Homology
BLAST of CsaV3_3G003900 vs. NCBI nr
Match:
KAE8650093.1 (hypothetical protein Csa_009608 [Cucumis sativus])
HSP 1 Score: 1643.6 bits (4255), Expect = 0.0e+00
Identity = 823/823 (100.00%), Postives = 823/823 (100.00%), Query Frame = 0
Query: 1 MFCLLLSIATLRVGSKVPSCTSRLRCDIAVPYNVLRLIAAQMLVHYSSSSWFIPLLCRFE 60
MFCLLLSIATLRVGSKVPSCTSRLRCDIAVPYNVLRLIAAQMLVHYSSSSWFIPLLCRFE
Sbjct: 1 MFCLLLSIATLRVGSKVPSCTSRLRCDIAVPYNVLRLIAAQMLVHYSSSSWFIPLLCRFE 60
Query: 61 TSTSSRAIESYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNK 120
TSTSSRAIESYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNK
Sbjct: 61 TSTSSRAIESYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNK 120
Query: 121 YINGFAATLDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAG 180
YINGFAATLDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAG
Sbjct: 121 YINGFAATLDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAG 180
Query: 181 RFGEDTIIGNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGF 240
RFGEDTIIGNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGF
Sbjct: 181 RFGEDTIIGNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGF 240
Query: 241 AMASGPLNISFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKV 300
AMASGPLNISFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKV
Sbjct: 241 AMASGPLNISFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKV 300
Query: 301 CWPATSGGGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVV 360
CWPATSGGGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVV
Sbjct: 301 CWPATSGGGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVV 360
Query: 361 VCSAGNDGPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKSFALVNLKLYTFQG 420
VCSAGNDGPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKSFALVNLKLYTFQG
Sbjct: 361 VCSAGNDGPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKSFALVNLKLYTFQG 420
Query: 421 SSISSSALAGGKFYPLINAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARV 480
SSISSSALAGGKFYPLINAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARV
Sbjct: 421 SSISSSALAGGKFYPLINAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARV 480
Query: 481 EKGFVVLQAGGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHI 540
EKGFVVLQAGGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHI
Sbjct: 481 EKGFVVLQAGGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHI 540
Query: 541 TPVQTQLGIKPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTR 600
TPVQTQLGIKPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTR
Sbjct: 541 TPVQTQLGIKPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTR 600
Query: 601 RVPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKP 660
RVPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKP
Sbjct: 601 RVPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKP 660
Query: 661 KATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFT 720
KATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFT
Sbjct: 661 KATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFT 720
Query: 721 LTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVG 780
LTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVG
Sbjct: 721 LTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVG 780
Query: 781 EEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG 824
EEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG
Sbjct: 781 EEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG 823
BLAST of CsaV3_3G003900 vs. NCBI nr
Match:
XP_031738719.1 (uncharacterized protein LOC101212014 [Cucumis sativus])
HSP 1 Score: 1540.8 bits (3988), Expect = 0.0e+00
Identity = 774/787 (98.35%), Postives = 774/787 (98.35%), Query Frame = 0
Query: 37 LIAAQMLVHYSSSSWFIPLLCRFETSTSSRAIESYIVYLGAPSVGSNPTNYDIEVATESQ 96
LIAAQMLVHYSSSSWFIPLLCRFETSTSSRAIESYIVYLGAPSVGSNPTNYDIEVATESQ
Sbjct: 22 LIAAQMLVHYSSSSWFIPLLCRFETSTSSRAIESYIVYLGAPSVGSNPTNYDIEVATESQ 81
Query: 97 YDLLGSVVGSKLAAKDAIKYSYNKYINGFAATLDEKQAKDLAKNPKVVSVFENKERKLHT 156
YDLLGSVVGSKLAAKDAIKYSYNKYINGFAATLDEKQAKDLAKNPKVVSVFENKERKLHT
Sbjct: 82 YDLLGSVVGSKLAAKDAIKYSYNKYINGFAATLDEKQAKDLAKNPKVVSVFENKERKLHT 141
Query: 157 TRSWHFLGVESDEGIPSNSIWNAGRFGEDTIIGNLDTGVWPESKSFNDAGYGPVPSRWRG 216
TRSWHFLGVESDEGIPSNSIWNAGRFGEDTIIGNLDTGVWPESKSFNDAGYGPVPSRWRG
Sbjct: 142 TRSWHFLGVESDEGIPSNSIWNAGRFGEDTIIGNLDTGVWPESKSFNDAGYGPVPSRWRG 201
Query: 217 ACEGGANFRCNRKLIGARYFNKGFAMASGPLNISFNTARDKQGHGSHTLSTAGGNFVPGA 276
ACEGGANFRCNRKLIGARYFNKGFAMASGPLNISFNTARDKQGHGSHTLSTAGGNFVPGA
Sbjct: 202 ACEGGANFRCNRKLIGARYFNKGFAMASGPLNISFNTARDKQGHGSHTLSTAGGNFVPGA 261
Query: 277 NVFGYGNGTAKGGSPKARVAAYKVCWPATSGGGCYDADILAGFEAAISDGVDVLSVSLGS 336
NVFGYGNGTAKGGSPKARVAAYKVCWPATSGGGCYDADILAGFEAAISDGVDVLSVSLGS
Sbjct: 262 NVFGYGNGTAKGGSPKARVAAYKVCWPATSGGGCYDADILAGFEAAISDGVDVLSVSLGS 321
Query: 337 KPEEFAYDSMSIGAFHAVQQGIVVVCSAGNDGPGPGTVSNISPWMFTVAASSIDRDFTSY 396
KPEEFAYDSMSIGAFHAVQQGIVVVCSAGNDGPGPGTVSNISPWMFTVAASSIDRDFTSY
Sbjct: 322 KPEEFAYDSMSIGAFHAVQQGIVVVCSAGNDGPGPGTVSNISPWMFTVAASSIDRDFTSY 381
Query: 397 ASLGNKKHYKSFALVNLKLYTFQGSSISSSALAGGKFYPLINAVDAKAANASEILAQLCH 456
ASLGNKKHYK GSSISSSALAGGKFYPLINAVDAKAANASEILAQLCH
Sbjct: 382 ASLGNKKHYK-------------GSSISSSALAGGKFYPLINAVDAKAANASEILAQLCH 441
Query: 457 KGSLDPTKAKGKIIVCLRGENARVEKGFVVLQAGGVGMILVNGKNGGSGTTADAHILPAT 516
KGSLDPTKAKGKIIVCLRGENARVEKGFVVLQAGGVGMILVNGKNGGSGTTADAHILPAT
Sbjct: 442 KGSLDPTKAKGKIIVCLRGENARVEKGFVVLQAGGVGMILVNGKNGGSGTTADAHILPAT 501
Query: 517 HLSYTDGLAVAQYINSTKTPVAHITPVQTQLGIKPSPVMADFSSRGPNPITEAMLKPDIT 576
HLSYTDGLAVAQYINSTKTPVAHITPVQTQLGIKPSPVMADFSSRGPNPITEAMLKPDIT
Sbjct: 502 HLSYTDGLAVAQYINSTKTPVAHITPVQTQLGIKPSPVMADFSSRGPNPITEAMLKPDIT 561
Query: 577 GPGMSILASVTTDVTATTFPFDTRRVPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAI 636
GPGMSILASVTTDVTATTFPFDTRRVPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAI
Sbjct: 562 GPGMSILASVTTDVTATTFPFDTRRVPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAI 621
Query: 637 KSAIMTTAKTRDNTMRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLC 696
KSAIMTTAKTRDNTMRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLC
Sbjct: 622 KSAIMTTAKTRDNTMRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLC 681
Query: 697 ARGYNSLTFKNFYNKPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYV 756
ARGYNSLTFKNFYNKPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYV
Sbjct: 682 ARGYNSLTFKNFYNKPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYV 741
Query: 757 ARVNASSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRS 816
ARVNASSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRS
Sbjct: 742 ARVNASSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRS 795
Query: 817 PIVVNLG 824
PIVVNLG
Sbjct: 802 PIVVNLG 795
BLAST of CsaV3_3G003900 vs. NCBI nr
Match:
XP_038905686.1 (subtilisin-like protease SBT5.3 [Benincasa hispida])
HSP 1 Score: 1248.8 bits (3230), Expect = 0.0e+00
Identity = 615/755 (81.46%), Postives = 673/755 (89.14%), Query Frame = 0
Query: 69 ESYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAAT 128
+SYIVYLG S G NP+ YD++ ATESQYD+L SV GSKLAAK++I YSY++YINGFAA
Sbjct: 26 KSYIVYLGLHSFGLNPSKYDVQRATESQYDILSSVTGSKLAAKESIMYSYSRYINGFAAV 85
Query: 129 LDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGEDTII 188
LDEK+A LAKNP VVSVFENKERKLHTT+SW FLGV+SD GIPSNSIW A RFGEDTII
Sbjct: 86 LDEKEAMALAKNPSVVSVFENKERKLHTTKSWSFLGVDSDAGIPSNSIWKAARFGEDTII 145
Query: 189 GNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGPLN 248
GNLDTG WPESKSFNDAGYGPVPSRWRGAC+GGANFRCNRKLIGARYFN+GFAMA+GPLN
Sbjct: 146 GNLDTGAWPESKSFNDAGYGPVPSRWRGACDGGANFRCNRKLIGARYFNQGFAMANGPLN 205
Query: 249 ISFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWPATSGG 308
+SFNTARDK+GHGSHTLSTAGGNFVPGAN+FGYGNGTAKGGSPKARVAAYKVCWPA + G
Sbjct: 206 VSFNTARDKEGHGSHTLSTAGGNFVPGANIFGYGNGTAKGGSPKARVAAYKVCWPAPT-G 265
Query: 309 GCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGNDG 368
GC+D+DILAGFEAAI DGVDVLSVSLG+ +EFAYD++SIGAFHAVQ+GIVVVCS GNDG
Sbjct: 266 GCFDSDILAGFEAAIGDGVDVLSVSLGTGAQEFAYDAVSIGAFHAVQKGIVVVCSGGNDG 325
Query: 369 PGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKSFALVNLKLYTFQGSSISSSAL 428
P PGTVSN+SPWMFTVAAS+IDRDF SY LGNKKH +GSS+SSS L
Sbjct: 326 PSPGTVSNVSPWMFTVAASTIDRDFASYVLLGNKKH-------------IRGSSLSSSGL 385
Query: 429 AGGKFYPLINAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVVLQ 488
G KFYPLINA++AKAANA++ LAQ C +GSLDP KAKGKIIVCLRGENARVEK FVVL
Sbjct: 386 RGRKFYPLINAIEAKAANATDSLAQFCEQGSLDPAKAKGKIIVCLRGENARVEKSFVVLH 445
Query: 489 AGGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQLG 548
AGGVGMI+VN + GSGT ADAHILPATH+SYTDGL+++QYI STKTPVA+IT V+T++G
Sbjct: 446 AGGVGMIMVNDQKDGSGTLADAHILPATHVSYTDGLSISQYIKSTKTPVAYITHVKTEVG 505
Query: 549 IKPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNVES 608
IKPSPVMADFSSRGPN ITEAMLKPDIT PG++I+ASVT D TAT PFDTRRVPFNVES
Sbjct: 506 IKPSPVMADFSSRGPNSITEAMLKPDITAPGVNIIASVTIDATATDSPFDTRRVPFNVES 565
Query: 609 GTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPFDYG 668
GTSMSCPHISGV GLLKTLYPTWSPAAIKSAIMTTAKTRDNT RTISD K KATPFDYG
Sbjct: 566 GTSMSCPHISGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNTQRTISDTAKMKATPFDYG 625
Query: 669 AGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFTLTDLNYPS 728
AGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNS+ K FYNKPF+CAKSF +TDLNYPS
Sbjct: 626 AGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSVALKKFYNKPFICAKSFAITDLNYPS 685
Query: 729 ISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFKVV 788
IS+P+L+ GAPVTVNRRVKNVGTPGTYVARV AS I V+VEPSTLQFNSVGEEKAFKVV
Sbjct: 686 ISVPELRIGAPVTVNRRVKNVGTPGTYVARVKASPAISVSVEPSTLQFNSVGEEKAFKVV 745
Query: 789 FEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG 824
F+YKG EQ +G+VFGTLIWSDGKH VRSPI V LG
Sbjct: 746 FQYKGKEQRQGHVFGTLIWSDGKHFVRSPIAVKLG 766
BLAST of CsaV3_3G003900 vs. NCBI nr
Match:
XP_008449184.1 (PREDICTED: subtilisin-like protease SBT5.3 [Cucumis melo])
HSP 1 Score: 1247.3 bits (3226), Expect = 0.0e+00
Identity = 625/758 (82.45%), Postives = 679/758 (89.58%), Query Frame = 0
Query: 69 ESYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAAT 128
+SYIVYLGA S G NPTN+DIE ATES YDLLGSVVG+ L AKD+IKYSYNKYINGFAA
Sbjct: 27 KSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVVGNNLEAKDSIKYSYNKYINGFAAV 86
Query: 129 LDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGEDTII 188
LDE+QA DLAKNPKVVSVFENK+R+LHTTRSW FLG+E+DEGIPSNSIW A RFGEDTII
Sbjct: 87 LDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSFLGMENDEGIPSNSIWKAARFGEDTII 146
Query: 189 GNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGPLN 248
GNLD+GVWPESKSFNDAGYGPVPSRWRGACEGG NF+CNRKLIGARYF+ GF M SG +N
Sbjct: 147 GNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNFQCNRKLIGARYFHAGFVMGSGLIN 206
Query: 249 ISFN-TARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCW-PATS 308
+SFN TARD+ GHGSHTLSTAGGNFVPGA++FGYGNGTAKGGSPKARVAAY+VCW PA
Sbjct: 207 VSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGNGTAKGGSPKARVAAYRVCWPPALF 266
Query: 309 GGGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGN 368
GGGCYDADILAGFEAAISDGVDVLSVSLGS+ +EF++DSMSIGAFHAV+QGIVVVCSAGN
Sbjct: 267 GGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFSHDSMSIGAFHAVEQGIVVVCSAGN 326
Query: 369 DGPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKSFALVNLKLYTFQGSSISSS 428
GPGP TVSN+SPWMFTV AS+IDRDFTSYA+LGNKK YK GSS+SSS
Sbjct: 327 SGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNKKQYK-------------GSSLSSS 386
Query: 429 ALAGGKFYPLINAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVV 488
+LAGGKFYPLINAVDA+ N+++ AQ C GSLDP K KGKI+VCLRG ARVEKG+VV
Sbjct: 387 SLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRGVTARVEKGYVV 446
Query: 489 LQAGGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQ 548
LQAGGVGMILVN K GSG +D+HILPAT L+YTDGLAVAQYINST TPVA ITPV+TQ
Sbjct: 447 LQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINSTTTPVALITPVETQ 506
Query: 549 LGIKPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNV 608
LG+KPSPVMA FSSRGPNPIT+AMLKPDITGPG++ILASVTTDVTAT FPFDTRRVPFNV
Sbjct: 507 LGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTATKFPFDTRRVPFNV 566
Query: 609 ESGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPFD 668
ESGTSMSCPHI+GV GLLKTLYPTWSPAAIKSAIMTTAKTRDN+M T+SD VK KATPFD
Sbjct: 567 ESGTSMSCPHIAGVSGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTLSDTVKAKATPFD 626
Query: 669 YGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFTLTDLNY 728
YGAGHV+PN+AMDPGLVYDTTIDDYLNFLCARGYN+ T +F++KPFVCA FTLTDLNY
Sbjct: 627 YGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFVCATPFTLTDLNY 686
Query: 729 PSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFK 788
PSISIP+L+ GAPVTVNRRVKNVGTPGTYVARV ASSKI VTVEPSTLQFNSVGEEKAFK
Sbjct: 687 PSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPSTLQFNSVGEEKAFK 746
Query: 789 VVFEYKGNEQDKGYVFGTLIWSD-GKHNVRSPIVVNLG 824
V+FEYKG Q K YVFGTLIWSD GKHNVRSPIVV LG
Sbjct: 747 VLFEYKGTGQGKSYVFGTLIWSDNGKHNVRSPIVVKLG 771
BLAST of CsaV3_3G003900 vs. NCBI nr
Match:
TYK19386.1 (subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa])
HSP 1 Score: 1134.0 bits (2932), Expect = 0.0e+00
Identity = 553/764 (72.38%), Postives = 643/764 (84.16%), Query Frame = 0
Query: 60 ETSTSSRAIESYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYN 119
+T R SYIVYLG+ GSNP+ +D+++ATESQY+LL SVVGSKLAAK++I YSYN
Sbjct: 733 KTPLGIRKQNSYIVYLGSHFFGSNPSIHDVQLATESQYELLESVVGSKLAAKESIFYSYN 792
Query: 120 KYINGFAATLDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNA 179
+YINGFAA LDE QA LA+NP VVS+FEN++RKLHTTRSW FLG+ESDEGIP NSIW A
Sbjct: 793 RYINGFAAILDENQAIALARNPNVVSIFENQKRKLHTTRSWSFLGMESDEGIPPNSIWKA 852
Query: 180 GRFGEDTIIGNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKG 239
RFGEDTIIGNLDTG WPESKSFNDAGYGPVPSRW G CEGGANF CN+KLIGARYFNKG
Sbjct: 853 ARFGEDTIIGNLDTGAWPESKSFNDAGYGPVPSRWMGVCEGGANFTCNKKLIGARYFNKG 912
Query: 240 FAMASGPLNISFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYK 299
F +GP++ + TARD++GHGSHTLSTAGGNFVPGANVFG GNGTAKGGSP+AR+AAYK
Sbjct: 913 FEAENGPMSANLTTARDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPRARLAAYK 972
Query: 300 VCWPATSGGGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIV 359
VCWP+ + GGCYDADILA E+AI DGVDVLS+SLGS +FA D++SIGAFHAVQQGIV
Sbjct: 973 VCWPSFT-GGCYDADILAAVESAIHDGVDVLSISLGSSARDFASDTLSIGAFHAVQQGIV 1032
Query: 360 VVCSAGNDGPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKSFALVNLKLYTFQ 419
VVCS GNDGP PGTV+N+SPWM TVAAS++DRDF +Y +LGNK+H+K
Sbjct: 1033 VVCSGGNDGPTPGTVTNVSPWMITVAASTVDRDFVNYVALGNKRHFK------------- 1092
Query: 420 GSSISSSALAGGKFYPLINAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENAR 479
G S+SS L GKFYPL++ V KA NA++ LA LC GSLDP KAKGKI++CLRG++AR
Sbjct: 1093 GVSLSSGGLPRGKFYPLVDGVQVKAGNATDKLALLCEDGSLDPAKAKGKIVLCLRGDSAR 1152
Query: 480 VEKGFVVLQAGGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAH 539
++K F V +AGG+G+ILVN K G+ TAD H LPA+HL+Y DG+A+ QYINSTK+P+A
Sbjct: 1153 MDKSFEVRRAGGIGLILVNDKEDGNDITADPHFLPASHLNYADGIAIFQYINSTKSPMAF 1212
Query: 540 ITPVQTQLGIKPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDT 599
IT V+T+LGIKPSP++ADFSSRGPNPI ++M+KPDI PG+SILA+ + TAT FP DT
Sbjct: 1213 ITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAAPGVSILAAFSEYATATDFPLDT 1272
Query: 600 RRVPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVK 659
RRV FN ESGTSM+CPHISGVVGLLKTLYP WSPAAIKSAIMTTAKTRDN+M++I D K
Sbjct: 1273 RRVSFNFESGTSMACPHISGVVGLLKTLYPKWSPAAIKSAIMTTAKTRDNSMKSILDYNK 1332
Query: 660 PKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSF 719
KATPF YGAGHVHPN+A+DPGLVYDTTI+DY+NF+CA+GYNS T K FYNKP++C KSF
Sbjct: 1333 AKATPFQYGAGHVHPNNAIDPGLVYDTTIEDYMNFICAQGYNSTTLKRFYNKPYLCPKSF 1392
Query: 720 TLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSV 779
LTDLNYPSIS+PKL G PVT+NRR+KNVGTPGTYVARV SSK+ VTV+PSTLQFNSV
Sbjct: 1393 PLTDLNYPSISVPKLTIGVPVTINRRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQFNSV 1452
Query: 780 GEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG 824
GEEKAFKVVFEYKG Q KG+VFGTLIWSDGKH VRSP+VV LG
Sbjct: 1453 GEEKAFKVVFEYKGKGQGKGHVFGTLIWSDGKHFVRSPMVVKLG 1482
BLAST of CsaV3_3G003900 vs. ExPASy Swiss-Prot
Match:
Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)
HSP 1 Score: 863.2 bits (2229), Expect = 2.3e-249
Identity = 441/762 (57.87%), Postives = 538/762 (70.60%), Query Frame = 0
Query: 64 SSRAIESYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYIN 123
+S+ SY+VY GA S T ++ E+ YD LGS GS+ A DAI YSY K+IN
Sbjct: 25 ASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHIN 84
Query: 124 GFAATLDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFG 183
GFAA LD A +++K+P+VVSVF NK KLHTTRSW FLG+E + +PS+SIW RFG
Sbjct: 85 GFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFG 144
Query: 184 EDTIIGNLDTGVWPESKSFNDAGYGPVPSRWRGACEG--GANFRCNRKLIGARYFNKGFA 243
EDTII NLDTGVWPESKSF D G GP+PSRW+G C+ A F CNRKLIGARYFNKG+A
Sbjct: 145 EDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYA 204
Query: 244 MASGPLNISFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVC 303
A G LN SF++ RD GHGSHTLSTA G+FVPG ++FG GNGTAKGGSP+ARVAAYKVC
Sbjct: 205 AAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVC 264
Query: 304 WPATSGGGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVV 363
WP G CYDAD+LA F+AAI DG DV+SVSLG +P F DS++IG+FHA ++ IVVV
Sbjct: 265 WPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVV 324
Query: 364 CSAGNDGPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKSFALVNLKLYTFQGS 423
CSAGN GP TVSN++PW TV AS++DR+F S LGN KHYK G
Sbjct: 325 CSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYK-------------GQ 384
Query: 424 SISSSALAGGKFYPLINAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVE 483
S+SS+AL KFYP++ +V+AKA NAS + AQLC GSLDP K KGKI+VCLRG+N RVE
Sbjct: 385 SLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVE 444
Query: 484 KGFVVLQAGGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHIT 543
KG V GG+GM+L N G+ AD H+LPAT L+ D AV++YI+ TK P+AHIT
Sbjct: 445 KGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHIT 504
Query: 544 PVQTQLGIKPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRR 603
P +T LG+KP+PVMA FSS+GP+ + +LKPDIT PG+S++A+ T V+ T FD RR
Sbjct: 505 PSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRR 564
Query: 604 VPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPK 663
+ FN SGTSMSCPHISG+ GLLKT YP+WSPAAI+SAIMTTA D+ I + K
Sbjct: 565 LLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMK 624
Query: 664 ATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAK-SFT 723
ATPF +GAGHV PN A++PGLVYD I DYLNFLC+ GYN+ F F C+ +
Sbjct: 625 ATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKIS 684
Query: 724 LTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVG 783
L +LNYPSI++P L + VTV+R VKNVG P Y +VN + V V+P++L F VG
Sbjct: 685 LVNLNYPSITVPNLT-SSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVG 744
Query: 784 EEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNL 823
E+K FKV+ KGYVFG L+WSD KH VRSPIVV L
Sbjct: 745 EQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772
BLAST of CsaV3_3G003900 vs. ExPASy Swiss-Prot
Match:
F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)
HSP 1 Score: 788.5 bits (2035), Expect = 7.4e-227
Identity = 413/756 (54.63%), Postives = 507/756 (67.06%), Query Frame = 0
Query: 69 ESYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAAT 128
+SYIVYLG+ + ++ ++ S L S VGS AK+AI YSY ++INGFAA
Sbjct: 40 KSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFAAI 99
Query: 129 LDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGEDTII 188
LDE +A ++AK+P VVSVF NK RKLHTT SW+F+ + + + +S+WN +GEDTII
Sbjct: 100 LDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTII 159
Query: 189 GNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASG-PL 248
NLDTGVWPESKSF+D GYG VP+RW+G C + CNRKLIGARYFNKG+ +G P
Sbjct: 160 ANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCNRKLIGARYFNKGYLAYTGLPS 219
Query: 249 NISFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWPATSG 308
N S+ T RD GHGSHTLSTA GNFVPGANVFG GNGTA GGSPKARVAAYKVCWP G
Sbjct: 220 NASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDG 279
Query: 309 GGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGND 368
C+DADILA EAAI DGVDVLS S+G ++ D ++IG+FHAV+ G+ VVCSAGN
Sbjct: 280 AECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNS 339
Query: 369 GPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKSFALVNLKLYTFQGSSISSSA 428
GP GTVSN++PW+ TV ASS+DR+F ++ L N + +F+G+S+ S
Sbjct: 340 GPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQ-------------SFKGTSL-SKP 399
Query: 429 LAGGKFYPLINAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVVL 488
L K Y LI+A DA AN + A LC KGSLDP K KGKI+VCLRG+NARV+KG
Sbjct: 400 LPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAA 459
Query: 489 QAGGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQL 548
AG GM+L N K G+ +DAH+LPA+ + Y DG + Y++STK P +I L
Sbjct: 460 AAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATL 519
Query: 549 GIKPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNVE 608
KP+P MA FSSRGPN IT +LKPDIT PG++I+A+ T T D RR PFN E
Sbjct: 520 NTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTE 579
Query: 609 SGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPFDY 668
SGTSMSCPHISGVVGLLKTL+P WSPAAI+SAIMTT++TR+N + + D KA PF Y
Sbjct: 580 SGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSY 639
Query: 669 GAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKP-FVCAKSFTLTDLNY 728
G+GHV PN A PGLVYD T DYL+FLCA GYN+ + F P + C + L D NY
Sbjct: 640 GSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNY 699
Query: 729 PSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFK 788
PSI++P L +TV R++KNVG P TY AR + V+VEP L FN GE K F+
Sbjct: 700 PSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQ 759
Query: 789 VVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNL 823
+ GYVFG L W+D H VRSPIVV L
Sbjct: 760 MTLR-PLPVTPSGYVFGELTWTDSHHYVRSPIVVQL 776
BLAST of CsaV3_3G003900 vs. ExPASy Swiss-Prot
Match:
I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)
HSP 1 Score: 726.1 bits (1873), Expect = 4.5e-208
Identity = 405/801 (50.56%), Postives = 523/801 (65.29%), Query Frame = 0
Query: 44 VHYSSSSWFIPLLCRFETSTSSRAIESYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSV 103
+H SS+F L F + + + + YIVY+GA S G +PT+ D+E+AT+S YDLLGS+
Sbjct: 8 LHLILSSFF---LFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSI 67
Query: 104 VGSKLAAKDAIKYSYNKYINGFAATLDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFL 163
GS+ AK+AI YSYN++INGFAA L+E++A D+AKNP VVSVF +KE KLHTTRSW FL
Sbjct: 68 FGSREKAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFL 127
Query: 164 GVESDEGIPSNSIWNAGRFGEDTIIGNLDTGVWPESKSFNDAGYGPVPSRWRGA-CE--- 223
G+ NS W GRFGE+TIIGN+DTGVWPES+SF+D GYG VPS+WRG C+
Sbjct: 128 GLHRR---GQNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINK 187
Query: 224 --GGANFRCNRKLIGARYFNKGFAMASGPLNISFNTARDKQGHGSHTLSTAGGNFVPGAN 283
G CNRKLIGARY+NK F +G L+ +TARD GHG+HTLSTAGGNFVPGA
Sbjct: 188 LPGSMKNTCNRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGAR 247
Query: 284 VFGYGNGTAKGGSPKARVAAYKVCWPATSGGGCYDADILAGFEAAISDGVDVLSVSLGSK 343
VF GNGTAKGGSP+ARVAAYKVCW T CY AD+LA + AI DGVDV++VS G
Sbjct: 248 VFAVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVS 307
Query: 344 ----PEEFAYDSMSIGAFHAVQQGIVVVCSAGNDGPGPGTVSNISPWMFTVAASSIDRDF 403
E D +SIGAFHA+ + I++V SAGNDGP PGTV+N++PW+FT+AAS++DRDF
Sbjct: 308 YVVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDF 367
Query: 404 TSYASLGNKKHYKSFALVNLKLYTFQGSSISSSALAGGKFYPLINAVDAKAANASEILAQ 463
+S ++ N+ +G+S+ + L + + LI + DAK ANA+ AQ
Sbjct: 368 SSNLTINNQ--------------LIEGASLFVN-LPPNQAFSLILSTDAKLANATFRDAQ 427
Query: 464 LCHKGSLDPTKAKGKIIVCLR-GENARVEKGFVVLQAGGVGMILVNGKNGGSGTTADAHI 523
LC +G+LD TK GKI++C R G+ V +G L AG GMIL N G +A+ H+
Sbjct: 428 LCRRGTLDRTKVNGKIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHV 487
Query: 524 L------PATHLSYTDGL---AVAQYINSTKT-PVAHITPVQTQLGIKPSPVMADFSSRG 583
P S G+ A+ + KT ++ +T G KP+PVMA FSSRG
Sbjct: 488 FSTVNTPPRRAKSRPHGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRG 547
Query: 584 PNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRR-VPFNVESGTSMSCPHISGVV 643
PN I ++LKPD+T PG++ILA+ + +A++ D RR FNV GTSMSCPH SG+
Sbjct: 548 PNKIQPSILKPDVTAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIA 607
Query: 644 GLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNV-KPKATPFDYGAGHVHPNSAMDP 703
GLLKT +P+WSPAAIKSAIMTTA T DNT R I D K A F YG+GHV P+ A++P
Sbjct: 608 GLLKTRHPSWSPAAIKSAIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEP 667
Query: 704 GLVYDTTIDDYLNFLCARGYNSLTFKNF-YNKPFVCAKSFTLTDLNYPSISIPKLQFGAP 763
GLVYD ++ DYLNFLCA GY+ +N+ F+C+ S ++ DLNYPSI++P L+ P
Sbjct: 668 GLVYDLSLTDYLNFLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRL-KP 727
Query: 764 VTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNEQDKG 821
VT+ R V NVG P TY + + + V P +L F +GE K FKV+ + +
Sbjct: 728 VTIARTVTNVGPPSTYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRK 786
BLAST of CsaV3_3G003900 vs. ExPASy Swiss-Prot
Match:
O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)
HSP 1 Score: 594.3 bits (1531), Expect = 2.0e-168
Identity = 342/788 (43.40%), Postives = 486/788 (61.68%), Query Frame = 0
Query: 46 YSSSSWFIPLLCR--FETSTSSRAIESYIVYLGAPSVGSNPTNYDIEVATESQYD-LLGS 105
+ SS+ F LLC S+SS +YIV++ + P+++D+ + YD L S
Sbjct: 5 FLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQM---PSSFDLH---SNWYDSSLRS 64
Query: 106 VVGSKLAAKDAIKYSYNKYINGFAATLDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHF 165
+ S + Y+Y I+GF+ L +++A L P V+SV +LHTTR+ F
Sbjct: 65 ISDSA-----ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLF 124
Query: 166 LGVESDEGIPSNSIWNAGRFGEDTIIGNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGA 225
LG++ ++ AG + D ++G LDTGVWPESKS++D G+GP+PS W+G CE G
Sbjct: 125 LGLDEH---TADLFPEAGSY-SDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGT 184
Query: 226 NFR---CNRKLIGARYFNKGFAMASGPLNIS--FNTARDKQGHGSHTLSTAGGNFVPGAN 285
NF CNRKLIGAR+F +G+ GP++ S + RD GHG+HT STA G+ V GA+
Sbjct: 185 NFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGAS 244
Query: 286 VFGYGNGTAKGGSPKARVAAYKVCWPATSGGGCYDADILAGFEAAISDGVDVLSVSLGSK 345
+ GY +GTA+G +P+ARVA YKVCW GGC+ +DILA + AI+D V+VLS+SLG
Sbjct: 245 LLGYASGTARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGG 304
Query: 346 PEEFAYDSMSIGAFHAVQQGIVVVCSAGNDGPGPGTVSNISPWMFTVAASSIDRDFTSYA 405
++ D ++IGAF A+++GI+V CSAGN GP ++SN++PW+ TV A ++DRDF + A
Sbjct: 305 MSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALA 364
Query: 406 SLGNKKHYKSFALVNLKLYTFQGSSISSSALAGGKFYPLINAVDAKAANASEILAQLCHK 465
LGN K++ +L F+G ++ K P I A A+NA+ LC
Sbjct: 365 ILGNGKNFTGVSL-------FKGEALPD------KLLPFIYA--GNASNATN--GNLCMT 424
Query: 466 GSLDPTKAKGKIIVCLRGENARVEKGFVVLQAGGVGMILVNGKNGGSGTTADAHILPATH 525
G+L P K KGKI++C RG NARV+KG VV AGGVGMIL N G ADAH+LPAT
Sbjct: 425 GTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATT 484
Query: 526 LSYTDGLAVAQYINSTKTPVAHITPVQTQLGIKPSPVMADFSSRGPNPITEAMLKPDITG 585
+ G + Y+ + P A I+ + T +G+KPSPV+A FSSRGPN IT +LKPD+
Sbjct: 485 VGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIA 544
Query: 586 PGMSILASVTTDVTATTFPFDTRRVPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAIK 645
PG++ILA+ T T D+RRV FN+ SGTSMSCPH+SG+ LLK+++P WSPAAI+
Sbjct: 545 PGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIR 604
Query: 646 SAIMTTA-KTRDNTMRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLC 705
SA+MTTA KT + + +TPFD+GAGHV P +A +PGL+YD T +DYL FLC
Sbjct: 605 SALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLC 664
Query: 706 ARGYNSLTFKNFYNKPFVC--AKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGT 765
A Y S ++ + + C +KS+++ DLNYPS ++ GA R V +VG GT
Sbjct: 665 ALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGT 724
Query: 766 YVARVNA-SSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHN 822
Y +V + ++ + ++VEP+ L F E+K++ V F ++ FG++ WSDGKH
Sbjct: 725 YSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHV 755
BLAST of CsaV3_3G003900 vs. ExPASy Swiss-Prot
Match:
O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)
HSP 1 Score: 556.6 bits (1433), Expect = 4.7e-157
Identity = 316/735 (42.99%), Postives = 448/735 (60.95%), Query Frame = 0
Query: 106 SKLAAKDAIKYSYNKYINGFAATLDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGV 165
++ A + I + Y+ +GF+A + +A +L +P V++VFE++ R+LHTTRS FLG+
Sbjct: 50 TEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGL 109
Query: 166 ESDEGIPSNSIWNAGRFGEDTIIGNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANF- 225
++ +G +W+ +G D IIG DTG+WPE +SF+D GP+P RWRG CE GA F
Sbjct: 110 QNQKG-----LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFS 169
Query: 226 --RCNRKLIGARYFNKG-FAMASGPLN--ISFNTARDKQGHGSHTLSTAGGNFVPGANVF 285
CNRK+IGAR+F KG A G +N + F + RD GHG+HT STA G A++
Sbjct: 170 PRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMS 229
Query: 286 GYGNGTAKGGSPKARVAAYKVCWPATSGGGCYDADILAGFEAAISDGVDVLSVSLGSK-- 345
GY +G AKG +PKAR+AAYKVCW GC D+DILA F+AA+ DGVDV+S+S+G
Sbjct: 230 GYASGVAKGVAPKARIAAYKVCW---KDSGCLDSDILAAFDAAVRDGVDVISISIGGGDG 289
Query: 346 -PEEFAYDSMSIGAFHAVQQGIVVVCSAGNDGPGPGTVSNISPWMFTVAASSIDRDFTSY 405
+ D ++IG++ A +GI V SAGN+GP +V+N++PW+ TV AS+IDR+F +
Sbjct: 290 ITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPAD 349
Query: 406 ASLGNKKHYKSFALVNLKLYTFQGSSISSSALAGGKFYPLINAVDAKAANASEILAQLCH 465
A LG+ + +G S+ + G+ +P++ + ++AS LC
Sbjct: 350 AILGDG-------------HRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS-----LCM 409
Query: 466 KGSLDPTKAKGKIIVCLRGENARVEKGFVVLQAGGVGMILVNGKNGGSGTTADAHILPAT 525
+ +LDP + +GKI++C RG + RV KG VV +AGGVGMIL NG + G G DAH++PA
Sbjct: 410 ENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPAC 469
Query: 526 HLSYTDGLAVAQYINSTKTPVAHITPVQTQLGIKPSPVMADFSSRGPNPITEAMLKPDIT 585
+ +G + Y +S P+A I T +GIKP+PV+A FS RGPN ++ +LKPD+
Sbjct: 470 AVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLI 529
Query: 586 GPGMSILASVTTDVTATTFPFDTRRVPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAI 645
PG++ILA+ T V T P D R+ FN+ SGTSM+CPH+SG LLK+ +P WSPA I
Sbjct: 530 APGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVI 589
Query: 646 KSAIMTTAKTRDNTMRTISDNVKPK-ATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFL 705
+SA+MTT DN+ R++ D K ATP+DYG+GH++ AM+PGLVYD T DDY+ FL
Sbjct: 590 RSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFL 649
Query: 706 CARGYNSLTFKNFYNKPFVC--AKSFTLTDLNYPSIS--IPKLQFG-APVTVNRRVKNVG 765
C+ GY T + P C + + +LNYPSI+ P + G TV R NVG
Sbjct: 650 CSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVG 709
Query: 766 -TPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNE---QDKGYVFGTLI 821
Y AR+ + + VTV+P L F S + +++ V + G VFG++
Sbjct: 710 QAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVT 758
BLAST of CsaV3_3G003900 vs. ExPASy TrEMBL
Match:
A0A0A0L601 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045060 PE=3 SV=1)
HSP 1 Score: 1478.4 bits (3826), Expect = 0.0e+00
Identity = 741/754 (98.28%), Postives = 741/754 (98.28%), Query Frame = 0
Query: 70 SYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAATL 129
SYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAATL
Sbjct: 6 SYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAATL 65
Query: 130 DEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGEDTIIG 189
DEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGEDTIIG
Sbjct: 66 DEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGEDTIIG 125
Query: 190 NLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGPLNI 249
NLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGPLNI
Sbjct: 126 NLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGPLNI 185
Query: 250 SFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWPATSGGG 309
SFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWPATSGGG
Sbjct: 186 SFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWPATSGGG 245
Query: 310 CYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGNDGP 369
CYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGNDGP
Sbjct: 246 CYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGNDGP 305
Query: 370 GPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKSFALVNLKLYTFQGSSISSSALA 429
GPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYK GSSISSSALA
Sbjct: 306 GPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYK-------------GSSISSSALA 365
Query: 430 GGKFYPLINAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVVLQA 489
GGKFYPLINAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVVLQA
Sbjct: 366 GGKFYPLINAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVVLQA 425
Query: 490 GGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQLGI 549
GGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQLGI
Sbjct: 426 GGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQLGI 485
Query: 550 KPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNVESG 609
KPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNVESG
Sbjct: 486 KPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNVESG 545
Query: 610 TSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPFDYGA 669
TSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPFDYGA
Sbjct: 546 TSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPFDYGA 605
Query: 670 GHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFTLTDLNYPSI 729
GHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFTLTDLNYPSI
Sbjct: 606 GHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFTLTDLNYPSI 665
Query: 730 SIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFKVVF 789
SIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFKVVF
Sbjct: 666 SIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFKVVF 725
Query: 790 EYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG 824
EYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG
Sbjct: 726 EYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG 746
BLAST of CsaV3_3G003900 vs. ExPASy TrEMBL
Match:
A0A1S3BLG8 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491134 PE=3 SV=1)
HSP 1 Score: 1247.3 bits (3226), Expect = 0.0e+00
Identity = 625/758 (82.45%), Postives = 679/758 (89.58%), Query Frame = 0
Query: 69 ESYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAAT 128
+SYIVYLGA S G NPTN+DIE ATES YDLLGSVVG+ L AKD+IKYSYNKYINGFAA
Sbjct: 27 KSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVVGNNLEAKDSIKYSYNKYINGFAAV 86
Query: 129 LDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGEDTII 188
LDE+QA DLAKNPKVVSVFENK+R+LHTTRSW FLG+E+DEGIPSNSIW A RFGEDTII
Sbjct: 87 LDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSFLGMENDEGIPSNSIWKAARFGEDTII 146
Query: 189 GNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGPLN 248
GNLD+GVWPESKSFNDAGYGPVPSRWRGACEGG NF+CNRKLIGARYF+ GF M SG +N
Sbjct: 147 GNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNFQCNRKLIGARYFHAGFVMGSGLIN 206
Query: 249 ISFN-TARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCW-PATS 308
+SFN TARD+ GHGSHTLSTAGGNFVPGA++FGYGNGTAKGGSPKARVAAY+VCW PA
Sbjct: 207 VSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGNGTAKGGSPKARVAAYRVCWPPALF 266
Query: 309 GGGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGN 368
GGGCYDADILAGFEAAISDGVDVLSVSLGS+ +EF++DSMSIGAFHAV+QGIVVVCSAGN
Sbjct: 267 GGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFSHDSMSIGAFHAVEQGIVVVCSAGN 326
Query: 369 DGPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKSFALVNLKLYTFQGSSISSS 428
GPGP TVSN+SPWMFTV AS+IDRDFTSYA+LGNKK YK GSS+SSS
Sbjct: 327 SGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNKKQYK-------------GSSLSSS 386
Query: 429 ALAGGKFYPLINAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVV 488
+LAGGKFYPLINAVDA+ N+++ AQ C GSLDP K KGKI+VCLRG ARVEKG+VV
Sbjct: 387 SLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRGVTARVEKGYVV 446
Query: 489 LQAGGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQ 548
LQAGGVGMILVN K GSG +D+HILPAT L+YTDGLAVAQYINST TPVA ITPV+TQ
Sbjct: 447 LQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINSTTTPVALITPVETQ 506
Query: 549 LGIKPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNV 608
LG+KPSPVMA FSSRGPNPIT+AMLKPDITGPG++ILASVTTDVTAT FPFDTRRVPFNV
Sbjct: 507 LGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTATKFPFDTRRVPFNV 566
Query: 609 ESGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPFD 668
ESGTSMSCPHI+GV GLLKTLYPTWSPAAIKSAIMTTAKTRDN+M T+SD VK KATPFD
Sbjct: 567 ESGTSMSCPHIAGVSGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTLSDTVKAKATPFD 626
Query: 669 YGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFTLTDLNY 728
YGAGHV+PN+AMDPGLVYDTTIDDYLNFLCARGYN+ T +F++KPFVCA FTLTDLNY
Sbjct: 627 YGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFVCATPFTLTDLNY 686
Query: 729 PSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFK 788
PSISIP+L+ GAPVTVNRRVKNVGTPGTYVARV ASSKI VTVEPSTLQFNSVGEEKAFK
Sbjct: 687 PSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPSTLQFNSVGEEKAFK 746
Query: 789 VVFEYKGNEQDKGYVFGTLIWSD-GKHNVRSPIVVNLG 824
V+FEYKG Q K YVFGTLIWSD GKHNVRSPIVV LG
Sbjct: 747 VLFEYKGTGQGKSYVFGTLIWSDNGKHNVRSPIVVKLG 771
BLAST of CsaV3_3G003900 vs. ExPASy TrEMBL
Match:
A0A5D3D732 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00170 PE=3 SV=1)
HSP 1 Score: 1134.0 bits (2932), Expect = 0.0e+00
Identity = 553/764 (72.38%), Postives = 643/764 (84.16%), Query Frame = 0
Query: 60 ETSTSSRAIESYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYN 119
+T R SYIVYLG+ GSNP+ +D+++ATESQY+LL SVVGSKLAAK++I YSYN
Sbjct: 733 KTPLGIRKQNSYIVYLGSHFFGSNPSIHDVQLATESQYELLESVVGSKLAAKESIFYSYN 792
Query: 120 KYINGFAATLDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNA 179
+YINGFAA LDE QA LA+NP VVS+FEN++RKLHTTRSW FLG+ESDEGIP NSIW A
Sbjct: 793 RYINGFAAILDENQAIALARNPNVVSIFENQKRKLHTTRSWSFLGMESDEGIPPNSIWKA 852
Query: 180 GRFGEDTIIGNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKG 239
RFGEDTIIGNLDTG WPESKSFNDAGYGPVPSRW G CEGGANF CN+KLIGARYFNKG
Sbjct: 853 ARFGEDTIIGNLDTGAWPESKSFNDAGYGPVPSRWMGVCEGGANFTCNKKLIGARYFNKG 912
Query: 240 FAMASGPLNISFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYK 299
F +GP++ + TARD++GHGSHTLSTAGGNFVPGANVFG GNGTAKGGSP+AR+AAYK
Sbjct: 913 FEAENGPMSANLTTARDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPRARLAAYK 972
Query: 300 VCWPATSGGGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIV 359
VCWP+ + GGCYDADILA E+AI DGVDVLS+SLGS +FA D++SIGAFHAVQQGIV
Sbjct: 973 VCWPSFT-GGCYDADILAAVESAIHDGVDVLSISLGSSARDFASDTLSIGAFHAVQQGIV 1032
Query: 360 VVCSAGNDGPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKSFALVNLKLYTFQ 419
VVCS GNDGP PGTV+N+SPWM TVAAS++DRDF +Y +LGNK+H+K
Sbjct: 1033 VVCSGGNDGPTPGTVTNVSPWMITVAASTVDRDFVNYVALGNKRHFK------------- 1092
Query: 420 GSSISSSALAGGKFYPLINAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENAR 479
G S+SS L GKFYPL++ V KA NA++ LA LC GSLDP KAKGKI++CLRG++AR
Sbjct: 1093 GVSLSSGGLPRGKFYPLVDGVQVKAGNATDKLALLCEDGSLDPAKAKGKIVLCLRGDSAR 1152
Query: 480 VEKGFVVLQAGGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAH 539
++K F V +AGG+G+ILVN K G+ TAD H LPA+HL+Y DG+A+ QYINSTK+P+A
Sbjct: 1153 MDKSFEVRRAGGIGLILVNDKEDGNDITADPHFLPASHLNYADGIAIFQYINSTKSPMAF 1212
Query: 540 ITPVQTQLGIKPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDT 599
IT V+T+LGIKPSP++ADFSSRGPNPI ++M+KPDI PG+SILA+ + TAT FP DT
Sbjct: 1213 ITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDIAAPGVSILAAFSEYATATDFPLDT 1272
Query: 600 RRVPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVK 659
RRV FN ESGTSM+CPHISGVVGLLKTLYP WSPAAIKSAIMTTAKTRDN+M++I D K
Sbjct: 1273 RRVSFNFESGTSMACPHISGVVGLLKTLYPKWSPAAIKSAIMTTAKTRDNSMKSILDYNK 1332
Query: 660 PKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSF 719
KATPF YGAGHVHPN+A+DPGLVYDTTI+DY+NF+CA+GYNS T K FYNKP++C KSF
Sbjct: 1333 AKATPFQYGAGHVHPNNAIDPGLVYDTTIEDYMNFICAQGYNSTTLKRFYNKPYLCPKSF 1392
Query: 720 TLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSV 779
LTDLNYPSIS+PKL G PVT+NRR+KNVGTPGTYVARV SSK+ VTV+PSTLQFNSV
Sbjct: 1393 PLTDLNYPSISVPKLTIGVPVTINRRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQFNSV 1452
Query: 780 GEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG 824
GEEKAFKVVFEYKG Q KG+VFGTLIWSDGKH VRSP+VV LG
Sbjct: 1453 GEEKAFKVVFEYKGKGQGKGHVFGTLIWSDGKHFVRSPMVVKLG 1482
BLAST of CsaV3_3G003900 vs. ExPASy TrEMBL
Match:
A0A6J1KUL8 (uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820 PE=3 SV=1)
HSP 1 Score: 1107.8 bits (2864), Expect = 0.0e+00
Identity = 550/756 (72.75%), Postives = 621/756 (82.14%), Query Frame = 0
Query: 70 SYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAATL 129
SYIVYLG+ S NP+ YD+++ATESQYD+LGSV GSK+AAKD+I YSYN+YINGFAA L
Sbjct: 794 SYIVYLGSHSFAPNPSVYDVQLATESQYDILGSVKGSKVAAKDSILYSYNRYINGFAAVL 853
Query: 130 DEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGEDTIIG 189
DE++A LAKNP VVSVFENKERKLHTTRSW FLGV+SD GIP NSIW A RFG DTIIG
Sbjct: 854 DEQEATALAKNPSVVSVFENKERKLHTTRSWGFLGVDSDRGIPQNSIWKAARFGADTIIG 913
Query: 190 NLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGPL-- 249
NLDTGVWPES SFNDAGYGPVPSRWRGACEGG+ FRCNRKLIGARYF +GF A GPL
Sbjct: 914 NLDTGVWPESPSFNDAGYGPVPSRWRGACEGGSKFRCNRKLIGARYFYRGFQAAEGPLTT 973
Query: 250 -NISFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWPATS 309
NISF++ARD +GHGSHTLSTAGGNFV G NVFG GNGTAKGGSP+ARV AYKVCWP +
Sbjct: 974 HNISFDSARDHEGHGSHTLSTAGGNFVHGVNVFGNGNGTAKGGSPRARVVAYKVCWP-SK 1033
Query: 310 GGGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGN 369
GGCYD+DILAG EAAISDGVDVLS S+G+ +EFA D++SIGAFHAVQ GIVVVCSAGN
Sbjct: 1034 NGGCYDSDILAGIEAAISDGVDVLSASIGTLAQEFANDAISIGAFHAVQHGIVVVCSAGN 1093
Query: 370 DGPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKSFALVNLKLYTFQGSSISSS 429
DGP PG+VSN+SPWM TV AS+IDRDF SY LGNKK F+GSS+SSS
Sbjct: 1094 DGPSPGSVSNVSPWMVTVGASTIDRDFVSYVVLGNKKR-------------FRGSSLSSS 1153
Query: 430 ALAGGKFYPLINAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVV 489
L GKFYPLI AV KAANA++ AQLC G+LDPTKAKGKIIVCLRGENARV KGF V
Sbjct: 1154 RLPAGKFYPLIKAVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRGENARVSKGFEV 1213
Query: 490 LQAGGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQ 549
+ GGVGM+LVN + GS AD HILPA+HLSY DG+++AQY++STKTPVA IT T+
Sbjct: 1214 FRVGGVGMVLVNNQMDGSALVADPHILPASHLSYADGVSIAQYLSSTKTPVASITHASTE 1273
Query: 550 LGIKPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNV 609
+GIKPSP+MA FSSRGP+ IT+A++KPDIT PG++I+ASVT D++A+ P D RRVPFN+
Sbjct: 1274 MGIKPSPLMASFSSRGPDFITQAVIKPDITAPGVNIIASVTDDISASGLPLDKRRVPFNI 1333
Query: 610 ESGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPFD 669
ESGTSMSCPHISGV GLLKTL+PTWSPAAIKSA+MTTAKTRDNT T+ D K KATPFD
Sbjct: 1334 ESGTSMSCPHISGVAGLLKTLHPTWSPAAIKSAVMTTAKTRDNTKNTLLDYTKVKATPFD 1393
Query: 670 YGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFTLTDLNY 729
YGAGHVHPN AMDPGLVYDTT+DDYLNFLC RGYNS T K F NKPFVCA +F TD NY
Sbjct: 1394 YGAGHVHPNDAMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNKPFVCANNFATTDFNY 1453
Query: 730 PSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFK 789
PSI +P+LQ G VTVNRRVKNVG+ GTYVARV I V VEPS LQF+SVGEE+ FK
Sbjct: 1454 PSILVPRLQIGGSVTVNRRVKNVGSTGTYVARVRMPKGITVMVEPSKLQFHSVGEERPFK 1513
Query: 790 VVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNL 823
++F Y + +GYVFG L+WSDGKH VRS I VNL
Sbjct: 1514 LIFHYAHKLRRQGYVFGALVWSDGKHFVRSSIAVNL 1535
BLAST of CsaV3_3G003900 vs. ExPASy TrEMBL
Match:
A0A6J1GDW2 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453052 PE=3 SV=1)
HSP 1 Score: 1105.1 bits (2857), Expect = 0.0e+00
Identity = 549/757 (72.52%), Postives = 623/757 (82.30%), Query Frame = 0
Query: 69 ESYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAAT 128
+SYIVYLG+ S G NP+ YD+++ATESQYD+LGSV GSK+AAKD+I YSYN+YINGFAA
Sbjct: 30 KSYIVYLGSHSFGPNPSIYDVQLATESQYDILGSVKGSKVAAKDSILYSYNRYINGFAAV 89
Query: 129 LDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGEDTII 188
LD+++A LAKNP VVS+FENKERKLHTTRSW FLGV+SD GIP NSIW A RFGEDTII
Sbjct: 90 LDDQEATALAKNPSVVSIFENKERKLHTTRSWSFLGVDSDRGIPRNSIWKAARFGEDTII 149
Query: 189 GNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGP-- 248
GNLDTGVWPES SFNDAGYGPVP+RWRGAC+GG+ FRCNRKLIGARYF +GF + P
Sbjct: 150 GNLDTGVWPESPSFNDAGYGPVPARWRGACDGGSKFRCNRKLIGARYFYRGFQASERPWT 209
Query: 249 -LNISFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWPAT 308
NISF++A+D +GHGSHTLSTAGGNFV G NVFG GNGTAKGGSP+ARV AYKVCWP +
Sbjct: 210 KQNISFDSAQDHEGHGSHTLSTAGGNFVHGVNVFGNGNGTAKGGSPRARVVAYKVCWP-S 269
Query: 309 SGGGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAG 368
GGCYD+DILAG EAAISDGVDVLS SLG +EFA+D++SIGAFHAVQ GIVVVCSAG
Sbjct: 270 ENGGCYDSDILAGIEAAISDGVDVLSASLGMAAQEFAHDAISIGAFHAVQHGIVVVCSAG 329
Query: 369 NDGPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKSFALVNLKLYTFQGSSISS 428
NDGP PG+VSN+SPWM TV AS+IDRDF SY +LGNKK +GSS+SS
Sbjct: 330 NDGPSPGSVSNVSPWMVTVGASTIDRDFVSYVALGNKKQ-------------LRGSSLSS 389
Query: 429 SALAGGKFYPLINAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFV 488
S L GKFYPLI AV KAANA++ AQLC G+LDPTKAKGKIIVCLRGENARV KGF
Sbjct: 390 SRLPAGKFYPLIKAVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRGENARVSKGFE 449
Query: 489 VLQAGGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQT 548
V + GG+GM+LVN + GS AD HILPA+HLS DG+++ QY++STKTPVA IT T
Sbjct: 450 VHRVGGIGMVLVNNQIDGSAIVADPHILPASHLSDADGVSITQYLSSTKTPVASITHAST 509
Query: 549 QLGIKPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFN 608
++GIKPSP+MA FSSRGP+ ITEA++KPDIT PG++I+ASVT D+TA+ PFD RRVPFN
Sbjct: 510 EMGIKPSPLMASFSSRGPDFITEAVIKPDITAPGVNIIASVTNDITASGLPFDKRRVPFN 569
Query: 609 VESGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPF 668
+ESGTSMSCPHISGV GLLKTL+PTWSP AIKSAIMTTAKTRDNT TI D K KATPF
Sbjct: 570 IESGTSMSCPHISGVAGLLKTLHPTWSPGAIKSAIMTTAKTRDNTKNTILDYTKVKATPF 629
Query: 669 DYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFTLTDLN 728
DYGAGHVHPN+AMDPGLVYDTT+DDYLNFLC RGYNS T K F NKPFVCAK+F TD N
Sbjct: 630 DYGAGHVHPNNAMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNKPFVCAKNFATTDFN 689
Query: 729 YPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAF 788
YPSI +P+LQ G VTVNRRVKNVG+ GTYVARV I V VEPSTLQF+SVGEEK F
Sbjct: 690 YPSILVPRLQIGESVTVNRRVKNVGSAGTYVARVKMPKGITVMVEPSTLQFHSVGEEKPF 749
Query: 789 KVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNL 823
K+VF Y + +GYVFG L+WSDGKH VRS I VNL
Sbjct: 750 KLVFHYAHKLRRQGYVFGALVWSDGKHFVRSSIAVNL 772
BLAST of CsaV3_3G003900 vs. TAIR 10
Match:
AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 863.2 bits (2229), Expect = 1.7e-250
Identity = 441/762 (57.87%), Postives = 538/762 (70.60%), Query Frame = 0
Query: 64 SSRAIESYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYIN 123
+S+ SY+VY GA S T ++ E+ YD LGS GS+ A DAI YSY K+IN
Sbjct: 25 ASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHIN 84
Query: 124 GFAATLDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFG 183
GFAA LD A +++K+P+VVSVF NK KLHTTRSW FLG+E + +PS+SIW RFG
Sbjct: 85 GFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFG 144
Query: 184 EDTIIGNLDTGVWPESKSFNDAGYGPVPSRWRGACEG--GANFRCNRKLIGARYFNKGFA 243
EDTII NLDTGVWPESKSF D G GP+PSRW+G C+ A F CNRKLIGARYFNKG+A
Sbjct: 145 EDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYA 204
Query: 244 MASGPLNISFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVC 303
A G LN SF++ RD GHGSHTLSTA G+FVPG ++FG GNGTAKGGSP+ARVAAYKVC
Sbjct: 205 AAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVC 264
Query: 304 WPATSGGGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVV 363
WP G CYDAD+LA F+AAI DG DV+SVSLG +P F DS++IG+FHA ++ IVVV
Sbjct: 265 WPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVV 324
Query: 364 CSAGNDGPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKSFALVNLKLYTFQGS 423
CSAGN GP TVSN++PW TV AS++DR+F S LGN KHYK G
Sbjct: 325 CSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYK-------------GQ 384
Query: 424 SISSSALAGGKFYPLINAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVE 483
S+SS+AL KFYP++ +V+AKA NAS + AQLC GSLDP K KGKI+VCLRG+N RVE
Sbjct: 385 SLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVE 444
Query: 484 KGFVVLQAGGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHIT 543
KG V GG+GM+L N G+ AD H+LPAT L+ D AV++YI+ TK P+AHIT
Sbjct: 445 KGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHIT 504
Query: 544 PVQTQLGIKPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRR 603
P +T LG+KP+PVMA FSS+GP+ + +LKPDIT PG+S++A+ T V+ T FD RR
Sbjct: 505 PSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRR 564
Query: 604 VPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPK 663
+ FN SGTSMSCPHISG+ GLLKT YP+WSPAAI+SAIMTTA D+ I + K
Sbjct: 565 LLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMK 624
Query: 664 ATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAK-SFT 723
ATPF +GAGHV PN A++PGLVYD I DYLNFLC+ GYN+ F F C+ +
Sbjct: 625 ATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKIS 684
Query: 724 LTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVG 783
L +LNYPSI++P L + VTV+R VKNVG P Y +VN + V V+P++L F VG
Sbjct: 685 LVNLNYPSITVPNLT-SSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVG 744
Query: 784 EEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNL 823
E+K FKV+ KGYVFG L+WSD KH VRSPIVV L
Sbjct: 745 EQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772
BLAST of CsaV3_3G003900 vs. TAIR 10
Match:
AT5G59810.1 (Subtilase family protein )
HSP 1 Score: 788.5 bits (2035), Expect = 5.2e-228
Identity = 413/756 (54.63%), Postives = 507/756 (67.06%), Query Frame = 0
Query: 69 ESYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAAT 128
+SYIVYLG+ + ++ ++ S L S VGS AK+AI YSY ++INGFAA
Sbjct: 40 KSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFAAI 99
Query: 129 LDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGEDTII 188
LDE +A ++AK+P VVSVF NK RKLHTT SW+F+ + + + +S+WN +GEDTII
Sbjct: 100 LDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTII 159
Query: 189 GNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASG-PL 248
NLDTGVWPESKSF+D GYG VP+RW+G C + CNRKLIGARYFNKG+ +G P
Sbjct: 160 ANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCNRKLIGARYFNKGYLAYTGLPS 219
Query: 249 NISFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWPATSG 308
N S+ T RD GHGSHTLSTA GNFVPGANVFG GNGTA GGSPKARVAAYKVCWP G
Sbjct: 220 NASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDG 279
Query: 309 GGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGND 368
C+DADILA EAAI DGVDVLS S+G ++ D ++IG+FHAV+ G+ VVCSAGN
Sbjct: 280 AECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNS 339
Query: 369 GPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKSFALVNLKLYTFQGSSISSSA 428
GP GTVSN++PW+ TV ASS+DR+F ++ L N + +F+G+S+ S
Sbjct: 340 GPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQ-------------SFKGTSL-SKP 399
Query: 429 LAGGKFYPLINAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVVL 488
L K Y LI+A DA AN + A LC KGSLDP K KGKI+VCLRG+NARV+KG
Sbjct: 400 LPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAA 459
Query: 489 QAGGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQL 548
AG GM+L N K G+ +DAH+LPA+ + Y DG + Y++STK P +I L
Sbjct: 460 AAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATL 519
Query: 549 GIKPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNVE 608
KP+P MA FSSRGPN IT +LKPDIT PG++I+A+ T T D RR PFN E
Sbjct: 520 NTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTE 579
Query: 609 SGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPFDY 668
SGTSMSCPHISGVVGLLKTL+P WSPAAI+SAIMTT++TR+N + + D KA PF Y
Sbjct: 580 SGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSY 639
Query: 669 GAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKP-FVCAKSFTLTDLNY 728
G+GHV PN A PGLVYD T DYL+FLCA GYN+ + F P + C + L D NY
Sbjct: 640 GSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNY 699
Query: 729 PSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFK 788
PSI++P L +TV R++KNVG P TY AR + V+VEP L FN GE K F+
Sbjct: 700 PSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQ 759
Query: 789 VVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNL 823
+ GYVFG L W+D H VRSPIVV L
Sbjct: 760 MTLR-PLPVTPSGYVFGELTWTDSHHYVRSPIVVQL 776
BLAST of CsaV3_3G003900 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 594.3 bits (1531), Expect = 1.4e-169
Identity = 342/788 (43.40%), Postives = 486/788 (61.68%), Query Frame = 0
Query: 46 YSSSSWFIPLLCR--FETSTSSRAIESYIVYLGAPSVGSNPTNYDIEVATESQYD-LLGS 105
+ SS+ F LLC S+SS +YIV++ + P+++D+ + YD L S
Sbjct: 5 FLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQM---PSSFDLH---SNWYDSSLRS 64
Query: 106 VVGSKLAAKDAIKYSYNKYINGFAATLDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHF 165
+ S + Y+Y I+GF+ L +++A L P V+SV +LHTTR+ F
Sbjct: 65 ISDSA-----ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLF 124
Query: 166 LGVESDEGIPSNSIWNAGRFGEDTIIGNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGA 225
LG++ ++ AG + D ++G LDTGVWPESKS++D G+GP+PS W+G CE G
Sbjct: 125 LGLDEH---TADLFPEAGSY-SDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGT 184
Query: 226 NFR---CNRKLIGARYFNKGFAMASGPLNIS--FNTARDKQGHGSHTLSTAGGNFVPGAN 285
NF CNRKLIGAR+F +G+ GP++ S + RD GHG+HT STA G+ V GA+
Sbjct: 185 NFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGAS 244
Query: 286 VFGYGNGTAKGGSPKARVAAYKVCWPATSGGGCYDADILAGFEAAISDGVDVLSVSLGSK 345
+ GY +GTA+G +P+ARVA YKVCW GGC+ +DILA + AI+D V+VLS+SLG
Sbjct: 245 LLGYASGTARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGG 304
Query: 346 PEEFAYDSMSIGAFHAVQQGIVVVCSAGNDGPGPGTVSNISPWMFTVAASSIDRDFTSYA 405
++ D ++IGAF A+++GI+V CSAGN GP ++SN++PW+ TV A ++DRDF + A
Sbjct: 305 MSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALA 364
Query: 406 SLGNKKHYKSFALVNLKLYTFQGSSISSSALAGGKFYPLINAVDAKAANASEILAQLCHK 465
LGN K++ +L F+G ++ K P I A A+NA+ LC
Sbjct: 365 ILGNGKNFTGVSL-------FKGEALPD------KLLPFIYA--GNASNATN--GNLCMT 424
Query: 466 GSLDPTKAKGKIIVCLRGENARVEKGFVVLQAGGVGMILVNGKNGGSGTTADAHILPATH 525
G+L P K KGKI++C RG NARV+KG VV AGGVGMIL N G ADAH+LPAT
Sbjct: 425 GTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATT 484
Query: 526 LSYTDGLAVAQYINSTKTPVAHITPVQTQLGIKPSPVMADFSSRGPNPITEAMLKPDITG 585
+ G + Y+ + P A I+ + T +G+KPSPV+A FSSRGPN IT +LKPD+
Sbjct: 485 VGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIA 544
Query: 586 PGMSILASVTTDVTATTFPFDTRRVPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAIK 645
PG++ILA+ T T D+RRV FN+ SGTSMSCPH+SG+ LLK+++P WSPAAI+
Sbjct: 545 PGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIR 604
Query: 646 SAIMTTA-KTRDNTMRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLC 705
SA+MTTA KT + + +TPFD+GAGHV P +A +PGL+YD T +DYL FLC
Sbjct: 605 SALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLC 664
Query: 706 ARGYNSLTFKNFYNKPFVC--AKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGT 765
A Y S ++ + + C +KS+++ DLNYPS ++ GA R V +VG GT
Sbjct: 665 ALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGT 724
Query: 766 YVARVNA-SSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHN 822
Y +V + ++ + ++VEP+ L F E+K++ V F ++ FG++ WSDGKH
Sbjct: 725 YSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHV 755
BLAST of CsaV3_3G003900 vs. TAIR 10
Match:
AT4G34980.1 (subtilisin-like serine protease 2 )
HSP 1 Score: 556.6 bits (1433), Expect = 3.3e-158
Identity = 316/735 (42.99%), Postives = 448/735 (60.95%), Query Frame = 0
Query: 106 SKLAAKDAIKYSYNKYINGFAATLDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGV 165
++ A + I + Y+ +GF+A + +A +L +P V++VFE++ R+LHTTRS FLG+
Sbjct: 50 TEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGL 109
Query: 166 ESDEGIPSNSIWNAGRFGEDTIIGNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANF- 225
++ +G +W+ +G D IIG DTG+WPE +SF+D GP+P RWRG CE GA F
Sbjct: 110 QNQKG-----LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFS 169
Query: 226 --RCNRKLIGARYFNKG-FAMASGPLN--ISFNTARDKQGHGSHTLSTAGGNFVPGANVF 285
CNRK+IGAR+F KG A G +N + F + RD GHG+HT STA G A++
Sbjct: 170 PRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMS 229
Query: 286 GYGNGTAKGGSPKARVAAYKVCWPATSGGGCYDADILAGFEAAISDGVDVLSVSLGSK-- 345
GY +G AKG +PKAR+AAYKVCW GC D+DILA F+AA+ DGVDV+S+S+G
Sbjct: 230 GYASGVAKGVAPKARIAAYKVCW---KDSGCLDSDILAAFDAAVRDGVDVISISIGGGDG 289
Query: 346 -PEEFAYDSMSIGAFHAVQQGIVVVCSAGNDGPGPGTVSNISPWMFTVAASSIDRDFTSY 405
+ D ++IG++ A +GI V SAGN+GP +V+N++PW+ TV AS+IDR+F +
Sbjct: 290 ITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPAD 349
Query: 406 ASLGNKKHYKSFALVNLKLYTFQGSSISSSALAGGKFYPLINAVDAKAANASEILAQLCH 465
A LG+ + +G S+ + G+ +P++ + ++AS LC
Sbjct: 350 AILGDG-------------HRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS-----LCM 409
Query: 466 KGSLDPTKAKGKIIVCLRGENARVEKGFVVLQAGGVGMILVNGKNGGSGTTADAHILPAT 525
+ +LDP + +GKI++C RG + RV KG VV +AGGVGMIL NG + G G DAH++PA
Sbjct: 410 ENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPAC 469
Query: 526 HLSYTDGLAVAQYINSTKTPVAHITPVQTQLGIKPSPVMADFSSRGPNPITEAMLKPDIT 585
+ +G + Y +S P+A I T +GIKP+PV+A FS RGPN ++ +LKPD+
Sbjct: 470 AVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLI 529
Query: 586 GPGMSILASVTTDVTATTFPFDTRRVPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAI 645
PG++ILA+ T V T P D R+ FN+ SGTSM+CPH+SG LLK+ +P WSPA I
Sbjct: 530 APGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVI 589
Query: 646 KSAIMTTAKTRDNTMRTISDNVKPK-ATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFL 705
+SA+MTT DN+ R++ D K ATP+DYG+GH++ AM+PGLVYD T DDY+ FL
Sbjct: 590 RSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFL 649
Query: 706 CARGYNSLTFKNFYNKPFVC--AKSFTLTDLNYPSIS--IPKLQFG-APVTVNRRVKNVG 765
C+ GY T + P C + + +LNYPSI+ P + G TV R NVG
Sbjct: 650 CSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVG 709
Query: 766 -TPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNE---QDKGYVFGTLI 821
Y AR+ + + VTV+P L F S + +++ V + G VFG++
Sbjct: 710 QAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVT 758
BLAST of CsaV3_3G003900 vs. TAIR 10
Match:
AT3G14240.1 (Subtilase family protein )
HSP 1 Score: 552.4 bits (1422), Expect = 6.3e-157
Identity = 324/784 (41.33%), Postives = 450/784 (57.40%), Query Frame = 0
Query: 71 YIVYLGAPS---VGSNPTNYDIEVATESQ-------YDLLGSVVGSKLAAKDAIKYSYNK 130
+++ L +PS SN Y + V E++ + S + S ++ +I ++Y+
Sbjct: 9 FLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHTYDT 68
Query: 131 YINGFAATLDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAG 190
+GF+A L + A L +P V+SV + R LHTTRS FLG+ S + +
Sbjct: 69 VFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTD---KAGLLEES 128
Query: 191 RFGEDTIIGNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANF---RCNRKLIGARYFN 250
FG D +IG +DTGVWPE SF+D G GPVP +W+G C +F CNRKL+GAR+F
Sbjct: 129 DFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFC 188
Query: 251 KGFAMASGPLN--ISFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARV 310
G+ +G +N F + RD GHG+HT S + G +V A+ GY +G A G +PKAR+
Sbjct: 189 GGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARL 248
Query: 311 AAYKVCWPATSGGGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQ 370
AAYKVCW GCYD+DILA F+ A++DGVDV+S+S+G + D+++IGAF A+
Sbjct: 249 AAYKVCW----NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAID 308
Query: 371 QGIVVVCSAGNDGPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKSFALVNLKL 430
+GI V SAGN GPG TV+N++PWM TV A +IDRDF + LGN K + + +
Sbjct: 309 RGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGK-----MISGVSV 368
Query: 431 YTFQGSSISSSALAGGKFYPLINAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRG 490
Y G L G+ YPL+ + LC +GSLDP KGKI++C RG
Sbjct: 369 YGGPG-------LDPGRMYPLV--YGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRG 428
Query: 491 ENARVEKGFVVLQAGGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYIN---- 550
N+R KG +V + GG+GMI+ NG G G AD H+LPAT + + G + +YI+
Sbjct: 429 INSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSK 488
Query: 551 --STKTPVAHITPVQTQLGIKPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTD 610
S+K P A I T+LGI+P+PV+A FS+RGPNP T +LKPD+ PG++ILA+
Sbjct: 489 SRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDR 548
Query: 611 VTATTFPFDTRRVPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDN 670
+ + D RR FN+ SGTSM+CPH+SG+ LLK +P WSPAAI+SA++TTA T DN
Sbjct: 549 IGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDN 608
Query: 671 TMRTISD-NVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNF 730
+ + D + ++ DYG+GHVHP AMDPGLVYD T DY+NFLC Y
Sbjct: 609 SGEPMMDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTI 668
Query: 731 YNKPFVC---AKSFTLTDLNYPSISIPKLQFGAPVTVN---RRVKNVG-TPGTYVARVNA 790
+ C ++ + +LNYPS S+ Q+G R V NVG + Y ++
Sbjct: 669 TRRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRP 728
Query: 791 SSKILVTVEPSTLQFNSVGEEKAFKV---VFEYKGNEQDKGYVFGTLIWSDGKHNVRSPI 823
VTVEP L F VG++ +F V E K + G ++WSDGK NV SP+
Sbjct: 729 PRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPL 771
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q9ZSP5 | 2.3e-249 | 57.87 | Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... | [more] |
| F4JXC5 | 7.4e-227 | 54.63 | Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... | [more] |
| I1N462 | 4.5e-208 | 50.56 | Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... | [more] |
| O65351 | 2.0e-168 | 43.40 | Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... | [more] |
| O49607 | 4.7e-157 | 42.99 | Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0L601 | 0.0e+00 | 98.28 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045060 PE=3 SV=1 | [more] |
| A0A1S3BLG8 | 0.0e+00 | 82.45 | subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491134 PE=3 SV=... | [more] |
| A0A5D3D732 | 0.0e+00 | 72.38 | Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... | [more] |
| A0A6J1KUL8 | 0.0e+00 | 72.75 | uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820... | [more] |
| A0A6J1GDW2 | 0.0e+00 | 72.52 | subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453052 PE... | [more] |
