CsaV3_3G008770 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_3G008770
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionMechanosensitive ion channel protein 1
Locationchr3: 7320397 .. 7323142 (-)
RNA-Seq ExpressionCsaV3_3G008770
SyntenyCsaV3_3G008770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATGCAGAAGGTTTGCTGCGTGCTCATTTCAATCTTTCAAGCCTTACACAAAGATTTCCGAATACTTTGACCGAGTTCTTAGACCGAATGTTGTTCTAAATAAAAGATACTATAAAGATGAATCTCACAGTTTCAAAAGAGCCACTGTACGTGGTTTACATATCCCAAGTTATGGTACACATCGCATTGATACATTTCTGCCTGCATCGTCAATGAAATACTGGAACACTATCCCTCTTACTTGTTTTGGGAACTTGTCAAATTATCGGAATTATTCGTCAGCTTCTGGTAGAAAAGCTGATATAAGTGGAGATACTGGTGTGCCAGCAGCTTCTTCGGGTGTTGAACCGGATATCAGTAACCCCCCTGATGTTGGAAGGGACTTGCTTGAAAAGGTTAAGGATGTCTGGCAAAGTGCTGTTGATGCAGCATCTTTTACGGGGCAGAAGGCTAAAGAAGTTTCGGATGAATTATCTCCGCATGTAGATAAACTACTCGATTCACATCCGTATCTAAAAAATGTGATTGTTCCTGTGAGTATGACCTTGGGTGCTACTCTTTTGGCTTGGCTGGTAATGCCTAGGCTTTTGAAGAGATTTCATAAGTATTCAATGCGAAGTCCTGTTGCCATAATATCTGGATCCTTACCTAGTGAGGAAATTCCATATGAGAAAAGTTTTTGGGGTGCACTCGAGGATCCTTTGCGATATCTTGTTACCTTTTTTGCATTTTCTCAAATGTTAGTGGCTCTAACTTAAATATATTTCTCTTAATTATATGCCAGTCTCTTGGCTATGTACTTTAACTTTATCTTCCCTAACATTATTTGTTCAAACTTTGACTTGCAGTGGCGTGATAGTTGCACCAACTGCAGTAGCACCGGAGTTTGTTTCACAGGCATGCAGGGGGGCTGTAATCCTTTCCCTTGTCTGGTTTATTTATCGCTGGAAAACAAATGTGTTATCTCGAGCCTTGGCCACCAAGACTTTTGCAGGGCTTGATCGGGACAGGTTACTTACCCTTGACAAAGTTTCATCTGTTGCCCTATTTGCTATTGGATTGATGGCTTTGGCCGAGGCATCTGGTGTGGCTGTTCAATCTATGTTGACGGTTGGTGGTATTGGAGGTGAGAGCTTGGTGCCAAATGACTTTGCAATTATGTTTCCATTTTACTGATTCCTAGTTCACCGTCCTTTCTATTGTCTCCACTAACTGCTTTTTTTAGCCAACATTTCTCTTGATAAATAGACATTGTACGTGGTTACTTATATATTATTGATGAATAGGGATCTGCTCAAGATAACAACCATGTTCTTGGTTCTTTCATCTCCTTTTTCCTCTATGTAGGAGTGGCCACTGCTTTTGCTGCGAGGGATATACTTGGGAACATTTTGAGTGGCTTAACGATGCAGTTTTCACAACCCTTCTCACTTGGAGATACAATTAAGGTGTGTGTAATCTTTTTCTCTCTGCATTTTCTGGAAGGGTATGGGATTTAAAGAACAATGGAAACTCTAGACTGTCTAGGCAAGTTTTTTCTTGTTAACTTGCAATCAAATGAATTGAAAATTTCGATTTGCAGGCTGGTGCTATTGAAGGTCAAGTTGTGGAAATGGGACTCATGACAACATCGCTATTAAGTGCTGAAAAATTTCCAGTCGTTGTTCCAAATTCTCTGTTTTCTAGCCAGGTGAACCATCATCTCTTTGTCCTTCAACATTTATTGTTTTTGGCCTTTATTTTTATTCCTATATTATCACTGTATGCCTGTACCTTTTTACCTGGTCCCCAATCATACCCGCCTATTTGCTTTCGTTTAGAGTTTTTCTTCTGCTAACTTCTGTATTTTTCAAATAAATTTCTAAAATAAGTTACTTATCCACTTTCAATATCCAACTTTTTGTACCAAAAATTATTTGCATTTCTAGCTCATTGAGAAGAAAATTTGAGCTATTAACTTTGTAGACTTAGAAATGGTCGTAGTCATTACTCAAGTGGTGATTATTCTAGAAAACTATCTTGCCATTTCTATCATCTATGTTTGATTTTCAAATTTTTGCAGAGATTCTTAGCAACTCTCATATGCTATGTAGTGTCACAATATTTATATTTTTGGATGAATCTCTCTAGGGATTTGCTCATTTGGCTCCATATGCTTCAACCCATTTCAGTTGTGTTGGATACTGTGACGGAGTTCTTGGATTAAGACAGTGGTTTAATTCTTGGATTTACTTTTTTACATTTTGGTTATTCTACAGTTTCCGTTAAAATGAGATGGTAACTTTTGTAGGTAATTATAAATAAGTCAAGGGCCCAGTGGCGTGCAATCGTGAAGAGGGTTCCTTTGCAAGTTGATGATATTAGTACAGTTTCTCAGATAACAGATGATATTAAGAATATGCTTACGTCTCACCCCAAGGTGTTCTTGGGCAAGGAGGCTCCTTATTGTTTTCTCTCTCAAATTGAAAGTACATATGCAGAATTGACATTAGGTTGTAACCTTAGAAAAATGGTAAGTCTAATATTCTTTGTGGTCATCCTACCTGTTATGGCTTATGCACGCTAAGCCTCGGACATGTTGTCCTTAAAAATTTTACAATTACATACTTCAGAATGGTTCTTGTGTAGGGTAAAGATGAATCGTTCTCTACAGAGCAAGATATTCTCCTTCAGTCAGTACAAATCATTAAGGCGAAAGGTGCAACCTTAGGAAGCACCATGTCAGATTGGACGAACAAGTAA

mRNA sequence

ATGGCATGCAGAAGGTTTGCTGCGTGCTCATTTCAATCTTTCAAGCCTTACACAAAGATTTCCGAATACTTTGACCGAGTTCTTAGACCGAATGTTGTTCTAAATAAAAGATACTATAAAGATGAATCTCACAGTTTCAAAAGAGCCACTGTACGTGGTTTACATATCCCAAGTTATGGTACACATCGCATTGATACATTTCTGCCTGCATCGTCAATGAAATACTGGAACACTATCCCTCTTACTTGTTTTGGGAACTTGTCAAATTATCGGAATTATTCGTCAGCTTCTGGTAGAAAAGCTGATATAAGTGGAGATACTGGTGTGCCAGCAGCTTCTTCGGGTGTTGAACCGGATATCAGTAACCCCCCTGATGTTGGAAGGGACTTGCTTGAAAAGGTTAAGGATGTCTGGCAAAGTGCTGTTGATGCAGCATCTTTTACGGGGCAGAAGGCTAAAGAAGTTTCGGATGAATTATCTCCGCATGTAGATAAACTACTCGATTCACATCCGTATCTAAAAAATGTGATTGTTCCTGTGAGTATGACCTTGGGTGCTACTCTTTTGGCTTGGCTGGTAATGCCTAGGCTTTTGAAGAGATTTCATAAGTATTCAATGCGAAGTCCTGTTGCCATAATATCTGGATCCTTACCTAGTGAGGAAATTCCATATGAGAAAAGTTTTTGGGGTGCACTCGAGGATCCTTTGCGATATCTTGTTACCTTTTTTGCATTTTCTCAAATTGGCGTGATAGTTGCACCAACTGCAGTAGCACCGGAGTTTGTTTCACAGGCATGCAGGGGGGCTGTAATCCTTTCCCTTGTCTGGTTTATTTATCGCTGGAAAACAAATGTGTTATCTCGAGCCTTGGCCACCAAGACTTTTGCAGGGCTTGATCGGGACAGGTTACTTACCCTTGACAAAGTTTCATCTGTTGCCCTATTTGCTATTGGATTGATGGCTTTGGCCGAGGCATCTGGTGTGGCTGTTCAATCTATGTTGACGGTTGGTGGTATTGGAGGAGTGGCCACTGCTTTTGCTGCGAGGGATATACTTGGGAACATTTTGAGTGGCTTAACGATGCAGTTTTCACAACCCTTCTCACTTGGAGATACAATTAAGGCTGGTGCTATTGAAGGTCAAGTTGTGGAAATGGGACTCATGACAACATCGCTATTAAGTGCTGAAAAATTTCCAGTCGTTGTTCCAAATTCTCTGTTTTCTAGCCAGGTAATTATAAATAAGTCAAGGGCCCAGTGGCGTGCAATCGTGAAGAGGGTTCCTTTGCAAGTTGATGATATTAGTACAGTTTCTCAGATAACAGATGATATTAAGAATATGCTTACGTCTCACCCCAAGGTGTTCTTGGGCAAGGAGGCTCCTTATTGTTTTCTCTCTCAAATTGAAAGTACATATGCAGAATTGACATTAGGTTGTAACCTTAGAAAAATGGGTAAAGATGAATCGTTCTCTACAGAGCAAGATATTCTCCTTCAGTCAGTACAAATCATTAAGGCGAAAGGTGCAACCTTAGGAAGCACCATGTCAGATTGGACGAACAAGTAA

Coding sequence (CDS)

ATGGCATGCAGAAGGTTTGCTGCGTGCTCATTTCAATCTTTCAAGCCTTACACAAAGATTTCCGAATACTTTGACCGAGTTCTTAGACCGAATGTTGTTCTAAATAAAAGATACTATAAAGATGAATCTCACAGTTTCAAAAGAGCCACTGTACGTGGTTTACATATCCCAAGTTATGGTACACATCGCATTGATACATTTCTGCCTGCATCGTCAATGAAATACTGGAACACTATCCCTCTTACTTGTTTTGGGAACTTGTCAAATTATCGGAATTATTCGTCAGCTTCTGGTAGAAAAGCTGATATAAGTGGAGATACTGGTGTGCCAGCAGCTTCTTCGGGTGTTGAACCGGATATCAGTAACCCCCCTGATGTTGGAAGGGACTTGCTTGAAAAGGTTAAGGATGTCTGGCAAAGTGCTGTTGATGCAGCATCTTTTACGGGGCAGAAGGCTAAAGAAGTTTCGGATGAATTATCTCCGCATGTAGATAAACTACTCGATTCACATCCGTATCTAAAAAATGTGATTGTTCCTGTGAGTATGACCTTGGGTGCTACTCTTTTGGCTTGGCTGGTAATGCCTAGGCTTTTGAAGAGATTTCATAAGTATTCAATGCGAAGTCCTGTTGCCATAATATCTGGATCCTTACCTAGTGAGGAAATTCCATATGAGAAAAGTTTTTGGGGTGCACTCGAGGATCCTTTGCGATATCTTGTTACCTTTTTTGCATTTTCTCAAATTGGCGTGATAGTTGCACCAACTGCAGTAGCACCGGAGTTTGTTTCACAGGCATGCAGGGGGGCTGTAATCCTTTCCCTTGTCTGGTTTATTTATCGCTGGAAAACAAATGTGTTATCTCGAGCCTTGGCCACCAAGACTTTTGCAGGGCTTGATCGGGACAGGTTACTTACCCTTGACAAAGTTTCATCTGTTGCCCTATTTGCTATTGGATTGATGGCTTTGGCCGAGGCATCTGGTGTGGCTGTTCAATCTATGTTGACGGTTGGTGGTATTGGAGGAGTGGCCACTGCTTTTGCTGCGAGGGATATACTTGGGAACATTTTGAGTGGCTTAACGATGCAGTTTTCACAACCCTTCTCACTTGGAGATACAATTAAGGCTGGTGCTATTGAAGGTCAAGTTGTGGAAATGGGACTCATGACAACATCGCTATTAAGTGCTGAAAAATTTCCAGTCGTTGTTCCAAATTCTCTGTTTTCTAGCCAGGTAATTATAAATAAGTCAAGGGCCCAGTGGCGTGCAATCGTGAAGAGGGTTCCTTTGCAAGTTGATGATATTAGTACAGTTTCTCAGATAACAGATGATATTAAGAATATGCTTACGTCTCACCCCAAGGTGTTCTTGGGCAAGGAGGCTCCTTATTGTTTTCTCTCTCAAATTGAAAGTACATATGCAGAATTGACATTAGGTTGTAACCTTAGAAAAATGGGTAAAGATGAATCGTTCTCTACAGAGCAAGATATTCTCCTTCAGTCAGTACAAATCATTAAGGCGAAAGGTGCAACCTTAGGAAGCACCATGTCAGATTGGACGAACAAGTAA

Protein sequence

MACRRFAACSFQSFKPYTKISEYFDRVLRPNVVLNKRYYKDESHSFKRATVRGLHIPSYGTHRIDTFLPASSMKYWNTIPLTCFGNLSNYRNYSSASGRKADISGDTGVPAASSGVEPDISNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPVSMTLGATLLAWLVMPRLLKRFHKYSMRSPVAIISGSLPSEEIPYEKSFWGALEDPLRYLVTFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVLSRALATKTFAGLDRDRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNILSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQWRAIVKRVPLQVDDISTVSQITDDIKNMLTSHPKVFLGKEAPYCFLSQIESTYAELTLGCNLRKMGKDESFSTEQDILLQSVQIIKAKGATLGSTMSDWTNK*
Homology
BLAST of CsaV3_3G008770 vs. NCBI nr
Match: XP_004133856.1 (mechanosensitive ion channel protein 1, mitochondrial isoform X1 [Cucumis sativus] >XP_011650739.1 mechanosensitive ion channel protein 1, mitochondrial isoform X1 [Cucumis sativus] >XP_031739320.1 mechanosensitive ion channel protein 1, mitochondrial isoform X1 [Cucumis sativus] >KGN56499.1 hypothetical protein Csa_010096 [Cucumis sativus])

HSP 1 Score: 1017.3 bits (2629), Expect = 4.7e-293
Identity = 521/521 (100.00%), Postives = 521/521 (100.00%), Query Frame = 0

Query: 1   MACRRFAACSFQSFKPYTKISEYFDRVLRPNVVLNKRYYKDESHSFKRATVRGLHIPSYG 60
           MACRRFAACSFQSFKPYTKISEYFDRVLRPNVVLNKRYYKDESHSFKRATVRGLHIPSYG
Sbjct: 1   MACRRFAACSFQSFKPYTKISEYFDRVLRPNVVLNKRYYKDESHSFKRATVRGLHIPSYG 60

Query: 61  THRIDTFLPASSMKYWNTIPLTCFGNLSNYRNYSSASGRKADISGDTGVPAASSGVEPDI 120
           THRIDTFLPASSMKYWNTIPLTCFGNLSNYRNYSSASGRKADISGDTGVPAASSGVEPDI
Sbjct: 61  THRIDTFLPASSMKYWNTIPLTCFGNLSNYRNYSSASGRKADISGDTGVPAASSGVEPDI 120

Query: 121 SNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPV 180
           SNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPV
Sbjct: 121 SNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPV 180

Query: 181 SMTLGATLLAWLVMPRLLKRFHKYSMRSPVAIISGSLPSEEIPYEKSFWGALEDPLRYLV 240
           SMTLGATLLAWLVMPRLLKRFHKYSMRSPVAIISGSLPSEEIPYEKSFWGALEDPLRYLV
Sbjct: 181 SMTLGATLLAWLVMPRLLKRFHKYSMRSPVAIISGSLPSEEIPYEKSFWGALEDPLRYLV 240

Query: 241 TFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVLSRALATKTFAGLDR 300
           TFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVLSRALATKTFAGLDR
Sbjct: 241 TFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVLSRALATKTFAGLDR 300

Query: 301 DRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNILSGLT 360
           DRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNILSGLT
Sbjct: 301 DRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNILSGLT 360

Query: 361 MQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQW 420
           MQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQW
Sbjct: 361 MQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQW 420

Query: 421 RAIVKRVPLQVDDISTVSQITDDIKNMLTSHPKVFLGKEAPYCFLSQIESTYAELTLGCN 480
           RAIVKRVPLQVDDISTVSQITDDIKNMLTSHPKVFLGKEAPYCFLSQIESTYAELTLGCN
Sbjct: 421 RAIVKRVPLQVDDISTVSQITDDIKNMLTSHPKVFLGKEAPYCFLSQIESTYAELTLGCN 480

Query: 481 LRKMGKDESFSTEQDILLQSVQIIKAKGATLGSTMSDWTNK 522
           LRKMGKDESFSTEQDILLQSVQIIKAKGATLGSTMSDWTNK
Sbjct: 481 LRKMGKDESFSTEQDILLQSVQIIKAKGATLGSTMSDWTNK 521

BLAST of CsaV3_3G008770 vs. NCBI nr
Match: XP_008438011.1 (PREDICTED: mechanosensitive ion channel protein 1, mitochondrial [Cucumis melo] >XP_008438013.1 PREDICTED: mechanosensitive ion channel protein 1, mitochondrial [Cucumis melo])

HSP 1 Score: 972.2 bits (2512), Expect = 1.7e-279
Identity = 496/521 (95.20%), Postives = 507/521 (97.31%), Query Frame = 0

Query: 1   MACRRFAACSFQSFKPYTKISEYFDRVLRPNVVLNKRYYKDESHSFKRATVRGLHIPSYG 60
           MACRRFAACSFQSFKPYTKIS+Y DRVLRPNVVLNKRYY DESHSFKRATV GLHIPSYG
Sbjct: 1   MACRRFAACSFQSFKPYTKISKYVDRVLRPNVVLNKRYYNDESHSFKRATVCGLHIPSYG 60

Query: 61  THRIDTFLPASSMKYWNTIPLTCFGNLSNYRNYSSASGRKADISGDTGVPAASSGVEPDI 120
           THRIDTFLPASSMKYWNTIPLT  GNLSNYRNYSS SGRKADI+GDTGVPAAS+GVEPDI
Sbjct: 61  THRIDTFLPASSMKYWNTIPLTSIGNLSNYRNYSSTSGRKADITGDTGVPAASAGVEPDI 120

Query: 121 SNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPV 180
           SNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPV
Sbjct: 121 SNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPV 180

Query: 181 SMTLGATLLAWLVMPRLLKRFHKYSMRSPVAIISGSLPSEEIPYEKSFWGALEDPLRYLV 240
           SMTL  TLLAW VMPRLL+RFHKYSMRSPVAIISG+LPSEEIPYEKSFWGALEDPLRYLV
Sbjct: 181 SMTLAGTLLAWAVMPRLLRRFHKYSMRSPVAIISGALPSEEIPYEKSFWGALEDPLRYLV 240

Query: 241 TFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVLSRALATKTFAGLDR 300
           TFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNV SRALAT+T AGLDR
Sbjct: 241 TFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVFSRALATQTSAGLDR 300

Query: 301 DRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNILSGLT 360
           DRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARD+LGNILSGLT
Sbjct: 301 DRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDVLGNILSGLT 360

Query: 361 MQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQW 420
           MQFSQPFSLGDTIKAG IEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQW
Sbjct: 361 MQFSQPFSLGDTIKAGTIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQW 420

Query: 421 RAIVKRVPLQVDDISTVSQITDDIKNMLTSHPKVFLGKEAPYCFLSQIESTYAELTLGCN 480
           RAIVKRVPLQVDDISTVSQITDDIK ML SHPKVFLGKEAPYCFLSQIESTYAELT+GCN
Sbjct: 421 RAIVKRVPLQVDDISTVSQITDDIKKMLMSHPKVFLGKEAPYCFLSQIESTYAELTVGCN 480

Query: 481 LRKMGKDESFSTEQDILLQSVQIIKAKGATLGSTMSDWTNK 522
           L+KMGKDESFSTEQDILLQSVQIIKA+GATLGST+SDWTNK
Sbjct: 481 LKKMGKDESFSTEQDILLQSVQIIKARGATLGSTVSDWTNK 521

BLAST of CsaV3_3G008770 vs. NCBI nr
Match: KAA0048955.1 (mechanosensitive ion channel protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 963.4 bits (2489), Expect = 8.1e-277
Identity = 492/517 (95.16%), Postives = 503/517 (97.29%), Query Frame = 0

Query: 4   RRFAACSFQSFKPYTKISEYFDRVLRPNVVLNKRYYKDESHSFKRATVRGLHIPSYGTHR 63
           RRFAACSFQSFKPYTKIS+Y DRVLRPNVVLNKRYY DESHSFKRATV GLHIPSYGTHR
Sbjct: 35  RRFAACSFQSFKPYTKISKYVDRVLRPNVVLNKRYYNDESHSFKRATVCGLHIPSYGTHR 94

Query: 64  IDTFLPASSMKYWNTIPLTCFGNLSNYRNYSSASGRKADISGDTGVPAASSGVEPDISNP 123
           IDTFLPASSMKYWNTIPLT  GNLSNYRNYSS SGRKADI+GDTGVPAAS+GVEPDISNP
Sbjct: 95  IDTFLPASSMKYWNTIPLTSIGNLSNYRNYSSTSGRKADITGDTGVPAASAGVEPDISNP 154

Query: 124 PDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPVSMT 183
           PDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPVSMT
Sbjct: 155 PDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPVSMT 214

Query: 184 LGATLLAWLVMPRLLKRFHKYSMRSPVAIISGSLPSEEIPYEKSFWGALEDPLRYLVTFF 243
           L  TLLAW VMPRLL+RFHKYSMRSPVAIISG+LPSEEIPYEKSFWGALEDPLRYLVTFF
Sbjct: 215 LAGTLLAWAVMPRLLRRFHKYSMRSPVAIISGALPSEEIPYEKSFWGALEDPLRYLVTFF 274

Query: 244 AFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVLSRALATKTFAGLDRDRL 303
           AFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNV SRALAT+T AGLDRDRL
Sbjct: 275 AFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVFSRALATQTSAGLDRDRL 334

Query: 304 LTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNILSGLTMQF 363
           LTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARD+LGNILSGLTMQF
Sbjct: 335 LTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDVLGNILSGLTMQF 394

Query: 364 SQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQWRAI 423
           SQPFSLGDTIKAG IEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQWRAI
Sbjct: 395 SQPFSLGDTIKAGTIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQWRAI 454

Query: 424 VKRVPLQVDDISTVSQITDDIKNMLTSHPKVFLGKEAPYCFLSQIESTYAELTLGCNLRK 483
           VKRVPLQVDDISTVSQITDDIK ML SHPKVFLGKEAPYCFLSQIESTYAELT+GCNL+K
Sbjct: 455 VKRVPLQVDDISTVSQITDDIKKMLMSHPKVFLGKEAPYCFLSQIESTYAELTVGCNLKK 514

Query: 484 MGKDESFSTEQDILLQSVQIIKAKGATLGSTMSDWTN 521
           MGKDESFSTEQDILLQSVQIIKA+GATLGST+SDWTN
Sbjct: 515 MGKDESFSTEQDILLQSVQIIKARGATLGSTVSDWTN 551

BLAST of CsaV3_3G008770 vs. NCBI nr
Match: TYK17612.1 (mechanosensitive ion channel protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 960.3 bits (2481), Expect = 6.8e-276
Identity = 490/516 (94.96%), Postives = 502/516 (97.29%), Query Frame = 0

Query: 5   RFAACSFQSFKPYTKISEYFDRVLRPNVVLNKRYYKDESHSFKRATVRGLHIPSYGTHRI 64
           RFAACSFQSFKPYT IS+Y DRVLRPNVVLNKRYYKDESHSFKRATV GLHIP+YGTHRI
Sbjct: 54  RFAACSFQSFKPYTNISKYVDRVLRPNVVLNKRYYKDESHSFKRATVCGLHIPNYGTHRI 113

Query: 65  DTFLPASSMKYWNTIPLTCFGNLSNYRNYSSASGRKADISGDTGVPAASSGVEPDISNPP 124
           DTFLPASSMKYWNTIPLT  GNLSNYRNYSS SGRKADI+GDTGVPAAS+GVEPDISNPP
Sbjct: 114 DTFLPASSMKYWNTIPLTSIGNLSNYRNYSSTSGRKADITGDTGVPAASAGVEPDISNPP 173

Query: 125 DVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPVSMTL 184
           DVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPVSMTL
Sbjct: 174 DVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPVSMTL 233

Query: 185 GATLLAWLVMPRLLKRFHKYSMRSPVAIISGSLPSEEIPYEKSFWGALEDPLRYLVTFFA 244
             TLLAW VMPRLL+RFHKYSMRSPVAIISG+LPSEEIPYEKSFWGALEDPLRYLVTFFA
Sbjct: 234 AGTLLAWAVMPRLLRRFHKYSMRSPVAIISGALPSEEIPYEKSFWGALEDPLRYLVTFFA 293

Query: 245 FSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVLSRALATKTFAGLDRDRLL 304
           FSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNV SRALAT+T AGLDRDRLL
Sbjct: 294 FSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVFSRALATQTSAGLDRDRLL 353

Query: 305 TLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNILSGLTMQFS 364
           TLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARD+LGNILSGLTMQFS
Sbjct: 354 TLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDVLGNILSGLTMQFS 413

Query: 365 QPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQWRAIV 424
           QPFSLGDTIKAG IEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQWRAIV
Sbjct: 414 QPFSLGDTIKAGTIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQWRAIV 473

Query: 425 KRVPLQVDDISTVSQITDDIKNMLTSHPKVFLGKEAPYCFLSQIESTYAELTLGCNLRKM 484
           KRVPLQVDDISTVSQITDDIK ML SHPKVFLGKEAPYCFLSQIESTYAELT+GCNL+KM
Sbjct: 474 KRVPLQVDDISTVSQITDDIKKMLMSHPKVFLGKEAPYCFLSQIESTYAELTVGCNLKKM 533

Query: 485 GKDESFSTEQDILLQSVQIIKAKGATLGSTMSDWTN 521
           GKDESFSTEQDILLQSVQIIKA+GATLGST+SDWTN
Sbjct: 534 GKDESFSTEQDILLQSVQIIKARGATLGSTVSDWTN 569

BLAST of CsaV3_3G008770 vs. NCBI nr
Match: XP_038876385.1 (mechanosensitive ion channel protein 1, mitochondrial-like [Benincasa hispida] >XP_038876386.1 mechanosensitive ion channel protein 1, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 902.5 bits (2331), Expect = 1.7e-258
Identity = 461/521 (88.48%), Postives = 491/521 (94.24%), Query Frame = 0

Query: 1   MACRRFAACSFQSFKPYTKISEYFDRVLRPNVVLNKRYYKDESHSFKRATVRGLHIPSYG 60
           MACRRFAACSFQSFKPYTKISEY DRVLRP+VVLNKRYY+DESHS K A+V  LHIPSYG
Sbjct: 1   MACRRFAACSFQSFKPYTKISEYVDRVLRPSVVLNKRYYRDESHSLKSASVCRLHIPSYG 60

Query: 61  THRIDTFLPASSMKYWNTIPLTCFGNLSNYRNYSSASGRKADISGDTGVPAASSGVEPDI 120
           THRIDTFLPASS+KYW+T PL   GNLSN R YSSAS RKAD+ GDTG PAAS+GVE DI
Sbjct: 61  THRIDTFLPASSVKYWSTTPLISLGNLSNSRFYSSASSRKADVPGDTG-PAASAGVEQDI 120

Query: 121 SNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPV 180
           SNPPD+GRDLL++VKDVWQSAVDAAS+TGQKAKEVSDELSPHVDKL DSHPYLKNVIVPV
Sbjct: 121 SNPPDLGRDLLDRVKDVWQSAVDAASYTGQKAKEVSDELSPHVDKLFDSHPYLKNVIVPV 180

Query: 181 SMTLGATLLAWLVMPRLLKRFHKYSMRSPVAIISGSLPSEEIPYEKSFWGALEDPLRYLV 240
           SMTL ATLLAW+VMPRLL+RFHKYSMRSPV+I+SGS+PS EIPYEKSFWGALEDPLRYLV
Sbjct: 181 SMTLTATLLAWVVMPRLLRRFHKYSMRSPVSILSGSIPSVEIPYEKSFWGALEDPLRYLV 240

Query: 241 TFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVLSRALATKTFAGLDR 300
           TFFAFSQIG++VAPTAVA EFVSQACRGAVILSLVWFIYRWKTNVLSRALA +TFAGLDR
Sbjct: 241 TFFAFSQIGMMVAPTAVAQEFVSQACRGAVILSLVWFIYRWKTNVLSRALAIQTFAGLDR 300

Query: 301 DRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNILSGLT 360
           DRLLT+DKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNILSGLT
Sbjct: 301 DRLLTIDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNILSGLT 360

Query: 361 MQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQW 420
           MQFSQPFSLGDTIKAGAIEGQVVEMGLM+T LLSAEKFP+VVPNSLFSSQVIINKSRAQW
Sbjct: 361 MQFSQPFSLGDTIKAGAIEGQVVEMGLMSTLLLSAEKFPIVVPNSLFSSQVIINKSRAQW 420

Query: 421 RAIVKRVPLQVDDISTVSQITDDIKNMLTSHPKVFLGKEAPYCFLSQIESTYAELTLGCN 480
           RAI K++PLQVDD STVS+I+D IKNML SHPKVFLGKE PYCFLSQIEST AELTLGCN
Sbjct: 421 RAITKKIPLQVDDFSTVSRISDGIKNMLMSHPKVFLGKEVPYCFLSQIESTCAELTLGCN 480

Query: 481 LRKMGKDESFSTEQDILLQSVQIIKAKGATLGSTMSDWTNK 522
           L+KMGK++ FSTEQDILLQSV+IIK  GATLGSTMSDWTNK
Sbjct: 481 LKKMGKEDLFSTEQDILLQSVRIIKENGATLGSTMSDWTNK 520

BLAST of CsaV3_3G008770 vs. ExPASy Swiss-Prot
Match: Q8VZL4 (Mechanosensitive ion channel protein 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MSL1 PE=1 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 6.4e-152
Identity = 270/432 (62.50%), Postives = 349/432 (80.79%), Query Frame = 0

Query: 89  NYRNYSSASGRKADISGDTGVPAASSGVEPDISNPPDVGRDLLEKVKDVWQSAVDAASFT 148
           NY  +SS +   +  S D G   AS        N    G D +EK KDV Q++VDA + T
Sbjct: 68  NYLGHSSYARAFSSKSDDFGSIVASGVTGSGDGN--GNGNDWVEKAKDVLQTSVDAVTET 127

Query: 149 GQKAKEVSDELSPHVDKLLDSHPYLKNVIVPVSMTLGATLLAWLVMPRLLKRFHKYSMRS 208
            +K K+VSDE+ PHV + LDS+PYLK+VIVPVS+T+  TL AW+VMPR+L+RFH Y+M+S
Sbjct: 128 AKKTKDVSDEMIPHVQQFLDSNPYLKDVIVPVSLTMTGTLFAWVVMPRILRRFHTYAMQS 187

Query: 209 PVAIISGSLPSEEIPYEKSFWGALEDPLRYLVTFFAFSQIGVIVAPTAVAPEFVSQACRG 268
              ++     +E++PYEKSFWGALEDP RYLVTF AF+QI  +VAPT +A ++ S   +G
Sbjct: 188 SAKLLPVGFSNEDVPYEKSFWGALEDPARYLVTFIAFAQIAAMVAPTTIAAQYFSPTVKG 247

Query: 269 AVILSLVWFIYRWKTNVLSRALATKTFAGLDRDRLLTLDKVSSVALFAIGLMALAEASGV 328
           AVILSLVWF+YRWKTNV++R L+ K+F GLDR+++LTLDKVSSV LFAIGLMA AEA GV
Sbjct: 248 AVILSLVWFLYRWKTNVITRMLSAKSFGGLDREKVLTLDKVSSVGLFAIGLMASAEACGV 307

Query: 329 AVQSMLTVGGIGGVATAFAARDILGNILSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLM 388
           AVQS+LTVGG+GGVATAFAARDILGN+LSGL+MQFS+PFS+GDTIKAG++EGQV+EMGL 
Sbjct: 308 AVQSILTVGGVGGVATAFAARDILGNVLSGLSMQFSRPFSMGDTIKAGSVEGQVIEMGLT 367

Query: 389 TTSLLSAEKFPVVVPNSLFSSQVIINKSRAQWRAIVKRVPLQVDDISTVSQITDDIKNML 448
           TTSLL+AEKFPV+VPNSLFSSQVI+NKSRAQWRAI  ++PLQ+DD+  + QI+++IK ML
Sbjct: 368 TTSLLNAEKFPVLVPNSLFSSQVIVNKSRAQWRAIASKIPLQIDDLDMIPQISNEIKEML 427

Query: 449 TSHPKVFLGKEAPYCFLSQIESTYAELTLGCNLRKMGKDESFSTEQDILLQSVQIIKAKG 508
            S+ KVFLGKEAP+C+LS++E ++AELT+GCNL +MGK+E ++T+Q++LL++V+IIK  G
Sbjct: 428 RSNTKVFLGKEAPHCYLSRVEKSFAELTIGCNLIRMGKEELYNTQQEVLLEAVKIIKKHG 487

Query: 509 ATLGSTMSDWTN 521
            +LG+T   W N
Sbjct: 488 VSLGTT---WDN 494

BLAST of CsaV3_3G008770 vs. ExPASy Swiss-Prot
Match: P0AEB6 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=83334 GN=ynaI PE=3 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.6e-22
Identity = 79/303 (26.07%), Postives = 146/303 (48.18%), Query Frame = 0

Query: 227 SFW---------GALEDPLRYLVTFFAFSQIGVIVAPTA-------VAPEFVSQACRG-- 286
           SFW         G L   +++L+     S +G I+           + P  +   C    
Sbjct: 27  SFWFRRGNRKRKGFLFHAVQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLI 86

Query: 287 AVILSLVWFIYRWKTNVLSRALATKTFAGLDRDRLLTLDKVSSVALFAIGLMALAEASGV 346
           AVIL++  F+      ++++    +   G D      + ++  + +  + ++   E  G+
Sbjct: 87  AVILTIKLFL------LINQFEKQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGM 146

Query: 347 AVQSMLTVGGIGGVATAFAARDILGNILSGLTMQFSQPFSLGDTIKA--GAIEGQVVEMG 406
           ++  +LT GGIGG+A   A +DIL N  SG+ + F +PFS+GD I++    IEG V E+G
Sbjct: 147 SLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIG 206

Query: 407 LMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQWRAIVKRVPLQVDDISTVSQITDDIKN 466
              T + + +  P+ VPNSLFSS  + N  R   R I   + L+ +D + V  I + ++ 
Sbjct: 207 WRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVRE 266

Query: 467 MLTSHPKVFLGKEAPYCFLSQIESTYAELTLGCNLRKMGKDESFSTEQDILLQSVQIIKA 510
           ML +HP +   ++    + +Q   +   + + C  +     E  + +QD+ L+ + I+++
Sbjct: 267 MLKNHPAID-QRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQS 322

BLAST of CsaV3_3G008770 vs. ExPASy Swiss-Prot
Match: P0AEB5 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) OX=83333 GN=ynaI PE=1 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.6e-22
Identity = 79/303 (26.07%), Postives = 146/303 (48.18%), Query Frame = 0

Query: 227 SFW---------GALEDPLRYLVTFFAFSQIGVIVAPTA-------VAPEFVSQACRG-- 286
           SFW         G L   +++L+     S +G I+           + P  +   C    
Sbjct: 27  SFWFRRGNRKRKGFLFHAVQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLI 86

Query: 287 AVILSLVWFIYRWKTNVLSRALATKTFAGLDRDRLLTLDKVSSVALFAIGLMALAEASGV 346
           AVIL++  F+      ++++    +   G D      + ++  + +  + ++   E  G+
Sbjct: 87  AVILTIKLFL------LINQFEKQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGM 146

Query: 347 AVQSMLTVGGIGGVATAFAARDILGNILSGLTMQFSQPFSLGDTIKA--GAIEGQVVEMG 406
           ++  +LT GGIGG+A   A +DIL N  SG+ + F +PFS+GD I++    IEG V E+G
Sbjct: 147 SLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIG 206

Query: 407 LMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQWRAIVKRVPLQVDDISTVSQITDDIKN 466
              T + + +  P+ VPNSLFSS  + N  R   R I   + L+ +D + V  I + ++ 
Sbjct: 207 WRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVRE 266

Query: 467 MLTSHPKVFLGKEAPYCFLSQIESTYAELTLGCNLRKMGKDESFSTEQDILLQSVQIIKA 510
           ML +HP +   ++    + +Q   +   + + C  +     E  + +QD+ L+ + I+++
Sbjct: 267 MLKNHPAID-QRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQS 322

BLAST of CsaV3_3G008770 vs. ExPASy Swiss-Prot
Match: O25170 (Uncharacterized MscS family protein HP_0415 OS=Helicobacter pylori (strain ATCC 700392 / 26695) OX=85962 GN=HP_0415 PE=3 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 1.3e-16
Identity = 76/304 (25.00%), Postives = 136/304 (44.74%), Query Frame = 0

Query: 238 YLVTFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFI---YRWKTNVLSRALATKT 297
           +L  F     + +   P A  P+ VS       I+ L W +   ++     L   +ATK+
Sbjct: 295 FLALFSCDVALDIFYYPNASPPK-VSMWVGAVYIMLLAWLVIALFKGYGEALVTNMATKS 354

Query: 298 FAGLDRDRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGN 357
                ++ +  + KV    +F + L+ + +  G  V +++   GIGG+A A A +D+L N
Sbjct: 355 THNFRKEVINLILKVVYFLIFIVALLGVLKQLGFNVSAIIASLGIGGLAVALAVKDVLAN 414

Query: 358 ILSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIIN 417
             + + +     FS GD I  G +EG VVEMGL  T++ + +   + VPNS  + + I N
Sbjct: 415 FFASVILLLDNSFSQGDWIVCGEVEGTVVEMGLRRTTIRAFDNALLSVPNSELAGKPIRN 474

Query: 418 KSRAQWRAIVKRVPLQV-----DDISTVSQITDDIKNMLTSHPKV--------------- 477
            SR   R + +R+ +++        S +     DIK ML +HPK+               
Sbjct: 475 WSR---RKVGRRIKMEIGLTYSSSQSALQLCVKDIKEMLENHPKIANGADSALQNVSDYR 534

Query: 478 ------------FLG-KEAPYCFLSQIESTYAELTLGCNLRKMGKDESFSTEQDILLQSV 506
                       FLG K   + FL Q   +   + + C  + +  +E    ++D++L+ +
Sbjct: 535 YMFKKDIVSIDDFLGYKNNLFVFLDQFADSSINILVYCFSKTVVWEEWLEVKEDVMLKIM 594

BLAST of CsaV3_3G008770 vs. ExPASy Swiss-Prot
Match: Q9ZKG7 (Uncharacterized MscS family protein jhp_0969 OS=Helicobacter pylori (strain J99 / ATCC 700824) OX=85963 GN=jhp_0969 PE=3 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.8e-16
Identity = 76/304 (25.00%), Postives = 136/304 (44.74%), Query Frame = 0

Query: 238 YLVTFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFI---YRWKTNVLSRALATKT 297
           +L  F     + +   P A  P+ VS       I+ L W +   ++     L   +ATK+
Sbjct: 295 FLALFSCDVALDIFYYPNASPPK-VSMWVGAVYIMLLAWLVIALFKGYGEALVTNVATKS 354

Query: 298 FAGLDRDRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGN 357
                ++ +  + KV    +F + L+ + +  G  V +++   GIGG+A A A +D+L N
Sbjct: 355 THNFRKEVINLILKVVYFLIFIVALLGVLKQLGFNVSAIIASLGIGGLAVALAVKDVLAN 414

Query: 358 ILSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIIN 417
             + + +     FS GD I  G +EG VVEMGL  T++ + +   + VPNS  + + I N
Sbjct: 415 FFASVILLLDNSFSQGDWIVCGEVEGTVVEMGLRRTTIRAFDNALLSVPNSELAGKPIRN 474

Query: 418 KSRAQWRAIVKRVPLQV-----DDISTVSQITDDIKNMLTSHPKV--------------- 477
            SR   R + +R+ +++        S +     DIK ML +HPK+               
Sbjct: 475 WSR---RKVGRRIKMEIGLTYSSSQSALQLCVKDIKEMLENHPKIANGADSALQNASDYR 534

Query: 478 ------------FLG-KEAPYCFLSQIESTYAELTLGCNLRKMGKDESFSTEQDILLQSV 506
                       FLG K   + FL Q   +   + + C  + +  +E    ++D++L+ +
Sbjct: 535 YMFKKDIVSIDDFLGYKNNLFVFLDQFADSSINILVYCFSKTVVWEEWLEVKEDVMLKIM 594

BLAST of CsaV3_3G008770 vs. ExPASy TrEMBL
Match: A0A0A0L685 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G121740 PE=3 SV=1)

HSP 1 Score: 1017.3 bits (2629), Expect = 2.3e-293
Identity = 521/521 (100.00%), Postives = 521/521 (100.00%), Query Frame = 0

Query: 1   MACRRFAACSFQSFKPYTKISEYFDRVLRPNVVLNKRYYKDESHSFKRATVRGLHIPSYG 60
           MACRRFAACSFQSFKPYTKISEYFDRVLRPNVVLNKRYYKDESHSFKRATVRGLHIPSYG
Sbjct: 1   MACRRFAACSFQSFKPYTKISEYFDRVLRPNVVLNKRYYKDESHSFKRATVRGLHIPSYG 60

Query: 61  THRIDTFLPASSMKYWNTIPLTCFGNLSNYRNYSSASGRKADISGDTGVPAASSGVEPDI 120
           THRIDTFLPASSMKYWNTIPLTCFGNLSNYRNYSSASGRKADISGDTGVPAASSGVEPDI
Sbjct: 61  THRIDTFLPASSMKYWNTIPLTCFGNLSNYRNYSSASGRKADISGDTGVPAASSGVEPDI 120

Query: 121 SNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPV 180
           SNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPV
Sbjct: 121 SNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPV 180

Query: 181 SMTLGATLLAWLVMPRLLKRFHKYSMRSPVAIISGSLPSEEIPYEKSFWGALEDPLRYLV 240
           SMTLGATLLAWLVMPRLLKRFHKYSMRSPVAIISGSLPSEEIPYEKSFWGALEDPLRYLV
Sbjct: 181 SMTLGATLLAWLVMPRLLKRFHKYSMRSPVAIISGSLPSEEIPYEKSFWGALEDPLRYLV 240

Query: 241 TFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVLSRALATKTFAGLDR 300
           TFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVLSRALATKTFAGLDR
Sbjct: 241 TFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVLSRALATKTFAGLDR 300

Query: 301 DRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNILSGLT 360
           DRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNILSGLT
Sbjct: 301 DRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNILSGLT 360

Query: 361 MQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQW 420
           MQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQW
Sbjct: 361 MQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQW 420

Query: 421 RAIVKRVPLQVDDISTVSQITDDIKNMLTSHPKVFLGKEAPYCFLSQIESTYAELTLGCN 480
           RAIVKRVPLQVDDISTVSQITDDIKNMLTSHPKVFLGKEAPYCFLSQIESTYAELTLGCN
Sbjct: 421 RAIVKRVPLQVDDISTVSQITDDIKNMLTSHPKVFLGKEAPYCFLSQIESTYAELTLGCN 480

Query: 481 LRKMGKDESFSTEQDILLQSVQIIKAKGATLGSTMSDWTNK 522
           LRKMGKDESFSTEQDILLQSVQIIKAKGATLGSTMSDWTNK
Sbjct: 481 LRKMGKDESFSTEQDILLQSVQIIKAKGATLGSTMSDWTNK 521

BLAST of CsaV3_3G008770 vs. ExPASy TrEMBL
Match: A0A1S3AVZ3 (mechanosensitive ion channel protein 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103483257 PE=3 SV=1)

HSP 1 Score: 972.2 bits (2512), Expect = 8.4e-280
Identity = 496/521 (95.20%), Postives = 507/521 (97.31%), Query Frame = 0

Query: 1   MACRRFAACSFQSFKPYTKISEYFDRVLRPNVVLNKRYYKDESHSFKRATVRGLHIPSYG 60
           MACRRFAACSFQSFKPYTKIS+Y DRVLRPNVVLNKRYY DESHSFKRATV GLHIPSYG
Sbjct: 1   MACRRFAACSFQSFKPYTKISKYVDRVLRPNVVLNKRYYNDESHSFKRATVCGLHIPSYG 60

Query: 61  THRIDTFLPASSMKYWNTIPLTCFGNLSNYRNYSSASGRKADISGDTGVPAASSGVEPDI 120
           THRIDTFLPASSMKYWNTIPLT  GNLSNYRNYSS SGRKADI+GDTGVPAAS+GVEPDI
Sbjct: 61  THRIDTFLPASSMKYWNTIPLTSIGNLSNYRNYSSTSGRKADITGDTGVPAASAGVEPDI 120

Query: 121 SNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPV 180
           SNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPV
Sbjct: 121 SNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPV 180

Query: 181 SMTLGATLLAWLVMPRLLKRFHKYSMRSPVAIISGSLPSEEIPYEKSFWGALEDPLRYLV 240
           SMTL  TLLAW VMPRLL+RFHKYSMRSPVAIISG+LPSEEIPYEKSFWGALEDPLRYLV
Sbjct: 181 SMTLAGTLLAWAVMPRLLRRFHKYSMRSPVAIISGALPSEEIPYEKSFWGALEDPLRYLV 240

Query: 241 TFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVLSRALATKTFAGLDR 300
           TFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNV SRALAT+T AGLDR
Sbjct: 241 TFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVFSRALATQTSAGLDR 300

Query: 301 DRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNILSGLT 360
           DRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARD+LGNILSGLT
Sbjct: 301 DRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDVLGNILSGLT 360

Query: 361 MQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQW 420
           MQFSQPFSLGDTIKAG IEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQW
Sbjct: 361 MQFSQPFSLGDTIKAGTIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQW 420

Query: 421 RAIVKRVPLQVDDISTVSQITDDIKNMLTSHPKVFLGKEAPYCFLSQIESTYAELTLGCN 480
           RAIVKRVPLQVDDISTVSQITDDIK ML SHPKVFLGKEAPYCFLSQIESTYAELT+GCN
Sbjct: 421 RAIVKRVPLQVDDISTVSQITDDIKKMLMSHPKVFLGKEAPYCFLSQIESTYAELTVGCN 480

Query: 481 LRKMGKDESFSTEQDILLQSVQIIKAKGATLGSTMSDWTNK 522
           L+KMGKDESFSTEQDILLQSVQIIKA+GATLGST+SDWTNK
Sbjct: 481 LKKMGKDESFSTEQDILLQSVQIIKARGATLGSTVSDWTNK 521

BLAST of CsaV3_3G008770 vs. ExPASy TrEMBL
Match: A0A5A7U5P8 (Mechanosensitive ion channel protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G001760 PE=3 SV=1)

HSP 1 Score: 963.4 bits (2489), Expect = 3.9e-277
Identity = 492/517 (95.16%), Postives = 503/517 (97.29%), Query Frame = 0

Query: 4   RRFAACSFQSFKPYTKISEYFDRVLRPNVVLNKRYYKDESHSFKRATVRGLHIPSYGTHR 63
           RRFAACSFQSFKPYTKIS+Y DRVLRPNVVLNKRYY DESHSFKRATV GLHIPSYGTHR
Sbjct: 35  RRFAACSFQSFKPYTKISKYVDRVLRPNVVLNKRYYNDESHSFKRATVCGLHIPSYGTHR 94

Query: 64  IDTFLPASSMKYWNTIPLTCFGNLSNYRNYSSASGRKADISGDTGVPAASSGVEPDISNP 123
           IDTFLPASSMKYWNTIPLT  GNLSNYRNYSS SGRKADI+GDTGVPAAS+GVEPDISNP
Sbjct: 95  IDTFLPASSMKYWNTIPLTSIGNLSNYRNYSSTSGRKADITGDTGVPAASAGVEPDISNP 154

Query: 124 PDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPVSMT 183
           PDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPVSMT
Sbjct: 155 PDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPVSMT 214

Query: 184 LGATLLAWLVMPRLLKRFHKYSMRSPVAIISGSLPSEEIPYEKSFWGALEDPLRYLVTFF 243
           L  TLLAW VMPRLL+RFHKYSMRSPVAIISG+LPSEEIPYEKSFWGALEDPLRYLVTFF
Sbjct: 215 LAGTLLAWAVMPRLLRRFHKYSMRSPVAIISGALPSEEIPYEKSFWGALEDPLRYLVTFF 274

Query: 244 AFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVLSRALATKTFAGLDRDRL 303
           AFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNV SRALAT+T AGLDRDRL
Sbjct: 275 AFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVFSRALATQTSAGLDRDRL 334

Query: 304 LTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNILSGLTMQF 363
           LTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARD+LGNILSGLTMQF
Sbjct: 335 LTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDVLGNILSGLTMQF 394

Query: 364 SQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQWRAI 423
           SQPFSLGDTIKAG IEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQWRAI
Sbjct: 395 SQPFSLGDTIKAGTIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQWRAI 454

Query: 424 VKRVPLQVDDISTVSQITDDIKNMLTSHPKVFLGKEAPYCFLSQIESTYAELTLGCNLRK 483
           VKRVPLQVDDISTVSQITDDIK ML SHPKVFLGKEAPYCFLSQIESTYAELT+GCNL+K
Sbjct: 455 VKRVPLQVDDISTVSQITDDIKKMLMSHPKVFLGKEAPYCFLSQIESTYAELTVGCNLKK 514

Query: 484 MGKDESFSTEQDILLQSVQIIKAKGATLGSTMSDWTN 521
           MGKDESFSTEQDILLQSVQIIKA+GATLGST+SDWTN
Sbjct: 515 MGKDESFSTEQDILLQSVQIIKARGATLGSTVSDWTN 551

BLAST of CsaV3_3G008770 vs. ExPASy TrEMBL
Match: A0A5D3D1A1 (Mechanosensitive ion channel protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004820 PE=3 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 3.3e-276
Identity = 490/516 (94.96%), Postives = 502/516 (97.29%), Query Frame = 0

Query: 5   RFAACSFQSFKPYTKISEYFDRVLRPNVVLNKRYYKDESHSFKRATVRGLHIPSYGTHRI 64
           RFAACSFQSFKPYT IS+Y DRVLRPNVVLNKRYYKDESHSFKRATV GLHIP+YGTHRI
Sbjct: 54  RFAACSFQSFKPYTNISKYVDRVLRPNVVLNKRYYKDESHSFKRATVCGLHIPNYGTHRI 113

Query: 65  DTFLPASSMKYWNTIPLTCFGNLSNYRNYSSASGRKADISGDTGVPAASSGVEPDISNPP 124
           DTFLPASSMKYWNTIPLT  GNLSNYRNYSS SGRKADI+GDTGVPAAS+GVEPDISNPP
Sbjct: 114 DTFLPASSMKYWNTIPLTSIGNLSNYRNYSSTSGRKADITGDTGVPAASAGVEPDISNPP 173

Query: 125 DVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPVSMTL 184
           DVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPVSMTL
Sbjct: 174 DVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVPVSMTL 233

Query: 185 GATLLAWLVMPRLLKRFHKYSMRSPVAIISGSLPSEEIPYEKSFWGALEDPLRYLVTFFA 244
             TLLAW VMPRLL+RFHKYSMRSPVAIISG+LPSEEIPYEKSFWGALEDPLRYLVTFFA
Sbjct: 234 AGTLLAWAVMPRLLRRFHKYSMRSPVAIISGALPSEEIPYEKSFWGALEDPLRYLVTFFA 293

Query: 245 FSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVLSRALATKTFAGLDRDRLL 304
           FSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNV SRALAT+T AGLDRDRLL
Sbjct: 294 FSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVFSRALATQTSAGLDRDRLL 353

Query: 305 TLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNILSGLTMQFS 364
           TLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARD+LGNILSGLTMQFS
Sbjct: 354 TLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDVLGNILSGLTMQFS 413

Query: 365 QPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQWRAIV 424
           QPFSLGDTIKAG IEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQWRAIV
Sbjct: 414 QPFSLGDTIKAGTIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQWRAIV 473

Query: 425 KRVPLQVDDISTVSQITDDIKNMLTSHPKVFLGKEAPYCFLSQIESTYAELTLGCNLRKM 484
           KRVPLQVDDISTVSQITDDIK ML SHPKVFLGKEAPYCFLSQIESTYAELT+GCNL+KM
Sbjct: 474 KRVPLQVDDISTVSQITDDIKKMLMSHPKVFLGKEAPYCFLSQIESTYAELTVGCNLKKM 533

Query: 485 GKDESFSTEQDILLQSVQIIKAKGATLGSTMSDWTN 521
           GKDESFSTEQDILLQSVQIIKA+GATLGST+SDWTN
Sbjct: 534 GKDESFSTEQDILLQSVQIIKARGATLGSTVSDWTN 569

BLAST of CsaV3_3G008770 vs. ExPASy TrEMBL
Match: A0A6J1I1Q4 (mechanosensitive ion channel protein 1, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111468640 PE=3 SV=1)

HSP 1 Score: 820.1 bits (2117), Expect = 5.3e-234
Identity = 422/514 (82.10%), Postives = 457/514 (88.91%), Query Frame = 0

Query: 9   CSFQSFKPYTKISEYFDRVLRPNVVLNKRYYKDESHSFKRATVRG---------LHIPSY 68
           CSFQ  K YTKISEY +RVLRP+VVLNK+YYKDESHS K  +  G         LH PSY
Sbjct: 20  CSFQYCKSYTKISEYVNRVLRPSVVLNKQYYKDESHSLKNVSRTGFIRAPSVCRLHTPSY 79

Query: 69  GTHRIDTFLPASSMKYWNTIPLTCFGNLSNYRNYSSASGRKADISGDTGVPAASSGVEPD 128
           GTH I TF PAS +KYWNTIP T    LSN R YSS S RKADI+GDTGVP ASSGVE D
Sbjct: 80  GTHPIATFSPASPVKYWNTIPPTSLAYLSNPRFYSSTSSRKADITGDTGVPLASSGVEAD 139

Query: 129 ISNPPDVGRDLLEKVKDVWQSAVDAASFTGQKAKEVSDELSPHVDKLLDSHPYLKNVIVP 188
           ISNPPDVGRD LE+VKD WQSAVDAASFTGQKA+EVSDE SPHVDK+LDSHPYLKN I P
Sbjct: 140 ISNPPDVGRDWLERVKDGWQSAVDAASFTGQKAREVSDEFSPHVDKILDSHPYLKNEIAP 199

Query: 189 VSMTLGATLLAWLVMPRLLKRFHKYSMRSPVAIISGSLPSEEIPYEKSFWGALEDPLRYL 248
           V MTL ATLLAW+VMPRLL+RFHKYSMRSPVAI+SGS   EEIPYEKSFWGALEDPLRYL
Sbjct: 200 VGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIEEIPYEKSFWGALEDPLRYL 259

Query: 249 VTFFAFSQIGVIVAPTAVAPEFVSQACRGAVILSLVWFIYRWKTNVLSRALATKTFAGLD 308
           VTF+AFSQIG +VAPTA+A EFVSQACRGAVILSLVWFIYRWKTNV SRALA++ F+GLD
Sbjct: 260 VTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYRWKTNVFSRALASQNFSGLD 319

Query: 309 RDRLLTLDKVSSVALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNILSGL 368
           R++LLTLDKVSSV LFAIGLMALAEASGVAVQSMLTVGGIGGVATAFA+RDILGN+LSGL
Sbjct: 320 REKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGGVATAFASRDILGNVLSGL 379

Query: 369 TMQFSQPFSLGDTIKAGAIEGQVVEMGLMTTSLLSAEKFPVVVPNSLFSSQVIINKSRAQ 428
           TMQFSQPFSLGDTIKAGAIEGQV+EMGLMTTSLLSAEKFP+VVPNSLFSSQVIINKSRAQ
Sbjct: 380 TMQFSQPFSLGDTIKAGAIEGQVIEMGLMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQ 439

Query: 429 WRAIVKRVPLQVDDISTVSQITDDIKNMLTSHPKVFLGKEAPYCFLSQIESTYAELTLGC 488
           WRAI K++P QV D STVS+I+DDIK+ML SHPKVFLGKEAPYCFLSQIES+YAELT+GC
Sbjct: 440 WRAITKKIPFQVADFSTVSKISDDIKSMLRSHPKVFLGKEAPYCFLSQIESSYAELTIGC 499

Query: 489 NLRKMGKDESFSTEQDILLQSVQIIKAKGATLGS 514
           NL++MGK+E FSTEQDILLQSVQIIK  GATLG+
Sbjct: 500 NLKRMGKEELFSTEQDILLQSVQIIKENGATLGN 533

BLAST of CsaV3_3G008770 vs. TAIR 10
Match: AT4G00290.1 (Mechanosensitive ion channel protein )

HSP 1 Score: 538.9 bits (1387), Expect = 4.6e-153
Identity = 270/432 (62.50%), Postives = 349/432 (80.79%), Query Frame = 0

Query: 89  NYRNYSSASGRKADISGDTGVPAASSGVEPDISNPPDVGRDLLEKVKDVWQSAVDAASFT 148
           NY  +SS +   +  S D G   AS        N    G D +EK KDV Q++VDA + T
Sbjct: 68  NYLGHSSYARAFSSKSDDFGSIVASGVTGSGDGN--GNGNDWVEKAKDVLQTSVDAVTET 127

Query: 149 GQKAKEVSDELSPHVDKLLDSHPYLKNVIVPVSMTLGATLLAWLVMPRLLKRFHKYSMRS 208
            +K K+VSDE+ PHV + LDS+PYLK+VIVPVS+T+  TL AW+VMPR+L+RFH Y+M+S
Sbjct: 128 AKKTKDVSDEMIPHVQQFLDSNPYLKDVIVPVSLTMTGTLFAWVVMPRILRRFHTYAMQS 187

Query: 209 PVAIISGSLPSEEIPYEKSFWGALEDPLRYLVTFFAFSQIGVIVAPTAVAPEFVSQACRG 268
              ++     +E++PYEKSFWGALEDP RYLVTF AF+QI  +VAPT +A ++ S   +G
Sbjct: 188 SAKLLPVGFSNEDVPYEKSFWGALEDPARYLVTFIAFAQIAAMVAPTTIAAQYFSPTVKG 247

Query: 269 AVILSLVWFIYRWKTNVLSRALATKTFAGLDRDRLLTLDKVSSVALFAIGLMALAEASGV 328
           AVILSLVWF+YRWKTNV++R L+ K+F GLDR+++LTLDKVSSV LFAIGLMA AEA GV
Sbjct: 248 AVILSLVWFLYRWKTNVITRMLSAKSFGGLDREKVLTLDKVSSVGLFAIGLMASAEACGV 307

Query: 329 AVQSMLTVGGIGGVATAFAARDILGNILSGLTMQFSQPFSLGDTIKAGAIEGQVVEMGLM 388
           AVQS+LTVGG+GGVATAFAARDILGN+LSGL+MQFS+PFS+GDTIKAG++EGQV+EMGL 
Sbjct: 308 AVQSILTVGGVGGVATAFAARDILGNVLSGLSMQFSRPFSMGDTIKAGSVEGQVIEMGLT 367

Query: 389 TTSLLSAEKFPVVVPNSLFSSQVIINKSRAQWRAIVKRVPLQVDDISTVSQITDDIKNML 448
           TTSLL+AEKFPV+VPNSLFSSQVI+NKSRAQWRAI  ++PLQ+DD+  + QI+++IK ML
Sbjct: 368 TTSLLNAEKFPVLVPNSLFSSQVIVNKSRAQWRAIASKIPLQIDDLDMIPQISNEIKEML 427

Query: 449 TSHPKVFLGKEAPYCFLSQIESTYAELTLGCNLRKMGKDESFSTEQDILLQSVQIIKAKG 508
            S+ KVFLGKEAP+C+LS++E ++AELT+GCNL +MGK+E ++T+Q++LL++V+IIK  G
Sbjct: 428 RSNTKVFLGKEAPHCYLSRVEKSFAELTIGCNLIRMGKEELYNTQQEVLLEAVKIIKKHG 487

Query: 509 ATLGSTMSDWTN 521
            +LG+T   W N
Sbjct: 488 VSLGTT---WDN 494

BLAST of CsaV3_3G008770 vs. TAIR 10
Match: AT4G00234.1 (BEST Arabidopsis thaliana protein match is: Mechanosensitive ion channel protein (TAIR:AT4G00290.1); Has 57 Blast hits to 57 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 56; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 276.6 bits (706), Expect = 4.2e-74
Identity = 145/249 (58.23%), Postives = 186/249 (74.70%), Query Frame = 0

Query: 89  NYRNYSSASGRKADISGDTGVPAASSGVEPDISNPPDVGRDLLEKVKDVWQSAVDAASFT 148
           NY ++SS +   +  S D G   AS        N    G D +EK KDV Q++V+A + T
Sbjct: 18  NYLSHSSYARAFSSKSDDFGSIVASGVTGSGDGN----GNDWVEKAKDVVQTSVNAVAET 77

Query: 149 GQKAKEVSDELSPHVDKLLDSHPYLKNVIVPVSMTLGATLLAWLVMPRLLKRFHKYSMRS 208
            +K K+VSDE+ PHV + LDS+PYLK+VIVPVS+T+  TL AW+VMPR+L+RFH Y+M+S
Sbjct: 78  AKKTKDVSDEMIPHVQQFLDSNPYLKDVIVPVSLTVTGTLFAWIVMPRILRRFHTYAMQS 137

Query: 209 PVAIISGSLPSEEIPYEKSFWGALEDPLRYLVTFFAFSQIGVIVAPTAVAPEFVSQACRG 268
              ++     +E+IPYEKSFWGALEDP RYLVTF AF+QI  +VAPT +A ++ S   +G
Sbjct: 138 SAKLLPVGFSNEDIPYEKSFWGALEDPARYLVTFIAFAQIAAMVAPTTIAAQYFSPTVKG 197

Query: 269 AVILSLVWFIYRWKTNVLSRALATKTFAGLDRDRLLTLDKVSSVALFAIGLMALAEASGV 328
           AVILS+VWF+YRWKTNV++R L+ K+F GLDRD++LTLDKVSSV LFAIGLMA AEA GV
Sbjct: 198 AVILSVVWFLYRWKTNVITRMLSAKSFGGLDRDKVLTLDKVSSVGLFAIGLMASAEACGV 257

Query: 329 AVQSMLTVG 338
           AVQS+LTVG
Sbjct: 258 AVQSILTVG 262

BLAST of CsaV3_3G008770 vs. TAIR 10
Match: AT1G58200.1 (MSCS-like 3 )

HSP 1 Score: 72.0 bits (175), Expect = 1.6e-12
Identity = 54/198 (27.27%), Postives = 99/198 (50.00%), Query Frame = 0

Query: 324 EASGVAVQSMLTVGGIGGVATAFAARDILGNILSGLTMQFSQPFSLGDTI--KAGAIE-- 383
           E  G + Q  LT GG+G V    A R+IL N LS + +  ++PF L + I  K G  E  
Sbjct: 257 ELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIMIHATRPFVLNEWIQTKIGGYEVS 316

Query: 384 GQVVEMGLMTTSLLSA-EKFPVVVPNSLFSSQVIIN-KSRAQWRAIVKRVPLQVDDISTV 443
           G V ++G  + +++   ++  V +PN  FS  ++ N   +  WR I   + +   D+S +
Sbjct: 317 GTVEQVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTHWR-IKTHLAISHLDVSKI 376

Query: 444 SQITDDIKNMLTSHPKVFLGKEAPYCFLSQI--ESTYAELTLGCNLRKMGKDESFSTEQD 503
           + I  D++ +L+ +P++   K     FL  I  E+    + + C ++    +E    ++ 
Sbjct: 377 NNIVADMRKVLSKNPQIEQQKIHRRVFLEDIDPENQALRILISCFVKTSRFEEYLCVKEA 436

Query: 504 ILLQSVQIIKAKGATLGS 514
           +LL  + +I+  GA L +
Sbjct: 437 VLLDLLTVIRHHGARLAT 453

BLAST of CsaV3_3G008770 vs. TAIR 10
Match: AT1G58200.2 (MSCS-like 3 )

HSP 1 Score: 72.0 bits (175), Expect = 1.6e-12
Identity = 54/198 (27.27%), Postives = 99/198 (50.00%), Query Frame = 0

Query: 324 EASGVAVQSMLTVGGIGGVATAFAARDILGNILSGLTMQFSQPFSLGDTI--KAGAIE-- 383
           E  G + Q  LT GG+G V    A R+IL N LS + +  ++PF L + I  K G  E  
Sbjct: 257 ELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIMIHATRPFVLNEWIQTKIGGYEVS 316

Query: 384 GQVVEMGLMTTSLLSA-EKFPVVVPNSLFSSQVIIN-KSRAQWRAIVKRVPLQVDDISTV 443
           G V ++G  + +++   ++  V +PN  FS  ++ N   +  WR I   + +   D+S +
Sbjct: 317 GTVEQVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTHWR-IKTHLAISHLDVSKI 376

Query: 444 SQITDDIKNMLTSHPKVFLGKEAPYCFLSQI--ESTYAELTLGCNLRKMGKDESFSTEQD 503
           + I  D++ +L+ +P++   K     FL  I  E+    + + C ++    +E    ++ 
Sbjct: 377 NNIVADMRKVLSKNPQIEQQKIHRRVFLEDIDPENQALRILISCFVKTSRFEEYLCVKEA 436

Query: 504 ILLQSVQIIKAKGATLGS 514
           +LL  + +I+  GA L +
Sbjct: 437 VLLDLLTVIRHHGARLAT 453

BLAST of CsaV3_3G008770 vs. TAIR 10
Match: AT5G10490.1 (MSCS-like 2 )

HSP 1 Score: 64.3 bits (155), Expect = 3.3e-10
Identity = 50/209 (23.92%), Postives = 98/209 (46.89%), Query Frame = 0

Query: 313 ALFAIGLMALAEASGVAVQSMLTVGGIGGVATAFAARDILGNILSGLTMQFSQPFSLGD- 372
           A++   +    E  G + Q  LT GG+G V    A R+IL N LS + +  ++PF L + 
Sbjct: 244 AVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVLNEW 303

Query: 373 ---TIKAGAIEGQVVEMGLMTTSLLSAE-KFPVVVPNSLFSSQVIIN-KSRAQWRAIVKR 432
               I+   + G V  +G  + +++  E +  + +PN  F+  V+ N   +  WR I   
Sbjct: 304 IQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWR-IKTH 363

Query: 433 VPLQVDDISTVSQITDDIKNMLTSHPKVFLGKEAPYCFLSQI--ESTYAELTLGCNLRKM 492
           + +   D++ ++ I  D++ +L  +P V   +     FL  +  E+    + + C ++  
Sbjct: 364 LAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALSILISCFVKTS 423

Query: 493 GKDESFSTEQDILLQSVQIIKAKGATLGS 514
             +E    ++ ILL  +++I    A L +
Sbjct: 424 HHEEYLGVKEAILLDLLRVISHHRARLAT 451

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004133856.14.7e-293100.00mechanosensitive ion channel protein 1, mitochondrial isoform X1 [Cucumis sativu... [more]
XP_008438011.11.7e-27995.20PREDICTED: mechanosensitive ion channel protein 1, mitochondrial [Cucumis melo] ... [more]
KAA0048955.18.1e-27795.16mechanosensitive ion channel protein 1 [Cucumis melo var. makuwa][more]
TYK17612.16.8e-27694.96mechanosensitive ion channel protein 1 [Cucumis melo var. makuwa][more]
XP_038876385.11.7e-25888.48mechanosensitive ion channel protein 1, mitochondrial-like [Benincasa hispida] >... [more]
Match NameE-valueIdentityDescription
Q8VZL46.4e-15262.50Mechanosensitive ion channel protein 1, mitochondrial OS=Arabidopsis thaliana OX... [more]
P0AEB61.6e-2226.07Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=833... [more]
P0AEB51.6e-2226.07Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) O... [more]
O251701.3e-1625.00Uncharacterized MscS family protein HP_0415 OS=Helicobacter pylori (strain ATCC ... [more]
Q9ZKG71.8e-1625.00Uncharacterized MscS family protein jhp_0969 OS=Helicobacter pylori (strain J99 ... [more]
Match NameE-valueIdentityDescription
A0A0A0L6852.3e-293100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G121740 PE=3 SV=1[more]
A0A1S3AVZ38.4e-28095.20mechanosensitive ion channel protein 1, mitochondrial OS=Cucumis melo OX=3656 GN... [more]
A0A5A7U5P83.9e-27795.16Mechanosensitive ion channel protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A5D3D1A13.3e-27694.96Mechanosensitive ion channel protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1I1Q45.3e-23482.10mechanosensitive ion channel protein 1, mitochondrial-like OS=Cucurbita maxima O... [more]
Match NameE-valueIdentityDescription
AT4G00290.14.6e-15362.50Mechanosensitive ion channel protein [more]
AT4G00234.14.2e-7458.23BEST Arabidopsis thaliana protein match is: Mechanosensitive ion channel protein... [more]
AT1G58200.11.6e-1227.27MSCS-like 3 [more]
AT1G58200.21.6e-1227.27MSCS-like 3 [more]
AT5G10490.13.3e-1023.92MSCS-like 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 308..508
e-value: 1.1E-34
score: 119.8
NoneNo IPR availableGENE3D1.10.287.1260coord: 262..364
e-value: 2.3E-14
score: 55.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 98..124
NoneNo IPR availablePANTHERPTHR30566:SF5MECHANOSENSITIVE ION CHANNEL PROTEIN 1, MITOCHONDRIAL-RELATEDcoord: 69..517
NoneNo IPR availablePANTHERPTHR30566YNAI-RELATED MECHANOSENSITIVE ION CHANNELcoord: 69..517
IPR023408Mechanosensitive ion channel MscS domain superfamilyGENE3D2.30.30.60coord: 366..414
e-value: 4.3E-12
score: 47.8
IPR011014Mechanosensitive ion channel MscS, transmembrane-2SUPERFAMILY82861Mechanosensitive channel protein MscS (YggB), transmembrane regioncoord: 267..349
IPR010920LSM domain superfamilySUPERFAMILY50182Sm-like ribonucleoproteinscoord: 350..415

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_3G008770.1CsaV3_3G008770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016020 membrane