCAAGGAACTTACATGTATAGACAAGGTCAAGAAAGTGTCGATGAAACTTTTGGTGTCATGGATAGCACTTGGACTAATGATCGAAACCCTCAACTCAATCAAATGCTTCAACTTGCTAAAAGAATCAACCAAGATCCAAATTCGAATGCATCAATGTCATACATAATATTACATCCAGTATCGGGACAATGTATCAGATCAAATGGTCAAGGAGGAATTGTACTTGGTGATTGCTTGACGCCAACACACTGGACCCACAGCGGAGATGGAAGTTCAATGAAGTTGTCGAACGGGCAATGTTTGCAGTCGGCTGGAGATGGCAAACCCCTCATTGTCTCAGCCGAATGCTCCAGTGACGGAAGCAAATGGACGATGGCTTCAAAGGCTAAGCTTCAATTGGCCACCAAAAGCGGTGGAGAAAACTTTTGTTTAGAGAAGAAATCTAATACTAAAATTGTTGTGAAGAGATGCATTTGCCTTGAAGATGAGTTGAATTGCTTTAACGATCCTCAGCCCCACTTTGCCTTAGAAGCCACCGTCCAACTGCTTCCGTCACCAGAGCATTCGGCCGACACCACAGGCGGCTTCCCATCTCCGGCTGACTTCAAACACTGCCCGTTCCACAACTTCATCGGACTTCCATCTCCAGTATGGTCCCACAACGTCGGTGTCTCACAGTCACCTAGTTCAATTCCTCCCTTGCCATCTAGTTTGACACATTGCCCTGACACTGGATGTAGCATTATGTAG

Protein sequence

MAKKIVFYLISLIFFFSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLASHAQSMLAQGLDKRTLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIIDVLDNLDIQNVKSGVQKHNPKMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLEWLQGLDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIISGITFDNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKFEREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTYMYRQGQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQCIRSNGQGGIVLGDCLTPTHWTHSGDGSSMKLSNGQCLQSAGDGKPLIVSAECSSDGSKWTMASKAKLQLATKSGGENFCLEKKSNTKIVVKRCICLEDELNCFNDPQPHFALEATVQLLPSPEHSADTTGGFPSPADFKHCPFHNFIGLPSPVWSHNVGVSQSPSSIPPLPSSLTHCPDTGCSIM*
Homology
BLAST of CsaV3_5G002300 vs. NCBI nr
Match: KAE8647841.1 (hypothetical protein Csa_000559 [Cucumis sativus])

HSP 1 Score: 1250.3 bits (3234), Expect = 0.0e+00
Identity = 601/601 (100.00%), Postives = 601/601 (100.00%), Query Frame = 0

Query: 1   MAKKIVFYLISLIFFFSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLASHAQSMLAQ 60
           MAKKIVFYLISLIFFFSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLASHAQSMLAQ
Sbjct: 1   MAKKIVFYLISLIFFFSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLASHAQSMLAQ 60

Query: 61  GLDKRTLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIIDVLDNLDIQNVKSGVQKHNP 120
           GLDKRTLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIIDVLDNLDIQNVKSGVQKHNP
Sbjct: 61  GLDKRTLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIIDVLDNLDIQNVKSGVQKHNP 120

Query: 121 KMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLEWLQG 180
           KMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLEWLQG
Sbjct: 121 KMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLEWLQG 180

Query: 181 LDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIISGITF 240
           LDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIISGITF
Sbjct: 181 LDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIISGITF 240

Query: 241 DNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKFEREA 300
           DNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKFEREA
Sbjct: 241 DNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKFEREA 300

Query: 301 GFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTYMYRQ 360
           GFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTYMYRQ
Sbjct: 301 GFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTYMYRQ 360

Query: 361 GQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQCIRSN 420
           GQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQCIRSN
Sbjct: 361 GQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQCIRSN 420

Query: 421 GQGGIVLGDCLTPTHWTHSGDGSSMKLSNGQCLQSAGDGKPLIVSAECSSDGSKWTMASK 480
           GQGGIVLGDCLTPTHWTHSGDGSSMKLSNGQCLQSAGDGKPLIVSAECSSDGSKWTMASK
Sbjct: 421 GQGGIVLGDCLTPTHWTHSGDGSSMKLSNGQCLQSAGDGKPLIVSAECSSDGSKWTMASK 480

Query: 481 AKLQLATKSGGENFCLEKKSNTKIVVKRCICLEDELNCFNDPQPHFALEATVQLLPSPEH 540
           AKLQLATKSGGENFCLEKKSNTKIVVKRCICLEDELNCFNDPQPHFALEATVQLLPSPEH
Sbjct: 481 AKLQLATKSGGENFCLEKKSNTKIVVKRCICLEDELNCFNDPQPHFALEATVQLLPSPEH 540

Query: 541 SADTTGGFPSPADFKHCPFHNFIGLPSPVWSHNVGVSQSPSSIPPLPSSLTHCPDTGCSI 600
           SADTTGGFPSPADFKHCPFHNFIGLPSPVWSHNVGVSQSPSSIPPLPSSLTHCPDTGCSI
Sbjct: 541 SADTTGGFPSPADFKHCPFHNFIGLPSPVWSHNVGVSQSPSSIPPLPSSLTHCPDTGCSI 600

Query: 601 M 602
           M
Sbjct: 601 M 601

BLAST of CsaV3_5G002300 vs. NCBI nr
Match: XP_004143962.1 (glycosyl hydrolase 5 family protein [Cucumis sativus])

HSP 1 Score: 983.8 bits (2542), Expect = 6.6e-283
Identity = 468/470 (99.57%), Postives = 469/470 (99.79%), Query Frame = 0

Query: 57  MLAQGLDKRTLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIIDVLDNLDIQNVKSGVQ 116
           MLAQGLDKRTLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIIDVLDNLDIQNVKSGVQ
Sbjct: 1   MLAQGLDKRTLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIIDVLDNLDIQNVKSGVQ 60

Query: 117 KHNPKMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLE 176
           KHNPKMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLE
Sbjct: 61  KHNPKMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLE 120

Query: 177 WLQGLDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIIS 236
           WLQGLDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIIS
Sbjct: 121 WLQGLDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIIS 180

Query: 237 GITFDNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKF 296
           GITFDNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKF
Sbjct: 181 GITFDNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKF 240

Query: 297 EREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTY 356
           EREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTY
Sbjct: 241 EREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTY 300

Query: 357 MYRQGQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQC 416
           MYRQGQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQC
Sbjct: 301 MYRQGQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQC 360

Query: 417 IRSNGQGGIVLGDCLTPTHWTHSGDGSSMKLSNGQCLQSAGDGKPLIVSAECSSDGSKWT 476
           IRSNGQGGIVLGDCLTPTHWTHSGDGSSMKLSNGQCLQSAGDGKPLIVSAECSSDGSKWT
Sbjct: 361 IRSNGQGGIVLGDCLTPTHWTHSGDGSSMKLSNGQCLQSAGDGKPLIVSAECSSDGSKWT 420

Query: 477 MASKAKLQLATKSGGENFCLEKKSNTKIVVKRCICLEDELNCFNDPQPHF 527
           MASKAKLQLATKSGGENFCLEKKSNTKIVVKRCICLEDELNCFNDPQP +
Sbjct: 421 MASKAKLQLATKSGGENFCLEKKSNTKIVVKRCICLEDELNCFNDPQPQW 470

BLAST of CsaV3_5G002300 vs. NCBI nr
Match: XP_008437728.1 (PREDICTED: uncharacterized protein LOC103483074 [Cucumis melo] >KAA0042707.1 major extracellular endoglucanase-like [Cucumis melo var. makuwa] >TYK06111.1 major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 906.0 bits (2340), Expect = 1.8e-259
Identity = 431/471 (91.51%), Postives = 447/471 (94.90%), Query Frame = 0

Query: 57  MLAQGLDKRTLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIIDVLDNLDIQNVKSGVQ 116
           MLAQGLDKR LKDLANDIISRDFNCVRLTWSTHMFTRYSHMTI+DVLD+LDIQNVKSGVQ
Sbjct: 1   MLAQGLDKRPLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIVDVLDDLDIQNVKSGVQ 60

Query: 117 KHNPKMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLE 176
            HNP+MLNMTV HAFKTVV GLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFD LE
Sbjct: 61  THNPEMLNMTVAHAFKTVVDGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDALE 120

Query: 177 WLQGLDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIIS 236
           WLQGLDYVAR+FT HSQVIGMSLRNELRGPYTNMDNWYKYV  GSHLIHTKN  LLVIIS
Sbjct: 121 WLQGLDYVARNFTGHSQVIGMSLRNELRGPYTNMDNWYKYVSNGSHLIHTKNPNLLVIIS 180

Query: 237 GITFDNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKF 296
           G+TFD DLSFLK+K LGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLN+VCNQV EKF
Sbjct: 181 GVTFDTDLSFLKEKPLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNVVCNQVKEKF 240

Query: 297 EREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTY 356
           EREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIV KDLDWAYWAFQGTY
Sbjct: 241 EREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVGKDLDWAYWAFQGTY 300

Query: 357 MYRQGQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQC 416
           MYRQGQESVDE FG+MDSTWT DRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQC
Sbjct: 301 MYRQGQESVDEAFGIMDSTWTKDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQC 360

Query: 417 IRSNGQGGIVLGDCLTPTHWTHSGDGSSMK-LSNGQCLQSAGDGKPLIVSAECSSDGSKW 476
           IRSNGQGGI LGDCLTPT WT+SGDGSSMK LSNGQCL+SAGDGKP +VS ECS + SKW
Sbjct: 361 IRSNGQGGIALGDCLTPTQWTYSGDGSSMKLLSNGQCLKSAGDGKPPVVSTECSGEESKW 420

Query: 477 TMASKAKLQLATKSGGENFCLEKKSNTKIVVKRCICLEDELNCFNDPQPHF 527
           TMASKAKLQLATKSGGE+FCLEKKS+TKIVVKRCICL+DELNCFNDPQP +
Sbjct: 421 TMASKAKLQLATKSGGEDFCLEKKSDTKIVVKRCICLKDELNCFNDPQPQW 471

BLAST of CsaV3_5G002300 vs. NCBI nr
Match: XP_038907078.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 866.7 bits (2238), Expect = 1.2e-247
Identity = 419/523 (80.11%), Postives = 466/523 (89.10%), Query Frame = 0

Query: 1   MAKKIVFYLISLIFFFSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLASHAQSMLAQ 60
           MAK   F LISL+ FFSLL LSQSL LSTRGRWIVDS+TGQRVKLSCVNL SH QSML Q
Sbjct: 1   MAK--AFSLISLV-FFSLLPLSQSLTLSTRGRWIVDSQTGQRVKLSCVNLVSHGQSMLTQ 60

Query: 61  GLDKRTLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIIDVLDNLDIQNVKSGVQKHNP 120
           GLDKR L DLANDIISRDFNCVRLTWS HMFTRYS MTI+DVLDNLDI+NVKSGVQ HN 
Sbjct: 61  GLDKRPLNDLANDIISRDFNCVRLTWSIHMFTRYSQMTIVDVLDNLDIKNVKSGVQTHNL 120

Query: 121 KMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLEWLQG 180
           +MLNMTV HAFKTV+ GLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHF T+EWL+G
Sbjct: 121 EMLNMTVAHAFKTVIDGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFHTVEWLRG 180

Query: 181 LDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIISGITF 240
           L YVARH++WH QVIGMSLRNELRGP+TNMD WYKYV++GSHLIH +N  LLV+ISGI +
Sbjct: 181 LTYVARHYSWHPQVIGMSLRNELRGPFTNMDKWYKYVRKGSHLIHRENPNLLVMISGINY 240

Query: 241 DNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKFEREA 300
           DNDLSFLKKK LGYNL+NKVVLEAHLY FS   + PESKF++KPLN+ C+ VME+FEREA
Sbjct: 241 DNDLSFLKKKPLGYNLNNKVVLEAHLYSFS---EEPESKFLEKPLNVACDHVMERFEREA 300

Query: 301 GFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTYMYRQ 360
           GFVV+ME+ PYPLWLSEFGY+  G N+AQNRFMSCFLA I+ KDLDWA WAFQGTYMYRQ
Sbjct: 301 GFVVDMEN-PYPLWLSEFGYNQGGANEAQNRFMSCFLARIIGKDLDWALWAFQGTYMYRQ 360

Query: 361 GQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQCIRSN 420
           GQE+ +ETFGVMDS+WT DRNPQL QMLQLAKRIN DPNSN  M YI+LHPVSGQC+RS+
Sbjct: 361 GQENAEETFGVMDSSWTKDRNPQLKQMLQLAKRINLDPNSNEPMLYIMLHPVSGQCVRSD 420

Query: 421 GQGGIVLGDCLTPTHWTHSGDGSSMKLSNGQCLQSAGDGKPLIVSAECSSDGSKWTMASK 480
            +GGIVLGDCLTPT W+HSGDGS ++LS G CL+S GDGKP +VSA+CS DGS WT+ASK
Sbjct: 421 NEGGIVLGDCLTPTRWSHSGDGSLLQLSEGLCLKSVGDGKPPVVSADCSGDGSIWTVASK 480

Query: 481 AKLQLATKSGGENFCLEKKSNTKIVVKRCICLEDELNCFNDPQ 524
           AKLQLATKSGGENFCLEKKS++KIVVK+CICL+DELNCF+DPQ
Sbjct: 481 AKLQLATKSGGENFCLEKKSDSKIVVKKCICLKDELNCFDDPQ 516

BLAST of CsaV3_5G002300 vs. NCBI nr
Match: XP_038907081.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 841.3 bits (2172), Expect = 5.3e-240
Identity = 409/516 (79.26%), Postives = 456/516 (88.37%), Query Frame = 0

Query: 9   LISLIFFFSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLASHAQSMLAQGLDKRTLK 68
           LISL+  FSLLVLSQSLPLST GRWIVDS+TGQRVKLSCVNL SHAQSMLAQGLDKR+LK
Sbjct: 7   LISLV-LFSLLVLSQSLPLSTSGRWIVDSQTGQRVKLSCVNLVSHAQSMLAQGLDKRSLK 66

Query: 69  DLANDIISRDFNCVRLTWSTHMFTRYSHMTIIDVLDNLDIQNVKSGVQKHNPKMLNMTVD 128
           DLANDI SRDFNCVRLTWS HMFTR+++ TI DVLD LDI  VKSGVQKHNP++LNMTV 
Sbjct: 67  DLANDISSRDFNCVRLTWSIHMFTRHAYETIGDVLDGLDIGKVKSGVQKHNPEILNMTVS 126

Query: 129 HAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLEWLQGLDYVARHF 188
           HAF+TV+ GLGNAGLMIVLDNHISQPRWCCSL+DGNGFFGDR+F+  EWLQGL YVARHF
Sbjct: 127 HAFRTVINGLGNAGLMIVLDNHISQPRWCCSLNDGNGFFGDRNFNPNEWLQGLTYVARHF 186

Query: 189 TWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIISGITFDNDLSFLK 248
           +    VIGMSLRNELRG   N+D W+KYV+ GSHLIH  N  LLV+ISG+ +DNDLS LK
Sbjct: 187 SCQPHVIGMSLRNELRGS-NNVDEWHKYVRRGSHLIHNINPNLLVVISGLNYDNDLSHLK 246

Query: 249 KKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKFEREAGFVVNMED 308
           KK LGY LHNKVVLEAHLY FSG    PESKFVKKPLNI CNQVMEKFEREAGFVV+MED
Sbjct: 247 KKPLGYKLHNKVVLEAHLYSFSGD---PESKFVKKPLNIACNQVMEKFEREAGFVVDMED 306

Query: 309 EPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTYMYRQGQESVDET 368
            PYPLW+SEFGYD+RG NKAQNRFM+CFLA ++ KD+DWA WAFQG+YMYRQGQE  DE+
Sbjct: 307 -PYPLWISEFGYDVRGGNKAQNRFMNCFLARLIGKDIDWALWAFQGSYMYRQGQEDRDES 366

Query: 369 FGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQCIRSNGQGGIVLG 428
           FGVMD+TWT DR+PQL QMLQLAKRINQDPNS ASMSYI+LHPVSGQC++S+G+GGI LG
Sbjct: 367 FGVMDNTWTKDRSPQLKQMLQLAKRINQDPNSKASMSYIMLHPVSGQCVKSDGKGGIELG 426

Query: 429 DCLTPTHWTHSGDGSSMK-LSNGQCLQSAGDGKPLIVSAECSSDGSKWTMASKAKLQLAT 488
           DC TPT W H+GDG+ MK LSNGQCL+SAGDGKP +VSA+CS DGS WT+ASKAKLQLAT
Sbjct: 427 DCTTPTLWNHTGDGTPMKLLSNGQCLKSAGDGKPPMVSADCSGDGSNWTVASKAKLQLAT 486

Query: 489 KSGGENFCLEKKSNTKIVVKRCICLEDELNCFNDPQ 524
           KSGGENFCLEK+S+T IVVK+CICL+DE NCF+DPQ
Sbjct: 487 KSGGENFCLEKESDTSIVVKKCICLKDEWNCFDDPQ 516

BLAST of CsaV3_5G002300 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 1.8e-97
Identity = 199/534 (37.27%), Postives = 301/534 (56.37%), Query Frame = 0

Query: 9   LISLIFFFSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLASHAQSMLAQGLDKRTLK 68
           L++ +    +   S SLPL TRGRWIVD  TG RVKL+CVN   H +  L +GL++  + 
Sbjct: 13  LLTALLLLLVAAPSHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVA 72

Query: 69  DLANDIISRDFNCVRLTWSTHMFTR--YSHMTIIDVLDNLDIQNVKSGVQKHNPKMLNMT 128
            +A+ I S  FNCVRLT+S HM TR  Y++ T+      L++    SG++ +NP++L++ 
Sbjct: 73  TVAHTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLG 132

Query: 129 VDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLEWLQGLDYVAR 188
              A+  VV  L  AG+M++LDNH+S+P+WCC++DDGNGFFGDR+F+   W++GL  +A 
Sbjct: 133 HVAAYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMAT 192

Query: 189 HFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIISGITFDNDLSF 248
           +F     V+ MSLRNELRG  +   +W ++++ G+  +H  N K+LVI+SG+ FD DLSF
Sbjct: 193 YFNNTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSF 252

Query: 249 LKKKSLGYNLHNKVVLEAHLYPFSGSEK--LPESKFVKKPLNIVCNQVMEKFEREAGFVV 308
           L    +      K+V E H Y F    +  LP         N VC     +F+   GFV 
Sbjct: 253 LPVLPVTLPFKEKIVYEGHWYSFGVPWRTGLP---------NDVCKNETGRFKSNVGFVT 312

Query: 309 NMED-EPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTYMYRQGQE 368
           +  +    PL++SEFG D R  N   NR+++C LA++  +DLDWA W   G+Y YR  ++
Sbjct: 313 SSANATAAPLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQ 372

Query: 369 SV---DETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSY-IILHPVSGQCIRS 428
            V   +ET+G  +  W+  RNP     L+  ++  QDP       Y II HP SG C+ S
Sbjct: 373 PVKDFEETYGFFNHDWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLCVES 432

Query: 429 NGQGGIVLGDCLT-PTHWTH--SGDGSSMKLSNGQCLQSAGDGKPLIVSAECSS-DGSKW 488
                + LG C +  + W +  S  G    + +  C+ + G+G P I++  CS+ + + W
Sbjct: 433 GIGNTVHLGSCQSVRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLW 492

Query: 489 TMASKAKLQLATKSGGEN-----FCLEKKSNTKIVVKRCICLEDELNCFNDPQP 525
           +  S A+LQL T+  G++      CL+   +  I    CIC+ D  +C+    P
Sbjct: 493 STVSSAQLQLGTRVLGKDGKEKWMCLDGSKSPLISTNECICITDS-HCYPKLNP 536

BLAST of CsaV3_5G002300 vs. ExPASy TrEMBL
Match: A0A0A0KQT4 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G151620 PE=3 SV=1)

HSP 1 Score: 983.8 bits (2542), Expect = 3.2e-283
Identity = 468/470 (99.57%), Postives = 469/470 (99.79%), Query Frame = 0

Query: 57  MLAQGLDKRTLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIIDVLDNLDIQNVKSGVQ 116
           MLAQGLDKRTLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIIDVLDNLDIQNVKSGVQ
Sbjct: 1   MLAQGLDKRTLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIIDVLDNLDIQNVKSGVQ 60

Query: 117 KHNPKMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLE 176
           KHNPKMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLE
Sbjct: 61  KHNPKMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLE 120

Query: 177 WLQGLDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIIS 236
           WLQGLDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIIS
Sbjct: 121 WLQGLDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIIS 180

Query: 237 GITFDNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKF 296
           GITFDNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKF
Sbjct: 181 GITFDNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKF 240

Query: 297 EREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTY 356
           EREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTY
Sbjct: 241 EREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTY 300

Query: 357 MYRQGQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQC 416
           MYRQGQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQC
Sbjct: 301 MYRQGQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQC 360

Query: 417 IRSNGQGGIVLGDCLTPTHWTHSGDGSSMKLSNGQCLQSAGDGKPLIVSAECSSDGSKWT 476
           IRSNGQGGIVLGDCLTPTHWTHSGDGSSMKLSNGQCLQSAGDGKPLIVSAECSSDGSKWT
Sbjct: 361 IRSNGQGGIVLGDCLTPTHWTHSGDGSSMKLSNGQCLQSAGDGKPLIVSAECSSDGSKWT 420

Query: 477 MASKAKLQLATKSGGENFCLEKKSNTKIVVKRCICLEDELNCFNDPQPHF 527
           MASKAKLQLATKSGGENFCLEKKSNTKIVVKRCICLEDELNCFNDPQP +
Sbjct: 421 MASKAKLQLATKSGGENFCLEKKSNTKIVVKRCICLEDELNCFNDPQPQW 470

BLAST of CsaV3_5G002300 vs. ExPASy TrEMBL
Match: A0A5A7TLU0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G002120 PE=3 SV=1)

HSP 1 Score: 906.0 bits (2340), Expect = 8.5e-260
Identity = 431/471 (91.51%), Postives = 447/471 (94.90%), Query Frame = 0

Query: 57  MLAQGLDKRTLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIIDVLDNLDIQNVKSGVQ 116
           MLAQGLDKR LKDLANDIISRDFNCVRLTWSTHMFTRYSHMTI+DVLD+LDIQNVKSGVQ
Sbjct: 1   MLAQGLDKRPLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIVDVLDDLDIQNVKSGVQ 60

Query: 117 KHNPKMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLE 176
            HNP+MLNMTV HAFKTVV GLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFD LE
Sbjct: 61  THNPEMLNMTVAHAFKTVVDGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDALE 120

Query: 177 WLQGLDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIIS 236
           WLQGLDYVAR+FT HSQVIGMSLRNELRGPYTNMDNWYKYV  GSHLIHTKN  LLVIIS
Sbjct: 121 WLQGLDYVARNFTGHSQVIGMSLRNELRGPYTNMDNWYKYVSNGSHLIHTKNPNLLVIIS 180

Query: 237 GITFDNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKF 296
           G+TFD DLSFLK+K LGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLN+VCNQV EKF
Sbjct: 181 GVTFDTDLSFLKEKPLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNVVCNQVKEKF 240

Query: 297 EREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTY 356
           EREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIV KDLDWAYWAFQGTY
Sbjct: 241 EREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVGKDLDWAYWAFQGTY 300

Query: 357 MYRQGQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQC 416
           MYRQGQESVDE FG+MDSTWT DRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQC
Sbjct: 301 MYRQGQESVDEAFGIMDSTWTKDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQC 360

Query: 417 IRSNGQGGIVLGDCLTPTHWTHSGDGSSMK-LSNGQCLQSAGDGKPLIVSAECSSDGSKW 476
           IRSNGQGGI LGDCLTPT WT+SGDGSSMK LSNGQCL+SAGDGKP +VS ECS + SKW
Sbjct: 361 IRSNGQGGIALGDCLTPTQWTYSGDGSSMKLLSNGQCLKSAGDGKPPVVSTECSGEESKW 420

Query: 477 TMASKAKLQLATKSGGENFCLEKKSNTKIVVKRCICLEDELNCFNDPQPHF 527
           TMASKAKLQLATKSGGE+FCLEKKS+TKIVVKRCICL+DELNCFNDPQP +
Sbjct: 421 TMASKAKLQLATKSGGEDFCLEKKSDTKIVVKRCICLKDELNCFNDPQPQW 471

BLAST of CsaV3_5G002300 vs. ExPASy TrEMBL
Match: A0A1S3AUP6 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103483074 PE=3 SV=1)

HSP 1 Score: 906.0 bits (2340), Expect = 8.5e-260
Identity = 431/471 (91.51%), Postives = 447/471 (94.90%), Query Frame = 0

Query: 57  MLAQGLDKRTLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIIDVLDNLDIQNVKSGVQ 116
           MLAQGLDKR LKDLANDIISRDFNCVRLTWSTHMFTRYSHMTI+DVLD+LDIQNVKSGVQ
Sbjct: 1   MLAQGLDKRPLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIVDVLDDLDIQNVKSGVQ 60

Query: 117 KHNPKMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLE 176
            HNP+MLNMTV HAFKTVV GLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFD LE
Sbjct: 61  THNPEMLNMTVAHAFKTVVDGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDALE 120

Query: 177 WLQGLDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIIS 236
           WLQGLDYVAR+FT HSQVIGMSLRNELRGPYTNMDNWYKYV  GSHLIHTKN  LLVIIS
Sbjct: 121 WLQGLDYVARNFTGHSQVIGMSLRNELRGPYTNMDNWYKYVSNGSHLIHTKNPNLLVIIS 180

Query: 237 GITFDNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKF 296
           G+TFD DLSFLK+K LGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLN+VCNQV EKF
Sbjct: 181 GVTFDTDLSFLKEKPLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNVVCNQVKEKF 240

Query: 297 EREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTY 356
           EREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIV KDLDWAYWAFQGTY
Sbjct: 241 EREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVGKDLDWAYWAFQGTY 300

Query: 357 MYRQGQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQC 416
           MYRQGQESVDE FG+MDSTWT DRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQC
Sbjct: 301 MYRQGQESVDEAFGIMDSTWTKDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQC 360

Query: 417 IRSNGQGGIVLGDCLTPTHWTHSGDGSSMK-LSNGQCLQSAGDGKPLIVSAECSSDGSKW 476
           IRSNGQGGI LGDCLTPT WT+SGDGSSMK LSNGQCL+SAGDGKP +VS ECS + SKW
Sbjct: 361 IRSNGQGGIALGDCLTPTQWTYSGDGSSMKLLSNGQCLKSAGDGKPPVVSTECSGEESKW 420

Query: 477 TMASKAKLQLATKSGGENFCLEKKSNTKIVVKRCICLEDELNCFNDPQPHF 527
           TMASKAKLQLATKSGGE+FCLEKKS+TKIVVKRCICL+DELNCFNDPQP +
Sbjct: 421 TMASKAKLQLATKSGGEDFCLEKKSDTKIVVKRCICLKDELNCFNDPQPQW 471

BLAST of CsaV3_5G002300 vs. ExPASy TrEMBL
Match: A0A0A0KNT6 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G151630 PE=3 SV=1)

HSP 1 Score: 831.2 bits (2146), Expect = 2.7e-237
Identity = 405/523 (77.44%), Postives = 456/523 (87.19%), Query Frame = 0

Query: 1   MAKKIVFYLISLIFFFSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLASHAQSMLAQ 60
           MAK   F LIS I F SLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNL SHAQSM+AQ
Sbjct: 1   MAK--AFPLIS-ILFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQ 60

Query: 61  GLDKRTLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIIDVLDNLDIQNVKSGVQKHNP 120
           GLDKR LKDLAN+I+SRD+NCVRLTWS +MFTRY   TI DVLD LDI  VK+GV+KHNP
Sbjct: 61  GLDKRPLKDLANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVKKHNP 120

Query: 121 KMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLEWLQG 180
           + LNMTV  AFKTVV GLGNAGLM++LDNHISQPRWCCSL DGNGFFGDR+F  +EWL+G
Sbjct: 121 QFLNMTVTSAFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRG 180

Query: 181 LDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIISGITF 240
           L YVARHF+WH +V+GMSLRNELRG   N+  W KYVK GSHLIH  N +LLV+ISG+ +
Sbjct: 181 LAYVARHFSWHPKVVGMSLRNELRGS-NNVGVWRKYVKLGSHLIHRINPRLLVVISGLNY 240

Query: 241 DNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKFEREA 300
           DNDLS+LKKK LGYNL+NKVVLEAHLY FSG    PESKFVKKPLNI CNQVM+KFEREA
Sbjct: 241 DNDLSYLKKKPLGYNLNNKVVLEAHLYSFSGE---PESKFVKKPLNIACNQVMDKFEREA 300

Query: 301 GFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTYMYRQ 360
           GFVV+M+D PYPL+LSEFGYDLRG NKAQNRFMSCFLA I+ KD+DWAYWAFQG+YMYRQ
Sbjct: 301 GFVVDMKD-PYPLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSYMYRQ 360

Query: 361 GQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQCIRSN 420
           GQ+ VDE+FG+MDS+WT DR+P+L QMLQLAKRINQDPNS   MSYI+LHPVSGQC++ +
Sbjct: 361 GQQDVDESFGIMDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQCVKLD 420

Query: 421 GQGGIVLGDCLTPTHWTHSGDGSSMKLSNGQCLQSAGDGKPLIVSAECSSDGSKWTMASK 480
           G+GGI LGDC TPT W H+GDGS MKL NGQCL+SAGDGKP +VSAECS DGS WT+ASK
Sbjct: 421 GKGGIELGDCETPTLWDHTGDGSPMKLWNGQCLKSAGDGKPPVVSAECSGDGSSWTVASK 480

Query: 481 AKLQLATKSGGENFCLEKKSNTKIVVKRCICLEDELNCFNDPQ 524
           AKLQL+TKSGGEN CLEK+S+T IVVK+CICL+DE NCF+DPQ
Sbjct: 481 AKLQLSTKSGGENICLEKESDTSIVVKKCICLKDEWNCFDDPQ 515

BLAST of CsaV3_5G002300 vs. ExPASy TrEMBL
Match: A0A5A7TIV3 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G002130 PE=3 SV=1)

HSP 1 Score: 807.7 bits (2085), Expect = 3.2e-230
Identity = 396/526 (75.29%), Postives = 453/526 (86.12%), Query Frame = 0

Query: 1   MAKKIVFYLISLIFFFSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLASHAQSMLAQ 60
           MAK   F LISL+ F SLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNL SHAQSM+AQ
Sbjct: 1   MAK--AFSLISLL-FLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQ 60

Query: 61  GLDKRTLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIIDVLDNLDIQNVKSGVQKHNP 120
           GLDKR LKDLAN+I+SRD+NCVRLTWS +MFTR+   TI DVLD LDI  VK+GV+KHNP
Sbjct: 61  GLDKRPLKDLANEIVSRDYNCVRLTWSVYMFTRHPFETIGDVLDGLDINKVKNGVKKHNP 120

Query: 121 KMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLEWLQG 180
           ++LNMTV  AFKTVV GLGNAGLM+++DNHISQPRWCCSL DGNGFFGDR+F  +EWL+G
Sbjct: 121 QILNMTVTSAFKTVVDGLGNAGLMVIMDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRG 180

Query: 181 LDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIISGITF 240
           L +          V+GMSLRNELRG   N+D W KYVK+GSHLIH  N +LLV+ISG+ +
Sbjct: 181 LAF----------VVGMSLRNELRGS-NNVDVWRKYVKQGSHLIHRINPRLLVVISGLNY 240

Query: 241 DNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKFEREA 300
           DNDLS+LKKK LGYNLHNKVVLEAHLY FSG    PESKFVKKPLNI CNQVM+KFEREA
Sbjct: 241 DNDLSYLKKKPLGYNLHNKVVLEAHLYSFSGE---PESKFVKKPLNIACNQVMDKFEREA 300

Query: 301 GFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTYMYRQ 360
           GFVV+M++ PYPL+LSEFGYDLRG NKAQNRFMSCFLA I+ KD++WAYWAFQG+YMYRQ
Sbjct: 301 GFVVDMKN-PYPLFLSEFGYDLRGVNKAQNRFMSCFLARIIGKDIEWAYWAFQGSYMYRQ 360

Query: 361 GQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQCIRSN 420
           GQ+ VDE+FG+MDSTWT DR+P+L QML+LAKRINQDPNS A +SYI+LHPVSGQC++ +
Sbjct: 361 GQQDVDESFGIMDSTWTKDRSPRLQQMLKLAKRINQDPNSKAPLSYIMLHPVSGQCVQLD 420

Query: 421 GQGGIVLGDCLTPTHWTHSGDGSSMKLSNGQCLQSAGDGKPLIVSAECSSDGSKWTMASK 480
           G+GGI LGDC  PT W H+GDGS MKLSNGQCL+SAGDGKP +VSAECS DGS WT+ASK
Sbjct: 421 GKGGIELGDCEKPTLWDHTGDGSPMKLSNGQCLKSAGDGKPPVVSAECSGDGSSWTVASK 480

Query: 481 AKLQLATKSGGENFCLEKKSNTKIVVKRCICLEDELNCFNDPQPHF 527
           AKLQLATKSGGE  CLEK+S+T IVVK+CICL+DE NCF+DPQP +
Sbjct: 481 AKLQLATKSGGEKICLEKESDTSIVVKKCICLKDEWNCFDDPQPQW 508

BLAST of CsaV3_5G002300 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 370.5 bits (950), Expect = 2.5e-102
Identity = 184/497 (37.02%), Postives = 285/497 (57.34%), Query Frame = 0

Query: 26  PLSTRGRWIVDSKTGQRVKLSCVNLASHAQSMLAQGLDKRTLKDLANDIISRDFNCVRLT 85
           PLST  RWI+D K GQRVKL+CVN  SH Q ++A+GL K+++ DLA  I++  FNCVR T
Sbjct: 4   PLSTNSRWIIDEK-GQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFT 63

Query: 86  WSTHMFTR---YSHMTIIDVLDNLDIQNVKSGVQKHNPKMLNMTVDHAFKTVVYGLGNAG 145
           W   + T     +++T+     +L + +  SG +  NP M+++ +  A+K VV  LGN  
Sbjct: 64  WPLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNN 123

Query: 146 LMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLEWLQGLDYVARHFTWHSQVIGMSLRNE 205
           +M++LDNH+++P WCC  +DGNGFFGD  FD   W+ GL  +A  F   + V+GMSLRNE
Sbjct: 124 VMVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNE 183

Query: 206 LRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIISGITFDNDLSFLKKKSLGYNLHNKVVL 265
           LRGP  N+D+W+KY+++G+  +H  N  +LVI+SG+++D DLSF++ + +      K+V 
Sbjct: 184 LRGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNLTFTRKLVF 243

Query: 266 EAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKFEREAGFVVNMEDEPYPLWLSEFGYDL 325
           E H Y F+ +     + +  K  N  C ++++  E   GF  N+ D  +P++LSEFG DL
Sbjct: 244 ELHRYSFTNT-----NTWSSKNPNEACGEILKSIENGGGF--NLRD--FPVFLSEFGIDL 303

Query: 326 RGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTYMYRQGQESVDETFGVMDSTWTNDRNP 385
           RG N   NR++ C L      D+DW+ W  QG+Y  R+G   + E +G++DS W   R+ 
Sbjct: 304 RGKNVNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQ 363

Query: 386 QLNQMLQLAKRINQDPNSNASMSYIILHPVSGQCIRSN--GQGGIVLGDCLTPTHWTHSG 445
              Q L L     Q P S + +  ++ HP++G C+  +      + LG C     W+++ 
Sbjct: 364 SFLQRLSLILSPLQGPGSQSKVYNLVFHPLTGLCMLQSILDPTKVTLGLCNESQPWSYTP 423

Query: 446 DGSSMKLSNGQCLQSAGDGKPLIVS-AECSSDG-SKWTMASKAKLQLATKSGGENFCLEK 505
             +        CL+S G   P+ +S   CSS   S+W   S + + LA KS   + CL+ 
Sbjct: 424 QNTLTLKDKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLAAKSTNNSLCLDV 483

Query: 506 KSNTKIVVKRCICLEDE 516
                ++   C C++ E
Sbjct: 484 DETNNLMASNCKCVKGE 490

BLAST of CsaV3_5G002300 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 365.9 bits (938), Expect = 6.1e-101
Identity = 195/527 (37.00%), Postives = 300/527 (56.93%), Query Frame = 0

Query: 3   KKIVFYLISLIFFFSLL----VLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLASHAQSML 62
           K + F      FFFS +    V + S PLST  RWIVD + G RVKL C N  SH Q ++
Sbjct: 7   KHMFFIFPFFCFFFSFIAQNTVPNMSYPLSTSSRWIVD-ENGLRVKLVCANWPSHLQPVV 66

Query: 63  AQGLDKRTLKDLANDIISRDFNCVRLTWSTHMFTR---YSHMTIIDVLDNLDIQNVKSGV 122
           A+GL K+ +  +A  I+   FNCVRLTW   + T     +++T+     +L + +   G 
Sbjct: 67  AEGLSKQPVDAVAKKIVEMGFNCVRLTWPLDLMTNETLANNVTVRQSFQSLGLNDDIVGF 126

Query: 123 QKHNPKMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTL 182
           Q +NP ++++ +  A+KTVV  LGN  +M++LDNH+++P WCC+ DDGNGFFGD+ FD  
Sbjct: 127 QTNNPSIIDLPLIEAYKTVVTTLGNNDVMVILDNHLTKPGWCCANDDGNGFFGDQFFDPT 186

Query: 183 EWLQGLDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVII 242
            W+  L  +A  F   S V+GMSLRNELRGP  N+++W+KY+++G+  +H+ N K+LVI+
Sbjct: 187 VWVAALKKMAATFNGVSNVVGMSLRNELRGPKQNVNDWFKYMQQGAEAVHSANNKVLVIL 246

Query: 243 SGITFDNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEK 302
           SG++FD DLSF++ + +  +   K+V E H Y FS       + +     N +C +V+ +
Sbjct: 247 SGLSFDADLSFVRSRPVKLSFTGKLVFELHWYSFSDG-----NSWAANNPNDICGRVLNR 306

Query: 303 FEREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGT 362
                G+++N   + +PL+LSEFG D RG N   NR+  C        D+DW+ WA  G+
Sbjct: 307 IGNGGGYLLN---QGFPLFLSEFGIDERGVNTNDNRYFGCLTGWAAENDVDWSLWALTGS 366

Query: 363 YMYRQGQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQ 422
           Y  RQG+  ++E +GV+DS W + RN    Q +   +   Q P        ++ HP++G 
Sbjct: 367 YYLRQGKVGMNEYYGVLDSDWISVRNSSFLQKISFLQSPLQGPGPRTDAYNLVFHPLTGL 426

Query: 423 CI-RS-NGQGGIVLGDCLTPTHWTHSGDGSSMKLSNGQCLQSAGDGKPLIVS-AECSSDG 482
           CI RS +    + LG C +   W+++     +K     CLQS G   P+ ++   CS+ G
Sbjct: 427 CIVRSLDDPKMLTLGPCNSSEPWSYTKKALRIK-DQQLCLQSNGPKNPVTMTRTSCSTSG 486

Query: 483 SKWTMASKAKLQLA-TKSGGENFCLEKKSNTKIVVKRCICLEDELNC 519
           SKW   S +++ LA T S   + CL+  +   +V   C CL  + +C
Sbjct: 487 SKWQTISASRMHLASTTSNKTSLCLDVDTANNVVANACKCLSKDKSC 523

BLAST of CsaV3_5G002300 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 343.6 bits (880), Expect = 3.2e-94
Identity = 203/530 (38.30%), Postives = 297/530 (56.04%), Query Frame = 0

Query: 7   FYLISLIFFFSLLVLSQSLPLSTRGRWIV-DSKTGQRVKLSCVNLASHAQSMLAQGLDKR 66
           F+ IS +F    ++ + + P ST  RWIV D   G+RVKL+CVN  SH ++ +A+GL K+
Sbjct: 4   FFFIS-VFLLPYVITTFAFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLSKQ 63

Query: 67  TLKDLANDIISRDFNCVRLTWSTHMFTRYS---HMTIIDVLDNLDIQNVKSGVQKHNPKM 126
            L  +A  I+S  FNCVRLTW  ++ T  S    MT+   L    +    SG Q HNP +
Sbjct: 64  PLDAIAEKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNPTI 123

Query: 127 LNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLEWLQGLD 186
           L++ +  AF+ VVY L    +M++LDNHISQP WCCS +DGNGFFGD+H +   W++GL 
Sbjct: 124 LDLPLIKAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKGLK 183

Query: 187 YVARHF-TWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIISGITFD 246
            +A  F    S V+GMSLRNELRGP  N+ +WYKY++EG+  +H+ N  +LVI+SG+ + 
Sbjct: 184 KMASMFANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLNYA 243

Query: 247 NDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKFEREAG 306
            DLSFL+++    +   KVV E H Y F  +       +    LN +C +  EK  + +G
Sbjct: 244 TDLSFLRERPFEVSFRRKVVFEIHWYGFWNT-------WEGDNLNKICGKETEKMMKMSG 303

Query: 307 FVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTYMYRQG 366
           F++   ++  PL++SEFG D RG+N   N+F+SCF+A    +DLDW+ W   G+Y  R+ 
Sbjct: 304 FLL---EKGIPLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYIREK 363

Query: 367 QESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDP---NSNASMSYIILHPVSGQCIR 426
               DE++GV+D  W++ RN  + QM+       Q P           I+ HP +G CI 
Sbjct: 364 SIGSDESYGVLDFNWSSIRNSTILQMISAI----QTPFIGLMETQPKKIMFHPSTGLCIV 423

Query: 427 SNGQGGIVLGDCLTPTHWTHSGDGSSMKLSNGQ--CLQSAGDGKPLIVSAECS-SDGSKW 486
                 + LG C     W  S     + L+  Q  CL++   GK + +    S S  SKW
Sbjct: 424 RKSLFQLKLGSCNRSESWRLSSH-RVLSLAEEQILCLKAYEKGKSVKLRLFFSESYCSKW 483

Query: 487 TMASKAKLQLAT-KSGGENFCLE-KKSNTKIVVKRCICLEDELNCFNDPQ 524
            + S +K+QL++    G + CL+    N  IV   C CL    +C  DP+
Sbjct: 484 KLFSDSKMQLSSITKNGFSVCLDVDTENNNIVTNSCKCLRGNSSC--DPR 515

BLAST of CsaV3_5G002300 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 328.9 bits (842), Expect = 8.3e-90
Identity = 189/529 (35.73%), Postives = 283/529 (53.50%), Query Frame = 0

Query: 1   MAKKIVFYLISLIFFFSL--LVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLASHAQSML 60
           MAK I    + L  F SL  L L+   PL T+ RWIV++K G RVKL+C N  SH + ++
Sbjct: 1   MAKAIAL-TVFLFLFLSLISLTLATDYPLFTKSRWIVNNK-GHRVKLACANWPSHLKPVV 60

Query: 61  AQGLDKRTLKDLANDIISRDFNCVRLTWSTHMF---TRYSHMTIIDVLDNLDIQNVKSGV 120
           A+GL  + +  ++  I    FNCVRLTW   +    T   ++T+    +   + +   G+
Sbjct: 61  AEGLSSQPMDSISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGI 120

Query: 121 QKHNPKMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTL 180
             HNP ++N  + + F+ VVY LG   +M++LDNH + P WCCS DD + FFGD  F+  
Sbjct: 121 YTHNPYIVNTPLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPD 180

Query: 181 EWLQGLDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVII 240
            W+ GL  +A  F     V+GMSLRNELRG      +WYKY+++G+  +HT N  +LVI+
Sbjct: 181 LWMLGLKKMATIFMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVIL 240

Query: 241 SGITFDNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEK 300
           SG+ FD DLSFLK + +  +   K+VLE H Y F+        ++    +N  C+Q+  K
Sbjct: 241 SGLNFDADLSFLKDRPVNLSFKKKLVLELHWYSFTDG----TGQWKSHNVNDFCSQMFSK 300

Query: 301 FEREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGT 360
             R  GFV+   D+ +PL+LSEFG D RG +   NR+M+C LA    KDLDWA WA  G 
Sbjct: 301 ERRTGGFVL---DQGFPLFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGV 360

Query: 361 YMYRQGQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQ 420
           Y +R+G+  V E +G++D+ W N  N    + L + +  +  P    +    I HP++G 
Sbjct: 361 YYFREGKRGVVEAYGMLDANWHNVHNYTYLRRLSVIQPPHTGPGVKHNHHKKIFHPLTGL 420

Query: 421 CIRSNG---QGGIVLGDCLTPTHWTHSGDG-SSMKLSNGQCLQ-SAGDGKPLIVSAECSS 480
           C+       +  + LG C     W++S  G   ++  +  CL+     GK + +   C  
Sbjct: 421 CLVRKSHCHESELTLGPCTKDEPWSYSHGGILEIRRGHKSCLEGETAVGKSVKLGRIC-- 480

Query: 481 DGSKWTMASKAKLQLA-TKSGGENFCLEKKSNTKIVVKRCICLEDELNC 519
             +K    S  K+ L+   S G   CL+  S+  +V   C CL  +  C
Sbjct: 481 --TKIEQISATKMHLSFNTSDGSLVCLDVDSDNNVVANSCNCLTGDTTC 516

BLAST of CsaV3_5G002300 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 302.4 bits (773), Expect = 8.3e-82
Identity = 191/529 (36.11%), Postives = 274/529 (51.80%), Query Frame = 0

Query: 1   MAKKIVFYLISLIFFFSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLASHAQSMLAQ 60
           MA+   F L   +F  S   L+ S PLST+ RWIVD K GQRVKL+CVN  +H Q  +A+
Sbjct: 1   MARSFYFCLF-FLFISSTSKLTTSYPLSTKSRWIVDEK-GQRVKLACVNWPAHLQPTVAE 60

Query: 61  GLDKRTLKDLANDIISRDFNCVRLTWSTHMFTRYS---HMTIIDVLDNLDIQNVKSGVQK 120
           GL K+ L  ++  I+S  FNCVRLTW   + T  +    +T+    ++L +     G+Q 
Sbjct: 61  GLSKQPLDSISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQT 120

Query: 121 HNPKMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLEW 180
           HNPK+L++ + +AF+ VV  LG  G+M++LDNH++ P WCC  +D + FFG  HFD L W
Sbjct: 121 HNPKLLHLPLFNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVW 180

Query: 181 LQGLDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIISG 240
            +GL  +A  F   + VIGMSLRNE RG     D W++++ +G+  +H  N KLLVI+SG
Sbjct: 181 AKGLRKMATLFRNFTHVIGMSLRNEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILSG 240

Query: 241 ITFDNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKFE 300
           I FD +LSFL+ +S+  +  +K+V E H Y FS         + K   N  C +++EK  
Sbjct: 241 IDFDTNLSFLRDRSVNVSFTDKLVFELHWYSFSDG----RDSWRKHNSNDFCVKIIEKVT 300

Query: 301 REAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTYM 360
              GF++      +PL LSEFG D RG + + NR+M+C +A     DLDWA WA  G Y 
Sbjct: 301 HNGGFLLG---RGFPLILSEFGTDQRGGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYY 360

Query: 361 YRQGQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQCI 420
            R G                    P L             PN N     ++ HP +G C+
Sbjct: 361 LRTG--------------------PGLR------------PNKN-----LLFHPSTGLCV 420

Query: 421 RSNGQGGIV---LGDCLTPTHWTHSGDGSSMKLSNGQCLQS---AGDGKPLIVSAECSSD 480
            +N    I    LG C     WT +     + + N  C+++    G    L V  +CS  
Sbjct: 421 TNNPSDNIPTLRLGPCPKSDPWTFNPSEGILWI-NKMCVEAPNVVGQKVKLGVGTKCSKL 478

Query: 481 GSKWTMASKAKLQLATK-SGGENFCLE-KKSNTKIVVKRCICLEDELNC 519
           G      S  K+ L+ K S G   CL+  + +  +V  RC  L  + +C
Sbjct: 481 G----QISATKMHLSFKTSNGLLLCLDVDERDNSVVANRCKFLTMDASC 478

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAE8647841.10.0e+00100.00hypothetical protein Csa_000559 [Cucumis sativus][more]
XP_004143962.16.6e-28399.57glycosyl hydrolase 5 family protein [Cucumis sativus][more]
XP_008437728.11.8e-25991.51PREDICTED: uncharacterized protein LOC103483074 [Cucumis melo] >KAA0042707.1 maj... [more]
XP_038907078.11.2e-24780.11glycosyl hydrolase 5 family protein-like [Benincasa hispida][more]
XP_038907081.15.3e-24079.26glycosyl hydrolase 5 family protein-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
C0HLA01.8e-9737.27Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KQT43.2e-28399.57Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G151620 PE=3... [more]
A0A5A7TLU08.5e-26091.51Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3AUP68.5e-26091.51Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103483074 PE=3 SV... [more]
A0A0A0KNT62.7e-23777.44Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G151630 PE=3... [more]
A0A5A7TIV33.2e-23075.29Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
AT3G26140.12.5e-10237.02Cellulase (glycosyl hydrolase family 5) protein [more]
AT1G13130.16.1e-10137.00Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26130.13.2e-9438.30Cellulase (glycosyl hydrolase family 5) protein [more]
AT5G17500.18.3e-9035.73Glycosyl hydrolase superfamily protein [more]
AT5G16700.18.3e-8236.11Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.80.10.50coord: 403..513
e-value: 3.4E-7
score: 32.4
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 24..397
e-value: 9.0E-58
score: 198.1
NoneNo IPR availablePANTHERPTHR31263CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)coord: 8..524
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 63..352
e-value: 1.2E-14
score: 54.4
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 402..509
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 25..392

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_5G002300.1CsaV3_5G002300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds