CsaV3_3G007340 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_3G007340 vs. NCBI nr
Match: XP_011650623.1 (vacuolar protein sorting-associated protein 8 homolog [Cucumis sativus] >KGN56350.1 hypothetical protein Csa_010564 [Cucumis sativus])

HSP 1 Score: 3843.1 bits (9965), Expect = 0.0e+00
Identity = 1960/1960 (100.00%), Postives = 1960/1960 (100.00%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSSGGDDDDDDDLSFPHRSIDEILNDSSSSTSPSP 60
            MTEELTDTETLPPMELDLNAFIHAHLSSGGDDDDDDDLSFPHRSIDEILNDSSSSTSPSP
Sbjct: 1    MTEELTDTETLPPMELDLNAFIHAHLSSGGDDDDDDDLSFPHRSIDEILNDSSSSTSPSP 60

Query: 61   SSSPHFPPPRGRRNIVAGDDGVSASPSTSPYKDSEAARNNPWNEKSAQLKPGTASHSKVG 120
            SSSPHFPPPRGRRNIVAGDDGVSASPSTSPYKDSEAARNNPWNEKSAQLKPGTASHSKVG
Sbjct: 61   SSSPHFPPPRGRRNIVAGDDGVSASPSTSPYKDSEAARNNPWNEKSAQLKPGTASHSKVG 120

Query: 121  ELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRRAGYGNMV 180
            ELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRRAGYGNMV
Sbjct: 121  ELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRRAGYGNMV 180

Query: 181  LDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNVSGELWATN 240
            LDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNVSGELWATN
Sbjct: 181  LDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNVSGELWATN 240

Query: 241  NIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGPAEKNVCST 300
            NIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGPAEKNVCST
Sbjct: 241  NIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGPAEKNVCST 300

Query: 301  DAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSKDTEVDLAIED 360
            DAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSKDTEVDLAIED
Sbjct: 301  DAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSKDTEVDLAIED 360

Query: 361  PSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFTALHWEEGVAA 420
            PSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFTALHWEEGVAA
Sbjct: 361  PSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFTALHWEEGVAA 420

Query: 421  QPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYIAVGMSKGN 480
            QPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYIAVGMSKGN
Sbjct: 421  QPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYIAVGMSKGN 480

Query: 481  IVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGYSDGHITVWDV 540
            IVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGYSDGHITVWDV
Sbjct: 481  IVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGYSDGHITVWDV 540

Query: 541  LRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSS 600
            LRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSS
Sbjct: 541  LRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSS 600

Query: 601  KTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVVGGDSGWKL 660
            KTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVVGGDSGWKL
Sbjct: 601  KTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVVGGDSGWKL 660

Query: 661  FNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYTAWKCSQSFET 720
            FNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYTAWKCSQSFET
Sbjct: 661  FNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYTAWKCSQSFET 720

Query: 721  SPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDDQVLVILTVTG 780
            SPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDDQVLVILTVTG
Sbjct: 721  SPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDDQVLVILTVTG 780

Query: 781  QLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVAVRGASIYVLG 840
            QLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVAVRGASIYVLG
Sbjct: 781  QLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVAVRGASIYVLG 840

Query: 841  PMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQELVMPF 900
            PMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQELVMPF
Sbjct: 841  PMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQELVMPF 900

Query: 901  LIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVGGVAVEFCV 960
            LIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVGGVAVEFCV
Sbjct: 901  LIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVGGVAVEFCV 960

Query: 961  HISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQ 1020
            HISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQ
Sbjct: 961  HISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQ 1020

Query: 1021 RVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLRTSKSK 1080
            RVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLRTSKSK
Sbjct: 1021 RVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLRTSKSK 1080

Query: 1081 HASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVDTRSISNK 1140
            HASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVDTRSISNK
Sbjct: 1081 HASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVDTRSISNK 1140

Query: 1141 SSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQEEKNSISGR 1200
            SSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQEEKNSISGR
Sbjct: 1141 SSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQEEKNSISGR 1200

Query: 1201 KNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIATYVACGKA 1260
            KNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIATYVACGKA
Sbjct: 1201 KNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIATYVACGKA 1260

Query: 1261 TVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVIPETHWNPS 1320
            TVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVIPETHWNPS
Sbjct: 1261 TVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVIPETHWNPS 1320

Query: 1321 SVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLELGNSEQTE 1380
            SVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLELGNSEQTE
Sbjct: 1321 SVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLELGNSEQTE 1380

Query: 1381 FRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKTLIEVHLSG 1440
            FRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKTLIEVHLSG
Sbjct: 1381 FRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKTLIEVHLSG 1440

Query: 1441 SPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDIIELYVELLCQHER 1500
            SPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDIIELYVELLCQHER
Sbjct: 1441 SPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDIIELYVELLCQHER 1500

Query: 1501 ESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFHDLE 1560
            ESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFHDLE
Sbjct: 1501 ESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFHDLE 1560

Query: 1561 AAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLNSEESQTLW 1620
            AAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLNSEESQTLW
Sbjct: 1561 AAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLNSEESQTLW 1620

Query: 1621 FKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKSNKVAHLLR 1680
            FKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKSNKVAHLLR
Sbjct: 1621 FKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKSNKVAHLLR 1680

Query: 1681 KLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGFERRILDSA 1740
            KLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGFERRILDSA
Sbjct: 1681 KLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGFERRILDSA 1740

Query: 1741 KALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCGHATHLQCE 1800
            KALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCGHATHLQCE
Sbjct: 1741 KALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCGHATHLQCE 1800

Query: 1801 DLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVSYPQETDLL 1860
            DLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVSYPQETDLL
Sbjct: 1801 DLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVSYPQETDLL 1860

Query: 1861 ELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLLVGESSGGR 1920
            ELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLLVGESSGGR
Sbjct: 1861 ELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLLVGESSGGR 1920

Query: 1921 EKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1961
            EKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS
Sbjct: 1921 EKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1960

BLAST of CsaV3_3G007340 vs. NCBI nr
Match: XP_008437780.1 (PREDICTED: vacuolar protein sorting-associated protein 8 homolog [Cucumis melo])

HSP 1 Score: 3608.5 bits (9356), Expect = 0.0e+00
Identity = 1856/1968 (94.31%), Postives = 1887/1968 (95.88%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSSGGDDDDDDDLSFPHRSIDEILNDSSSSTSPSP 60
            MTEELTDT TLPPMELDLNAFIHAHLSS GDDDDDDDLSFPHRSIDEILNDSSSSTS SP
Sbjct: 1    MTEELTDTRTLPPMELDLNAFIHAHLSS-GDDDDDDDLSFPHRSIDEILNDSSSSTSSSP 60

Query: 61   SSSPHFPPPRGRRNIVAGDDGVSASPSTSPY--------KDSEAARNNPWNEKSAQLKPG 120
            SSSPH PP RGRRNIVAG+ GVSASPSTSP+        KDSEA RNNPWNEKSAQ KPG
Sbjct: 61   SSSPHSPPSRGRRNIVAGNGGVSASPSTSPFKSLLEETIKDSEAPRNNPWNEKSAQSKPG 120

Query: 121  TASHSKVGELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSR 180
              SHSK+GELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSR
Sbjct: 121  KVSHSKIGELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSR 180

Query: 181  RAGYGNMVLDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNV 240
            RAGYGNM LDDDELASSSAVDSEF SDSLYH NIH KESGENSISVVDRITDYQ+AS +V
Sbjct: 181  RAGYGNMALDDDELASSSAVDSEFLSDSLYHTNIHLKESGENSISVVDRITDYQVASRDV 240

Query: 241  SGELWATNNIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGP 300
            S ELW  NNIRD VPHNDEFRMTEDMEFEAE SSVDDVNF ESL+TVPP ETNDRSLLGP
Sbjct: 241  S-ELWDRNNIRDSVPHNDEFRMTEDMEFEAEPSSVDDVNFNESLTTVPPAETNDRSLLGP 300

Query: 301  AEKNVCSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSKDT 360
            AEKNVCSTDAHPTELDVDESNEGAIPR TEPDDE SAVGYGS ELETQDFEKYHQPSKDT
Sbjct: 301  AEKNVCSTDAHPTELDVDESNEGAIPRSTEPDDEGSAVGYGSPELETQDFEKYHQPSKDT 360

Query: 361  EVDLAIEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFTAL 420
            EVDLAIEDPSIVNDIIESGETTEQ DNLQIGK PE + VSSTNPL+LAEEIEKKQAFTAL
Sbjct: 361  EVDLAIEDPSIVNDIIESGETTEQLDNLQIGKHPETMPVSSTNPLELAEEIEKKQAFTAL 420

Query: 421  HWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYI 480
            HWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYI
Sbjct: 421  HWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYI 480

Query: 481  AVGMSKGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGYSD 540
            AVGMSKG+IVVVASKYSAQNGDNMDAKMILLGSQGDKSTAP TSLCFSQQ DLLLAGYSD
Sbjct: 481  AVGMSKGSIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPVTSLCFSQQADLLLAGYSD 540

Query: 541  GHITVWDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVV 600
            GHITVWDVLRASAAKVISGEH SPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVV
Sbjct: 541  GHITVWDVLRASAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVV 600

Query: 601  PLLNRFSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVV 660
            PLLNRFS KTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVV
Sbjct: 601  PLLNRFSIKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVV 660

Query: 661  GGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYTAW 720
            GGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSP+VEVYAQLSKPDGIREGSMPYTAW
Sbjct: 661  GGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPSVEVYAQLSKPDGIREGSMPYTAW 720

Query: 721  KCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDDQV 780
            KCSQSFETS SEAVERVSLLAIAWDKMVQVAKLVKTELKVCG WSLESAAIGVVWLDDQV
Sbjct: 721  KCSQSFETSSSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGNWSLESAAIGVVWLDDQV 780

Query: 781  LVILTVTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVAVR 840
            LVILTVTGQLFLFEKDGTMIHQTS+F DGFVKEDFIAYHTHFAN+LG+PEKAYHNCVAVR
Sbjct: 781  LVILTVTGQLFLFEKDGTMIHQTSVFADGFVKEDFIAYHTHFANVLGHPEKAYHNCVAVR 840

Query: 841  GASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLES 900
            GASIYVLGP HLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLES
Sbjct: 841  GASIYVLGPTHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLES 900

Query: 901  LQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVG 960
            LQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDD+T E  SAHSEIKEQYNRVG
Sbjct: 901  LQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDVTSERDSAHSEIKEQYNRVG 960

Query: 961  GVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEH 1020
            GVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEH
Sbjct: 961  GVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEH 1020

Query: 1021 YSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLA 1080
            YSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLA
Sbjct: 1021 YSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLA 1080

Query: 1081 VLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAV 1140
            VLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAV
Sbjct: 1081 VLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAV 1140

Query: 1141 DTRSISNKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQE 1200
            DTRSISNKSSEVGCLNLY LLELDTEATLDVLRCAFVE E LK  SSLDGPVD  M+LQ+
Sbjct: 1141 DTRSISNKSSEVGCLNLYHLLELDTEATLDVLRCAFVEVEFLKTNSSLDGPVDAIMELQD 1200

Query: 1201 EKNSISGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIA 1260
            EKNSISGRKNFLIQNVVDALVHVL KAICETDESP GDNITLVDDWPSKKELIHLFDFIA
Sbjct: 1201 EKNSISGRKNFLIQNVVDALVHVLGKAICETDESPDGDNITLVDDWPSKKELIHLFDFIA 1260

Query: 1261 TYVACGKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVI 1320
            TYVACGKATVSKDVVGQILEHLISN+ IPET SDFLPRVTANSV SRKREKQVLSLLEV+
Sbjct: 1261 TYVACGKATVSKDVVGQILEHLISNTHIPET-SDFLPRVTANSVHSRKREKQVLSLLEVV 1320

Query: 1321 PETHWNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLE 1380
            PETHWNPSSVLRMCEKAQFFQVCGLIHSI  QYSSALDSYMKDV EPIH F FINR LL+
Sbjct: 1321 PETHWNPSSVLRMCEKAQFFQVCGLIHSIGCQYSSALDSYMKDVGEPIHAFAFINRALLK 1380

Query: 1381 LGNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKT 1440
            L NSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFN+DVSNIL QLRNHPRSLFLYLKT
Sbjct: 1381 LSNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNDDVSNILLQLRNHPRSLFLYLKT 1440

Query: 1441 LIEVHLSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDIIELYV 1500
            LIEVHLSGS DFSCLKKDDNLGVNYSTKG+DDYL+KLSDFPKYLSNNPVDVTDDIIELYV
Sbjct: 1441 LIEVHLSGSLDFSCLKKDDNLGVNYSTKGLDDYLKKLSDFPKYLSNNPVDVTDDIIELYV 1500

Query: 1501 ELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSL 1560
            ELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSL
Sbjct: 1501 ELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSL 1560

Query: 1561 DKKFHDLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLN 1620
            DKKFHDLEAAVGA VSN ASSGS+DSQ+F+SVLKLQEVN V+VLLHACIGLCQRNTPRLN
Sbjct: 1561 DKKFHDLEAAVGAIVSNGASSGSSDSQHFDSVLKLQEVNTVEVLLHACIGLCQRNTPRLN 1620

Query: 1621 SEESQTLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKS 1680
             EES+TLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNE S SQKDKEANIVTWRILKS
Sbjct: 1621 CEESETLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNEPSSSQKDKEANIVTWRILKS 1680

Query: 1681 NKVAHLLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGF 1740
            NK AH+LRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGF
Sbjct: 1681 NKAAHILRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGF 1740

Query: 1741 ERRILDSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCG 1800
            ERRILD+AKALIEDDSFYTM+LLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCG
Sbjct: 1741 ERRILDTAKALIEDDSFYTMNLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCG 1800

Query: 1801 HATHLQCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVS 1860
            HATHLQCEDLENEASGGD TCPICVHSNQSQGSKSKAPTEYSLVNKFSSRT SSSGASVS
Sbjct: 1801 HATHLQCEDLENEASGGDSTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTSSSSGASVS 1860

Query: 1861 YPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLL 1920
            YPQETD+LELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLL
Sbjct: 1861 YPQETDILELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLL 1920

Query: 1921 VGESSGGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1961
            VGESS GREKVEKLNKSRQLT VKVKRPSSLRFPLK SLFGKEKMTNS
Sbjct: 1921 VGESSSGREKVEKLNKSRQLTEVKVKRPSSLRFPLKASLFGKEKMTNS 1965

BLAST of CsaV3_3G007340 vs. NCBI nr
Match: XP_038883953.1 (vacuolar protein sorting-associated protein 8 homolog isoform X1 [Benincasa hispida])

HSP 1 Score: 3437.5 bits (8912), Expect = 0.0e+00
Identity = 1765/1970 (89.59%), Postives = 1837/1970 (93.25%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSSG-GDD-DDDDDLSFPHRSIDEILNDSSSSTSP 60
            MT+ELTD ETLP MELDLNAFIHAHLSSG GDD DDDDDLSFPHRSIDEILN+SSSST  
Sbjct: 31   MTKELTDNETLPLMELDLNAFIHAHLSSGDGDDGDDDDDLSFPHRSIDEILNESSSST-- 90

Query: 61   SPSSSPHFPPPRGRRNIVAGDDGVSASPSTSPY--------KDSEAARNNPWNEKSAQLK 120
              SS PH PP RG RN  AGD GVS SPS SP+        KD E  R+N WNEKS QLK
Sbjct: 91   --SSPPHSPPSRGLRNNAAGDGGVSTSPSISPFKSPFEETIKDPETPRSNQWNEKSVQLK 150

