CsaV3_3G037930 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_3G037930
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Descriptionmediator of RNA polymerase II transcription subunit 27
Locationchr3: 31220133 .. 31224742 (+)
RNA-Seq ExpressionCsaV3_3G037930
SyntenyCsaV3_3G037930
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAGAAAAGAAAAGAAAAGAAAAGGGAGAGGTTGGAAATTGAGAAATTAGCGGTCGTCTTGGTCACACCTCCGAAGCCTTTTGGGGAAAGAAATTGGAAGCAGTGAACATCGTCGGAGGGGTAAATGCGACATATTCAGCAGCCATCGCAGGGCCAAACTGCGACGGTCAGCCCTAGCACTCACTCTCCCCCCTCCCCCGACGACGCTCCTCCCAAGCAGGTTGCTCTCGCCATGGACAGACTCGGCCACGCCGCTCGTCTCATCGCCGACATCAGACTCGGCGCTGATCGCCTTCTCGAAGCCCTATGCGTCACTGCTCAGCCTCATCAAAGCTCTAAGCCCCTCCATTTGTTTCAGAAAGAAGACGCTTCCATGCGCCAACACCTCCTCGACCTTCGTGCAGTTGGTACTTTGCACCATTTCCATTTTTTCTTTTCTTTTAGGGTTACGACTAAGGTCGCACTAGATTTTAATTGTTTACTTCTGATTTTGTTAGGAAAGCAGCTTGAAGAGTCTGGCGTTCTCAGTGAATCTCTTCTTTCGAGAAGTAACTCTTGGGGTCTACATATGCCTTTAGTGTGTCCTGATGGCGCTGTGGTTGCTTATGCTTGGAAACGTCAATTGGCTGGCCAGGCTGGTGCATCTGCAGTTGACCGAACAAGGTTAGTTTAGACAGCAAATTAGCTTCCCTGGGACATGAATTTGAAGAACAGCTTGAAATTTGGAAGTTTACTCGACATTTCTTCATATTGCTTAGTGATAATTTGGACTCGATCGGCTACGTGCTCGGATTGATGATATTAATAAATTCCCAAATAATTCGATCAAGTATTTTGCATGTGTAGTATATAAGTGAAGCATCGAAGGTTATAGCTGCTTTGGGGTTTATTAGGAATATTTAGATTGACTAGAGAAGAAGGAATTATCGGTTTTTTGGCCCCCCAATCGTTTGTCTCCTAATTTTTATGCGCCACCCCGCTTTCTCCCCAAAACCCCTTCATTTTCCTCTCTTTGGAAAGCTGGAATTCCAAAGAGTAAAATTTTTTGCCTGACAAAGTTAACACTTCGGATCACGTTCAGACACATTCTTAGTCGCGTCTTGGTTCTTAGTGACACATCTTGTGCAGAGGAGCTCTGGCGGATGTTGATCATTTATTTTGGAGTTGCCAATTTGTGCAATTGATTTGGTGGTATTTTTGGGACTCTTTGAGACTTCTGTCGTTTGTGATAGGGGCATGCAGCGCCATGATGGAAGTGATTGTGTTGTTCCCTCCCTTCAAGAGTATAGGTTCTACAACTCTGTCCCTGAGTTCTAAGGATAAACAAAAAGTTCTTTGGCAACCTTTTCTCCCTAAATCTTGTGAGTACGTTTGGCTTGAAAAAAAAAACAGATCTTTGAGGGTTCGGTCGGTCTTGGCATGTTTTTTGGGAGGATACCCTTGTTTAATACTTGCGATGGCCTCTAAGGCCTTTTGTCATTATCAGTTAGGTGTTATTCTTTTGGATTGGAGACCTTATTATTTATTTCTTTTGAAGGTTGGGCCTGTTCTTGTGTGGGCTGTCTTTATGTATGCCCCTTGTATCTTCCTTTATGTTTCTCAATAAAGCTCTATGTTTTATGACATGAAAAACAAGCAATAACAAGAAGAAGAATCCAAGTTGTAAGATGAATAAATGCCATGTGGAAATGATGTTTCATAGTTAACTGGTTACCATTAGGTTATGGCCTGTGTCAAACAACATATTTTGTTCACCTACAAAGGATTTTAATATTGAACACATCTCTTCTAACACAGAGCGATGAAGTTATTTAATGAATTGACCTTGAATGTGTGTTTGCTTCTACTTTACCGGAGGACAGTTTCAGTTGTTTTCGAAATTTTTAAACAATAGACAAAATCATTTAATTTTTAATCTGACTTCCATAAGTTCAGATGAGTGTTTTACTGATGCTTTGCTTGTTAATAGGTTAGCTTTAAAGGCCTTCACTGACCAAAAAAGACGGTTTTTCCCTCATCTTGAAGATGAAAATGCGTCAAATAATGAGCCTGCTCTGAAGAAGCCTTGTTTTTCTCATGGAGTGACAGATGTCAAACAAAATGAGCTTATTGATTCTAGATCACTCTCGGATATTTTATCCTGTATGGAAAAGGAAGTGCCAGAGCTTAAGATTTTCACTTATGAGCGATTGGACTGGTTAAAACAAGCTTCGGCCCTTCCCTCTCAAGCAAATGAGAATTCAATAGGAGCATTGAAAGAACATAGCTATCATAGTCCTAGGATGAGCCCTGGAGCACTTGGGGCTACTCCTGCAGATAAAGCTGGTGTGATTGAGTTGATCATTCCTTCCGTCTTTAGAGCCGTAGTATCTCTGCATCCAGTTGGTTCTGTTGAACCAGATGCTCTCGCTTTCTTTTCTCCTGATGAGGTACTCTATTTCCCGCATAAGTATTCATTTTCAAAATATTTTACTCATTATTCTGTTGTACAGACAAGAAAATTCTCCAATAAATGGTGTTATTGCCTGATATGAGGGTTTAAAGATATGCAACCACTTGATTTATGAAATAGTTGGCGCACGACATTTATTAAGTTCTATTTATATCATTCACTCACACACACGCACACATATATAATTCAAAACCAAAATTTTTAATACTATTGTATTGTTGATCGTCATTGTATTTTAACAGATCTGAGACTAGACTTGGTGTTTGTTCATTACAAATGCAATTAGACTTTTTAGTTTATTTTTCTAAAATTATTATCTCTGCTTGAAGCCTCCACCTGGTTCCGATTCATTGGTCTTTTTCCCACTGATTTTTATTGTATCTTACTTGAGTACTTAAGTTGGTAATGTTGCAGTTTATTTAGAACTTTTTGTGTTTTAACAGAGAGGCAGCCATGTACATGCCAGGGGTTTTTCAAATTATCATTTGTTCAGACATGTTACGGTACCAATCACTTTGCTCATAATGTTCACTTTCATTCACATTTATGAAGGTTGCCTTCTCTCAGAGATGATAATTTCGTTTTATCTTGATGCAGGAGCATGCTGCCACAGTGCTGCAGTATTTTCTTGGAAATCAACCAAAAGCAGCTCTTTATCCTCTATTGGTGAAACATCACTCATTGAATTTGTACCTTGAGATATTCTTTCTCCTTTTCATTTAAAACCCTCGTCAATTAATTTTTGGTACTTTCTTGTAGCTTTGGATCTGCAGCTACCAAAATTTATTTTCAAAAGCTTGTAGGTGGGTTTAAGAGTTTTTAGTGCTTCCATATTTGGTTTTGTATGGATTTGACACTTACCATCATGTGTTATCTGATCTTGCAGTAAATGTGGACGGCGTTTATCAGTAAACAAACAATCTGATTTATTGTTACCTCCTGCAGTTCGGCCGTACAAACAGTTTTGTGCATCAAAAAACTCATCAGTTGTGCCCATAACCTCATTGAAAGACCAGAAGCTGGATTCTGTTCAAGCTTTCCACATTGATTGTTTCTCAGAAGAAATATAGTCACACTTAGCGGTTCAGGTACAGTTAGAATAAACCACACTATTGTCAATTGCTTGACCATTTTTAAGCATGTAACTGATCGTGGATTTCTTTTATCTGGTAATAGTGGCAGTATGCACCAGTTCCTACTAATTAACGATTTCCTAACATTAAAATCTACTAAGCTCACTCTTTCATGGCCCATAATACAACTTTTAGTCTATGTTCGGCTGTTACTGAATAGATATGGAACATTGCAAGGGGGCTCATCCCAGACTCTTCTGCTCTGTTGGAATGCTTTAAATTTATCATCTCACGCTCCTAATAACCAAGCATGGAGTGTCATATATTACTTTTTTTTTTTTTTTTTATTGATTCTGCTTCCGTATTAGCATGTTCCTTGTCTTTCACCTGCACTGAACCTTGTTTTTCTCAACTTCACTCGTCTAATTGTTGGTTATAATTCGCTTCAGGGCCTCATGGACTCTGATTCAAATGATATACGGCAATATCCTCAGAATCAGAGGATGGCCCAGGCTGCTATGCTATCTTGTGCTGCTTTTGGTGATTGCAATTACATGCACGGAATGATATTCACATTCAGATTACACATAGGAGCTAATCTAATCGTGATTTCGAAAATGACCATCTGTTACTGATGCGGTTATTTTTGTTGTCTCAAGTCGCTTGCCATCATCAAGCCAGGCTAAAGGTACACTCATTTGCTTTTGATATGAGCAATTGCTATTTTTTGTCAATCTGCTCAGAAAGGTAACTTTGGATTTTGGAATTATTGGTATCTTTCTTTCCACTTCAGATTCAAGTGAAGGAAGTTAGGGTTTCATTAGTATGAGCGTGCATATGTACAATACTCTAAAGTTTGGATCAGCAGAAGAGCGAAAAACTTTAGTAAACATTTGAACCTCAAGAGTCAATAGTTATGAAAACATTTTGTTGGTGTTATTAATTACTCTTGAAAGTTGAATATACTATGTAATATCTTATGAAAAACTAATTGGTTATGGTTTTGACATATATTTGAGTACAACAAGAACTAAACCAAAAACATCCTATAAAACTGGAATATATATTATATATACATCTGTGTG

mRNA sequence

ATGCGACATATTCAGCAGCCATCGCAGGGCCAAACTGCGACGGTCAGCCCTAGCACTCACTCTCCCCCCTCCCCCGACGACGCTCCTCCCAAGCAGGTTGCTCTCGCCATGGACAGACTCGGCCACGCCGCTCGTCTCATCGCCGACATCAGACTCGGCGCTGATCGCCTTCTCGAAGCCCTATGCGTCACTGCTCAGCCTCATCAAAGCTCTAAGCCCCTCCATTTGTTTCAGAAAGAAGACGCTTCCATGCGCCAACACCTCCTCGACCTTCGTGCAGTTGGAAAGCAGCTTGAAGAGTCTGGCGTTCTCAGTGAATCTCTTCTTTCGAGAAGTAACTCTTGGGGTCTACATATGCCTTTAGTGTGTCCTGATGGCGCTGTGGTTGCTTATGCTTGGAAACGTCAATTGGCTGGCCAGGCTGGTGCATCTGCAGTTGACCGAACAAGGTTAGCTTTAAAGGCCTTCACTGACCAAAAAAGACGGTTTTTCCCTCATCTTGAAGATGAAAATGCGTCAAATAATGAGCCTGCTCTGAAGAAGCCTTGTTTTTCTCATGGAGTGACAGATGTCAAACAAAATGAGCTTATTGATTCTAGATCACTCTCGGATATTTTATCCTGTATGGAAAAGGAAGTGCCAGAGCTTAAGATTTTCACTTATGAGCGATTGGACTGGTTAAAACAAGCTTCGGCCCTTCCCTCTCAAGCAAATGAGAATTCAATAGGAGCATTGAAAGAACATAGCTATCATAGTCCTAGGATGAGCCCTGGAGCACTTGGGGCTACTCCTGCAGATAAAGCTGGTGTGATTGAGTTGATCATTCCTTCCGTCTTTAGAGCCGTAGTATCTCTGCATCCAGTTGGTTCTGTTGAACCAGATGCTCTCGCTTTCTTTTCTCCTGATGAGAGAGGCAGCCATGTACATGCCAGGGGTTTTTCAAATTATCATTTGTTCAGACATGTTACGGAGCATGCTGCCACAGTGCTGCAGTATTTTCTTGGAAATCAACCAAAAGCAGCTCTTTATCCTCTATTGCTTTGGATCTGCAGCTACCAAAATTTATTTTCAAAAGCTTGTAGTTCGGCCGTACAAACAGTTTTGTGCATCAAAAAACTCATCAGTTGTGCCCATAACCTCATTGAAAGACCAGAAGCTGGATTCTGTTCAAGCTTTCCACATTGA

Coding sequence (CDS)

ATGCGACATATTCAGCAGCCATCGCAGGGCCAAACTGCGACGGTCAGCCCTAGCACTCACTCTCCCCCCTCCCCCGACGACGCTCCTCCCAAGCAGGTTGCTCTCGCCATGGACAGACTCGGCCACGCCGCTCGTCTCATCGCCGACATCAGACTCGGCGCTGATCGCCTTCTCGAAGCCCTATGCGTCACTGCTCAGCCTCATCAAAGCTCTAAGCCCCTCCATTTGTTTCAGAAAGAAGACGCTTCCATGCGCCAACACCTCCTCGACCTTCGTGCAGTTGGAAAGCAGCTTGAAGAGTCTGGCGTTCTCAGTGAATCTCTTCTTTCGAGAAGTAACTCTTGGGGTCTACATATGCCTTTAGTGTGTCCTGATGGCGCTGTGGTTGCTTATGCTTGGAAACGTCAATTGGCTGGCCAGGCTGGTGCATCTGCAGTTGACCGAACAAGGTTAGCTTTAAAGGCCTTCACTGACCAAAAAAGACGGTTTTTCCCTCATCTTGAAGATGAAAATGCGTCAAATAATGAGCCTGCTCTGAAGAAGCCTTGTTTTTCTCATGGAGTGACAGATGTCAAACAAAATGAGCTTATTGATTCTAGATCACTCTCGGATATTTTATCCTGTATGGAAAAGGAAGTGCCAGAGCTTAAGATTTTCACTTATGAGCGATTGGACTGGTTAAAACAAGCTTCGGCCCTTCCCTCTCAAGCAAATGAGAATTCAATAGGAGCATTGAAAGAACATAGCTATCATAGTCCTAGGATGAGCCCTGGAGCACTTGGGGCTACTCCTGCAGATAAAGCTGGTGTGATTGAGTTGATCATTCCTTCCGTCTTTAGAGCCGTAGTATCTCTGCATCCAGTTGGTTCTGTTGAACCAGATGCTCTCGCTTTCTTTTCTCCTGATGAGAGAGGCAGCCATGTACATGCCAGGGGTTTTTCAAATTATCATTTGTTCAGACATGTTACGGAGCATGCTGCCACAGTGCTGCAGTATTTTCTTGGAAATCAACCAAAAGCAGCTCTTTATCCTCTATTGCTTTGGATCTGCAGCTACCAAAATTTATTTTCAAAAGCTTGTAGTTCGGCCGTACAAACAGTTTTGTGCATCAAAAAACTCATCAGTTGTGCCCATAACCTCATTGAAAGACCAGAAGCTGGATTCTGTTCAAGCTTTCCACATTGA

Protein sequence

MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSSAVQTVLCIKKLISCAHNLIERPEAGFCSSFPH*
Homology
BLAST of CsaV3_3G037930 vs. NCBI nr
Match: XP_031739120.1 (mediator of RNA polymerase II transcription subunit 27 isoform X2 [Cucumis sativus] >KAE8651114.1 hypothetical protein Csa_001284 [Cucumis sativus])

HSP 1 Score: 787.3 bits (2032), Expect = 6.0e-224
Identity = 394/394 (100.00%), Postives = 394/394 (100.00%), Query Frame = 0

Query: 1   MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60
           MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA
Sbjct: 1   MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60

Query: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP 120
           LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP
Sbjct: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP 120

Query: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK 180
           LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK
Sbjct: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK 180

Query: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN 240
           KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN
Sbjct: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN 240

Query: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS 300
           SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS
Sbjct: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS 300

Query: 301 PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC 360
           PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC
Sbjct: 301 PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC 360

Query: 361 SSAVQTVLCIKKLISCAHNLIERPEAGFCSSFPH 395
           SSAVQTVLCIKKLISCAHNLIERPEAGFCSSFPH
Sbjct: 361 SSAVQTVLCIKKLISCAHNLIERPEAGFCSSFPH 394

BLAST of CsaV3_3G037930 vs. NCBI nr
Match: XP_004136512.1 (mediator of RNA polymerase II transcription subunit 27 isoform X1 [Cucumis sativus])

HSP 1 Score: 721.8 bits (1862), Expect = 3.1e-204
Identity = 368/391 (94.12%), Postives = 371/391 (94.88%), Query Frame = 0

Query: 1   MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60
           MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA
Sbjct: 1   MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60

Query: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP 120
           LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP
Sbjct: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP 120

Query: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK 180
           LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK
Sbjct: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK 180

Query: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN 240
           KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN
Sbjct: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN 240

Query: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS 300
           SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS
Sbjct: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS 300

Query: 301 PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC 360
           PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC
Sbjct: 301 PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC 360

Query: 361 SSAVQTVLCIKKLISCAHNLIERPEAGFCSS 392
           S   +  L + K          RP   FC+S
Sbjct: 361 SKCGRR-LSVNKQSDLLLPPAVRPYKQFCAS 390

BLAST of CsaV3_3G037930 vs. NCBI nr
Match: XP_008442914.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 27 [Cucumis melo])

HSP 1 Score: 713.0 bits (1839), Expect = 1.4e-201
Identity = 362/391 (92.58%), Postives = 370/391 (94.63%), Query Frame = 0

Query: 1   MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60
           MRHIQQPSQGQTATVSP+THSP S DDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA
Sbjct: 1   MRHIQQPSQGQTATVSPNTHSPSSADDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60

Query: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP 120
           LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP
Sbjct: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP 120

Query: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK 180
           LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK
Sbjct: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK 180

Query: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN 240
           KPCFSHGVTDVKQNELIDSRSLSDILSC+EKEVPELKIFTYERLDWLKQASALPSQANEN
Sbjct: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCVEKEVPELKIFTYERLDWLKQASALPSQANEN 240

Query: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS 300
           SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHP+GSVEPDALAFFS
Sbjct: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPIGSVEPDALAFFS 300

Query: 301 PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC 360
           PDERGSHVHARGFSNYH+FRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC
Sbjct: 301 PDERGSHVHARGFSNYHVFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC 360

Query: 361 SSAVQTVLCIKKLISCAHNLIERPEAGFCSS 392
           S   +  L + K        + RP   FC+S
Sbjct: 361 SKCGRR-LSVNKQSDLLLPPVVRPYKQFCAS 390

BLAST of CsaV3_3G037930 vs. NCBI nr
Match: XP_038904949.1 (mediator of RNA polymerase II transcription subunit 27 [Benincasa hispida] >XP_038904950.1 mediator of RNA polymerase II transcription subunit 27 [Benincasa hispida] >XP_038904951.1 mediator of RNA polymerase II transcription subunit 27 [Benincasa hispida] >XP_038904952.1 mediator of RNA polymerase II transcription subunit 27 [Benincasa hispida])

HSP 1 Score: 686.4 bits (1770), Expect = 1.4e-193
Identity = 351/394 (89.09%), Postives = 363/394 (92.13%), Query Frame = 0

Query: 1   MRHIQQPSQGQTATVSPST---HSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MR  QQP+QGQTATVSP+T   HSP S DDAPPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQQPAQGQTATVSPNTAPPHSPASADDAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQS+KPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDEN SNNEP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENGSNNEP 180

Query: 181 ALKKPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQA 240
           ALKKPCFSHGVTDVKQNEL DSRSLS+IL+CMEKEVPELKIFTYERLDWLKQASALPSQA
Sbjct: 181 ALKKPCFSHGVTDVKQNELNDSRSLSEILTCMEKEVPELKIFTYERLDWLKQASALPSQA 240

Query: 241 NENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA 300
           NENSIG LKEHSYHSPRMSPG+LGATP DKAGVIEL+IPSVFRAVVSLHPVGSVEPDA+A
Sbjct: 241 NENSIGVLKEHSYHSPRMSPGSLGATPVDKAGVIELVIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFS 360
           FFSPDERGSHVHARGFSN+HLFRHVTEHAATVLQYFLGNQ KAALYPLLLWICSYQNLFS
Sbjct: 301 FFSPDERGSHVHARGFSNHHLFRHVTEHAATVLQYFLGNQAKAALYPLLLWICSYQNLFS 360

Query: 361 KACSSAVQTVLCIKKLISCAHNLIERPEAGFCSS 392
           KACS   Q +L + K          RP   FC+S
Sbjct: 361 KACSKCGQ-LLSVNKQSDLLLPPAVRPYKQFCAS 393

BLAST of CsaV3_3G037930 vs. NCBI nr
Match: XP_022940393.1 (mediator of RNA polymerase II transcription subunit 27 [Cucurbita moschata])

HSP 1 Score: 643.7 bits (1659), Expect = 1.1e-180
Identity = 333/394 (84.52%), Postives = 352/394 (89.34%), Query Frame = 0

Query: 1   MRHIQQPSQGQTATVSPST---HSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MR  Q P+QG TATVSP+T   HS  S ++APPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQS+KPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL++EN SN EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180

Query: 181 ALKKPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQA 240
           A KKPCFSHGVTDVKQ EL DS SLSDIL+CMEKEVP+LKIFTYERLDWLKQASALPSQA
Sbjct: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240

Query: 241 NENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA 300
           NENSIGALKEH+YHSPRMSP +L AT  DKA VIELIIPSVFRAVVSLHPVGSVEPDA+A
Sbjct: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFS 360
           FFSPDERGSHVHARGFSN+HLFRHVTEHAATVLQYF+G Q KAAL+PLLLWICSYQNLFS
Sbjct: 301 FFSPDERGSHVHARGFSNHHLFRHVTEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFS 360

Query: 361 KACSSAVQTVLCIKKLISCAHNLIERPEAGFCSS 392
           KACS   + +L + K          RP   FC+S
Sbjct: 361 KACSKCGR-LLSVNKQSDLLLPPAIRPYKQFCTS 393

BLAST of CsaV3_3G037930 vs. ExPASy Swiss-Prot
Match: Q8RWM3 (Mediator of RNA polymerase II transcription subunit 27 OS=Arabidopsis thaliana OX=3702 GN=MED27 PE=1 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 1.4e-114
Identity = 217/349 (62.18%), Postives = 265/349 (75.93%), Query Frame = 0

Query: 24  SPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDAS 83
           S  DAPPKQVA AM+RL  AAR+IADIRLGADR+LEA+ V +QP  +  PL LF +EDAS
Sbjct: 21  SESDAPPKQVAQAMERLNQAARVIADIRLGADRILEAMFVASQPRHTDMPLQLFLREDAS 80

Query: 84  MRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGA 143
           MRQHL DLR +GK+LEESGVL+ESL SRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGA
Sbjct: 81  MRQHLQDLRLIGKKLEESGVLTESLRSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGA 140

Query: 144 SAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSRSLS 203
           SAVDRTRLALKAFTDQKRRFFPH++D      EP+ KK   SH + +  + E +D ++L 
Sbjct: 141 SAVDRTRLALKAFTDQKRRFFPHIDD--GLKMEPSSKKHRASHLLLENGREEPVDYKTLP 200

Query: 204 DILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGAT 263
           DI S +EK VP +K+ TY RL+WLK+A++LP   +++   A K     S ++  G L   
Sbjct: 201 DIQSRLEKLVPSVKVSTYGRLNWLKRANSLPGSGSDDPTEASKPIFQSSSKLRSG-LQTE 260

Query: 264 PADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFSPDERGSHVHARGFSNYHLFRHVT 323
             DK  VIEL  PS+FRA+VSL P GSV+PDA+AFFSPDE GS++HARGFS YH+++H+T
Sbjct: 261 VVDKIAVIELSFPSLFRAIVSLSPAGSVDPDAVAFFSPDEGGSYLHARGFSVYHVYKHIT 320

Query: 324 EHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSSAVQTVLCIKK 373
           EHAAT LQYFLG     ALY LLLWICS++++FSK C+   + +   KK
Sbjct: 321 EHAATALQYFLGFGTGTALYSLLLWICSFESVFSKPCTKCGRLLAMDKK 366

BLAST of CsaV3_3G037930 vs. ExPASy TrEMBL
Match: A0A0A0LGT1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G781560 PE=3 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 1.5e-204
Identity = 368/391 (94.12%), Postives = 371/391 (94.88%), Query Frame = 0

Query: 1   MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60
           MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA
Sbjct: 1   MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60

Query: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP 120
           LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP
Sbjct: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP 120

Query: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK 180
           LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK
Sbjct: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK 180

Query: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN 240
           KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN
Sbjct: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN 240

Query: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS 300
           SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS
Sbjct: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS 300

Query: 301 PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC 360
           PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC
Sbjct: 301 PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC 360

Query: 361 SSAVQTVLCIKKLISCAHNLIERPEAGFCSS 392
           S   +  L + K          RP   FC+S
Sbjct: 361 SKCGRR-LSVNKQSDLLLPPAVRPYKQFCAS 390

BLAST of CsaV3_3G037930 vs. ExPASy TrEMBL
Match: A0A1S3B7L6 (mediator of RNA polymerase II transcription subunit 27 OS=Cucumis melo OX=3656 GN=LOC103486676 PE=3 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 6.9e-202
Identity = 362/391 (92.58%), Postives = 370/391 (94.63%), Query Frame = 0

Query: 1   MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60
           MRHIQQPSQGQTATVSP+THSP S DDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA
Sbjct: 1   MRHIQQPSQGQTATVSPNTHSPSSADDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60

Query: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP 120
           LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP
Sbjct: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP 120

Query: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK 180
           LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK
Sbjct: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK 180

Query: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN 240
           KPCFSHGVTDVKQNELIDSRSLSDILSC+EKEVPELKIFTYERLDWLKQASALPSQANEN
Sbjct: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCVEKEVPELKIFTYERLDWLKQASALPSQANEN 240

Query: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS 300
           SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHP+GSVEPDALAFFS
Sbjct: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPIGSVEPDALAFFS 300

Query: 301 PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC 360
           PDERGSHVHARGFSNYH+FRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC
Sbjct: 301 PDERGSHVHARGFSNYHVFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC 360

Query: 361 SSAVQTVLCIKKLISCAHNLIERPEAGFCSS 392
           S   +  L + K        + RP   FC+S
Sbjct: 361 SKCGRR-LSVNKQSDLLLPPVVRPYKQFCAS 390

BLAST of CsaV3_3G037930 vs. ExPASy TrEMBL
Match: A0A6J1FP59 (mediator of RNA polymerase II transcription subunit 27 OS=Cucurbita moschata OX=3662 GN=LOC111446014 PE=3 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 5.2e-181
Identity = 333/394 (84.52%), Postives = 352/394 (89.34%), Query Frame = 0

Query: 1   MRHIQQPSQGQTATVSPST---HSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MR  Q P+QG TATVSP+T   HS  S ++APPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQS+KPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL++EN SN EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180

Query: 181 ALKKPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQA 240
           A KKPCFSHGVTDVKQ EL DS SLSDIL+CMEKEVP+LKIFTYERLDWLKQASALPSQA
Sbjct: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240

Query: 241 NENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA 300
           NENSIGALKEH+YHSPRMSP +L AT  DKA VIELIIPSVFRAVVSLHPVGSVEPDA+A
Sbjct: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFS 360
           FFSPDERGSHVHARGFSN+HLFRHVTEHAATVLQYF+G Q KAAL+PLLLWICSYQNLFS
Sbjct: 301 FFSPDERGSHVHARGFSNHHLFRHVTEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFS 360

Query: 361 KACSSAVQTVLCIKKLISCAHNLIERPEAGFCSS 392
           KACS   + +L + K          RP   FC+S
Sbjct: 361 KACSKCGR-LLSVNKQSDLLLPPAIRPYKQFCTS 393

BLAST of CsaV3_3G037930 vs. ExPASy TrEMBL
Match: A0A6J1KZZ9 (mediator of RNA polymerase II transcription subunit 27 OS=Cucurbita maxima OX=3661 GN=LOC111498620 PE=3 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 4.4e-180
Identity = 331/394 (84.01%), Postives = 350/394 (88.83%), Query Frame = 0

Query: 1   MRHIQQPSQGQTATVSPST---HSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MR  Q P+QG TATVSP+T   HS  S ++APPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120
           LEALCVTAQPHQS+KPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL++EN SN EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180

Query: 181 ALKKPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQA 240
           A KKPCFSHGVTDVKQ EL DS SLSDIL+CMEKEVP+LKIFTYERLDWLKQAS LPSQA
Sbjct: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASVLPSQA 240

Query: 241 NENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA 300
           NENSIG LKEH+YHSPRMSP +L AT  DKA VIELIIPSVFRAVVSLHPVGSVEPDA+A
Sbjct: 241 NENSIGTLKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFS 360
           FFSPDERGSHVHARGFSN+HLFRHVTEHAATVLQYF+G Q KAAL+PLLLWICSYQNLFS
Sbjct: 301 FFSPDERGSHVHARGFSNHHLFRHVTEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFS 360

Query: 361 KACSSAVQTVLCIKKLISCAHNLIERPEAGFCSS 392
           KACS   + +L + K          RP   FC+S
Sbjct: 361 KACSKCGR-LLSVNKQSDLLLPPAIRPYKQFCTS 393

BLAST of CsaV3_3G037930 vs. ExPASy TrEMBL
Match: A0A6J1DC62 (mediator of RNA polymerase II transcription subunit 27 OS=Momordica charantia OX=3673 GN=LOC111019372 PE=3 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 1.9e-175
Identity = 322/394 (81.73%), Postives = 348/394 (88.32%), Query Frame = 0

Query: 1   MRHIQQPSQGQTATVSPST---HSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MR  QQP+QGQTATVSP+T   HS  S ++APPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120
           LEALCVTA PHQS+KPLHLFQKED SMRQHL DLRAVGKQLEESGVLSESLLSRSNSWGL
Sbjct: 61  LEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL+DE+  N EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEP 180

Query: 181 ALKKPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQA 240
           ALKKPCFSHGVT++KQ+EL + R+LSDIL+CMEKEVP+LKIFTYERLDWLKQA AL SQA
Sbjct: 181 ALKKPCFSHGVTEIKQDELNEPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQA 240

Query: 241 NENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA 300
           N+NSIGALKEH+YHSPRM PG+L A+  DK  VIELIIPSVFRAVVSLHPVGSVEPDA+A
Sbjct: 241 NDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFS 360
           FF+PDERGS+VHARGFS +HLFRHVTEHAATVLQYFLG Q KAALYPLLLWICSYQNLFS
Sbjct: 301 FFAPDERGSYVHARGFSVHHLFRHVTEHAATVLQYFLGIQEKAALYPLLLWICSYQNLFS 360

Query: 361 KACSSAVQTVLCIKKLISCAHNLIERPEAGFCSS 392
           KACS   + +L + K          RP   FC+S
Sbjct: 361 KACSKCGR-LLSVNKQSDLLLPPAIRPYKQFCTS 393

BLAST of CsaV3_3G037930 vs. TAIR 10
Match: AT3G09180.1 (CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med27 (InterPro:IPR021627); Has 112 Blast hits to 112 proteins in 38 species: Archae - 0; Bacteria - 0; Metazoa - 79; Fungi - 0; Plants - 23; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink). )

HSP 1 Score: 414.5 bits (1064), Expect = 9.8e-116
Identity = 217/349 (62.18%), Postives = 265/349 (75.93%), Query Frame = 0

Query: 24  SPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDAS 83
           S  DAPPKQVA AM+RL  AAR+IADIRLGADR+LEA+ V +QP  +  PL LF +EDAS
Sbjct: 21  SESDAPPKQVAQAMERLNQAARVIADIRLGADRILEAMFVASQPRHTDMPLQLFLREDAS 80

Query: 84  MRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGA 143
           MRQHL DLR +GK+LEESGVL+ESL SRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGA
Sbjct: 81  MRQHLQDLRLIGKKLEESGVLTESLRSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGA 140

Query: 144 SAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSRSLS 203
           SAVDRTRLALKAFTDQKRRFFPH++D      EP+ KK   SH + +  + E +D ++L 
Sbjct: 141 SAVDRTRLALKAFTDQKRRFFPHIDD--GLKMEPSSKKHRASHLLLENGREEPVDYKTLP 200

Query: 204 DILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGAT 263
           DI S +EK VP +K+ TY RL+WLK+A++LP   +++   A K     S ++  G L   
Sbjct: 201 DIQSRLEKLVPSVKVSTYGRLNWLKRANSLPGSGSDDPTEASKPIFQSSSKLRSG-LQTE 260

Query: 264 PADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFSPDERGSHVHARGFSNYHLFRHVT 323
             DK  VIEL  PS+FRA+VSL P GSV+PDA+AFFSPDE GS++HARGFS YH+++H+T
Sbjct: 261 VVDKIAVIELSFPSLFRAIVSLSPAGSVDPDAVAFFSPDEGGSYLHARGFSVYHVYKHIT 320

Query: 324 EHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSSAVQTVLCIKK 373
           EHAAT LQYFLG     ALY LLLWICS++++FSK C+   + +   KK
Sbjct: 321 EHAATALQYFLGFGTGTALYSLLLWICSFESVFSKPCTKCGRLLAMDKK 366

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_031739120.16.0e-224100.00mediator of RNA polymerase II transcription subunit 27 isoform X2 [Cucumis sativ... [more]
XP_004136512.13.1e-20494.12mediator of RNA polymerase II transcription subunit 27 isoform X1 [Cucumis sativ... [more]
XP_008442914.11.4e-20192.58PREDICTED: mediator of RNA polymerase II transcription subunit 27 [Cucumis melo][more]
XP_038904949.11.4e-19389.09mediator of RNA polymerase II transcription subunit 27 [Benincasa hispida] >XP_0... [more]
XP_022940393.11.1e-18084.52mediator of RNA polymerase II transcription subunit 27 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q8RWM31.4e-11462.18Mediator of RNA polymerase II transcription subunit 27 OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A0A0LGT11.5e-20494.12Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G781560 PE=3 SV=1[more]
A0A1S3B7L66.9e-20292.58mediator of RNA polymerase II transcription subunit 27 OS=Cucumis melo OX=3656 G... [more]
A0A6J1FP595.2e-18184.52mediator of RNA polymerase II transcription subunit 27 OS=Cucurbita moschata OX=... [more]
A0A6J1KZZ94.4e-18084.01mediator of RNA polymerase II transcription subunit 27 OS=Cucurbita maxima OX=36... [more]
A0A6J1DC621.9e-17581.73mediator of RNA polymerase II transcription subunit 27 OS=Momordica charantia OX... [more]
Match NameE-valueIdentityDescription
AT3G09180.19.8e-11662.18CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med27 (InterPro:IPR021627);... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021627Mediator complex, subunit Med27PFAMPF11571Med27coord: 314..362
e-value: 4.0E-13
score: 49.6
IPR021627Mediator complex, subunit Med27PANTHERPTHR1313034 KDA TRANSCRIPTIONAL CO-ACTIVATOR-RELATEDcoord: 4..367
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR13130:SF5BNAC03G36240D PROTEINcoord: 4..367

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_3G037930.1CsaV3_3G037930.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016592 mediator complex