CsaV3_4G028870 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_4G028870
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionCytochrome P450
Locationchr4: 18349295 .. 18351918 (+)
RNA-Seq ExpressionCsaV3_4G028870
SyntenyCsaV3_4G028870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCTTAGCCATGGTGATTGTTGGTCTGTTTTTACTCCTCTGTTTTTGCGCTGCTTTACTCCGACGGAATGAAGTTCGATACATAAAAAATGGATTGCCTCCTGGTACAATGGGTTGGCCTGTTTTTGGTGAAACCTCTGAGTTTCTTAAACAAGGTCCGAATTTTATGAATTCTAAAAGAGCAAGGTACTGATTAAAAATTTACTCTGTCTTTCCTTTTTCTTTTTTATTTTCTTTTGCCTTTAGATTTCTTCGTAATCTCTGGTTTTAACGTTTGCTGTCTTTTGTGAAGGTATGGGAGCTTTTTCAAATCACATATTTTGGGATCTCCTACGGTTGTTTCTATGGATCCAGAGGTTAATAGATATATTTTAATGAATGAATCTAAAGGACTCGTCGCTGGTTATCCTCAATCTATGCTTGATATACTTGGAAAATGCAATATCGCTGCCGTACACGGCGACGCTCATAAGCTTATGAGAGGTGCTCTACTTGCTATTGTTAGTCCCACCATGATTAAAGATCGTCTTCTTCTTCAAGTTGATGAGTTTATGAGATCCCATTTGCGTAATTGGGATAGCCAAATCATTGATATTCAAGAAAAAACTAAACAGGTGTATTTTAGTTTTTTAAAATACTGCAAAAATTTTCTCTGTTTTCACTCATTTTTAACGTTGTCCTCCCTCCCCCTCTCTTCAGATGGCTTTCTGGACATCTTTGAAGCAGACTACCAGTATCGAATCCGGTCCATTATGGGAATCTTTCACCATAGAATTCTTTAAGCTCGTCCTGGGCACTTTCTCGCTCCCAATCGATCTTCCAGGCACGAATTACCGTATAGGAGTTCAGGCAAGGAATAACATAGTAAAGCTATTGAGGCAGCTGATTAATGAGAGAGGAAATTCAAAGATTCAAAAAGATGACATGCTTGGATATTTATTGAGTGAAGAGAACAAATATAAACTAAATGAGGAACAGATTATTGATCAAGTCATTACTGTTTTCTATTCTGGTTATGAAACTGTTTCAACAACTTCAATGATGGCTGTAAAGTTCCTTCATGATCATCCCAAAGTTCTTCAGCAACTCAGAGTGAGTATTAAAATAAGAAAAAAAAAATTATAACTCATTGAGTTGGAAATTTGAAAAAGCTGTTGGTTCTTTTTAAAAAAATTCATTTCGAAGTTAAGAGATTTGAAATTTGCAGGAAGAACATTTAGCATTAAGAGAGAAGAAAAATCCCAAGGACCCGATCGATTGGGATGATTTTAAAGCAATGGAGTTCACTCGTGCGGTATGATTTTTTTATGTTTCTACTGTCATTTTTTAACAGTTCTTTGATGGGTTTCTTTAAAAAAAATTATCAAGATTCTAACTTTACACTGTTTTGTTTTGGAATTGTTTAGGTAATTTTTGAAACATCAAGGCTAGCTACTGTTGTTAATGGAGTTTTGAGGAAAACTACAAAGGATTTGGAAGTAAATGGTGAGTTTATTAGTACTTTTTCTTCAAAAGTATTTTGGTAACAAATTTAAACATTGAAAGGATAATGGTTCAAGGGTTTTGGTATATGTATATAAAAGTAATTTTAAAAGTATCAGTCAGTTTTAAAACAAGCTGCAAAGTTGCATGGTAGGCAAGTTTTACTTTAGAGTTATTTGCAGTAACCAATATTCATCTCCCCAAATCACTTCAAGCCTCGTTCCTGTTTTTGCAGTTACCAATTAACTTTGGGCCACTTTTTCTTTCTTTCTCTCTCTTCTCTGTTTTTTCTTTTAAGTCAGCCTTAGGCTCATAAATAATGACGGAACTTCTTCACTCCATTGGTTACAAGTGTTTTTTTCTATAAAATGAATCCAAACCTATATATAACCAAAGAAATATACAGTTTTGAAATAATATCATAATAATATCTAAAAATAATCTAATTACGTAAACAACAACAATGTGGTGAGATCAAACAAAAAAGTTTCAATCTTTAACTGTTTTATAAAATAAAATTATTTTACTTTTTACAGGGTTTCTAATTCCCAAGGGATGGAGGATTTATGTGTATAATCGAGAGATAAACTATGACCCTTTTCTCTATTCCGAACCATACACTTTCAACCCTTGGAGATGGCTGGTGGATCTCTCTCTCTCTAACTTTCTCTCTCTCTCTCTTACCTTTGTTGAAAAATGACCATTTTGCTCTTGTTTAACATTTTTCAGGACAAAAGCTTAGAGTCCCATAATTATTTTTTCCTCTTTGGCGGTGGAATTAGGCTTTGTCCTGGCAAAGAATTGGGAATTGCAGAGGTTTCTACATTTTTACATTACTTTGTAACTAAATACAGGTACGTAGCTTTCTTTTCCATATTTTATGTATTCTTTTTACTTTTAACTGCTATTTTGATATGCACATTTTTGTCAGTCTCAAATTCCGATTGTATATTTTGTTGGATACGTAAAGATAGGTATGATCCCACATGCACTAAATCCAAAATAAAAACAGAAATTGTGGTAAAGAATTTTTGTTTTGTTTTCTGTTGTAGGTGGGAAGAAGTTGGAGAATGTAAGCTCTTGAAATTTCCAAGAGTTAAAGCTCGGAATGGGTTTCACATTAGGGTTTCATCCTACTGA

mRNA sequence

ATGGGCTTAGCCATGGTGATTGTTGGTCTGTTTTTACTCCTCTGTTTTTGCGCTGCTTTACTCCGACGGAATGAAGTTCGATACATAAAAAATGGATTGCCTCCTGGTACAATGGGTTGGCCTGTTTTTGGTGAAACCTCTGAGTTTCTTAAACAAGGTCCGAATTTTATGAATTCTAAAAGAGCAAGGTATGGGAGCTTTTTCAAATCACATATTTTGGGATCTCCTACGGTTGTTTCTATGGATCCAGAGGTTAATAGATATATTTTAATGAATGAATCTAAAGGACTCGTCGCTGGTTATCCTCAATCTATGCTTGATATACTTGGAAAATGCAATATCGCTGCCGTACACGGCGACGCTCATAAGCTTATGAGAGGTGCTCTACTTGCTATTGTTAGTCCCACCATGATTAAAGATCGTCTTCTTCTTCAAGTTGATGAGTTTATGAGATCCCATTTGCGTAATTGGGATAGCCAAATCATTGATATTCAAGAAAAAACTAAACAGATGGCTTTCTGGACATCTTTGAAGCAGACTACCAGTATCGAATCCGGTCCATTATGGGAATCTTTCACCATAGAATTCTTTAAGCTCGTCCTGGGCACTTTCTCGCTCCCAATCGATCTTCCAGGCACGAATTACCGTATAGGAGTTCAGGCAAGGAATAACATAGTAAAGCTATTGAGGCAGCTGATTAATGAGAGAGGAAATTCAAAGATTCAAAAAGATGACATGCTTGGATATTTATTGAGTGAAGAGAACAAATATAAACTAAATGAGGAACAGATTATTGATCAAGTCATTACTGTTTTCTATTCTGGTTATGAAACTGTTTCAACAACTTCAATGATGGCTGTAAAGTTCCTTCATGATCATCCCAAAGTTCTTCAGCAACTCAGAGAAGAACATTTAGCATTAAGAGAGAAGAAAAATCCCAAGGACCCGATCGATTGGGATGATTTTAAAGCAATGGAGTTCACTCGTGCGGTAATTTTTGAAACATCAAGGCTAGCTACTGTTGTTAATGGAGTTTTGAGGAAAACTACAAAGGATTTGGAAGTAAATGGGTTTCTAATTCCCAAGGGATGGAGGATTTATGACAAAAGCTTAGAGTCCCATAATTATTTTTTCCTCTTTGGCGGTGGAATTAGGCTTTGTCCTGGCAAAGAATTGGGAATTGCAGAGGTTTCTACATTTTTACATTACTTTGTAACTAAATACAGGTGGGAAGAAGTTGGAGAATGTAAGCTCTTGAAATTTCCAAGAGTTAAAGCTCGGAATGGGTTTCACATTAGGGTTTCATCCTACTGA

Coding sequence (CDS)

ATGGGCTTAGCCATGGTGATTGTTGGTCTGTTTTTACTCCTCTGTTTTTGCGCTGCTTTACTCCGACGGAATGAAGTTCGATACATAAAAAATGGATTGCCTCCTGGTACAATGGGTTGGCCTGTTTTTGGTGAAACCTCTGAGTTTCTTAAACAAGGTCCGAATTTTATGAATTCTAAAAGAGCAAGGTATGGGAGCTTTTTCAAATCACATATTTTGGGATCTCCTACGGTTGTTTCTATGGATCCAGAGGTTAATAGATATATTTTAATGAATGAATCTAAAGGACTCGTCGCTGGTTATCCTCAATCTATGCTTGATATACTTGGAAAATGCAATATCGCTGCCGTACACGGCGACGCTCATAAGCTTATGAGAGGTGCTCTACTTGCTATTGTTAGTCCCACCATGATTAAAGATCGTCTTCTTCTTCAAGTTGATGAGTTTATGAGATCCCATTTGCGTAATTGGGATAGCCAAATCATTGATATTCAAGAAAAAACTAAACAGATGGCTTTCTGGACATCTTTGAAGCAGACTACCAGTATCGAATCCGGTCCATTATGGGAATCTTTCACCATAGAATTCTTTAAGCTCGTCCTGGGCACTTTCTCGCTCCCAATCGATCTTCCAGGCACGAATTACCGTATAGGAGTTCAGGCAAGGAATAACATAGTAAAGCTATTGAGGCAGCTGATTAATGAGAGAGGAAATTCAAAGATTCAAAAAGATGACATGCTTGGATATTTATTGAGTGAAGAGAACAAATATAAACTAAATGAGGAACAGATTATTGATCAAGTCATTACTGTTTTCTATTCTGGTTATGAAACTGTTTCAACAACTTCAATGATGGCTGTAAAGTTCCTTCATGATCATCCCAAAGTTCTTCAGCAACTCAGAGAAGAACATTTAGCATTAAGAGAGAAGAAAAATCCCAAGGACCCGATCGATTGGGATGATTTTAAAGCAATGGAGTTCACTCGTGCGGTAATTTTTGAAACATCAAGGCTAGCTACTGTTGTTAATGGAGTTTTGAGGAAAACTACAAAGGATTTGGAAGTAAATGGGTTTCTAATTCCCAAGGGATGGAGGATTTATGACAAAAGCTTAGAGTCCCATAATTATTTTTTCCTCTTTGGCGGTGGAATTAGGCTTTGTCCTGGCAAAGAATTGGGAATTGCAGAGGTTTCTACATTTTTACATTACTTTGTAACTAAATACAGGTGGGAAGAAGTTGGAGAATGTAAGCTCTTGAAATTTCCAAGAGTTAAAGCTCGGAATGGGTTTCACATTAGGGTTTCATCCTACTGA

Protein sequence

MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSKRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGDAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQTTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSKIQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQLREEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLIPKGWRIYDKSLESHNYFFLFGGGIRLCPGKELGIAEVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY*
Homology
BLAST of CsaV3_4G028870 vs. NCBI nr
Match: KAE8649648.1 (hypothetical protein Csa_012605 [Cucumis sativus])

HSP 1 Score: 884.4 bits (2284), Expect = 4.0e-253
Identity = 437/437 (100.00%), Postives = 437/437 (100.00%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK
Sbjct: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD
Sbjct: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120

Query: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT
Sbjct: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240
           TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK
Sbjct: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360
           REEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI
Sbjct: 301 REEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360

Query: 361 PKGWRIYDKSLESHNYFFLFGGGIRLCPGKELGIAEVSTFLHYFVTKYRWEEVGECKLLK 420
           PKGWRIYDKSLESHNYFFLFGGGIRLCPGKELGIAEVSTFLHYFVTKYRWEEVGECKLLK
Sbjct: 361 PKGWRIYDKSLESHNYFFLFGGGIRLCPGKELGIAEVSTFLHYFVTKYRWEEVGECKLLK 420

Query: 421 FPRVKARNGFHIRVSSY 438
           FPRVKARNGFHIRVSSY
Sbjct: 421 FPRVKARNGFHIRVSSY 437

BLAST of CsaV3_4G028870 vs. NCBI nr
Match: XP_004150514.3 (cytochrome P450 85A [Cucumis sativus])

HSP 1 Score: 870.5 bits (2248), Expect = 6.0e-249
Identity = 437/462 (94.59%), Postives = 437/462 (94.59%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK
Sbjct: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD
Sbjct: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120

Query: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT
Sbjct: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240
           TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK
Sbjct: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360
           REEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI
Sbjct: 301 REEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360

Query: 361 PKGWRIY-------------------------DKSLESHNYFFLFGGGIRLCPGKELGIA 420
           PKGWRIY                         DKSLESHNYFFLFGGGIRLCPGKELGIA
Sbjct: 361 PKGWRIYVYNREINYDPFLYSEPYTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGIA 420

