CsaV3_3G041400 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_3G041400
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	Ribonuclease MC-like
Location	chr3: 33747582 .. 33748669 (-)
RNA-Seq Expression	CsaV3_3G041400
Synteny	CsaV3_3G041400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTAATAAAATGGTGGTAGCAATGGCAGGGGGAAGGAGATCAGATATTAATATTGTGTTATTGTTCGTGGTTACCTTAAGCATCAGCTTGTTTCCTATTATGAAATCACAACAATTTGATAAATTTTACTTTGTTCAACAATGGCCACCAGCTGTTTGCGATGGACGTACAGGAAAATGTGTAGGAAAAGGGATGTACTACTTTACAATTCATGGTGTGTGGCCTCAGAAAGGAGGAAAATCTGTAATTAATTGCCCTGGCACCCAATTTGATTTCAATAAGGTATTCTATTTTAGAATTCTCAAATTCACTCTATAAATTTTCCTCTATCTACATATATATATTATTTAAATTAACTTTGAATAAAAAGAACGATTTGAAAATAAATACATAGTTTAGTCTCAACACGACTCTTTTTAACATTACGTGGGAATGAGTTTCAGATTTCGAGTTTAGCAAACACTCTACATCAAATTATGAAGGATGTGATAAACGCAGACGACCAATTTTTATGGTCTCACGAGTGGAACAAACATGGAGTTTGCTCAGAAAGCAGGTACTCAATGAAACAATACTTCCAAATGGCGATAAATATGAAGTACAAGATCAATGTTCTTAGTGCCCTGAGGATGGGTGGGATAACCCCAAACAACCACTTGAAGGCCAAACAAAGAGTAGAGGGGGCGATGTTCACTGCATATAACGCATATCCTCTTCTTCGATGTAAAAAAGATTCATCTGGCCAATCCCTATTGACGGAGGTTGTTATGTGCTTTGACAATGATGGTGTCACCCTTCTAAACTGCACTACAACGAAATCCAATTGTGATGCAGACGTTCTCTTTTGATAACATATGTAAACTCTTCAATTCTCATGCATGCCAATAATAATGTTATTAGTTAACAATCCATGCATGTGCTTAATTCCATGCATAAACATAAGAAATAAAATATATATGTGTCATGTGGTTATCTATCTATAATTAAAATAAGTTGGTGCTATGCTTCGTCACCAATATTTGTCTCCAAGTTTATATAATATACCGTGTGTTTAAAGTTGTTGTCTTCTGTAATTTAAAGATACAGG

mRNA sequence

ATGGTGGTAGCAATGGCAGGGGGAAGGAGATCAGATATTAATATTGTGTTATTGTTCGTGGTTACCTTAAGCATCAGCTTGTTTCCTATTATGAAATCACAACAATTTGATAAATTTTACTTTGTTCAACAATGGCCACCAGCTGTTTGCGATGGACGTACAGGAAAATGTGTAGGAAAAGGGATGTACTACTTTACAATTCATGGTGTGTGGCCTCAGAAAGGAGGAAAATCTGTAATTAATTGCCCTGGCACCCAATTTGATTTCAATAAGATTTCGAGTTTAGCAAACACTCTACATCAAATTATGAAGGATGTGATAAACGCAGACGACCAATTTTTATGGTCTCACGAGTGGAACAAACATGGAGTTTGCTCAGAAAGCAGGTACTCAATGAAACAATACTTCCAAATGGCGATAAATATGAAGTACAAGATCAATGTTCTTAGTGCCCTGAGGATGGGTGGGATAACCCCAAACAACCACTTGAAGGCCAAACAAAGAGTAGAGGGGGCGATGTTCACTGCATATAACGCATATCCTCTTCTTCGATGTAAAAAAGATTCATCTGGCCAATCCCTATTGACGGAGGTTGTTATGTGCTTTGACAATGATGGTGTCACCCTTCTAAACTGCACTACAACGAAATCCAATTGTGATGCAGACGTTCTCTTTTGA

Coding sequence (CDS)

ATGGTGGTAGCAATGGCAGGGGGAAGGAGATCAGATATTAATATTGTGTTATTGTTCGTGGTTACCTTAAGCATCAGCTTGTTTCCTATTATGAAATCACAACAATTTGATAAATTTTACTTTGTTCAACAATGGCCACCAGCTGTTTGCGATGGACGTACAGGAAAATGTGTAGGAAAAGGGATGTACTACTTTACAATTCATGGTGTGTGGCCTCAGAAAGGAGGAAAATCTGTAATTAATTGCCCTGGCACCCAATTTGATTTCAATAAGATTTCGAGTTTAGCAAACACTCTACATCAAATTATGAAGGATGTGATAAACGCAGACGACCAATTTTTATGGTCTCACGAGTGGAACAAACATGGAGTTTGCTCAGAAAGCAGGTACTCAATGAAACAATACTTCCAAATGGCGATAAATATGAAGTACAAGATCAATGTTCTTAGTGCCCTGAGGATGGGTGGGATAACCCCAAACAACCACTTGAAGGCCAAACAAAGAGTAGAGGGGGCGATGTTCACTGCATATAACGCATATCCTCTTCTTCGATGTAAAAAAGATTCATCTGGCCAATCCCTATTGACGGAGGTTGTTATGTGCTTTGACAATGATGGTGTCACCCTTCTAAACTGCACTACAACGAAATCCAATTGTGATGCAGACGTTCTCTTTTGA

Protein sequence

MVVAMAGGRRSDINIVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIHGVWPQKGGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLRCKKDSSGQSLLTEVVMCFDNDGVTLLNCTTTKSNCDADVLF*

Homology

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BLAST of CsaV3_3G041400 vs. NCBI nr
Match: XP_004147497.3 (ribonuclease MC [Cucumis sativus] >KAE8651218.1 hypothetical protein Csa_002248 [Cucumis sativus])

HSP 1 Score: 471.1 bits (1211), Expect = 5.4e-129
Identity = 225/225 (100.00%), Postives = 225/225 (100.00%), Query Frame = 0


Query: 1   MVVAMAGGRRSDINIVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGK 60
           MVVAMAGGRRSDINIVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGK
Sbjct: 1   MVVAMAGGRRSDINIVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGK 60

Query: 61  GMYYFTIHGVWPQKGGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWN 120
           GMYYFTIHGVWPQKGGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWN
Sbjct: 61  GMYYFTIHGVWPQKGGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWN 120

Query: 121 KHGVCSESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAY 180
           KHGVCSESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAY
Sbjct: 121 KHGVCSESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAY 180

Query: 181 PLLRCKKDSSGQSLLTEVVMCFDNDGVTLLNCTTTKSNCDADVLF 226
           PLLRCKKDSSGQSLLTEVVMCFDNDGVTLLNCTTTKSNCDADVLF
Sbjct: 181 PLLRCKKDSSGQSLLTEVVMCFDNDGVTLLNCTTTKSNCDADVLF 225

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BLAST of CsaV3_3G041400 vs. NCBI nr
Match: KAA0053765.1 (ribonuclease MC-like [Cucumis melo var. makuwa] >TYK25637.1 ribonuclease MC-like [Cucumis melo var. makuwa])

HSP 1 Score: 346.7 bits (888), Expect = 1.5e-91
Identity = 159/210 (75.71%), Postives = 178/210 (84.76%), Query Frame = 0


Query: 16  VLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIHGVWPQKG 75
           +LLFVVTLSISLFP MKSQ FD FYFVQQWPPAVC GR+G+CVG+G  YFTIHGVWPQKG
Sbjct: 1   MLLFVVTLSISLFPTMKSQIFDDFYFVQQWPPAVCAGRSGQCVGQGFRYFTIHGVWPQKG 60

Query: 76  GKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQY 135
           G+S++NCPGT+FD N+ISSL NTLHQ   DVIN  DQ  W HEWNKHG CSES+Y M  Y
Sbjct: 61  GQSIVNCPGTKFDVNQISSLTNTLHQTWPDVINGKDQTFWEHEWNKHGTCSESKYQMLPY 120

Query: 136 FQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLRCKKDSSGQSLL 195
           FQMAINMKYKI+VL A+R+GG+ PNNH KAKQRVE  +F AY+AYPLLRCKKDS+GQ LL
Sbjct: 121 FQMAINMKYKIDVLGAMRVGGVVPNNHKKAKQRVEVVIFNAYHAYPLLRCKKDSTGQYLL 180

Query: 196 TEVVMCFDNDGVTLLNCTTTKSNCDADVLF 226
           TEVVMCF +DGVTLLNCT +KS C AD LF
Sbjct: 181 TEVVMCFKDDGVTLLNCTRSKSTCGADFLF 210

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BLAST of CsaV3_3G041400 vs. NCBI nr
Match: KAA0053767.1 (ribonuclease MC-like [Cucumis melo var. makuwa] >TYK25635.1 ribonuclease MC-like [Cucumis melo var. makuwa])

HSP 1 Score: 345.1 bits (884), Expect = 4.5e-91
Identity = 158/210 (75.24%), Postives = 178/210 (84.76%), Query Frame = 0


Query: 16  VLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIHGVWPQKG 75
           +LLFVVTLSISLFP MKSQ FD FYFVQQWPPAVC GR+G+CVG+G  YFTIHGVWPQKG
Sbjct: 1   MLLFVVTLSISLFPTMKSQIFDDFYFVQQWPPAVCAGRSGQCVGQGFRYFTIHGVWPQKG 60

Query: 76  GKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQY 135
           G+S++NCPGT+FD N+ISSL NTLHQ   DVIN  DQ  W HEWNKHG CSES++ M  Y
Sbjct: 61  GQSIVNCPGTKFDVNQISSLTNTLHQTWPDVINGKDQTFWEHEWNKHGTCSESKFQMLPY 120

Query: 136 FQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLRCKKDSSGQSLL 195
           FQMAINMKYKI+VL A+R+GG+ PNNH KAKQRVE  +F AY+AYPLLRCKKDS+GQ LL
Sbjct: 121 FQMAINMKYKIDVLGAMRVGGVVPNNHKKAKQRVEVVIFNAYHAYPLLRCKKDSTGQYLL 180

Query: 196 TEVVMCFDNDGVTLLNCTTTKSNCDADVLF 226
           TEVVMCF +DGVTLLNCT +KS C AD LF
Sbjct: 181 TEVVMCFKDDGVTLLNCTRSKSTCGADFLF 210

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BLAST of CsaV3_3G041400 vs. NCBI nr
Match: XP_004147499.1 (ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [Cucumis sativus])

HSP 1 Score: 270.0 bits (689), Expect = 1.8e-68
Identity = 122/210 (58.10%), Postives = 159/210 (75.71%), Query Frame = 0


Query: 16  VLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIHGVWPQKG 75
           V+ FV  L+I LFPI+KSQ FD F+FVQQWPPAVC  ++G+CVG+G   FTIHG+WPQKG
Sbjct: 9   VVFFVFVLTI-LFPIVKSQTFDDFWFVQQWPPAVCTLQSGRCVGRGTRSFTIHGLWPQKG 68

Query: 76  GKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQY 135
           G+SV NC G QFDF KI+ L N L+ +  +V+  +++F W HEWNKHG+CSES++   +Y
Sbjct: 69  GRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKY 128

Query: 136 FQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLRCKKDSSGQSLL 195
           FQ AINM++ I++LS LR GG+ PN   KAKQRVE A+ + +   P+LRCKK S+GQ LL
Sbjct: 129 FQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLL 188

Query: 196 TEVVMCFDNDGVTLLNCTTTKSNCDADVLF 226
           TE+VMCFD+DGVTL+NC   +SNC    +F
Sbjct: 189 TEIVMCFDDDGVTLINCNKARSNCAGSFIF 217

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BLAST of CsaV3_3G041400 vs. NCBI nr
Match: XP_022155091.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 268.1 bits (684), Expect = 7.0e-68
Identity = 122/211 (57.82%), Postives = 161/211 (76.30%), Query Frame = 0


Query: 15  IVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIHGVWPQK 74
           IV +FV+ ++I L PI+KSQ FD F+FVQQWPPAVC  + G+CVG+G+  FTIHGVWPQK
Sbjct: 8   IVFMFVLAITIFL-PIVKSQTFDDFWFVQQWPPAVCSFQQGRCVGQGLRTFTIHGVWPQK 67

Query: 75  GGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQ 134
           GG SV+NCPGT FDF KISSL   L+++  +VI  D++F WSHEWNKHGVCSES+++MK 
Sbjct: 68  GGTSVVNCPGTPFDFTKISSLETDLNRVWPNVITGDNKFFWSHEWNKHGVCSESKFNMKA 127

Query: 135 YFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLRCKKDSSGQSL 194
           YFQMAINM+  I++++AL++GG+ PN   K+KQRV+ A+ T +   P+LRC+   S   L
Sbjct: 128 YFQMAINMRNSIDLMNALKVGGLAPNGLSKSKQRVQSALRTHFGKEPVLRCRGTGSNIRL 187

Query: 195 LTEVVMCFDNDGVTLLNCTTTKSNCDADVLF 226
           + E+VMCFD+DGVTL+NC    SNC    +F
Sbjct: 188 I-EIVMCFDDDGVTLINCNPVSSNCANSFIF 216

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BLAST of CsaV3_3G041400 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 6.0e-46
Identity = 85/193 (44.04%), Postives = 124/193 (64.25%), Query Frame = 0


Query: 36  FDKFYFVQQWPPAVCD-GRTGKCVGKGMYYFTIHGVWPQKGGKSVINCP-GTQFDFNKIS 95
           FD F+FVQQWPPAVC   ++G C G G+  FTIHG+WPQ  G S+ NCP G+ FD  KIS
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 96  SLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQYFQMAINMKYKINVLSALR 155
            L + L+ +  +V+ A++Q  WSHEW KHG CSES ++   YF++A++M+   +++ ALR
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALR 120

Query: 156 MGGITPNNHLKAKQRVEGAMFTAYNAYPLLRCKKDSSGQ-SLLTEVVMCFDNDGVTLLNC 215
                PN   K++Q ++G +   +  +P LRC+ D   + S L +VV CF  DG TL++C
Sbjct: 121 PHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLIDC 180

Query: 216 TTTKSNCDADVLF 226
             T+  C A+ +F
Sbjct: 181 --TRDTCGANFIF 191

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BLAST of CsaV3_3G041400 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 2.7e-30
Identity = 75/221 (33.94%), Postives = 113/221 (51.13%), Query Frame = 0


Query: 14  NIVLLFVVTLSISLFPI-MKSQQFDKFYFVQQWPPAVCDGRTGKCV---GKGMYYFTIHG 73
           ++ L+ ++ +  S+F     S+ FD FYFVQQWP + CD +   C    GK    F IHG
Sbjct: 7   SLCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHG 66

Query: 74  VWPQ-KGGKSVINCPGTQ-FDFNKISSLANTLHQIMKDVI--NADDQFLWSHEWNKHGVC 133
           +WP  K G    NC  ++ FD + IS L  ++ +    +   +   +  W HEW KHG C
Sbjct: 67  LWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTC 126

Query: 134 SESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLRC 193
           SES     +YFQ A+N+K K N+L AL   GI P+    + + +  ++  +    P + C
Sbjct: 127 SESVIDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVEC 186

Query: 194 KKDSSGQSLLTEVVMCFDNDGVTLLNCTT-TKSNCDADVLF 226
            +D SG S L +V +C D  G  L+ C       C A++ F
Sbjct: 187 NRDGSGNSQLYQVYLCVDRSGSGLIECPVFPHGKCGAEIEF 227

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BLAST of CsaV3_3G041400 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 2.3e-29
Identity = 75/216 (34.72%), Postives = 106/216 (49.07%), Query Frame = 0


Query: 18  LFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKC---VGKGMYYFTIHGVWPQ- 77
           +F++ L   L+    +Q FD FYFV QWP A CD R   C    GK    F IHG+WP  
Sbjct: 5   IFILALQ-QLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNY 64

Query: 78  KGGKSVINC-PGTQFDFNKISSLANTLHQIMKDVI--NADDQFLWSHEWNKHGVCSESRY 137
           K G    NC P ++FD  ++S L + L +    +   + D    W+HEW KHG C+ES  
Sbjct: 65  KTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESEL 124

Query: 138 SMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLRCKKDSS 197
               YF+  + +K K N+L AL   GI P++     + +E  +       P + C KDSS
Sbjct: 125 DQHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSS 184

Query: 198 GQSLLTEVVMCFDNDGVTLLNC-TTTKSNCDADVLF 226
             S L ++ +C D      +NC       CD+ V F
Sbjct: 185 HNSQLYQIYLCVDTSASKFINCPVMPHGRCDSRVQF 219

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BLAST of CsaV3_3G041400 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 123.2 bits (308), Expect = 3.6e-27
Identity = 69/205 (33.66%), Postives = 111/205 (54.15%), Query Frame = 0


Query: 16  VLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKC---VGKGMYYFTIHGVWP 75
           ++L ++T  +S+  +  ++ FD FYFVQQWP + CD +   C    GK    F IHG+WP
Sbjct: 11  LILLIITQCLSV--LNAAKDFDFFYFVQQWPGSYCDTKQSCCYPTTGKPAADFGIHGLWP 70

Query: 76  QKG-GKSVINC-PGTQFDFNKISSLANTLHQIMKDVI--NADDQFLWSHEWNKHGVCSES 135
               G    NC P + +D ++IS L +++ Q    +   +      WSHEW KHG C+ES
Sbjct: 71  NNNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTCAES 130

Query: 136 RYSMKQ-YFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLRCKK 195
             + +  YF+ A+++K +I++LS L+   I P+        +  A+ +A    P ++C  
Sbjct: 131 VLTNQHAYFKKALDLKNQIDLLSILQGADIHPDGESYDLVNIRNAIKSAIGYTPWIQCNV 190

Query: 196 DSSGQSLLTEVVMCFDNDGVTLLNC 213
           D SG S L +V +C D  G +L+ C
Sbjct: 191 DQSGNSQLYQVYICVDGSGSSLIEC 213

[Close]

BLAST of CsaV3_3G041400 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 117.5 bits (293), Expect = 2.0e-25
Identity = 70/205 (34.15%), Postives = 102/205 (49.76%), Query Frame = 0


Query: 16  VLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKC---VGKGMYYFTIHGVWP 75
           +L+ ++ +   L   + SQ FD FYFVQQWP + CD R   C    GK    F+IHG+WP
Sbjct: 8   LLIKIIVVQCLLVLCVTSQDFDFFYFVQQWPASYCDTRRSCCYPTTGKPDEDFSIHGLWP 67

Query: 76  Q-KGGKSVINCP-GTQFDFNKISSLANTLHQIMKDVI--NADDQFLWSHEWNKHGVCSES 135
             K GK   NC   +  D ++ S L +T+ +    +   ++D    WSHEW KHG C  S
Sbjct: 68  NYKDGKWPQNCDRESSLDESEFSDLISTMEKNWPSLACPSSDGLKFWSHEWLKHGTC--S 127

Query: 136 RYSMKQYFQMAINMKYKINVLSALRMGGITP-NNHLKAKQRVEGAMFTAYNAYPLLRCKK 195
             +   YFQ A++ K K N+L  L   GI P N      + ++ A+       P + C  
Sbjct: 128 ALNQHAYFQTALDFKTKSNLLQNLNNAGIKPRNGDYYGVESIKKAIEKGVGHTPFIECNV 187

Query: 196 DSSGQSLLTEVVMCFDNDGVTLLNC 213
           DS G   L +V +C D+     ++C
Sbjct: 188 DSQGNHQLYQVYLCVDSSASKFIDC 210

[Close]

BLAST of CsaV3_3G041400 vs. ExPASy TrEMBL
Match: A0A0A0LHQ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G825060 PE=3 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.1e-127
Identity = 221/221 (100.00%), Postives = 221/221 (100.00%), Query Frame = 0


Query: 5   MAGGRRSDINIVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYY 64
           MAGGRRSDINIVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYY
Sbjct: 1   MAGGRRSDINIVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYY 60

Query: 65  FTIHGVWPQKGGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGV 124
           FTIHGVWPQKGGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGV
Sbjct: 61  FTIHGVWPQKGGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGV 120

Query: 125 CSESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLR 184
           CSESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLR
Sbjct: 121 CSESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLR 180

Query: 185 CKKDSSGQSLLTEVVMCFDNDGVTLLNCTTTKSNCDADVLF 226
           CKKDSSGQSLLTEVVMCFDNDGVTLLNCTTTKSNCDADVLF
Sbjct: 181 CKKDSSGQSLLTEVVMCFDNDGVTLLNCTTTKSNCDADVLF 221

[Close]

BLAST of CsaV3_3G041400 vs. ExPASy TrEMBL
Match: A0A5A7UJ53 (Ribonuclease MC-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007970 PE=3 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 7.5e-92
Identity = 159/210 (75.71%), Postives = 178/210 (84.76%), Query Frame = 0


Query: 16  VLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIHGVWPQKG 75
           +LLFVVTLSISLFP MKSQ FD FYFVQQWPPAVC GR+G+CVG+G  YFTIHGVWPQKG
Sbjct: 1   MLLFVVTLSISLFPTMKSQIFDDFYFVQQWPPAVCAGRSGQCVGQGFRYFTIHGVWPQKG 60

Query: 76  GKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQY 135
           G+S++NCPGT+FD N+ISSL NTLHQ   DVIN  DQ  W HEWNKHG CSES+Y M  Y
Sbjct: 61  GQSIVNCPGTKFDVNQISSLTNTLHQTWPDVINGKDQTFWEHEWNKHGTCSESKYQMLPY 120

Query: 136 FQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLRCKKDSSGQSLL 195
           FQMAINMKYKI+VL A+R+GG+ PNNH KAKQRVE  +F AY+AYPLLRCKKDS+GQ LL
Sbjct: 121 FQMAINMKYKIDVLGAMRVGGVVPNNHKKAKQRVEVVIFNAYHAYPLLRCKKDSTGQYLL 180

Query: 196 TEVVMCFDNDGVTLLNCTTTKSNCDADVLF 226
           TEVVMCF +DGVTLLNCT +KS C AD LF
Sbjct: 181 TEVVMCFKDDGVTLLNCTRSKSTCGADFLF 210

[Close]

BLAST of CsaV3_3G041400 vs. ExPASy TrEMBL
Match: A0A5D3DQ69 (Ribonuclease MC-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007950 PE=3 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 2.2e-91
Identity = 158/210 (75.24%), Postives = 178/210 (84.76%), Query Frame = 0


Query: 16  VLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIHGVWPQKG 75
           +LLFVVTLSISLFP MKSQ FD FYFVQQWPPAVC GR+G+CVG+G  YFTIHGVWPQKG
Sbjct: 1   MLLFVVTLSISLFPTMKSQIFDDFYFVQQWPPAVCAGRSGQCVGQGFRYFTIHGVWPQKG 60

Query: 76  GKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQY 135
           G+S++NCPGT+FD N+ISSL NTLHQ   DVIN  DQ  W HEWNKHG CSES++ M  Y
Sbjct: 61  GQSIVNCPGTKFDVNQISSLTNTLHQTWPDVINGKDQTFWEHEWNKHGTCSESKFQMLPY 120

Query: 136 FQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLRCKKDSSGQSLL 195
           FQMAINMKYKI+VL A+R+GG+ PNNH KAKQRVE  +F AY+AYPLLRCKKDS+GQ LL
Sbjct: 121 FQMAINMKYKIDVLGAMRVGGVVPNNHKKAKQRVEVVIFNAYHAYPLLRCKKDSTGQYLL 180

Query: 196 TEVVMCFDNDGVTLLNCTTTKSNCDADVLF 226
           TEVVMCF +DGVTLLNCT +KS C AD LF
Sbjct: 181 TEVVMCFKDDGVTLLNCTRSKSTCGADFLF 210

[Close]

BLAST of CsaV3_3G041400 vs. ExPASy TrEMBL
Match: A0A0A0LC37 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 8.9e-69
Identity = 122/210 (58.10%), Postives = 159/210 (75.71%), Query Frame = 0


Query: 16  VLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIHGVWPQKG 75
           V+ FV  L+I LFPI+KSQ FD F+FVQQWPPAVC  ++G+CVG+G   FTIHG+WPQKG
Sbjct: 9   VVFFVFVLTI-LFPIVKSQTFDDFWFVQQWPPAVCTLQSGRCVGRGTRSFTIHGLWPQKG 68

Query: 76  GKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQY 135
           G+SV NC G QFDF KI+ L N L+ +  +V+  +++F W HEWNKHG+CSES++   +Y
Sbjct: 69  GRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKY 128

Query: 136 FQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLRCKKDSSGQSLL 195
           FQ AINM++ I++LS LR GG+ PN   KAKQRVE A+ + +   P+LRCKK S+GQ LL
Sbjct: 129 FQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLL 188

Query: 196 TEVVMCFDNDGVTLLNCTTTKSNCDADVLF 226
           TE+VMCFD+DGVTL+NC   +SNC    +F
Sbjct: 189 TEIVMCFDDDGVTLINCNKARSNCAGSFIF 217

[Close]

BLAST of CsaV3_3G041400 vs. ExPASy TrEMBL
Match: A0A6J1DQN7 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111022224 PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 3.4e-68
Identity = 122/211 (57.82%), Postives = 161/211 (76.30%), Query Frame = 0


Query: 15  IVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIHGVWPQK 74
           IV +FV+ ++I L PI+KSQ FD F+FVQQWPPAVC  + G+CVG+G+  FTIHGVWPQK
Sbjct: 8   IVFMFVLAITIFL-PIVKSQTFDDFWFVQQWPPAVCSFQQGRCVGQGLRTFTIHGVWPQK 67

Query: 75  GGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQ 134
           GG SV+NCPGT FDF KISSL   L+++  +VI  D++F WSHEWNKHGVCSES+++MK 
Sbjct: 68  GGTSVVNCPGTPFDFTKISSLETDLNRVWPNVITGDNKFFWSHEWNKHGVCSESKFNMKA 127

Query: 135 YFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLRCKKDSSGQSL 194
           YFQMAINM+  I++++AL++GG+ PN   K+KQRV+ A+ T +   P+LRC+   S   L
Sbjct: 128 YFQMAINMRNSIDLMNALKVGGLAPNGLSKSKQRVQSALRTHFGKEPVLRCRGTGSNIRL 187

Query: 195 LTEVVMCFDNDGVTLLNCTTTKSNCDADVLF 226
           + E+VMCFD+DGVTL+NC    SNC    +F
Sbjct: 188 I-EIVMCFDDDGVTLINCNPVSSNCANSFIF 216

[Close]

BLAST of CsaV3_3G041400 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 133.7 bits (335), Expect = 1.9e-31
Identity = 75/221 (33.94%), Postives = 113/221 (51.13%), Query Frame = 0


Query: 14  NIVLLFVVTLSISLFPI-MKSQQFDKFYFVQQWPPAVCDGRTGKCV---GKGMYYFTIHG 73
           ++ L+ ++ +  S+F     S+ FD FYFVQQWP + CD +   C    GK    F IHG
Sbjct: 7   SLCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHG 66

Query: 74  VWPQ-KGGKSVINCPGTQ-FDFNKISSLANTLHQIMKDVI--NADDQFLWSHEWNKHGVC 133
           +WP  K G    NC  ++ FD + IS L  ++ +    +   +   +  W HEW KHG C
Sbjct: 67  LWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTC 126

Query: 134 SESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLRC 193
           SES     +YFQ A+N+K K N+L AL   GI P+    + + +  ++  +    P + C
Sbjct: 127 SESVIDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVEC 186

Query: 194 KKDSSGQSLLTEVVMCFDNDGVTLLNCTT-TKSNCDADVLF 226
            +D SG S L +V +C D  G  L+ C       C A++ F
Sbjct: 187 NRDGSGNSQLYQVYLCVDRSGSGLIECPVFPHGKCGAEIEF 227

[Close]

BLAST of CsaV3_3G041400 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 130.6 bits (327), Expect = 1.6e-30
Identity = 75/216 (34.72%), Postives = 106/216 (49.07%), Query Frame = 0


Query: 18  LFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKC---VGKGMYYFTIHGVWPQ- 77
           +F++ L   L+    +Q FD FYFV QWP A CD R   C    GK    F IHG+WP  
Sbjct: 5   IFILALQ-QLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNY 64

Query: 78  KGGKSVINC-PGTQFDFNKISSLANTLHQIMKDVI--NADDQFLWSHEWNKHGVCSESRY 137
           K G    NC P ++FD  ++S L + L +    +   + D    W+HEW KHG C+ES  
Sbjct: 65  KTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESEL 124

Query: 138 SMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLRCKKDSS 197
               YF+  + +K K N+L AL   GI P++     + +E  +       P + C KDSS
Sbjct: 125 DQHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSS 184

Query: 198 GQSLLTEVVMCFDNDGVTLLNC-TTTKSNCDADVLF 226
             S L ++ +C D      +NC       CD+ V F
Sbjct: 185 HNSQLYQIYLCVDTSASKFINCPVMPHGRCDSRVQF 219

[Close]

BLAST of CsaV3_3G041400 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 129.4 bits (324), Expect = 3.6e-30
Identity = 73/226 (32.30%), Postives = 110/226 (48.67%), Query Frame = 0


Query: 8   GRRSDINIVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCV---GKGMYY 67
           G +  +N++L  +V     LF     + FD FYFV QWP A CD     C    GK    
Sbjct: 2   GAKGCVNVLLKLLVFQ--GLFVSRPQEDFDFFYFVLQWPGAYCDTSRACCYPTSGKPAAD 61

Query: 68  FTIHGVWPQ-KGGKSVINC-PGTQFDFNKISSLANTLHQIMKDVINADDQ--FLWSHEWN 127
           F IHG+WP   GG    NC P +QFD ++IS L ++L +    +    ++    W HEW 
Sbjct: 62  FGIHGLWPNYNGGSWPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWE 121

Query: 128 KHGVCSESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAY 187
           KHG CSES     +YF+ A+ +K K N+L  L+  GI P++      ++  A+       
Sbjct: 122 KHGTCSESVMDQHEYFENALKLKQKANLLQILKNSGINPDDGFYNLDKITNAIKDGIGFT 181

Query: 188 PLLRCKKDSSGQSLLTEVVMCFDNDGVTLLNC-TTTKSNCDADVLF 226
           P + C KD    + L ++ +C D  G   + C    + +C + + F
Sbjct: 182 PGIECNKDPERNAQLHQIYICVDTSGTEFIECPVLPRGSCPSQIQF 225

[Close]

BLAST of CsaV3_3G041400 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 114.8 bits (286), Expect = 9.2e-26
Identity = 62/207 (29.95%), Postives = 100/207 (48.31%), Query Frame = 0


Query: 15  IVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKC---VGKGMYYFTIHGVW 74
           I++  ++  S+ +        F+ FY+V  WP A+CD + G C    G     F IHG+W
Sbjct: 5   IIVSLLILQSLVVSSSQTEPDFNFFYWVNYWPGAICDSQKGCCPPTKGNTASDFIIHGLW 64

Query: 75  PQ-KGGKSVINCPGTQ-FDFNKISSLANTLHQIMKD----VINADDQFLWSHEWNKHGVC 134
           PQ   G     C  T  FD +KIS L   + +   +       +++  LW HEWNKHG C
Sbjct: 65  PQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNKHGTC 124

Query: 135 SESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLRC 194
            +S +    YF+  +  K+K+++L+ L   GI PN+   +   ++ A+  A    P + C
Sbjct: 125 VQSIFDQHSYFRTNLKFKHKVHLLNILIQKGIKPNDGFYSLDEIKNAIKCAIGFAPGIEC 184

Query: 195 KKDSSGQSLLTEVVMCFDNDGVTLLNC 213
            +D  G   L ++ +C DN     + C
Sbjct: 185 NEDVKGNKQLFQIYICLDNYAKEFVEC 211

The following BLAST results are available for this feature:

BLAST of CsaV3_3G041400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147497.3	5.4e-129	100.00	ribonuclease MC [Cucumis sativus] >KAE8651218.1 hypothetical protein Csa_002248 ...	[more]
KAA0053765.1	1.5e-91	75.71	ribonuclease MC-like [Cucumis melo var. makuwa] >TYK25637.1 ribonuclease MC-like...	[more]
KAA0053767.1	4.5e-91	75.24	ribonuclease MC-like [Cucumis melo var. makuwa] >TYK25635.1 ribonuclease MC-like...	[more]
XP_004147499.1	1.8e-68	58.10	ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [C...	[more]
XP_022155091.1	7.0e-68	57.82	ribonuclease MC-like [Momordica charantia]	[more]

BLAST of CsaV3_3G041400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	6.0e-46	44.04	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P42813	2.7e-30	33.94	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P42815	2.3e-29	34.72	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
P80022	3.6e-27	33.66	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
P80196	2.0e-25	34.15	Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2	[more]

BLAST of CsaV3_3G041400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHQ9	1.1e-127	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G825060 PE=3 SV=1	[more]
A0A5A7UJ53	7.5e-92	75.71	Ribonuclease MC-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352...	[more]
A0A5D3DQ69	2.2e-91	75.24	Ribonuclease MC-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352...	[more]
A0A0A0LC37	8.9e-69	58.10	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1	[more]
A0A6J1DQN7	3.4e-68	57.82	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111022224 PE=3 SV=1	[more]

BLAST of CsaV3_3G041400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02990.1	1.9e-31	33.94	ribonuclease 1	[more]
AT1G26820.1	1.6e-30	34.72	ribonuclease 3	[more]
AT1G14220.1	3.6e-30	32.30	Ribonuclease T2 family protein	[more]
AT1G14210.1	9.2e-26	29.95	Ribonuclease T2 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 36..212 e-value: 6.3E-39 score: 134.0
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 18..220
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 36..224 e-value: 1.4E-49 score: 170.7
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 36..225
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 18..220
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 114..125
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 36..225 e-value: 2.08854E-63 score: 194.088

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_3G041400.1	CsaV3_3G041400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding