CsaV3_2G036010 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_2G036010
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	Tocopherol O-methyltransferase
Location	chr2: 24205855 .. 24209036 (+)
RNA-Seq Expression	CsaV3_2G036010
Synteny	CsaV3_2G036010

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CGAATATTTATTAGCTTGATTAAAAAAGTTTAGTCAATTAATTTTGGATCATGGGAGCTTATGATTTATAGGTCCATTAAGTTCAATAGATGAATTTGAATTAGTTGGGTAAAAGAGGCCGCCCTCTCATGTAACCCCACATCATTTTAGACTTAAATTACTTTTCATGAAAGAAAAAAACAAACGTCTGTCCTTTCTTTCAGTATCTTCTCTCTCAGCATCTCCCTGTTTGCTTTACGTTCCCAACAAATTGGCATCTATATTATATAACCAACAACAATGAACCTCACCGCAGCAACTCCAATCCATACCTCCTCCTATCCCCCTTCCTCTCTCCATTCCAATCCCTTCAACTCCATCTCTCCCCGCCCTTCCTTTTCTTCCTTCACCCCTCGCCGCTTCTCTCCTCTCCTTGCCTCCTCCTCCGCCGCCACCACCCCGCCCTCCCTCCACATGGACTCCCATCGCCTTCACAAGGGAATCGCCGAGTTCTACGACCAATCCTCTGGCCTTTGGGAGAACGTTTGGGGCCAACATATGCACCATGGATTTTACGATCCTCATTCTTCCGTTTCCCTCTCCGATCACCGCGCTGCGCAAATCCGGATGATTGAGGAGACGCTTCGCTTCGCCGGTGTGGAGGCGGCGACTGCTGTCGTGGATGTTGGGTGTGGGATTGGGGGAAGTTCTAGGTATCTGGCCACCAAGTTTGGGGCGAAATGTAGAGGTATTACTTTGAGCCCTGTTCAAGCTAAACGGGCCCAAGAGATTTCTGCCGCTGAAGGATTAAGTGATAAGGTGTGTATGGTCTTTGAATCATTTTTGTTCTGGTTTGTTCTGGTTTGTTCTTATTTGTGCTGGTTTCTTTTTCTACATTTCTTCTTTTTGCTTTTTTTTTTTTAAGATTTTCCGTGACGCTTTTGTTTTGACGTTTACTTTGATTGGATACTAAACTTGGAAATTAGTCCTCAATCAACTAATACAACCAAAATAACGAGTACCTTCTAGATTAGTCCTTTCCAAGTTTGATTGCCCATATAATTACTATATTTCAGATTCTTTGTTACAAGTTTGATCTATTTTAGTTCTATGTGACAGTGTTTTTGAAGTAAGCTTGAGTTATCCCTAAAATGAATATGGAAAAAGTATTTGTTGGGTGGGAAACATAGGTCTAACAAGCAAAGCTAAAAATTTGAACATCTGGCCGCTCAGTCAAAAGATGTGACTAAACCAATAAAACCATATTGATGTTAAGTGAAAATGGTAGGAAAAGTCAAAAGAATCCTTTTGTCCACTTTTTTAAGTTCAACAATATGTGAAAGTGAAAGCGAGAGAACACTGGTGTTTAATGTTTTGTGATTATTGTAGAAGACTTAAACCATCATAAGTTGGTCTAGTGGTTAAAAGGGTAACATAGTTTCAATATCTAATTAAGAGGTTATGGTTCAATTCATTGTGGTCACCTATTTAGGAATTAACTTTTTATTAGTTTCTTCGACACCCAATCGTTCTGTAAGATTAGTGTAAGATTAGTCGAGTTACACGTAAGTTGATCCGAACACTTACATATATAAAATGAAAGATTTGACATGTGGGATTGACATGAGTTTGAGGAGGATTATTGGTGAGTGGTAGGTATGTTTTGAAGTGGCAGATGCTCTGAACCAACCATTCTCAGATGGGGAATTTGATCTGGTATGGTCCATGGAGAGTGGGGAGCACATGCCTGACAAATCCAAGGTGATTTTTTTTTTTTTTCTTTTTCTCTATACTCTCTCACTTTTTATTATAACTTCCCTTTGTTTTAGAAGTTAGGTGTTTGTTTGTGTACTGTGTCTTTATCTGTGCTTTGATGATATATGCTTCTTTCACACCCTACAGTTTGTCAGTGAACTAGTTAGAGTTACAGCCCCAGGTGGCACTATAATTATAGTTACATGGTGTCATAGAGACCTTGGGCCTTCTGAGAGTTCCTTGCAGCCATGGGAGTTGAAGCTTCTTCAGAAGATTTGTGATGGCTTCTATCTCCCCGCTTGGTGTTCCACTGCTGATTATGTCAAATTACTCCAATCCCACAATCTCCAGGTACTATTTATTATATAGAGCTATCACATCTATTTATTATTGATGCTCCTTCAAATTTAAACACGCTGTAAACTGAGTTGGTGCCGATAAAAAGTAGTTCACTCACAACCGTTAAAATTATAATTGAAGAAATTGAAGGAAGCCAAGGCATGTGGTGGGGAATGATGAGAGTTAATTTTGGGATGTAAGGGAAGGAACATATTGATAAAAAAAAGTTGAATATTTTATATTATATATTTAAGAATAGTATTGGAAAAGCAAAGATGATGTAAAGAAAAGGGATTTGTGTTGATAGGATATAAAGAGAGCAGATTGGTCTGAAAATGTGGCTCCTTTTTGGCCAGCAGTTATTCGCTCTGCATTCACTTGGAAAGGCTTCACATCACTCTTACGAACTGGTAAATATCTCTCACATTAATATAAATAAACAAAACAAATCAAATCTTCTTGTACAATTATTTTTAAATATAGTAAAATAAAAATTTATTTATTTTAAAGTTTGTGTATTTCTTTGCGGTTAAAAACAGATTTTTTTATGACTTGAAAAGCAAAGTTGATTGAGTTGGTTTATATTATTTGTTGTTACAATTAGGATGGAAAACAATAAGAGGAGCACTAGTAATGCCATTGATGATTGAAGGTTTCAACAAAGATCTCATCAAATTTGCCATTATTACTTGCCGTAAGCCACAATGAAGAAGAAGCTAATTCTTCATAGACTTCCATAACCAAAACCAATCACTATAAAACCTCACAATCAAATTATTCCCTCCTAGTAATTCTTCTCCCTTTTATTTTCTTTTTATGTTGCATTTCTTGCCTTACTACTTATTTTGTAACATTTTTTTCACGAGTTACTTCCTTTCTTTATATTAGAAATGGGTTAGATTTTTTCCTCATCCTTCAACTTGTTTTTCTTTATGTATATCAATTGGTGTTAATAGGTGAAACATTATTCACATTATCCTTTGTAATTGCTAGAGATAATTTGTATATTTAGTGAAGAAGCTTACGTTAATTCCTATTGAATTATGTATTCGCTTTTTATAATCTAAACAAAATAAAATTGATTAAAGGTTACGACAAAAGTAAAAACA

mRNA sequence

ATGAACCTCACCGCAGCAACTCCAATCCATACCTCCTCCTATCCCCCTTCCTCTCTCCATTCCAATCCCTTCAACTCCATCTCTCCCCGCCCTTCCTTTTCTTCCTTCACCCCTCGCCGCTTCTCTCCTCTCCTTGCCTCCTCCTCCGCCGCCACCACCCCGCCCTCCCTCCACATGGACTCCCATCGCCTTCACAAGGGAATCGCCGAGTTCTACGACCAATCCTCTGGCCTTTGGGAGAACGTTTGGGGCCAACATATGCACCATGGATTTTACGATCCTCATTCTTCCGTTTCCCTCTCCGATCACCGCGCTGCGCAAATCCGGATGATTGAGGAGACGCTTCGCTTCGCCGGTGTGGAGGCGGCGACTGCTGTCGTGGATGTTGGGTGTGGGATTGGGGGAAGTTCTAGGTATCTGGCCACCAAGTTTGGGGCGAAATGTAGAGGTATTACTTTGAGCCCTGTTCAAGCTAAACGGGCCCAAGAGATTTCTGCCGCTGAAGGATTAAGTGATAAGGTATGTTTTGAAGTGGCAGATGCTCTGAACCAACCATTCTCAGATGGGGAATTTGATCTGGTATGGTCCATGGAGAGTGGGGAGCACATGCCTGACAAATCCAAGTTTGTCAGTGAACTAGTTAGAGTTACAGCCCCAGGTGGCACTATAATTATAGTTACATGGTGTCATAGAGACCTTGGGCCTTCTGAGAGTTCCTTGCAGCCATGGGAGTTGAAGCTTCTTCAGAAGATTTGTGATGGCTTCTATCTCCCCGCTTGGTGTTCCACTGCTGATTATGTCAAATTACTCCAATCCCACAATCTCCAGGATATAAAGAGAGCAGATTGGTCTGAAAATGTGGCTCCTTTTTGGCCAGCAGTTATTCGCTCTGCATTCACTTGGAAAGGCTTCACATCACTCTTACGAACTGGATGGAAAACAATAAGAGGAGCACTAGTAATGCCATTGATGATTGAAGGTTTCAACAAAGATCTCATCAAATTTGCCATTATTACTTGCCGTAAGCCACAATGA

Coding sequence (CDS)

ATGAACCTCACCGCAGCAACTCCAATCCATACCTCCTCCTATCCCCCTTCCTCTCTCCATTCCAATCCCTTCAACTCCATCTCTCCCCGCCCTTCCTTTTCTTCCTTCACCCCTCGCCGCTTCTCTCCTCTCCTTGCCTCCTCCTCCGCCGCCACCACCCCGCCCTCCCTCCACATGGACTCCCATCGCCTTCACAAGGGAATCGCCGAGTTCTACGACCAATCCTCTGGCCTTTGGGAGAACGTTTGGGGCCAACATATGCACCATGGATTTTACGATCCTCATTCTTCCGTTTCCCTCTCCGATCACCGCGCTGCGCAAATCCGGATGATTGAGGAGACGCTTCGCTTCGCCGGTGTGGAGGCGGCGACTGCTGTCGTGGATGTTGGGTGTGGGATTGGGGGAAGTTCTAGGTATCTGGCCACCAAGTTTGGGGCGAAATGTAGAGGTATTACTTTGAGCCCTGTTCAAGCTAAACGGGCCCAAGAGATTTCTGCCGCTGAAGGATTAAGTGATAAGGTATGTTTTGAAGTGGCAGATGCTCTGAACCAACCATTCTCAGATGGGGAATTTGATCTGGTATGGTCCATGGAGAGTGGGGAGCACATGCCTGACAAATCCAAGTTTGTCAGTGAACTAGTTAGAGTTACAGCCCCAGGTGGCACTATAATTATAGTTACATGGTGTCATAGAGACCTTGGGCCTTCTGAGAGTTCCTTGCAGCCATGGGAGTTGAAGCTTCTTCAGAAGATTTGTGATGGCTTCTATCTCCCCGCTTGGTGTTCCACTGCTGATTATGTCAAATTACTCCAATCCCACAATCTCCAGGATATAAAGAGAGCAGATTGGTCTGAAAATGTGGCTCCTTTTTGGCCAGCAGTTATTCGCTCTGCATTCACTTGGAAAGGCTTCACATCACTCTTACGAACTGGATGGAAAACAATAAGAGGAGCACTAGTAATGCCATTGATGATTGAAGGTTTCAACAAAGATCTCATCAAATTTGCCATTATTACTTGCCGTAAGCCACAATGA

Protein sequence

MNLTAATPIHTSSYPPSSLHSNPFNSISPRPSFSSFTPRRFSPLLASSSAATTPPSLHMDSHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETLRFAGVEAATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVCFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGPSESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVIRSAFTWKGFTSLLRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRKPQ*

Homology

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BLAST of CsaV3_2G036010 vs. NCBI nr
Match: XP_004151846.2 (tocopherol O-methyltransferase, chloroplastic isoform X1 [Cucumis sativus] >KGN63361.1 hypothetical protein Csa_021911 [Cucumis sativus])

HSP 1 Score: 709.5 bits (1830), Expect = 1.4e-200
Identity = 344/344 (100.00%), Postives = 344/344 (100.00%), Query Frame = 0


Query: 1   MNLTAATPIHTSSYPPSSLHSNPFNSISPRPSFSSFTPRRFSPLLASSSAATTPPSLHMD 60
           MNLTAATPIHTSSYPPSSLHSNPFNSISPRPSFSSFTPRRFSPLLASSSAATTPPSLHMD
Sbjct: 1   MNLTAATPIHTSSYPPSSLHSNPFNSISPRPSFSSFTPRRFSPLLASSSAATTPPSLHMD 60

Query: 61  SHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETLRFAGV 120
           SHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETLRFAGV
Sbjct: 61  SHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETLRFAGV 120

Query: 121 EAATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVCFEVAD 180
           EAATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVCFEVAD
Sbjct: 121 EAATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVCFEVAD 180

Query: 181 ALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGPSESSL 240
           ALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGPSESSL
Sbjct: 181 ALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGPSESSL 240

Query: 241 QPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVIRSAFT 300
           QPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVIRSAFT
Sbjct: 241 QPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVIRSAFT 300

Query: 301 WKGFTSLLRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRKPQ 345
           WKGFTSLLRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRKPQ
Sbjct: 301 WKGFTSLLRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRKPQ 344

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BLAST of CsaV3_2G036010 vs. NCBI nr
Match: XP_008462787.1 (PREDICTED: tocopherol O-methyltransferase, chloroplastic [Cucumis melo] >TYK00989.1 tocopherol O-methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 629.8 bits (1623), Expect = 1.4e-176
Identity = 314/349 (89.97%), Postives = 318/349 (91.12%), Query Frame = 0


Query: 1   MNLTAATPIHTSSYPPSSLHSNP-----FNSISPRPSFSSFTPRRFSPLLASSSAATTPP 60
           MN TAATPIHTS YPPSSLH N       N IS RPSFSSFTPRR SP LA    A T  
Sbjct: 1   MNFTAATPIHTSFYPPSSLHFNRLNPTILNPISLRPSFSSFTPRRSSPFLA---FAATTA 60

Query: 61  SLHMDSHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETL 120
           S HMDSHRL KGIAEFYDQSSGLWENVWG+HMHHGFYDP SSVSLSDHRAAQ+RMIEETL
Sbjct: 61  SDHMDSHRLQKGIAEFYDQSSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQVRMIEETL 120

Query: 121 RFAGVEAATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVC 180
           RFAGVE ATAVVDVGCGIGGSSRYLA KFGAKCRGITLSPVQAKRAQ I+AAEGLSDKV 
Sbjct: 121 RFAGVEEATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVY 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRV APG TIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240

Query: 241 SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVI 300
           SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWS NVAPFWPAVI
Sbjct: 241 SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSGNVAPFWPAVI 300

Query: 301 RSAFTWKGFTSLLRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRKPQ 345
           RSAFTWKGFTSLLRTGWKTI+GALVMPLMIEGFNKDLIKFAIITCRKPQ
Sbjct: 301 RSAFTWKGFTSLLRTGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKPQ 346

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BLAST of CsaV3_2G036010 vs. NCBI nr
Match: XP_038900464.1 (tocopherol O-methyltransferase, chloroplastic [Benincasa hispida])

HSP 1 Score: 605.5 bits (1560), Expect = 2.8e-169
Identity = 302/349 (86.53%), Postives = 313/349 (89.68%), Query Frame = 0


Query: 1   MNLTAATPIHTSSYPPSSLHSNPFNS--ISPRP---SFSSFTPRRFSPLLASSSAATTPP 60
           M+ T A PIHT SY PSS HSNPF    +SP P   SFSSFT RR SPLLA  +AA T  
Sbjct: 1   MSFTTAPPIHTLSYSPSSFHSNPFKPTILSPIPLRRSFSSFTHRRTSPLLA--AAAATTA 60

Query: 61  SLHMDSHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETL 120
           S  MD+ RL KGIAEFYD+SSGLWE+VWG+HMHHGFYDP SSVSL+DHRAAQIRMIEETL
Sbjct: 61  SDQMDADRLQKGIAEFYDESSGLWESVWGEHMHHGFYDPDSSVSLADHRAAQIRMIEETL 120

Query: 121 RFAGVEAATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVC 180
           RFA VE ATA VDVGCGIGGSSRYLA KFGAKCRGITLSPVQAKRAQ I+AAEGLSDKVC
Sbjct: 121 RFASVEKATAAVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVC 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRV APGGTIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGGTIIIVTWCHRDLGP 240

Query: 241 SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVI 300
           SE SLQPWE KLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIK ADWSENVAPFWPAVI
Sbjct: 241 SEKSLQPWEEKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKTADWSENVAPFWPAVI 300

Query: 301 RSAFTWKGFTSLLRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRKPQ 345
           RSA TWKGFTSLLR+GWKTI+GALVMPLMIEGFNKDLIKFAIITCRKPQ
Sbjct: 301 RSALTWKGFTSLLRSGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKPQ 347

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BLAST of CsaV3_2G036010 vs. NCBI nr
Match: KAG7011011.1 (Tocopherol O-methyltransferase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 581.6 bits (1498), Expect = 4.4e-162
Identity = 289/349 (82.81%), Postives = 307/349 (87.97%), Query Frame = 0


Query: 1   MNLTAATPIHTSSYPPSSLHSNPFNS--ISPRP---SFSSFTPRRFSPLLASSSAATTPP 60
           M+ T ATPI T SY  SSLHSN FN+  +SP P   SFSSF  RR +  L +++A TT  
Sbjct: 1   MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60

Query: 61  SLHMDSHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETL 120
            + MD   L KGIAEFYD+SSGLWENVWG+HMHHGFYDP SSVSLSDHRAAQIRMIEETL
Sbjct: 61  EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL 120

Query: 121 RFAGVEAATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVC 180
           RFAGVEAATAVVDVGCGIGGSSRYLA KFGAKCRGITLSPVQAKRAQ I+A  GLSDKVC
Sbjct: 121 RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRV APG TIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240

Query: 241 SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVI 300
           SE SLQPWE KLLQKICDGFYLPAWCSTADYVKLL+SH LQD++ ADWS NVAPFWPAVI
Sbjct: 241 SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI 300

Query: 301 RSAFTWKGFTSLLRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRKPQ 345
           RSAFTW GF+SLLR+GWKTI+GALVMPLMIEGF KDLIKFAIITCRKP+
Sbjct: 301 RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 349

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BLAST of CsaV3_2G036010 vs. NCBI nr
Match: XP_022944690.1 (tocopherol O-methyltransferase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 581.6 bits (1498), Expect = 4.4e-162
Identity = 289/349 (82.81%), Postives = 307/349 (87.97%), Query Frame = 0


Query: 1   MNLTAATPIHTSSYPPSSLHSNPFNS--ISPRP---SFSSFTPRRFSPLLASSSAATTPP 60
           M+ T ATPI T SY  SSLHSN FN+  +SP P   SFSSF  RR +  L +++A TT  
Sbjct: 1   MSFTTATPILTPSYSLSSLHSNRFNAAILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60

Query: 61  SLHMDSHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETL 120
            + MD   L KGIAEFYD+SSGLWENVWG+HMHHGFYDP SSVSLSDHRAAQIRMIEETL
Sbjct: 61  EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL 120

Query: 121 RFAGVEAATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVC 180
           RFAGVEAATAVVDVGCGIGGSSRYLA KFGAKCRGITLSPVQAKRAQ I+A  GLSDKVC
Sbjct: 121 RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRV APG TIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240

Query: 241 SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVI 300
           SE SLQPWE KLLQKICDGFYLPAWCSTADYVKLL+SH LQD++ ADWS NVAPFWPAVI
Sbjct: 241 SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI 300

Query: 301 RSAFTWKGFTSLLRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRKPQ 345
           RSAFTW GF+SLLR+GWKTI+GALVMPLMIEGF KDLIKFAIITCRKP+
Sbjct: 301 RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 349

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BLAST of CsaV3_2G036010 vs. ExPASy Swiss-Prot
Match: Q9ZSK1 (Tocopherol O-methyltransferase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=VTE4 PE=1 SV=2)

HSP 1 Score: 448.4 bits (1152), Expect = 7.5e-125
Identity = 228/342 (66.67%), Postives = 263/342 (76.90%), Query Frame = 0


Query: 16  PSSLHSNPFNSISPRPSFSSFTPRRFSPLLASSSAATTP------PSLHMDSHRLHKGIA 75
           PSSL S P+ + S   S SS   R  SP  +SS + TT        +    +  L KGIA
Sbjct: 8   PSSLTSLPYRTNSSFGSKSSLLFR--SPSSSSSVSMTTTRGNVAVAAAATSTEALRKGIA 67

Query: 76  EFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSD--HRAAQIRMIEETLRFAGV------E 135
           EFY+++SGLWE +WG HMHHGFYDP SSV LSD  H+ AQIRMIEE+LRFAGV      +
Sbjct: 68  EFYNETSGLWEEIWGDHMHHGFYDPDSSVQLSDSGHKEAQIRMIEESLRFAGVTDEEEEK 127

Query: 136 AATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVCFEVADA 195
               VVDVGCGIGGSSRYLA+KFGA+C GITLSPVQAKRA +++AA+ L+ K  F+VADA
Sbjct: 128 KIKKVVDVGCGIGGSSRYLASKFGAECIGITLSPVQAKRANDLAAAQSLAHKASFQVADA 187

Query: 196 LNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGPSESSLQ 255
           L+QPF DG+FDLVWSMESGEHMPDK+KFV ELVRV APGG IIIVTWCHR+L   E +LQ
Sbjct: 188 LDQPFEDGKFDLVWSMESGEHMPDKAKFVKELVRVAAPGGRIIIVTWCHRNLSAGEEALQ 247

Query: 256 PWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVIRSAFTW 315
           PWE  +L KIC  FYLPAWCST DYV LLQSH+LQDIK ADWSENVAPFWPAVIR+A TW
Sbjct: 248 PWEQNILDKICKTFYLPAWCSTDDYVNLLQSHSLQDIKCADWSENVAPFWPAVIRTALTW 307

Query: 316 KGFTSLLRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRKP 344
           KG  SLLR+G K+I+GAL MPLMIEG+ K +IKF IITC+KP
Sbjct: 308 KGLVSLLRSGMKSIKGALTMPLMIEGYKKGVIKFGIITCQKP 347

[Close]

BLAST of CsaV3_2G036010 vs. ExPASy Swiss-Prot
Match: Q6ZIK0 (Probable tocopherol O-methyltransferase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=VTE4 PE=2 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 5.4e-123
Identity = 212/312 (67.95%), Postives = 250/312 (80.13%), Query Frame = 0


Query: 39  RRFSPLLASSS-AATTPPSLHMDSHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSS 98
           R  +P L+SSS AA  PP        L +GIA  YD+SSG+WE++WG+HMHHGFYD   +
Sbjct: 55  RAMAPTLSSSSTAAAAPPG-------LKEGIAGLYDESSGVWESIWGEHMHHGFYDAGEA 114

Query: 99  VSLSDHRAAQIRMIEETLRFAGV-----EAATAVVDVGCGIGGSSRYLATKFGAKCRGIT 158
            S+SDHR AQIRMIEE+L FA V     +   +VVDVGCGIGGSSRYLA K+GA+C GIT
Sbjct: 115 ASMSDHRRAQIRMIEESLAFAAVPDDAEKKPKSVVDVGCGIGGSSRYLANKYGAQCYGIT 174

Query: 159 LSPVQAKRAQEISAAEGLSDKVCFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSE 218
           LSPVQA+R   ++A +GLSDKV F+V DAL QPF DG+FDLVWSMESGEHMPDK +FVSE
Sbjct: 175 LSPVQAERGNALAAEQGLSDKVSFQVGDALEQPFPDGQFDLVWSMESGEHMPDKRQFVSE 234

Query: 219 LVRVTAPGGTIIIVTWCHRDLGPSESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQS 278
           L RV APG  IIIVTWCHR+L PSE SL+P EL LL++ICD +YLP WCS +DYVK+ +S
Sbjct: 235 LARVAAPGARIIIVTWCHRNLEPSEESLKPDELNLLKRICDAYYLPDWCSPSDYVKIAES 294

Query: 279 HNLQDIKRADWSENVAPFWPAVIRSAFTWKGFTSLLRTGWKTIRGALVMPLMIEGFNKDL 338
            +L+DI+ ADWSENVAPFWPAVI+SA TWKG TSLLR+GWKTIRGA+VMPLMIEG+ K L
Sbjct: 295 LSLEDIRTADWSENVAPFWPAVIKSALTWKGLTSLLRSGWKTIRGAMVMPLMIEGYKKGL 354

Query: 339 IKFAIITCRKPQ 345
           IKF IITCRKP+
Sbjct: 355 IKFTIITCRKPE 359

[Close]

BLAST of CsaV3_2G036010 vs. ExPASy Swiss-Prot
Match: W5U2K2 (3-hydroxy-16-methoxy-2,3-dihydrotabersonine N-methyltransferase OS=Catharanthus roseus OX=4058 GN=NMT PE=1 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 1.2e-69
Identity = 127/281 (45.20%), Postives = 193/281 (68.68%), Query Frame = 0


Query: 68  IAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETLRFAGVEAATA-- 127
           +AEFYD+ +G W+  +G H+H G+Y+P ++ +++  + A IRMI+E LRFAGV    A  
Sbjct: 8   VAEFYDKVTGAWDLFYGVHLHDGYYEPGTTATMAISQDAVIRMIDELLRFAGVSEDPAKK 67

Query: 128 ---VVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVCFEVADAL 187
              ++DVG G+GG+  Y+A K+  +C GIT+SP Q K AQ+ +A EG+ +KV F+  DAL
Sbjct: 68  PRSMLDVGSGLGGTCVYVAKKYDIQCTGITISPNQVKYAQDYAATEGVENKVSFDCGDAL 127

Query: 188 NQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGPSESSLQP 247
           + P+SDG+FD+V+++   EH+ DK KF+ E+VRV APG  III +  H +L P E SL+P
Sbjct: 128 DMPYSDGKFDVVFTINCIEHVHDKEKFIREMVRVAAPGAAIIIASQAHPNLSPGE-SLKP 187

Query: 248 WELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVIRSAFTWK 307
            + K+LQKICDG    + CS+ DYV+ L    +++IK ADW++N+ P +P +++ AFTWK
Sbjct: 188 RDKKILQKICDGAGAVSLCSSDDYVRWLTPLPVKEIKAADWTQNITPLYPLLMKEAFTWK 247

Query: 308 GFTSL-LRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRK 343
           GFTS+ L+ GW+ I     + L+ +  N  ++KFA++T RK
Sbjct: 248 GFTSIVLKGGWRAINLINAVRLVAKAANDGILKFAVVTGRK 287

[Close]

BLAST of CsaV3_2G036010 vs. ExPASy Swiss-Prot
Match: P74388 (2-methyl-6-phytyl-1,4-hydroquinone methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll0418 PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 2.0e-37
Identity = 99/287 (34.49%), Postives = 148/287 (51.57%), Query Frame = 0


Query: 68  IAEFYDQ--SSGLWENVWGQHMHHGFY-DPHSSVSLSDHRAAQIRMIEETLRFAGVEA-- 127
           +A  YDQ    G+ E  WG H+H G Y DP  +    D   ++I  +    ++ G++   
Sbjct: 36  VANAYDQWTEDGILEYYWGDHIHLGHYGDPPVA---KDFIQSKIDFVHAMAQWGGLDTLP 95

Query: 128 -ATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVCFEVADA 187
             T V+DVGCGIGGSSR LA  +G    GIT+SP Q KRA E++  +  +    F V DA
Sbjct: 96  PGTTVLDVGCGIGGSSRILAKDYGFNVTGITISPQQVKRATELTPPDVTAK---FAVDDA 155

Query: 188 LNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGPSESSLQ 247
           +   F DG FD+VWS+E+G HMPDK+ F  EL+RV  PGG +++  W  RD    +  L 
Sbjct: 156 MALSFPDGSFDVVWSVEAGPHMPDKAVFAKELLRVVKPGGILVVADWNQRD--DRQVPLN 215

Query: 248 PWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQD--IKRADWSENVAPFWPAVIRSAF 307
            WE  +++++ D +  PA+ S   + + L++  L +  +  ADW+    P W   I    
Sbjct: 216 FWEKPVMRQLLDQWSHPAFASIEGFAENLEATGLVEGQVTTADWTVPTLPAWLDTI---- 275

Query: 308 TWKGFTSLLRTGW---------KTIRGALVMPLMIEGFNKDLIKFAI 338
            W+G   +   GW         K++R    + LM   F   L +F +
Sbjct: 276 -WQGI--IRPQGWLQYGIRGFIKSVREVPTILLMRLAFGVGLCRFGM 307

[Close]

BLAST of CsaV3_2G036010 vs. ExPASy Swiss-Prot
Match: Q8KZ94 (Demethylrebeccamycin-D-glucose O-methyltransferase OS=Lentzea aerocolonigenes OX=68170 GN=rebM PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 5.2e-25
Identity = 59/159 (37.11%), Postives = 94/159 (59.12%), Query Frame = 0


Query: 68  IAEFYDQSSGLWENVWGQHMHHGFY-DPHSSVSLSDHRAAQIRMIEETLRFAGVEAATAV 127
           + + YD  +  +  +WG+++H G++ D  + VS+ D   A  R+ +E +    V +   V
Sbjct: 19  VRQMYDDFTDPFARIWGENLHFGYWEDAGADVSVDD---ATDRLTDEMIALLDVRSGDRV 78

Query: 128 VDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVCFEVADALNQPF 187
           +DVGCGIG  +  LAT    +  GI++S  Q  +A   + A GL+++V F  ADA++ PF
Sbjct: 79  LDVGCGIGKPAVRLATARDVRVTGISISRPQVNQANARATAAGLANRVTFSYADAMDLPF 138

Query: 188 SDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIII 226
            D  FD VW++ES  HMPD+ + + E+ RV  PGGT+ I
Sbjct: 139 EDASFDAVWALESLHHMPDRGRALREMARVLRPGGTVAI 174

[Close]

BLAST of CsaV3_2G036010 vs. ExPASy TrEMBL
Match: A0A0A0LTM1 (Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G432780 PE=3 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 6.7e-201
Identity = 344/344 (100.00%), Postives = 344/344 (100.00%), Query Frame = 0


Query: 1   MNLTAATPIHTSSYPPSSLHSNPFNSISPRPSFSSFTPRRFSPLLASSSAATTPPSLHMD 60
           MNLTAATPIHTSSYPPSSLHSNPFNSISPRPSFSSFTPRRFSPLLASSSAATTPPSLHMD
Sbjct: 1   MNLTAATPIHTSSYPPSSLHSNPFNSISPRPSFSSFTPRRFSPLLASSSAATTPPSLHMD 60

Query: 61  SHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETLRFAGV 120
           SHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETLRFAGV
Sbjct: 61  SHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETLRFAGV 120

Query: 121 EAATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVCFEVAD 180
           EAATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVCFEVAD
Sbjct: 121 EAATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVCFEVAD 180

Query: 181 ALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGPSESSL 240
           ALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGPSESSL
Sbjct: 181 ALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGPSESSL 240

Query: 241 QPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVIRSAFT 300
           QPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVIRSAFT
Sbjct: 241 QPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVIRSAFT 300

Query: 301 WKGFTSLLRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRKPQ 345
           WKGFTSLLRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRKPQ
Sbjct: 301 WKGFTSLLRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRKPQ 344

[Close]

BLAST of CsaV3_2G036010 vs. ExPASy TrEMBL
Match: A0A5D3BSC2 (Tocopherol O-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G001840 PE=3 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 6.8e-177
Identity = 314/349 (89.97%), Postives = 318/349 (91.12%), Query Frame = 0


Query: 1   MNLTAATPIHTSSYPPSSLHSNP-----FNSISPRPSFSSFTPRRFSPLLASSSAATTPP 60
           MN TAATPIHTS YPPSSLH N       N IS RPSFSSFTPRR SP LA    A T  
Sbjct: 1   MNFTAATPIHTSFYPPSSLHFNRLNPTILNPISLRPSFSSFTPRRSSPFLA---FAATTA 60

Query: 61  SLHMDSHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETL 120
           S HMDSHRL KGIAEFYDQSSGLWENVWG+HMHHGFYDP SSVSLSDHRAAQ+RMIEETL
Sbjct: 61  SDHMDSHRLQKGIAEFYDQSSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQVRMIEETL 120

Query: 121 RFAGVEAATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVC 180
           RFAGVE ATAVVDVGCGIGGSSRYLA KFGAKCRGITLSPVQAKRAQ I+AAEGLSDKV 
Sbjct: 121 RFAGVEEATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVY 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRV APG TIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240

Query: 241 SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVI 300
           SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWS NVAPFWPAVI
Sbjct: 241 SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSGNVAPFWPAVI 300

Query: 301 RSAFTWKGFTSLLRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRKPQ 345
           RSAFTWKGFTSLLRTGWKTI+GALVMPLMIEGFNKDLIKFAIITCRKPQ
Sbjct: 301 RSAFTWKGFTSLLRTGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKPQ 346

[Close]

BLAST of CsaV3_2G036010 vs. ExPASy TrEMBL
Match: A0A1S3CHR0 (tocopherol O-methyltransferase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501078 PE=3 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 6.8e-177
Identity = 314/349 (89.97%), Postives = 318/349 (91.12%), Query Frame = 0


Query: 1   MNLTAATPIHTSSYPPSSLHSNP-----FNSISPRPSFSSFTPRRFSPLLASSSAATTPP 60
           MN TAATPIHTS YPPSSLH N       N IS RPSFSSFTPRR SP LA    A T  
Sbjct: 1   MNFTAATPIHTSFYPPSSLHFNRLNPTILNPISLRPSFSSFTPRRSSPFLA---FAATTA 60

Query: 61  SLHMDSHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETL 120
           S HMDSHRL KGIAEFYDQSSGLWENVWG+HMHHGFYDP SSVSLSDHRAAQ+RMIEETL
Sbjct: 61  SDHMDSHRLQKGIAEFYDQSSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQVRMIEETL 120

Query: 121 RFAGVEAATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVC 180
           RFAGVE ATAVVDVGCGIGGSSRYLA KFGAKCRGITLSPVQAKRAQ I+AAEGLSDKV 
Sbjct: 121 RFAGVEEATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVY 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRV APG TIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240

Query: 241 SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVI 300
           SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWS NVAPFWPAVI
Sbjct: 241 SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSGNVAPFWPAVI 300

Query: 301 RSAFTWKGFTSLLRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRKPQ 345
           RSAFTWKGFTSLLRTGWKTI+GALVMPLMIEGFNKDLIKFAIITCRKPQ
Sbjct: 301 RSAFTWKGFTSLLRTGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKPQ 346

[Close]

BLAST of CsaV3_2G036010 vs. ExPASy TrEMBL
Match: A0A6J1FYT6 (tocopherol O-methyltransferase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111449074 PE=3 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 2.1e-162
Identity = 289/349 (82.81%), Postives = 307/349 (87.97%), Query Frame = 0


Query: 1   MNLTAATPIHTSSYPPSSLHSNPFNS--ISPRP---SFSSFTPRRFSPLLASSSAATTPP 60
           M+ T ATPI T SY  SSLHSN FN+  +SP P   SFSSF  RR +  L +++A TT  
Sbjct: 1   MSFTTATPILTPSYSLSSLHSNRFNAAILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60

Query: 61  SLHMDSHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETL 120
            + MD   L KGIAEFYD+SSGLWENVWG+HMHHGFYDP SSVSLSDHRAAQIRMIEETL
Sbjct: 61  EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL 120

Query: 121 RFAGVEAATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVC 180
           RFAGVEAATAVVDVGCGIGGSSRYLA KFGAKCRGITLSPVQAKRAQ I+A  GLSDKVC
Sbjct: 121 RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRV APG TIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240

Query: 241 SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVI 300
           SE SLQPWE KLLQKICDGFYLPAWCSTADYVKLL+SH LQD++ ADWS NVAPFWPAVI
Sbjct: 241 SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI 300

Query: 301 RSAFTWKGFTSLLRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRKPQ 345
           RSAFTW GF+SLLR+GWKTI+GALVMPLMIEGF KDLIKFAIITCRKP+
Sbjct: 301 RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 349

[Close]

BLAST of CsaV3_2G036010 vs. ExPASy TrEMBL
Match: A0A6J1JGD5 (tocopherol O-methyltransferase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111484262 PE=3 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 2.0e-160
Identity = 288/350 (82.29%), Postives = 308/350 (88.00%), Query Frame = 0


Query: 1   MNLTAATPIHTSSYPPSSLHSNPFNS--ISPRP---SF-SSFTPRRFSPLLASSSAATTP 60
           M+ TAATPI T SY  SSLHSN F +  +SP P   SF SSFT  R +  L +++A TT 
Sbjct: 1   MSFTAATPILTPSYSLSSLHSNRFKATILSPIPLRRSFSSSFTRCRRNCSLIATAATTTD 60

Query: 61  PSLHMDSHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEET 120
             + MD  RL +GIAEFYD+SSGLWENVWG+HMHHGFYDP SSVSLSDHRAAQIRMIEET
Sbjct: 61  VEIDMDVDRLKEGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEET 120

Query: 121 LRFAGVEAATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKV 180
           LRFAGVEAATAVVDVGCGIGGSSRYLA KFGAKCRGITLSPVQAKRAQ I+A  GLSDKV
Sbjct: 121 LRFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKV 180

Query: 181 CFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLG 240
           CFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRV APG TIIIVTWCHRDLG
Sbjct: 181 CFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG 240

Query: 241 PSESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAV 300
           PSE SLQPWE KLLQKICDGFYLPAWCSTADYVKL++SH LQD++ ADWS NVAPFWPAV
Sbjct: 241 PSEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLVESHGLQDVRTADWSRNVAPFWPAV 300

Query: 301 IRSAFTWKGFTSLLRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRKPQ 345
           IRSAFTW GF+SLLR+GWKTI+GALVMPLMIEGF KDLIKFAIITCRKP+
Sbjct: 301 IRSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 350

[Close]

BLAST of CsaV3_2G036010 vs. TAIR 10
Match: AT1G64970.1 (gamma-tocopherol methyltransferase )

HSP 1 Score: 448.4 bits (1152), Expect = 5.4e-126
Identity = 228/342 (66.67%), Postives = 263/342 (76.90%), Query Frame = 0


Query: 16  PSSLHSNPFNSISPRPSFSSFTPRRFSPLLASSSAATTP------PSLHMDSHRLHKGIA 75
           PSSL S P+ + S   S SS   R  SP  +SS + TT        +    +  L KGIA
Sbjct: 8   PSSLTSLPYRTNSSFGSKSSLLFR--SPSSSSSVSMTTTRGNVAVAAAATSTEALRKGIA 67

Query: 76  EFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSD--HRAAQIRMIEETLRFAGV------E 135
           EFY+++SGLWE +WG HMHHGFYDP SSV LSD  H+ AQIRMIEE+LRFAGV      +
Sbjct: 68  EFYNETSGLWEEIWGDHMHHGFYDPDSSVQLSDSGHKEAQIRMIEESLRFAGVTDEEEEK 127

Query: 136 AATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVCFEVADA 195
               VVDVGCGIGGSSRYLA+KFGA+C GITLSPVQAKRA +++AA+ L+ K  F+VADA
Sbjct: 128 KIKKVVDVGCGIGGSSRYLASKFGAECIGITLSPVQAKRANDLAAAQSLAHKASFQVADA 187

Query: 196 LNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGPSESSLQ 255
           L+QPF DG+FDLVWSMESGEHMPDK+KFV ELVRV APGG IIIVTWCHR+L   E +LQ
Sbjct: 188 LDQPFEDGKFDLVWSMESGEHMPDKAKFVKELVRVAAPGGRIIIVTWCHRNLSAGEEALQ 247

Query: 256 PWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVIRSAFTW 315
           PWE  +L KIC  FYLPAWCST DYV LLQSH+LQDIK ADWSENVAPFWPAVIR+A TW
Sbjct: 248 PWEQNILDKICKTFYLPAWCSTDDYVNLLQSHSLQDIKCADWSENVAPFWPAVIRTALTW 307

Query: 316 KGFTSLLRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRKP 344
           KG  SLLR+G K+I+GAL MPLMIEG+ K +IKF IITC+KP
Sbjct: 308 KGLVSLLRSGMKSIKGALTMPLMIEGYKKGVIKFGIITCQKP 347

[Close]

BLAST of CsaV3_2G036010 vs. TAIR 10
Match: AT1G20330.1 (sterol methyltransferase 2 )

HSP 1 Score: 84.3 bits (207), Expect = 2.0e-16
Identity = 62/223 (27.80%), Postives = 102/223 (45.74%), Query Frame = 0


Query: 71  FYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETLRFAGVEAATAVVDVG 130
           FY+  + ++E  WGQ  H   + P  S+    H+ A     E  +    V+    ++DVG
Sbjct: 77  FYNLVTDIYEWGWGQSFH---FSP--SIPGKSHKDATRLHEEMAVDLIQVKPGQKILDVG 136

Query: 131 CGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVCFEV-ADALNQPFSDG 190
           CG+GG  R +A+   A   GIT++  Q  RA+  +   GL D +C  V  + L  PF D 
Sbjct: 137 CGVGGPMRAIASHSRANVVGITINEYQVNRARLHNKKAGL-DALCEVVCGNFLQMPFDDN 196

Query: 191 EFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGPSESSLQPWELKLLQ 250
            FD  +S+E+  H P   +  +E+ RV  PG   +   W   +   +E       ++++Q
Sbjct: 197 SFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDD---EHVEVIQ 256

Query: 251 KICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVA-PFW 292
            I  G  LP   +  D  +  +    + +K  D +   A P+W
Sbjct: 257 GIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWW 290

[Close]

BLAST of CsaV3_2G036010 vs. TAIR 10
Match: AT1G76090.1 (sterol methyltransferase 3 )

HSP 1 Score: 82.4 bits (202), Expect = 7.7e-16
Identity = 63/223 (28.25%), Postives = 101/223 (45.29%), Query Frame = 0


Query: 71  FYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETLRFAGVEAATAVVDVG 130
           FY+  + ++E  WGQ  H   + PH     SD  A +I   E  +    V+    ++D G
Sbjct: 77  FYNLVTDIYEWGWGQSFH---FSPHVP-GKSDKDATRIHE-EMAVDLIKVKPGQKILDAG 136

Query: 131 CGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVCFEV-ADALNQPFSDG 190
           CG+GG  R +A    A+  GIT++  Q +RA+  +   GL D +C  V  + L  PF + 
Sbjct: 137 CGVGGPMRAIAAHSKAQVTGITINEYQVQRAKLHNKKAGL-DSLCNVVCGNFLKMPFDEN 196

Query: 191 EFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGPSESSLQPWELKLLQ 250
            FD  +S+E+  H P   +  SE+ RV  PG   +   W   +    +         ++Q
Sbjct: 197 TFDGAYSIEATCHAPKLEEVYSEIFRVMKPGSLFVSYEWVTTEKYRDDDEEHK---DVIQ 256

Query: 251 KICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVA-PFW 292
            I  G  LP   S AD     +    + +K  D ++  + P+W
Sbjct: 257 GIERGDALPGLRSYADIAVTAKKVGFEVVKEKDLAKPPSKPWW 290

[Close]

BLAST of CsaV3_2G036010 vs. TAIR 10
Match: AT5G13710.1 (sterol methyltransferase 1 )

HSP 1 Score: 80.5 bits (197), Expect = 2.9e-15
Identity = 52/167 (31.14%), Postives = 79/167 (47.31%), Query Frame = 0


Query: 68  IAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETLRF-AGVEAATAV 127
           + ++YD ++  +E  WG+  H  F       SL +     I+  E  L    G++    V
Sbjct: 45  VNKYYDLATSFYEYGWGESFH--FAQRWKGESLRE----SIKRHEHFLALQLGIQPGQKV 104

Query: 128 VDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVC-FEVADALNQP 187
           +DVGCGIGG  R +A    +   G+  +  Q  R +E++   G+ DK C F  AD +  P
Sbjct: 105 LDVGCGIGGPLREIARFSNSVVTGLNNNEYQITRGKELNRLAGV-DKTCNFVKADFMKMP 164

Query: 188 FSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRD 233
           F +  FD V+++E+  H PD      E+ RV  PG       WC  D
Sbjct: 165 FPENSFDAVYAIEATCHAPDAYGCYKEIYRVLKPGQCFAAYEWCMTD 204

[Close]

BLAST of CsaV3_2G036010 vs. TAIR 10
Match: AT5G13710.2 (sterol methyltransferase 1 )

HSP 1 Score: 80.5 bits (197), Expect = 2.9e-15
Identity = 52/167 (31.14%), Postives = 79/167 (47.31%), Query Frame = 0


Query: 68  IAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETLRF-AGVEAATAV 127
           + ++YD ++  +E  WG+  H  F       SL +     I+  E  L    G++    V
Sbjct: 45  VNKYYDLATSFYEYGWGESFH--FAQRWKGESLRE----SIKRHEHFLALQLGIQPGQKV 104

Query: 128 VDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVC-FEVADALNQP 187
           +DVGCGIGG  R +A    +   G+  +  Q  R +E++   G+ DK C F  AD +  P
Sbjct: 105 LDVGCGIGGPLREIARFSNSVVTGLNNNEYQITRGKELNRLAGV-DKTCNFVKADFMKMP 164

Query: 188 FSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRD 233
           F +  FD V+++E+  H PD      E+ RV  PG       WC  D
Sbjct: 165 FPENSFDAVYAIEATCHAPDAYGCYKEIYRVLKPGQCFAAYEWCMTD 204

The following BLAST results are available for this feature:

BLAST of CsaV3_2G036010 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004151846.2	1.4e-200	100.00	tocopherol O-methyltransferase, chloroplastic isoform X1 [Cucumis sativus] >KGN6...	[more]
XP_008462787.1	1.4e-176	89.97	PREDICTED: tocopherol O-methyltransferase, chloroplastic [Cucumis melo] >TYK0098...	[more]
XP_038900464.1	2.8e-169	86.53	tocopherol O-methyltransferase, chloroplastic [Benincasa hispida]	[more]
KAG7011011.1	4.4e-162	82.81	Tocopherol O-methyltransferase, chloroplastic [Cucurbita argyrosperma subsp. arg...	[more]
XP_022944690.1	4.4e-162	82.81	tocopherol O-methyltransferase, chloroplastic [Cucurbita moschata]	[more]

BLAST of CsaV3_2G036010 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZSK1	7.5e-125	66.67	Tocopherol O-methyltransferase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q6ZIK0	5.4e-123	67.95	Probable tocopherol O-methyltransferase, chloroplastic OS=Oryza sativa subsp. ja...	[more]
W5U2K2	1.2e-69	45.20	3-hydroxy-16-methoxy-2,3-dihydrotabersonine N-methyltransferase OS=Catharanthus ...	[more]
P74388	2.0e-37	34.49	2-methyl-6-phytyl-1,4-hydroquinone methyltransferase OS=Synechocystis sp. (strai...	[more]
Q8KZ94	5.2e-25	37.11	Demethylrebeccamycin-D-glucose O-methyltransferase OS=Lentzea aerocolonigenes OX...	[more]

BLAST of CsaV3_2G036010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LTM1	6.7e-201	100.00	Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G43...	[more]
A0A5D3BSC2	6.8e-177	89.97	Tocopherol O-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3CHR0	6.8e-177	89.97	tocopherol O-methyltransferase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1035...	[more]
A0A6J1FYT6	2.1e-162	82.81	tocopherol O-methyltransferase, chloroplastic OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1JGD5	2.0e-160	82.29	tocopherol O-methyltransferase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC...	[more]

BLAST of CsaV3_2G036010 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G64970.1	5.4e-126	66.67	gamma-tocopherol methyltransferase	[more]
AT1G20330.1	2.0e-16	27.80	sterol methyltransferase 2	[more]
AT1G76090.1	7.7e-16	28.25	sterol methyltransferase 3	[more]
AT5G13710.1	2.9e-15	31.14	sterol methyltransferase 1	[more]
AT5G13710.2	2.9e-15	31.14	sterol methyltransferase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013216	Methyltransferase type 11	PFAM	PF08241	Methyltransf_11	coord: 127..225 e-value: 2.2E-19 score: 69.9
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 73..314 e-value: 6.4E-57 score: 194.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR43591:SF72	CYCLOPROPANE-FATTY-ACYL-PHOSPHOLIPID SYNTHASE	coord: 60..339
None	No IPR available	PANTHER	PTHR43591	METHYLTRANSFERASE	coord: 60..339
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 126..228 e-value: 1.90334E-14 score: 66.6846
IPR025774	SAM-dependent methyltransferase gTMT-type	PROSITE	PS51581	SAM_GTMT	coord: 54..344 score: 137.029953
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 64..343

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_2G036010.1	CsaV3_2G036010.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0008168	methyltransferase activity