Homology
BLAST of CsaV3_2G035310 vs. NCBI nr
Match:
XP_004151924.2 (cytochrome P450 81Q32 [Cucumis sativus] >KGN63284.1 hypothetical protein Csa_022055 [Cucumis sativus])
HSP 1 Score: 1044.6 bits (2700), Expect = 2.7e-301
Identity = 513/513 (100.00%), Postives = 513/513 (100.00%), Query Frame = 0
Query: 1 MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS
Sbjct: 1 MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
Query: 61 QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120
QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY
Sbjct: 61 QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120
Query: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD 180
GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD
Sbjct: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD 180
Query: 181 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 240
LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW
Sbjct: 181 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 240
Query: 241 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 300
IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG
Sbjct: 241 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 300
Query: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ
Sbjct: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
Query: 361 GIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKP 420
GIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKP
Sbjct: 361 GIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKP 420
Query: 421 ERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMRE 480
ERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMRE
Sbjct: 421 ERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMRE 480
Query: 481 GEGLTMPKVESLVAKCRPRFIMKVVLGEKNGHN 514
GEGLTMPKVESLVAKCRPRFIMKVVLGEKNGHN
Sbjct: 481 GEGLTMPKVESLVAKCRPRFIMKVVLGEKNGHN 513
BLAST of CsaV3_2G035310 vs. NCBI nr
Match:
KAA0032538.1 (isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa])
HSP 1 Score: 951.0 bits (2457), Expect = 4.1e-273
Identity = 467/512 (91.21%), Postives = 484/512 (94.53%), Query Frame = 0
Query: 1 MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
MDFDHLLY LLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKT QTLS
Sbjct: 1 MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
Query: 61 QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120
QKYGHVFSLKFGSRLV+LVSSPSA+QECFTKNDIILANRPSLNSGKYL+YNNTTM VS Y
Sbjct: 61 QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
Query: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD 180
GEHWRNLRRI TLEIFSTTRLNSFSR REEEVKRLL KL GNYK EDEFRVVELE MLLD
Sbjct: 121 GEHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180
Query: 181 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 240
LT NIVMRMVGGKKFC+E KN NVLEDEGYCKR KELVTQIMAH GSTNPGDFIPL NW
Sbjct: 181 LTFNIVMRMVGGKKFCEE--KNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNW 240
Query: 241 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 300
IDPSGY KRIMKIGR+ DEVLQ LVDEIRNEEDEGNTMIQHLLRLQKTDP+YYSD IIKG
Sbjct: 241 IDPSGYTKRIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKG 300
Query: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
L+QDIL+AGIDTSAVTLQWALSHLLNNPIVL+KAKAEIDSYIGQERMVNE DLSSLSYLQ
Sbjct: 301 LVQDILLAGIDTSAVTLQWALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQ 360
Query: 361 GIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKP 420
GIISETLRLSP GPLLVPHC+SEDCKIGGYDVPRNTIVLINAWAIHRDPN+WEDA SFKP
Sbjct: 361 GIISETLRLSPAGPLLVPHCSSEDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKP 420
Query: 421 ERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMRE 480
ERHVNA GFENSYKLLPFG+GRRACPGMAMAQRV+GLTLASL+QCFEWK+MS+LLVDMRE
Sbjct: 421 ERHVNAAGFENSYKLLPFGMGRRACPGMAMAQRVIGLTLASLVQCFEWKRMSDLLVDMRE 480
Query: 481 GEGLTMPKVESLVAKCRPRFIMKVVLGEKNGH 513
GEGLTMPKVE LVAKCRPRFIM+ VL EKNGH
Sbjct: 481 GEGLTMPKVEPLVAKCRPRFIMEAVLSEKNGH 510
BLAST of CsaV3_2G035310 vs. NCBI nr
Match:
XP_038902403.1 (cytochrome P450 81Q32-like [Benincasa hispida])
HSP 1 Score: 856.7 bits (2212), Expect = 1.0e-244
Identity = 418/514 (81.32%), Postives = 454/514 (88.33%), Query Frame = 0
Query: 1 MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
MDFD L LLSL+ LLLGY+ L KP+RLNLPPTPLF LPFIGHLHLLKHPVH+T Q LS
Sbjct: 1 MDFDILFCILLSLVCLLLGYTSLLKPNRLNLPPTPLFCLPFIGHLHLLKHPVHQTFQNLS 60
Query: 61 QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120
QKYGHVFSLKFGSRL++LVSSPS VQECFTKNDIILANRPSLN+GKYL+YNN TMAVS Y
Sbjct: 61 QKYGHVFSLKFGSRLIVLVSSPSVVQECFTKNDIILANRPSLNTGKYLAYNNITMAVSSY 120
Query: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGN-YKFEDEFRVVELESMLL 180
GEHWRNLRRI TLEIFS TR+NSF R RE+EVKRLLCKLCGN Y ED+FR V+L+ MLL
Sbjct: 121 GEHWRNLRRISTLEIFSMTRVNSFLRTREDEVKRLLCKLCGNCYTLEDKFRAVDLKPMLL 180
Query: 181 DLTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWN 240
DLT NIVMRMVGGKKFC+ENN N NVLEDEGY KR +E+VTQIMAH+GSTNP DFIPLWN
Sbjct: 181 DLTFNIVMRMVGGKKFCEENNNN-NVLEDEGYSKRFREVVTQIMAHSGSTNPEDFIPLWN 240
Query: 241 WIDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIK 300
WIDP+G+NKRIMK+GRR DE+LQ LVDEIRN+EDEGN MI HLLRLQ TD +YYSD +IK
Sbjct: 241 WIDPTGFNKRIMKLGRRADEILQGLVDEIRNQEDEGNNMIHHLLRLQNTDLEYYSDQVIK 300
Query: 301 GLIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYL 360
GLI DILIAGIDT+AVTL+WALSHLLNNPIVL++AKAEID IGQERMVNE DLSSLSYL
Sbjct: 301 GLIHDILIAGIDTTAVTLEWALSHLLNNPIVLERAKAEIDCSIGQERMVNEADLSSLSYL 360
Query: 361 QGIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFK 420
QGIISETLRLSPPGPLLVPHCASEDCKIGGYD+PR TIVLIN WAIHRDPN+WEDA SFK
Sbjct: 361 QGIISETLRLSPPGPLLVPHCASEDCKIGGYDIPRKTIVLINVWAIHRDPNLWEDATSFK 420
Query: 421 PERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMR 480
PERH N E SYKLLPFGLGRRACPGMAMAQR++GLTLASLIQCFEWK+ S LVDM
Sbjct: 421 PERHANVAEVEYSYKLLPFGLGRRACPGMAMAQRIIGLTLASLIQCFEWKRTSKSLVDMS 480
Query: 481 EGEGLTMPKVESLVAKCRPRFIMKVVLGEKNGHN 514
EG+GLTMPKVE ++AKCRPR IMK VL E N
Sbjct: 481 EGKGLTMPKVEPIMAKCRPRLIMKAVLSEMTSQN 513
BLAST of CsaV3_2G035310 vs. NCBI nr
Match:
XP_038902241.1 (cytochrome P450 81Q32-like [Benincasa hispida])
HSP 1 Score: 839.3 bits (2167), Expect = 1.7e-239
Identity = 412/508 (81.10%), Postives = 446/508 (87.80%), Query Frame = 0
Query: 1 MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
MDFD LL LLSL+ LLLGYSFL KPH LNLPPTPLF LPFIGHLHLLKHPVHK Q LS
Sbjct: 1 MDFDLLLCILLSLVYLLLGYSFLLKPHGLNLPPTPLFCLPFIGHLHLLKHPVHKIFQNLS 60
Query: 61 QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120
QKYGHVFSLKFGS LV+LVSSPSAVQECFTKNDIILANRPSLN GKYL+YNNT+MA+SPY
Sbjct: 61 QKYGHVFSLKFGSHLVVLVSSPSAVQECFTKNDIILANRPSLNPGKYLAYNNTSMALSPY 120
Query: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD 180
GEHWRNLRRI TLEIFS TR N F + RE+EVKRLL KLCGN EDEFRVVELE MLLD
Sbjct: 121 GEHWRNLRRISTLEIFSATRFNLFLKSREDEVKRLLYKLCGNDTLEDEFRVVELEPMLLD 180
Query: 181 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 240
LT NIVMRMVGGKKFC+ENNKN++ EDEGY KR +ELVTQIMAH GSTNPGDFIPLWNW
Sbjct: 181 LTFNIVMRMVGGKKFCEENNKNVS--EDEGYSKRFRELVTQIMAHGGSTNPGDFIPLWNW 240
Query: 241 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 300
IDP+GYNKR+MK+GRR DEVLQ L+DEIRN+EDEG MIQHLLRLQ TDP+YYSD +IKG
Sbjct: 241 IDPTGYNKRLMKLGRRSDEVLQGLIDEIRNQEDEGINMIQHLLRLQNTDPEYYSDQVIKG 300
Query: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
L+QDIL+ GIDTSAVTL+WALSHLLNNPIVL++AKAEID +IGQERMVNE DLSSLSYLQ
Sbjct: 301 LVQDILLGGIDTSAVTLEWALSHLLNNPIVLERAKAEIDCFIGQERMVNEADLSSLSYLQ 360
Query: 361 GIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKP 420
GIISETLRLSPPGPLL PH AS+DC IGGYDVPRNT+VL N WAIHRDPN+WEDA SFKP
Sbjct: 361 GIISETLRLSPPGPLL-PHYASKDCTIGGYDVPRNTMVLFNIWAIHRDPNLWEDATSFKP 420
Query: 421 ERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMRE 480
ERH N E SYKLLPFGLGRRACPGMA+AQRV+GLTLASLIQCFEWK+ S LVDM E
Sbjct: 421 ERHANVAEVEYSYKLLPFGLGRRACPGMAIAQRVIGLTLASLIQCFEWKRTSKSLVDMSE 480
Query: 481 GEGLTMPKVESLVAKCRPRFIMKVVLGE 509
G+GLTMPK + L+ K RP IMK +L E
Sbjct: 481 GKGLTMPKAKPLIVKYRPCLIMKAILSE 505
BLAST of CsaV3_2G035310 vs. NCBI nr
Match:
XP_008455822.2 (PREDICTED: LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like [Cucumis melo])
HSP 1 Score: 815.8 bits (2106), Expect = 2.0e-232
Identity = 407/496 (82.06%), Postives = 439/496 (88.51%), Query Frame = 0
Query: 1 MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
MDFDHLLY LLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKT QTLS
Sbjct: 1 MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
Query: 61 QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120
QKYGHVFSLKFGSRLV+LVSSPSA+QECFTKNDIILANRPSLNSGKYL+YNNTTM VS Y
Sbjct: 61 QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
Query: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD 180
G+HWRNLRRI TLEIFSTTRLNSFSR REEEVKRLL KL GNYK EDEFRVVELE MLLD
Sbjct: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180
Query: 181 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 240
LT NIVMRMVGGKKFC+E KN NVLEDEGYCKR KELVTQIMAH GSTNPGDFIPL NW
Sbjct: 181 LTFNIVMRMVGGKKFCEE--KNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNW 240
Query: 241 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 300
IDPSGY K IMKIGR+ DEVLQ LVDEIRNEEDEGNTMIQHLLRLQKTDP+YYSD IIKG
Sbjct: 241 IDPSGYTKNIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKG 300
Query: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
L+QDI++AGIDTS ++L+WALS LLNNP VL KAK EID IG +R+V+EVDL SLSYLQ
Sbjct: 301 LVQDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQ 360
Query: 361 GIISETLRLSPPGPLLVPHCA-SEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFK 420
GIISETLRL PP PLLVPHCA E+CKI Y++PR+TIVLINAWAIHRD +WEDA FK
Sbjct: 361 GIISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFK 420
Query: 421 PERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMR 480
PERH +V YKL+PFG+GRRACPG MAQRVVGL LASLIQ FEW+++ + L+DM
Sbjct: 421 PERH--SVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMS 480
Query: 481 EGEGLTMPKVESLVAK 496
EG+GLTMPK + L+AK
Sbjct: 481 EGKGLTMPKAQPLIAK 492
BLAST of CsaV3_2G035310 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 528.1 bits (1359), Expect = 1.1e-148
Identity = 266/509 (52.26%), Postives = 351/509 (68.96%), Query Frame = 0
Query: 1 MDFDHLLYALLSLISLLLGYS-FLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTL 60
M+ LLY L+++ L + F R NLPP+P LP IGHLHL+ +H++L L
Sbjct: 1 MESSTLLYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDL 60
Query: 61 SQKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSP 120
S+KYG VFSL+ G+RLVL+VSSP+A +ECFTKNDI+ ANRP GKY+ YN TTM SP
Sbjct: 61 SKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSP 120
Query: 121 YGEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLL 180
YGEHWRNLRR+ +EIFS LN F IRE+EVK+LL L +F VE++S L
Sbjct: 121 YGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSL--YQSSGQDFGKVEMKSKLS 180
Query: 181 DLTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWN 240
+L+ N+ MRMV GK++ ++ D K + L+ ++ HAG++NPGDF+P
Sbjct: 181 ELSFNVTMRMVAGKRYFGQD-------VDSDEAKLFRALIGEVFEHAGASNPGDFVPFLR 240
Query: 241 WIDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIK 300
WID Y K++ KI + MD LQRL+ E R ++ TMI HLL LQ++ P+YY+D IIK
Sbjct: 241 WIDFKNYEKKVSKISQEMDAFLQRLIHESRINKN-NVTMIDHLLSLQESQPEYYTDQIIK 300
Query: 301 GLIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYL 360
G+I +L+AG DTSAVT++WA+S LLN+P L+KA+ EI++ +G R++ E DL L+YL
Sbjct: 301 GIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYL 360
Query: 361 QGIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFK 420
IISET RL P P+LVPH +S+DCK+ GYDVP+ TI+L+NAWAIHRDP W++ FK
Sbjct: 361 HNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFK 420
Query: 421 PERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMR 480
PERH V E S KL+PFG+GRR+CPG +AQRVVGLTL +LIQCFEWK++ +DM
Sbjct: 421 PERH-GGVELEPS-KLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMA 480
Query: 481 EGEGLTMPKVESLVAKCRPRFIMKVVLGE 509
EG GLTMPK + L A C+PR I+ V+ E
Sbjct: 481 EGSGLTMPKAQPLEALCKPRNILHKVVSE 497
BLAST of CsaV3_2G035310 vs. ExPASy Swiss-Prot
Match:
Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)
HSP 1 Score: 516.5 bits (1329), Expect = 3.4e-145
Identity = 269/510 (52.75%), Postives = 356/510 (69.80%), Query Frame = 0
Query: 6 LLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQKYGH 65
+LY++ SLI L++ + FL KP + NLPP+P LP IGHL LLK P+H+TL++ S+ H
Sbjct: 9 VLYSIFSLIFLIISFKFL-KPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 68
Query: 66 -----VFSLKFGSRLVLLVSSPS-AVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSP 125
V SL+ GSRLV +VSS A +ECF KND++LANRP + GK++ YNNT M +P
Sbjct: 69 NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 128
Query: 126 YGEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRL---LCKLCGNYKFEDEFRVVELES 185
YG+HWRNLRR+CT+EIFST RLN F +R +EV+RL L +L G K VVEL+
Sbjct: 129 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKK-----TVVELKP 188
Query: 186 MLLDLTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIP 245
ML+DLT N +MRM+ GK++ E DE KR+++LV + A+ S N D++P
Sbjct: 189 MLMDLTFNNIMRMMTGKRYYGEET------TDEEEAKRVRKLVADVGANTSSGNAVDYVP 248
Query: 246 LWNWIDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDL 305
+ S Y R+ K+G D+ LQ L+D+ R +++ G TMI HLL LQK+D +YY+D
Sbjct: 249 ILRLF--SSYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQ 308
Query: 306 IIKGLIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSL 365
IIKG+I ++IAG +TSAVTL+WALS+LLN+P V+ KA+ EID+ +G +R++ E DLS L
Sbjct: 309 IIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSEL 368
Query: 366 SYLQGIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAG 425
YL+ I+ ETLRL P PLLVPH ASEDCKIG YD+PR T +L+NAWAIHRDPN W+D
Sbjct: 369 PYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPD 428
Query: 426 SFKPERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLV 485
SFKPER E + KLL FGLGRRACPG +AQR+VGL L SLIQCFEW+++ N+ V
Sbjct: 429 SFKPERFEKE---EEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEV 488
Query: 486 DMREGEGLTMPKVESLVAKCRPR-FIMKVV 506
DM+EG G T+PK L A C+ R F+ K++
Sbjct: 489 DMKEGVGNTVPKAIPLKAICKARPFLHKII 501
BLAST of CsaV3_2G035310 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 489.2 bits (1258), Expect = 5.7e-137
Identity = 256/496 (51.61%), Postives = 341/496 (68.75%), Query Frame = 0
Query: 8 YALLSLISLLLGYSFLFK--PHRL-NLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQKYG 67
Y+LLSL S ++ L K RL NLPP P +P IG+LH LKHP+H+T TLSQ YG
Sbjct: 5 YSLLSL-SFIITIKILLKITSRRLKNLPPGPP-TIPIIGNLHHLKHPLHRTFTTLSQTYG 64
Query: 68 HVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPYGEHW 127
+FSL FGSRLV++VSSPS ECFTKNDIILANRP +GKY+ YN TT+ + YG+HW
Sbjct: 65 DIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHW 124
Query: 128 RNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFED-EFRVVELESMLLDLTS 187
RNLRRI T+++ S RLNSF +R +E RL+ KL + E F VEL L ++T
Sbjct: 125 RNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTF 184
Query: 188 NIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNWIDP 247
N +MRM+ GK++ ++ +V E K+ +E+++++M+ G+ N GDF+PL +D
Sbjct: 185 NAMMRMISGKRYYGDDGDVSDVEE----AKQFREIISEMMSLLGANNKGDFLPLLRVVDL 244
Query: 248 SGYNKRIMKIGRRMDEVLQRLVDEIR--NEEDEGNTMIQHLLRLQKTDPKYYSDLIIKGL 307
KR +I +R + L+ L++E R N +G TMI HLL+L ++ P+YYSD +IKGL
Sbjct: 245 DNLEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGL 304
Query: 308 IQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQG 367
IQ +L+AG DTSAVT++W +S LLN+P VL KAK E+D+ IG+ ++V+E DLS L YLQ
Sbjct: 305 IQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQN 364
Query: 368 IISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKPE 427
IISETLRL PP PLL+PH +SEDC IG ++VP++TI+L N W IHRDP W DA SFKPE
Sbjct: 365 IISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPE 424
Query: 428 RHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMREG 487
R E K++ FGLGRRACPG+++AQR VG T+ LIQCFEW++ S +DM EG
Sbjct: 425 RFEKE---EEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEG 484
Query: 488 EGLTMPKVESLVAKCR 498
+G+TMP L A C+
Sbjct: 485 KGITMPMKIPLRAMCK 491
BLAST of CsaV3_2G035310 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 488.0 bits (1255), Expect = 1.3e-136
Identity = 254/503 (50.50%), Postives = 342/503 (67.99%), Query Frame = 0
Query: 7 LYALLSLISLLLGYSFLFKPHR--LNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQKYG 66
L ++SL L++ F R NLPP P LP IG+LH LK P+H T TLSQKYG
Sbjct: 6 LSLIISLFFLIITLKVFFNTSRKFKNLPPGPQC-LPIIGNLHQLKQPLHHTFHTLSQKYG 65
Query: 67 HVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPYGEHW 126
+FSL FGSRLV++VSS + QECFTKNDI+LANRP +GKY+ YNNTT+A SPYG+HW
Sbjct: 66 QIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHW 125
Query: 127 RNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLDLTSN 186
RNLRRI ++EI S+ RLNSF IR +E+ RL+ KL K + F VEL M ++T N
Sbjct: 126 RNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLA--QKSYNGFTEVELRPMFSEMTFN 185
Query: 187 IVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNWIDPS 246
+MRMV GK++ N+ +++ +E+ + + ++ ++++ G+ N GDF+ W D
Sbjct: 186 TIMRMVSGKRY-YGNDCDVSDVEE---ARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFD 245
Query: 247 GYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKGLIQD 306
G KR+ KI +R D LQ L+DE R + NTMI HLL Q++ P+YY+D IIKGL+
Sbjct: 246 GLEKRLKKISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVV 305
Query: 307 ILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQGIIS 366
+L+AG DTS+VT++WA+S+LLN+P ++ KAK E+D++IG +R V+E D+S L YLQ I+
Sbjct: 306 MLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVY 365
Query: 367 ETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKPERHV 426
ETLRL PLLVPH +SED +GGY++P+NTI+++NAW IHRDPN+W D FKPER
Sbjct: 366 ETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPER-- 425
Query: 427 NAVGFENS---YKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMREG 486
FE KLL FGLGRRACPG ++QR GLTL LIQCFEWK++ +DM E
Sbjct: 426 ----FEKEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEA 485
Query: 487 EGLTMPKVESLVAKCRPRFIMKV 505
+G+T K SL A C+ R +K+
Sbjct: 486 KGITAGKKTSLNAMCKVRHPLKI 495
BLAST of CsaV3_2G035310 vs. ExPASy Swiss-Prot
Match:
O65790 (Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2)
HSP 1 Score: 486.5 bits (1251), Expect = 3.7e-136
Identity = 243/507 (47.93%), Postives = 354/507 (69.82%), Query Frame = 0
Query: 6 LLYALLSLISLLLGYSFLF-KPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQKYG 65
L + LL L+ L++ Y FL+ K R NLPP P PF+GHLHL+K P+H+ LQ S +YG
Sbjct: 2 LYFILLPLLFLVISYKFLYSKTQRFNLPPGPP-SRPFVGHLHLMKPPIHRLLQRYSNQYG 61
Query: 66 HVFSLKFGSRLVLLVSSPSAVQECFT-KNDIILANRPSLNSGKYLSYNNTTMAVSPYGEH 125
+FSL+FGSR V++++SPS QE FT +NDI+L++RP + KY++YN+TT+ +PYG+H
Sbjct: 62 PIFSLRFGSRRVVVITSPSLAQESFTGQNDIVLSSRPLQLTAKYVAYNHTTVGTAPYGDH 121
Query: 126 WRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDE---FRVVELESMLLD 185
WRNLRRIC+ EI S+ RL +F IR++E+ R+L +L + +E F +ELE +L D
Sbjct: 122 WRNLRRICSQEILSSHRLINFQHIRKDEILRMLTRLSRYTQTSNESNDFTHIELEPLLSD 181
Query: 186 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 245
LT N ++RMV GK++ ++ N E K+LV I ++G+ + D++P+
Sbjct: 182 LTFNNIVRMVTGKRYYGDDVNNKEEAE------LFKKLVYDIAMYSGANHSADYLPILK- 241
Query: 246 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 305
+ + + K + IG+ MD++LQRL+DE R ++ EGNTM+ HL+ LQ+ P+YY+D+IIKG
Sbjct: 242 LFGNKFEKEVKAIGKSMDDILQRLLDECRRDK-EGNTMVNHLISLQQQQPEYYTDVIIKG 301
Query: 306 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 365
L+ +++AG +TSAVTL+WA+++LL NP VL+KA++EID IG++R+++E D++ L YLQ
Sbjct: 302 LMMSMMLAGTETSAVTLEWAMANLLRNPEVLEKARSEIDEKIGKDRLIDESDIAVLPYLQ 361
Query: 366 GIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKP 425
++SET RL P P L+P ++D KIGGYDVPR+TIV++NAWAIHRDP +WE+ F P
Sbjct: 362 NVVSETFRLFPVAPFLIPRSPTDDMKIGGYDVPRDTIVMVNAWAIHRDPEIWEEPEKFNP 421
Query: 426 ERHVNAVGFE-NSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMR 485
+R+ + G + YKL+PFG GRR CPG + QR+V L L SLIQCFEW+ + +DM
Sbjct: 422 DRYNDGCGSDYYVYKLMPFGNGRRTCPGAGLGQRIVTLALGSLIQCFEWENVKGEEMDMS 481
Query: 486 EGEGLTMPKVESLVAKCRPRFIMKVVL 507
E GL M K++ L A CRPR IM +L
Sbjct: 482 ESTGLGMRKMDPLRAMCRPRPIMSKLL 499
BLAST of CsaV3_2G035310 vs. ExPASy TrEMBL
Match:
A0A0A0LQI5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1)
HSP 1 Score: 1044.6 bits (2700), Expect = 1.3e-301
Identity = 513/513 (100.00%), Postives = 513/513 (100.00%), Query Frame = 0
Query: 1 MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS
Sbjct: 1 MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
Query: 61 QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120
QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY
Sbjct: 61 QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120
Query: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD 180
GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD
Sbjct: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD 180
Query: 181 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 240
LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW
Sbjct: 181 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 240
Query: 241 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 300
IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG
Sbjct: 241 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 300
Query: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ
Sbjct: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
Query: 361 GIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKP 420
GIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKP
Sbjct: 361 GIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKP 420
Query: 421 ERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMRE 480
ERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMRE
Sbjct: 421 ERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMRE 480
Query: 481 GEGLTMPKVESLVAKCRPRFIMKVVLGEKNGHN 514
GEGLTMPKVESLVAKCRPRFIMKVVLGEKNGHN
Sbjct: 481 GEGLTMPKVESLVAKCRPRFIMKVVLGEKNGHN 513
BLAST of CsaV3_2G035310 vs. ExPASy TrEMBL
Match:
A0A5A7SPU0 (Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold465G00500 PE=3 SV=1)
HSP 1 Score: 951.0 bits (2457), Expect = 2.0e-273
Identity = 467/512 (91.21%), Postives = 484/512 (94.53%), Query Frame = 0
Query: 1 MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
MDFDHLLY LLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKT QTLS
Sbjct: 1 MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
Query: 61 QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120
QKYGHVFSLKFGSRLV+LVSSPSA+QECFTKNDIILANRPSLNSGKYL+YNNTTM VS Y
Sbjct: 61 QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
Query: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD 180
GEHWRNLRRI TLEIFSTTRLNSFSR REEEVKRLL KL GNYK EDEFRVVELE MLLD
Sbjct: 121 GEHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180
Query: 181 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 240
LT NIVMRMVGGKKFC+E KN NVLEDEGYCKR KELVTQIMAH GSTNPGDFIPL NW
Sbjct: 181 LTFNIVMRMVGGKKFCEE--KNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNW 240
Query: 241 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 300
IDPSGY KRIMKIGR+ DEVLQ LVDEIRNEEDEGNTMIQHLLRLQKTDP+YYSD IIKG
Sbjct: 241 IDPSGYTKRIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKG 300
Query: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
L+QDIL+AGIDTSAVTLQWALSHLLNNPIVL+KAKAEIDSYIGQERMVNE DLSSLSYLQ
Sbjct: 301 LVQDILLAGIDTSAVTLQWALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQ 360
Query: 361 GIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKP 420
GIISETLRLSP GPLLVPHC+SEDCKIGGYDVPRNTIVLINAWAIHRDPN+WEDA SFKP
Sbjct: 361 GIISETLRLSPAGPLLVPHCSSEDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKP 420
Query: 421 ERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMRE 480
ERHVNA GFENSYKLLPFG+GRRACPGMAMAQRV+GLTLASL+QCFEWK+MS+LLVDMRE
Sbjct: 421 ERHVNAAGFENSYKLLPFGMGRRACPGMAMAQRVIGLTLASLVQCFEWKRMSDLLVDMRE 480
Query: 481 GEGLTMPKVESLVAKCRPRFIMKVVLGEKNGH 513
GEGLTMPKVE LVAKCRPRFIM+ VL EKNGH
Sbjct: 481 GEGLTMPKVEPLVAKCRPRFIMEAVLSEKNGH 510
BLAST of CsaV3_2G035310 vs. ExPASy TrEMBL
Match:
A0A1S3C1S2 (LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103495921 PE=3 SV=1)
HSP 1 Score: 815.8 bits (2106), Expect = 9.9e-233
Identity = 407/496 (82.06%), Postives = 439/496 (88.51%), Query Frame = 0
Query: 1 MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
MDFDHLLY LLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKT QTLS
Sbjct: 1 MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
Query: 61 QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120
QKYGHVFSLKFGSRLV+LVSSPSA+QECFTKNDIILANRPSLNSGKYL+YNNTTM VS Y
Sbjct: 61 QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
Query: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD 180
G+HWRNLRRI TLEIFSTTRLNSFSR REEEVKRLL KL GNYK EDEFRVVELE MLLD
Sbjct: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180
Query: 181 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 240
LT NIVMRMVGGKKFC+E KN NVLEDEGYCKR KELVTQIMAH GSTNPGDFIPL NW
Sbjct: 181 LTFNIVMRMVGGKKFCEE--KNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNW 240
Query: 241 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 300
IDPSGY K IMKIGR+ DEVLQ LVDEIRNEEDEGNTMIQHLLRLQKTDP+YYSD IIKG
Sbjct: 241 IDPSGYTKNIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKG 300
Query: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
L+QDI++AGIDTS ++L+WALS LLNNP VL KAK EID IG +R+V+EVDL SLSYLQ
Sbjct: 301 LVQDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQ 360
Query: 361 GIISETLRLSPPGPLLVPHCA-SEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFK 420
GIISETLRL PP PLLVPHCA E+CKI Y++PR+TIVLINAWAIHRD +WEDA FK
Sbjct: 361 GIISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFK 420
Query: 421 PERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMR 480
PERH +V YKL+PFG+GRRACPG MAQRVVGL LASLIQ FEW+++ + L+DM
Sbjct: 421 PERH--SVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMS 480
Query: 481 EGEGLTMPKVESLVAK 496
EG+GLTMPK + L+AK
Sbjct: 481 EGKGLTMPKAQPLIAK 492
BLAST of CsaV3_2G035310 vs. ExPASy TrEMBL
Match:
A0A1S3C323 (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495924 PE=3 SV=1)
HSP 1 Score: 781.9 bits (2018), Expect = 1.6e-222
Identity = 383/509 (75.25%), Postives = 435/509 (85.46%), Query Frame = 0
Query: 6 LLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQKYGH 65
LL L+S++ + FL +P LNLPPTPLF LP IGHLHLLKHPVH+TLQ LSQKYGH
Sbjct: 5 LLSISFFLLSVVFAFKFLLRPRWLNLPPTPLFWLPLIGHLHLLKHPVHQTLQNLSQKYGH 64
Query: 66 VFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPYGEHWR 125
VFSL+FGSRLV++VSSPSAVQECFTKNDIILANRP L SGKYL YN++++ +SPYGE WR
Sbjct: 65 VFSLRFGSRLVVVVSSPSAVQECFTKNDIILANRPLLESGKYLGYNHSSIGLSPYGEQWR 124
Query: 126 NLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNY-KFE-DEFRVVELESMLLDLTS 185
NLRRI +LE+FST RLN F IRE+EVKRLL KLCGN+ K E DEFRVVE+ +L DLT
Sbjct: 125 NLRRIGSLEVFSTKRLNMFLGIREDEVKRLLRKLCGNHCKLEDDEFRVVEIGPLLKDLTL 184
Query: 186 NIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNWIDP 245
NIVMRMV GK F +E++ + +E+VTQIM HAG++NPGDFIPLWNWIDP
Sbjct: 185 NIVMRMVSGKMFYEEDS------------NKFREIVTQIMEHAGASNPGDFIPLWNWIDP 244
Query: 246 SGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKGLIQ 305
+G+ K++ K+G+ DE LQ+L+D IRN+ D GNTMI HLL LQ +P++Y+D K +IQ
Sbjct: 245 TGFMKKVKKLGKTSDEFLQQLIDGIRNQNDGGNTMIHHLLTLQNVEPEFYNDHAFKAIIQ 304
Query: 306 DILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQGII 365
DIL+AGIDTSAVTLQWALSHLLNNPIVL+KAKAEIDSYIGQERMVNE DLSSLSYLQGII
Sbjct: 305 DILLAGIDTSAVTLQWALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQGII 364
Query: 366 SETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKPERH 425
SETLRLSP GPLLVPHC+SEDCKIGGYDVPRNTIVLINAWAIHRDPN+WEDA SFKPERH
Sbjct: 365 SETLRLSPAGPLLVPHCSSEDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKPERH 424
Query: 426 VNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMREGEG 485
VNA GFENSYKLLPFG+GRRACPGMAMAQRV+GLTLASL+QCFEWK+MS+LLVDMREGEG
Sbjct: 425 VNAAGFENSYKLLPFGMGRRACPGMAMAQRVIGLTLASLVQCFEWKRMSDLLVDMREGEG 484
Query: 486 LTMPKVESLVAKCRPRFIMKVVLGEKNGH 513
LTMPKVE LVAKCRP FIM+ VL EKNGH
Sbjct: 485 LTMPKVEPLVAKCRPCFIMEAVLSEKNGH 501
BLAST of CsaV3_2G035310 vs. ExPASy TrEMBL
Match:
A0A5D3BGH3 (Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold232G00710 PE=3 SV=1)
HSP 1 Score: 739.2 bits (1907), Expect = 1.2e-209
Identity = 380/496 (76.61%), Postives = 412/496 (83.06%), Query Frame = 0
Query: 1 MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
MDFDHLLY LLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKT QTLS
Sbjct: 1 MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
Query: 61 QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120
QKYGHVFSLKFGSRLV+LVSSPSA+QECFTKNDIILANRPSLNSGKYL+YNNTTM VS Y
Sbjct: 61 QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
Query: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD 180
G+HWRNLRRI TLEIFSTTRLNSFSR REEEVKRLL KL GNYK EDEFRVVELE MLLD
Sbjct: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180
Query: 181 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 240
LT NIVMRMVGGKKFC+E KN NVLEDEGYCKR KEL
Sbjct: 181 LTFNIVMRMVGGKKFCEE--KNNNVLEDEGYCKRFKEL---------------------- 240
Query: 241 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 300
IMKIGR+ DEVLQ LVDEIRNEEDEGNTMIQHLLRLQKTDP+YYSD IIKG
Sbjct: 241 --------DIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKG 300
Query: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
L+QDI++AGIDTS ++L+WALS LLNNP VL KAK EID IG +R+V+EVDL SLSYLQ
Sbjct: 301 LVQDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQ 360
Query: 361 GIISETLRLSPPGPLLVPHCA-SEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFK 420
GIISETLRL PP PLLVPHCA E+CKI Y++PR+TIVLINAWAIHRD +WEDA FK
Sbjct: 361 GIISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFK 420
Query: 421 PERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMR 480
PERH +V YKL+PFG+GRRACPG MAQRVVGL LASLIQ FEW+++ + L+DM
Sbjct: 421 PERH--SVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMS 462
Query: 481 EGEGLTMPKVESLVAK 496
EG+GLTMPK + L+AK
Sbjct: 481 EGKGLTMPKAQPLIAK 462
BLAST of CsaV3_2G035310 vs. TAIR 10
Match:
AT5G36220.1 (cytochrome p450 81d1 )
HSP 1 Score: 516.5 bits (1329), Expect = 2.4e-146
Identity = 269/510 (52.75%), Postives = 356/510 (69.80%), Query Frame = 0
Query: 6 LLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQKYGH 65
+LY++ SLI L++ + FL KP + NLPP+P LP IGHL LLK P+H+TL++ S+ H
Sbjct: 9 VLYSIFSLIFLIISFKFL-KPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 68
Query: 66 -----VFSLKFGSRLVLLVSSPS-AVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSP 125
V SL+ GSRLV +VSS A +ECF KND++LANRP + GK++ YNNT M +P
Sbjct: 69 NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 128
Query: 126 YGEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRL---LCKLCGNYKFEDEFRVVELES 185
YG+HWRNLRR+CT+EIFST RLN F +R +EV+RL L +L G K VVEL+
Sbjct: 129 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKK-----TVVELKP 188
Query: 186 MLLDLTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIP 245
ML+DLT N +MRM+ GK++ E DE KR+++LV + A+ S N D++P
Sbjct: 189 MLMDLTFNNIMRMMTGKRYYGEET------TDEEEAKRVRKLVADVGANTSSGNAVDYVP 248
Query: 246 LWNWIDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDL 305
+ S Y R+ K+G D+ LQ L+D+ R +++ G TMI HLL LQK+D +YY+D
Sbjct: 249 ILRLF--SSYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQ 308
Query: 306 IIKGLIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSL 365
IIKG+I ++IAG +TSAVTL+WALS+LLN+P V+ KA+ EID+ +G +R++ E DLS L
Sbjct: 309 IIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSEL 368
Query: 366 SYLQGIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAG 425
YL+ I+ ETLRL P PLLVPH ASEDCKIG YD+PR T +L+NAWAIHRDPN W+D
Sbjct: 369 PYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPD 428
Query: 426 SFKPERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLV 485
SFKPER E + KLL FGLGRRACPG +AQR+VGL L SLIQCFEW+++ N+ V
Sbjct: 429 SFKPERFEKE---EEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEV 488
Query: 486 DMREGEGLTMPKVESLVAKCRPR-FIMKVV 506
DM+EG G T+PK L A C+ R F+ K++
Sbjct: 489 DMKEGVGNTVPKAIPLKAICKARPFLHKII 501
BLAST of CsaV3_2G035310 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 495.4 bits (1274), Expect = 5.7e-140
Identity = 255/496 (51.41%), Postives = 352/496 (70.97%), Query Frame = 0
Query: 6 LLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQKYGH 65
+L+ +LSLI L+ KP NLPP+P + LP IGHL LLK P+H+T +LSQ +
Sbjct: 10 ILFVVLSLIYLI--GKLKRKP---NLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNN 69
Query: 66 --VFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPYGEH 125
+FSL+ G+RLV + SS S +ECFTKND++LANRP+ K+++Y+ TTM + YG+H
Sbjct: 70 APIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDH 129
Query: 126 WRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLDLTS 185
WRNLRRI ++EIFS RLNSF IR++E++RL+ +L N F EF V+++SML DLT
Sbjct: 130 WRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRN--FSQEFVKVDMKSMLSDLTF 189
Query: 186 NIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNWIDP 245
N ++RMV GK++ + +ED+ KR+++L+ ++A AG+ N D++P+ +
Sbjct: 190 NNILRMVAGKRYYGDG------VEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV-- 249
Query: 246 SGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKGLIQ 305
S Y R+ K+ R+DE LQ LVDE R +++GNTMI HLL LQ++ P Y++D IIKG +
Sbjct: 250 SDYETRVKKLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNML 309
Query: 306 DILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQGII 365
+++AG DTSAVTL+WALS++LN+P VL+KA+ EID IG +R+++E D+S+L YLQ I+
Sbjct: 310 ALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIV 369
Query: 366 SETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKPERH 425
SETLRL P P+L+PH ASEDCK+ GYD+PR TI+L N WAIHRDP +W+D SFKPER
Sbjct: 370 SETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERF 429
Query: 426 VNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMREGEG 485
+ KL+PFGLGRRACPG +A R++ LTL SLIQC EW+K+ VDM EG+G
Sbjct: 430 EKE---GEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGE-EVDMSEGKG 486
Query: 486 LTMPKVESLVAKCRPR 500
+TMPK + L A CR R
Sbjct: 490 VTMPKAKPLEAMCRAR 486
BLAST of CsaV3_2G035310 vs. TAIR 10
Match:
AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )
HSP 1 Score: 488.4 bits (1256), Expect = 7.0e-138
Identity = 252/482 (52.28%), Postives = 334/482 (69.29%), Query Frame = 0
Query: 28 RLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQKYGH--VFSLKFGSRLVLLVSSPSAV 87
+LNLPP+P + LP IGHL LLK P+H+ ++SQ G + SL+ G+RL+ +VSS S
Sbjct: 27 KLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAPIISLRLGNRLLFVVSSHSIA 86
Query: 88 QECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPYGEHWRNLRRICTLEIFSTTRLNSFS 147
+ECFTKND+ILANR + S K++SY N+T+ + Y EHWRNLRRI LEIFS RLNSFS
Sbjct: 87 EECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWRNLRRIGALEIFSAHRLNSFS 146
Query: 148 RIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLDLTSNIVMRMVGGKKFCKENNKNINV 207
IR +E++RL+ +L N + F VE++SM DLT N ++RM+ GK + +
Sbjct: 147 SIRRDEIRRLIGRLLRNSSY--GFTKVEMKSMFSDLTFNNIIRMLAGKCYYGDGK----- 206
Query: 208 LEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNWIDPSGYNKRIMKIGRRMDEVLQRLV 267
ED+ KR++ L+ + M+ +G N D+IP+ WI S RI K+ R+DE LQ LV
Sbjct: 207 -EDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS--ETRIKKLAGRLDEFLQGLV 266
Query: 268 DEIR-NEEDEGNTMIQHLLRLQKTDPKYYSDLIIKGLIQDILIAGIDTSAVTLQWALSHL 327
DE R +E + NTM+ HLL LQ+T P+YY D IIKG + ++ G DT+AVTL+WALS L
Sbjct: 267 DEKREGKEKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAGGTDTTAVTLEWALSSL 326
Query: 328 LNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQGIISETLRLSPPGPLLVPHCASED 387
LNNP VL+KA+ EID IG +R++ E D+ +L YLQ I+SETLRL P P+L+PH AS+D
Sbjct: 327 LNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRLYPAAPMLLPHVASKD 386
Query: 388 CKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKPERHVNAVGFENSYKLLPFGLGRRA 447
CK+GGYD+PR T++L NAWAIHRDP +W+D SFKPER + KL+PFGLGRRA
Sbjct: 387 CKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEKE---GEAKKLMPFGLGRRA 446
Query: 448 CPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMREGEGLTMPKVESLVAKCRPR-FIMK 506
CPG +AQR+V L+L SLIQCFEW+++ VDM EG GLTMPK L A CR R F+ K
Sbjct: 447 CPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKARPLEAMCRARDFVGK 495
BLAST of CsaV3_2G035310 vs. TAIR 10
Match:
AT4G37430.1 (cytochrome P450, family 91, subfamily A, polypeptide 2 )
HSP 1 Score: 486.5 bits (1251), Expect = 2.6e-137
Identity = 243/507 (47.93%), Postives = 354/507 (69.82%), Query Frame = 0
Query: 6 LLYALLSLISLLLGYSFLF-KPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQKYG 65
L + LL L+ L++ Y FL+ K R NLPP P PF+GHLHL+K P+H+ LQ S +YG
Sbjct: 2 LYFILLPLLFLVISYKFLYSKTQRFNLPPGPP-SRPFVGHLHLMKPPIHRLLQRYSNQYG 61
Query: 66 HVFSLKFGSRLVLLVSSPSAVQECFT-KNDIILANRPSLNSGKYLSYNNTTMAVSPYGEH 125
+FSL+FGSR V++++SPS QE FT +NDI+L++RP + KY++YN+TT+ +PYG+H
Sbjct: 62 PIFSLRFGSRRVVVITSPSLAQESFTGQNDIVLSSRPLQLTAKYVAYNHTTVGTAPYGDH 121
Query: 126 WRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDE---FRVVELESMLLD 185
WRNLRRIC+ EI S+ RL +F IR++E+ R+L +L + +E F +ELE +L D
Sbjct: 122 WRNLRRICSQEILSSHRLINFQHIRKDEILRMLTRLSRYTQTSNESNDFTHIELEPLLSD 181
Query: 186 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 245
LT N ++RMV GK++ ++ N E K+LV I ++G+ + D++P+
Sbjct: 182 LTFNNIVRMVTGKRYYGDDVNNKEEAE------LFKKLVYDIAMYSGANHSADYLPILK- 241
Query: 246 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 305
+ + + K + IG+ MD++LQRL+DE R ++ EGNTM+ HL+ LQ+ P+YY+D+IIKG
Sbjct: 242 LFGNKFEKEVKAIGKSMDDILQRLLDECRRDK-EGNTMVNHLISLQQQQPEYYTDVIIKG 301
Query: 306 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 365
L+ +++AG +TSAVTL+WA+++LL NP VL+KA++EID IG++R+++E D++ L YLQ
Sbjct: 302 LMMSMMLAGTETSAVTLEWAMANLLRNPEVLEKARSEIDEKIGKDRLIDESDIAVLPYLQ 361
Query: 366 GIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKP 425
++SET RL P P L+P ++D KIGGYDVPR+TIV++NAWAIHRDP +WE+ F P
Sbjct: 362 NVVSETFRLFPVAPFLIPRSPTDDMKIGGYDVPRDTIVMVNAWAIHRDPEIWEEPEKFNP 421
Query: 426 ERHVNAVGFE-NSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMR 485
+R+ + G + YKL+PFG GRR CPG + QR+V L L SLIQCFEW+ + +DM
Sbjct: 422 DRYNDGCGSDYYVYKLMPFGNGRRTCPGAGLGQRIVTLALGSLIQCFEWENVKGEEMDMS 481
Query: 486 EGEGLTMPKVESLVAKCRPRFIMKVVL 507
E GL M K++ L A CRPR IM +L
Sbjct: 482 ESTGLGMRKMDPLRAMCRPRPIMSKLL 499
BLAST of CsaV3_2G035310 vs. TAIR 10
Match:
AT3G28740.1 (Cytochrome P450 superfamily protein )
HSP 1 Score: 485.7 bits (1249), Expect = 4.5e-137
Identity = 252/507 (49.70%), Postives = 344/507 (67.85%), Query Frame = 0
Query: 7 LYALLSL--ISLLLGYSFLF--KPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQK 66
+Y +LSL + L LF +P +LNLPP+P P IGHLHLLK P+H+ +LS+
Sbjct: 11 IYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSES 70
Query: 67 YGH--VFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 126
+ +FSL GSRLV +VSS + +ECFTKND++LANRP GK++ YN+TTM + Y
Sbjct: 71 LNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAY 130
Query: 127 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD 186
G+ WRNLRRI T+EIFS+ RLNSF IR++E++RL+ L N + F VE++ + +
Sbjct: 131 GDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKN--SQHGFVKVEMKPLFMC 190
Query: 187 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 246
LT N ++RMV GK+F + +N N K +++L+ +++ G+ N D+ P+ +
Sbjct: 191 LTINNIIRMVAGKRFYGDGTENDN------EAKHVRQLIAEVVVSGGAGNAADYFPILRY 250
Query: 247 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 306
+ + Y K + K+ R+DE LQ LV+E R E+ +GNTMI HLL LQ+T P YY+D+IIKG
Sbjct: 251 V--TNYEKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKG 310
Query: 307 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 366
+I +++AG DTSA TL+WA+S+LLN+P VL KAK EID IG +R+V E D+ L YLQ
Sbjct: 311 IILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQ 370
Query: 367 GIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKP 426
I+SETLRL P P+L+PH ASEDC + GYDVPR TI+L+NAWAIHRDP +WE+ FKP
Sbjct: 371 HIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKP 430
Query: 427 ERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMRE 486
ER KL+PFG+GRR+CPG +AQR+V L L SL+QCFEW+++ +DMRE
Sbjct: 431 ERFEKK---GEDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRE 490
Query: 487 GE-GLTMPKVESLVAKCRPRFIMKVVL 507
E G TM K SL A C+ R I+ VL
Sbjct: 491 SEKGTTMRKATSLQAMCKARPIVHKVL 504
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_004151924.2 | 2.7e-301 | 100.00 | cytochrome P450 81Q32 [Cucumis sativus] >KGN63284.1 hypothetical protein Csa_022... | [more] |
KAA0032538.1 | 4.1e-273 | 91.21 | isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa] | [more] |
XP_038902403.1 | 1.0e-244 | 81.32 | cytochrome P450 81Q32-like [Benincasa hispida] | [more] |
XP_038902241.1 | 1.7e-239 | 81.10 | cytochrome P450 81Q32-like [Benincasa hispida] | [more] |
XP_008455822.2 | 2.0e-232 | 82.06 | PREDICTED: LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like [Cucumis melo] | [more] |
Match Name | E-value | Identity | Description | |
W8JMU7 | 1.1e-148 | 52.26 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
Q9FG65 | 3.4e-145 | 52.75 | Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1 | [more] |
Q6WNQ9 | 5.7e-137 | 51.61 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
Q6WNQ8 | 1.3e-136 | 50.50 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
O65790 | 3.7e-136 | 47.93 | Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2 | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0LQI5 | 1.3e-301 | 100.00 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1 | [more] |
A0A5A7SPU0 | 2.0e-273 | 91.21 | Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... | [more] |
A0A1S3C1S2 | 9.9e-233 | 82.06 | LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=L... | [more] |
A0A1S3C323 | 1.6e-222 | 75.25 | cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495924 PE=3 SV=1 | [more] |
A0A5D3BGH3 | 1.2e-209 | 76.61 | Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... | [more] |