CsaV3_4G002660 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_4G002660
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	DNA-directed RNA polymerases II and V subunit 8A-like
Location	chr4: 1638784 .. 1642781 (+)
RNA-Seq Expression	CsaV3_4G002660
Synteny	CsaV3_4G002660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCAATGAATATAAACCCTAAACGTGAGTCATCGCCTTATACTCACCGAGTCACGCTGAACTTCCTCTTCAGCGAACGAGTCACGCCCCCCATCTTCTTCGACCAAGACCCTACGTTTGCCCTAACTCTTCGCCGCTCCTTGACGCCGTCGGTCAGTTTCAACCCTCTGTTTTTGTCCTCTCTCTCTCTACACACACACACACACACGTAGTTTATAATATCGAATTCGTTTCTGCTTTTATCGGGTTTTAGAGTGGCTTTGTAATCTGGTTGTTGATTTTTTTCTTATTCTTTCAATACAAACAATGTCGTTGATAGAATGTTAGCACTCCTAGTTCTTGGCTGCCTCTAATTCCATGGATAAGTATAGGTTAAAGTAAGTTTATGTGCAAGGCATTGTATATTTCCAAATGCACTTGTCAAGTTGATCTTCTTATTCAATTTTGCCTATATGGGCTGCTGGGTTTCTTATCTGTCTGTCGATGTTGGAGTTGGCTTTCCCTCCCTTGGTTAGTTTCCCATTTTTTGTCCTCATCACATGGCTGTTTTTTCTCATAGTTTCAGACGCTAAGGTGTTTGGAGTTTCGTTCCAAGCCATTTTATTCCGGGTTGTATTTGCTTTTATTTTCTTGTTTAGTTCAGTTATGGGCTATATTTGGTTGTATTTTTGTTATTTTGCTTTCATTTCATCGAAAAGCTCTTTTTTTTTTTAAAAAAAATAATAATTTGGGAGGGCCGTAAGCTAACCCTGTTAATTTTCTTGCATACGAAAACCTTGTATATCCTTTCTCGTACTTAATTTGAATCTGCAGAACCCATGTCAGATATTTTACTTAATATATTGATGTGTTCTTGCTTTGAAATATTTGTTCTTTAATTCCTGTATTCACTCCTCGATATGAATGTAAAATCGTTTACGCACGGACTTCATCCACTGCTTGACTATAACAAATGTCTTTTCACTCCACTTCTCTATTTGATGTGAATCTCCAACCTGGCTCTAACTCAGTTATGATGAGTGCTTGTCACAGAGATGTCAATGCTACGATTGAGGAGTCCTTCCTCAATCTGTCTTTTGGAAAGAAAGCCGTTTCTTTGGCCTGCCGACGTGTGTGCGGTGTTATGAGTCTTGTGGGATGAGAGGAACAGTAAGATGTTTAGGGTTGTGGAGAGGGACCCTAGGAAGCTTTGGGTCCCTTGTTCATTTTCATATTTCTCTTTGGGCTTCAACTTCAAAGACTTTTTGTAATTTTTCTGTAGGAGTGGTTTTGCATAGTTTAAACAGTCCTTGTAAAGGGTACCCTGCCTTTCTGTTGACTTGGTTTTTTTGTATGTCCGTGCATTCTTTCATTTTTTCTCACTGAAAGTGGTTATTTTCATTTAAAAACCCAGTGGGTTGCCTCCCCATTTACACCGAATGTTTAACACAGTTCTTATTTTTCAATCTTTTGCTTTGAAACCTGGCTTCCATTCACTAATGCGGTTCTTTTTTTGGCTGGTCTGGTCTGTTCTCTGATGGTCATACATTTTCTAGCACTGCTGGTTAATTTCTATTATTGCCCTGTTAATTTACAGGTTAGCGTTAACTTTTTGACAACAAAACTGCTGTTGAAGGATGGTTGAGCTTCTGTTTGACGATATTTTTAAGATTGAGCGATTAAACCCTGATGGTAAAAAGTTTGATAAAGGTACATTTTTGTATAGTAGTCACTTTCATTTCATTTATACAATATTTCCAGGAATGCTTATTAGCAGACTGTGGCAGTTTTATTGTTATATATCTGTAAAATCATTGTGCCATTCTTTAACTTTCTGATGGCGGTTGGAGATGTACTTCGTAGGCCTTTAAATCTCATCCATTCCTTATTTATTAACTTTTTAGAGGTTTCCTTTCAATCAGTTAGGATATCATCTGGAATATTATAACTCCATGTAAAATATGTGTTAAAAAAACTTTTCAACATATTTATGACATATTTTTTCACAATCTGTCCAACAGTTACTCGAATAGAGGCAAAGAGCGAGAAGTTTGACATGTTCATGCATCTAGATATAAATTCAGAGATATACCCCCTGAAAGAAGGTGAGAAGTTCTCGATGGCACTAGCTCCAACTCTGAATCTTGATGGAACCCCTGACACTGGATATTTTCTTCAGGTAACTTGTTCTTCTATTGATTTTAATCTGTTGCACACTGGATTTAACGTGGTGGTGTTGGTAAGAAAATGATGTCCTGGTAGGTTGAAGGATTTAGGCCTTGTTTGGCAACTTTTTAGGCCCTGTTTAGTAATCATTTGGTTTTTAGTTGAAAATTATGACTATAGACTTCAATTCCACATCTAAATTTATTGCTGTGTTATCTGCATTTTACAAGTATTTTTAAAAAGTGAAGCCAAAATTTGAAAACATGGAAAAGTAGTTTTTAAAAACTAAGAAATTGAGGGGAAAAGTTTAATTTTCAAAACTCAAAAACAAAAAGCGAAATAGAGTCTGAGTTTTTAGTTATTTGTTTTTGAAAATCTTATAAATATCCCTTTGTCCTCTAAAAAATTTGTTATCTACATTAAAACCAAGCGTAAATTTGAGAACTAAAAAATGTAGTTTTAAAAAACTTGTTTTACTTTTTAGAATTTGATTAAGAATTCAACTCTTAAGAAAAATATAAACCATTGCAAGAAATTGAGAGGAAGTAAGCTTAATCTTAAGAAACAAAAACTGAATAGTTACCAAATGGGGTCTTAGTAGTCCATTTTGTCATAAACTACTATATTTGAAATATCACTTTGAACATTAATAGGGAACAAGAAACTTTCAATCTGCTAGGAAATATATGCTTTCCATAGTTATTGCATATGTCAAGACTGGTCTTCATTGTTTTCCGACAACTATATTTGGTCTTGAGCATTTTCGACACTTGAATATGTCTACTTAACAATCATTAGGCTGCATAACAGGGCAATCGGAAGTCTCTTGCAGACAGATATGAATATGTCATGCAAGGGAAGCTGTTCAGAATTTCAGAAAGTTCTGGCCATGGCGGAAAAGCGTACGTGCTATAACTTCTCGAAACAATTTTAATGTGACACTTTAATTCCACTGTATGAGCATTAGAGAACCGAGAGCTTGGCATTGCCAAACTATACTTATCTAAATTTGAGCAAGAACCTGTCACGGTGCATTCTCCTGTCTATAATGGCTACAGAAGTGTATTGCGTGTGAATAAAATTCATGTTAGTTTCTTTTCCTGAACTGTAGGAACATATATATATTTATTCTAGTAGAATTGTTATCTAAAAAACTATATTTCATCTGAATCTAGTGAAATAGATGCTTCTTTTGGTGGACTTTTGATGATGCTGAAGGGAGACCCCTCACATTGCAGCAAGTTTGAACTTGACCAGAGGCTATTTCTTCTCATCAGGAAGGTGTGAAGCGCTTTCATTTGATTTGAACTCTAATGACTTGCTACAAAAGCTCTGGACATCTCTTATCGCTATTAGAACTGTGAATGTTTAGAAATATGTATTGTTAGAATCAAATTTCTATCTGTATCTGATTAAAGACTGGGCAGTGATCTTATCTTCTGAACTAAATTGATATCTTGCCTATAATGTCATGAATAGTGGCAGAACCTTTCGAGTTCTCGAAAGCGGAGTTTGAGCATGGTAGTATGTATAGTTTGTAGTGTTGGGCGGCCAACCTGAACAATTTTGGCAAGCTTGATTCCATTGCAGTTTGGGAGGGTGGAGACTGGCTAATGAAAGATTTAATAAATTACAAACATATTTAAGTGGGTGTGAGATTTGAATTTCTCACCTCAATGTTGCTATTAAACGTCTTTGAGATTTATTTCGTTGGTCTCTAGATTGTCATTATTTTTGTGGATGGAAAGTATATTTTCTTTATATGTACATGTATGTATTAAGTAATGATAAATAAATTTAGTTAGTTATTAATGTAACCATAATAAACACTTGAAACACCAATAATTTTTCTTT

mRNA sequence

ATGGTTGAGCTTCTGTTTGACGATATTTTTAAGATTGAGCGATTAAACCCTGATGGTAAAAAGTTTGATAAAGTTACTCGAATAGAGGCAAAGAGCGAGAAGTTTGACATGTTCATGCATCTAGATATAAATTCAGAGATATACCCCCTGAAAGAAGGTGAGAAGTTCTCGATGGCACTAGCTCCAACTCTGAATCTTGATGGAACCCCTGACACTGGATATTTTCTTCAGGGCAATCGGAAGTCTCTTGCAGACAGATATGAATATGTCATGCAAGGGAAGCTGTTCAGAATTTCAGAAAGTTCTGGCCATGGCGGAAAAGCTGAAATAGATGCTTCTTTTGGTGGACTTTTGATGATGCTGAAGGGAGACCCCTCACATTGCAGCAAGTTTGAACTTGACCAGAGGCTATTTCTTCTCATCAGGAAGGTGTGA

Coding sequence (CDS)

ATGGTTGAGCTTCTGTTTGACGATATTTTTAAGATTGAGCGATTAAACCCTGATGGTAAAAAGTTTGATAAAGTTACTCGAATAGAGGCAAAGAGCGAGAAGTTTGACATGTTCATGCATCTAGATATAAATTCAGAGATATACCCCCTGAAAGAAGGTGAGAAGTTCTCGATGGCACTAGCTCCAACTCTGAATCTTGATGGAACCCCTGACACTGGATATTTTCTTCAGGGCAATCGGAAGTCTCTTGCAGACAGATATGAATATGTCATGCAAGGGAAGCTGTTCAGAATTTCAGAAAGTTCTGGCCATGGCGGAAAAGCTGAAATAGATGCTTCTTTTGGTGGACTTTTGATGATGCTGAAGGGAGACCCCTCACATTGCAGCAAGTTTGAACTTGACCAGAGGCTATTTCTTCTCATCAGGAAGGTGTGA

Protein sequence

MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMALAPTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMMLKGDPSHCSKFELDQRLFLLIRKV*

Homology

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BLAST of CsaV3_4G002660 vs. NCBI nr
Match: XP_004152137.1 (DNA-directed RNA polymerases II and V subunit 8A [Cucumis sativus] >KGN53006.1 hypothetical protein Csa_014470 [Cucumis sativus])

HSP 1 Score: 294.3 bits (752), Expect = 5.8e-76
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

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BLAST of CsaV3_4G002660 vs. NCBI nr
Match: XP_008454076.1 (PREDICTED: DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo])

HSP 1 Score: 293.9 bits (751), Expect = 7.6e-76
Identity = 143/144 (99.31%), Postives = 144/144 (100.00%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLD+NSEIYPLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

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BLAST of CsaV3_4G002660 vs. NCBI nr
Match: XP_038879439.1 (DNA-directed RNA polymerases II and V subunit 8A-like [Benincasa hispida] >XP_038879448.1 DNA-directed RNA polymerases II and V subunit 8A-like [Benincasa hispida])

HSP 1 Score: 290.8 bits (743), Expect = 6.5e-75
Identity = 143/144 (99.31%), Postives = 143/144 (99.31%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

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BLAST of CsaV3_4G002660 vs. NCBI nr
Match: XP_022932746.1 (DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita moschata] >XP_022932747.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita moschata] >XP_023538873.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita pepo subsp. pepo] >XP_023538874.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 287.7 bits (735), Expect = 5.5e-74
Identity = 141/144 (97.92%), Postives = 142/144 (98.61%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDIN+EIY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVM GKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMHGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

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BLAST of CsaV3_4G002660 vs. NCBI nr
Match: KAG6597681.1 (DNA-directed RNA polymerases II and V subunit 8A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 287.7 bits (735), Expect = 5.5e-74
Identity = 141/144 (97.92%), Postives = 142/144 (98.61%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDIN+EIY LKEGEKFSMAL
Sbjct: 178 MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 237

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVM GKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 238 APTLNLDGTPDTGYFLQGNRKSLADRYEYVMHGKLFRISESSGHGGKAEIDASFGGLLMM 297

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 298 LKGDPSHCSKFELDQRLFLLIRKV 321

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BLAST of CsaV3_4G002660 vs. ExPASy Swiss-Prot
Match: O81097 (DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702 GN=NRPB8A PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 5.0e-54
Identity = 95/141 (67.38%), Postives = 121/141 (85.82%), Query Frame = 0


Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMALAPT 63
           +LF+DIF +++L+PDGKKFDKVTR++A S   +MFMHLD+N+E+YPL  G+KF++ALAPT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMMLKG 123
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE+  SFGGLLM+LKG
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLKG 125

Query: 124 DPSHCSKFELDQRLFLLIRKV 145
           DP+H S FELDQRLFLL+RK+
Sbjct: 126 DPAHISHFELDQRLFLLMRKL 146

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BLAST of CsaV3_4G002660 vs. ExPASy Swiss-Prot
Match: Q9M1A8 (DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=3702 GN=NRPB8B PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 6.5e-54
Identity = 93/141 (65.96%), Postives = 122/141 (86.52%), Query Frame = 0


Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMALAPT 63
           ++F+DIF +++L+PDGKKFDKVTR+EA+S   +MFMHLD+N+E+YPL  G+KF++A+APT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMMLKG 123
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE+  SFGGLLM+L+G
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLQG 125

Query: 124 DPSHCSKFELDQRLFLLIRKV 145
           DP+H S FELDQRLFLL+RK+
Sbjct: 126 DPAHISHFELDQRLFLLMRKL 146

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BLAST of CsaV3_4G002660 vs. ExPASy Swiss-Prot
Match: Q54YW8 (DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium discoideum OX=44689 GN=polr2h PE=3 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 4.1e-32
Identity = 66/143 (46.15%), Postives = 98/143 (68.53%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           M  +LF+D F+++ ++PDGKKFD+V+R    SE ++M + +DI + IYPL   E+F M L
Sbjct: 1   MTSILFEDTFEVKEIDPDGKKFDRVSRFVCYSENYEMDLQIDIATHIYPLGH-ERFKMVL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           A +LNLDG+ D       N+ SLAD+Y+YVM GK+F+  + +    K  + ASFGGLL++
Sbjct: 61  ASSLNLDGSSDNSGSYDTNKPSLADKYDYVMYGKIFKYQKENS-SSKVSVFASFGGLLVL 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRK 144
           L+GDP +    ELD R++LLI+K
Sbjct: 121 LQGDPRYLPGIELDARIYLLIKK 141

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BLAST of CsaV3_4G002660 vs. ExPASy Swiss-Prot
Match: A8XT81 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis briggsae OX=6238 GN=rpb-8 PE=3 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.1e-29
Identity = 65/145 (44.83%), Postives = 94/145 (64.83%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           M  ++FDD+F ++ ++PDGKKFD+V+R    +E F M + +DINS+IYPLK+ +KF + L
Sbjct: 1   MAGIIFDDMFSVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKFRLVL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRI--SESSGHGGKAEIDASFGGLL 120
           A TL  DG  D G +          +YEYVM GK++R+   +S   GG+    ASFGGLL
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDSGTDGGQLAAYASFGGLL 120

Query: 121 MMLKGDPSHCSKFELDQRLFLLIRK 144
           M LKG+  +   FE+D  L+LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

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BLAST of CsaV3_4G002660 vs. ExPASy Swiss-Prot
Match: Q19826 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis elegans OX=6239 GN=rpb-8 PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.5e-29
Identity = 65/145 (44.83%), Postives = 94/145 (64.83%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           M  ++FDD+FK++ ++PDGKKFD+V+R    +E F M + +DINS+IYPLK+ +K  + L
Sbjct: 1   MAGIIFDDMFKVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKVRLVL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRI--SESSGHGGKAEIDASFGGLL 120
           A TL  DG  D G +          +YEYVM GK++R+   ++   GGK    ASFGGLL
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDTGTDGGKLAAYASFGGLL 120

Query: 121 MMLKGDPSHCSKFELDQRLFLLIRK 144
           M LKG+  +   FE+D  L+LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

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BLAST of CsaV3_4G002660 vs. ExPASy TrEMBL
Match: A0A0A0KYV5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G011050 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.8e-76
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

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BLAST of CsaV3_4G002660 vs. ExPASy TrEMBL
Match: A0A1S3BXS2 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo OX=3656 GN=LOC103494598 PE=3 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 3.7e-76
Identity = 143/144 (99.31%), Postives = 144/144 (100.00%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLD+NSEIYPLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

[Close]

BLAST of CsaV3_4G002660 vs. ExPASy TrEMBL
Match: A0A6J1EXM3 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucurbita moschata OX=3662 GN=LOC111439203 PE=3 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 2.6e-74
Identity = 141/144 (97.92%), Postives = 142/144 (98.61%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDIN+EIY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVM GKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMHGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

[Close]

BLAST of CsaV3_4G002660 vs. ExPASy TrEMBL
Match: A0A6J1CWD5 (DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015176 PE=3 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 4.5e-74
Identity = 139/144 (96.53%), Postives = 143/144 (99.31%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDIN+EIY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGY+LQGNRKSLADRYEY+MQGKLFRISESSGHGG+AEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYYLQGNRKSLADRYEYIMQGKLFRISESSGHGGRAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

[Close]

BLAST of CsaV3_4G002660 vs. ExPASy TrEMBL
Match: A0A6J1I8Y6 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucurbita maxima OX=3661 GN=LOC111470753 PE=3 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 2.9e-73
Identity = 140/144 (97.22%), Postives = 141/144 (97.92%), Query Frame = 0


Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDIN+EIY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVM GKLFRISESSGH GKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMHGKLFRISESSGHVGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

[Close]

BLAST of CsaV3_4G002660 vs. TAIR 10
Match: AT1G54250.1 (RNA polymerase Rpb8 )

HSP 1 Score: 211.8 bits (538), Expect = 3.6e-55
Identity = 95/141 (67.38%), Postives = 121/141 (85.82%), Query Frame = 0


Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMALAPT 63
           +LF+DIF +++L+PDGKKFDKVTR++A S   +MFMHLD+N+E+YPL  G+KF++ALAPT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMMLKG 123
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE+  SFGGLLM+LKG
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLKG 125

Query: 124 DPSHCSKFELDQRLFLLIRKV 145
           DP+H S FELDQRLFLL+RK+
Sbjct: 126 DPAHISHFELDQRLFLLMRKL 146

[Close]

BLAST of CsaV3_4G002660 vs. TAIR 10
Match: AT3G59600.1 (RNA polymerase Rpb8 )

HSP 1 Score: 211.5 bits (537), Expect = 4.6e-55
Identity = 93/141 (65.96%), Postives = 122/141 (86.52%), Query Frame = 0


Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMALAPT 63
           ++F+DIF +++L+PDGKKFDKVTR+EA+S   +MFMHLD+N+E+YPL  G+KF++A+APT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMMLKG 123
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE+  SFGGLLM+L+G
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLQG 125

Query: 124 DPSHCSKFELDQRLFLLIRKV 145
           DP+H S FELDQRLFLL+RK+
Sbjct: 126 DPAHISHFELDQRLFLLMRKL 146

The following BLAST results are available for this feature:

BLAST of CsaV3_4G002660 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152137.1	5.8e-76	100.00	DNA-directed RNA polymerases II and V subunit 8A [Cucumis sativus] >KGN53006.1 h...	[more]
XP_008454076.1	7.6e-76	99.31	PREDICTED: DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo]	[more]
XP_038879439.1	6.5e-75	99.31	DNA-directed RNA polymerases II and V subunit 8A-like [Benincasa hispida] >XP_03...	[more]
XP_022932746.1	5.5e-74	97.92	DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita moschata] >XP_0...	[more]
KAG6597681.1	5.5e-74	97.92	DNA-directed RNA polymerases II and V subunit 8A, partial [Cucurbita argyrosperm...	[more]

BLAST of CsaV3_4G002660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81097	5.0e-54	67.38	DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702...	[more]
Q9M1A8	6.5e-54	65.96	DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=...	[more]
Q54YW8	4.1e-32	46.15	DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium disc...	[more]
A8XT81	1.1e-29	44.83	Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab...	[more]
Q19826	1.5e-29	44.83	Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab...	[more]

BLAST of CsaV3_4G002660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KYV5	2.8e-76	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G011050 PE=3 SV=1	[more]
A0A1S3BXS2	3.7e-76	99.31	DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo OX=3656 GN...	[more]
A0A6J1EXM3	2.6e-74	97.92	DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucurbita moschata OX=3...	[more]
A0A6J1CWD5	4.5e-74	96.53	DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 OS=Momordica ch...	[more]
A0A6J1I8Y6	2.9e-73	97.22	DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucurbita maxima OX=366...	[more]

BLAST of CsaV3_4G002660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G54250.1	3.6e-55	67.38	RNA polymerase Rpb8	[more]
AT3G59600.1	4.6e-55	65.96	RNA polymerase Rpb8	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005570	RNA polymerase, Rpb8	SMART	SM00658	rpol8neu	coord: 2..143 e-value: 3.7E-68 score: 242.4
IPR005570	RNA polymerase, Rpb8	PIRSF	PIRSF000779	RPB8	coord: 1..144 e-value: 6.9E-50 score: 166.8
IPR005570	RNA polymerase, Rpb8	PFAM	PF03870	RNA_pol_Rpb8	coord: 7..143 e-value: 1.0E-49 score: 168.3
IPR005570	RNA polymerase, Rpb8	PANTHER	PTHR10917	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3	coord: 1..144
None	No IPR available	GENE3D	2.40.50.140		coord: 6..144 e-value: 3.5E-56 score: 191.2
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 4..143

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_4G002660.1	CsaV3_4G002660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated