CsaV3_4G000740 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_4G000740 vs. NCBI nr
Match: KAE8649042.1 (hypothetical protein Csa_015273 [Cucumis sativus])

HSP 1 Score: 947.2 bits (2447), Expect = 5.4e-272
Identity = 471/471 (100.00%), Postives = 471/471 (100.00%), Query Frame = 0

Query: 1   MANSQILLSPFSCLPRLPSYPIQSPSKNRFQIHSTFAKFDNFLDLKPESKPQPLKLDLPL 60
           MANSQILLSPFSCLPRLPSYPIQSPSKNRFQIHSTFAKFDNFLDLKPESKPQPLKLDLPL
Sbjct: 1   MANSQILLSPFSCLPRLPSYPIQSPSKNRFQIHSTFAKFDNFLDLKPESKPQPLKLDLPL 60

Query: 61  FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL 120
           FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL
Sbjct: 61  FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL 120

Query: 121 GLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEECISKGVKFHKAKVWEI 180
           GLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEECISKGVKFHKAKVWEI
Sbjct: 121 GLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEECISKGVKFHKAKVWEI 180

Query: 181 NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTKSRPPPIRSKQNGSNGLERHSFEQRTI 240
           NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTKSRPPPIRSKQNGSNGLERHSFEQRTI
Sbjct: 181 NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTKSRPPPIRSKQNGSNGLERHSFEQRTI 240

Query: 241 PKTRQQKIPNIPLRNALRFKPNLSRRDFPRRPAGDAVHSSEKEDGGQVAAFGDKGEENYR 300
           PKTRQQKIPNIPLRNALRFKPNLSRRDFPRRPAGDAVHSSEKEDGGQVAAFGDKGEENYR
Sbjct: 241 PKTRQQKIPNIPLRNALRFKPNLSRRDFPRRPAGDAVHSSEKEDGGQVAAFGDKGEENYR 300

Query: 301 RGEMCDSDGGCSAEDAAGSGGGRWGGGDGEPASGYAVVKGMGAAGRIARVAAEGVGGGGG 360
           RGEMCDSDGGCSAEDAAGSGGGRWGGGDGEPASGYAVVKGMGAAGRIARVAAEGVGGGGG
Sbjct: 301 RGEMCDSDGGCSAEDAAGSGGGRWGGGDGEPASGYAVVKGMGAAGRIARVAAEGVGGGGG 360

Query: 361 RMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILVRLDLDGIRGFFNAFFDLEPSYWEG 420
           RMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILVRLDLDGIRGFFNAFFDLEPSYWEG
Sbjct: 361 RMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILVRLDLDGIRGFFNAFFDLEPSYWEG 420

Query: 421 LLSSRLSLLELGMLSLSLYQHASFRSKLDILTKSPLPLLQMMANLTLHALG 472
           LLSSRLSLLELGMLSLSLYQHASFRSKLDILTKSPLPLLQMMANLTLHALG
Sbjct: 421 LLSSRLSLLELGMLSLSLYQHASFRSKLDILTKSPLPLLQMMANLTLHALG 471

BLAST of CsaV3_4G000740 vs. NCBI nr
Match: XP_011652931.2 (LOW QUALITY PROTEIN: capsanthin/capsorubin synthase, chromoplastic [Cucumis sativus])

HSP 1 Score: 688.3 bits (1775), Expect = 4.5e-194
Identity = 378/495 (76.36%), Postives = 394/495 (79.60%), Query Frame = 0

Query: 1   MANSQILLSPFSCLPRLPSYPIQSPSKNRFQIHSTFAKFDNFLDLKPESKPQPLKLDLPL 60
           MANSQILLSPFSCLPRLPSYPIQSPSKNRFQIHSTFAKFDNFLDLKPESKPQPLKLDLPL
Sbjct: 1   MANSQILLSPFSCLPRLPSYPIQSPSKNRFQIHSTFAKFDNFLDLKPESKPQPLKLDLPL 60

Query: 61  FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL 120
           FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL
Sbjct: 61  FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL 120

Query: 121 GLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEECISKGVKFHKAKVWEI 180
           GLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEECISKGVKFHKAKVWEI
Sbjct: 121 GLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEECISKGVKFHKAKVWEI 180

Query: 181 NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTK-SRPPPIRSKQNGSNGL----ERHSF 240
           NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTK S   P       ++G+    + H F
Sbjct: 181 NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTKYSESNPRNCAFQIAHGILAEVDHHPF 240

Query: 241 EQRTI-------------PKTRQ--QKIPN----IPLRNALRFKPNLSRRDFPRRPAGDA 300
           +   +             P  RQ  +K P     +P  + L F    S    P  P    
Sbjct: 241 DLNKMVLMDWRDTHLNNEPYLRQDNKKFPTFLYAMPFDSNLIFLEETSLAGRPAMP---- 300

Query: 301 VHSSEKEDGGQVAAFGDKGEENYRRGEMCDSDGGCSAEDAAGSGGGRWGGGDGEPASGYA 360
             + +K    ++   G K +      +     GG   +      G     G   PASGYA
Sbjct: 301 YTAVKKRMAARLRHLGIKVKRIIEEEKCVIPMGGALPKMPQAVVGVGGVAGMVXPASGYA 360

Query: 361 VVKGMGAAGRIARVAAEGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILV 420
           VVKGMGAAGRIARVAAEGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILV
Sbjct: 361 VVKGMGAAGRIARVAAEGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILV 420

Query: 421 RLDLDGIRGFFNAFFDLEPSYWEGLLSSRLSLLELGMLSLSLYQHASFRSKLDILTKSPL 472
           RLDLDGIRGFFNAFFDLEPSYWEGLLSSRLSLLELGMLSLSLYQHASFRSKLDILTKSPL
Sbjct: 421 RLDLDGIRGFFNAFFDLEPSYWEGLLSSRLSLLELGMLSLSLYQHASFRSKLDILTKSPL 480

BLAST of CsaV3_4G000740 vs. NCBI nr
Match: XP_008454352.1 (PREDICTED: capsanthin/capsorubin synthase, chromoplastic-like [Cucumis melo])

HSP 1 Score: 649.8 bits (1675), Expect = 1.8e-182
Identity = 360/492 (73.17%), Postives = 377/492 (76.63%), Query Frame = 0

Query: 1   MANSQILLSPFSCLPRLPSYPIQSPSKNRFQIHSTFAKFDNFLDLKPESKPQPLKLDLPL 60
           MANS+ LLSPF  LP+LPS PIQSPSKN  QIHSTFAKFDNFLDLKPESKP+PLKLDLPL
Sbjct: 1   MANSRFLLSPFFSLPQLPSNPIQSPSKNHLQIHSTFAKFDNFLDLKPESKPKPLKLDLPL 60

Query: 61  FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL 120
           FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL
Sbjct: 61  FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL 120

Query: 121 GLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEECISKGVKFHKAKVWEI 180
           GLEDCLHKTWPMAS+YLSDEKVKYLDRPYAQVSRKKLKMKLMEECI KGVKFHKAKVWEI
Sbjct: 121 GLEDCLHKTWPMASLYLSDEKVKYLDRPYAQVSRKKLKMKLMEECIRKGVKFHKAKVWEI 180

Query: 181 NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTK-SRPPPIRSKQNGSNGL----ERHSF 240
           NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTK S   P       ++G+    + H F
Sbjct: 181 NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTKYSDSNPRNCAFQIAHGILAEVDHHPF 240

Query: 241 EQRTI-------------PKTRQQKIPNIPLRNALRFKPN---LSRRDFPRRPAGDAVHS 300
           +   +             P  RQ          A+ F  N   L       RPA      
Sbjct: 241 DLNKMVLMDWRDTHLNNEPYLRQHNKEFPTFLYAMPFDSNLIFLEETSLVGRPALPYT-V 300

Query: 301 SEKEDGGQVAAFGDKGEENYRRGEMCDSDGGCSAEDAAGSGGGRWGGGDGEPASGYAVVK 360
            +K    ++   G K +      +     GG   E      G     G   PASGYAVVK
Sbjct: 301 VKKRMAARLRHLGIKVKRILEEEKCVIPMGGALPEMPQAVVGVGGVAGMVNPASGYAVVK 360

Query: 361 GMGAAGRIARVAAEGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILVRLD 420
           GM AAGRIARVA EGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEIL+RLD
Sbjct: 361 GMAAAGRIARVAGEGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILMRLD 420

Query: 421 LDGIRGFFNAFFDLEPSYWEGLLSSRLSLLELGMLSLSLYQHASFRSKLDILTKSPLPLL 472
           LDGIRGFFNAFFDLEP YWEG LSSRLSLLEL MLSLSLYQHASFRSKLDILTKSPLPLL
Sbjct: 421 LDGIRGFFNAFFDLEPFYWEGFLSSRLSLLELAMLSLSLYQHASFRSKLDILTKSPLPLL 480

BLAST of CsaV3_4G000740 vs. NCBI nr
Match: XP_038903700.1 (capsanthin/capsorubin synthase, chromoplastic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 573.9 bits (1478), Expect = 1.2e-159
Identity = 322/493 (65.31%), Postives = 363/493 (73.63%), Query Frame = 0

Query: 1   MANSQILLSPFSCLPRLPSYPIQSPSKNRFQIHSTFAKFDNFLDLKPESKPQPLKLDLPL 60
           MANSQ LLSPFS LP+LPS PI SPS N FQIH +F KF NFLDLKPESKP+PLKLDLPL
Sbjct: 34  MANSQALLSPFS-LPQLPSSPIHSPSTNHFQIHCSFGKFGNFLDLKPESKPEPLKLDLPL 93

Query: 61  FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL 120
           FHPY DRSRFDVVV+GAGP GLR+AEQLGGFGIKVCC+DPSPLSPW NNYGVWVDEFE L
Sbjct: 94  FHPYSDRSRFDVVVIGAGPAGLRLAEQLGGFGIKVCCMDPSPLSPWPNNYGVWVDEFEAL 153

Query: 121 GLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEECISKGVKFHKAKVWEI 180
           G +DC HKTWPMA+VYLSD KVKYLDRPYAQVSRKKLKMKLMEEC+ KGVKFHKAKVWEI
Sbjct: 154 GFQDCFHKTWPMATVYLSDRKVKYLDRPYAQVSRKKLKMKLMEECVRKGVKFHKAKVWEI 213

Query: 181 NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTK-SRPPPIRSKQNGSNGL----ERHSF 240
            HQ+FESSVSCNDGTEIK+NLVIDASGF SKFT+ S+  P       ++G+    ERH F
Sbjct: 214 KHQEFESSVSCNDGTEIKANLVIDASGFASKFTEYSKSKPRNCGFQIAHGILAEVERHPF 273

Query: 241 EQRTI-------------PKTRQQKIPNIPLRNALRFKPNLSRRDFPRRPAGDAVHSSE- 300
           +   +             P  RQ    +     A+ F  NL   +        A+  +E 
Sbjct: 274 DLNKMVLMDWRDTHLGNEPYLRQDNKKSPTFLYAMPFDSNLIFMEETSLVGRPALPYTEV 333

Query: 301 -KEDGGQVAAFGDKGEENYRRGEMCDSDGGCSAE--DAAGSGGGRWGGGDGEPASGYAVV 360
            +    ++   G K +      +     GG   E   AA + GG    G   PA+GYAVV
Sbjct: 334 KRRMAARLRHLGIKVKRILEEEKCVIPMGGPLPEMPQAAVAAGG--VAGMVNPATGYAVV 393

Query: 361 KGMGAAGRIARVAAEGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILVRL 420
            GM AA R+AR+AAEG+ GGGGRMIRGRAL+GRVWEGVWPME+R+VREFYCFGME LVRL
Sbjct: 394 GGMAAAPRVARMAAEGL-GGGGRMIRGRALQGRVWEGVWPMEKRSVREFYCFGMETLVRL 453

Query: 421 DLDGIRGFFNAFFDLEPSYWEGLLSSRLSLLELGMLSLSLYQHASFRSKLDILTKSPLPL 472
           DLDG+R FF+AFFDLEP YWEG LSSRLSL EL MLSLSLY HAS  SK+DILTK PLPL
Sbjct: 454 DLDGMRRFFDAFFDLEPFYWEGFLSSRLSLWELVMLSLSLYGHASLMSKVDILTKCPLPL 513

BLAST of CsaV3_4G000740 vs. NCBI nr
Match: TYK29501.1 (capsanthin/capsorubin synthase, chromoplastic-like [Cucumis melo var. makuwa])

HSP 1 Score: 565.5 bits (1456), Expect = 4.4e-157
Identity = 325/480 (67.71%), Postives = 346/480 (72.08%), Query Frame = 0

Query: 1   MANSQILLSPFSCLPRLPSYPIQSPSKNRFQIHSTFAKFDNFLDLKPESKPQPLKLDLPL 60
           MANS+ LLSPF  LP+LPS PIQSPSKN  QIHSTFAKFDNFLDLKPESKP+PLKLDLPL
Sbjct: 1   MANSRFLLSPFFSLPQLPSNPIQSPSKNHLQIHSTFAKFDNFLDLKPESKPKPLKLDLPL 60

Query: 61  FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL 120
           FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL
Sbjct: 61  FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL 120

Query: 121 GLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEECISKGVKFHKAKVWEI 180
           GLEDCLHKTWPMAS+YLSDEKVKYLDRPYAQVSRKKLKMKLMEECI KGVKFHKAKVWEI
Sbjct: 121 GLEDCLHKTWPMASLYLSDEKVKYLDRPYAQVSRKKLKMKLMEECIRKGVKFHKAKVWEI 180

Query: 181 NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTK-SRPPPIRSKQNGSNGL----ERHSF 240
           NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTK S   P       ++G+    + H F
Sbjct: 181 NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTKYSDSNPRNCAFQIAHGILAEVDHHPF 240

Query: 241 E--QRTIPKTRQQKIPNIPL--RNALRFKPNLSRRDFPRRPAGDAVHSSEKEDGGQVAAF 300
           +  +  +   R   + N P   ++   F   L    F      + +   E    G+ A  
Sbjct: 241 DLNKMVLMDWRDTHLNNEPYLRQHNKEFPTFLYAMPFD----SNLIFLEETSLVGRPAL- 300

Query: 301 GDKGEENYRRGEMCDSDGGCSAEDAAGSGGGRWGGGDGEPASGYAVVKGMGAAGRIARVA 360
                                 +    +     G            V  MG A      A
Sbjct: 301 -----------------PYTVVKKRMAARLRHLGIKVKRILEEEKCVIPMGGALPEMPQA 360

Query: 361 AEGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILVRLDLDGIRGFFNAFF 420
             GVGG  G +    A   RVWEGVWPMEERNVREFYCFGMEIL+RLDLDGIRGFFNAFF
Sbjct: 361 VVGVGGVAGMV--NPASGXRVWEGVWPMEERNVREFYCFGMEILMRLDLDGIRGFFNAFF 420

Query: 421 DLEPSYWEGLLSSRLSLLELGMLSLSLYQHASFRSKLDILTKSPLPLLQMMANLTLHALG 472
           DLEP YWEG LSSRLSLLEL MLSLSLYQHASFRSKLDILTKSPLPLLQMMANLTL ALG
Sbjct: 421 DLEPFYWEGFLSSRLSLLELAMLSLSLYQHASFRSKLDILTKSPLPLLQMMANLTLQALG 456

BLAST of CsaV3_4G000740 vs. ExPASy Swiss-Prot
Match: Q9SEA0 (Capsanthin/capsorubin synthase, chromoplastic OS=Citrus sinensis OX=2711 GN=CCS PE=3 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 6.7e-100
Identity = 222/461 (48.16%), Postives = 277/461 (60.09%), Query Frame = 0

Query: 37  AKFDNFLDLKPESKPQPLKLDLPLFHPYYDRSRFDVVVVGAGPIGLRVAEQLGG-FGIKV 96
           +KF NFL+L PES P+ L  DLP FHP  DR R+DV+++G GP GLR+AEQ+     +KV
Sbjct: 54  SKFGNFLELTPESVPEFLDFDLPWFHP-SDRIRYDVIIIGTGPAGLRLAEQVSSRHSVKV 113

Query: 97  CCVDPSPLSPWSNNYGVWVDEFEDLGLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRK 156
           CCVDPSPLS W NNYGVWVDEFED+GL DCL KTWPM  V+++D K KYLDRPY +VSR 
Sbjct: 114 CCVDPSPLSTWPNNYGVWVDEFEDIGLVDCLDKTWPMTCVFINDHKTKYLDRPYGRVSRN 173

Query: 157 KLKMKLMEECISKGVKFHKAKVWEINHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTKS 216
            LK KL+E C+S GVKFHKAKVW +NHQ+FESS+ C+DG EIK++L++DASGF S F + 
Sbjct: 174 ILKTKLLENCVSNGVKFHKAKVWHVNHQEFESSIVCDDGNEIKASLIVDASGFASSFVEY 233

Query: 217 RPPPIRSKQNGSNGL---ERHSF--EQRTIPKTRQQKIPNIPLRN-----------ALRF 276
             P     Q     L   E H F  ++  +   R   + N P              A+ F
Sbjct: 234 DKPRNHGYQIAHGILAEVESHPFDLDKMVLMDWRDSHLGNEPYLRASNLKLPTFLYAMPF 293

Query: 277 KPN---LSRRDFPRRPAGDAVHSSEKEDGGQVAA----FGDKGEENYRRGEMCDSDGG-- 336
             N   L       RP       S KE   ++AA     G + +      +     GG  
Sbjct: 294 DSNLVFLEETSLVSRPV-----LSYKEVKSRMAARLRHMGIRVKRVIEDEKCLIPMGGPL 353

Query: 337 -CSAEDAAGSGGGRWGGGDGEPASGYAVVKGMGAAGRIARVAAEGVGGGGGRMIRGRALE 396
               +     GG     G   PA+GY V + M  A  +A   AE +  G  RMIRGR L 
Sbjct: 354 PVIPQSVMAIGG---TSGLIHPATGYMVARTMALAPALADAIAECL--GSTRMIRGRPLH 413

Query: 397 GRVWEGVWPMEERNVREFYCFGMEILVRLDLDGIRGFFNAFFDLEPSYWEGLLSSRLSLL 456
            +VW G+WP++ R  REFY FGME L++LDL G R FF+AFFDL P YW G LSSRLSL 
Sbjct: 414 QKVWNGLWPIDRRCNREFYSFGMETLLKLDLKGTRRFFDAFFDLNPYYWHGFLSSRLSLA 473

Query: 457 ELGMLSLSLYQHASFRSKLDILTKSPLPLLQMMANLTLHAL 471
           EL  LSLSL+ HAS  S+LDI+TK P+PL++MM NL L  +
Sbjct: 474 ELAGLSLSLFGHASNSSRLDIVTKCPVPLVKMMGNLALETI 503

BLAST of CsaV3_4G000740 vs. ExPASy Swiss-Prot
Match: K4C9E2 (Neoxanthin synthase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NSY PE=1 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 1.6e-93
Identity = 201/465 (43.23%), Postives = 272/465 (58.49%), Query Frame = 0

Query: 39  FDNFLDLKPESKPQPLKLDLPLFHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCV 98
           F +FLDL P SKP+ L +++    P  +R++FDV+++GAGP GLR+AEQ+  +GIKVCCV
Sbjct: 51  FCSFLDLAPTSKPESLDVNISWVDPNSNRAQFDVIIIGAGPAGLRLAEQVSKYGIKVCCV 110

Query: 99  DPSPLSPWSNNYGVWVDEFEDLGLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLK 158
           DPSPLS W NNYGVWVDEFE+LGLEDCL   WPM  V+++D K KYL RPY +VSRKKLK
Sbjct: 111 DPSPLSMWPNNYGVWVDEFENLGLEDCLDHKWPMTCVHINDNKTKYLGRPYGRVSRKKLK 170

Query: 159 MKLMEECISKGVKFHKAKVWEINHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTKSRPP 218
           +KL+  C+   VKF+KAKVW++ H++FESS+ C+DG +I+ +LV+DASGF S F +   P
Sbjct: 171 LKLLNSCVENRVKFYKAKVWKVEHEEFESSIVCDDGKKIRGSLVVDASGFASDFIEYDRP 230

Query: 219 PIRSKQNGSNGL---ERHSF--EQRTIPKTRQQKIPNIPL-------------------- 278
                Q     L   + H F  ++  +   R   + N P                     
Sbjct: 231 RNHGYQIAHGVLVEVDNHPFDLDKMVLMDWRDSHLGNEPYLRVNNAKEPTFLYAMPFDRD 290

Query: 279 -----RNALRFKPNLSRRDFPRRPAGDAVHSSEKEDGGQVAAFGDKGEENYRRGEMCDSD 338
                  +L  +P LS  +  RR      H             G K +      +     
Sbjct: 291 LVFLEETSLVSRPVLSYMEVKRRMVARLRH------------LGIKVKSVIEEEKCVIPM 350

Query: 339 GG---CSAEDAAGSGGGRWGGGDGEPASGYAVVKGMGAAGRIARVAAEGVGGGGGRMIRG 398
           GG      ++    GG     G   P++GY V + M  A  +A    EG+  G  RMIRG
Sbjct: 351 GGPLPRIPQNVMAIGG---NSGIVHPSTGYMVARSMALAPVLAEAIVEGL--GSTRMIRG 410

Query: 399 RALEGRVWEGVWPMEERNVREFYCFGMEILVRLDLDGIRGFFNAFFDLEPSYWEGLLSSR 458
             L  RVW G+WP++ R VRE Y FGME L++LDL G R  F+AFFDL+P YW+G LSSR
Sbjct: 411 SQLYHRVWNGLWPLDRRCVRECYSFGMETLLKLDLKGTRRLFDAFFDLDPKYWQGFLSSR 470

Query: 459 LSLLELGMLSLSLYQHASFRSKLDILTKSPLPLLQMMANLTLHAL 471
           LS+ ELG+LSL L+ H S  ++LDI+TK PLPL++++ NL + +L
Sbjct: 471 LSVKELGLLSLCLFGHGSNMTRLDIVTKCPLPLVRLIGNLAIESL 498

BLAST of CsaV3_4G000740 vs. ExPASy Swiss-Prot
Match: Q42435 (Capsanthin/capsorubin synthase, chromoplastic OS=Capsicum annuum OX=4072 GN=CCS PE=1 SV=2)

HSP 1 Score: 333.2 bits (853), Expect = 4.8e-90
Identity = 207/507 (40.83%), Postives = 287/507 (56.61%), Query Frame = 0

Query: 5   QILLSPFSCLPRLPSYPIQSPSKNRFQIHSTF-------------------AKFDNFLDL 64
           + LL PF      P   I +P+   F+ +STF                     F +FLDL
Sbjct: 2   ETLLKPFPS----PLLSIPTPNMYSFKHNSTFPNPTKQKDSRKFHYRNKSSTHFCSFLDL 61

Query: 65  KPESKPQPLKLDLPLFHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSP 124
            P SKP+ L +++       DR+ FDV+++G GP GLR+AEQ+  +GIKVCCVDPSPLS 
Sbjct: 62  APTSKPESLDVNISWVDTDLDRAEFDVIIIGTGPAGLRLAEQVSKYGIKVCCVDPSPLSM 121

Query: 125 WSNNYGVWVDEFEDLGLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEEC 184
           W NNYGVWVDEFE LGLEDCL   WP++ V++SD K KYLDRPY +VSRKKLK+KL+  C
Sbjct: 122 WPNNYGVWVDEFEKLGLEDCLDHKWPVSCVHISDHKTKYLDRPYGRVSRKKLKLKLLNSC 181

Query: 185 ISKGVKFHKAKVWEINHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTKSRPPPIRSKQN 244
           +   VKF+KAKV ++ H++FESS+ C+DG +I  +L++DASG+ S F +   P     Q 
Sbjct: 182 VENRVKFYKAKVLKVKHEEFESSIVCDDGRKISGSLIVDASGYASDFIEYDKPRNHGYQV 241

Query: 245 GSNGL---ERHSF--EQRTIPKTRQQKIPNIP---LRN--------ALRFKPN---LSRR 304
               L   + H F  ++  +   R   + N P   ++N        A+ F  N   L   
Sbjct: 242 AHGILAEVDNHPFDLDKMMLMDWRDSHLGNEPYLRVKNTKEPTFLYAMPFDRNLVFLEET 301

Query: 305 DFPRRPAGDAVHSSEKEDGGQVAAFGDKGEENYRRGEMCDSDGG---CSAEDAAGSGGGR 364
               RP    +   ++    ++   G K        +   + GG      ++    GG  
Sbjct: 302 SLVSRPMLSYM-EVKRRMVARLRHLGIKVRSVLEEEKCVITMGGPLPRIPQNVMAIGG-- 361

Query: 365 WGGGDGEPASGYAVVKGMGAAGRIARVAAEGVGGGGGRMIRGRALEGRVWEGVWPMEERN 424
              G   P+SGY V + M  A  +A    E +  G  RMIRG  L  RVW G+WP + R 
Sbjct: 362 -TSGIVHPSSGYMVARSMALAPVLAEAIVESL--GSTRMIRGSQLYHRVWNGLWPSDRRR 421

Query: 425 VREFYCFGMEILVRLDLDGIRGFFNAFFDLEPSYWEGLLSSRLSLLELGMLSLSLYQHAS 471
           VRE YCFGME L++LDL+G R  F+AFFD++P YW G LSSRLS+ EL +LSL L+ HAS
Sbjct: 422 VRECYCFGMETLLKLDLEGTRRLFDAFFDVDPKYWHGFLSSRLSVKELAVLSLYLFGHAS 481

BLAST of CsaV3_4G000740 vs. ExPASy Swiss-Prot
Match: Q9M424 (Neoxanthin synthase, chloroplastic OS=Solanum tuberosum OX=4113 GN=NXS PE=1 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 1.4e-89
Identity = 193/454 (42.51%), Postives = 269/454 (59.25%), Query Frame = 0

Query: 39  FDNFLDLKPESKPQPLKLDLPLFHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCV 98
           F +FLDL P SKP+   +++ L  P   R++FDV+++GAGP GLR+AE +  +GIKVCCV
Sbjct: 51  FCSFLDLAPISKPESFDVNISLVDPNSGRAQFDVIIIGAGPAGLRLAEHVSKYGIKVCCV 110

Query: 99  DPSPLSPWSNNYGVWVDEFEDLGLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLK 158
           DPSPLS W NNYGVWVDEFE+LGLEDCL   WPM  V+++D K KYL RPY +VSRKKLK
Sbjct: 111 DPSPLSMWPNNYGVWVDEFENLGLEDCLDHKWPMTCVHINDHKTKYLGRPYGRVSRKKLK 170

Query: 159 MKLMEECISKGVKFHKAKVWEINHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTKSRPP 218
           ++L+  C+   VKF+KAKVW++ H++FESS+ C+DG +I+ +LV+DASGF S F +   P
Sbjct: 171 LRLLNSCVENRVKFYKAKVWKVEHEEFESSIVCDDGKKIRGSLVVDASGFASDFIEYDKP 230

Query: 219 PIRSKQNGSNGL---ERHSF--EQRTIPKTRQQKIPNIPLRN-----------ALRFKPN 278
                Q     L   + H F  ++  +   R   + N P              A+ F  N
Sbjct: 231 RNHGYQIAHGVLVEVDNHPFDLDKMVLMDWRDSHLGNEPYLRVNNAKEPTFLYAMPFDRN 290

Query: 279 ---LSRRDFPRRPAGDAVHSSEKEDGGQVAAFGDKGEENYRRGEMCDSDGG---CSAEDA 338
              L       RP    +   ++    ++   G K        +     GG      ++ 
Sbjct: 291 LVFLEETSLVSRPVLSYM-EVKRRMVARLRHLGIKVRSVIEEEKCVIPMGGPLPRIPQNV 350

Query: 339 AGSGGGRWGGGDGEPASGYAVVKGMGAAGRIARVAAEGVGGGGGRMIRGRALEGRVWEGV 398
              GG     G   P++GY V + M  A  +A    +G+  G  RMIRG  L  RVW G+
Sbjct: 351 MAIGG---NSGIVHPSTGYMVARSMALAPVLAEAIVKGL--GSTRMIRGSQLYHRVWNGL 410

Query: 399 WPMEERNVREFYCFGMEILVRLDLDGIRGFFNAFFDLEPSYWEGLLSSRLSLLELGMLSL 458
           WP++ R + E Y FGME L++LDL G R  F+AFFDL+P YW+G LSSRLS+ EL +LSL
Sbjct: 411 WPLDRRCIGECYSFGMETLLKLDLKGTRRLFDAFFDLDPKYWQGFLSSRLSVKELAILSL 470

Query: 459 SLYQHASFRSKLDILTKSPLPLLQMMANLTLHAL 471
            L+ H S  ++LDI+TK P+PL++++ NL + +L
Sbjct: 471 CLFGHGSNLTRLDIVTKCPVPLVRLIGNLAIESL 498

BLAST of CsaV3_4G000740 vs. ExPASy Swiss-Prot
Match: Q38933 (Lycopene beta cyclase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LCY1 PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 4.3e-70
Identity = 173/450 (38.44%), Postives = 248/450 (55.11%), Query Frame = 0

Query: 43  LDLKPESKPQPLKLDLPLFHPYYDRSR---FDVVVVGAGPIGLRVAEQLGGFGIKVCCVD 102
           LDL PE+K + L  +LPL    YD S+    D+ +VG GP GL VA+Q+   G+ VC +D
Sbjct: 57  LDLVPETKKENLDFELPL----YDTSKSQVVDLAIVGGGPAGLAVAQQVSEAGLSVCSID 116

Query: 103 PSPLSPWSNNYGVWVDEFEDLGLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKM 162
           PSP   W NNYGVWVDEFE + L DCL  TW  A VY+ +   K L RPY +V+RK+LK 
Sbjct: 117 PSPKLIWPNNYGVWVDEFEAMDLLDCLDTTWSGAVVYVDEGVKKDLSRPYGRVNRKQLKS 176

Query: 163 KLMEECISKGVKFHKAKVWEINHQQFESSVSCNDGTEIKSNLVIDASGFTS---KFTKSR 222
           K++++CI+ GVKFH++KV  + H++  S+V C+DG +I++++V+DA+GF+    ++ K  
Sbjct: 177 KMLQKCITNGVKFHQSKVTNVVHEEANSTVVCSDGVKIQASVVLDATGFSRCLVQYDKPY 236

Query: 223 PPPIRSKQNGSNGLERHSFEQRTIP---------------KTRQQKIPNIPLRNALRFKP 282
            P  +        ++ H F+   +                K R  KIP      A+ F  
Sbjct: 237 NPGYQVAYGIVAEVDGHPFDVDKMVFMDWRDKHLDSYPELKERNSKIPT--FLYAMPFSS 296

Query: 283 N---LSRRDFPRRPAGDAVHSSEKEDGGQVAAFG---DKGEENYRRGEMCDSDGGCSAED 342
           N   L       RP G  +   ++    ++   G    + EE+ R             + 
Sbjct: 297 NRIFLEETSLVARP-GLRMEDIQERMAARLKHLGINVKRIEEDERCVIPMGGPLPVLPQR 356

Query: 343 AAGSGGGRWGGGDGEPASGYAVVKGMGAAGRIARVAAEGVGGGGGRMIRGRALEGRVWEG 402
             G GG     G   P++GY V + + AA  +A      +G      +RG  L   VW  
Sbjct: 357 VVGIGG---TAGMVHPSTGYMVARTLAAAPIVANAIVRYLGSPSSNSLRGDQLSAEVWRD 416

Query: 403 VWPMEERNVREFYCFGMEILVRLDLDGIRGFFNAFFDLEPSYWEGLLSSRLSLLELGMLS 462
           +WP+E R  REF+CFGM+IL++LDLD  R FF+AFFDL+P YW G LSSRL L EL +  
Sbjct: 417 LWPIERRRQREFFCFGMDILLKLDLDATRRFFDAFFDLQPHYWHGFLSSRLFLPELLVFG 476

Query: 463 LSLYQHASFRSKLDILTKSPLPLLQMMANL 466
           LSL+ HAS  S+L+I+TK  +PL +M+ NL
Sbjct: 477 LSLFSHASNTSRLEIMTKGTVPLAKMINNL 496

BLAST of CsaV3_4G000740 vs. ExPASy TrEMBL
Match: A0A0A0KY78 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G001730 PE=3 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 1.7e-194
Identity = 378/495 (76.36%), Postives = 394/495 (79.60%), Query Frame = 0

Query: 1   MANSQILLSPFSCLPRLPSYPIQSPSKNRFQIHSTFAKFDNFLDLKPESKPQPLKLDLPL 60
           MANSQILLSPFSCLPRLPSYPIQSPSKNRFQIHSTFAKFDNFLDLKPESKPQPLKLDLPL
Sbjct: 1   MANSQILLSPFSCLPRLPSYPIQSPSKNRFQIHSTFAKFDNFLDLKPESKPQPLKLDLPL 60

Query: 61  FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL 120
           FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL
Sbjct: 61  FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL 120

Query: 121 GLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEECISKGVKFHKAKVWEI 180
           GLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEECISKGVKFHKAKVWEI
Sbjct: 121 GLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEECISKGVKFHKAKVWEI 180

Query: 181 NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTK-SRPPPIRSKQNGSNGL----ERHSF 240
           NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTK S   P       ++G+    + H F
Sbjct: 181 NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTKYSESNPRNCAFQIAHGILAEVDHHPF 240

Query: 241 EQRTI-------------PKTRQ--QKIPN----IPLRNALRFKPNLSRRDFPRRPAGDA 300
           +   +             P  RQ  +K P     +P  + L F    S    P  P    
Sbjct: 241 DLNKMVLMDWRDTHLNNEPYLRQDNKKFPTFLYAMPFDSNLIFLEETSLAGRPAMP---- 300

Query: 301 VHSSEKEDGGQVAAFGDKGEENYRRGEMCDSDGGCSAEDAAGSGGGRWGGGDGEPASGYA 360
             + +K    ++   G K +      +     GG   +      G     G   PASGYA
Sbjct: 301 YTAVKKRMAARLRHLGIKVKRIIEEEKCVIPMGGALPKMPQAVVGVGGVAGMVNPASGYA 360

Query: 361 VVKGMGAAGRIARVAAEGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILV 420
           VVKGMGAAGRIARVAAEGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILV
Sbjct: 361 VVKGMGAAGRIARVAAEGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILV 420

Query: 421 RLDLDGIRGFFNAFFDLEPSYWEGLLSSRLSLLELGMLSLSLYQHASFRSKLDILTKSPL 472
           RLDLDGIRGFFNAFFDLEPSYWEGLLSSRLSLLELGMLSLSLYQHASFRSKLDILTKSPL
Sbjct: 421 RLDLDGIRGFFNAFFDLEPSYWEGLLSSRLSLLELGMLSLSLYQHASFRSKLDILTKSPL 480

BLAST of CsaV3_4G000740 vs. ExPASy TrEMBL
Match: A0A1S3BZM2 (capsanthin/capsorubin synthase, chromoplastic-like OS=Cucumis melo OX=3656 GN=LOC103494777 PE=3 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 8.6e-183
Identity = 360/492 (73.17%), Postives = 377/492 (76.63%), Query Frame = 0

Query: 1   MANSQILLSPFSCLPRLPSYPIQSPSKNRFQIHSTFAKFDNFLDLKPESKPQPLKLDLPL 60
           MANS+ LLSPF  LP+LPS PIQSPSKN  QIHSTFAKFDNFLDLKPESKP+PLKLDLPL
Sbjct: 1   MANSRFLLSPFFSLPQLPSNPIQSPSKNHLQIHSTFAKFDNFLDLKPESKPKPLKLDLPL 60

Query: 61  FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL 120
           FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL
Sbjct: 61  FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL 120

Query: 121 GLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEECISKGVKFHKAKVWEI 180
           GLEDCLHKTWPMAS+YLSDEKVKYLDRPYAQVSRKKLKMKLMEECI KGVKFHKAKVWEI
Sbjct: 121 GLEDCLHKTWPMASLYLSDEKVKYLDRPYAQVSRKKLKMKLMEECIRKGVKFHKAKVWEI 180

Query: 181 NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTK-SRPPPIRSKQNGSNGL----ERHSF 240
           NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTK S   P       ++G+    + H F
Sbjct: 181 NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTKYSDSNPRNCAFQIAHGILAEVDHHPF 240

Query: 241 EQRTI-------------PKTRQQKIPNIPLRNALRFKPN---LSRRDFPRRPAGDAVHS 300
           +   +             P  RQ          A+ F  N   L       RPA      
Sbjct: 241 DLNKMVLMDWRDTHLNNEPYLRQHNKEFPTFLYAMPFDSNLIFLEETSLVGRPALPYT-V 300

Query: 301 SEKEDGGQVAAFGDKGEENYRRGEMCDSDGGCSAEDAAGSGGGRWGGGDGEPASGYAVVK 360
            +K    ++   G K +      +     GG   E      G     G   PASGYAVVK
Sbjct: 301 VKKRMAARLRHLGIKVKRILEEEKCVIPMGGALPEMPQAVVGVGGVAGMVNPASGYAVVK 360

Query: 361 GMGAAGRIARVAAEGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILVRLD 420
           GM AAGRIARVA EGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEIL+RLD
Sbjct: 361 GMAAAGRIARVAGEGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILMRLD 420

Query: 421 LDGIRGFFNAFFDLEPSYWEGLLSSRLSLLELGMLSLSLYQHASFRSKLDILTKSPLPLL 472
           LDGIRGFFNAFFDLEP YWEG LSSRLSLLEL MLSLSLYQHASFRSKLDILTKSPLPLL
Sbjct: 421 LDGIRGFFNAFFDLEPFYWEGFLSSRLSLLELAMLSLSLYQHASFRSKLDILTKSPLPLL 480

BLAST of CsaV3_4G000740 vs. ExPASy TrEMBL
Match: A0A5D3E1M5 (Capsanthin/capsorubin synthase, chromoplastic-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001010 PE=3 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 2.1e-157
Identity = 325/480 (67.71%), Postives = 346/480 (72.08%), Query Frame = 0

Query: 1   MANSQILLSPFSCLPRLPSYPIQSPSKNRFQIHSTFAKFDNFLDLKPESKPQPLKLDLPL 60
           MANS+ LLSPF  LP+LPS PIQSPSKN  QIHSTFAKFDNFLDLKPESKP+PLKLDLPL
Sbjct: 1   MANSRFLLSPFFSLPQLPSNPIQSPSKNHLQIHSTFAKFDNFLDLKPESKPKPLKLDLPL 60

Query: 61  FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL 120
           FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL
Sbjct: 61  FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL 120

Query: 121 GLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEECISKGVKFHKAKVWEI 180
           GLEDCLHKTWPMAS+YLSDEKVKYLDRPYAQVSRKKLKMKLMEECI KGVKFHKAKVWEI
Sbjct: 121 GLEDCLHKTWPMASLYLSDEKVKYLDRPYAQVSRKKLKMKLMEECIRKGVKFHKAKVWEI 180

Query: 181 NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTK-SRPPPIRSKQNGSNGL----ERHSF 240
           NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTK S   P       ++G+    + H F
Sbjct: 181 NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTKYSDSNPRNCAFQIAHGILAEVDHHPF 240

Query: 241 E--QRTIPKTRQQKIPNIPL--RNALRFKPNLSRRDFPRRPAGDAVHSSEKEDGGQVAAF 300
           +  +  +   R   + N P   ++   F   L    F      + +   E    G+ A  
Sbjct: 241 DLNKMVLMDWRDTHLNNEPYLRQHNKEFPTFLYAMPFD----SNLIFLEETSLVGRPAL- 300

Query: 301 GDKGEENYRRGEMCDSDGGCSAEDAAGSGGGRWGGGDGEPASGYAVVKGMGAAGRIARVA 360
                                 +    +     G            V  MG A      A
Sbjct: 301 -----------------PYTVVKKRMAARLRHLGIKVKRILEEEKCVIPMGGALPEMPQA 360

Query: 361 AEGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILVRLDLDGIRGFFNAFF 420
             GVGG  G +    A   RVWEGVWPMEERNVREFYCFGMEIL+RLDLDGIRGFFNAFF
Sbjct: 361 VVGVGGVAGMV--NPASGXRVWEGVWPMEERNVREFYCFGMEILMRLDLDGIRGFFNAFF 420

Query: 421 DLEPSYWEGLLSSRLSLLELGMLSLSLYQHASFRSKLDILTKSPLPLLQMMANLTLHALG 472
           DLEP YWEG LSSRLSLLEL MLSLSLYQHASFRSKLDILTKSPLPLLQMMANLTL ALG
Sbjct: 421 DLEPFYWEGFLSSRLSLLELAMLSLSLYQHASFRSKLDILTKSPLPLLQMMANLTLQALG 456

BLAST of CsaV3_4G000740 vs. ExPASy TrEMBL
Match: A0A6J1I6P6 (capsanthin/capsorubin synthase, chromoplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111471649 PE=3 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 7.9e-144
Identity = 301/494 (60.93%), Postives = 343/494 (69.43%), Query Frame = 0

Query: 1   MANSQILLSPFSCLPRLPSYPIQSPSKNRFQIHSTFAKFDNFLDLKPESKPQPLKLDLPL 60
           MA S  LLSPFS LP +PS PI SP +  FQ H T  KF NFLDLKPESKPQPLK DLPL
Sbjct: 1   MAISHALLSPFS-LPHIPSSPIHSPFRTHFQPHYTLRKFANFLDLKPESKPQPLKFDLPL 60

Query: 61  FHPYYDR-SRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFED 120
           FHPY DR  RFD VVVGAGP GLR+AEQL  +GIKVCCVDPSPLSPW NNYGVWVDEF+ 
Sbjct: 61  FHPYSDRPRRFDAVVVGAGPAGLRLAEQLSAYGIKVCCVDPSPLSPWPNNYGVWVDEFQA 120

Query: 121 LGLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEECISKGVKFHKAKVWE 180
           LG +DC HKTWPMASVYL+DEKVK+LDRPYAQVSRKKLKMKLMEEC +KGVKFHKAKVWE
Sbjct: 121 LGFQDCFHKTWPMASVYLNDEKVKFLDRPYAQVSRKKLKMKLMEECATKGVKFHKAKVWE 180

Query: 181 INHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTK-SRPPPIRSKQNGSNGL----ERHS 240
           I HQ+FESSV+C+DGTEIK+NLVIDASGF S+FT+ S+  P       ++G+    E+H 
Sbjct: 181 IKHQEFESSVACDDGTEIKANLVIDASGFASRFTEYSKSKPRNCGFQIAHGILAEVEQHP 240

Query: 241 FEQRTI-------------PKTRQQKIPNIPLRNALRFKPN---LSRRDFPRRPAGDAVH 300
           F+   +             P  RQ    +     A+ F PN   L       RPA     
Sbjct: 241 FDLNKMVLMDWRDTHLGNEPYLRQDNKKSPTFLYAMPFDPNLIFLEETSLVGRPALPYA- 300

Query: 301 SSEKEDGGQVAAFGDKGEENYRRGEMCDSDGGC--SAEDAAGSGGGRWGGGDGEPASGYA 360
             ++    ++   G K +      +     GG       A  + GG    G   PASGY+
Sbjct: 301 VVKRRMAARLRHLGIKVKRILEEEKCVIPMGGSLPKMPQAVVAAGG--VAGMVHPASGYS 360

Query: 361 VVKGMGAAGRIARVAAEGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILV 420
           VV GM AA  +AR AAEG+ G GGRMIRGRAL+GRVWEGVWP E R VREFYCFGME LV
Sbjct: 361 VVLGMAAAPGVARRAAEGL-GSGGRMIRGRALQGRVWEGVWPAERRRVREFYCFGMETLV 420

Query: 421 RLDLDGIRGFFNAFFDLEPSYWEGLLSSRLSLLELGMLSLSLYQHASFRSKLDILTKSPL 471
           RLDL+G+R FF+AFFDLE  YWEG LSSRLS+ ELGML L+L+ HAS  SKLDILTK PL
Sbjct: 421 RLDLEGLRRFFDAFFDLEGFYWEGFLSSRLSIRELGMLGLALFGHASPMSKLDILTKCPL 480

BLAST of CsaV3_4G000740 vs. ExPASy TrEMBL
Match: A0A6J1ENN2 (capsanthin/capsorubin synthase, chromoplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111434203 PE=3 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 2.2e-141
Identity = 298/494 (60.32%), Postives = 339/494 (68.62%), Query Frame = 0

Query: 1   MANSQILLSPFSCLPRLPSYPIQSPSKNRFQIHSTFAKFDNFLDLKPESKPQPLKLDLPL 60
           MA S  LLSPFS LP +PS PI SP +  FQ H T  +F NFLDLKPESKPQPLK DLPL
Sbjct: 1   MAISHALLSPFS-LPDIPSSPIHSPFRTHFQPHCTLRRFGNFLDLKPESKPQPLKFDLPL 60

Query: 61  FHPYYDR-SRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFED 120
           FHPY DR  RFDVVVVGAGP GLR+AEQL  +GIKVCCVDPSPLSPW NNYGVWVDEF+ 
Sbjct: 61  FHPYSDRPRRFDVVVVGAGPAGLRLAEQLSAYGIKVCCVDPSPLSPWPNNYGVWVDEFQA 120

Query: 121 LGLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEECISKGVKFHKAKVWE 180
           LG +DC HKTWPMASVYL+DEKVK+LDRPYAQVSRKKLKMKLMEEC +KGVKFHKAKVWE
Sbjct: 121 LGFQDCFHKTWPMASVYLNDEKVKFLDRPYAQVSRKKLKMKLMEECATKGVKFHKAKVWE 180

Query: 181 INHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTK-SRPPPIRSKQNGSNGL----ERHS 240
           I HQ+FESSV+C+DGTEIK+NLVID SGF S+FT+ S   P       ++G+    E+H 
Sbjct: 181 IKHQEFESSVACDDGTEIKANLVIDTSGFASRFTEYSNSKPRNCGFQIAHGILAEVEQHP 240

Query: 241 FEQRTI-------------PKTRQQKIPNIPLRNALRFKPN---LSRRDFPRRPAGDAVH 300
           F+   +             P  RQ    +     A+ F  N   L       RPA     
Sbjct: 241 FDLNKMVLMDWRDTHLGNEPYLRQDNKKSPTFLYAMPFDSNLIFLEETSLVGRPALPYA- 300

Query: 301 SSEKEDGGQVAAFGDKGEENYRRGEMCDSDGGC--SAEDAAGSGGGRWGGGDGEPASGYA 360
             ++    ++   G K +      +     GG       A  + GG    G   PASGY+
Sbjct: 301 VVKRRMAARLRHLGIKVKRILEEEKCVIPMGGSLPKMPQAVVAAGG--VAGMVHPASGYS 360

Query: 361 VVKGMGAAGRIARVAAEGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILV 420
           VV GM AA  +AR AAEG+ G GGRMIRGRAL+GRVWEGVWP E R VREFYCFGME LV
Sbjct: 361 VVLGMAAAPGVARRAAEGL-GSGGRMIRGRALQGRVWEGVWPAERRRVREFYCFGMETLV 420

Query: 421 RLDLDGIRGFFNAFFDLEPSYWEGLLSSRLSLLELGMLSLSLYQHASFRSKLDILTKSPL 471
           RLDL+G+R FF+AFFDLE  YWEG LSSRLS+ ELGML L L+ HAS  SKLDILTK PL
Sbjct: 421 RLDLEGLRRFFDAFFDLEGFYWEGFLSSRLSIRELGMLGLLLFGHASPMSKLDILTKCPL 480

BLAST of CsaV3_4G000740 vs. TAIR 10
Match: AT3G10230.1 (lycopene cyclase )

HSP 1 Score: 266.9 bits (681), Expect = 3.0e-71
Identity = 173/450 (38.44%), Postives = 248/450 (55.11%), Query Frame = 0

Query: 43  LDLKPESKPQPLKLDLPLFHPYYDRSR---FDVVVVGAGPIGLRVAEQLGGFGIKVCCVD 102
           LDL PE+K + L  +LPL    YD S+    D+ +VG GP GL VA+Q+   G+ VC +D
Sbjct: 57  LDLVPETKKENLDFELPL----YDTSKSQVVDLAIVGGGPAGLAVAQQVSEAGLSVCSID 116

Query: 103 PSPLSPWSNNYGVWVDEFEDLGLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKM 162
           PSP   W NNYGVWVDEFE + L DCL  TW  A VY+ +   K L RPY +V+RK+LK 
Sbjct: 117 PSPKLIWPNNYGVWVDEFEAMDLLDCLDTTWSGAVVYVDEGVKKDLSRPYGRVNRKQLKS 176

Query: 163 KLMEECISKGVKFHKAKVWEINHQQFESSVSCNDGTEIKSNLVIDASGFTS---KFTKSR 222
           K++++CI+ GVKFH++KV  + H++  S+V C+DG +I++++V+DA+GF+    ++ K  
Sbjct: 177 KMLQKCITNGVKFHQSKVTNVVHEEANSTVVCSDGVKIQASVVLDATGFSRCLVQYDKPY 236

Query: 223 PPPIRSKQNGSNGLERHSFEQRTIP---------------KTRQQKIPNIPLRNALRFKP 282
            P  +        ++ H F+   +                K R  KIP      A+ F  
Sbjct: 237 NPGYQVAYGIVAEVDGHPFDVDKMVFMDWRDKHLDSYPELKERNSKIPT--FLYAMPFSS 296

Query: 283 N---LSRRDFPRRPAGDAVHSSEKEDGGQVAAFG---DKGEENYRRGEMCDSDGGCSAED 342
           N   L       RP G  +   ++    ++   G    + EE+ R             + 
Sbjct: 297 NRIFLEETSLVARP-GLRMEDIQERMAARLKHLGINVKRIEEDERCVIPMGGPLPVLPQR 356

Query: 343 AAGSGGGRWGGGDGEPASGYAVVKGMGAAGRIARVAAEGVGGGGGRMIRGRALEGRVWEG 402
             G GG     G   P++GY V + + AA  +A      +G      +RG  L   VW  
Sbjct: 357 VVGIGG---TAGMVHPSTGYMVARTLAAAPIVANAIVRYLGSPSSNSLRGDQLSAEVWRD 416

Query: 403 VWPMEERNVREFYCFGMEILVRLDLDGIRGFFNAFFDLEPSYWEGLLSSRLSLLELGMLS 462
           +WP+E R  REF+CFGM+IL++LDLD  R FF+AFFDL+P YW G LSSRL L EL +  
Sbjct: 417 LWPIERRRQREFFCFGMDILLKLDLDATRRFFDAFFDLQPHYWHGFLSSRLFLPELLVFG 476

Query: 463 LSLYQHASFRSKLDILTKSPLPLLQMMANL 466
           LSL+ HAS  S+L+I+TK  +PL +M+ NL
Sbjct: 477 LSLFSHASNTSRLEIMTKGTVPLAKMINNL 496

BLAST of CsaV3_4G000740 vs. TAIR 10
Match: AT3G10230.2 (lycopene cyclase )

HSP 1 Score: 184.9 bits (468), Expect = 1.5e-46
Identity = 131/370 (35.41%), Postives = 196/370 (52.97%), Query Frame = 0

Query: 120 LGLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEECISKGVKFHKAKVWE 179
           + L DCL  TW  A VY+ +   K L RPY +V+RK+LK K++++CI+ GVKFH++KV  
Sbjct: 1   MDLLDCLDTTWSGAVVYVDEGVKKDLSRPYGRVNRKQLKSKMLQKCITNGVKFHQSKVTN 60

Query: 180 INHQQFESSVSCNDGTEIKSNLVIDASGFTS---KFTKSRPPPIRSKQNGSNGLERHSFE 239
           + H++  S+V C+DG +I++++V+DA+GF+    ++ K   P  +        ++ H F+
Sbjct: 61  VVHEEANSTVVCSDGVKIQASVVLDATGFSRCLVQYDKPYNPGYQVAYGIVAEVDGHPFD 120

Query: 240 QRTIP---------------KTRQQKIPNIPLRNALRFKPN---LSRRDFPRRPAGDAVH 299
              +                K R  KIP      A+ F  N   L       RP G  + 
Sbjct: 121 VDKMVFMDWRDKHLDSYPELKERNSKIPT--FLYAMPFSSNRIFLEETSLVARP-GLRME 180

Query: 300 SSEKEDGGQVAAFG---DKGEENYRRGEMCDSDGGCSAEDAAGSGGGRWGGGDGEPASGY 359
             ++    ++   G    + EE+ R             +   G GG     G   P++GY
Sbjct: 181 DIQERMAARLKHLGINVKRIEEDERCVIPMGGPLPVLPQRVVGIGG---TAGMVHPSTGY 240

Query: 360 AVVKGMGAAGRIARVAAEGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEIL 419
            V + + AA  +A      +G      +RG  L   VW  +WP+E R  REF+CFGM+IL
Sbjct: 241 MVARTLAAAPIVANAIVRYLGSPSSNSLRGDQLSAEVWRDLWPIERRRQREFFCFGMDIL 300

Query: 420 VRLDLDGIRGFFNAFFDLEPSYWEGLLSSRLSLLELGMLSLSLYQHASFRSKLDILTKSP 466
           ++LDLD  R FF+AFFDL+P YW G LSSRL L EL +  LSL+ HAS  S+L+I+TK  
Sbjct: 301 LKLDLDATRRFFDAFFDLQPHYWHGFLSSRLFLPELLVFGLSLFSHASNTSRLEIMTKGT 360

BLAST of CsaV3_4G000740 vs. TAIR 10
Match: AT5G57030.1 (Lycopene beta/epsilon cyclase protein )

HSP 1 Score: 115.9 bits (289), Expect = 8.6e-26
Identity = 109/391 (27.88%), Postives = 171/391 (43.73%), Query Frame = 0

Query: 71  DVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDLGLEDCLHKTW 130
           D+VV+G GP GL +A +    G+KV  + P    P++NNYGVW DEF DLGL+ C+   W
Sbjct: 110 DLVVIGCGPAGLALAAESAKLGLKVGLIGPD--LPFTNNYGVWEDEFNDLGLQKCIEHVW 169

Query: 131 PMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEECISKGVKFHKAKVWEINH-QQFESSV 190
               VYL D+K   + R Y +VSR+ L  +L+  C+  GV +  +KV  I         V
Sbjct: 170 RETIVYLDDDKPITIGRAYGRVSRRLLHEELLRRCVESGVSYLSSKVDSITEASDGLRLV 229

Query: 191 SCNDGTEIKSNLVIDASGFTS-KFTKSRPPPIRSKQNGSNGLERHSFEQRTIPKTRQQKI 250
           +C+D   I   L   ASG  S K  +      R     + G+E    E    P    Q +
Sbjct: 230 ACDDNNVIPCRLATVASGAASGKLLQYEVGGPRVCVQTAYGVE---VEVENSPYDPDQMV 289

Query: 251 PNIPLRNALRFKPNLSRRDFP----------RRPAGDAVHSSEKEDGG----------QV 310
             +  R+    K      ++P           R   +    + K+             ++
Sbjct: 290 -FMDYRDYTNEKVRSLEAEYPTFLYAMPMTKSRLFFEETCLASKDVMPFDLLKTKLMLRL 349

Query: 311 AAFGDKGEENYRRGEMCDSDGGCSAEDAAGSGGGRWGGGDGEPASGYAVVKGMGAAGRIA 370
              G +  + Y         GG        +           PA+GY+VV+ +  A + A
Sbjct: 350 DTLGIRILKTYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPKYA 409

Query: 371 RVAAEGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILVRLDLDGIRGFFN 430
            V AE +     + I    +  + W+ +WP E +  R F+ FG+ ++V+ D +GIR FF 
Sbjct: 410 SVIAEILREETTKQINSN-ISRQAWDTLWPPERKRQRAFFLFGLALIVQFDTEGIRSFFR 469

Query: 431 AFFDLEPSYWEGLLSSRLSLLELGMLSLSLY 440
            FF L    W+G L S L+  +L + +L ++
Sbjct: 470 TFFRLPKWMWQGFLGSTLTSGDLVLFALYMF 493