Query: 121  PGTASHSKVGELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIK 180
            PG  SH+KVGELTDDPFRRGSRPLPSLFG VRSNAKPGAALAAAAAASRSTPAPHAAAIK
Sbjct: 151  PGAVSHAKVGELTDDPFRRGSRPLPSLFGGVRSNAKPGAALAAAAAASRSTPAPHAAAIK 210

Query: 181  SRRAGYGNMVLDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASM 240
            SRRAGYGNMVLDDDEL SSSAV+SEFFSDSLYH NIHS++SGE SIS+VDRI DYQ+ASM
Sbjct: 211  SRRAGYGNMVLDDDELVSSSAVESEFFSDSLYHTNIHSEDSGEKSISMVDRIADYQVASM 270

Query: 241  NVSGELWATNNIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLL 300
            NV GELW  +NI DGVP+NDEF +T DME EAE SSVDDV+F ES +T+P V  N+RSL 
Sbjct: 271  NVGGELWERDNIGDGVPYNDEFLITRDMECEAEPSSVDDVDFNESATTLPRVGANNRSLS 330

Query: 301  GPAEKNVCSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSK 360
            GP +KNVCS + HPT LDVDESNEGAIP   EPDDE S+VGYGSLELETQDFEK  QPSK
Sbjct: 331  GPVQKNVCSMETHPTALDVDESNEGAIPWSIEPDDEGSSVGYGSLELETQDFEKCLQPSK 390

Query: 361  DTEVDLAIEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFT 420
            DTEVDLAIEDPS V+DIIE GETTEQ DN QIG+RPE + +SSTNPL+LAEEIEKKQAFT
Sbjct: 391  DTEVDLAIEDPSEVDDIIELGETTEQLDNFQIGERPERMPLSSTNPLELAEEIEKKQAFT 450

Query: 421  ALHWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHAN 480
            ALHWEEGVAAQPMRLEGIKG TTTLGYFDIQADNSISRTISSHSF+REHGF QVLAVHAN
Sbjct: 451  ALHWEEGVAAQPMRLEGIKGGTTTLGYFDIQADNSISRTISSHSFKREHGFSQVLAVHAN 510

Query: 481  YIAVGMSKGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGY 540
            YIAVGMSKGNIVVVASK SAQNGDNMDAKM+LLGSQGDKSTAP TSLCF+QQGDLLLAGY
Sbjct: 511  YIAVGMSKGNIVVVASKSSAQNGDNMDAKMLLLGSQGDKSTAPVTSLCFNQQGDLLLAGY 570

Query: 541  SDGHITVWDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS 600
            SDGHITVWDVLRASAAKVISGEH SPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS
Sbjct: 571  SDGHITVWDVLRASAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS 630

Query: 601  VVPLLNRFSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGG 660
            VVPLLNRFS+KTQCLLDGQKTGTVLSASALLLNEF GSSLPPTLSN AVSTSSIGSMMGG
Sbjct: 631  VVPLLNRFSNKTQCLLDGQKTGTVLSASALLLNEFCGSSLPPTLSNAAVSTSSIGSMMGG 690

Query: 661  VVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYT 720
            VVGGDSGWKLFNEGSS VEEGVV+FATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYT
Sbjct: 691  VVGGDSGWKLFNEGSSFVEEGVVVFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYT 750

Query: 721  AWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDD 780
            AWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWL+D
Sbjct: 751  AWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLND 810

Query: 781  QVLVILTVTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVA 840
            QVLVILTVTGQLFLFEKDGTMIHQTSIFVDGF KEDFIAYHTHF N+LGNPEKAYHNCVA
Sbjct: 811  QVLVILTVTGQLFLFEKDGTMIHQTSIFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVA 870

Query: 841  VRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSL 900
            VRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSL
Sbjct: 871  VRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSL 930

Query: 901  ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNR 960
            ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDD+  ESH AHSEIKEQYNR
Sbjct: 931  ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDVMSESHYAHSEIKEQYNR 990

Query: 961  VGGVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020
            VGGVAVEFCVHI+RTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALV
Sbjct: 991  VGGVAVEFCVHITRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1050

Query: 1021 EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080
            EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL
Sbjct: 1051 EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1110

Query: 1081 LAVLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD 1140
            LAVLRTSKSK ASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD
Sbjct: 1111 LAVLRTSKSKRASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD 1170

Query: 1141 AVDTRSISNKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQL 1200
             VDTRSISNKSSEVG LNLY LLELDT ATLDVLRCAFVE E+LK  SS DG VD  MQL
Sbjct: 1171 PVDTRSISNKSSEVGYLNLYHLLELDTGATLDVLRCAFVEDEVLKTNSSPDGSVDARMQL 1230

Query: 1201 QEEKNSISGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDF 1260
            +EEKNSISGRKNFLIQNVVD L+HVLDKAICETD S AGDNITLV++WPSKK+LIHLFDF
Sbjct: 1231 REEKNSISGRKNFLIQNVVDGLIHVLDKAICETDGSQAGDNITLVNNWPSKKDLIHLFDF 1290

Query: 1261 IATYVACGKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLE 1320
            +ATYVACGKAT SKDVVGQILEHLIS SDI ET SD LP VTANSV SRKREKQVLSLLE
Sbjct: 1291 VATYVACGKATASKDVVGQILEHLISKSDIAETTSDCLPHVTANSVHSRKREKQVLSLLE 1350

Query: 1321 VIPETHWNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTL 1380
            V+PETHWNPSSVLRMCEKAQFFQVCGLIHSI  QYSSALD YMKD+DEPIH F FINR L
Sbjct: 1351 VVPETHWNPSSVLRMCEKAQFFQVCGLIHSIRRQYSSALDGYMKDLDEPIHAFVFINRAL 1410

Query: 1381 LELGNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYL 1440
            LEL NSE+TEFRAVVIS+IPELFNLNR ATFFLVIDHFNN+VS+ILSQL NHPRSLFLYL
Sbjct: 1411 LELSNSERTEFRAVVISQIPELFNLNREATFFLVIDHFNNEVSDILSQLHNHPRSLFLYL 1470

Query: 1441 KTLIEVHLSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDIIEL 1500
            KTLIEVHLSGS DFSCLKKD N GVNYSTKG+DDYLQKLSDFPKYLSNNPVDVTDDIIEL
Sbjct: 1471 KTLIEVHLSGSLDFSCLKKDGNFGVNYSTKGLDDYLQKLSDFPKYLSNNPVDVTDDIIEL 1530

Query: 1501 YVELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560
            YV+LLCQ+ERESVLKFLETFDSY VEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS
Sbjct: 1531 YVKLLCQYERESVLKFLETFDSYHVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1590

Query: 1561 SLDKKFHDLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPR 1620
            SLDKKFHDLEAAVG  V+N ASSGSNDSQ+F+SVLKLQEV AV+VLLHACIGLCQRNTPR
Sbjct: 1591 SLDKKFHDLEAAVGGIVTNGASSGSNDSQHFSSVLKLQEVKAVEVLLHACIGLCQRNTPR 1650

Query: 1621 LNSEESQTLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRIL 1680
            LNSEES+TLWFKLLDSFCEPLIDSYN+RTASFE+NQVQFLNESS SQK KEA+IVTWRIL
Sbjct: 1651 LNSEESETLWFKLLDSFCEPLIDSYNYRTASFEENQVQFLNESSASQKAKEAHIVTWRIL 1710

Query: 1681 KSNKVAHLLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTF 1740
            KSN+ AH+LR+LFSQFIREIVEGM GYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTF
Sbjct: 1711 KSNEAAHILRRLFSQFIREIVEGMTGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTF 1770

Query: 1741 GFERRILDSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFN 1800
            GFERRILD+AK LIEDD+FYTMSLLKKGA+HGYAP+SVVCCICNRLL+KSSSSYRVRVFN
Sbjct: 1771 GFERRILDTAKTLIEDDTFYTMSLLKKGASHGYAPQSVVCCICNRLLIKSSSSYRVRVFN 1830

Query: 1801 CGHATHLQCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGAS 1860
            CGHATHLQCED +NEASGGD+ CPICVHSNQSQ S+SKAP+EY LVNKFSSRTQSSSGAS
Sbjct: 1831 CGHATHLQCEDPDNEASGGDFICPICVHSNQSQRSRSKAPSEYGLVNKFSSRTQSSSGAS 1890

Query: 1861 VSYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYH 1920
            VSYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPP VYHDKVTKGYH
Sbjct: 1891 VSYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPTVYHDKVTKGYH 1950

Query: 1921 LLVGESSGGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1961
            LLVGESS G+EKVE+LNKSRQLT VKVKRPSSLRFPLK SLFGKEK TNS
Sbjct: 1951 LLVGESSSGKEKVERLNKSRQLTEVKVKRPSSLRFPLKASLFGKEKTTNS 1996

BLAST of CsaV3_3G007340 vs. NCBI nr
Match: XP_038883954.1 (vacuolar protein sorting-associated protein 8 homolog isoform X2 [Benincasa hispida])

HSP 1 Score: 3385.9 bits (8778), Expect = 0.0e+00
Identity = 1746/1970 (88.63%), Postives = 1816/1970 (92.18%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSSG-GDD-DDDDDLSFPHRSIDEILNDSSSSTSP 60
            MT+ELTD ETLP MELDLNAFIHAHLSSG GDD DDDDDLSFPHRSIDEILN+SSSST  
Sbjct: 31   MTKELTDNETLPLMELDLNAFIHAHLSSGDGDDGDDDDDLSFPHRSIDEILNESSSST-- 90

Query: 61   SPSSSPHFPPPRGRRNIVAGDDGVSASPSTSPY--------KDSEAARNNPWNEKSAQLK 120
              SS PH PP RG RN  AGD GVS SPS SP+        KD E  R+N WNEKS QLK
Sbjct: 91   --SSPPHSPPSRGLRNNAAGDGGVSTSPSISPFKSPFEETIKDPETPRSNQWNEKSVQLK 150

Query: 121  PGTASHSKVGELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIK 180
            PG  SH+KVGELTDDPFRRGSRPLPSLFG VRSNAKPGAALAAAAAASRSTPAPHAAAIK
Sbjct: 151  PGAVSHAKVGELTDDPFRRGSRPLPSLFGGVRSNAKPGAALAAAAAASRSTPAPHAAAIK 210

Query: 181  SRRAGYGNMVLDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASM 240
            SRRAGYGNMVLDDDEL SSSAV+SEFFSDSLYH NIHS++SGE SIS+VDRI DYQ+ASM
Sbjct: 211  SRRAGYGNMVLDDDELVSSSAVESEFFSDSLYHTNIHSEDSGEKSISMVDRIADYQVASM 270

Query: 241  NVSGELWATNNIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLL 300
            NV GELW  +NI DGVP+NDEF +T DME EAE SSVDDV+F ES +T+P V  N+RSL 
Sbjct: 271  NVGGELWERDNIGDGVPYNDEFLITRDMECEAEPSSVDDVDFNESATTLPRVGANNRSLS 330

Query: 301  GPAEKNVCSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSK 360
            GP +KNVCS + HPT LDVDESNEGAIP   EPDDE S+VGYGSLELETQDFEK  QPSK
Sbjct: 331  GPVQKNVCSMETHPTALDVDESNEGAIPWSIEPDDEGSSVGYGSLELETQDFEKCLQPSK 390

Query: 361  DTEVDLAIEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFT 420
            DTEVDLAIEDPS V+DIIE GETTEQ DN QIG+RPE + +SSTNPL+LAEEIEKKQAFT
Sbjct: 391  DTEVDLAIEDPSEVDDIIELGETTEQLDNFQIGERPERMPLSSTNPLELAEEIEKKQAFT 450

Query: 421  ALHWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHAN 480
            ALHWEEGVAAQPMRLEGIKG TTTLGYFDIQADNSISRTISSHSF+REHGF QVLAVHAN
Sbjct: 451  ALHWEEGVAAQPMRLEGIKGGTTTLGYFDIQADNSISRTISSHSFKREHGFSQVLAVHAN 510

Query: 481  YIAVGMSKGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGY 540
            YIAVGMSKGNIVVVASK SAQNGDNMDAK                      QGDLLLAGY
Sbjct: 511  YIAVGMSKGNIVVVASKSSAQNGDNMDAK----------------------QGDLLLAGY 570

Query: 541  SDGHITVWDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS 600
            SDGHITVWDVLRASAAKVISGEH SPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS
Sbjct: 571  SDGHITVWDVLRASAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS 630

Query: 601  VVPLLNRFSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGG 660
            VVPLLNRFS+KTQCLLDGQKTGTVLSASALLLNEF GSSLPPTLSN AVSTSSIGSMMGG
Sbjct: 631  VVPLLNRFSNKTQCLLDGQKTGTVLSASALLLNEFCGSSLPPTLSNAAVSTSSIGSMMGG 690

Query: 661  VVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYT 720
            VVGGDSGWKLFNEGSS VEEGVV+FATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYT
Sbjct: 691  VVGGDSGWKLFNEGSSFVEEGVVVFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYT 750

Query: 721  AWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDD 780
            AWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWL+D
Sbjct: 751  AWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLND 810

Query: 781  QVLVILTVTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVA 840
            QVLVILTVTGQLFLFEKDGTMIHQTSIFVDGF KEDFIAYHTHF N+LGNPEKAYHNCVA
Sbjct: 811  QVLVILTVTGQLFLFEKDGTMIHQTSIFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVA 870

Query: 841  VRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSL 900
            VRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSL
Sbjct: 871  VRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSL 930

Query: 901  ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNR 960
            ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDD+  ESH AHSEIKEQYNR
Sbjct: 931  ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDVMSESHYAHSEIKEQYNR 990

Query: 961  VGGVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020
            VGGVAVEFCVHI+RTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALV
Sbjct: 991  VGGVAVEFCVHITRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1050

Query: 1021 EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080
            EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL
Sbjct: 1051 EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1110

Query: 1081 LAVLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD 1140
            LAVLRTSKSK ASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD
Sbjct: 1111 LAVLRTSKSKRASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD 1170

Query: 1141 AVDTRSISNKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQL 1200
             VDTRSISNKSSEVG LNLY LLELDT ATLDVLRCAFVE E+LK  SS DG VD  MQL
Sbjct: 1171 PVDTRSISNKSSEVGYLNLYHLLELDTGATLDVLRCAFVEDEVLKTNSSPDGSVDARMQL 1230

Query: 1201 QEEKNSISGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDF 1260
            +EEKNSISGRKNFLIQNVVD L+HVLDKAICETD S AGDNITLV++WPSKK+LIHLFDF
Sbjct: 1231 REEKNSISGRKNFLIQNVVDGLIHVLDKAICETDGSQAGDNITLVNNWPSKKDLIHLFDF 1290

Query: 1261 IATYVACGKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLE 1320
            +ATYVACGKAT SKDVVGQILEHLIS SDI ET SD LP VTANSV SRKREKQVLSLLE
Sbjct: 1291 VATYVACGKATASKDVVGQILEHLISKSDIAETTSDCLPHVTANSVHSRKREKQVLSLLE 1350

Query: 1321 VIPETHWNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTL 1380
            V+PETHWNPSSVLRMCEKAQFFQVCGLIHSI  QYSSALD YMKD+DEPIH F FINR L
Sbjct: 1351 VVPETHWNPSSVLRMCEKAQFFQVCGLIHSIRRQYSSALDGYMKDLDEPIHAFVFINRAL 1410

Query: 1381 LELGNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYL 1440
            LEL NSE+TEFRAVVIS+IPELFNLNR ATFFLVIDHFNN+VS+ILSQL NHPRSLFLYL
Sbjct: 1411 LELSNSERTEFRAVVISQIPELFNLNREATFFLVIDHFNNEVSDILSQLHNHPRSLFLYL 1470

Query: 1441 KTLIEVHLSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDIIEL 1500
            KTLIEVHLSGS DFSCLKKD N GVNYSTKG+DDYLQKLSDFPKYLSNNPVDVTDDIIEL
Sbjct: 1471 KTLIEVHLSGSLDFSCLKKDGNFGVNYSTKGLDDYLQKLSDFPKYLSNNPVDVTDDIIEL 1530

Query: 1501 YVELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560
            YV+LLCQ+ERESVLKFLETFDSY VEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS
Sbjct: 1531 YVKLLCQYERESVLKFLETFDSYHVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1590

Query: 1561 SLDKKFHDLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPR 1620
            SLDKKFHDLEAAVG  V+N ASSGSNDSQ+F+SVLKLQEV AV+VLLHACIGLCQRNTPR
Sbjct: 1591 SLDKKFHDLEAAVGGIVTNGASSGSNDSQHFSSVLKLQEVKAVEVLLHACIGLCQRNTPR 1650

Query: 1621 LNSEESQTLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRIL 1680
            LNSEES+TLWFKLLDSFCEPLIDSYN+RTASFE+NQVQFLNESS SQK KEA+IVTWRIL
Sbjct: 1651 LNSEESETLWFKLLDSFCEPLIDSYNYRTASFEENQVQFLNESSASQKAKEAHIVTWRIL 1710

Query: 1681 KSNKVAHLLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTF 1740
            KSN+ AH+LR+LFSQFIREIVEGM GYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTF
Sbjct: 1711 KSNEAAHILRRLFSQFIREIVEGMTGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTF 1770

Query: 1741 GFERRILDSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFN 1800
            GFERRILD+AK LIEDD+FYTMSLLKKGA+HGYAP+SVVCCICNRLL+KSSSSYRVRVFN
Sbjct: 1771 GFERRILDTAKTLIEDDTFYTMSLLKKGASHGYAPQSVVCCICNRLLIKSSSSYRVRVFN 1830

Query: 1801 CGHATHLQCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGAS 1860
            CGHATHLQCED +NEASGGD+ CPICVHSNQSQ S+SKAP+EY LVNKFSSRTQSSSGAS
Sbjct: 1831 CGHATHLQCEDPDNEASGGDFICPICVHSNQSQRSRSKAPSEYGLVNKFSSRTQSSSGAS 1890

Query: 1861 VSYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYH 1920
            VSYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPP VYHDKVTKGYH
Sbjct: 1891 VSYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPTVYHDKVTKGYH 1950

Query: 1921 LLVGESSGGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1961
            LLVGESS G+EKVE+LNKSRQLT VKVKRPSSLRFPLK SLFGKEK TNS
Sbjct: 1951 LLVGESSSGKEKVERLNKSRQLTEVKVKRPSSLRFPLKASLFGKEKTTNS 1974

BLAST of CsaV3_3G007340 vs. NCBI nr
Match: KAG6582725.1 (Vacuolar protein sorting-associated protein 8-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 3307.7 bits (8575), Expect = 0.0e+00
Identity = 1707/1970 (86.65%), Postives = 1803/1970 (91.52%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSSGGDDDDD--DDLSFPHRSIDEILNDSSSSTSP 60
            MT+ELTDTETLPPMELDLNAFIHAHLSSG DDDDD  DDLSFPHRSIDEILN+SSSSTS 
Sbjct: 1    MTKELTDTETLPPMELDLNAFIHAHLSSGDDDDDDDEDDLSFPHRSIDEILNESSSSTSS 60

Query: 61   SPSSSPHFPPPRGRRNIVAGDDGVSASPSTSPYKD--------SEAARNNPWNEKSAQLK 120
            SPSS P+ PPPR RR+I A D   SAS S  P+K         S+  R+N  NEKS QLK
Sbjct: 61   SPSSPPNSPPPRDRRSIAARDGRASASRSIPPFKSPFEEIIKASKVPRSNQRNEKSVQLK 120

Query: 121  PGTASHSKVGELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIK 180
            PG+ASH+KVGELTDDPFRRGSR LPSLFG VRSNAKPGAALAAAAAASRS PAPHAAAIK
Sbjct: 121  PGSASHTKVGELTDDPFRRGSRALPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIK 180

Query: 181  SRRAGYGNMVLDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASM 240
            SRR+G+G++VLDDDELASSSAVDSEF  D LY    HSKES E SIS+V+R  DYQ AS+
Sbjct: 181  SRRSGHGSVVLDDDELASSSAVDSEFVFDDLYPTIDHSKESREKSISLVERNADYQGASV 240

Query: 241  NVSGELWATNNIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLL 300
            NV GE WA +NIRD V +NDEFR+T+D E EAE S VDDVNF ES +T+PPVE N RSL 
Sbjct: 241  NVGGEFWARDNIRDCVLYNDEFRITKDTECEAEQSFVDDVNFDESSTTLPPVEANGRSLS 300

Query: 301  GPAEKNVCSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSK 360
              A+ NVCS DA PT LDVDESNEGA P   +PD + SAVGYG LELETQDFEK  QPSK
Sbjct: 301  DSADNNVCSMDAEPTVLDVDESNEGAFPCSPKPDYDRSAVGYGKLELETQDFEKRFQPSK 360

Query: 361  DTEVDLAIEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFT 420
            D+EV LAIED S+VNDI ES ET EQ DN   G+R E +S+SS+NPL+LAEEIEKKQAFT
Sbjct: 361  DSEV-LAIEDLSVVNDISESRETAEQLDNFHTGERAETMSLSSSNPLELAEEIEKKQAFT 420

Query: 421  ALHWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHAN 480
            ALHWEEGVAAQPMRLEGIKG TT LGYFDIQADNSISRTISSHSF+REHGFPQ LAVHAN
Sbjct: 421  ALHWEEGVAAQPMRLEGIKGGTTALGYFDIQADNSISRTISSHSFKREHGFPQALAVHAN 480

Query: 481  YIAVGMSKGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGY 540
            YIAVGMSKGNIVVVASKYSAQNGDNMDAKM+LLGSQGDKS AP TSLCF+QQGDLLLAGY
Sbjct: 481  YIAVGMSKGNIVVVASKYSAQNGDNMDAKMLLLGSQGDKSNAPVTSLCFNQQGDLLLAGY 540

Query: 541  SDGHITVWDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS 600
            SDG +TVWDVLRA+AAKVISGEH SPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS
Sbjct: 541  SDGQVTVWDVLRATAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS 600

Query: 601  VVPLLNRFSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGG 660
            VVPLLNRFS KTQCLLDGQKTGTVLSASALLLNEF GSSLPP+LSNVAVSTSSIGSMMGG
Sbjct: 601  VVPLLNRFSIKTQCLLDGQKTGTVLSASALLLNEFCGSSLPPSLSNVAVSTSSIGSMMGG 660

Query: 661  VVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYT 720
            VVGGDSGWKLFNEGSSLV EGVVIFATHQTALVVRLSPTVEVYA+LSKPDGI+EGSMPYT
Sbjct: 661  VVGGDSGWKLFNEGSSLV-EGVVIFATHQTALVVRLSPTVEVYARLSKPDGIQEGSMPYT 720

Query: 721  AWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDD 780
            AWKCSQS ETSPSEAVER+SLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGV WLDD
Sbjct: 721  AWKCSQSIETSPSEAVERISLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVAWLDD 780

Query: 781  QVLVILTVTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVA 840
            QVLVILTVTGQLFLFEKDGTMIHQTS+FVDGF KEDFIA+HTHF N+LGNPEKAYHNCVA
Sbjct: 781  QVLVILTVTGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAHHTHFVNVLGNPEKAYHNCVA 840

Query: 841  VRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSL 900
            VRGAS+YVLGP HLVISRLLPWKERVQVLRKAGDWM+ALSMAITIYDGHAHGVIDLPRSL
Sbjct: 841  VRGASVYVLGPKHLVISRLLPWKERVQVLRKAGDWMTALSMAITIYDGHAHGVIDLPRSL 900

Query: 901  ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNR 960
            ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDD+T  SHSAHSEIKEQYNR
Sbjct: 901  ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDVTSGSHSAHSEIKEQYNR 960

Query: 961  VGGVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020
            VGGVAVEFCVHI+RTDILFDEIFSKFV VQQRDTFLELLEPYILKDMLGSLPPEIMQALV
Sbjct: 961  VGGVAVEFCVHITRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020

Query: 1021 EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080
            EHYS KGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL
Sbjct: 1021 EHYSQKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080

Query: 1081 LAVLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD 1140
            LAVL+TSKSK AS++GYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD
Sbjct: 1081 LAVLQTSKSKRASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD 1140

Query: 1141 AVDTRSISNKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQL 1200
             VDT+SISNKSSEVG LNLY LLELDT ATLDVLRCAFVEGEI+KA SSLDG VD SMQ+
Sbjct: 1141 TVDTKSISNKSSEVGYLNLYHLLELDTGATLDVLRCAFVEGEIVKADSSLDGSVDASMQV 1200

Query: 1201 QEEKNSISGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDF 1260
            Q+EKNS SGRKNFL+QNVVDAL+H+LDKAI +T  SP GDNITLV+DWPSKK+L HLFDF
Sbjct: 1201 QKEKNSTSGRKNFLVQNVVDALIHILDKAISQTYGSPGGDNITLVEDWPSKKDLFHLFDF 1260

Query: 1261 IATYVACGKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLE 1320
            +A YVACGKAT SKDVVGQILEHLISNSDIPE   D +  VTANSV SRKREKQVLSLLE
Sbjct: 1261 VANYVACGKATASKDVVGQILEHLISNSDIPEMEIDSVHSVTANSVHSRKREKQVLSLLE 1320

Query: 1321 VIPETHWNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTL 1380
            VIPETHWNPSSVLRMCEKAQFFQVCGLIHSI+HQYSSALD YMKDV+EPIH F FINRTL
Sbjct: 1321 VIPETHWNPSSVLRMCEKAQFFQVCGLIHSISHQYSSALDGYMKDVEEPIHAFAFINRTL 1380

Query: 1381 LELGNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYL 1440
            +EL  SEQTEFR VVISRIPEL NLNR  TFFLVIDHF NDVS+ILSQL NHPRSLFLYL
Sbjct: 1381 MELSYSEQTEFRGVVISRIPELLNLNREGTFFLVIDHFGNDVSDILSQLHNHPRSLFLYL 1440

Query: 1441 KTLIEVHLSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDIIEL 1500
            KTLIEVH SG+ +FSCLKKDDN GVNYSTKG+DDYLQKLSDFPK+LSNNPVDVTDDIIEL
Sbjct: 1441 KTLIEVHQSGNLNFSCLKKDDNFGVNYSTKGLDDYLQKLSDFPKFLSNNPVDVTDDIIEL 1500

Query: 1501 YVELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560
            YVELLCQ+ERESVLKFLETFDSY VEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS
Sbjct: 1501 YVELLCQYERESVLKFLETFDSYHVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560

Query: 1561 SLDKKFHDLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPR 1620
            SLDKKFHDLEAAVG  V+N ASSGSNDSQ F+SVLKLQEVN + VLLHACIGLCQRNTPR
Sbjct: 1561 SLDKKFHDLEAAVGGIVTNGASSGSNDSQLFSSVLKLQEVNDIYVLLHACIGLCQRNTPR 1620

Query: 1621 LNSEESQTLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRIL 1680
            LNSEES+TLWFKLLDSFCEPLI+S+N+RTASF +NQVQFLNESS SQKDKEA+IVTWRIL
Sbjct: 1621 LNSEESETLWFKLLDSFCEPLIESFNYRTASFGENQVQFLNESSGSQKDKEAHIVTWRIL 1680

Query: 1681 KSNKVAHLLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTF 1740
            KSN+ AH+LR LFS+FIREIVEGM+GYVHLPTIMSRLL DNGSQEFGDFKLTILGMLGTF
Sbjct: 1681 KSNQTAHILRSLFSRFIREIVEGMIGYVHLPTIMSRLLSDNGSQEFGDFKLTILGMLGTF 1740

Query: 1741 GFERRILDSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFN 1800
            GFERRILD+AKALIEDD+FYTMSLLKKGA+HGYAPR  VCCICNRLLVKSSSSYRVRVFN
Sbjct: 1741 GFERRILDTAKALIEDDTFYTMSLLKKGASHGYAPRGAVCCICNRLLVKSSSSYRVRVFN 1800

Query: 1801 CGHATHLQCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGAS 1860
            CGHATHLQCE L+NEASG D++CP+CVHSN SQ S+ KA TEYSLVNKFSSRTQSSSGAS
Sbjct: 1801 CGHATHLQCEVLDNEASGADFSCPVCVHSNHSQRSRGKALTEYSLVNKFSSRTQSSSGAS 1860

Query: 1861 VSYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYH 1920
            VSYPQETDLLELPYTLQQIPRFEIL NLQKNQRVIDIEN+PQLRLAPPAVYHDKVTKGYH
Sbjct: 1861 VSYPQETDLLELPYTLQQIPRFEILANLQKNQRVIDIENMPQLRLAPPAVYHDKVTKGYH 1920

Query: 1921 LLVGESSGGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1961
            LLVG+SSGG EKVEKLNKSRQL  VKVKRPSSLRFPLK +LFGKEK T S
Sbjct: 1921 LLVGDSSGGIEKVEKLNKSRQLKEVKVKRPSSLRFPLKANLFGKEKTTKS 1968

BLAST of CsaV3_3G007340 vs. ExPASy Swiss-Prot
Match: Q0P5W1 (Vacuolar protein sorting-associated protein 8 homolog OS=Mus musculus OX=10090 GN=Vps8 PE=1 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 2.8e-91
Identity = 347/1443 (24.05%), Postives = 612/1443 (42.41%), Query Frame = 0

Query: 446  ISRTISSHSFRREHGFPQVLAVHANYIAVGMSKGNIVVVASKYSAQNGDNMDAKMILLGS 505
            IS  I S + + + G P  +AV ++ IAVG S G  ++          D   A  + LGS
Sbjct: 141  ISAQIVSAADKVDAGLPTAIAV-SSLIAVGTSHGLALIF---------DQNQALRLCLGS 200

Query: 506  QGDKSTAPATS-LCFSQQGDLLLAGYSDGHITVWDVLRASAAKVISGEH--ASPVVHSLF 565
                    A S L  +     LL G++ G IT+WD+      + I+  H   + ++H  F
Sbjct: 201  TSVGGQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSITDAHPPGTAILHIKF 260

Query: 566  LGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSSKTQCLLDGQKTGTVLSASALLL 625
                        A+  DS G V   TF  V  +   + +++CL  G K G V     L  
Sbjct: 261  TDDPT------LAICNDSGGSVFELTFKRV--MGVRTCESRCLFSGSK-GEVCCIEPL-- 320

Query: 626  NEFVGSSLPPTLSNVAVSTSSIGSMMGGVVGGDSGWKLFNEGSSLVEEGVVIFATHQTAL 685
                     P L +  ++  S+ +M                            A+    L
Sbjct: 321  ------HSKPELKDHPITQFSLLAM----------------------------ASLTKIL 380

Query: 686  VVRLSPTVEVYAQLSKPDG-IREGSMPYTAWK-CSQSFETSPSEAVERVSLLAIAWDKMV 745
            V+ L P+++V+  ++ P G +   S+P  AW   + +   +P  A  R  ++     K  
Sbjct: 381  VIGLKPSLKVW--MTFPYGRMDPSSVPLLAWHFVAVNNSVNPMLAFCRGDMVHFLLVKRD 440

Query: 746  QVAKLVKTELKVCGKWSLESAAIGVVWLDDQVLVILTVTGQLFLFEKDGTMIHQTSIFVD 805
            +   +  T+ K      L    I   W++ + +V+L    +L + ++      Q  +   
Sbjct: 441  ESGAIHVTKQK---HLHLYYDLINFTWINSRTVVLLDSVEKLHVIDRQ----TQEELETM 500

Query: 806  GFVKEDFIAYHTHFANIL--GN--------PEKAYHNCVAVRGASIYVLGPMHLVISRLL 865
               +   +   +HF ++   GN         EKA +  ++  G  I+ LG   + +  L 
Sbjct: 501  EISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLR 560

Query: 866  PWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQELVMPFLIELLLSYVD 925
             W+ER+  L K      AL++A + ++G A  V+ L   +   + +V   ++E+L  Y D
Sbjct: 561  SWRERMDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDVSKRKAVVADRMVEILFHYAD 620

Query: 926  EVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVGGVAVEFCVHISRTDILFD 985
                             +K  D           +++ +     V V++C+ + R D+LF 
Sbjct: 621  RAL--------------KKCPD-----QGKIQVMEQHFQDTVPVIVDYCLLLQRKDLLFG 680

Query: 986  EIFSKF-VGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQRVEQCVLHM 1045
            +++ K       +  FLE LEPYIL D L  + P++M+ L+ H+  K  L+ VE  ++HM
Sbjct: 681  QMYDKLSENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLLENVEALIVHM 740

Query: 1046 DISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLR------TSKSKHAS 1105
            DI+SLD  QVV +C ++ LY A+VY++N+G+++F +P+E+L  V+        + +    
Sbjct: 741  DITSLDIQQVVLMCWENRLYDAMVYVYNRGMNEFISPMEKLFKVIAPPLNAGKTLTDEQV 800

Query: 1106 SLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVDTRSISNKSSE 1165
             +G K LVY+  C +G A+P   G +    V  +++++ +FL+         S+   S E
Sbjct: 801  VMGNKLLVYISCCLAGRAYP--LGDIPEDLVPLVKNQVFEFLIR------LHSVEASSEE 860

Query: 1166 VGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQEEKNSISGRKNF 1225
                 +  LL  DT   L+VL   F +                    + +K ++  +   
Sbjct: 861  EVYPYVRTLLHFDTREFLNVLALTFED-------------------FKNDKQAVEYQ--- 920

Query: 1226 LIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIATYVACGKAT-- 1285
              Q +VD L+ V+   +  +D +P+              ++  LF F+A  +A    T  
Sbjct: 921  --QRIVDILLKVM---VENSDFTPS--------------QVGCLFTFLARQLAKPDNTLF 980

Query: 1286 VSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVIPETHWNPSS 1345
            V++ +  Q+LE L S  D                    +R++ +L LL+      +  S 
Sbjct: 981  VNRTLFDQVLEFLCSPDD---------------DSRHSERQQVLLELLQAGGIVQFEESR 1040

Query: 1346 VLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLELGNS--EQT 1405
            ++RM EKA+F+Q+C  ++   HQY   +D Y+ D       F +I+  L   G+S  E+ 
Sbjct: 1041 LIRMAEKAEFYQICEFMYEREHQYDKIIDCYLHDPLREEEVFNYIHNILSIPGHSAEEKQ 1100

Query: 1406 EFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKTLIEVHLS 1465
                  ++ + EL +L       LV  HF+  +  ++ QL+N    LF +L++L+     
Sbjct: 1101 SVWQKAMNHMEELVSLKPCKAAELVATHFSEQIEVVIGQLQNQ-LLLFKFLRSLL----- 1160

Query: 1466 GSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDIIELYVELLCQHE 1525
                      D   GV+ +        Q+L   P +           I E ++ELLCQ  
Sbjct: 1161 ----------DPREGVHVN--------QELLQIPPH-----------ITEQFIELLCQFS 1220

Query: 1526 RESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFHDL 1585
             + V++ L+  + YR+E  +++ Q+Y++ +  A+LLE+ GD   A  L L  L  +  ++
Sbjct: 1221 PDQVIQTLQVLECYRLEETIQITQKYQLHEVTAYLLEKKGDAHGAFLLLLERLQSRLQEM 1280

Query: 1586 EAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLNSEESQTL 1645
                           +   +N    + L+ V    V     I LCQRN+  LN ++ + L
Sbjct: 1281 ---------------TRQDENTKEDILLKGVEDTMV---ETIALCQRNSQNLNQQQREAL 1340

Query: 1646 WFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKSNKVAHLL 1705
            WF LL++   P                 Q L+ S+ +                    H  
Sbjct: 1341 WFPLLEAMMTP-----------------QKLSSSAAA-------------------PHPH 1342

Query: 1706 RKLFSQFIREIVEGMMGYVHLPTIMSRLLYD--NGSQEFGDFKLTILGMLGTFGFERRIL 1765
             +       +++  M  ++ LP+I+ R+L D   G  + G+ +  ILGML TF +E+ +L
Sbjct: 1401 CEALKSLTMQVLNSMAAFIALPSILQRILQDPIYGKGKLGEIQGLILGMLDTFNYEQTLL 1342

Query: 1766 DSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCIC-NRLLVKSSSSYRVRVFNCGHATH 1825
            ++  +L+  D  +++  L+   + G  P+   C IC  +   +   +  + VF+CGH  H
Sbjct: 1461 ETTASLLNQDLHWSLCNLRASVSRGLNPKQDYCSICLQQYKRRQEMADEIIVFSCGHLYH 1342

Query: 1826 ---LQCEDLENEASGGD-YTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVS 1856
               LQ ++   E  G   + C  C  SN++ G  S+ P+E    NK    T S    S S
Sbjct: 1521 SFCLQSKECTLEVEGQTRWACHKCSSSNKA-GKLSENPSE----NKKGRITSSQVKMSPS 1342

BLAST of CsaV3_3G007340 vs. ExPASy Swiss-Prot
Match: Q8N3P4 (Vacuolar protein sorting-associated protein 8 homolog OS=Homo sapiens OX=9606 GN=VPS8 PE=1 SV=3)

HSP 1 Score: 337.8 bits (865), Expect = 8.2e-91
Identity = 336/1414 (23.76%), Postives = 600/1414 (42.43%), Query Frame = 0

Query: 446  ISRTISSHSFRREHGFPQVLAVHANYIAVGMSKGNIVVVASKYSAQNGDNMDAKMILLGS 505
            IS  I S + + + G P  +AV ++ IAVG S G  ++          D   A  + LGS
Sbjct: 141  ISAQIVSAADKVDAGLPTAIAV-SSLIAVGTSHGLALIFGK-------DQNQALRLCLGS 200

Query: 506  QGDKSTAPATS-LCFSQQGDLLLAGYSDGHITVWDVLRASAAKVISGEH--ASPVVHSLF 565
                    A S L  +     LL G++ G IT+WD+      + I+  H   + ++H  F
Sbjct: 201  TSVGGQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSITDAHPPGTAILHIKF 260

Query: 566  LGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSSKTQCLLDGQKTGTVLSASALLL 625
                        A+  DS G V   TF  V  +   + +++CL  G K G V     L  
Sbjct: 261  TDDPT------LAICNDSGGSVFELTFKRV--MGVRTCESRCLFSGSK-GEVCCIEPL-- 320

Query: 626  NEFVGSSLPPTLSNVAVSTSSIGSMMGGVVGGDSGWKLFNEGSSLVEEGVVIFATHQTAL 685
                     P L +  ++  S+ +M                            A+    L
Sbjct: 321  ------HSKPELKDHPITQFSLLAM----------------------------ASLTKIL 380

Query: 686  VVRLSPTVEVYAQLSKPDG-IREGSMPYTAWK--CSQSFETSPSEAVERVSLLAIAWDKM 745
            V+ L P+++V+  ++ P G +   S+P  AW     Q++  +P  A  R  ++     K 
Sbjct: 381  VIGLKPSLKVW--MTFPYGRMDPSSVPLLAWHFVAVQNY-VNPMLAFCRGDVVHFLLVKR 440

Query: 746  VQVAKLVKTELKVCGKWSLESAAIGVVWLDDQVLVILTVTGQLFLFEKDGTMIHQTSIFV 805
             +   +  T+ K      L    I   W++ + +V+L    +L + ++      Q  +  
Sbjct: 441  DESGAIHVTKQK---HLHLYYDLINFTWINSRTVVLLDSVEKLHVIDRQ----TQEELET 500

Query: 806  DGFVKEDFIAYHTHFANIL--GN--------PEKAYHNCVAVRGASIYVLGPMHLVISRL 865
                +   +   +HF ++   GN         EKA +  ++  G  I+ LG   + +  L
Sbjct: 501  VEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMML 560

Query: 866  LPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQELVMPFLIELLLSYV 925
              W+ERV  L K      AL++A + ++G A  V+ L       + +V   ++E+L  Y 
Sbjct: 561  RSWRERVDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDASKRKAIVADRMVEILFHYA 620

Query: 926  DEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVGGVAVEFCVHISRTDILF 985
            D                 +K  D           +++ +  +  V V++C+ + R D+LF
Sbjct: 621  DRAL--------------KKCPD-----QGKIQVMEQHFQDMVPVIVDYCLLLQRKDLLF 680

Query: 986  DEIFSKF-VGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQRVEQCVLH 1045
             +++ K       +  FLE LEPYIL D L  + P++M+ L+ H+  K  ++ VE  ++H
Sbjct: 681  SQMYDKLSENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEALIVH 740

Query: 1046 MDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLR------TSKSKHA 1105
            MDI+SLD  QVV +C ++ LY A++Y++N+G+++F +P+E+L  V+        + +   
Sbjct: 741  MDITSLDIQQVVLMCWENRLYDAMIYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLTDEQ 800

Query: 1106 SSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVDTRSISNKSS 1165
              +G K LVY+  C +G A+P   G +    V  +++++ +FL+    A         S 
Sbjct: 801  VVMGNKLLVYISCCLAGRAYP--LGDIPEDLVPLVKNQVFEFLIRLHSA-------EASP 860

Query: 1166 EVGCLNLYP----LLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQEEKNSIS 1225
            E     +YP    LL  DT   L+VL   F +                    + +K ++ 
Sbjct: 861  EE---EIYPYIRTLLHFDTREFLNVLALTFED-------------------FKNDKQAVE 920

Query: 1226 GRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIATYVACG 1285
             +     Q +VD L+ V+   +  +D +P+              ++  LF F+A  +A  
Sbjct: 921  YQ-----QRIVDILLKVM---VENSDFTPS--------------QVGCLFTFLARQLAKP 980

Query: 1286 KAT--VSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVIPETH 1345
              T  V++ +  Q+LE L S  D                    +R++ +L LL+      
Sbjct: 981  DNTLFVNRTLFDQVLEFLCSPDD---------------DSRHSERQQVLLELLQAGGIVQ 1040

Query: 1346 WNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLELGNS 1405
            +  S ++RM EKA+F+Q+C  ++   HQY   +D Y++D       F +I+  L   G+S
Sbjct: 1041 FEESRLIRMAEKAEFYQICEFMYEREHQYDKIIDCYLRDPLREEEVFNYIHNILSIPGHS 1100

Query: 1406 --EQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKTLI 1465
              E+       +  I EL +L       LV  HF+  +  ++ +L+N    LF +L++L+
Sbjct: 1101 AEEKQSVWQKAMDHIEELVSLKPCKAAELVATHFSGHIETVIKKLQNQV-LLFKFLRSLL 1160

Query: 1466 EVHLSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDIIELYVEL 1525
                           D   G+                   +++   + ++  I E ++EL
Sbjct: 1161 ---------------DPREGI-------------------HVNQELLQISPCITEQFIEL 1220

Query: 1526 LCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDK 1585
            LCQ     V++ L+  + YR+E  +++ Q+Y++ +  A+LLE+ GD+  A  + L  L  
Sbjct: 1221 LCQFNPTQVIETLQVLECYRLEETIQITQKYQLHEVTAYLLEKKGDIHGAFLIMLERLQS 1280

Query: 1586 KFHDLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLNSE 1645
            K  ++               ++  +N      L++V    V     I LCQRN+  LN +
Sbjct: 1281 KLQEV---------------THQGENTKEDPSLKDVEDTMV---ETIALCQRNSHNLNQQ 1314

Query: 1646 ESQTLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKSNK 1705
            + + LWF LL++   P                          QK           L S+ 
Sbjct: 1341 QREALWFPLLEAMMAP--------------------------QK-----------LSSSA 1314

Query: 1706 VAHLLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYD--NGSQEFGDFKLTILGMLGTFGF 1765
            + HL  +       +++  M  ++ LP+I+ R+L D   G  + G+ +  ILGML TF +
Sbjct: 1401 IPHLHSEALKSLTMQVLNSMAAFIALPSILQRILQDPVYGKGKLGEIQGLILGMLDTFNY 1314

Query: 1766 ERRILDSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCIC-NRLLVKSSSSYRVRVFNC 1822
            E+ +L++  +L+  D  +++  L+     G  P+   C IC  +   +   +  + VF+C
Sbjct: 1461 EQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCSICLQQYKRRQEMADEIIVFSC 1314

BLAST of CsaV3_3G007340 vs. ExPASy Swiss-Prot
Match: Q9VRX2 (Vacuolar protein sorting-associated protein 8 homolog OS=Drosophila melanogaster OX=7227 GN=Vps8 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 9.5e-31
Identity = 148/615 (24.07%), Postives = 257/615 (41.79%), Query Frame = 0

Query: 824  AYHNCVAVRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGV 883
            A +N V  RG  +YVLG   L I  +  W ER+  L K   W  A  +A+   DG+   V
Sbjct: 435  ACYNSVVSRGTQLYVLGARSLHIIGVRTWSERISFLVKHHRWQEACQLAL---DGYIASV 494

Query: 884  IDLPRSLESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSE 943
             D PR     +E ++                                  M  + + A+S 
Sbjct: 495  -DRPRKRAQAKERII----------------------------------MLFKEYIANSA 554

Query: 944  IKEQYNRVGGVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPP 1003
               +Y    G  V   + +   D+L+ +++ K       + FL+ +  +I K+ + S+ P
Sbjct: 555  RAPEY--CLGAIVNCLITVGELDLLWTQLWEKLHN-SSTELFLQHISEHIEKETIHSVNP 614

Query: 1004 EIMQALVEHYSHKGWLQ----RVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKG 1063
             I QALV++     WL+    ++EQ +L +D   LD NQV++  + H L+ A +YL  + 
Sbjct: 615  VISQALVDY-----WLEHSPAKLEQLILKLDWMCLDLNQVLKAVKKHRLFRAQIYLNTQA 674

Query: 1064 LDDFRTPLEELLAVLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRD 1123
            L+D+   L ELL ++   +    + LG   LVY+  C +G  +P G+  +    V  ++ 
Sbjct: 675  LNDYTAALTELLPLVTPDE----TDLGNCLLVYVSSCLAGREYPSGE--IPVELVHQVKH 734

Query: 1124 ELLQFLLENSDAVDTRSISNKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISS 1183
            ++L+ L          S  N   E+    L  LL+ DT  TL+V+  AF E E    +  
Sbjct: 735  DVLRCL------TSQHSKENAGDELPYPYLRALLKFDTRETLNVISLAFQEREFSNEL-- 794

Query: 1184 LDGPVDTSMQLQEEKNSISGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWP 1243
                             IS RK      +++ L+ ++         SP  +N T    W 
Sbjct: 795  ----------------GISHRK-----RIINLLLEIM---------SP--ENAT----W- 854

Query: 1244 SKKELIHLFDFIATYVACGKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTA-NSVLS 1303
               E+  L +FIA  ++       + ++ ++L HL       E+      R  A + +LS
Sbjct: 855  --AEIGCLLNFIAQQISMQCLPRDRQLLERVLSHLAQEEIANESSRQHSERENAWHELLS 914

Query: 1304 RKREKQVLSLLEVIPETHWNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDE 1363
                 ++ S               LR+ EKA+ + V   +     +Y + LDSY+++   
Sbjct: 915  SNCLAEISS-----------DEEQLRLAEKAKCYCVVEYLLEKLERYDTILDSYIRNEAR 935

Query: 1364 PIHTFTFINRTLLELGNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQ 1423
                F ++ R    + + +++ FR +    + EL  +N   T  L+  H+   ++ +L  
Sbjct: 975  HETMFAYMER---HVASPKRSIFRQLK-RNLRELLTINAKETTRLLSLHYPEKINELLDN 935

Query: 1424 LRNHPRSLFLYLKTL 1434
            LR     L+L+LK L
Sbjct: 1035 LRREENLLYLFLKCL 935

BLAST of CsaV3_3G007340 vs. ExPASy Swiss-Prot
Match: P39702 (Vacuolar protein sorting-associated protein 8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=VPS8 PE=1 SV=2)

HSP 1 Score: 67.4 bits (163), Expect = 2.1e-09
Identity = 31/157 (19.75%), Postives = 83/157 (52.87%), Query Frame = 0

Query: 975  KFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQRVEQCVLHMDISSL 1034
            +F  ++    + E++   + +  + S+ P + ++++++Y+ +  L+ +E  ++ ++ ++L
Sbjct: 529  EFFELKDNAVYFEVVANIVAQGSVTSISPVLFRSIIDYYAKEENLKVIEDLIIMLNPTTL 588

Query: 1035 DFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLRTSKSK----HASSLGYKTL 1094
            D +  V+LC+ + L+  L+Y++NK  DD++TP+ +L+  +     K    +   +  +T 
Sbjct: 589  DVDLAVKLCQKYNLFDLLIYIWNKIFDDYQTPVVDLIYRISNQSEKCVIFNGPQVPPETT 648

Query: 1095 V--YLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFL 1126
            +  Y+ Y  +G  +P         +   ++ EL  F+
Sbjct: 649  IFDYVTYILTGRQYPQNLSISPSDKCSKIQRELSAFI 685

BLAST of CsaV3_3G007340 vs. ExPASy TrEMBL
Match: A0A0A0L2X7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G116870 PE=3 SV=1)

HSP 1 Score: 3843.1 bits (9965), Expect = 0.0e+00
Identity = 1960/1960 (100.00%), Postives = 1960/1960 (100.00%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSSGGDDDDDDDLSFPHRSIDEILNDSSSSTSPSP 60
            MTEELTDTETLPPMELDLNAFIHAHLSSGGDDDDDDDLSFPHRSIDEILNDSSSSTSPSP
Sbjct: 1    MTEELTDTETLPPMELDLNAFIHAHLSSGGDDDDDDDLSFPHRSIDEILNDSSSSTSPSP 60

Query: 61   SSSPHFPPPRGRRNIVAGDDGVSASPSTSPYKDSEAARNNPWNEKSAQLKPGTASHSKVG 120
            SSSPHFPPPRGRRNIVAGDDGVSASPSTSPYKDSEAARNNPWNEKSAQLKPGTASHSKVG
Sbjct: 61   SSSPHFPPPRGRRNIVAGDDGVSASPSTSPYKDSEAARNNPWNEKSAQLKPGTASHSKVG 120

Query: 121  ELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRRAGYGNMV 180
            ELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRRAGYGNMV
Sbjct: 121  ELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRRAGYGNMV 180

Query: 181  LDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNVSGELWATN 240
            LDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNVSGELWATN
Sbjct: 181  LDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNVSGELWATN 240

Query: 241  NIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGPAEKNVCST 300
            NIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGPAEKNVCST
Sbjct: 241  NIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGPAEKNVCST 300

Query: 301  DAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSKDTEVDLAIED 360
            DAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSKDTEVDLAIED
Sbjct: 301  DAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSKDTEVDLAIED 360

Query: 361  PSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFTALHWEEGVAA 420
            PSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFTALHWEEGVAA
Sbjct: 361  PSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFTALHWEEGVAA 420

Query: 421  QPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYIAVGMSKGN 480
            QPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYIAVGMSKGN
Sbjct: 421  QPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYIAVGMSKGN 480

Query: 481  IVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGYSDGHITVWDV 540
            IVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGYSDGHITVWDV
Sbjct: 481  IVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGYSDGHITVWDV 540

Query: 541  LRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSS 600
            LRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSS
Sbjct: 541  LRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSS 600

Query: 601  KTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVVGGDSGWKL 660
            KTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVVGGDSGWKL
Sbjct: 601  KTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVVGGDSGWKL 660

Query: 661  FNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYTAWKCSQSFET 720
            FNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYTAWKCSQSFET
Sbjct: 661  FNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYTAWKCSQSFET 720

Query: 721  SPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDDQVLVILTVTG 780
            SPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDDQVLVILTVTG
Sbjct: 721  SPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDDQVLVILTVTG 780

Query: 781  QLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVAVRGASIYVLG 840
            QLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVAVRGASIYVLG
Sbjct: 781  QLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVAVRGASIYVLG 840

Query: 841  PMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQELVMPF 900
            PMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQELVMPF
Sbjct: 841  PMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQELVMPF 900

Query: 901  LIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVGGVAVEFCV 960
            LIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVGGVAVEFCV
Sbjct: 901  LIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVGGVAVEFCV 960

Query: 961  HISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQ 1020
            HISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQ
Sbjct: 961  HISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQ 1020

Query: 1021 RVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLRTSKSK 1080
            RVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLRTSKSK
Sbjct: 1021 RVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLRTSKSK 1080

Query: 1081 HASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVDTRSISNK 1140
            HASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVDTRSISNK
Sbjct: 1081 HASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVDTRSISNK 1140

Query: 1141 SSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQEEKNSISGR 1200
            SSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQEEKNSISGR
Sbjct: 1141 SSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQEEKNSISGR 1200

Query: 1201 KNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIATYVACGKA 1260
            KNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIATYVACGKA
Sbjct: 1201 KNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIATYVACGKA 1260

Query: 1261 TVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVIPETHWNPS 1320
            TVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVIPETHWNPS
Sbjct: 1261 TVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVIPETHWNPS 1320

Query: 1321 SVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLELGNSEQTE 1380
            SVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLELGNSEQTE
Sbjct: 1321 SVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLELGNSEQTE 1380

Query: 1381 FRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKTLIEVHLSG 1440
            FRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKTLIEVHLSG
Sbjct: 1381 FRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKTLIEVHLSG 1440

Query: 1441 SPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDIIELYVELLCQHER 1500
            SPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDIIELYVELLCQHER
Sbjct: 1441 SPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDIIELYVELLCQHER 1500

Query: 1501 ESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFHDLE 1560
            ESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFHDLE
Sbjct: 1501 ESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFHDLE 1560

Query: 1561 AAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLNSEESQTLW 1620
            AAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLNSEESQTLW
Sbjct: 1561 AAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLNSEESQTLW 1620

Query: 1621 FKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKSNKVAHLLR 1680
            FKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKSNKVAHLLR
Sbjct: 1621 FKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKSNKVAHLLR 1680

Query: 1681 KLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGFERRILDSA 1740
            KLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGFERRILDSA
Sbjct: 1681 KLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGFERRILDSA 1740

Query: 1741 KALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCGHATHLQCE 1800
            KALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCGHATHLQCE
Sbjct: 1741 KALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCGHATHLQCE 1800

Query: 1801 DLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVSYPQETDLL 1860
            DLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVSYPQETDLL
Sbjct: 1801 DLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVSYPQETDLL 1860

Query: 1861 ELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLLVGESSGGR 1920
            ELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLLVGESSGGR
Sbjct: 1861 ELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLLVGESSGGR 1920

Query: 1921 EKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1961
            EKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS
Sbjct: 1921 EKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1960

BLAST of CsaV3_3G007340 vs. ExPASy TrEMBL
Match: A0A1S3AUY3 (vacuolar protein sorting-associated protein 8 homolog OS=Cucumis melo OX=3656 GN=LOC103483112 PE=3 SV=1)

HSP 1 Score: 3608.5 bits (9356), Expect = 0.0e+00
Identity = 1856/1968 (94.31%), Postives = 1887/1968 (95.88%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSSGGDDDDDDDLSFPHRSIDEILNDSSSSTSPSP 60
            MTEELTDT TLPPMELDLNAFIHAHLSS GDDDDDDDLSFPHRSIDEILNDSSSSTS SP
Sbjct: 1    MTEELTDTRTLPPMELDLNAFIHAHLSS-GDDDDDDDLSFPHRSIDEILNDSSSSTSSSP 60

Query: 61   SSSPHFPPPRGRRNIVAGDDGVSASPSTSPY--------KDSEAARNNPWNEKSAQLKPG 120
            SSSPH PP RGRRNIVAG+ GVSASPSTSP+        KDSEA RNNPWNEKSAQ KPG
Sbjct: 61   SSSPHSPPSRGRRNIVAGNGGVSASPSTSPFKSLLEETIKDSEAPRNNPWNEKSAQSKPG 120

Query: 121  TASHSKVGELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSR 180
              SHSK+GELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSR
Sbjct: 121  KVSHSKIGELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSR 180

Query: 181  RAGYGNMVLDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNV 240
            RAGYGNM LDDDELASSSAVDSEF SDSLYH NIH KESGENSISVVDRITDYQ+AS +V
Sbjct: 181  RAGYGNMALDDDELASSSAVDSEFLSDSLYHTNIHLKESGENSISVVDRITDYQVASRDV 240

Query: 241  SGELWATNNIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGP 300
            S ELW  NNIRD VPHNDEFRMTEDMEFEAE SSVDDVNF ESL+TVPP ETNDRSLLGP
Sbjct: 241  S-ELWDRNNIRDSVPHNDEFRMTEDMEFEAEPSSVDDVNFNESLTTVPPAETNDRSLLGP 300

Query: 301  AEKNVCSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSKDT 360
            AEKNVCSTDAHPTELDVDESNEGAIPR TEPDDE SAVGYGS ELETQDFEKYHQPSKDT
Sbjct: 301  AEKNVCSTDAHPTELDVDESNEGAIPRSTEPDDEGSAVGYGSPELETQDFEKYHQPSKDT 360

Query: 361  EVDLAIEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFTAL 420
            EVDLAIEDPSIVNDIIESGETTEQ DNLQIGK PE + VSSTNPL+LAEEIEKKQAFTAL
Sbjct: 361  EVDLAIEDPSIVNDIIESGETTEQLDNLQIGKHPETMPVSSTNPLELAEEIEKKQAFTAL 420

Query: 421  HWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYI 480
            HWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYI
Sbjct: 421  HWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYI 480

Query: 481  AVGMSKGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGYSD 540
            AVGMSKG+IVVVASKYSAQNGDNMDAKMILLGSQGDKSTAP TSLCFSQQ DLLLAGYSD
Sbjct: 481  AVGMSKGSIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPVTSLCFSQQADLLLAGYSD 540

Query: 541  GHITVWDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVV 600
            GHITVWDVLRASAAKVISGEH SPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVV
Sbjct: 541  GHITVWDVLRASAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVV 600

Query: 601  PLLNRFSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVV 660
            PLLNRFS KTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVV
Sbjct: 601  PLLNRFSIKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVV 660

Query: 661  GGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYTAW 720
            GGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSP+VEVYAQLSKPDGIREGSMPYTAW
Sbjct: 661  GGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPSVEVYAQLSKPDGIREGSMPYTAW 720

Query: 721  KCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDDQV 780
            KCSQSFETS SEAVERVSLLAIAWDKMVQVAKLVKTELKVCG WSLESAAIGVVWLDDQV
Sbjct: 721  KCSQSFETSSSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGNWSLESAAIGVVWLDDQV 780

Query: 781  LVILTVTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVAVR 840
            LVILTVTGQLFLFEKDGTMIHQTS+F DGFVKEDFIAYHTHFAN+LG+PEKAYHNCVAVR
Sbjct: 781  LVILTVTGQLFLFEKDGTMIHQTSVFADGFVKEDFIAYHTHFANVLGHPEKAYHNCVAVR 840

Query: 841  GASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLES 900
            GASIYVLGP HLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLES
Sbjct: 841  GASIYVLGPTHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLES 900

Query: 901  LQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVG 960
            LQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDD+T E  SAHSEIKEQYNRVG
Sbjct: 901  LQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDVTSERDSAHSEIKEQYNRVG 960

Query: 961  GVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEH 1020
            GVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEH
Sbjct: 961  GVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEH 1020

Query: 1021 YSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLA 1080
            YSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLA
Sbjct: 1021 YSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLA 1080

Query: 1081 VLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAV 1140
            VLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAV
Sbjct: 1081 VLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAV 1140

Query: 1141 DTRSISNKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQE 1200
            DTRSISNKSSEVGCLNLY LLELDTEATLDVLRCAFVE E LK  SSLDGPVD  M+LQ+
Sbjct: 1141 DTRSISNKSSEVGCLNLYHLLELDTEATLDVLRCAFVEVEFLKTNSSLDGPVDAIMELQD 1200

Query: 1201 EKNSISGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIA 1260
            EKNSISGRKNFLIQNVVDALVHVL KAICETDESP GDNITLVDDWPSKKELIHLFDFIA
Sbjct: 1201 EKNSISGRKNFLIQNVVDALVHVLGKAICETDESPDGDNITLVDDWPSKKELIHLFDFIA 1260

Query: 1261 TYVACGKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVI 1320
            TYVACGKATVSKDVVGQILEHLISN+ IPET SDFLPRVTANSV SRKREKQVLSLLEV+
Sbjct: 1261 TYVACGKATVSKDVVGQILEHLISNTHIPET-SDFLPRVTANSVHSRKREKQVLSLLEVV 1320

Query: 1321 PETHWNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLE 1380
            PETHWNPSSVLRMCEKAQFFQVCGLIHSI  QYSSALDSYMKDV EPIH F FINR LL+
Sbjct: 1321 PETHWNPSSVLRMCEKAQFFQVCGLIHSIGCQYSSALDSYMKDVGEPIHAFAFINRALLK 1380

Query: 1381 LGNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKT 1440
            L NSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFN+DVSNIL QLRNHPRSLFLYLKT
Sbjct: 1381 LSNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNDDVSNILLQLRNHPRSLFLYLKT 1440

Query: 1441 LIEVHLSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDIIELYV 1500
            LIEVHLSGS DFSCLKKDDNLGVNYSTKG+DDYL+KLSDFPKYLSNNPVDVTDDIIELYV
Sbjct: 1441 LIEVHLSGSLDFSCLKKDDNLGVNYSTKGLDDYLKKLSDFPKYLSNNPVDVTDDIIELYV 1500

Query: 1501 ELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSL 1560
            ELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSL
Sbjct: 1501 ELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSL 1560

Query: 1561 DKKFHDLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLN 1620
            DKKFHDLEAAVGA VSN ASSGS+DSQ+F+SVLKLQEVN V+VLLHACIGLCQRNTPRLN
Sbjct: 1561 DKKFHDLEAAVGAIVSNGASSGSSDSQHFDSVLKLQEVNTVEVLLHACIGLCQRNTPRLN 1620

Query: 1621 SEESQTLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKS 1680
             EES+TLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNE S SQKDKEANIVTWRILKS
Sbjct: 1621 CEESETLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNEPSSSQKDKEANIVTWRILKS 1680

Query: 1681 NKVAHLLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGF 1740
            NK AH+LRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGF
Sbjct: 1681 NKAAHILRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGF 1740

Query: 1741 ERRILDSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCG 1800
            ERRILD+AKALIEDDSFYTM+LLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCG
Sbjct: 1741 ERRILDTAKALIEDDSFYTMNLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCG 1800

Query: 1801 HATHLQCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVS 1860
            HATHLQCEDLENEASGGD TCPICVHSNQSQGSKSKAPTEYSLVNKFSSRT SSSGASVS
Sbjct: 1801 HATHLQCEDLENEASGGDSTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTSSSSGASVS 1860

Query: 1861 YPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLL 1920
            YPQETD+LELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLL
Sbjct: 1861 YPQETDILELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLL 1920

Query: 1921 VGESSGGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1961
            VGESS GREKVEKLNKSRQLT VKVKRPSSLRFPLK SLFGKEKMTNS
Sbjct: 1921 VGESSSGREKVEKLNKSRQLTEVKVKRPSSLRFPLKASLFGKEKMTNS 1965

BLAST of CsaV3_3G007340 vs. ExPASy TrEMBL
Match: A0A6J1EC21 (vacuolar protein sorting-associated protein 8 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431840 PE=3 SV=1)

HSP 1 Score: 3293.1 bits (8537), Expect = 0.0e+00
Identity = 1702/1970 (86.40%), Postives = 1798/1970 (91.27%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSSGGDDDDD--DDLSFPHRSIDEILNDSSSSTSP 60
            MT+ELTDTETLPPMELDLNAFIHAHLSSG DDDDD  DDLSFPHRSIDEILN+SSSSTS 
Sbjct: 1    MTKELTDTETLPPMELDLNAFIHAHLSSGDDDDDDDEDDLSFPHRSIDEILNESSSSTSS 60

Query: 61   SPSSSPHFPPPRGRRNIVAGDDGVSASPSTSPYKD--------SEAARNNPWNEKSAQLK 120
            SPSS P+ PPPR  R+I A D   SAS S  P+K         S+  R+N  NEKS QLK
Sbjct: 61   SPSSPPNSPPPRDHRSIAARDGRASASRSIPPFKSPFEEIIKASKVPRSNQRNEKSVQLK 120

Query: 121  PGTASHSKVGELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIK 180
            PG+ SH+KVGELTDDPFRRGSR LPSLFG VRSNAKPGAALAAAAAASRS PAPHAAAIK
Sbjct: 121  PGSVSHTKVGELTDDPFRRGSRALPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIK 180

Query: 181  SRRAGYGNMVLDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASM 240
            SRR+G+G++VLDDDELASSSAVDSEF  D LY    HSKES E SIS+V+R  DYQ AS+
Sbjct: 181  SRRSGHGSVVLDDDELASSSAVDSEFVFDDLYPTIDHSKESREKSISLVERNADYQGASV 240

Query: 241  NVSGELWATNNIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLL 300
            NV GE WA +NIRD V +NDEFR+T+D E EAE S VDDVNF ES +T+PPVE N RSL 
Sbjct: 241  NVGGEFWARDNIRDCVLYNDEFRITKDTECEAEQSFVDDVNFDESSTTLPPVEANGRSLS 300

Query: 301  GPAEKNVCSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSK 360
              A+ NVCS DA PT LDVDESNEGA P   +PD + SAVGYG LELETQDFEK  QPSK
Sbjct: 301  DSADNNVCSMDAEPTVLDVDESNEGAFPSSPKPDYDRSAVGYGRLELETQDFEKRFQPSK 360

Query: 361  DTEVDLAIEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFT 420
            D+EV LAIED S+VNDI ES ET EQ DN   G+R E +S+SS+NPL+LAEEIEKKQAFT
Sbjct: 361  DSEV-LAIEDLSVVNDISESRETAEQLDNFHTGERAETMSLSSSNPLELAEEIEKKQAFT 420

Query: 421  ALHWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHAN 480
            ALHWEEGVAAQPMRLEGIKG TT LGYFDIQADNSISRTISSHSF+REHGFPQ LAVHAN
Sbjct: 421  ALHWEEGVAAQPMRLEGIKGGTTALGYFDIQADNSISRTISSHSFKREHGFPQALAVHAN 480

Query: 481  YIAVGMSKGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGY 540
            YIAVGMSKGNIVVVASKYSAQNGDNMDAKM+LLGSQGDKSTAP TSLCF+QQGDLLLAGY
Sbjct: 481  YIAVGMSKGNIVVVASKYSAQNGDNMDAKMLLLGSQGDKSTAPVTSLCFNQQGDLLLAGY 540

Query: 541  SDGHITVWDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS 600
            SDG +TVWDVLRA+AAKVISGEH SPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS
Sbjct: 541  SDGQVTVWDVLRATAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS 600

Query: 601  VVPLLNRFSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGG 660
            VVPLLNRFS KTQCLLDGQKTGTVLSASALLLNEF GSSLPP+LSNVAVSTSSIGSMMGG
Sbjct: 601  VVPLLNRFSIKTQCLLDGQKTGTVLSASALLLNEFCGSSLPPSLSNVAVSTSSIGSMMGG 660

Query: 661  VVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYT 720
            VVGGDSGWKLFNEGSSLV EGVVIFATHQTALVVRLSPTVEVYA+LSKPDGI+EGSMPYT
Sbjct: 661  VVGGDSGWKLFNEGSSLV-EGVVIFATHQTALVVRLSPTVEVYARLSKPDGIQEGSMPYT 720

Query: 721  AWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDD 780
            AWKCSQS ETSPSEAVER+SLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGV WLDD
Sbjct: 721  AWKCSQSIETSPSEAVERISLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVAWLDD 780

Query: 781  QVLVILTVTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVA 840
            QVLVILTVTGQLFLFEKDGTMIHQTS+FVDGF KEDFIA+HTHF N+LGNPEKAYHNCVA
Sbjct: 781  QVLVILTVTGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAHHTHFVNVLGNPEKAYHNCVA 840

Query: 841  VRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSL 900
            VRGAS+YVLGP HLVISRLLPWKERVQVLRKAGDWM+ALSMAITIYDGHAHGVIDLPRSL
Sbjct: 841  VRGASVYVLGPKHLVISRLLPWKERVQVLRKAGDWMTALSMAITIYDGHAHGVIDLPRSL 900

Query: 901  ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNR 960
            ESLQELVMPFLIELLLSYVDEVFSYIS+AFCNQIEKNEKLDD+T  SHSAHSEIKEQYNR
Sbjct: 901  ESLQELVMPFLIELLLSYVDEVFSYISLAFCNQIEKNEKLDDVTSGSHSAHSEIKEQYNR 960

Query: 961  VGGVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020
            VGGVAVEFCVHI+RTDILFDEIFSKFV VQQRDTFLELLEPYILKDMLGSLPPEIMQALV
Sbjct: 961  VGGVAVEFCVHITRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020

Query: 1021 EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080
            EHYS KGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL
Sbjct: 1021 EHYSQKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080

Query: 1081 LAVLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD 1140
            LAVL+TSKSK AS++GYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD
Sbjct: 1081 LAVLQTSKSKRASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD 1140

Query: 1141 AVDTRSISNKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQL 1200
             VDTRSISNKSSEVG LNLY LLELDT ATLDVLRCAFVEGEI+KA SSLDG VD SMQ+
Sbjct: 1141 TVDTRSISNKSSEVGYLNLYHLLELDTGATLDVLRCAFVEGEIVKADSSLDGSVDASMQV 1200

Query: 1201 QEEKNSISGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDF 1260
            Q+E       KNFL+QNVVDAL+H+LDKAI +T  SP GDNITLV+DWPSKK+L HLFDF
Sbjct: 1201 QKE-------KNFLVQNVVDALIHILDKAISQTYGSPGGDNITLVEDWPSKKDLFHLFDF 1260

Query: 1261 IATYVACGKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLE 1320
            +A YVACGKATVSKDVVGQILEHLISNSDIPE   DF+  VTANSV SRKREKQVLSLLE
Sbjct: 1261 VANYVACGKATVSKDVVGQILEHLISNSDIPEMEIDFVHSVTANSVHSRKREKQVLSLLE 1320

Query: 1321 VIPETHWNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTL 1380
            VIPETHWNPSSVLRMCEKAQFFQVCGLIHSI+HQYSSALD YMKDV+EPIH F FINRTL
Sbjct: 1321 VIPETHWNPSSVLRMCEKAQFFQVCGLIHSISHQYSSALDGYMKDVEEPIHAFAFINRTL 1380

Query: 1381 LELGNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYL 1440
            +EL  SEQTEFR VVISRIPEL NLNR  TFFLVIDHF NDVS+ILSQL NHPRSLFLYL
Sbjct: 1381 MELSYSEQTEFRGVVISRIPELLNLNREGTFFLVIDHFGNDVSDILSQLHNHPRSLFLYL 1440

Query: 1441 KTLIEVHLSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDIIEL 1500
            KTLIEVH SG+ +FSCLKKDDN GVNYSTKG+DDYLQKLSDFPK+LSNNPVDVTDDIIEL
Sbjct: 1441 KTLIEVHQSGNLNFSCLKKDDNFGVNYSTKGLDDYLQKLSDFPKFLSNNPVDVTDDIIEL 1500

Query: 1501 YVELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560
            YVELLCQ+ERESVLKFLETFDSY VEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS
Sbjct: 1501 YVELLCQYERESVLKFLETFDSYHVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560

Query: 1561 SLDKKFHDLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPR 1620
            SLDKKFHDLEAAVG  V+N ASSGSNDSQ F+SVLKLQEVN + VLLHACIGLCQRNTPR
Sbjct: 1561 SLDKKFHDLEAAVGGIVTNGASSGSNDSQLFSSVLKLQEVNDIYVLLHACIGLCQRNTPR 1620

Query: 1621 LNSEESQTLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRIL 1680
            LNSEES+TLWFKLLDSFCEPLI+S+N+RTASF +NQVQFLNESS SQKDKEA+IVTWRIL
Sbjct: 1621 LNSEESETLWFKLLDSFCEPLIESFNYRTASFGENQVQFLNESSGSQKDKEAHIVTWRIL 1680

Query: 1681 KSNKVAHLLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTF 1740
            KSN+ AH+LR LFS+FIREIVEGM+GYVHLPTIMSRLL DNGSQEFGDFKLTILGMLGTF
Sbjct: 1681 KSNQTAHILRSLFSRFIREIVEGMIGYVHLPTIMSRLLSDNGSQEFGDFKLTILGMLGTF 1740

Query: 1741 GFERRILDSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFN 1800
            GFERRILD+AKALIEDD+FYTMSLLKKGA+HGYAPR  VCCICNRLLVKSSSSYRVRVFN
Sbjct: 1741 GFERRILDTAKALIEDDTFYTMSLLKKGASHGYAPRGAVCCICNRLLVKSSSSYRVRVFN 1800

Query: 1801 CGHATHLQCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGAS 1860
            CGHATHLQCE L+NEASGGD++CP+CVHSN SQ S+ KA TEYSLVNKFSSRTQSSSGA 
Sbjct: 1801 CGHATHLQCEVLDNEASGGDFSCPVCVHSNHSQRSRGKALTEYSLVNKFSSRTQSSSGAF 1860

Query: 1861 VSYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYH 1920
            VSYPQETDLLELPYTLQQIPRFEIL NLQKNQRVIDIEN+PQLRLAPPAVYHDKVTKGYH
Sbjct: 1861 VSYPQETDLLELPYTLQQIPRFEILANLQKNQRVIDIENMPQLRLAPPAVYHDKVTKGYH 1920

Query: 1921 LLVGESSGGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1961
            LLVG+SSGG EKVEKLNKSRQL  VKVKRPSSLRFPLK +LFGKEK T S
Sbjct: 1921 LLVGDSSGGIEKVEKLNKSRQLKEVKVKRPSSLRFPLKANLFGKEKTTKS 1961

BLAST of CsaV3_3G007340 vs. ExPASy TrEMBL
Match: A0A6J1IVQ0 (vacuolar protein sorting-associated protein 8 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479042 PE=3 SV=1)

HSP 1 Score: 3284.6 bits (8515), Expect = 0.0e+00
Identity = 1695/1968 (86.13%), Postives = 1796/1968 (91.26%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSSGGDDDDDDDLSFPHRSIDEILNDSSSSTSPSP 60
            MT++LTDTETLPPMELDLNAFIHAHLSS GDDDD+DDLSFPHRSIDEILN+SSSSTS SP
Sbjct: 1    MTKQLTDTETLPPMELDLNAFIHAHLSS-GDDDDEDDLSFPHRSIDEILNESSSSTSSSP 60

Query: 61   SSSPHFPPPRGRRNIVAGDDGVSASPSTSPYKD--------SEAARNNPWNEKSAQLKPG 120
            SS P+ PPPR RR+I A D   SAS S  P+K         S+  R+N  NEKS QLKPG
Sbjct: 61   SSPPNLPPPRARRSIAARDGRASASHSIPPFKSPFEEIIKASKVPRSNQRNEKSVQLKPG 120

Query: 121  TASHSKVGELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSR 180
            + SH+KVGELTDDPFRRGSR LPSLFG VRSNAKPGAALAAAAAASRS PAPHAAAIKSR
Sbjct: 121  SVSHTKVGELTDDPFRRGSRALPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIKSR 180

Query: 181  RAGYGNMVLDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNV 240
            R+G+G++VLDDDELASSSAVDSEF  D LY    HSKES E SIS+V+R  DYQ AS   
Sbjct: 181  RSGHGSVVLDDDELASSSAVDSEFVFDDLYPTIDHSKESREKSISLVERNADYQGAS--- 240

Query: 241  SGELWATNNIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGP 300
             GE WA +NIRD V +NDEFR+T+D E EAE S VDDVNF ES +T+PPVE N RSL   
Sbjct: 241  GGEFWARDNIRDCVLYNDEFRITKDTECEAEQSFVDDVNFNESSTTLPPVEANGRSLSDS 300

Query: 301  AEKNVCSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSKDT 360
            A+ NVCS DA PT LDVDESNEGA     +PD ++SAVGYG LELETQDFEK  QPSKD+
Sbjct: 301  ADNNVCSMDAEPTVLDVDESNEGAFSCSPKPDYDKSAVGYGRLELETQDFEKRFQPSKDS 360

Query: 361  EVDLAIEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFTAL 420
            EV LAIED S+VNDI ES ET EQ DN   G+R E +S+SSTNPL+LAEEIEKKQAFTAL
Sbjct: 361  EV-LAIEDLSVVNDISESRETAEQLDNFDTGERAETMSLSSTNPLELAEEIEKKQAFTAL 420

Query: 421  HWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYI 480
            HWEEGVAAQPMRLEGIKG TT LGYFDIQADN ISRTISSHSF+REHGFPQVLAVH NYI
Sbjct: 421  HWEEGVAAQPMRLEGIKGGTTALGYFDIQADNCISRTISSHSFKREHGFPQVLAVHTNYI 480

Query: 481  AVGMSKGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGYSD 540
            AVGMSKGNIVVVASKYSAQNGDNMD KM+LLGSQGDKSTAP TSLCF+QQGDLLLAGYSD
Sbjct: 481  AVGMSKGNIVVVASKYSAQNGDNMDGKMLLLGSQGDKSTAPVTSLCFNQQGDLLLAGYSD 540

Query: 541  GHITVWDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVV 600
            G +TVWDVLRA+AAKVISGEH SPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVV
Sbjct: 541  GQVTVWDVLRATAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVV 600

Query: 601  PLLNRFSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVV 660
            PLLNRFS KTQCLLDGQKTGTVLSASALLLNEF GSSLPP+LSNVAVSTSSIGSMMGGVV
Sbjct: 601  PLLNRFSIKTQCLLDGQKTGTVLSASALLLNEFCGSSLPPSLSNVAVSTSSIGSMMGGVV 660

Query: 661  GGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYTAW 720
            GGDSGWKLFNEGSSLV EGVVIFATHQTALVVRLSPTVEVYA+LSKPDGI+EGSMPYTAW
Sbjct: 661  GGDSGWKLFNEGSSLV-EGVVIFATHQTALVVRLSPTVEVYARLSKPDGIQEGSMPYTAW 720

Query: 721  KCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDDQV 780
            KCSQS ETSPSEAVER+SLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGV WLDDQV
Sbjct: 721  KCSQSIETSPSEAVERISLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVAWLDDQV 780

Query: 781  LVILTVTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVAVR 840
            LVILTVTGQLFLFEKDGTMIHQTS+FVDGF KEDFIA+HTHF N+LGNPEKAYHNCVAVR
Sbjct: 781  LVILTVTGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAHHTHFVNVLGNPEKAYHNCVAVR 840

Query: 841  GASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLES 900
            GAS+YVLGP HLVISRLLPWKERVQVLRKAGDWM+ALSMAITIYDGHAHGVIDLPRSLES
Sbjct: 841  GASVYVLGPKHLVISRLLPWKERVQVLRKAGDWMTALSMAITIYDGHAHGVIDLPRSLES 900

Query: 901  LQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVG 960
            LQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDD+T  SHS HSEIKEQYNRVG
Sbjct: 901  LQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDVTSGSHSEHSEIKEQYNRVG 960

Query: 961  GVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEH 1020
            GVAVEFCVHI+RTDILFDEIFSKFV VQQRDTFLELLEPYILKDMLGSLPPEIMQALVEH
Sbjct: 961  GVAVEFCVHITRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEH 1020

Query: 1021 YSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLA 1080
            YS +GWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLA
Sbjct: 1021 YSQRGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLA 1080

Query: 1081 VLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAV 1140
            VL+TSKSK AS++GYK LVYLKYCFSGLAFPPGQGTL+HSRVQSLRDELLQFLLENSD V
Sbjct: 1081 VLQTSKSKRASAIGYKILVYLKYCFSGLAFPPGQGTLSHSRVQSLRDELLQFLLENSDTV 1140

Query: 1141 DTRSISNKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQE 1200
            DTRSISNKSSEVG LNLY LL+LDT ATLDVLRCAFVEGEI+KA SSLDG VD S+Q Q+
Sbjct: 1141 DTRSISNKSSEVGYLNLYHLLKLDTGATLDVLRCAFVEGEIVKADSSLDGSVDASIQAQK 1200

Query: 1201 EKNSISGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIA 1260
            EKNS SGRKNFL+QNVVDALVH+LDKAI +T  SP GDNITLV+DWPSKK L HLFDF+A
Sbjct: 1201 EKNSTSGRKNFLVQNVVDALVHILDKAISQTYRSPGGDNITLVEDWPSKKNLFHLFDFVA 1260

Query: 1261 TYVACGKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVI 1320
             YV CGKAT SKDVVGQILEHLISNSDIPE   DF+  VTANSV SRKRE+QVLSLLEVI
Sbjct: 1261 NYVVCGKATASKDVVGQILEHLISNSDIPEMEIDFVHSVTANSVHSRKRERQVLSLLEVI 1320

Query: 1321 PETHWNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLE 1380
            PETHWNPSSVLRMCEKAQFFQVCGLIHSI+HQYSSALD YMKDVDEPIH F FINRTL+E
Sbjct: 1321 PETHWNPSSVLRMCEKAQFFQVCGLIHSISHQYSSALDGYMKDVDEPIHAFAFINRTLME 1380

Query: 1381 LGNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKT 1440
            L NSEQTEFR VVISRIPEL NLNR  TFFLVIDHF+NDVS+ILSQL NHPRSLFLYLKT
Sbjct: 1381 LNNSEQTEFRGVVISRIPELLNLNREGTFFLVIDHFSNDVSDILSQLHNHPRSLFLYLKT 1440

Query: 1441 LIEVHLSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDIIELYV 1500
            LIEVH SG+ +FSCLKKDDN GVNYSTKG+DDYLQKLSDFPK+LSNNPVDVTDDIIELYV
Sbjct: 1441 LIEVHQSGNLNFSCLKKDDNFGVNYSTKGLDDYLQKLSDFPKFLSNNPVDVTDDIIELYV 1500

Query: 1501 ELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSL 1560
            ELLCQ+ERESVLKFLETFDSY VEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSL
Sbjct: 1501 ELLCQYERESVLKFLETFDSYHVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSL 1560

Query: 1561 DKKFHDLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLN 1620
            DKKFHDLEAAVG  V+N ASSGSNDSQ F+SVLKLQEVN + VLLHACIGLCQRNTPRLN
Sbjct: 1561 DKKFHDLEAAVGGIVTNGASSGSNDSQLFSSVLKLQEVNDIYVLLHACIGLCQRNTPRLN 1620

Query: 1621 SEESQTLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKS 1680
            SEES+TLWFKLLDSFCEPLI+S+N+RTASF +NQVQFLNESS SQKDKEA+IVTWRILKS
Sbjct: 1621 SEESETLWFKLLDSFCEPLIESFNYRTASFGENQVQFLNESSGSQKDKEAHIVTWRILKS 1680

Query: 1681 NKVAHLLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGF 1740
            N+ AH+LR LFS+FIREIVEGM+GYVHLPTIMSRLL DNGSQEFGDFKLTILGMLGTFGF
Sbjct: 1681 NQTAHILRSLFSRFIREIVEGMIGYVHLPTIMSRLLSDNGSQEFGDFKLTILGMLGTFGF 1740

Query: 1741 ERRILDSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCG 1800
            ERRILD+AKALIEDD+FYTMSLLKKGA+HGYAPR  VCCICNRLLVKSSSSYRVRVFNCG
Sbjct: 1741 ERRILDTAKALIEDDTFYTMSLLKKGASHGYAPRGAVCCICNRLLVKSSSSYRVRVFNCG 1800

Query: 1801 HATHLQCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVS 1860
            HATHLQCE L+NEASGGD++C +CVHSN SQ S+ KA TEYSLVNKFSSRTQSSSGASVS
Sbjct: 1801 HATHLQCEVLDNEASGGDFSCQVCVHSNHSQRSRGKALTEYSLVNKFSSRTQSSSGASVS 1860

Query: 1861 YPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLL 1920
            YPQETDLLELPYTLQQIPRFEIL NLQKNQRVIDIEN+PQLRLAPPAVYHDKVTKG+HLL
Sbjct: 1861 YPQETDLLELPYTLQQIPRFEILANLQKNQRVIDIENMPQLRLAPPAVYHDKVTKGFHLL 1920

Query: 1921 VGESSGGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1961
            VG+SSGG EKVEKLNKSRQL  VKVK+PSSLRFPLK +LFGKEK + S
Sbjct: 1921 VGDSSGGVEKVEKLNKSRQLKEVKVKKPSSLRFPLKANLFGKEKTSKS 1962

BLAST of CsaV3_3G007340 vs. ExPASy TrEMBL
Match: A0A6J1CRK3 (vacuolar protein sorting-associated protein 8 homolog OS=Momordica charantia OX=3673 GN=LOC111013938 PE=3 SV=1)

HSP 1 Score: 2983.0 bits (7732), Expect = 0.0e+00
Identity = 1548/1960 (78.98%), Postives = 1664/1960 (84.90%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSSGGDDDDDDDLSFPHRSIDEILNDSSSSTSPSP 60
            MT+ELTDT  LP MELDLNAFIHAHLSS GDDDDDDD SFPHR+IDEIL +S S     P
Sbjct: 1    MTKELTDTGVLPLMELDLNAFIHAHLSS-GDDDDDDDPSFPHRTIDEILKESGS----PP 60

Query: 61   SSSPHFPPPRGRRNIVAGDDGVSASPSTSPYKDSEAARNNPWNEKSAQLKPGTASHSKVG 120
            SS PH PP  G R +   D                                         
Sbjct: 61   SSPPHSPPYGGDRAVATRD----------------------------------------- 120

Query: 121  ELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRRAGYGNMV 180
                                                                    GN  
Sbjct: 121  --------------------------------------------------------GNST 180

Query: 181  LDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNVSGELWATN 240
            + DD  A                                    DYQ+AS+NV   +WA +
Sbjct: 181  VMDDTDA------------------------------------DYQVASINVGDGVWARD 240

Query: 241  NIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGPAEKNVCST 300
             IRD VP NDEFR+T+ ++  AE  SVDDVNF ES + +PPVE ND S+   AEKNVCST
Sbjct: 241  RIRDDVPDNDEFRITKGLKCVAEQRSVDDVNFDESTTILPPVEANDGSVSDYAEKNVCST 300

Query: 301  DAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSKDTEVDLAIED 360
            +  P+ LDVDESNEGAIP  +EPD++ SAV YGSLE ETQDF+K  Q SKDTEVDL I+D
Sbjct: 301  ELRPSILDVDESNEGAIPSSSEPDNDGSAVDYGSLEWETQDFKKCLQQSKDTEVDLVIKD 360

Query: 361  PSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFTALHWEEGVAA 420
            PS+VNDI E GETTE  D+  I ++ EM+ + STNPL+ AEEIEKKQAFTALHWEEGVAA
Sbjct: 361  PSVVNDISELGETTEHMDDSTISEQAEMMLLPSTNPLEAAEEIEKKQAFTALHWEEGVAA 420

Query: 421  QPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYIAVGMSKGN 480
            QPM+LEGIKG +TTLGYFDIQADNSISRTISSHSF+REHGFPQVLAVHANYIAVGMSKGN
Sbjct: 421  QPMKLEGIKGGSTTLGYFDIQADNSISRTISSHSFKREHGFPQVLAVHANYIAVGMSKGN 480

Query: 481  IVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGYSDGHITVWDV 540
            IVVVASKYSAQNGDNMDAKM+LLG QGDKSTAP TSLCF+ QGDLLLAGY+DGHITVWDV
Sbjct: 481  IVVVASKYSAQNGDNMDAKMLLLGLQGDKSTAPVTSLCFNLQGDLLLAGYNDGHITVWDV 540

Query: 541  LRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSS 600
            LRASAAK+ISGEH SPVVHSLFLGQ+AQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFS 
Sbjct: 541  LRASAAKIISGEHTSPVVHSLFLGQDAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSI 600

Query: 601  KTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVVGGDSGWKL 660
            KTQCLLDGQKTGTVLSASALLLNE  GSSLPPTL+NVAVSTSSIGSMMGGVVGGDSGWKL
Sbjct: 601  KTQCLLDGQKTGTVLSASALLLNESCGSSLPPTLANVAVSTSSIGSMMGGVVGGDSGWKL 660

Query: 661  FNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYTAWKCSQSFET 720
            FNEGSSLVEEGVVIFATHQTALVVRLSPT+EVYAQLSKPDG+REGSMPYTAWKCSQSFET
Sbjct: 661  FNEGSSLVEEGVVIFATHQTALVVRLSPTLEVYAQLSKPDGVREGSMPYTAWKCSQSFET 720

Query: 721  SPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDDQVLVILTVTG 780
            SPSEAVERVSLLAIAWDKMVQVAKLVK ELKVCGKWSLESAAIGV WLDDQVLV+LT+TG
Sbjct: 721  SPSEAVERVSLLAIAWDKMVQVAKLVKAELKVCGKWSLESAAIGVAWLDDQVLVVLTITG 780

Query: 781  QLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVAVRGASIYVLG 840
            QLFLFEKDGTMIHQTS+FVDGF KEDFIAYHTHF N+LGNPEKAYHNCVAVRGAS+YVLG
Sbjct: 781  QLFLFEKDGTMIHQTSVFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVAVRGASVYVLG 840

Query: 841  PMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQELVMPF 900
            PMHL+ISRLLPWKERVQVLRKAGDWM ALSMAITIYDG AHGVIDLPRSLESLQELV+PF
Sbjct: 841  PMHLIISRLLPWKERVQVLRKAGDWMGALSMAITIYDGQAHGVIDLPRSLESLQELVVPF 900

Query: 901  LIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVGGVAVEFCV 960
            LIELLLSYVDEVFSYISVAFCNQIEKNEK  D+T   HSAHSEIKEQYNRVGGVAVEFCV
Sbjct: 901  LIELLLSYVDEVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSEIKEQYNRVGGVAVEFCV 960

Query: 961  HISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQ 1020
            HI RTDILFDEIFSKFV VQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQ
Sbjct: 961  HIRRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQ 1020

Query: 1021 RVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLRTSKSK 1080
            RVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVL+TSKS+
Sbjct: 1021 RVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLQTSKSE 1080

Query: 1081 HASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVDTRSISNK 1140
             AS++GYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD+VDTRSISNK
Sbjct: 1081 RASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDSVDTRSISNK 1140

Query: 1141 SSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQEEKNSISGR 1200
            SSE+G L LY LLELDT ATLDVLRCAFVEGEI K  SS +G  D SMQ+QEEKNS SGR
Sbjct: 1141 SSEIGYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSSPNGLDDVSMQVQEEKNSTSGR 1200

Query: 1201 KNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIATYVACGKA 1260
            KN LIQ++VDALVHVLD+AIC+TD S  G++I LV DWPSKK+L+HLFDF+A YVACG A
Sbjct: 1201 KNVLIQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWPSKKDLVHLFDFVANYVACGGA 1260

Query: 1261 TVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVIPETHWNPS 1320
            T SK +VGQILEHLISNSDIPET +DFLP+VTANSV SRKREKQVLSLLEV+PETHWN S
Sbjct: 1261 TASKVIVGQILEHLISNSDIPETENDFLPKVTANSVHSRKREKQVLSLLEVVPETHWNAS 1320

Query: 1321 SVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLELGNSEQTE 1380
            SVL MCE+AQFFQVCGLIHSI HQY+SALDSYMKD+DEPIH F FINRTLLEL N E+TE
Sbjct: 1321 SVLSMCEEAQFFQVCGLIHSIRHQYASALDSYMKDIDEPIHGFAFINRTLLELNNYERTE 1380

Query: 1381 FRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKTLIEVHLSG 1440
            F+AVVISRIPELFNLNR  TFFLVIDHFNND S+ILSQLR HPRSLFLYLKTLIEVHLSG
Sbjct: 1381 FQAVVISRIPELFNLNREGTFFLVIDHFNNDASDILSQLRTHPRSLFLYLKTLIEVHLSG 1440

Query: 1441 SPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDIIELYVELLCQHER 1500
            S +FSCL+KDD  G+NYSTKG+DDYL+KLSDFPKYLSNNPVDVTDDIIELYVELLCQ+ER
Sbjct: 1441 SLNFSCLRKDDIFGLNYSTKGLDDYLRKLSDFPKYLSNNPVDVTDDIIELYVELLCQYER 1500

Query: 1501 ESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFHDLE 1560
            ESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKF+DLE
Sbjct: 1501 ESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFNDLE 1560

Query: 1561 AAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLNSEESQTLW 1620
            AAV + V+N ASSGSND ++FNSVLKLQEVN + VLLHACIGLCQRNTPRLNSEES+TLW
Sbjct: 1561 AAVESIVANGASSGSNDPRHFNSVLKLQEVNDIDVLLHACIGLCQRNTPRLNSEESETLW 1620

Query: 1621 FKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKSNKVAHLLR 1680
            FKLLDSFCEPLIDSYN+RT +F +NQVQFLNESS  QKDKEA IVTWRILKS+K AH+L+
Sbjct: 1621 FKLLDSFCEPLIDSYNYRTPAFGENQVQFLNESSGLQKDKEARIVTWRILKSDKAAHILK 1680

Query: 1681 KLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGFERRILDSA 1740
            +LFS+FI+EIVEGM+GYVHLP IMSRLL DNGSQEFGDFKLT+LGMLGTFGFERRILD+A
Sbjct: 1681 RLFSRFIKEIVEGMIGYVHLPIIMSRLLSDNGSQEFGDFKLTVLGMLGTFGFERRILDTA 1740

Query: 1741 KALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCGHATHLQCE 1800
            KALIEDD+FYTMSLLKKGA+HGYAPR++VCCICN LL+KSSSSYRVRVFNCGHATHLQCE
Sbjct: 1741 KALIEDDTFYTMSLLKKGASHGYAPRNLVCCICNCLLIKSSSSYRVRVFNCGHATHLQCE 1800

Query: 1801 DLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVSYPQETDLL 1860
             L+NEASGGD++CP+C+HSNQSQ  + KA TEYSLVN FSS TQS SGASV YPQETDLL
Sbjct: 1801 VLDNEASGGDFSCPVCIHSNQSQRPRGKALTEYSLVNTFSSGTQSLSGASVQYPQETDLL 1819

Query: 1861 ELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLLVGESSGGR 1920
            ELPYTLQQIPRFEILTNLQKNQRVI+IENVPQLRLAPPAVYHDKVTKGYHLLVGESS G 
Sbjct: 1861 ELPYTLQQIPRFEILTNLQKNQRVIEIENVPQLRLAPPAVYHDKVTKGYHLLVGESSSG- 1819

Query: 1921 EKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1961
              +EK NKS+QL  VKVKRPSSLRFPLK SLFGKE+ T S
Sbjct: 1921 --IEKQNKSKQLREVKVKRPSSLRFPLKASLFGKERTTKS 1819

BLAST of CsaV3_3G007340 vs. TAIR 10
Match: AT4G00800.1 (transducin family protein / WD-40 repeat family protein )

HSP 1 Score: 1829.3 bits (4737), Expect = 0.0e+00
Identity = 1054/1973 (53.42%), Postives = 1351/1973 (68.47%), Query Frame = 0

Query: 14   MELDLNAFIHAHLSSGGDDDDDDDLSFPHRSIDEILNDSSSSTSPSPSSSPHFPPPRGRR 73
            MELDL++F+   +S    D D D  S PHR++DEILN SSSS+  + SS P  PP   RR
Sbjct: 1    MELDLDSFL---VSDSDSDSDLDSSSVPHRTVDEILNASSSSS--ASSSPPSSPPSINRR 60

Query: 74   NIVAGDDGVSASPSTSPYKDSEAARNNPWNEKSAQLKPGTASHSKVGELTDDPFRRGSR- 133
                 DD     P+    + SEA  N       A L+P +  H         P RR S  
Sbjct: 61   K---QDD-----PNR---RLSEALTN------VAVLRPESELHRGF-----PPTRRNSTS 120

Query: 134  -------PLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRRAG---------Y 193
                   PLPSL   VRSN KPGAALAAA AASR  P PHAA IKSRRA           
Sbjct: 121  SSSLRQLPLPSLLAGVRSNVKPGAALAAAVAASRLVPTPHAAIIKSRRASSASSELLLQV 180

Query: 194  GNMVLDDDELASSS------AVDSEFFSDSLYHANIHSKESGENSISVVDRITD-YQIAS 253
             N   DD E+ SS+      A  S    D          E  +N +S V  + D  ++  
Sbjct: 181  SNQEEDDHEVLSSNGDSVGVAAGSVSADDFRSFGGESLLEDEDNGVSGVASLEDEAKVME 240

Query: 254  MNVSGELWATNNIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSL 313
            +  S    + N     V    +       E EAET+        E+ +     +T++  L
Sbjct: 241  VQASDITESLNPDLVTVSSGFDSEGNVSTEKEAETT-------MEAGNAAIDDDTDETML 300

Query: 314  LGPAEKNVCSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPS 373
            +    ++  S   H T+      +EG        +DEES+VG    ++++   +     S
Sbjct: 301  VASLVES--SESQHLTD------SEGKCDDAKVSNDEESSVG----DVKSDKSDIIIPES 360

Query: 374  KDTEVDLAIEDP----SIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEK 433
            K    D  I D     S +++++E  E   + +N ++ KR  + S S    L LAEE EK
Sbjct: 361  KKEGGDAFIPDDGSSMSGISELVE--ERIAELENERMSKRERLKSQSFRKQLVLAEEFEK 420

Query: 434  KQAFTALHWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVL 493
            KQA+T LHWEEG AAQPMRLEG+K  +T LGYFD+ ADN ISRTISS +F+R+HG PQVL
Sbjct: 421  KQAYTGLHWEEGAAAQPMRLEGVKIGSTNLGYFDVDADNVISRTISSQAFKRDHGSPQVL 480

Query: 494  AVHANYIAVGMSKGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDL 553
            AVH NYIAVG SKG IVVV SKYS+ + D M++KMI LG QG++S +P TS+CF+Q G L
Sbjct: 481  AVHLNYIAVGTSKGVIVVVPSKYSSDHADQMESKMIWLGLQGERSQSPVTSVCFNQIGSL 540

Query: 554  LLAGYSDGHITVWDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVL 613
            LLAGY DGH+TVWD+ RAS AKVI+ EH +PVV++ FLG+++Q +RQFK +T D+KG+V 
Sbjct: 541  LLAGYGDGHVTVWDMQRASIAKVIT-EHTAPVVYAFFLGRDSQGSRQFKVITSDTKGVVF 600

Query: 614  LHTFSVVPLLNRFSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIG 673
             H+FS   LLN ++ +TQCLLDGQK GTVLSAS L    F  S +     N AV +SSI 
Sbjct: 601  KHSFSYARLLNMYTVETQCLLDGQKNGTVLSASPLPDENFGSSLVSSKGGNSAVPSSSIS 660

Query: 674  SMMGGVVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREG 733
            SMMGGVVG  S WKLFNE S+ VEEGVVIFAT+QT LVV+L P +EVYAQL +P+G+REG
Sbjct: 661  SMMGGVVGVGSTWKLFNEDSTSVEEGVVIFATYQTGLVVKLIPNLEVYAQLPRPEGVREG 720

Query: 734  SMPYTAWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGV 793
            SMPYTAW+  +S E    EA +RVS L IAWD+ VQVAKLVK+++K   KWSL+S AIGV
Sbjct: 721  SMPYTAWR--RSTENYSKEAEDRVSFLVIAWDRRVQVAKLVKSDIKEYAKWSLDSPAIGV 780

Query: 794  VWLDDQVLVILTVTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAY 853
            VWLDDQ+LVI TVTG L+LF +DG +IHQT+  V G    D I+YHT+F N+ GNPEKAY
Sbjct: 781  VWLDDQLLVIPTVTGHLYLFTRDGVVIHQTNFSVAGSSGNDLISYHTYFTNVFGNPEKAY 840

Query: 854  HNCVAVRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVID 913
            HN + VRGAS+Y+LG  HLVISRLLPWKERV VLR+ GDWM A +MA+++++G AHGV+D
Sbjct: 841  HNSMGVRGASVYILGTAHLVISRLLPWKERVDVLRRGGDWMGAFNMAMSLFNGQAHGVVD 900

Query: 914  LPRSLESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIK 973
            LP+++++++E + P L ELLLSYVDEVFSYIS+AF NQIE N    + +   ++ + EI+
Sbjct: 901  LPKTVDAIREAIAPSLAELLLSYVDEVFSYISIAFSNQIENNGVTHEPSSGINNVNLEIE 960

Query: 974  EQYNRVGGVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEI 1033
            EQYNRVGGVAVEFCVHI+R D+LFDEIFS+FV VQQRDTFLELLEPYIL+DMLGSLPPEI
Sbjct: 961  EQYNRVGGVAVEFCVHINRMDLLFDEIFSRFVAVQQRDTFLELLEPYILRDMLGSLPPEI 1020

Query: 1034 MQALVEHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRT 1093
            MQALVEHYS KGWLQR+EQCVLHMDISSLDFNQVVR+CR+HGLY AL+YLFNKGLDDFR+
Sbjct: 1021 MQALVEHYSRKGWLQRIEQCVLHMDISSLDFNQVVRICREHGLYGALLYLFNKGLDDFRS 1080

Query: 1094 PLEELLAVLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFL 1153
            PLEELL VLR S+ + A+++GY+ LVYLKYCF GLAFPPG GTL  +R  SLR EL+QFL
Sbjct: 1081 PLEELLIVLRNSEKQRATAIGYRMLVYLKYCFLGLAFPPGHGTLNPTRWPSLRSELIQFL 1140

Query: 1154 LENSDAVDTRSISNKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVD 1213
            LE S+A D  S +  +S +  LNLY LLE+DTEATLDVLR AFVE E++K  S L    +
Sbjct: 1141 LEKSNAHD--SSTCVTSRLNYLNLYHLLEMDTEATLDVLRYAFVENEMVKHESHLLEYGE 1200

Query: 1214 TSMQLQEEKNSISGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDD--WPSKKE 1273
             S++ + + +      + LIQN+VDALVHV D  +     + +GD I    D  WPSK++
Sbjct: 1201 VSVESKTDGSLPEVSNDILIQNLVDALVHVPDWGV----SNESGDPIDSKSDKNWPSKED 1260

Query: 1274 LIHLFDFIATYVACGKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREK 1333
              HLF+F+A Y A G+ ++SK V+ QIL++L S+  +P             +V S+ RE 
Sbjct: 1261 TSHLFEFVAYYAARGRVSISKSVLAQILDYLTSDHILP-----------TYNVSSKMREN 1320

Query: 1334 QVLSLLEVIPETHWNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTF 1393
            Q+L+LL+ +PET W+   V ++CEKA F+QVCG IH I  +Y +ALDSY+K+ DEPIH F
Sbjct: 1321 QLLNLLKAVPETDWDADYVSQLCEKAHFYQVCGYIHIIDRRYVAALDSYVKEADEPIHLF 1380

Query: 1394 TFINRTLLELGNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHP 1453
             ++N+ L +L   E T F++ +ISRIPEL +L+R   FFL+I +  + +  I  QL +HP
Sbjct: 1381 CYVNKMLSQLSGDEFTAFQSAIISRIPELLDLSRQGAFFLIICNLKDTIKRIQEQLHSHP 1440

Query: 1454 RSLFLYLKTLIEVHLSGSPDFSCLKKD---DNLGVNYS---TKGMDDYLQKLSDFPKYLS 1513
            RSLFLYLKT+IEV+LSGS DFS L+K    D+ G N      K    YL+ L+DFPK++ 
Sbjct: 1441 RSLFLYLKTVIEVYLSGSLDFSRLRKHEAVDSSGENIRRDIPKEAKIYLEGLNDFPKFIQ 1500

Query: 1514 NNPVDVTDDIIELYVELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLER 1573
            +NPV+VTDD+IELYVELLC++E +SVLKFLETFDSYRVEHCLRLCQ+Y ++DAAAFLLER
Sbjct: 1501 DNPVNVTDDMIELYVELLCKYEPKSVLKFLETFDSYRVEHCLRLCQEYGIVDAAAFLLER 1560

Query: 1574 VGDVGSALFLTLSSLDKKFHDLEAAVGATVSNT---ASSGSNDSQNFNSVLKLQEVNAVK 1633
            VGD GSAL LTLS L++K+ +LE AV   +S     AS G++  ++F+S L+L+EV+ ++
Sbjct: 1561 VGDAGSALSLTLSGLNEKYVELEIAVECLMSEMKLGASEGAS-LEHFSSALELKEVHDIQ 1620

Query: 1634 VLLHACIGLCQRNTPRLNSEESQTLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESS 1693
             +L ACIGLCQRNTPRLN EES+ LWF+ LD+FCEPL++SY       E      +N+ S
Sbjct: 1621 GVLQACIGLCQRNTPRLNPEESEILWFRFLDTFCEPLMESYR------EPKNTDGINKGS 1680

Query: 1694 CSQKDKEANI------VTWRILKSNKVA-HLLRKLFSQFIREIVEGMMGYVHLPTIMSRL 1753
               K  E ++      + WRI +S+  A H+LRKL SQFI+EIVEGM+GYV LPTIM++L
Sbjct: 1681 LGVKSLERHVNESDVAIKWRIPRSDTAATHILRKLISQFIKEIVEGMIGYVRLPTIMTKL 1740

Query: 1754 LYDNGSQEFGDFKLTILGMLGTFGFERRILDSAKALIEDDSFYTMSLLKKGAAHGYAPRS 1813
            L DNG+QEFGDFKLTILGMLGT+GFERRILD+AK+LIEDD+FY+M+LLKKGA+HGYAPRS
Sbjct: 1741 LSDNGTQEFGDFKLTILGMLGTYGFERRILDTAKSLIEDDTFYSMNLLKKGASHGYAPRS 1800

Query: 1814 VVCCICNRLLVKSSSSYRVRVFNCGHATHLQCEDLENEASGGDYT-------CPICVHSN 1873
            ++CCIC+  L K+ S+ RVRVFNCGHATHLQCE  ENE S    +       CP+C+   
Sbjct: 1801 LLCCICSCPLTKTFSALRVRVFNCGHATHLQCEPSENETSTSASSIHVSSSGCPVCMTKK 1860

Query: 1874 QSQGS-KSKA-PTEYSLVNKFSSRTQSSSGASVSYPQETDLLELPYTLQQIPRFEILTNL 1932
             S+ S K K+   +Y L++  SS   SS  AS  Y  E ++ +  +  QQ+ RFEILTNL
Sbjct: 1861 TSKSSLKGKSFYRDYGLISTVSSNAGSSQRAS-PYSHENEMSDHSHN-QQLSRFEILTNL 1896