Query: 421 EVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 438
           EVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY
Sbjct: 421 EVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 462

BLAST of CsaV3_4G028870 vs. NCBI nr
Match: XP_008455791.1 (PREDICTED: cytochrome P450 85A-like [Cucumis melo])

HSP 1 Score: 827.0 bits (2135), Expect = 7.6e-236
Identity = 413/462 (89.39%), Postives = 425/462 (91.99%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           M LA+VI+ LFLLLCFCAAL + NEVRY KNGLPPGTMGWPVFGETSEFLKQGPNFMNSK
Sbjct: 1   MALAIVILALFLLLCFCAALFQWNEVRYRKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGSFFKSHILGSPTV+SMDPEVNRYILMNESKGLVAGYPQSMLDILG CNIAAVHGD
Sbjct: 61  RARYGSFFKSHILGSPTVISMDPEVNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHGD 120

Query: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIV PTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT
Sbjct: 121 AHKLMRGALLAIVGPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240
           TSI SGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLL++LI+ER NSK
Sbjct: 181 TSIVSGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKKLISERVNSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQKDDMLGYLLSEENKYKLNEEQIIDQ+ITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQIITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360
           REEH+ALREKKNP+DPIDWDDFKAM+FTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI
Sbjct: 301 REEHIALREKKNPEDPIDWDDFKAMDFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360

Query: 361 PKGWRIY-------------------------DKSLESHNYFFLFGGGIRLCPGKELGIA 420
           PKGWRIY                         DK+LESHNYFFLFGGG RLCPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGIA 420

Query: 421 EVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 438
           EVSTFLHYFVTKYRWEEVG CKLLKFPRVKA NGFHIRVSSY
Sbjct: 421 EVSTFLHYFVTKYRWEEVGGCKLLKFPRVKAPNGFHIRVSSY 462

BLAST of CsaV3_4G028870 vs. NCBI nr
Match: XP_038890036.1 (cytochrome P450 85A-like [Benincasa hispida])

HSP 1 Score: 758.4 bits (1957), Expect = 3.3e-215
Identity = 377/462 (81.60%), Postives = 407/462 (88.10%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           M LAMVI+ LFLLLCFC+ALLR NEVRY K GLPPGTMGWPVFGET+EFLKQGPNFM S+
Sbjct: 1   MALAMVILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGS FKSHILG PT+VSMDPEVNRY+LMNESKGLV GYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIVSPTMIKDRLL +VD+FMRSHLRNWDSQIIDIQEKTK+MA  +SLKQ 
Sbjct: 121 AHKLMRGALLAIVSPTMIKDRLLPKVDDFMRSHLRNWDSQIIDIQEKTKEMALRSSLKQI 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240
             IESGPL ESF  EFFKLVLGT SLPIDLPGTNYR G+QAR NI+KLL+QLINER +SK
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRDSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQKDDMLG+L+SEENKYKLN+E+IIDQ+ITV YSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKDDMLGFLMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360
           REEHLA+REKKNP+DPIDWDD KAM+FTRAVIFETSRLAT+VNGVLRKTTKD+E+NGFLI
Sbjct: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIY-------------------------DKSLESHNYFFLFGGGIRLCPGKELGIA 420
           PKGWRIY                         DK+LESHNYFF+FGGG R CPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420

Query: 421 EVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 438
           E+STFLHYFVTKYRWEEVG  KLLKFPRV+A +GFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 462

BLAST of CsaV3_4G028870 vs. NCBI nr
Match: KAA0040382.1 (cytochrome P450 85A-like [Cucumis melo var. makuwa] >TYK04049.1 cytochrome P450 85A-like [Cucumis melo var. makuwa])

HSP 1 Score: 748.8 bits (1932), Expect = 2.6e-212
Identity = 376/428 (87.85%), Postives = 390/428 (91.12%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           M LA+VI+ LFLLLCFCAAL + NEVRY KNGLPPGTMGWPVFGETSEFLKQGPNFMNSK
Sbjct: 1   MALAIVILALFLLLCFCAALFQWNEVRYRKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGSFFKSHILGSPTV+SMDPEVNRYILMNESKGLVAGYPQSMLDILG CNIAAVHGD
Sbjct: 61  RARYGSFFKSHILGSPTVISMDPEVNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHGD 120

Query: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIV PTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT
Sbjct: 121 AHKLMRGALLAIVGPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240
           TSI SGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLL++LI+ER NSK
Sbjct: 181 TSIVSGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKKLISERVNSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQKDDMLGYLLSEENKYKLNEEQIIDQ+ITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQIITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360
           REEH+ALREKKNP+DPIDWDDFKAM+FTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI
Sbjct: 301 REEHIALREKKNPEDPIDWDDFKAMDFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360

Query: 361 PKGWRIY-------------------------DKSLESHNYFFLFGGGIRLCPGKELGIA 404
           PKGWRIY                         DK+LESHNYFFLFGGG RLCPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGIA 420

BLAST of CsaV3_4G028870 vs. ExPASy Swiss-Prot
Match: Q69F95 (Cytochrome P450 85A OS=Phaseolus vulgaris OX=3885 GN=BA13 PE=3 SV=2)

HSP 1 Score: 627.1 bits (1616), Expect = 1.5e-178
Identity = 311/464 (67.03%), Postives = 375/464 (80.82%), Query Frame = 0

Query: 1   MGLAMVIVG-LFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           M L M I+G L LLLCFC+ALL+ NEVR+ + GLPPG MGWPVFGET+EFLKQGPNFM +
Sbjct: 1   MALFMAILGVLVLLLCFCSALLKWNEVRFRRKGLPPGAMGWPVFGETTEFLKQGPNFMKN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           KRARYGSFFKSHILG PT+VSMDPE+NR+ILMNE+KGLV GYPQSMLDILG  NIAAVHG
Sbjct: 61  KRARYGSFFKSHILGCPTIVSMDPELNRFILMNEAKGLVPGYPQSMLDILGTRNIAAVHG 120

Query: 121 DAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQ 180
             HK MRGALL+I+SPT+I+D+LL ++DEFMR+HL +WD+++I+IQEKTK+MAF +SLKQ
Sbjct: 121 STHKYMRGALLSIISPTLIRDQLLPKIDEFMRTHLMDWDNKVINIQEKTKEMAFLSSLKQ 180

Query: 181 TTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNS 240
              +ES  + + F  EFFKLVLGT SLPI+LP TNYR G+QAR +I+ +L QL+ ER  S
Sbjct: 181 IAGMESSSIAQPFMTEFFKLVLGTLSLPINLPRTNYRGGLQARKSIISILSQLLEERKAS 240

Query: 241 KIQKDDMLGYLL-SEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQ 300
           +    DMLG L+  +EN+YKL++E+IID VIT+ YSGYETVSTTSMMAVK+LHDHPKVL+
Sbjct: 241 QDVHVDMLGCLMKKDENRYKLSDEEIIDLVITIMYSGYETVSTTSMMAVKYLHDHPKVLE 300

Query: 301 QLREEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGF 360
           ++R+EH A+RE+K P+DPID +D K+M FTRAVIFETSRLAT+VNGVLRKTT D+E+NG+
Sbjct: 301 EIRKEHFAIRERKKPEDPIDCNDLKSMRFTRAVIFETSRLATIVNGVLRKTTHDMELNGY 360

Query: 361 LIPKGWRIY-------------------------DKSLESHNYFFLFGGGIRLCPGKELG 420
           LIPKGWRIY                           SLES ++F +FGGG R CPGKELG
Sbjct: 361 LIPKGWRIYVYTREINYDPFLYHDPLTFNPWRWLGNSLESQSHFLIFGGGTRQCPGKELG 420

Query: 421 IAEVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 438
           IAE+STFLHYFVT+YRWEEVG  KL+KFPRV A NG HIRVSS+
Sbjct: 421 IAEISTFLHYFVTRYRWEEVGGDKLMKFPRVVAPNGLHIRVSSF 464

BLAST of CsaV3_4G028870 vs. ExPASy Swiss-Prot
Match: Q43147 (Cytochrome P450 85A1 OS=Solanum lycopersicum OX=4081 GN=CYP85A1 PE=2 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 4.5e-175
Identity = 302/464 (65.09%), Postives = 370/464 (79.74%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLC-FCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           M   ++ +  F  LC FC ALLR N+V+Y +  LPPGTMGWP+FGET+EFLK GP+FM +
Sbjct: 1   MAFFLIFLSSFFGLCIFCTALLRWNQVKYNQKNLPPGTMGWPLFGETTEFLKLGPSFMKN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           +RARYGSFFKSHILG PT+VSMD E+NRYIL+NE+KGLV GYPQSM+DILGKCNIAAV+G
Sbjct: 61  QRARYGSFFKSHILGCPTIVSMDSELNRYILVNEAKGLVPGYPQSMIDILGKCNIAAVNG 120

Query: 121 DAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQ 180
            AHK MRGALL+++SPTMI+D+LL ++DEFMRSHL NWD+++IDIQEKT +MAF +SLKQ
Sbjct: 121 SAHKYMRGALLSLISPTMIRDQLLPKIDEFMRSHLTNWDNKVIDIQEKTNKMAFLSSLKQ 180

Query: 181 TTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNS 240
              IES  L + F  EFF LVLGT SLPI+LP TNY  G QAR  IV LLR LI ER  S
Sbjct: 181 IAGIESTSLAQEFMSEFFNLVLGTLSLPINLPNTNYHRGFQARKIIVNLLRTLIEERRAS 240

Query: 241 KIQKDDMLGYLLSEE-NKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQ 300
           K  + DMLGYL++EE  ++KL ++++ID +IT+ YSGYETVSTTSMMAVK+LHDHPKVL+
Sbjct: 241 KEIQHDMLGYLMNEEATRFKLTDDEMIDLIITILYSGYETVSTTSMMAVKYLHDHPKVLE 300

Query: 301 QLREEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGF 360
           +LR+EH+A+REKK P+DPID++D+++M FTRAVI ETSRLAT+VNGVLRKTT+D+E+NG+
Sbjct: 301 ELRKEHMAIREKKKPEDPIDYNDYRSMRFTRAVILETSRLATIVNGVLRKTTQDMEINGY 360

Query: 361 LIPKGWRIY-------------------------DKSLESHNYFFLFGGGIRLCPGKELG 420
           +IPKGWRIY                         DKSLE  N F +FGGG R CPGKELG
Sbjct: 361 IIPKGWRIYVYTRELNYDPRLYPDPYSFNPWRWMDKSLEHQNSFLVFGGGTRQCPGKELG 420

Query: 421 IAEVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 438
           +AE+STFLHYFVTKYRWEE+G  KL+KFPRV+A NG  IRVS++
Sbjct: 421 VAEISTFLHYFVTKYRWEEIGGDKLMKFPRVEAPNGLRIRVSAH 464

BLAST of CsaV3_4G028870 vs. ExPASy Swiss-Prot
Match: Q940V4 (Cytochrome P450 85A2 OS=Arabidopsis thaliana OX=3702 GN=CYP85A2 PE=2 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 3.8e-174
Identity = 301/465 (64.73%), Postives = 368/465 (79.14%), Query Frame = 0

Query: 1   MGLAMVIVGLF-LLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           MG+ M+I+GL  +++C C ALLR N++RY K GLPPGTMGWP+FGET+EFLKQGP+FM +
Sbjct: 1   MGIMMMILGLLVIIVCLCTALLRWNQMRYSKKGLPPGTMGWPIFGETTEFLKQGPDFMKN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           +R RYGSFFKSHILG PT+VSMD E+NRYILMNESKGLVAGYPQSMLDILG CNIAAVHG
Sbjct: 61  QRLRYGSFFKSHILGCPTIVSMDAELNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHG 120

Query: 121 DAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLK 180
            +H+LMRG+LL+++SPTM+KD LL ++D+FMR++L  WD  + +DIQEKTK MAF +SL 
Sbjct: 121 PSHRLMRGSLLSLISPTMMKDHLLPKIDDFMRNYLCGWDDLETVDIQEKTKHMAFLSSLL 180

Query: 181 QTTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGN 240
           Q       P  E +  EFFKLV+GT S+PID+PGTNYR GVQARNNI +LL +L+ ER  
Sbjct: 181 QIAETLKKPEVEEYRTEFFKLVVGTLSVPIDIPGTNYRSGVQARNNIDRLLTELMQERKE 240

Query: 241 SKIQKDDMLGYLL-SEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLGYL+  E+N+Y L +++I DQV+T+ YSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEDNRYLLTDKEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQLREEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNG 360
           ++LR EHLA+RE+K P +P+  DD K+M+FTRAVIFETSRLAT+VNGVLRKTT DLE+NG
Sbjct: 301 EELRREHLAIRERKRPDEPLTLDDIKSMKFTRAVIFETSRLATIVNGVLRKTTHDLELNG 360

Query: 361 FLIPKGWRIY-------------------------DKSLESHNYFFLFGGGIRLCPGKEL 420
           +LIPKGWRIY                         +KSLES +YF LFGGG+RLCPGKEL
Sbjct: 361 YLIPKGWRIYVYTREINYDTSLYEDPMIFNPWRWMEKSLESKSYFLLFGGGVRLCPGKEL 420

Query: 421 GIAEVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 438
           GI+EVS+FLHYFVTKYRWEE GE KL+ FPRV A  G+H++ S Y
Sbjct: 421 GISEVSSFLHYFVTKYRWEENGEDKLMVFPRVSAPKGYHLKCSPY 465

BLAST of CsaV3_4G028870 vs. ExPASy Swiss-Prot
Match: Q9FMA5 (Cytochrome P450 85A1 OS=Arabidopsis thaliana OX=3702 GN=CYP85A1 PE=2 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 3.7e-169
Identity = 294/465 (63.23%), Postives = 362/465 (77.85%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLL-CFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           MG  MV++GL L++   C+ALLR N++RY KNGLPPGTMGWP+FGET+EFLKQGPNFM +
Sbjct: 1   MGAMMVMMGLLLIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLKQGPNFMRN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           +R RYGSFFKSH+LG PT++SMD EVNRYIL NESKGLV GYPQSMLDILG CN+AAVHG
Sbjct: 61  QRLRYGSFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHG 120

Query: 121 DAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLK 180
            +H+LMRG+LL+++S TM++D +L +VD FMRS+L  W+  ++IDIQ+KTK MAF +SL 
Sbjct: 121 SSHRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLT 180

Query: 181 QTTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGN 240
           Q       P  E F   FFKLV+GT S+PIDLPGTNYR G+QARNNI +LLR+L+ ER +
Sbjct: 181 QIAGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRD 240

Query: 241 SKIQKDDMLGYLLSEE-NKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLGYL+ +E N+Y L +E+I DQV+T+ YSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQLREEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNG 360
           Q+LR EHLA RE+K   +P+  +D K+M+FTRAVI+ETSRLAT+VNGVLRKTT+DLE+NG
Sbjct: 301 QELRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEING 360

Query: 361 FLIPKGWRIY-------------------------DKSLESHNYFFLFGGGIRLCPGKEL 420
           +LIPKGWRIY                          KSLES N  F+FGGG RLCPGKEL
Sbjct: 361 YLIPKGWRIYVYTREINYDANLYEDPLIFNPWRWMKKSLESQNSCFVFGGGTRLCPGKEL 420

Query: 421 GIAEVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 438
           GI E+S+FLHYFVT+YRWEE+G  +L+ FPRV A  GFH+R+S Y
Sbjct: 421 GIVEISSFLHYFVTRYRWEEIGGDELMVFPRVFAPKGFHLRISPY 465

BLAST of CsaV3_4G028870 vs. ExPASy Swiss-Prot
Match: Q50LE0 (Cytochrome P450 85A3 OS=Solanum lycopersicum OX=4081 GN=CYP85A3 PE=2 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 1.9e-157
Identity = 275/467 (58.89%), Postives = 353/467 (75.59%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAA-LLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           M + ++I  +F   C  +  L R  ++ Y K  LPPGTMGWP+FGET EFL QGPNFM +
Sbjct: 1   MAIFLIIFVVFFGFCILSTPLFRWIDIVYNKKNLPPGTMGWPIFGETREFLNQGPNFMKN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           +RARYG+FFKSHILG PTVVSMD  +N YIL NE+KGL+ GYPQSMLDILGKCNIAAVHG
Sbjct: 61  QRARYGNFFKSHILGCPTVVSMDAGLNVYILNNEAKGLIPGYPQSMLDILGKCNIAAVHG 120

Query: 121 DAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLK 180
             HK +RGALL++++PTMIKD +L ++D+FMRSHL  WD+  +IDIQ+ TK+MAF++SL 
Sbjct: 121 ATHKYIRGALLSLINPTMIKDHILPKIDKFMRSHLSGWDNCNVIDIQQMTKEMAFFSSLD 180

Query: 181 QTTSI-ESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERG 240
           Q      S  + + F   F  + LGT SLPI+ P TNY  G+Q R  IVKLLR++I +R 
Sbjct: 181 QIGGFATSSSIAQEFRAGFLNIALGTISLPINFPTTNYYRGLQGRKTIVKLLRKIIEDRR 240

Query: 241 NSKIQKDDMLGYLLSEE--NKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPK 300
            SK  + DMLG +++EE  N+Y L++E++IDQ+IT+ YSG+ETVSTTSMMAVK+LHDHPK
Sbjct: 241 GSKKIQQDMLGLMMNEEAKNRYTLSDEELIDQIITIMYSGFETVSTTSMMAVKYLHDHPK 300

Query: 301 VLQQLREEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEV 360
            L+++R+EH A+REKK+ +DPID++DFKAM FTRAVI+ET RLAT+VNGVLRKTT+D+E+
Sbjct: 301 ALEEIRKEHFAIREKKSLEDPIDYNDFKAMRFTRAVIYETLRLATIVNGVLRKTTQDMEL 360

Query: 361 NGFLIPKGWRIY-------------------------DKSLESHNYFFLFGGGIRLCPGK 420
           NG++IPKGWRIY                         + +L+  + F +FGGG RLCPGK
Sbjct: 361 NGYMIPKGWRIYVYTRELNYDPLIYPDPYTFNPWRWLENNLDHQSSFLMFGGGTRLCPGK 420

Query: 421 ELGIAEVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 438
           ELG+AE+STFLHYFVT+YRWEEVG  KL+KFPRV+A NG  I+VS+Y
Sbjct: 421 ELGVAEISTFLHYFVTRYRWEEVGGNKLMKFPRVEALNGLWIKVSAY 467

BLAST of CsaV3_4G028870 vs. ExPASy TrEMBL
Match: A0A0A0L0Q5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G410870 PE=3 SV=1)

HSP 1 Score: 860.5 bits (2222), Expect = 3.0e-246
Identity = 432/458 (94.32%), Postives = 432/458 (94.32%), Query Frame = 0

Query: 5   MVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSKRARY 64
           MVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSKRARY
Sbjct: 1   MVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSKRARY 60

Query: 65  GSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGDAHKL 124
           GSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGDAHKL
Sbjct: 61  GSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGDAHKL 120

Query: 125 MRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQTTSIE 184
           MRGALLAIVS TMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQTTSIE
Sbjct: 121 MRGALLAIVSHTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQTTSIE 180

Query: 185 SGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSKIQKD 244
           SGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSKIQKD
Sbjct: 181 SGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSKIQKD 240

Query: 245 DMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQLREEH 304
           DMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQLREEH
Sbjct: 241 DMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQLREEH 300

Query: 305 LALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLIPKGW 364
           LALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLIPKGW
Sbjct: 301 LALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLIPKGW 360

Query: 365 RIY-------------------------DKSLESHNYFFLFGGGIRLCPGKELGIAEVST 424
           RIY                         DKSLESHNYFFLFGGGIRLCPGKELGIAEVST
Sbjct: 361 RIYVYNREINYDPFLYSEPYTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGIAEVST 420

Query: 425 FLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 438
           FLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY
Sbjct: 421 FLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 458

BLAST of CsaV3_4G028870 vs. ExPASy TrEMBL
Match: A0A1S3C1A8 (cytochrome P450 85A-like OS=Cucumis melo OX=3656 GN=LOC103495886 PE=3 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 3.7e-236
Identity = 413/462 (89.39%), Postives = 425/462 (91.99%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           M LA+VI+ LFLLLCFCAAL + NEVRY KNGLPPGTMGWPVFGETSEFLKQGPNFMNSK
Sbjct: 1   MALAIVILALFLLLCFCAALFQWNEVRYRKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGSFFKSHILGSPTV+SMDPEVNRYILMNESKGLVAGYPQSMLDILG CNIAAVHGD
Sbjct: 61  RARYGSFFKSHILGSPTVISMDPEVNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHGD 120

Query: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIV PTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT
Sbjct: 121 AHKLMRGALLAIVGPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240
           TSI SGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLL++LI+ER NSK
Sbjct: 181 TSIVSGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKKLISERVNSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQKDDMLGYLLSEENKYKLNEEQIIDQ+ITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQIITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360
           REEH+ALREKKNP+DPIDWDDFKAM+FTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI
Sbjct: 301 REEHIALREKKNPEDPIDWDDFKAMDFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360

Query: 361 PKGWRIY-------------------------DKSLESHNYFFLFGGGIRLCPGKELGIA 420
           PKGWRIY                         DK+LESHNYFFLFGGG RLCPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGIA 420

Query: 421 EVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 438
           EVSTFLHYFVTKYRWEEVG CKLLKFPRVKA NGFHIRVSSY
Sbjct: 421 EVSTFLHYFVTKYRWEEVGGCKLLKFPRVKAPNGFHIRVSSY 462

BLAST of CsaV3_4G028870 vs. ExPASy TrEMBL
Match: A0A5A7TA97 (Cytochrome P450 85A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold460G00020 PE=3 SV=1)

HSP 1 Score: 748.8 bits (1932), Expect = 1.3e-212
Identity = 376/428 (87.85%), Postives = 390/428 (91.12%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           M LA+VI+ LFLLLCFCAAL + NEVRY KNGLPPGTMGWPVFGETSEFLKQGPNFMNSK
Sbjct: 1   MALAIVILALFLLLCFCAALFQWNEVRYRKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGSFFKSHILGSPTV+SMDPEVNRYILMNESKGLVAGYPQSMLDILG CNIAAVHGD
Sbjct: 61  RARYGSFFKSHILGSPTVISMDPEVNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHGD 120

Query: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIV PTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT
Sbjct: 121 AHKLMRGALLAIVGPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240
           TSI SGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLL++LI+ER NSK
Sbjct: 181 TSIVSGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKKLISERVNSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQKDDMLGYLLSEENKYKLNEEQIIDQ+ITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQIITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360
           REEH+ALREKKNP+DPIDWDDFKAM+FTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI
Sbjct: 301 REEHIALREKKNPEDPIDWDDFKAMDFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360

Query: 361 PKGWRIY-------------------------DKSLESHNYFFLFGGGIRLCPGKELGIA 404
           PKGWRIY                         DK+LESHNYFFLFGGG RLCPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGIA 420

BLAST of CsaV3_4G028870 vs. ExPASy TrEMBL
Match: A0A0A0KXL9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G641590 PE=3 SV=1)

HSP 1 Score: 741.1 bits (1912), Expect = 2.6e-210
Identity = 369/463 (79.70%), Postives = 401/463 (86.61%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           M LAMVI+ LFLLL FC+ALLR NEVRY K GLPPGTMGWP+FGET+EFLKQGPNFM S+
Sbjct: 1   MALAMVIIALFLLLLFCSALLRWNEVRYSKKGLPPGTMGWPIFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGS FKSHILG PT+VSMDPEVNRY+LMNESKGLV GYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGS 120

Query: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIVSPTMIKDRLL  +DEFMRSHL NWD+QIIDIQEKTK+MA  +S+KQ 
Sbjct: 121 AHKLMRGALLAIVSPTMIKDRLLPNIDEFMRSHLSNWDNQIIDIQEKTKEMALRSSMKQI 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240
              ESGPL ESFT EFFKLVLGT SLPIDLPGTNYR G+QAR NI+KLL QLI ER +SK
Sbjct: 181 AGFESGPLSESFTPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLEQLIKERRDSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQK+DMLGY +SEENKYKLN+E+IIDQ+ITV YSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKNDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLALRE-KKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFL 360
           REEHLA+RE KKNP+DPIDWDD KAM+FTRAVIFETSRLAT+VNGVLRKTTKD+E+NGFL
Sbjct: 301 REEHLAIREKKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFL 360

Query: 361 IPKGWRIY-------------------------DKSLESHNYFFLFGGGIRLCPGKELGI 420
           IPKGWRIY                         D +LES+NYFF+FGGG RLCPGKELGI
Sbjct: 361 IPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDNNLESNNYFFIFGGGTRLCPGKELGI 420

Query: 421 AEVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 438
           AE+STFLHYFVTKYRWEEVG  KLLKFPRV+A +GFHIRVSSY
Sbjct: 421 AEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463

BLAST of CsaV3_4G028870 vs. ExPASy TrEMBL
Match: A0A5A7TDB4 (Cytochrome P450 85A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G002300 PE=3 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 1.7e-209
Identity = 368/463 (79.48%), Postives = 401/463 (86.61%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           M LAMVI+ LFLLL FC+ALLR NEVRY K GLPPGTMGWP+FGET+EFLKQGPNFM S+
Sbjct: 1   MALAMVILALFLLLLFCSALLRWNEVRYSKKGLPPGTMGWPIFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGS FKSHILG PT+VSMDPEVNRY+LMNESKGLV GYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGS 120

Query: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIVSPTMIKDRLL ++DEFMRSHL NWD+QIIDIQEKTK+MA  +S+KQ 
Sbjct: 121 AHKLMRGALLAIVSPTMIKDRLLPKLDEFMRSHLSNWDNQIIDIQEKTKEMALRSSMKQI 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240
              ESGPL ESFT EFFKLVLGT SLPIDLPGTNYR G+QAR NI+KLL QLI ER +SK
Sbjct: 181 AGFESGPLSESFTPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLEQLIKERRDSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQK+DMLGY +SEENKYKLN+E+IIDQ+ITV YSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKNDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLALRE-KKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFL 360
           REEHLA+RE KKNP+DPIDWDD KAM+FTRAVIFETSRLAT+VNGVLRKTTKD+E+NGFL
Sbjct: 301 REEHLAIREKKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFL 360

Query: 361 IPKGWRIY-------------------------DKSLESHNYFFLFGGGIRLCPGKELGI 420
           IPKGWRIY                         D +LES+NYFF+FGGG RLCPGKELGI
Sbjct: 361 IPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDNNLESNNYFFIFGGGTRLCPGKELGI 420

Query: 421 AEVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 438
            E+STFLHYFVTKYRWEEVG  KLLKFPRV+A +GFHIRVSSY
Sbjct: 421 TEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463

BLAST of CsaV3_4G028870 vs. TAIR 10
Match: AT3G30180.1 (brassinosteroid-6-oxidase 2 )

