CsaV3_2G035140 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_2G035140
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionAPO protein 4, mitochondrial
Locationchr2: 23505457 .. 23506957 (+)
RNA-Seq ExpressionCsaV3_2G035140
SyntenyCsaV3_2G035140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAACTATGGCTTTTATGGCTATTAGAAGGAAGTTCAGGGACAATGTTGTCCAGGAGTTCATGCTACAGCGGTGCTACAGCTCTAAAGTCAACTTGAAGAAACTTCGCCCAATGATTTTAAAGAGAATTCAAGATCGGGCAAAAAACTATCCTATAAAGGGAATGACTCCAGTGGCACAGCAAGTCCTTGAAGCTAGGGCAATGCTTATACATGGAGTTTCCACTCTTCTCAAATCATTTCCAGTTCTCTCTTGCAAGTTAGTGTCCCTGGGCCGGAACAAACTCTCTTCAGTTTTTTTTTCTTTCTTTTTGTTATGATGTTACTCTGCTTCCTTTTCTTTTTCCTTTCCTTTTTCCTTTCTCTTTTTATAGTTTTATATAAATTGTATGAGTGGACTAGTATGTGTATTAGAGTTTTTCCGTTTTGTCATAAATGCTTAGTGGCCTTGATTTTCAGTATGATGTTTCTTGACAAGTAGCCGATATAAGAAATTTCCTGCAGTTGAAGTAAATTGAATGAATTCGACAATATAATCTGGCTCTTTATGCCCCCTAGGACCGTCTGTTAAGTTTGTTTGTTTTTCTTTTTAATTTTAGATTGAACCATCTTAAGGTGTTAATATGAATGCTGCTTCTAGAACTCCTAGACACTTGTGTCAGTCTGTTCTTTCTAAAGTTTACATTCAACAATCTCAACTGTTAGCATGAATACATCTTTGTGAATGTTGTTTCTGATACTCCTAGGTCGTTGTATTGTTTTTGTAGATTTTGTCCAGAAGTATATGTTGGTGAGGAAGGTCATCTAATACGAAGTTGTGGTGGTTATAAACGTGGTGCCAAGAATCAGGTTCATCAGTGGATCAGAGGTGATTTGAAGGATATCATTGTCCCAGTAGAAGCATTTCACCTCCATCACATGTTCCAAGATGTTATTAAACATGACGAGAGGTTCAACTTTGAACGTGTTCCAGCAGTAGTTGAGCTATGCTCCCAAGCTGGGGCCAATCCAGATGATAAAAATCTGGCTTCAAGCACTCAGAATTCAGCTGAAGGTGGTGGCTCAGGCATGGATGAACCTCTGTCAGATCACGAGATGATGCTTTTAGCAACTGAAACCATCAGGGCATGGGAAACACTTCGGACCGGCGTGCAGAAGCTGTTAATGGTATATCCTACTAAAGTCTGTAAGTACTGCTCAGAAGTTCATGTTGGGCCATCAGGACACAAAGCTAGACTTTGTGGAGTTTTTACATATGAGAGTTGGCGAGGGTCCCATTTCTGGGAGAAAGCAGATGTAGATGATTTAGTTCCTCCGAAGATCGTTTGGCATCGGAGACAACAAGATCCTCCTGTGCTTGTTGACAAAGGGAAGGATTATTATGGACACGCACCAGCAGTGGTGGCTCTTTGCACGCAAGCAGGAGTCATTGCACCATTTAAGTATCACTGTATGATGAAAGTTCAAGGCCTATCGCCTAGAGTCAAACTTGAGCTATGA

mRNA sequence

ATGAAAACTATGGCTTTTATGGCTATTAGAAGGAAGTTCAGGGACAATGTTGTCCAGGAGTTCATGCTACAGCGGTGCTACAGCTCTAAAGTCAACTTGAAGAAACTTCGCCCAATGATTTTAAAGAGAATTCAAGATCGGGCAAAAAACTATCCTATAAAGGGAATGACTCCAGTGGCACAGCAAGTCCTTGAAGCTAGGGCAATGCTTATACATGGAGTTTCCACTCTTCTCAAATCATTTCCAGTTCTCTCTTGCAAATTTTGTCCAGAAGTATATGTTGGTGAGGAAGGTCATCTAATACGAAGTTGTGGTGGTTATAAACGTGGTGCCAAGAATCAGGTTCATCAGTGGATCAGAGGTGATTTGAAGGATATCATTGTCCCAGTAGAAGCATTTCACCTCCATCACATGTTCCAAGATGTTATTAAACATGACGAGAGGTTCAACTTTGAACGTGTTCCAGCAGTAGTTGAGCTATGCTCCCAAGCTGGGGCCAATCCAGATGATAAAAATCTGGCTTCAAGCACTCAGAATTCAGCTGAAGGTGGTGGCTCAGGCATGGATGAACCTCTGTCAGATCACGAGATGATGCTTTTAGCAACTGAAACCATCAGGGCATGGGAAACACTTCGGACCGGCGTGCAGAAGCTGTTAATGGTATATCCTACTAAAGTCTGTAAGTACTGCTCAGAAGTTCATGTTGGGCCATCAGGACACAAAGCTAGACTTTGTGGAGTTTTTACATATGAGAGTTGGCGAGGGTCCCATTTCTGGGAGAAAGCAGATGTAGATGATTTAGTTCCTCCGAAGATCGTTTGGCATCGGAGACAACAAGATCCTCCTGTGCTTGTTGACAAAGGGAAGGATTATTATGGACACGCACCAGCAGTGGTGGCTCTTTGCACGCAAGCAGGAGTCATTGCACCATTTAAGTATCACTGTATGATGAAAGTTCAAGGCCTATCGCCTAGAGTCAAACTTGAGCTATGA

Coding sequence (CDS)

ATGAAAACTATGGCTTTTATGGCTATTAGAAGGAAGTTCAGGGACAATGTTGTCCAGGAGTTCATGCTACAGCGGTGCTACAGCTCTAAAGTCAACTTGAAGAAACTTCGCCCAATGATTTTAAAGAGAATTCAAGATCGGGCAAAAAACTATCCTATAAAGGGAATGACTCCAGTGGCACAGCAAGTCCTTGAAGCTAGGGCAATGCTTATACATGGAGTTTCCACTCTTCTCAAATCATTTCCAGTTCTCTCTTGCAAATTTTGTCCAGAAGTATATGTTGGTGAGGAAGGTCATCTAATACGAAGTTGTGGTGGTTATAAACGTGGTGCCAAGAATCAGGTTCATCAGTGGATCAGAGGTGATTTGAAGGATATCATTGTCCCAGTAGAAGCATTTCACCTCCATCACATGTTCCAAGATGTTATTAAACATGACGAGAGGTTCAACTTTGAACGTGTTCCAGCAGTAGTTGAGCTATGCTCCCAAGCTGGGGCCAATCCAGATGATAAAAATCTGGCTTCAAGCACTCAGAATTCAGCTGAAGGTGGTGGCTCAGGCATGGATGAACCTCTGTCAGATCACGAGATGATGCTTTTAGCAACTGAAACCATCAGGGCATGGGAAACACTTCGGACCGGCGTGCAGAAGCTGTTAATGGTATATCCTACTAAAGTCTGTAAGTACTGCTCAGAAGTTCATGTTGGGCCATCAGGACACAAAGCTAGACTTTGTGGAGTTTTTACATATGAGAGTTGGCGAGGGTCCCATTTCTGGGAGAAAGCAGATGTAGATGATTTAGTTCCTCCGAAGATCGTTTGGCATCGGAGACAACAAGATCCTCCTGTGCTTGTTGACAAAGGGAAGGATTATTATGGACACGCACCAGCAGTGGTGGCTCTTTGCACGCAAGCAGGAGTCATTGCACCATTTAAGTATCACTGTATGATGAAAGTTCAAGGCCTATCGCCTAGAGTCAAACTTGAGCTATGA

Protein sequence

MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVAQQVLEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIRGDLKDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNSAEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGHKARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVALCTQAGVIAPFKYHCMMKVQGLSPRVKLEL*
Homology
BLAST of CsaV3_2G035140 vs. NCBI nr
Match: XP_004151879.1 (APO protein 4, mitochondrial [Cucumis sativus] >XP_031737269.1 APO protein 4, mitochondrial [Cucumis sativus] >XP_031737270.1 APO protein 4, mitochondrial [Cucumis sativus] >KGN63269.1 hypothetical protein Csa_022178 [Cucumis sativus])

HSP 1 Score: 684.5 bits (1765), Expect = 4.6e-193
Identity = 330/330 (100.00%), Postives = 330/330 (100.00%), Query Frame = 0

Query: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60
           MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60

Query: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIR 120
           QQVLEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIR
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 121 GDLKDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNS 180
           GDLKDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNS
Sbjct: 121 GDLKDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNS 180

Query: 181 AEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240
           AEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH
Sbjct: 181 AEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240

Query: 241 KARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVA 300
           KARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVA
Sbjct: 241 KARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVA 300

Query: 301 LCTQAGVIAPFKYHCMMKVQGLSPRVKLEL 331
           LCTQAGVIAPFKYHCMMKVQGLSPRVKLEL
Sbjct: 301 LCTQAGVIAPFKYHCMMKVQGLSPRVKLEL 330

BLAST of CsaV3_2G035140 vs. NCBI nr
Match: KAA0043413.1 (APO protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 661.4 bits (1705), Expect = 4.2e-186
Identity = 317/328 (96.65%), Postives = 320/328 (97.56%), Query Frame = 0

Query: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60
           MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60

Query: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIR 120
           QQVLEARAMLIHGVSTLLKSFPVLSCK+CPEVYVG EGHLIRSCGGYKRGAKNQVHQWIR
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKYCPEVYVGAEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 121 GDLKDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNS 180
           GDL DIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELC QAG NPDDK+LASSTQNS
Sbjct: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCCQAGVNPDDKDLASSTQNS 180

Query: 181 AEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240
           AEGGGSGMDEP SDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH
Sbjct: 181 AEGGGSGMDEPFSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240

Query: 241 KARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVA 300
           KARLCGVF YESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKG+DYYGHAPAVVA
Sbjct: 241 KARLCGVFKYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGRDYYGHAPAVVA 300

Query: 301 LCTQAGVIAPFKYHCMMKVQGLSPRVKL 329
           LCTQAG IAPFKYHCMMKVQGLSPRV L
Sbjct: 301 LCTQAGAIAPFKYHCMMKVQGLSPRVNL 328

BLAST of CsaV3_2G035140 vs. NCBI nr
Match: XP_008455841.1 (PREDICTED: APO protein 4, mitochondrial [Cucumis melo] >XP_008455842.1 PREDICTED: APO protein 4, mitochondrial [Cucumis melo] >TYJ98297.1 APO protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 657.1 bits (1694), Expect = 7.8e-185
Identity = 315/328 (96.04%), Postives = 319/328 (97.26%), Query Frame = 0

Query: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60
           MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAK+YPIKGMTPVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKSYPIKGMTPVA 60

Query: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIR 120
           QQVLEARAMLIHGVSTLLKSFPVLSCK+CPEVYVG EGHLIRSCGGYKRGAKNQVHQWIR
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKYCPEVYVGAEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 121 GDLKDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNS 180
           GDL DIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELC QAG NPDDK+LASSTQNS
Sbjct: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCCQAGVNPDDKDLASSTQNS 180

Query: 181 AEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240
           AEGGGSGMDEP SDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH
Sbjct: 181 AEGGGSGMDEPFSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240

Query: 241 KARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVA 300
           KARLCGVF YESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKG+DYYGHAPAVVA
Sbjct: 241 KARLCGVFKYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGRDYYGHAPAVVA 300

Query: 301 LCTQAGVIAPFKYHCMMKVQGLSPRVKL 329
           LC QAG IAPFKYHCMMKVQGLSPRV L
Sbjct: 301 LCMQAGAIAPFKYHCMMKVQGLSPRVNL 328

BLAST of CsaV3_2G035140 vs. NCBI nr
Match: XP_038901944.1 (APO protein 4, mitochondrial [Benincasa hispida] >XP_038901945.1 APO protein 4, mitochondrial [Benincasa hispida] >XP_038901946.1 APO protein 4, mitochondrial [Benincasa hispida] >XP_038901947.1 APO protein 4, mitochondrial [Benincasa hispida])

HSP 1 Score: 615.5 bits (1586), Expect = 2.6e-172
Identity = 288/330 (87.27%), Postives = 312/330 (94.55%), Query Frame = 0

Query: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60
           MKTMAFMA++RKFR+ VVQEF+L RCYSSKVNLKKLRPMILKRIQ+R KNYPI+GMTPVA
Sbjct: 1   MKTMAFMALKRKFREIVVQEFVLHRCYSSKVNLKKLRPMILKRIQNREKNYPIRGMTPVA 60

Query: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIR 120
           QQVLEAR MLIHGVSTLLKSFPVLSCKFCPEVYVGE+GHLIRSCGGYKRGAKNQVH+WIR
Sbjct: 61  QQVLEARTMLIHGVSTLLKSFPVLSCKFCPEVYVGEKGHLIRSCGGYKRGAKNQVHEWIR 120

Query: 121 GDLKDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNS 180
           GDL DIIVPVE+FHLHHMFQDVIKHDERFNFERVPAVVELC QAGANP+D+N +SST+NS
Sbjct: 121 GDLNDIIVPVESFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENQSSSTRNS 180

Query: 181 AEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240
            EGGGSG D+PLS HEMMLLATETIRAWETLRTGVQKLLMVYP KVCKYCSEVHVGPSGH
Sbjct: 181 GEGGGSGRDKPLSGHEMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGH 240

Query: 241 KARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVA 300
           KARLCGVF YE WRGSHFWEKAD+DDLVPPK+VWHRR QDPPVL+D+G+DYYGH PAV+A
Sbjct: 241 KARLCGVFKYEHWRGSHFWEKADIDDLVPPKLVWHRRPQDPPVLIDEGRDYYGHTPAVLA 300

Query: 301 LCTQAGVIAPFKYHCMMKVQGLSPRVKLEL 331
           LCTQAG +AP KYHCMMKVQG+SPR +L+L
Sbjct: 301 LCTQAGALAPSKYHCMMKVQGISPRSQLKL 330

BLAST of CsaV3_2G035140 vs. NCBI nr
Match: XP_023532078.1 (APO protein 4, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 572.0 bits (1473), Expect = 3.3e-159
Identity = 271/327 (82.87%), Postives = 300/327 (91.74%), Query Frame = 0

Query: 4   MAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVAQQV 63
           MAFMA+RRK  +NVVQEFMLQRCYSSKV+LKKLRPMILKR+Q+RA NYPIKGM PVAQ+V
Sbjct: 1   MAFMALRRKLGENVVQEFMLQRCYSSKVDLKKLRPMILKRLQNRANNYPIKGMIPVAQEV 60

Query: 64  LEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIRGDL 123
           LEARA LIHGVSTLLK+FPVLSCKFCPEVYVG++GHLIRSCGGYK GAKN+VH+WIRGDL
Sbjct: 61  LEARAKLIHGVSTLLKTFPVLSCKFCPEVYVGDKGHLIRSCGGYKHGAKNRVHEWIRGDL 120

Query: 124 KDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNS-AE 183
            DI+VPVE+FHL HMFQDVIKHDERF+F+RVPAVVELC QAGANP+D+NL+SST NS  +
Sbjct: 121 NDILVPVESFHLRHMFQDVIKHDERFDFDRVPAVVELCWQAGANPNDENLSSSTWNSDGD 180

Query: 184 GGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGHKA 243
           G G+G DE LS HE+M+LA  TIRAWET+RTGVQKLLMVYPTKVCK+CSEVHVGPSGHKA
Sbjct: 181 GNGNGRDEHLSSHELMVLANTTIRAWETVRTGVQKLLMVYPTKVCKHCSEVHVGPSGHKA 240

Query: 244 RLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVALC 303
           RLCGVF YESWRGSHFWEKADVDDLVPPK VWHRR QDPPVLVD+G+DYYGHAPAV+ALC
Sbjct: 241 RLCGVFKYESWRGSHFWEKADVDDLVPPKSVWHRRAQDPPVLVDEGRDYYGHAPAVLALC 300

Query: 304 TQAGVIAPFKYHCMMKVQGLSPRVKLE 330
            QAG IAP KYHCMMKVQGLS   +L+
Sbjct: 301 LQAGAIAPSKYHCMMKVQGLSHHSQLK 327

BLAST of CsaV3_2G035140 vs. ExPASy Swiss-Prot
Match: Q9LSZ0 (APO protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO4 PE=2 SV=2)

HSP 1 Score: 367.1 bits (941), Expect = 2.1e-100
Identity = 168/292 (57.53%), Postives = 220/292 (75.34%), Query Frame = 0

Query: 32  NLKKLRPMILKRIQDRAKNYPIKGMTPVAQQVLEARAMLIHGVSTLLKSFPVLSCKFCPE 91
           +L+KLRPMILKRI++RAK+YP+K + PVA+++L AR  LI  ++ LLK FPVL+CKFC E
Sbjct: 34  DLRKLRPMILKRIENRAKDYPVKEIVPVAEEILIARKNLISNIAALLKVFPVLTCKFCSE 93

Query: 92  VYVGEEGHLIRSCGGYKRGAKNQVHQWIRGDLKDIIVPVEAFHLHHMFQDVIKHDERFNF 151
           V+VG+EGHLI +C  Y R   N++H+W+ G + DI+VPVE++HLH++ Q VI+H ERF++
Sbjct: 94  VFVGKEGHLIETCRSYIRRGNNRLHEWVPGSINDILVPVESYHLHNISQGVIRHQERFDY 153

Query: 152 ERVPAVVELCSQAGANPDDKNLASSTQNSAEGGGSGMDEPLSDHEMMLLATETIRAWETL 211
           +RVPA++ELC QAGA   ++ L  S  +            L   ++  +    + AWE +
Sbjct: 154 DRVPAILELCCQAGAIHPEEILQYSEIHDNPQISEEDIRSLPAGDLKYVGANALMAWEKV 213

Query: 212 RTGVQKLLMVYPTKVCKYCSEVHVGPSGHKARLCGVFTYESWRGSHFWEKADVDDLVPPK 271
           R GV+KLL+VYP+KVCK C EVHVGPSGHKARLCGVF YESWRG+H+WEKA V+DLVP K
Sbjct: 214 RAGVKKLLLVYPSKVCKRCKEVHVGPSGHKARLCGVFKYESWRGTHYWEKAGVNDLVPEK 273

Query: 272 IVWHRRQQDPPVLVDKGKDYYGHAPAVVALCTQAGVIAPFKYHCMMKVQGLS 324
           +VWHRR QDP VLVD+G+ YYGHAPA+V+LC+  G I P KY C MK QGLS
Sbjct: 274 MVWHRRPQDPVVLVDEGRSYYGHAPAIVSLCSHTGAIVPVKYACKMKPQGLS 325

BLAST of CsaV3_2G035140 vs. ExPASy Swiss-Prot
Match: Q8W4A5 (APO protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO2 PE=2 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.0e-57
Identity = 123/336 (36.61%), Postives = 178/336 (52.98%), Query Frame = 0

Query: 23  LQRCYSSKVNLKKLRPMILKRIQDRAKN-YPIKGMTPVAQQVLEARAMLIHGVSTLLKSF 82
           L+R    +V   K +P   KR     KN   +K + P+A +V  AR  LI+ +  L+K  
Sbjct: 102 LRRAARERVKNNKDKP---KRPLPPPKNGMVVKSLVPLAYKVYNARIRLINNLHRLMKVV 161

Query: 83  PVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIRGDLKDIIVPVEAFHLHHMFQD 142
            V +C +C E++VG  GH  +SC G     +  +H+W    ++D+IVP+EA+HL      
Sbjct: 162 RVNACGWCNEIHVGPYGHPFKSCKGPNTSQRKGLHEWTNSVIEDVIVPLEAYHLFDRLGK 221

Query: 143 VIKHDERFNFERVPAVVELCSQAGAN-----------------------------PDDKN 202
            I+HDERF+  RVPAVVELC Q G                               PD + 
Sbjct: 222 RIRHDERFSIPRVPAVVELCIQGGVEIPEFPAKRRRKPIIRIGKSEFVDADETELPDPEP 281

Query: 203 LASSTQNSAEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSE 262
                    E   S +  P S+ E + LA ET++AWE +R G +KL+ +Y  +VC YC E
Sbjct: 282 QPPPVPLLTELPVSEITPPSSEEETVSLAEETLQAWEEMRAGAKKLMRMYRVRVCGYCPE 341

Query: 263 VHVGPSGHKARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYY 322
           VHVGP+GHKA+ CG F ++   G H W+ A +DDL+PP+ VWH    + P +  + + +Y
Sbjct: 342 VHVGPTGHKAQNCGAFKHQQRNGQHGWQSAVLDDLIPPRYVWHVPDVNGPPMQRELRSFY 401

Query: 323 GHAPAVVALCTQAGVIAPFKYHCMMKVQ-GLSPRVK 328
           G APAVV +C QAG + P  Y   M+++ G+   VK
Sbjct: 402 GQAPAVVEICAQAGAVVPEHYRATMRLEVGIPSSVK 434

BLAST of CsaV3_2G035140 vs. ExPASy Swiss-Prot
Match: Q9XIR4 (APO protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO1 PE=2 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 9.7e-53
Identity = 114/325 (35.08%), Postives = 169/325 (52.00%), Query Frame = 0

Query: 34  KKLRPMILKRIQDRAKN-YPIKGMTPVAQQVLEARAMLIHGVSTLLKSFPVLSCKFCPEV 93
           KKL  M +++  D  KN   +  + PVA QV++   +LI G++ LL   PV +C  C  V
Sbjct: 103 KKLAQMGIEKQLDPPKNGLLVPNLVPVADQVIDNWKLLIKGLAQLLHVVPVFACSECGAV 162

Query: 94  YVGEEGHLIRSCGGYKRGAKNQVHQWIRGDLKDIIVPVEAFHLHHMFQDVIKHDERFNFE 153
           +V   GH IR C G     +   H W++G + D+++PVE++H++  F   IKH+ RF +E
Sbjct: 163 HVANVGHNIRDCNGPTNSQRRGSHSWVKGTINDVLIPVESYHMYDPFGRRIKHETRFEYE 222

Query: 154 RVPAVVELCSQAGANPDDKNLASSTQ-------NSAEGGG-----------SGMDEPLSD 213
           R+PA+VELC QAG    +      TQ          + GG           S +  PL++
Sbjct: 223 RIPALVELCIQAGVEIPEYPCRRRTQPIRMMGKRVIDRGGYHKEPEKPQTSSSLSSPLAE 282

Query: 214 ---------------HEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSG 273
                           ++  +A ET+ A+E +R GV KL+  +  K C YCSEVHVGP G
Sbjct: 283 LDTLGVFERYPPPTPEDIPKIAQETMDAYEKVRLGVTKLMRKFTVKACGYCSEVHVGPWG 342

Query: 274 HKARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVV 325
           H  +LCG F ++   G H W+ A VD++ PP  VWH R      L    + +YG APA+V
Sbjct: 343 HSVKLCGEFKHQWRDGKHGWQDALVDEVFPPNYVWHVRDLKGNPLTGNLRRFYGKAPALV 402

BLAST of CsaV3_2G035140 vs. ExPASy Swiss-Prot
Match: Q9FH50 (APO protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO3 PE=2 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.3e-44
Identity = 110/332 (33.13%), Postives = 174/332 (52.41%), Query Frame = 0

Query: 22  MLQRCYSSKVNLKKLRPMILKRIQDRAKN-YPIKGMTPVAQQVLEARAMLIHGVSTLLKS 81
           +++R    K  L+KL+P   + ++D   N   +  +  VA  V   R ML+ G+S ++  
Sbjct: 66  LIRRAKEEK-QLRKLQP--CRVLEDPPDNGLLVPELVDVAHCVHRCRNMLLSGLSKIIHH 125

Query: 82  FPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIRGDLKDIIVPVEAFHLH-HMF 141
            PV  C+ C EV++G++GH IR+C G   G+++  H W RG + D+++  + FHL+    
Sbjct: 126 VPVHRCRLCAEVHIGKQGHEIRTCTGPGSGSRSATHVWKRGRVSDVVLFPKCFHLYDRAV 185

Query: 142 QDVIKHDERFNFERVPAVVELCSQAGANPD--DKNLASSTQNSAEG----------GGSG 201
           +  + HDERF   ++ AV+ELC QAG + +       S    S EG          G S 
Sbjct: 186 KPRVIHDERFTVPKISAVLELCIQAGVDLEKFPSKRRSKPVYSIEGRIVDFEDVNDGNSE 245

Query: 202 M-----------DEPLSDHEMML--LATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVH 261
           +           D+   + +  L  L+ ET+ +W  +  GV+KL+  Y    C YC E+ 
Sbjct: 246 LAVTSTTTLIQEDDRCKEEKKSLKELSFETMESWFEMVLGVRKLMERYRVWTCGYCPEIQ 305

Query: 262 VGPSGHKARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWH-RRQQDPPVLVDKGKDYYG 321
           VGP GHK R+C    ++   G H W++A +DD+V P  VWH R   D  VL +  K +YG
Sbjct: 306 VGPKGHKVRMCKATKHQMRDGMHAWQEATIDDVVGPTYVWHVRDPTDGSVLDNSLKRFYG 365

Query: 322 HAPAVVALCTQAGVIAPFKYHCMMKVQGLSPR 326
            APAV+ +C Q G   P +Y+ MM++  + P+
Sbjct: 366 KAPAVIEMCVQGGAPVPDQYNSMMRLDVVYPQ 394

BLAST of CsaV3_2G035140 vs. ExPASy TrEMBL
Match: A0A0A0LQG5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G421010 PE=4 SV=1)

HSP 1 Score: 684.5 bits (1765), Expect = 2.2e-193
Identity = 330/330 (100.00%), Postives = 330/330 (100.00%), Query Frame = 0

Query: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60
           MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60

Query: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIR 120
           QQVLEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIR
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 121 GDLKDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNS 180
           GDLKDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNS
Sbjct: 121 GDLKDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNS 180

Query: 181 AEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240
           AEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH
Sbjct: 181 AEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240

Query: 241 KARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVA 300
           KARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVA
Sbjct: 241 KARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVA 300

Query: 301 LCTQAGVIAPFKYHCMMKVQGLSPRVKLEL 331
           LCTQAGVIAPFKYHCMMKVQGLSPRVKLEL
Sbjct: 301 LCTQAGVIAPFKYHCMMKVQGLSPRVKLEL 330

BLAST of CsaV3_2G035140 vs. ExPASy TrEMBL
Match: A0A5A7TJ18 (APO protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1639G00180 PE=4 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 2.0e-186
Identity = 317/328 (96.65%), Postives = 320/328 (97.56%), Query Frame = 0

Query: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60
           MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60

Query: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIR 120
           QQVLEARAMLIHGVSTLLKSFPVLSCK+CPEVYVG EGHLIRSCGGYKRGAKNQVHQWIR
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKYCPEVYVGAEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 121 GDLKDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNS 180
           GDL DIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELC QAG NPDDK+LASSTQNS
Sbjct: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCCQAGVNPDDKDLASSTQNS 180

Query: 181 AEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240
           AEGGGSGMDEP SDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH
Sbjct: 181 AEGGGSGMDEPFSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240

Query: 241 KARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVA 300
           KARLCGVF YESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKG+DYYGHAPAVVA
Sbjct: 241 KARLCGVFKYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGRDYYGHAPAVVA 300

Query: 301 LCTQAGVIAPFKYHCMMKVQGLSPRVKL 329
           LCTQAG IAPFKYHCMMKVQGLSPRV L
Sbjct: 301 LCTQAGAIAPFKYHCMMKVQGLSPRVNL 328

BLAST of CsaV3_2G035140 vs. ExPASy TrEMBL
Match: A0A1S3C343 (APO protein 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495942 PE=4 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 3.8e-185
Identity = 315/328 (96.04%), Postives = 319/328 (97.26%), Query Frame = 0

Query: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60
           MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAK+YPIKGMTPVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKSYPIKGMTPVA 60

Query: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIR 120
           QQVLEARAMLIHGVSTLLKSFPVLSCK+CPEVYVG EGHLIRSCGGYKRGAKNQVHQWIR
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKYCPEVYVGAEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 121 GDLKDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNS 180
           GDL DIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELC QAG NPDDK+LASSTQNS
Sbjct: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCCQAGVNPDDKDLASSTQNS 180

Query: 181 AEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240
           AEGGGSGMDEP SDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH
Sbjct: 181 AEGGGSGMDEPFSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240

Query: 241 KARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVA 300
           KARLCGVF YESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKG+DYYGHAPAVVA
Sbjct: 241 KARLCGVFKYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGRDYYGHAPAVVA 300

Query: 301 LCTQAGVIAPFKYHCMMKVQGLSPRVKL 329
           LC QAG IAPFKYHCMMKVQGLSPRV L
Sbjct: 301 LCMQAGAIAPFKYHCMMKVQGLSPRVNL 328

BLAST of CsaV3_2G035140 vs. ExPASy TrEMBL
Match: A0A5D3BGQ1 (APO protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold232G00100 PE=4 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 3.8e-185
Identity = 315/328 (96.04%), Postives = 319/328 (97.26%), Query Frame = 0

Query: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60
           MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAK+YPIKGMTPVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKSYPIKGMTPVA 60

Query: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIR 120
           QQVLEARAMLIHGVSTLLKSFPVLSCK+CPEVYVG EGHLIRSCGGYKRGAKNQVHQWIR
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKYCPEVYVGAEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 121 GDLKDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNS 180
           GDL DIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELC QAG NPDDK+LASSTQNS
Sbjct: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCCQAGVNPDDKDLASSTQNS 180

Query: 181 AEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240
           AEGGGSGMDEP SDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH
Sbjct: 181 AEGGGSGMDEPFSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240

Query: 241 KARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVA 300
           KARLCGVF YESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKG+DYYGHAPAVVA
Sbjct: 241 KARLCGVFKYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGRDYYGHAPAVVA 300

Query: 301 LCTQAGVIAPFKYHCMMKVQGLSPRVKL 329
           LC QAG IAPFKYHCMMKVQGLSPRV L
Sbjct: 301 LCMQAGAIAPFKYHCMMKVQGLSPRVNL 328

BLAST of CsaV3_2G035140 vs. ExPASy TrEMBL
Match: A0A6J1I7G7 (APO protein 4, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111469835 PE=4 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 1.6e-159
Identity = 272/326 (83.44%), Postives = 298/326 (91.41%), Query Frame = 0

Query: 4   MAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVAQQV 63
           MAFMA+RRK  +NVVQEFMLQRCYSSKV+LKKLRPMILKR+Q+RA NYPIKGM PVAQ+V
Sbjct: 1   MAFMALRRKLGENVVQEFMLQRCYSSKVDLKKLRPMILKRLQNRANNYPIKGMIPVAQEV 60

Query: 64  LEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIRGDL 123
           LEARAMLIHGVSTLLK+FPVLSCKFCPEVYVG++GHLIRSCGGYKRGAKN+VH+WIRGDL
Sbjct: 61  LEARAMLIHGVSTLLKTFPVLSCKFCPEVYVGDKGHLIRSCGGYKRGAKNRVHEWIRGDL 120

Query: 124 KDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNSAEG 183
            DI+VPVE+FHL HMFQDVIKHDERF+FERVPAVVELC QAGANP+D+NL+SST NS   
Sbjct: 121 NDILVPVESFHLRHMFQDVIKHDERFDFERVPAVVELCWQAGANPNDENLSSSTWNS--- 180

Query: 184 GGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGHKAR 243
            G G DE LS HE+M+LA  TIRAWET+RTGVQKLLMVYPTKVCK+CSEVHVGPSGHKAR
Sbjct: 181 DGDGRDEHLSSHELMVLANSTIRAWETVRTGVQKLLMVYPTKVCKHCSEVHVGPSGHKAR 240

Query: 244 LCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVALCT 303
           LCGVF YESWRGSHFWEK DVDDLVPPK VWHRR QDPPVLVD+G+DYYGHAPAV+ALC 
Sbjct: 241 LCGVFKYESWRGSHFWEKGDVDDLVPPKSVWHRRAQDPPVLVDEGRDYYGHAPAVLALCI 300

Query: 304 QAGVIAPFKYHCMMKVQGLSPRVKLE 330
           QAG IAP KYHCMMKVQGLS   +L+
Sbjct: 301 QAGAIAPSKYHCMMKVQGLSHHSQLK 323

BLAST of CsaV3_2G035140 vs. TAIR 10
Match: AT3G21740.1 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 367.1 bits (941), Expect = 1.5e-101
Identity = 168/292 (57.53%), Postives = 220/292 (75.34%), Query Frame = 0

Query: 32  NLKKLRPMILKRIQDRAKNYPIKGMTPVAQQVLEARAMLIHGVSTLLKSFPVLSCKFCPE 91
           +L+KLRPMILKRI++RAK+YP+K + PVA+++L AR  LI  ++ LLK FPVL+CKFC E
Sbjct: 34  DLRKLRPMILKRIENRAKDYPVKEIVPVAEEILIARKNLISNIAALLKVFPVLTCKFCSE 93

Query: 92  VYVGEEGHLIRSCGGYKRGAKNQVHQWIRGDLKDIIVPVEAFHLHHMFQDVIKHDERFNF 151
           V+VG+EGHLI +C  Y R   N++H+W+ G + DI+VPVE++HLH++ Q VI+H ERF++
Sbjct: 94  VFVGKEGHLIETCRSYIRRGNNRLHEWVPGSINDILVPVESYHLHNISQGVIRHQERFDY 153

Query: 152 ERVPAVVELCSQAGANPDDKNLASSTQNSAEGGGSGMDEPLSDHEMMLLATETIRAWETL 211
           +RVPA++ELC QAGA   ++ L  S  +            L   ++  +    + AWE +
Sbjct: 154 DRVPAILELCCQAGAIHPEEILQYSEIHDNPQISEEDIRSLPAGDLKYVGANALMAWEKV 213

Query: 212 RTGVQKLLMVYPTKVCKYCSEVHVGPSGHKARLCGVFTYESWRGSHFWEKADVDDLVPPK 271
           R GV+KLL+VYP+KVCK C EVHVGPSGHKARLCGVF YESWRG+H+WEKA V+DLVP K
Sbjct: 214 RAGVKKLLLVYPSKVCKRCKEVHVGPSGHKARLCGVFKYESWRGTHYWEKAGVNDLVPEK 273

Query: 272 IVWHRRQQDPPVLVDKGKDYYGHAPAVVALCTQAGVIAPFKYHCMMKVQGLS 324
           +VWHRR QDP VLVD+G+ YYGHAPA+V+LC+  G I P KY C MK QGLS
Sbjct: 274 MVWHRRPQDPVVLVDEGRSYYGHAPAIVSLCSHTGAIVPVKYACKMKPQGLS 325

BLAST of CsaV3_2G035140 vs. TAIR 10
Match: AT5G57930.1 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 225.3 bits (573), Expect = 7.1e-59
Identity = 123/336 (36.61%), Postives = 178/336 (52.98%), Query Frame = 0

Query: 23  LQRCYSSKVNLKKLRPMILKRIQDRAKN-YPIKGMTPVAQQVLEARAMLIHGVSTLLKSF 82
           L+R    +V   K +P   KR     KN   +K + P+A +V  AR  LI+ +  L+K  
Sbjct: 102 LRRAARERVKNNKDKP---KRPLPPPKNGMVVKSLVPLAYKVYNARIRLINNLHRLMKVV 161

Query: 83  PVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIRGDLKDIIVPVEAFHLHHMFQD 142
            V +C +C E++VG  GH  +SC G     +  +H+W    ++D+IVP+EA+HL      
Sbjct: 162 RVNACGWCNEIHVGPYGHPFKSCKGPNTSQRKGLHEWTNSVIEDVIVPLEAYHLFDRLGK 221

Query: 143 VIKHDERFNFERVPAVVELCSQAGAN-----------------------------PDDKN 202
            I+HDERF+  RVPAVVELC Q G                               PD + 
Sbjct: 222 RIRHDERFSIPRVPAVVELCIQGGVEIPEFPAKRRRKPIIRIGKSEFVDADETELPDPEP 281

Query: 203 LASSTQNSAEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSE 262
                    E   S +  P S+ E + LA ET++AWE +R G +KL+ +Y  +VC YC E
Sbjct: 282 QPPPVPLLTELPVSEITPPSSEEETVSLAEETLQAWEEMRAGAKKLMRMYRVRVCGYCPE 341

Query: 263 VHVGPSGHKARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYY 322
           VHVGP+GHKA+ CG F ++   G H W+ A +DDL+PP+ VWH    + P +  + + +Y
Sbjct: 342 VHVGPTGHKAQNCGAFKHQQRNGQHGWQSAVLDDLIPPRYVWHVPDVNGPPMQRELRSFY 401

Query: 323 GHAPAVVALCTQAGVIAPFKYHCMMKVQ-GLSPRVK 328
           G APAVV +C QAG + P  Y   M+++ G+   VK
Sbjct: 402 GQAPAVVEICAQAGAVVPEHYRATMRLEVGIPSSVK 434

BLAST of CsaV3_2G035140 vs. TAIR 10
Match: AT5G57930.2 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 225.3 bits (573), Expect = 7.1e-59
Identity = 123/336 (36.61%), Postives = 178/336 (52.98%), Query Frame = 0

Query: 23  LQRCYSSKVNLKKLRPMILKRIQDRAKN-YPIKGMTPVAQQVLEARAMLIHGVSTLLKSF 82
           L+R    +V   K +P   KR     KN   +K + P+A +V  AR  LI+ +  L+K  
Sbjct: 105 LRRAARERVKNNKDKP---KRPLPPPKNGMVVKSLVPLAYKVYNARIRLINNLHRLMKVV 164

Query: 83  PVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIRGDLKDIIVPVEAFHLHHMFQD 142
            V +C +C E++VG  GH  +SC G     +  +H+W    ++D+IVP+EA+HL      
Sbjct: 165 RVNACGWCNEIHVGPYGHPFKSCKGPNTSQRKGLHEWTNSVIEDVIVPLEAYHLFDRLGK 224

Query: 143 VIKHDERFNFERVPAVVELCSQAGAN-----------------------------PDDKN 202
            I+HDERF+  RVPAVVELC Q G                               PD + 
Sbjct: 225 RIRHDERFSIPRVPAVVELCIQGGVEIPEFPAKRRRKPIIRIGKSEFVDADETELPDPEP 284

Query: 203 LASSTQNSAEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSE 262
                    E   S +  P S+ E + LA ET++AWE +R G +KL+ +Y  +VC YC E
Sbjct: 285 QPPPVPLLTELPVSEITPPSSEEETVSLAEETLQAWEEMRAGAKKLMRMYRVRVCGYCPE 344

Query: 263 VHVGPSGHKARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYY 322
           VHVGP+GHKA+ CG F ++   G H W+ A +DDL+PP+ VWH    + P +  + + +Y
Sbjct: 345 VHVGPTGHKAQNCGAFKHQQRNGQHGWQSAVLDDLIPPRYVWHVPDVNGPPMQRELRSFY 404

Query: 323 GHAPAVVALCTQAGVIAPFKYHCMMKVQ-GLSPRVK 328
           G APAVV +C QAG + P  Y   M+++ G+   VK
Sbjct: 405 GQAPAVVEICAQAGAVVPEHYRATMRLEVGIPSSVK 437

BLAST of CsaV3_2G035140 vs. TAIR 10
Match: AT1G64810.1 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 208.8 bits (530), Expect = 6.9e-54
Identity = 114/325 (35.08%), Postives = 169/325 (52.00%), Query Frame = 0

Query: 34  KKLRPMILKRIQDRAKN-YPIKGMTPVAQQVLEARAMLIHGVSTLLKSFPVLSCKFCPEV 93
           KKL  M +++  D  KN   +  + PVA QV++   +LI G++ LL   PV +C  C  V
Sbjct: 103 KKLAQMGIEKQLDPPKNGLLVPNLVPVADQVIDNWKLLIKGLAQLLHVVPVFACSECGAV 162

Query: 94  YVGEEGHLIRSCGGYKRGAKNQVHQWIRGDLKDIIVPVEAFHLHHMFQDVIKHDERFNFE 153
           +V   GH IR C G     +   H W++G + D+++PVE++H++  F   IKH+ RF +E
Sbjct: 163 HVANVGHNIRDCNGPTNSQRRGSHSWVKGTINDVLIPVESYHMYDPFGRRIKHETRFEYE 222

Query: 154 RVPAVVELCSQAGANPDDKNLASSTQ-------NSAEGGG-----------SGMDEPLSD 213
           R+PA+VELC QAG    +      TQ          + GG           S +  PL++
Sbjct: 223 RIPALVELCIQAGVEIPEYPCRRRTQPIRMMGKRVIDRGGYHKEPEKPQTSSSLSSPLAE 282

Query: 214 ---------------HEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSG 273
                           ++  +A ET+ A+E +R GV KL+  +  K C YCSEVHVGP G
Sbjct: 283 LDTLGVFERYPPPTPEDIPKIAQETMDAYEKVRLGVTKLMRKFTVKACGYCSEVHVGPWG 342

Query: 274 HKARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVV 325
           H  +LCG F ++   G H W+ A VD++ PP  VWH R      L    + +YG APA+V
Sbjct: 343 HSVKLCGEFKHQWRDGKHGWQDALVDEVFPPNYVWHVRDLKGNPLTGNLRRFYGKAPALV 402

BLAST of CsaV3_2G035140 vs. TAIR 10
Match: AT1G64810.2 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 208.8 bits (530), Expect = 6.9e-54
Identity = 114/325 (35.08%), Postives = 169/325 (52.00%), Query Frame = 0

Query: 34  KKLRPMILKRIQDRAKN-YPIKGMTPVAQQVLEARAMLIHGVSTLLKSFPVLSCKFCPEV 93
           KKL  M +++  D  KN   +  + PVA QV++   +LI G++ LL   PV +C  C  V
Sbjct: 127 KKLAQMGIEKQLDPPKNGLLVPNLVPVADQVIDNWKLLIKGLAQLLHVVPVFACSECGAV 186

Query: 94  YVGEEGHLIRSCGGYKRGAKNQVHQWIRGDLKDIIVPVEAFHLHHMFQDVIKHDERFNFE 153
           +V   GH IR C G     +   H W++G + D+++PVE++H++  F   IKH+ RF +E
Sbjct: 187 HVANVGHNIRDCNGPTNSQRRGSHSWVKGTINDVLIPVESYHMYDPFGRRIKHETRFEYE 246

Query: 154 RVPAVVELCSQAGANPDDKNLASSTQ-------NSAEGGG-----------SGMDEPLSD 213
           R+PA+VELC QAG    +      TQ          + GG           S +  PL++
Sbjct: 247 RIPALVELCIQAGVEIPEYPCRRRTQPIRMMGKRVIDRGGYHKEPEKPQTSSSLSSPLAE 306

Query: 214 ---------------HEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSG 273
                           ++  +A ET+ A+E +R GV KL+  +  K C YCSEVHVGP G
Sbjct: 307 LDTLGVFERYPPPTPEDIPKIAQETMDAYEKVRLGVTKLMRKFTVKACGYCSEVHVGPWG 366

Query: 274 HKARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVV 325
           H  +LCG F ++   G H W+ A VD++ PP  VWH R      L    + +YG APA+V
Sbjct: 367 HSVKLCGEFKHQWRDGKHGWQDALVDEVFPPNYVWHVRDLKGNPLTGNLRRFYGKAPALV 426

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004151879.14.6e-193100.00APO protein 4, mitochondrial [Cucumis sativus] >XP_031737269.1 APO protein 4, mi... [more]
KAA0043413.14.2e-18696.65APO protein 4 [Cucumis melo var. makuwa][more]
XP_008455841.17.8e-18596.04PREDICTED: APO protein 4, mitochondrial [Cucumis melo] >XP_008455842.1 PREDICTED... [more]
XP_038901944.12.6e-17287.27APO protein 4, mitochondrial [Benincasa hispida] >XP_038901945.1 APO protein 4, ... [more]
XP_023532078.13.3e-15982.87APO protein 4, mitochondrial [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9LSZ02.1e-10057.53APO protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO4 PE=2 SV=2[more]
Q8W4A51.0e-5736.61APO protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO2 PE=2 SV=1[more]
Q9XIR49.7e-5335.08APO protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO1 PE=2 SV=1[more]
Q9FH501.3e-4433.13APO protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LQG52.2e-193100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G421010 PE=4 SV=1[more]
A0A5A7TJ182.0e-18696.65APO protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1639G00180... [more]
A0A1S3C3433.8e-18596.04APO protein 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495942 PE=4 SV=1[more]
A0A5D3BGQ13.8e-18596.04APO protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold232G00100 ... [more]
A0A6J1I7G71.6e-15983.44APO protein 4, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111469835 PE... [more]
Match NameE-valueIdentityDescription
AT3G21740.11.5e-10157.53Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT5G57930.17.1e-5936.61Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT5G57930.27.1e-5936.61Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT1G64810.16.9e-5435.08Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT1G64810.26.9e-5435.08Arabidopsis thaliana protein of unknown function (DUF794) [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023342APO domainPFAMPF05634APO_RNA-bindcoord: 30..167
e-value: 8.9E-40
score: 136.2
coord: 196..309
e-value: 7.3E-20
score: 71.2
IPR023342APO domainPROSITEPS51499APOcoord: 85..170
score: 22.228788
IPR023342APO domainPROSITEPS51499APOcoord: 226..311
score: 18.924055
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 168..184
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 168..189
NoneNo IPR availablePANTHERPTHR10388:SF20APO PROTEIN 4, MITOCHONDRIALcoord: 12..326
NoneNo IPR availablePANTHERPTHR10388EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1coord: 12..326

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_2G035140.1CsaV3_2G035140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003723 RNA binding