CsaV3_4G017600 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_4G017600
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionRING-type E3 ubiquitin transferase
Locationchr4: 11006948 .. 11008035 (-)
RNA-Seq ExpressionCsaV3_4G017600
SyntenyCsaV3_4G017600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGAGTTAATTGCAACCAGATATTGGTGTCACATGTGTTCTCAAATGGTGAATCCCATCGTTGACGTGGAAATTAAATGCCCCTTCTGCCGCAGCGGCTTTGTTGAGGAGATAGGAAATGGTATTTCCAGCGACAATAACGATAACAACAACAATAACAATAATAACAACGAGCTCGAATCCGATTTTGGGTCGGAGCGAGCTTTGTCTCTCTGGGCTCCGATCTTGCTGGGTATGATGGGAAATCCCCGCGGGCGACGAAGATTCAGACATCTAGAGTTTGACGACGACGATGATGATAATGAGCATGAGGATGGCGAGGGGAATCGTGTGAGTAGTGAGACGACTGAGCTTGATTCAGTCATTAGGAGGAGGAGAAGTTCAGCTACCATTCTTCAGTTGCTCCAAGGTATCAGGGCTGGAATTGCAACTGCTGAATCAGAGAATAGTTATGAGGAGAGAGGAGCAGAGAAAGAGAGAGGGTTATTCTTATAAATCCCTTCAATCAGACCATTGTAGTTCAAGGAGGTGAGAATCAAAATCAAAACCAAAACTCCATTGGGTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATATAGCCGAGAATGATCCCAACAGATATGGCACTCCACCAGCACAGAAGGAGGCAGTTGATGCGCTGCCCACCGTGAGGGTTGAACTTGAGGAGGATAGCTGTTTGCAATGTTCAGTGTGTTTGGATGAGTTTGAGGTGGATGAAGAAGCCAAGGAGATGCCATGTAAGCATAAATTTCATACAGGTTGTATTCTGCCATGGTTGGAGCTGCATAGTTCTTGCCCAGTTTGTAGGCATCAATTGCCTGGGGATGAATCCAAGCGTGATGTTGATGGTGGGTCTGCTGCGAGGTTCATGAGCGACCTCAATAATGGTAATGGCAATGGCAGCGGGGAGATTGAGGGGAGAAATTCATCTGAGTCTGGGAGGAGGTTTTCGTTTCCATGGCCTTTTAATGGCTTGTTTACTTCCCAAGGAAACTCCTCGGCTGGAAGTGCAAGTGATTCTCAAAGGGATCACACTTCCATTTGA

mRNA sequence

ATGGACGAGTTAATTGCAACCAGATATTGGTGTCACATGTGTTCTCAAATGGTGAATCCCATCGTTGACGTGGAAATTAAATGCCCCTTCTGCCGCAGCGGCTTTGTTGAGGAGATAGGAAATGGTATTTCCAGCGACAATAACGATAACAACAACAATAACAATAATAACAACGAGCTCGAATCCGATTTTGGGTCGGAGCGAGCTTTGTCTCTCTGGGCTCCGATCTTGCTGGGTATGATGGGAAATCCCCGCGGGCGACGAAGATTCAGACATCTAGAGTTTGACGACGACGATGATGATAATGAGCATGAGGATGGCGAGGGGAATCGTGTGAGTAGTGAGACGACTGAGCTTGATTCAGTCATTAGGAGGAGGAGAAGTTCAGCTACCATTCTTCAGTTGCTCCAAGTTCAAGGAGGTGAGAATCAAAATCAAAACCAAAACTCCATTGGGTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATATAGCCGAGAATGATCCCAACAGATATGGCACTCCACCAGCACAGAAGGAGGCAGTTGATGCGCTGCCCACCGTGAGGGTTGAACTTGAGGAGGATAGCTGTTTGCAATGTTCAGTGTGTTTGGATGAGTTTGAGGTGGATGAAGAAGCCAAGGAGATGCCATGTAAGCATAAATTTCATACAGGTTGTATTCTGCCATGGTTGGAGCTGCATAGTTCTTGCCCAGTTTGTAGGCATCAATTGCCTGGGGATGAATCCAAGCGTGATGTTGATGGTGGGTCTGCTGCGAGGTTCATGAGCGACCTCAATAATGGTAATGGCAATGGCAGCGGGGAGATTGAGGGGAGAAATTCATCTGAGTCTGGGAGGAGGTTTTCGTTTCCATGGCCTTTTAATGGCTTGTTTACTTCCCAAGGAAACTCCTCGGCTGGAAGTGCAAGTGATTCTCAAAGGGATCACACTTCCATTTGA

Coding sequence (CDS)

ATGGACGAGTTAATTGCAACCAGATATTGGTGTCACATGTGTTCTCAAATGGTGAATCCCATCGTTGACGTGGAAATTAAATGCCCCTTCTGCCGCAGCGGCTTTGTTGAGGAGATAGGAAATGGTATTTCCAGCGACAATAACGATAACAACAACAATAACAATAATAACAACGAGCTCGAATCCGATTTTGGGTCGGAGCGAGCTTTGTCTCTCTGGGCTCCGATCTTGCTGGGTATGATGGGAAATCCCCGCGGGCGACGAAGATTCAGACATCTAGAGTTTGACGACGACGATGATGATAATGAGCATGAGGATGGCGAGGGGAATCGTGTGAGTAGTGAGACGACTGAGCTTGATTCAGTCATTAGGAGGAGGAGAAGTTCAGCTACCATTCTTCAGTTGCTCCAAGTTCAAGGAGGTGAGAATCAAAATCAAAACCAAAACTCCATTGGGTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATATAGCCGAGAATGATCCCAACAGATATGGCACTCCACCAGCACAGAAGGAGGCAGTTGATGCGCTGCCCACCGTGAGGGTTGAACTTGAGGAGGATAGCTGTTTGCAATGTTCAGTGTGTTTGGATGAGTTTGAGGTGGATGAAGAAGCCAAGGAGATGCCATGTAAGCATAAATTTCATACAGGTTGTATTCTGCCATGGTTGGAGCTGCATAGTTCTTGCCCAGTTTGTAGGCATCAATTGCCTGGGGATGAATCCAAGCGTGATGTTGATGGTGGGTCTGCTGCGAGGTTCATGAGCGACCTCAATAATGGTAATGGCAATGGCAGCGGGGAGATTGAGGGGAGAAATTCATCTGAGTCTGGGAGGAGGTTTTCGTTTCCATGGCCTTTTAATGGCTTGTTTACTTCCCAAGGAAACTCCTCGGCTGGAAGTGCAAGTGATTCTCAAAGGGATCACACTTCCATTTGA

Protein sequence

MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNELESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELDSVIRRRRSSATILQLLQVQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHTSI*
Homology
BLAST of CsaV3_4G017600 vs. NCBI nr
Match: KAE8649424.1 (hypothetical protein Csa_021650 [Cucumis sativus])

HSP 1 Score: 660.6 bits (1703), Expect = 7.0e-186
Identity = 325/325 (100.00%), Postives = 325/325 (100.00%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQVQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGT 180
           SVIRRRRSSATILQLLQVQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGT
Sbjct: 121 SVIRRRRSSATILQLLQVQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGT 180

Query: 181 PPAQKEAVDALPTVRVELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELH 240
           PPAQKEAVDALPTVRVELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELH
Sbjct: 181 PPAQKEAVDALPTVRVELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELH 240

Query: 241 SSCPVCRHQLPGDESKRDVDGGSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPF 300
           SSCPVCRHQLPGDESKRDVDGGSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPF
Sbjct: 241 SSCPVCRHQLPGDESKRDVDGGSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPF 300

Query: 301 NGLFTSQGNSSAGSASDSQRDHTSI 326
           NGLFTSQGNSSAGSASDSQRDHTSI
Sbjct: 301 NGLFTSQGNSSAGSASDSQRDHTSI 325

BLAST of CsaV3_4G017600 vs. NCBI nr
Match: XP_031739817.1 (LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SIRP1 [Cucumis sativus])

HSP 1 Score: 642.5 bits (1656), Expect = 2.0e-180
Identity = 325/361 (90.03%), Postives = 325/361 (90.03%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQ------------------------------------VQGGENQ 180
           SVIRRRRSSATILQLLQ                                    VQGGENQ
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEERSRERERVILINPFNQTIVVQGGENQ 180

Query: 181 NQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSCL 240
           NQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSCL
Sbjct: 181 NQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSCL 240

Query: 241 QCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSA 300
           QCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSA
Sbjct: 241 QCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSA 300

Query: 301 ARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHTS 326
           ARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHTS
Sbjct: 301 ARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHTS 360

BLAST of CsaV3_4G017600 vs. NCBI nr
Match: XP_008449904.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008449905.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0040122.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK21744.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 624.8 bits (1610), Expect = 4.2e-175
Identity = 321/366 (87.70%), Postives = 321/366 (87.70%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGN VSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQ-------------------------------------VQGGEN 180
           SVIRRRRSSATILQLLQ                                     VQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180

Query: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC 240
           QNQN NSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDS 
Sbjct: 181 QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS
Sbjct: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300

Query: 301 AARFMSDLN----NGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 326
           AARFMSDLN    NGNGNGSGEIE RNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ
Sbjct: 301 AARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360

BLAST of CsaV3_4G017600 vs. NCBI nr
Match: XP_038901761.1 (E3 ubiquitin-protein ligase SIRP1 [Benincasa hispida] >XP_038901762.1 E3 ubiquitin-protein ligase SIRP1 [Benincasa hispida])

HSP 1 Score: 566.6 bits (1459), Expect = 1.4e-157
Identity = 295/363 (81.27%), Postives = 307/363 (84.57%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGIS   NDNN+NN+NNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGIS---NDNNDNNSNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDDDNEHEDGEGNRVSSE T+LD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNEHEDGEGNRVSSEPTDLD 120

Query: 121 SVIRRRRSSATILQLLQ-------------------------------------VQGGEN 180
           SVIRRRRSSATILQLLQ                                     VQGGE 
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGMATAESENSYEGERSRERERVILINPFNQTIVVQGGE- 180

Query: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC 240
            NQNQN IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVDALPTVRVE   DSC
Sbjct: 181 -NQNQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVE-PVDSC 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQC+VCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRD DGGS
Sbjct: 241 LQCAVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDADGGS 300

Query: 301 AARFMSDLNNGNGNGSGE-IEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDH 326
             RFM DLNN NG+GSGE I+GRN+S +GRRFSFPWPFNGLF++QGNSS GSASDSQRDH
Sbjct: 301 -TRFMGDLNNANGSGSGEIIDGRNASGTGRRFSFPWPFNGLFSAQGNSSGGSASDSQRDH 356

BLAST of CsaV3_4G017600 vs. NCBI nr
Match: XP_022944289.1 (E3 ubiquitin-protein ligase SIRP1-like [Cucurbita moschata])

HSP 1 Score: 534.3 bits (1375), Expect = 7.5e-148
Identity = 284/374 (75.94%), Postives = 303/374 (81.02%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDE IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEE+GNG+SSDNN NNNNNN+NN L
Sbjct: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDN---EHEDGEGNRVSSETT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDDDN   EH DGEGNRVSSE T
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120

Query: 121 ELDSVI-RRRRSSATILQLLQ--------------------------------------- 180
           ELDSVI RRRRSSATILQLLQ                                       
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180

Query: 181 ------VQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDAL 240
                 VQGGE  +QN N+IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVDAL
Sbjct: 181 FNQTIVVQGGE--SQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240

Query: 241 PTVRVELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLP 300
           PTVRVE   D+ LQCSVCLDEFE+DEEAKEMPC+HKFH+GCILPWLELHSSCPVCRHQLP
Sbjct: 241 PTVRVEC-GDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLP 300

Query: 301 GDESKRDVDGGSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSS 326
            DESKRD DGGS ARFM    +G+G+GSGEI+GRN+S +GRRFSFPWPFNGLF+SQGNSS
Sbjct: 301 ADESKRDADGGS-ARFMGS-GSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSS 360

BLAST of CsaV3_4G017600 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.6e-47
Identity = 135/349 (38.68%), Postives = 180/349 (51.58%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           M E   TRYWCH C Q +   +  EIKCP C  GFVEE+         D       N + 
Sbjct: 1   MAEAAITRYWCHECEQAIEEAMVDEIKCPSCGGGFVEEM--------TDEEIERLTNRQP 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNE-HEDGEG-----NRVSS 120
           E  F      S W PI                   D DD+DN+   + EG      R S+
Sbjct: 61  EPGF------SQWNPI------------EHPGETMDSDDEDNDLGREFEGFIRRHRRAST 120

Query: 121 ETTELDSV-----IRRRRSSATIL----QLLQVQGG--------ENQNQNQNSIGSLGDY 180
               LDS+       + R S+ ++    Q L +QG          +Q  + N  G L +Y
Sbjct: 121 LRRVLDSIHDDLADDQERDSSILINAFNQALALQGSVLDPDEGQGDQGGSTNDDGLLEEY 180

Query: 181 FVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSCLQCSVCLDEFEVDE 240
            +G GL LLLQH+AE+DP+R GTPPA+KEAV+ALPTV++E      + CSVCLD+ EV  
Sbjct: 181 VLGAGLSLLLQHLAESDPSRNGTPPAKKEAVEALPTVKIE----EVVSCSVCLDDLEVGS 240

Query: 241 EAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSAARFMSDLNNGNGN 300
           +AK+MPC+HKFH+ CILPWLELHSSCPVCR +LP +E+K   +  +  R          +
Sbjct: 241 QAKQMPCEHKFHSSCILPWLELHSSCPVCRFELPSEETKDLNEPSNIGRVEDSHEEVRAD 300

Query: 301 GSGEIEGRNSSESGRRFSF-PWPFNGLF-TSQGNSSAGSASDSQRDHTS 325
           G G +    S  S R ++  PW  N LF T +  ++ G ++D Q  HTS
Sbjct: 301 GPGNV----SESSNRPWAIVPW-LNELFSTREAQNAGGVSTDQQSPHTS 314

BLAST of CsaV3_4G017600 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 2.2e-41
Identity = 116/315 (36.83%), Postives = 160/315 (50.79%), Query Frame = 0

Query: 9   YWCHMCSQMVNPIV--DVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNELESDFGS 68
           ++C+ C+Q V   +    +  CP C  GF+EE       D N N + N N N  +S F  
Sbjct: 21  FFCYQCNQTVTISISSSADPFCPICNQGFLEEY-----EDPNPNQSLNFNPNSSDSFFPM 80

Query: 69  ERALSLWAPILLG-MMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELDSVIRR 128
               S   P++ G     P G                  +    +      T L + ++ 
Sbjct: 81  ADPFSTLLPLIFGSSAAAPSGMDFMSLFGPSMQPQARSTQQNPQSDAFDPFTFLQNHLQT 140

Query: 129 RRSSATILQLLQVQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQK 188
            RSS T  +   V      +      G+ GDYF GPGL+ L+Q +AENDPNRYGTPPA K
Sbjct: 141 LRSSGTHFEF--VIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASK 200

Query: 189 EAVDALPTVRV--ELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSC 248
            A+DALPTV+V  ++ +    QC+VC+DEFE   + K+MPCKH FH  C+LPWLELH+SC
Sbjct: 201 SAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSC 260

Query: 249 PVCRHQLPGDESKRDVDGGSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFS--FPWPFN 308
           PVCR +LP D+   D +  S        + G+G+G G +EG+ +     RFS   PWPF 
Sbjct: 261 PVCRFELPTDDP--DYENRSQG------SQGSGDGQGSVEGQQTP----RFSIQLPWPFR 312

Query: 309 GLFTSQGNSSAGSAS 317
                Q  S +GS +
Sbjct: 321 ----RQDGSGSGSGA 312

BLAST of CsaV3_4G017600 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.3e-37
Identity = 111/326 (34.05%), Postives = 166/326 (50.92%), Query Frame = 0

Query: 9   YWCHMCSQMVNPIV---DVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNELESDFG 68
           ++C+ C++ VN  +     +  CP C  GF+EE  N   +  +   N N N+      F 
Sbjct: 23  FFCYQCNRTVNVTISPPSSDPTCPICNEGFLEEYDNPNPNQGSGFLNPNPNSIPFHDLF- 82

Query: 69  SERALSLWAPI-----LLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSET-TEL 128
               L+L  P      LL    +         ++ ++         G G+  + +  T +
Sbjct: 83  ----LTLSDPFASLLPLLFPSSSSTTTSSSASIDPNNPSLSGPTRSGRGDPFAFDPFTFI 142

Query: 129 DSVIRRRRSSATILQLLQVQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYG 188
            + +   RSS   ++ + +Q   + +Q      ++GDYF+GPGL+ L+Q +AENDPNRYG
Sbjct: 143 QNHLNDLRSSGAQIEFV-IQNNPS-DQGFRLPANIGDYFIGPGLEQLIQQLAENDPNRYG 202

Query: 189 TPPAQKEAVDALPTVRVELE--EDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWL 248
           TPPA K A++ALP V +          QC+VC+D+FE   EAK+MPCKH +H  C+LPWL
Sbjct: 203 TPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWL 262

Query: 249 ELHSSCPVCRHQLPGDES--KRDVDGGSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFS 308
           ELH+SCPVCRH+LP D+   +R V G   A+  S  N+G+ +G        + E   R S
Sbjct: 263 ELHNSCPVCRHELPTDDPDYERRVRG---AQGTSGGNDGDNSGQRSDGDNRTVERSFRIS 322

Query: 309 FPWPFNGLFTSQGNSSAGSASDSQRD 322
            PWPF       G +   +A   Q D
Sbjct: 323 LPWPFQA--RGPGPAPGDNAETRQED 336

BLAST of CsaV3_4G017600 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 6.4e-25
Identity = 100/330 (30.30%), Postives = 145/330 (43.94%), Query Frame = 0

Query: 5   IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNELESDF 64
           I   +WCH C + V  +   +  C +C  GFVEEI  G S  + D         E +  F
Sbjct: 4   IRNTHWCHRCQRAV-WLRARDAVCSYCGGGFVEEIDIGPSRAHRD--------VERDPTF 63

Query: 65  GSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELDSVIR 124
               A S                  F      +   D E     G+  S   + L  ++ 
Sbjct: 64  DLMEAFS-----------------AFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLI 123

Query: 125 RRRSSATILQLLQVQGGENQ------NQNQNSIG-----SLGDYFVGPGLDLLLQHIAEN 184
               +       ++ GG+N       N     IG     + GDYF GPGL+ L++ ++  
Sbjct: 124 FGGQAP-----FRLAGGDNSSVEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSG 183

Query: 185 DPNRYGTPPAQKEAVDALPTVRVELE--EDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTG 244
             +R G PPA K ++DALPT+++  +  + S   C VC DEFE+  EAK+MPC H +H+ 
Sbjct: 184 THHR-GPPPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSD 243

Query: 245 CILPWLELHSSCPVCRHQLPGDESKRDVDGGSAARFMSDLNNGNGNGSGEIEGRNSSESG 304
           CI+PWL  H+SCPVCR +LP          GS++   S  N           GR +S   
Sbjct: 244 CIVPWLVQHNSCPVCRKELP--------SRGSSSSTQSSQNRST-------NGRENSRRR 278

Query: 305 RRFSFPWPFNGLFTSQGNSSAGSASDSQRD 322
             FS  WPF         SS+ S++ ++RD
Sbjct: 304 NIFSNLWPF--------RSSSSSSTQNRRD 278

BLAST of CsaV3_4G017600 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.5e-21
Identity = 94/310 (30.32%), Postives = 135/310 (43.55%), Query Frame = 0

Query: 9   YWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNELESDFGSER 68
           +WCH C + V  +   E  C +C  GFVEE+    +S   D   ++    E +  F    
Sbjct: 8   HWCHRCQRAVR-LHGQEPVCFYCGGGFVEELDMAQASP-FDMFRSHRGVVERDQTFDLMD 67

Query: 69  ALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVS-SETTELDSVIRRRR 128
           A S++             R R      D +        G  N    +        +  R 
Sbjct: 68  AFSVFM------------RNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRL 127

Query: 129 SSATILQLLQVQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEA 188
           +    ++ L   G       +   G+ GDYF GPGL+ L + ++     R G PPA + A
Sbjct: 128 TGDNAVEALFNGGSPGIGITR---GNTGDYFFGPGLEELFEQLSAGTTRR-GPPPAPRSA 187

Query: 189 VDALPTVRVELE--EDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPV 248
           +DALPT+++       S   C VC DEFE+  EAK+MPC H +H+ CI+PWL  H+SCPV
Sbjct: 188 IDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPV 247

Query: 249 CRHQLPGDESKRDVDGGSAARFMSDLNNGNGNGSGEIEGR-NSSESGRRFSFPWPFNGLF 308
           CR +LP           S  R     N  + + S     R N +E    FS  WPF    
Sbjct: 248 CRQELPSASG----PSSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWPFR--- 291

Query: 309 TSQGNSSAGS 315
            S G+SS+ +
Sbjct: 308 -SSGSSSSST 291

BLAST of CsaV3_4G017600 vs. ExPASy TrEMBL
Match: A0A0A0KWR3 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G267990 PE=4 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 1.2e-180
Identity = 325/362 (89.78%), Postives = 325/362 (89.78%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQ-------------------------------------VQGGEN 180
           SVIRRRRSSATILQLLQ                                     VQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180

Query: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC 240
           QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC
Sbjct: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS
Sbjct: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300

Query: 301 AARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHT 326
           AARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHT
Sbjct: 301 AARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHT 360

BLAST of CsaV3_4G017600 vs. ExPASy TrEMBL
Match: A0A1S3BNR7 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491641 PE=4 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.1e-175
Identity = 321/366 (87.70%), Postives = 321/366 (87.70%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGN VSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQ-------------------------------------VQGGEN 180
           SVIRRRRSSATILQLLQ                                     VQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180

Query: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC 240
           QNQN NSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDS 
Sbjct: 181 QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS
Sbjct: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300

Query: 301 AARFMSDLN----NGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 326
           AARFMSDLN    NGNGNGSGEIE RNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ
Sbjct: 301 AARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360

BLAST of CsaV3_4G017600 vs. ExPASy TrEMBL
Match: A0A5D3DDU3 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G001160 PE=4 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.1e-175
Identity = 321/366 (87.70%), Postives = 321/366 (87.70%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGN VSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQ-------------------------------------VQGGEN 180
           SVIRRRRSSATILQLLQ                                     VQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180

Query: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC 240
           QNQN NSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDS 
Sbjct: 181 QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS
Sbjct: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300

Query: 301 AARFMSDLN----NGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 326
           AARFMSDLN    NGNGNGSGEIE RNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ
Sbjct: 301 AARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360

BLAST of CsaV3_4G017600 vs. ExPASy TrEMBL
Match: A0A6J1FWF9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448781 PE=4 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 3.6e-148
Identity = 284/374 (75.94%), Postives = 303/374 (81.02%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDE IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEE+GNG+SSDNN NNNNNN+NN L
Sbjct: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDN---EHEDGEGNRVSSETT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDDDN   EH DGEGNRVSSE T
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120

Query: 121 ELDSVI-RRRRSSATILQLLQ--------------------------------------- 180
           ELDSVI RRRRSSATILQLLQ                                       
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180

Query: 181 ------VQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDAL 240
                 VQGGE  +QN N+IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVDAL
Sbjct: 181 FNQTIVVQGGE--SQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240

Query: 241 PTVRVELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLP 300
           PTVRVE   D+ LQCSVCLDEFE+DEEAKEMPC+HKFH+GCILPWLELHSSCPVCRHQLP
Sbjct: 241 PTVRVEC-GDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLP 300

Query: 301 GDESKRDVDGGSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSS 326
            DESKRD DGGS ARFM    +G+G+GSGEI+GRN+S +GRRFSFPWPFNGLF+SQGNSS
Sbjct: 301 ADESKRDADGGS-ARFMGS-GSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSS 360

BLAST of CsaV3_4G017600 vs. ExPASy TrEMBL
Match: A0A6J1JDY5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483664 PE=4 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 1.9e-144
Identity = 281/380 (73.95%), Postives = 300/380 (78.95%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDE IATRYWCH+CSQMVNPIVDVEIKCPFCRSGFVEE+GNG+SSDNN  NNNNN+NN L
Sbjct: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNN-GNNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDN---EHEDGEGNRVSSETT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDD+N   EH DGEGNR SSE  
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPA 120

Query: 121 ELDSVI-RRRRSSATILQLLQ--------------------------------------- 180
           ELDSVI RRRRSSATILQLLQ                                       
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILI 180

Query: 181 --------VQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVD 240
                   VQGGE  +QN N+IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVD
Sbjct: 181 NPFNQTIVVQGGE--SQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVD 240

Query: 241 ALPTVRVELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQ 300
           ALPTVRVE   D+ LQCSVCLDEFE+DEEAKEMPCKHKFH+GCILPWLELHSSCPVCRHQ
Sbjct: 241 ALPTVRVEC-GDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQ 300

Query: 301 LPGDESKRDVDGGSAARFMSDLNNGN----GNGSGEIEGRNSSESGRRFSFPWPFNGLFT 326
           LP DESKRD DGGS ARFM +L+NGN    G+GSGEI+GR +S  GRRFSFPWPFNGLF+
Sbjct: 301 LPADESKRDTDGGS-ARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFS 360

BLAST of CsaV3_4G017600 vs. TAIR 10
Match: AT5G56340.1 (RING/U-box superfamily protein )

HSP 1 Score: 322.8 bits (826), Expect = 3.2e-88
Identity = 193/390 (49.49%), Postives = 239/390 (61.28%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEI-GNGISSDNNDNNNNNNNNNE 60
           M+E +A RYWCHMCSQMVNP+++ EIKCPFC+SGF+EE+ GNG             +  +
Sbjct: 1   MEETMAARYWCHMCSQMVNPVMESEIKCPFCQSGFIEEMSGNG----GGGGGRGIRDVQD 60

Query: 61  LESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDN-----EHEDGEGNRVSS 120
            E+DFG++RALSLWAPILLGMM +PR RRRFR  EF +++DDN      + DG GN  ++
Sbjct: 61  SETDFGTDRALSLWAPILLGMMSSPRRRRRFRRSEFGEENDDNGDDLTTNADGNGNDSNN 120

Query: 121 ETT-------------------ELDSVI-RRRRSSATILQLLQ-VQGG------------ 180
                                 E +S++ RRRRSS  ILQLLQ ++ G            
Sbjct: 121 SNNNVYRHHRARRHGGEIDLDREFESILRRRRRSSGNILQLLQGIRAGIASEYESSDNNW 180

Query: 181 ----------------------ENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYG 240
                                  +QNQN  S+ SLGDYF+GPGLDLLLQH+AENDPNR G
Sbjct: 181 DNSRERDRVIMINPYNQSLVVPSDQNQNHPSLTSLGDYFIGPGLDLLLQHLAENDPNRQG 240

Query: 241 TPPAQKEAVDALPTVRVELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLEL 300
           TPPA+KEAV+ALPTV++       LQCSVCLD+FE   EAKEMPCKHKFH  CI+PWLEL
Sbjct: 241 TPPARKEAVEALPTVKIM----EPLQCSVCLDDFEKGTEAKEMPCKHKFHVRCIVPWLEL 300

Query: 301 HSSCPVCRHQLPG-----DESKRDVDGGSAARFMSDLNNGN-----GNGSGEIEGRNSSE 320
           HSSCPVCR +LP      DE+K D +     R + + +NGN     GN     E    S 
Sbjct: 301 HSSCPVCRFELPSSADDDDETKTDSERVLRTRNVRETSNGNVVENVGNADRGREDEVRSG 360

BLAST of CsaV3_4G017600 vs. TAIR 10
Match: AT4G26400.2 (RING/U-box superfamily protein )

HSP 1 Score: 301.2 bits (770), Expect = 1.0e-81
Identity = 177/366 (48.36%), Postives = 227/366 (62.02%), Query Frame = 0

Query: 5   IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNELESDF 64
           ++ RYWCHMCSQMVNP++  EIKCPFC+SGFVEE+   I   N  ++++     + E DF
Sbjct: 1   MSARYWCHMCSQMVNPVIGAEIKCPFCQSGFVEEMSREI---NGGSSSSLREVQDPEIDF 60

Query: 65  GSERALSLWAPILLGMMGNPRGRRRFRHLEFD-DDDDDNEHEDGEGN------------- 124
           G++RALSLW PILLGMM NPR RRRFR  EF  D+D+ N  +D +GN             
Sbjct: 61  GTDRALSLWGPILLGMMSNPRRRRRFRRTEFGVDNDEVNVSDDVDGNDSVDIDRHHHRRH 120

Query: 125 ------RVSSETTELDSVI-RRRRSSATILQLLQ-----------------------VQG 184
                 R      E +S++ RRRRSSATILQLLQ                       VQG
Sbjct: 121 RHRQQGREIDLDREFESILRRRRRSSATILQLLQGIRAGIASEYESSDRDLLNQSAVVQG 180

Query: 185 GE--NQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVEL 244
               NQN+N  S+ ++GDYFVG  LD LL+H+A+ND  R+G+ PA+KE VD LPTV++  
Sbjct: 181 STSLNQNRNNTSLSAIGDYFVGSSLDHLLEHLADNDSIRHGSLPARKEVVDNLPTVKI-- 240

Query: 245 EEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRD 304
                LQCS+CLD+F+   EAKEMPCKHKFH  CI+PWLELHSSCPVCR++LP D+  + 
Sbjct: 241 --SESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVPWLELHSSCPVCRYELPPDDETKV 300

Query: 305 VDGGSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDS 325
                  R + ++N  N N   E + RNS+ S RRFSFPWPF+GLF+S  +SSA ++  S
Sbjct: 301 NPVRPRTRTL-EINVSNEN--VEDDARNSNVSERRFSFPWPFSGLFSSSSSSSASASGSS 356

BLAST of CsaV3_4G017600 vs. TAIR 10
Match: AT4G26400.1 (RING/U-box superfamily protein )

HSP 1 Score: 301.2 bits (770), Expect = 1.0e-81
Identity = 177/366 (48.36%), Postives = 227/366 (62.02%), Query Frame = 0

Query: 5   IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNELESDF 64
           ++ RYWCHMCSQMVNP++  EIKCPFC+SGFVEE+   I   N  ++++     + E DF
Sbjct: 1   MSARYWCHMCSQMVNPVIGAEIKCPFCQSGFVEEMSREI---NGGSSSSLREVQDPEIDF 60

Query: 65  GSERALSLWAPILLGMMGNPRGRRRFRHLEFD-DDDDDNEHEDGEGN------------- 124
           G++RALSLW PILLGMM NPR RRRFR  EF  D+D+ N  +D +GN             
Sbjct: 61  GTDRALSLWGPILLGMMSNPRRRRRFRRTEFGVDNDEVNVSDDVDGNDSVDIDRHHHRRH 120

Query: 125 ------RVSSETTELDSVI-RRRRSSATILQLLQ-----------------------VQG 184
                 R      E +S++ RRRRSSATILQLLQ                       VQG
Sbjct: 121 RHRQQGREIDLDREFESILRRRRRSSATILQLLQGIRAGIASEYESSDRDLLNQSAVVQG 180

Query: 185 GE--NQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVEL 244
               NQN+N  S+ ++GDYFVG  LD LL+H+A+ND  R+G+ PA+KE VD LPTV++  
Sbjct: 181 STSLNQNRNNTSLSAIGDYFVGSSLDHLLEHLADNDSIRHGSLPARKEVVDNLPTVKI-- 240

Query: 245 EEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRD 304
                LQCS+CLD+F+   EAKEMPCKHKFH  CI+PWLELHSSCPVCR++LP D+  + 
Sbjct: 241 --SESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVPWLELHSSCPVCRYELPPDDETKV 300

Query: 305 VDGGSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDS 325
                  R + ++N  N N   E + RNS+ S RRFSFPWPF+GLF+S  +SSA ++  S
Sbjct: 301 NPVRPRTRTL-EINVSNEN--VEDDARNSNVSERRFSFPWPFSGLFSSSSSSSASASGSS 356

BLAST of CsaV3_4G017600 vs. TAIR 10
Match: AT1G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 273.5 bits (698), Expect = 2.2e-73
Identity = 168/370 (45.41%), Postives = 218/370 (58.92%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           M+E + TRYWCHMCSQ VNP+++ EIKCPFC+SGFVEE+ +    D++D  +   NN   
Sbjct: 1   MEEAVVTRYWCHMCSQTVNPVMEAEIKCPFCQSGFVEEMEDDDDHDSSDPADVRANN--- 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDD-----DNEHEDGEGNRVSSE 120
                     SLWAPIL+ +M +P  RRR + +E  +D+      +N  +DGE N +  +
Sbjct: 61  ----------SLWAPILMELMNDPVRRRRNQSVESVEDNQNEVQTENNEDDGE-NDLDWQ 120

Query: 121 TTELDSVIRRRRSSATILQLLQ-------------VQGGEN---------QNQN---QNS 180
             E+  + RRRR SA +LQLLQ               GG N          NQ    QNS
Sbjct: 121 LQEI--LRRRRRHSAAVLQLLQGIRAGLSVESESTGNGGNNPGRVILINTSNQTITVQNS 180

Query: 181 -------IGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC 240
                   GSLGDYF+GPG ++LLQ +AENDPNRYGTPPA+KEAV+AL TV++E      
Sbjct: 181 ADMDSVPAGSLGDYFIGPGFEMLLQRLAENDPNRYGTPPAKKEAVEALATVKIE----ET 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRD----- 300
           LQCSVCLD+FE+  EAK MPC HKFH+ C+LPWLELHSSCPVCR+QLP DE+K D     
Sbjct: 241 LQCSVCLDDFEIGTEAKLMPCTHKFHSDCLLPWLELHSSCPVCRYQLPADEAKTDSVTTT 300

Query: 301 VDGGSAARFMSDLNNGNGNGSG------------EIEGRNSSESGRRFSFPWPFNGLFTS 317
            D   ++   +  ++G  N  G            E E  N +  G  FS PWPF+ LF+S
Sbjct: 301 SDNNGSSSASATTSHGAENSDGNRRQEEEEEEEEEEEEENENNDGSGFSIPWPFSTLFSS 350

BLAST of CsaV3_4G017600 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 218.0 bits (554), Expect = 1.1e-56
Identity = 141/336 (41.96%), Postives = 185/336 (55.06%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVE-IKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNE 60
           M++   T YWCHMCS+ V P++  E IKC FC+SGFVEE+      DNND++        
Sbjct: 1   MEDASETSYWCHMCSRSVIPLIQDEIIKCNFCQSGFVEEM------DNNDDH-------- 60

Query: 61  LESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEF-----DDDDDDNEHEDGEGNRVS- 120
                  + A SLW PIL+ MM N   +      +      D+D+D+++ +DG+ N    
Sbjct: 61  -------QAADSLWTPILMEMMNNNHDQHSTNQEDSESILEDEDEDEDDGDDGDQNNDGE 120

Query: 121 -SETTELDSVIR-RRRSSATILQLLQ-------VQGGENQNQNQNS-------------- 180
              T +L+ + R R R S  I+ LLQ       ++   N++   NS              
Sbjct: 121 IDITHQLEEIRRIRTRHSTAIVNLLQGIRAGLLIESENNEDNPDNSELVVLINSFNQRIR 180

Query: 181 ------------IGSLGDYFVGPGLDLLLQHIAEND-PNRYGTPPAQKEAVDALPTVRVE 240
                        GSLGDYF+GPG + LLQ +AEND  NRYGTPPA KEAV+AL  V++ 
Sbjct: 181 VHQDSVDTTSVPSGSLGDYFIGPGFETLLQRLAENDLNNRYGTPPATKEAVEALAMVKI- 240

Query: 241 LEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLP---GDE 289
             EDS LQCSVCLD+FE+  EAKEMPCKHKFH+ C+LPWLELHSSCPVCR+ LP    DE
Sbjct: 241 --EDSLLQCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPWLELHSSCPVCRYLLPTGDDDE 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAE8649424.17.0e-186100.00hypothetical protein Csa_021650 [Cucumis sativus][more]
XP_031739817.12.0e-18090.03LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SIRP1 [Cucumis sativus][more]
XP_008449904.14.2e-17587.70PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008449905.1... [more]
XP_038901761.11.4e-15781.27E3 ubiquitin-protein ligase SIRP1 [Benincasa hispida] >XP_038901762.1 E3 ubiquit... [more]
XP_022944289.17.5e-14875.94E3 ubiquitin-protein ligase SIRP1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q6AVN24.6e-4738.68E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI... [more]
Q8LPN72.2e-4136.83E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
P0CH303.3e-3734.05E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
Q94AK46.4e-2530.30E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV... [more]
O221971.5e-2130.32Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Match NameE-valueIdentityDescription
A0A0A0KWR31.2e-18089.78RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G267990 PE... [more]
A0A1S3BNR72.1e-17587.70RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491641 PE=4 ... [more]
A0A5D3DDU32.1e-17587.70RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1FWF93.6e-14875.94RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448781... [more]
A0A6J1JDY51.9e-14473.95RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483664 P... [more]
Match NameE-valueIdentityDescription
AT5G56340.13.2e-8849.49RING/U-box superfamily protein [more]
AT4G26400.21.0e-8148.36RING/U-box superfamily protein [more]
AT4G26400.11.0e-8148.36RING/U-box superfamily protein [more]
AT1G55530.12.2e-7345.41RING/U-box superfamily protein [more]
AT3G13430.11.1e-5641.96RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 206..246
e-value: 7.0E-8
score: 42.2
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 205..247
e-value: 3.4E-13
score: 49.7
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 206..247
score: 12.386007
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 169..253
e-value: 2.0E-21
score: 77.5
IPR039525E3 ubiquitin-protein ligase RNF126-like, zinc-ribbonPFAMPF14369zinc_ribbon_9coord: 7..39
e-value: 3.4E-12
score: 46.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 253..325
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 96..121
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 268..325
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 42..62
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 106..121
NoneNo IPR availablePANTHERPTHR15710:SF22RING/U-BOX SUPERFAMILY PROTEINcoord: 148..303
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 148..303
coord: 3..147
NoneNo IPR availablePANTHERPTHR15710:SF22RING/U-BOX SUPERFAMILY PROTEINcoord: 3..147
NoneNo IPR availableCDDcd16669RING-H2_RNF181coord: 205..250
e-value: 1.91892E-22
score: 86.2705
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 196..251

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_4G017600.1CsaV3_4G017600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0061630 ubiquitin protein ligase activity