HSP 1 Score: 612.5 bits (1578), Expect = 2.7e-175
Identity = 301/465 (64.73%), Postives = 368/465 (79.14%), Query Frame = 0

Query: 1   MGLAMVIVGLF-LLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           MG+ M+I+GL  +++C C ALLR N++RY K GLPPGTMGWP+FGET+EFLKQGP+FM +
Sbjct: 1   MGIMMMILGLLVIIVCLCTALLRWNQMRYSKKGLPPGTMGWPIFGETTEFLKQGPDFMKN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           +R RYGSFFKSHILG PT+VSMD E+NRYILMNESKGLVAGYPQSMLDILG CNIAAVHG
Sbjct: 61  QRLRYGSFFKSHILGCPTIVSMDAELNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHG 120

Query: 121 DAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLK 180
            +H+LMRG+LL+++SPTM+KD LL ++D+FMR++L  WD  + +DIQEKTK MAF +SL 
Sbjct: 121 PSHRLMRGSLLSLISPTMMKDHLLPKIDDFMRNYLCGWDDLETVDIQEKTKHMAFLSSLL 180

Query: 181 QTTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGN 240
           Q       P  E +  EFFKLV+GT S+PID+PGTNYR GVQARNNI +LL +L+ ER  
Sbjct: 181 QIAETLKKPEVEEYRTEFFKLVVGTLSVPIDIPGTNYRSGVQARNNIDRLLTELMQERKE 240

Query: 241 SKIQKDDMLGYLL-SEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLGYL+  E+N+Y L +++I DQV+T+ YSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEDNRYLLTDKEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQLREEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNG 360
           ++LR EHLA+RE+K P +P+  DD K+M+FTRAVIFETSRLAT+VNGVLRKTT DLE+NG
Sbjct: 301 EELRREHLAIRERKRPDEPLTLDDIKSMKFTRAVIFETSRLATIVNGVLRKTTHDLELNG 360

Query: 361 FLIPKGWRIY-------------------------DKSLESHNYFFLFGGGIRLCPGKEL 420
           +LIPKGWRIY                         +KSLES +YF LFGGG+RLCPGKEL
Sbjct: 361 YLIPKGWRIYVYTREINYDTSLYEDPMIFNPWRWMEKSLESKSYFLLFGGGVRLCPGKEL 420

Query: 421 GIAEVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 438
           GI+EVS+FLHYFVTKYRWEE GE KL+ FPRV A  G+H++ S Y
Sbjct: 421 GISEVSSFLHYFVTKYRWEENGEDKLMVFPRVSAPKGYHLKCSPY 465

BLAST of CsaV3_4G028870 vs. TAIR 10
Match: AT5G38970.1 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 595.9 bits (1535), Expect = 2.6e-170
Identity = 294/465 (63.23%), Postives = 362/465 (77.85%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLL-CFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           MG  MV++GL L++   C+ALLR N++RY KNGLPPGTMGWP+FGET+EFLKQGPNFM +
Sbjct: 1   MGAMMVMMGLLLIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLKQGPNFMRN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           +R RYGSFFKSH+LG PT++SMD EVNRYIL NESKGLV GYPQSMLDILG CN+AAVHG
Sbjct: 61  QRLRYGSFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHG 120

Query: 121 DAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLK 180
            +H+LMRG+LL+++S TM++D +L +VD FMRS+L  W+  ++IDIQ+KTK MAF +SL 
Sbjct: 121 SSHRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLT 180

Query: 181 QTTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGN 240
           Q       P  E F   FFKLV+GT S+PIDLPGTNYR G+QARNNI +LLR+L+ ER +
Sbjct: 181 QIAGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRD 240

Query: 241 SKIQKDDMLGYLLSEE-NKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLGYL+ +E N+Y L +E+I DQV+T+ YSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQLREEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNG 360
           Q+LR EHLA RE+K   +P+  +D K+M+FTRAVI+ETSRLAT+VNGVLRKTT+DLE+NG
Sbjct: 301 QELRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEING 360

Query: 361 FLIPKGWRIY-------------------------DKSLESHNYFFLFGGGIRLCPGKEL 420
           +LIPKGWRIY                          KSLES N  F+FGGG RLCPGKEL
Sbjct: 361 YLIPKGWRIYVYTREINYDANLYEDPLIFNPWRWMKKSLESQNSCFVFGGGTRLCPGKEL 420

Query: 421 GIAEVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 438
           GI E+S+FLHYFVT+YRWEE+G  +L+ FPRV A  GFH+R+S Y
Sbjct: 421 GIVEISSFLHYFVTRYRWEEIGGDELMVFPRVFAPKGFHLRISPY 465

BLAST of CsaV3_4G028870 vs. TAIR 10
Match: AT5G38970.3 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 502.7 bits (1293), Expect = 3.0e-142
Identity = 246/370 (66.49%), Postives = 305/370 (82.43%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLL-CFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           MG  MV++GL L++   C+ALLR N++RY KNGLPPGTMGWP+FGET+EFLKQGPNFM +
Sbjct: 1   MGAMMVMMGLLLIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLKQGPNFMRN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           +R RYGSFFKSH+LG PT++SMD EVNRYIL NESKGLV GYPQSMLDILG CN+AAVHG
Sbjct: 61  QRLRYGSFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHG 120

Query: 121 DAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLK 180
            +H+LMRG+LL+++S TM++D +L +VD FMRS+L  W+  ++IDIQ+KTK MAF +SL 
Sbjct: 121 SSHRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLT 180

Query: 181 QTTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGN 240
           Q       P  E F   FFKLV+GT S+PIDLPGTNYR G+QARNNI +LLR+L+ ER +
Sbjct: 181 QIAGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRD 240

Query: 241 SKIQKDDMLGYLLSEE-NKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLGYL+ +E N+Y L +E+I DQV+T+ YSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQLREEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNG 360
           Q+LR EHLA RE+K   +P+  +D K+M+FTRAVI+ETSRLAT+VNGVLRKTT+DLE+NG
Sbjct: 301 QELRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEING 360

Query: 361 FLIPKGWRIY 368
           +LIPKGWRIY
Sbjct: 361 YLIPKGWRIY 370

BLAST of CsaV3_4G028870 vs. TAIR 10
Match: AT5G38970.2 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 476.1 bits (1224), Expect = 3.0e-134
Identity = 238/384 (61.98%), Postives = 292/384 (76.04%), Query Frame = 0

Query: 81  MDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGDAHKLMRGALLAIVSPTMIKD 140
           MD EVNRYIL NESKGLV GYPQSMLDILG CN+AAVHG +H+LMRG+LL+++S TM++D
Sbjct: 1   MDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHGSSHRLMRGSLLSLISSTMMRD 60

Query: 141 RLLLQVDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLKQTTSIESGPLWESFTIEFFKL 200
            +L +VD FMRS+L  W+  ++IDIQ+KTK MAF +SL Q       P  E F   FFKL
Sbjct: 61  HILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLTQIAGNLRKPFVEEFKTAFFKL 120

Query: 201 VLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSKIQKDDMLGYLLSEE-NKYK 260
           V+GT S+PIDLPGTNYR G+QARNNI +LLR+L+ ER +S     DMLGYL+ +E N+Y 
Sbjct: 121 VVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRDSGETFTDMLGYLMKKEGNRYP 180

Query: 261 LNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQLREEHLALREKKNPKDPID 320
           L +E+I DQV+T+ YSGYETVSTTSMMA+K+LHDHPK LQ+LR EHLA RE+K   +P+ 
Sbjct: 181 LTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKALQELRAEHLAFRERKRQDEPLG 240

Query: 321 WDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLIPKGWRIY----------- 380
            +D K+M+FTRAVI+ETSRLAT+VNGVLRKTT+DLE+NG+LIPKGWRIY           
Sbjct: 241 LEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEINGYLIPKGWRIYVYTREINYDAN 300

Query: 381 --------------DKSLESHNYFFLFGGGIRLCPGKELGIAEVSTFLHYFVTKYRWEEV 438
                          KSLES N  F+FGGG RLCPGKELGI E+S+FLHYFVT+YRWEE+
Sbjct: 301 LYEDPLIFNPWRWMKKSLESQNSCFVFGGGTRLCPGKELGIVEISSFLHYFVTRYRWEEI 360

BLAST of CsaV3_4G028870 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 298.1 bits (762), Expect = 1.1e-80
Identity = 164/468 (35.04%), Postives = 257/468 (54.91%), Query Frame = 0

Query: 5   MVIVGLFLLLCFCAA--LLRRNEVRYIKNGLPPGTMGWPVFGETSEFL----KQGPN-FM 64
           M      LLL   AA  LL     RY + GLPPG++G P+ GET + +     + P  F+
Sbjct: 1   MAFTAFLLLLSSIAAGFLLLLRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFI 60

Query: 65  NSKRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAV 124
           + + ARYGS F +H+ G PT+ S DPE NR++L NE K     YP S+ ++LGK ++  +
Sbjct: 61  DERVARYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLM 120

Query: 125 HGDAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSL 184
            G  HK M    ++  + ++IKD L+L +D  +R +L +W S+++ + E+ K++ F  ++
Sbjct: 121 KGSLHKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSWSSRVL-LMEEAKKITFELTV 180

Query: 185 KQTTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERG 244
           KQ  S + G   ES   E+  ++ G FSLP+ L  T YR  +QAR  + + L  ++ +R 
Sbjct: 181 KQLMSFDPGEWSESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRR 240

Query: 245 NSKIQ----KDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDH 304
             + +    K DML  LL+ ++ +  ++E+I+D ++ +  +GYET ST   +AVKFL + 
Sbjct: 241 EEEEEGAERKKDMLAALLAADDGF--SDEEIVDFLVALLVAGYETTSTIMTLAVKFLTET 300

Query: 305 PKVLQQLREEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDL 364
           P  L QL+EEH  +R  K+    ++W D+K+M FT+ V+ ET R+A ++ GV R+   D+
Sbjct: 301 PLALAQLKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDV 360

Query: 365 EVNGFLIPKGWRIYDKSLESH---------------------------NYFFLFGGGIRL 424
           E+ G+ IPKGW+++      H                           N F  FGGG RL
Sbjct: 361 EIKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFTPFGGGPRL 420

Query: 425 CPGKELGIAEVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRV 435
           CPG EL    +S FLH  VT + W    + KL+ FP  + +  + I V
Sbjct: 421 CPGYELARVALSVFLHRLVTGFSWVPAEQDKLVFFPTTRTQKRYPIFV 465

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAE8649648.14.0e-253100.00hypothetical protein Csa_012605 [Cucumis sativus][more]
XP_004150514.36.0e-24994.59cytochrome P450 85A [Cucumis sativus][more]
XP_008455791.17.6e-23689.39PREDICTED: cytochrome P450 85A-like [Cucumis melo][more]
XP_038890036.13.3e-21581.60cytochrome P450 85A-like [Benincasa hispida][more]
KAA0040382.12.6e-21287.85cytochrome P450 85A-like [Cucumis melo var. makuwa] >TYK04049.1 cytochrome P450 ... [more]
Match NameE-valueIdentityDescription
Q69F951.5e-17867.03Cytochrome P450 85A OS=Phaseolus vulgaris OX=3885 GN=BA13 PE=3 SV=2[more]
Q431474.5e-17565.09Cytochrome P450 85A1 OS=Solanum lycopersicum OX=4081 GN=CYP85A1 PE=2 SV=1[more]
Q940V43.8e-17464.73Cytochrome P450 85A2 OS=Arabidopsis thaliana OX=3702 GN=CYP85A2 PE=2 SV=1[more]
Q9FMA53.7e-16963.23Cytochrome P450 85A1 OS=Arabidopsis thaliana OX=3702 GN=CYP85A1 PE=2 SV=1[more]
Q50LE01.9e-15758.89Cytochrome P450 85A3 OS=Solanum lycopersicum OX=4081 GN=CYP85A3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L0Q53.0e-24694.32Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G410870 PE=3 SV=1[more]
A0A1S3C1A83.7e-23689.39cytochrome P450 85A-like OS=Cucumis melo OX=3656 GN=LOC103495886 PE=3 SV=1[more]
A0A5A7TA971.3e-21287.85Cytochrome P450 85A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A0A0KXL92.6e-21079.70Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G641590 PE=3 SV=1[more]
A0A5A7TDB41.7e-20979.48Cytochrome P450 85A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
Match NameE-valueIdentityDescription
AT3G30180.12.7e-17564.73brassinosteroid-6-oxidase 2 [more]
AT5G38970.12.6e-17063.23brassinosteroid-6-oxidase 1 [more]
AT5G38970.33.0e-14266.49brassinosteroid-6-oxidase 1 [more]
AT5G38970.23.0e-13461.98brassinosteroid-6-oxidase 1 [more]
AT5G05690.11.1e-8035.04Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 55..78
score: 22.28
coord: 371..387
score: 27.38
coord: 387..405
score: 24.9
IPR001128Cytochrome P450PRINTSPR00385P450coord: 330..341
score: 41.2
coord: 387..398
score: 32.17
coord: 378..387
score: 47.0
coord: 274..291
score: 29.56
IPR001128Cytochrome P450PFAMPF00067p450coord: 34..366
e-value: 2.6E-33
score: 115.5
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 24..436
e-value: 5.0E-78
score: 264.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 27..434
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 5..372
coord: 373..435
NoneNo IPR availablePANTHERPTHR24286:SF273CYTOCHROME P450 FAMILY PROTEINcoord: 5..372
coord: 373..435
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 380..389

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_4G028870.1CsaV3_4G028870.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen