CsaV3_4G030360 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_4G030360
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionProteinase inhibitor I25, cystatin
Locationchr4: 20188795 .. 20192341 (-)
RNA-Seq ExpressionCsaV3_4G030360
SyntenyCsaV3_4G030360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAGGCATCAATAGTTTGACTAAGATGGTAGAATAATCTTTTGTCTCCAAACAAATGAATTGATTCGAAACTATCCCCTAATCCATCCATTGGGAGTTTACATAGATGTGGATTAGATTCTATGTTATCAATGATGTGATGTTGAAAACAACAGTTATATAGAAATACCAAACTTGATCTCAATATTAATATCATATTGAGATCAATGAAGGGTTAGAAAATGTTTAGCCACCAAAGGTATTGTATAATGAGTGTTGAAGAAGCATATCACACTTCGAAACCTATCGAGGATATAAATGATGATCTGCAAGTGCAGTCAATCATAGGACAGTTGGCTGTTGAAGACCATAACAAGAAAACAGGAGACAATTTGGAGCTTGTGGATGTGGTGAATGGTTTGAGAAGTGGAATTTTTGTTCAACCTGGGAGTACTCATGAAGGAATATTATATCATCTTTTGGTGGAGGCTAAAACCATAGAAGGAATTAATTGGACATATGTGGCCAAGTTGTTAGAGCTTTACGTAGGCTGCCGAATAAGGTATGAATTCAAATCTTTTGAACCTCTTCTCCCATACTACAACCCCTAATCTAATATTTTACCCTCATCTCTCTACCCTACTTCTCACTCACATTCTAATTAAATAAATTGTATCTTTGTATATCAAAAATGTTCCTACCATATATATAAAAAAAAAGTAGTATTGAATATATTTATATTTTTAGTTTTGGGTAAATTACAAAAACCATATTTAAACATTTGAATTCAGCTTGTAATAATGATACGATTTATACACTTATCTAAGTTTGAGAATCCAATTTTAAAATTTGAATATATTTGAAAGTTTAATGTATTGTGCATATATGTATGTACGTGTCATTCCTAACTATTATTTGTGCGTATTCATGTGTGTGATCTCCGATTATCATTGTGCCTATAAGCACCGACAAATTTAGTATAGGCCTAGGAGGATCGAGTTCTCCCTCAACTTTATTATCTTTATAATATATGTATAAACAAAACTAATTAATGTTTAATATTTATTGATAGTTTAGTGGTAACTATGCTTGTCAATCTCTCTTTCGATCAGAGATCAAGTCCCTATAAGTGATGTGTATCAAAACCGAAAACACCTTAATTCCCACAACTCATAAGAACATTATAATCTCTTTTTTATTTTTTATTTTTTTCAATTTAACTCAAATTAATTCAATTTATTTTTATCATTTTTTTGGGTTCATTTGTATCAACCAATATCTACTGCTTTCTATCCTAAAACTAAATGATGACAAATAGTTAATCCAAGAAGGAAAATTGAGGAAGTTTGATATTAATAAAATTAGAGGCACTTGCATTCATCCAAAAATATAAAATTAAAATTAAGCTCATGATCCAACATTTTAAAATTTGGAAGAATGACAAATTAATTGCTACAATCCAATTGGTTTGATTATCAATAATATCGATTTGTCTGTTCTTTGACTACTGTCCGATTGTTATTTGATGTTCATTGTTATATGATTGTTAATGATACCGATTTATCATCTCCTTGTTATTTAATTTAATATTAATTTTTATTTAATTACAAATGATACTAATTGCTATATATGATTTTACTTTTTTTTTTATATAATTACTATGTAGGATTATATGATATAATCATCTCATCACCTTCCAGTTTTTTTTTTTTTTTTTTTGTTAATTATTAAGTAGTTTCTATCACACCCTCTCCTGAAGCCTTTCTCTAACTCCTAACCTTGGCCCCAAAAGAGGGTGTGAGGGTTGACAAGGATTGCCATAGTTTGACAACACTTACTAACCATTTAACCTAACCGACCCAACCAAAAGTCACAAAATAACTTAAGAAAACAATATAAGCTATATGTTTTGCAAATAACTCGAACTACTAGCTACAACCTTAAAGTGTTATTAAAGCATTTTAGTGTAAATTTTGCTTTGATGTTAGTTTTAAACTGACATTATAGATGCCCTACAATGTTAATGTCTTCTGCATCGGTTTGTCACAAATTTTATTCAATAATTACCATATTGTCCAATCCATTTTTAACGACGATCAAATACTTAAAAATCGACATCAAATGTATCGGTATATATTGTTTACGCATGTGCAAATAAATAATCTTTTTTTTTTCAAGAAGCAATAATATCTCTTCTATTGTATACAACATAACTCTCAACAACACAAAATTTATCTCATTCACAACAAAGAAAGTGACAAATCGCTTAACTAAGTAACCTATTAGCAAATTACATTGATCAATATATACCCAAAGCTTTCAAAGTATAACGAACGAGGGAGTTGTCTAATATGAAGGATCATAGAGTTGTTGTCCCAAAAATATCTCAGTAAGGAAGATATGCTCACTTTAAGTACATAAAACAAATAATTAAAAATAAATTATTAATACCAAAAGAAATTTTTTTTTAAAAAAAGTACAAAACTTATATTTAATTTATACAAACTATTAAACCATGGAAGACTTAAAATCATATAACTTTAGACAACCAGCATATATTAAATTCCTAGAATTAAAACAATCTAACTTTCAAAAGTTATAAGGAGAATTATGGAGGGAGATATCATTTACAAAATAATATCTAAAATTAAGTACCCAATAATAAATCAAATATCAATTAAAAATGTTTCCATTATTTATCTTCATTTCTTTTTCTTTCAAATACCCAGTTGTCGCCCCCTCTTCACAGTGCTCTGTCCGGCCACAACTTCCATCATCGAACCCACCTCCATTCGACAAAGGTAGCCGCGAGCCAAAGCTTCCTCTTCGCTCCATCATGTCTTGATTCGTGAAGTCGAGTTGAGCCGCTTCGTCATCGAGCTCCCTGCGCAGGATTTTCAAAGTCAAACCAAGTGTCAGCCTTGAGTTGTCGTCCTTGACGCTTTTCGTTGCTCGTTGATGCTCTCTGTAGCTGCTACTCAAGTTTTGGTTAAGATGGTGATTTTGGTAGGTGTTCTTTTTTAGGCTTTATTGGGGATTTCTAATAGGAAACTTGCGGTGGGTAGCATTTTGAAAATATGACAATATAGCAAATATTGTTTTAGAATTTTGCAAATACGACAAAATTCTCACGTCTGAAAATATTTTTCATCTAAAATTTGTTATTATTTCCAAATTACCATTCAATAACTTATTTCTTCTTCTCTTTTTAATGCCTTAATCATCCCTTATTTCTTCTTCCATTTTTTAAGGCTTTGATCATCTTTTTTTTATACGGGATAAAAATAATTTGTGCTTTCTGTCTTAATCTTGATTTTAGAATATCAATGGTACATTAATTGCAATATGAAATATAATAGTTATTGATCAGAGTAATCTAACTTCGTTTTTGAATTTTCTCTACTTTTCTTGATGTGTATTTTGAGTTAAATCTGATTTTAAATGATTTTGTTTTGTTTTTCTACATTGGTTTTGTTTTGGTTTTACTTAATTTGTATAAGTCGTTTTGTATATTAGTGTTCTGATGAACTTATATTATGTATTAGTTGGTTGATATACTTACTACGTG

mRNA sequence

ATGTTTAGCCACCAAAGGTATTGTATAATGAGTGTTGAAGAAGCATATCACACTTCGAAACCTATCGAGGATATAAATGATGATCTGCAAGTGCAGTCAATCATAGGACAGTTGGCTGTTGAAGACCATAACAAGAAAACAGGAGACAATTTGGAGCTTGTGGATGTGGTGAATGGTTTGAGAAGTGGAATTTTTGTTCAACCTGGGAGTACTCATGAAGGAATATTATATCATCTTTTGGTGGAGGCTAAAACCATAGAAGGAATTAATTGGACATATGTGGCCAAGTTGTTAGAGCTTTACGTAGGCTGCCGAATAAGTTGTCGCCCCCTCTTCACAGTGCTCTGTCCGGCCACAACTTCCATCATCGAACCCACCTCCATTCGACAAAGGTAG

Coding sequence (CDS)

ATGTTTAGCCACCAAAGGTATTGTATAATGAGTGTTGAAGAAGCATATCACACTTCGAAACCTATCGAGGATATAAATGATGATCTGCAAGTGCAGTCAATCATAGGACAGTTGGCTGTTGAAGACCATAACAAGAAAACAGGAGACAATTTGGAGCTTGTGGATGTGGTGAATGGTTTGAGAAGTGGAATTTTTGTTCAACCTGGGAGTACTCATGAAGGAATATTATATCATCTTTTGGTGGAGGCTAAAACCATAGAAGGAATTAATTGGACATATGTGGCCAAGTTGTTAGAGCTTTACGTAGGCTGCCGAATAAGTTGTCGCCCCCTCTTCACAGTGCTCTGTCCGGCCACAACTTCCATCATCGAACCCACCTCCATTCGACAAAGGTAG

Protein sequence

MFSHQRYCIMSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPGSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRISCRPLFTVLCPATTSIIEPTSIRQR*
Homology
[Close]
BLAST of CsaV3_4G030360 vs. NCBI nr
Match: XP_031739698.1 (uncharacterized protein LOC116403239 [Cucumis sativus] >KAE8649718.1 hypothetical protein Csa_012480 [Cucumis sativus])

HSP 1 Score: 269.2 bits (687), Expect = 1.8e-68
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0 

Query: 1   MFSHQRYCIMSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGL 60
           MFSHQRYCIMSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGL
Sbjct: 1   MFSHQRYCIMSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGL 60

Query: 61  RSGIFVQPGSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRISCRPLFTVLCPATT 120
           RSGIFVQPGSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRISCRPLFTVLCPATT
Sbjct: 61  RSGIFVQPGSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRISCRPLFTVLCPATT 120

Query: 121 SIIEPTSIRQR 132
           SIIEPTSIRQR
Sbjct: 121 SIIEPTSIRQR 131

[Close]
BLAST of CsaV3_4G030360 vs. NCBI nr
Match: TYK05428.1 (Proteinase inhibitor I25, cystatin [Cucumis melo var. makuwa])

HSP 1 Score: 188.3 bits (477), Expect = 4.1e-44
Identity = 91/98 (92.86%), Postives = 95/98 (96.94%), Query Frame = 0 

Query: 10  MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPG 69
           MSVEEAYHTSKPIE+INDDLQVQSIIG+LAVEDHNKKTG+NLE VDVVNGLRS +FVQPG
Sbjct: 1   MSVEEAYHTSKPIENINDDLQVQSIIGKLAVEDHNKKTGENLEFVDVVNGLRSEVFVQPG 60

Query: 70  STHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIS 108
            THEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIS
Sbjct: 61  CTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIS 98

[Close]
BLAST of CsaV3_4G030360 vs. NCBI nr
Match: KGN54823.1 (hypothetical protein Csa_012607 [Cucumis sativus])

HSP 1 Score: 89.0 bits (219), Expect = 3.4e-14
Identity = 52/95 (54.74%), Postives = 69/95 (72.63%), Query Frame = 0 

Query: 10  MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVED-HNKKTGDNLELVDVVNGLRSGIFVQP 69
           MS+EE+ H  KPI DIND   VQ+ +G+LAVE+ ++K +G+ L+ V VVNGL+S  ++ P
Sbjct: 1   MSIEESRHRLKPIIDINDP-HVQA-LGELAVENLYSKNSGEKLKFVRVVNGLKSDKYIGP 60

Query: 70  GSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVG 104
           G T E ILYHL++EAKT E INWTY  KL E+  G
Sbjct: 61  GFT-EVILYHLVLEAKTNEEINWTYATKLEEVSGG 92

[Close]
BLAST of CsaV3_4G030360 vs. NCBI nr
Match: XP_008442277.1 (PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa] >TYK05422.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa])

HSP 1 Score: 88.2 bits (217), Expect = 5.8e-14
Identity = 48/88 (54.55%), Postives = 62/88 (70.45%), Query Frame = 0 

Query: 10 MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPG 69
          MS+  A+   KPIEDIND+L+VQ  +G L V+DHNKKTG+NL+ V VVNGL +  F  P 
Sbjct: 1  MSIMSAF---KPIEDINDNLEVQD-LGNLTVDDHNKKTGENLKFVRVVNGLAAS-FSDPI 60

Query: 70 STHEGILYHLLVEAKTIEGINWTYVAKL 98
          S   G+LY L++EA T   INWTY A++
Sbjct: 61 SPLGGVLYELVLEAITTSKINWTYKARV 83

[Close]
BLAST of CsaV3_4G030360 vs. NCBI nr
Match: KAE8649716.1 (hypothetical protein Csa_012476 [Cucumis sativus])

HSP 1 Score: 77.8 bits (190), Expect = 7.8e-11
Identity = 49/95 (51.58%), Postives = 59/95 (62.11%), Query Frame = 0 

Query: 20  KPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPGSTHEGILYHL 79
           +PI+DIND  QVQ  IG L V+DHNKKTG+NL+ V VVNGL +G FV P S   G +Y L
Sbjct: 8   EPIKDINDP-QVQE-IGNLTVDDHNKKTGENLKFVRVVNGLFAG-FVDPFSPLGGTVYEL 67

Query: 80  LVEAKTIEGINWTYVAKLLELYVGCRISCRPLFTV 115
           ++EA     INWTY  K     V    + RPL  V
Sbjct: 68  VLEAINAGKINWTYKVK-----VSASTTIRPLGVV 94

[Close]
BLAST of CsaV3_4G030360 vs. ExPASy TrEMBL
Match: A0A0A0L3W1 (Proteinase inhibitor I25, cystatin OS=Cucumis sativus OX=3659 GN=Csa_4G508490 PE=4 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 1.4e-53
Identity = 106/106 (100.00%), Postives = 106/106 (100.00%), Query Frame = 0 

Query: 1   MFSHQRYCIMSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGL 60
           MFSHQRYCIMSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGL
Sbjct: 1   MFSHQRYCIMSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGL 60

Query: 61  RSGIFVQPGSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRI 107
           RSGIFVQPGSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRI
Sbjct: 61  RSGIFVQPGSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRI 106

[Close]
BLAST of CsaV3_4G030360 vs. ExPASy TrEMBL
Match: A0A5D3C2L1 (Proteinase inhibitor I25, cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001230 PE=4 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 2.0e-44
Identity = 91/98 (92.86%), Postives = 95/98 (96.94%), Query Frame = 0 

Query: 10  MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPG 69
           MSVEEAYHTSKPIE+INDDLQVQSIIG+LAVEDHNKKTG+NLE VDVVNGLRS +FVQPG
Sbjct: 1   MSVEEAYHTSKPIENINDDLQVQSIIGKLAVEDHNKKTGENLEFVDVVNGLRSEVFVQPG 60

Query: 70  STHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIS 108
            THEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIS
Sbjct: 61  CTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIS 98

[Close]
BLAST of CsaV3_4G030360 vs. ExPASy TrEMBL
Match: A0A0A0L191 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 1.2e-17
Identity = 57/98 (58.16%), Postives = 70/98 (71.43%), Query Frame = 0 

Query: 10  MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNK-KTGDNLELVDVVNGLRSGIFVQP 69
           MS+EE +H  KPI DIND   VQ IIG+LAVEDH+    G+ L+ V VVNGL+S  ++ P
Sbjct: 1   MSIEEIHHRLKPIIDINDP-HVQ-IIGKLAVEDHDSHNPGEKLKFVRVVNGLKSDKYIGP 60

Query: 70  GSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRI 107
           G T E ILYHL++EAKT E INWTY  KL ++Y GC I
Sbjct: 61  GFT-EVILYHLVLEAKTNEEINWTYATKLEKVYSGCLI 95

[Close]
BLAST of CsaV3_4G030360 vs. ExPASy TrEMBL
Match: A0A0A0KZ98 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508510 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.6e-14
Identity = 52/95 (54.74%), Postives = 69/95 (72.63%), Query Frame = 0 

Query: 10  MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVED-HNKKTGDNLELVDVVNGLRSGIFVQP 69
           MS+EE+ H  KPI DIND   VQ+ +G+LAVE+ ++K +G+ L+ V VVNGL+S  ++ P
Sbjct: 1   MSIEESRHRLKPIIDINDP-HVQA-LGELAVENLYSKNSGEKLKFVRVVNGLKSDKYIGP 60

Query: 70  GSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVG 104
           G T E ILYHL++EAKT E INWTY  KL E+  G
Sbjct: 61  GFT-EVILYHLVLEAKTNEEINWTYATKLEEVSGG 92

[Close]
BLAST of CsaV3_4G030360 vs. ExPASy TrEMBL
Match: A0A5A7TJK0 (Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001140 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 2.8e-14
Identity = 48/88 (54.55%), Postives = 62/88 (70.45%), Query Frame = 0 

Query: 10 MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPG 69
          MS+  A+   KPIEDIND+L+VQ  +G L V+DHNKKTG+NL+ V VVNGL +  F  P 
Sbjct: 1  MSIMSAF---KPIEDINDNLEVQD-LGNLTVDDHNKKTGENLKFVRVVNGLAAS-FSDPI 60

Query: 70 STHEGILYHLLVEAKTIEGINWTYVAKL 98
          S   G+LY L++EA T   INWTY A++
Sbjct: 61 SPLGGVLYELVLEAITTSKINWTYKARV 83

The following BLAST results are available for this feature:
BLAST of CsaV3_4G030360 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_031739698.11.8e-68100.00uncharacterized protein LOC116403239 [Cucumis sativus] >KAE8649718.1 hypothetica... [more]
TYK05428.14.1e-4492.86Proteinase inhibitor I25, cystatin [Cucumis melo var. makuwa][more]
KGN54823.13.4e-1454.74hypothetical protein Csa_012607 [Cucumis sativus][more]
XP_008442277.15.8e-1454.55PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cys... [more]
KAE8649716.17.8e-1151.58hypothetical protein Csa_012476 [Cucumis sativus][more]
BLAST of CsaV3_4G030360 vs. NCBI nr
Match NameE-valueIdentityDescription
BLAST of CsaV3_4G030360 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L3W11.4e-53100.00Proteinase inhibitor I25, cystatin OS=Cucumis sativus OX=3659 GN=Csa_4G508490 PE... [more]
A0A5D3C2L12.0e-4492.86Proteinase inhibitor I25, cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A0A0L1911.2e-1758.16Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE... [more]
A0A0A0KZ981.6e-1454.74Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508510 PE=4 SV=1[more]
A0A5A7TJK02.8e-1454.55Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
BLAST of CsaV3_4G030360 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.450.10coord: 18..103
e-value: 2.7E-9
score: 38.9
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 22..99

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_4G030360.1CsaV3_4G030360.1mRNA