CsaV3_2G034070 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_2G034070
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 4
Locationchr2: 22567066 .. 22570631 (+)
RNA-Seq ExpressionCsaV3_2G034070
SyntenyCsaV3_2G034070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAAATGGAAAAAAGAGTATGAATGGAAAACTTTACCCACAAACTCTACAATTAAACTTCAAAAGGAAAAAACGTTTGGAGTTGGATCACCATTGGATTCCCAAATGGAATAAAATCAAAATAGTAATAACTCTTGAAGGAAGAAGATGATTTTCCTTTAATTAATTTTGCTTAGTTGTCGTCTTCTAATTCGAATCGCCATAGCCACCACAGGCCACACCCATAGCCATATCCCAACTCCTACTTTCTTGTGTATCGTGCATAACAGAACGCAGGGCAGTGATGAATCGCCAACCGCTATGATGGAGATTTTTCTAATTAATTATCATAAACAACCATTTTTATTTTGCCACCCCATTTCCTATCAATCGACCCCACGTTATCATTCCCATTTCTCACCTCCTGCGCTGTGTTTTGTCCCACCCATTTCTCCCAATTCCCTTTTAGAGGCAACCCCATTTCGTTTTGATTATCTAATTTTGCTTATTTTCTTTAAGCACGCAGAACCCAGGGAGATTTCTGTTTCTGGGCATTGATGGGTCGGTGGAATGACAAACTTGTTGCCTTTATCAACAATAGATGGTTGGTTTTTGTTGCTGCAATATGGCTTCAATCTTGGGCTGGAATTGGATATCTTTTTGGAAGTATATCGCCGATAATCAAAACCAATTTGAGTTACAATCAGAGACAAATTTCTCGTCTTGGTGTCGCTAAGGATCTTGGCGATAGCGTTGGCTTTTTGGCTGCAACGTTAACGGAGATATTGCCCTTTTGGGGAAGTCTCTTGGTCGGTGCTATCCATAACTTTGTTGGGTATGGTTGGGTTTGGCTCATCGTCACTGGTCGAGCTCCCGTTTTGCCTCTCTGGGCTGTAAGTTTTTTGCTCTTTATCTTTTAATGCTCTCTATTTGAGTTTTGATCTTTGTCTTTAGATGTTTCTAATAACATATCCATATTCAAATCTTGATTGAATGGCTTCTGATTAAGATGTTTTGCTTTTGCGAACTAATTTCAAAAGTTGCATAGTGATTAGCGGTCAACGAGTTGGAATCTGTTGTTGGCTTCAAAAGTGAATCTGGGGATGGGGAGATGATTCTTTCGTGGTTAGCCTTAATGAGGATAGTTAGATTAAAGATAGTTTGCTACTTGTCTTAGAATTTTCTCAGAATGGTTAGTCTAAGTTGTGTTGTAGCAAAATGCAGCGGGATTCAGATTAACTGTTTGATAATTTGTTTCTACGACTATAGAAGCATTTTTATGGAATATTTCATGGATGCAGAACTGTTTGTTGTGGATAGTACTTTACAAACAGATCTGCTGAATATATAGTTTGGTTGAGGCTATTGAAAAGTCCTTTTGGAAGTTCCAGAATCAATCCTTCCCAACCTGTTCATTAAGATTGGTAAAATGCATCATCTTCTAGCCTTTTTACTCTATAGTCAAAGGAAATGAGGGGTGAGATGCACGAATGGCTGTAACCAAACCACTCTAACCAATTAAAGTTTGAAGAACAAACTGTTGAGCTATTTTCTTCTTTTACCATTTTTGTTTAGTTAAGTATGGATACCATTTTGCAGATGTGCGCTCTTGTATTTATTGGAACGAATGGGGAGACATACTTCAATACAGTTTCTTTGGTTTCTTGTGTACAAAACTTCCCGAAAAGCAGAGGCCCTGTGGTCGGGATTCTGAAAGGCTTTGCTGGTTTAAGTGGTGCTATTTTGACTCAGACATATGCAATCTTCCATTCTCCTGAATCAGCCAATCTTATATTTATGGTTGCAGTAGGCCCTGCACTGGTGGCTATTGGTGTGATGTTTTTTATCAGACCTGTTGCCGGTCATCGACAAGTAAGGCCGTCCGATGGCATGAGCTTTACTAGTGTTTATGGTGTCTGCCTCCTTTTGGCTGCATATTTGATGGGAGTCATGCTTATTGAGGATCTTGTTACCTTGAGCCCCATTGTGATAACCATATTTACTGTGGTTATGTTTGTCATCCTTCTTACGCCCTTTTTTATTCCCGTGTCATTGACTCTTAGCTCGGAGGCAACAACATATGCTGAGCAAGAGGCTCTCTTGCCACCGTCTGAGAAAGAGGAACCTGCTAGAACTGAGCCAGATGGTAATGAAGTGATATTTAGTGAGGTGGAAGATGAAAAATCCGAGGGAGAGGACTTACTTCCTGCATCAGAGAGACAAAAACGCATTGCTCAGTTGCAAGCAAAACTATTGCAAGCAGCCGCAGAAGGAGCAGTAAGAGTCAAGAGGAGGAAGGGTCCACGTCGAGGGGAAGACTTCACTTTGGGTCAAGCTTTAATCAAAGCAGACTTTTGGCTTATATTTTCCTCCCATCTTCTTGGTTCTGGAACTGGATTGACCGTGATCGATAACCTCGGACAGATGAGCCAGTCTTTAGGTTACGATAATACACATATCTTTGTATCCCTGATCAGCATATGGAACTTTCTCGGCCGTGTGGGTGGTGGATATCTATCTGAGATTGTTGTGAGGTACAAAAGTTTGTTTTAGTTGTCTGAACGCCAGTAAATAAAACTTTTGTTCAATTATATCTTACTGTCATTTTCTTCAACAGAGATTTTGCTTATCCAAGACCCATAGCAATGACGATTGCGCAAGTCCTTATGATATTTGGACATGTCTTCATCGGTATGGGATGGCCCGGGGCAATGTACATTGGCACTCTCATAACGGGGCTCGGGTACGGGGCTCACTGGGCCATTGTACCTGCTACTGCCTCCGAATTGTTTGGGTTGAAAAAGTTTGGCGCTTTGTACAACTTCATCACTCTCTCAACTCCTATGGGATCTTTGGTATTTTCTGGCTTAATCGCGAGCAGTATATATGATAGTGAAGCCGAGAAGCAAGCTCGTAATCATCTCACACAGTTCCAAAGCTCCTCGTCATTTTGGTTCACCAGACTTTACACAGAGGGACCGCACAAGTGCGAGGGCGCCATATGCTTCTTTTTAACTTGTATGATAATGGGTGGATTCTGCGCGATTGCAGCGATATTAAGTTTAATTTTGGTGCACCGGACAAAGGGTGTTTACCACAACCTCTATGGAAAATCTCGTACATCAACACTTTCATAGAGTGGTTTCAAGTCTCATATCAATTGTGCTACCGTTTTGCGTGTTCGTTATTTAGAGATCGAGTTGAATTTTTTGCTCGGATGATGGACTTTGAAAGAAGCATTTTCCAAGAGCCAAGGACTCGAAATCGACTTGCTTTCACTGTTACAATTCACCCTTTTTTGGTATGAAGAAGCTTTTTTGCCTGTGTGTTGGAGAATGAAGTTGGGTGGAAGAAGATCATACATACACCTTCATGTCCAATCTTCCACCGGAAAGAAAAAAATTGTATGAAATATAATTGTTGTGAAATATTTGATATTTAAGATTCACCATTATTGATCATTGAATAAAAAGAAAATGTATGCTATTTATTTATTTTATTTTATATTTTTTTGTTGTATTCTCTCTTTAGATAGTCATGGATAGGCTTTGTATAATTGTGATTTTTTCAATATTTATTGGAGAACCA

mRNA sequence

ATGGGTCGGTGGAATGACAAACTTGTTGCCTTTATCAACAATAGATGGTTGGTTTTTGTTGCTGCAATATGGCTTCAATCTTGGGCTGGAATTGGATATCTTTTTGGAAGTATATCGCCGATAATCAAAACCAATTTGAGTTACAATCAGAGACAAATTTCTCGTCTTGGTGTCGCTAAGGATCTTGGCGATAGCGTTGGCTTTTTGGCTGCAACGTTAACGGAGATATTGCCCTTTTGGGGAAGTCTCTTGGTCGGTGCTATCCATAACTTTGTTGGGTATGGTTGGGTTTGGCTCATCGTCACTGGTCGAGCTCCCGTTTTGCCTCTCTGGGCTATGTGCGCTCTTGTATTTATTGGAACGAATGGGGAGACATACTTCAATACAGTTTCTTTGGTTTCTTGTGTACAAAACTTCCCGAAAAGCAGAGGCCCTGTGGTCGGGATTCTGAAAGGCTTTGCTGGTTTAAGTGGTGCTATTTTGACTCAGACATATGCAATCTTCCATTCTCCTGAATCAGCCAATCTTATATTTATGGTTGCAGTAGGCCCTGCACTGGTGGCTATTGGTGTGATGTTTTTTATCAGACCTGTTGCCGGTCATCGACAAGTAAGGCCGTCCGATGGCATGAGCTTTACTAGTGTTTATGGTGTCTGCCTCCTTTTGGCTGCATATTTGATGGGAGTCATGCTTATTGAGGATCTTGTTACCTTGAGCCCCATTGTGATAACCATATTTACTGTGGTTATGTTTGTCATCCTTCTTACGCCCTTTTTTATTCCCGTGTCATTGACTCTTAGCTCGGAGGCAACAACATATGCTGAGCAAGAGGCTCTCTTGCCACCGTCTGAGAAAGAGGAACCTGCTAGAACTGAGCCAGATGGTAATGAAGTGATATTTAGTGAGGTGGAAGATGAAAAATCCGAGGGAGAGGACTTACTTCCTGCATCAGAGAGACAAAAACGCATTGCTCAGTTGCAAGCAAAACTATTGCAAGCAGCCGCAGAAGGAGCAGTAAGAGTCAAGAGGAGGAAGGGTCCACGTCGAGGGGAAGACTTCACTTTGGGTCAAGCTTTAATCAAAGCAGACTTTTGGCTTATATTTTCCTCCCATCTTCTTGGTTCTGGAACTGGATTGACCGTGATCGATAACCTCGGACAGATGAGCCAGTCTTTAGGTTACGATAATACACATATCTTTGTATCCCTGATCAGCATATGGAACTTTCTCGGCCGTGTGGGTGGTGGATATCTATCTGAGATTGTTGTGAGAGATTTTGCTTATCCAAGACCCATAGCAATGACGATTGCGCAAGTCCTTATGATATTTGGACATGTCTTCATCGGTATGGGATGGCCCGGGGCAATGTACATTGGCACTCTCATAACGGGGCTCGGGTACGGGGCTCACTGGGCCATTGTACCTGCTACTGCCTCCGAATTGTTTGGGTTGAAAAAGTTTGGCGCTTTGTACAACTTCATCACTCTCTCAACTCCTATGGGATCTTTGGTATTTTCTGGCTTAATCGCGAGCAGTATATATGATAGTGAAGCCGAGAAGCAAGCTCGTAATCATCTCACACAGTTCCAAAGCTCCTCGTCATTTTGGTTCACCAGACTTTACACAGAGGGACCGCACAAGTGCGAGGGCGCCATATGCTTCTTTTTAACTTGTATGATAATGGGTGGATTCTGCGCGATTGCAGCGATATTAAGTTTAATTTTGGTGCACCGGACAAAGGGTGTTTACCACAACCTCTATGGAAAATCTCGTACATCAACACTTTCATAG

Coding sequence (CDS)

ATGGGTCGGTGGAATGACAAACTTGTTGCCTTTATCAACAATAGATGGTTGGTTTTTGTTGCTGCAATATGGCTTCAATCTTGGGCTGGAATTGGATATCTTTTTGGAAGTATATCGCCGATAATCAAAACCAATTTGAGTTACAATCAGAGACAAATTTCTCGTCTTGGTGTCGCTAAGGATCTTGGCGATAGCGTTGGCTTTTTGGCTGCAACGTTAACGGAGATATTGCCCTTTTGGGGAAGTCTCTTGGTCGGTGCTATCCATAACTTTGTTGGGTATGGTTGGGTTTGGCTCATCGTCACTGGTCGAGCTCCCGTTTTGCCTCTCTGGGCTATGTGCGCTCTTGTATTTATTGGAACGAATGGGGAGACATACTTCAATACAGTTTCTTTGGTTTCTTGTGTACAAAACTTCCCGAAAAGCAGAGGCCCTGTGGTCGGGATTCTGAAAGGCTTTGCTGGTTTAAGTGGTGCTATTTTGACTCAGACATATGCAATCTTCCATTCTCCTGAATCAGCCAATCTTATATTTATGGTTGCAGTAGGCCCTGCACTGGTGGCTATTGGTGTGATGTTTTTTATCAGACCTGTTGCCGGTCATCGACAAGTAAGGCCGTCCGATGGCATGAGCTTTACTAGTGTTTATGGTGTCTGCCTCCTTTTGGCTGCATATTTGATGGGAGTCATGCTTATTGAGGATCTTGTTACCTTGAGCCCCATTGTGATAACCATATTTACTGTGGTTATGTTTGTCATCCTTCTTACGCCCTTTTTTATTCCCGTGTCATTGACTCTTAGCTCGGAGGCAACAACATATGCTGAGCAAGAGGCTCTCTTGCCACCGTCTGAGAAAGAGGAACCTGCTAGAACTGAGCCAGATGGTAATGAAGTGATATTTAGTGAGGTGGAAGATGAAAAATCCGAGGGAGAGGACTTACTTCCTGCATCAGAGAGACAAAAACGCATTGCTCAGTTGCAAGCAAAACTATTGCAAGCAGCCGCAGAAGGAGCAGTAAGAGTCAAGAGGAGGAAGGGTCCACGTCGAGGGGAAGACTTCACTTTGGGTCAAGCTTTAATCAAAGCAGACTTTTGGCTTATATTTTCCTCCCATCTTCTTGGTTCTGGAACTGGATTGACCGTGATCGATAACCTCGGACAGATGAGCCAGTCTTTAGGTTACGATAATACACATATCTTTGTATCCCTGATCAGCATATGGAACTTTCTCGGCCGTGTGGGTGGTGGATATCTATCTGAGATTGTTGTGAGAGATTTTGCTTATCCAAGACCCATAGCAATGACGATTGCGCAAGTCCTTATGATATTTGGACATGTCTTCATCGGTATGGGATGGCCCGGGGCAATGTACATTGGCACTCTCATAACGGGGCTCGGGTACGGGGCTCACTGGGCCATTGTACCTGCTACTGCCTCCGAATTGTTTGGGTTGAAAAAGTTTGGCGCTTTGTACAACTTCATCACTCTCTCAACTCCTATGGGATCTTTGGTATTTTCTGGCTTAATCGCGAGCAGTATATATGATAGTGAAGCCGAGAAGCAAGCTCGTAATCATCTCACACAGTTCCAAAGCTCCTCGTCATTTTGGTTCACCAGACTTTACACAGAGGGACCGCACAAGTGCGAGGGCGCCATATGCTTCTTTTTAACTTGTATGATAATGGGTGGATTCTGCGCGATTGCAGCGATATTAAGTTTAATTTTGGTGCACCGGACAAAGGGTGTTTACCACAACCTCTATGGAAAATCTCGTACATCAACACTTTCATAG

Protein sequence

MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAKDLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIGTNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMVAVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSPIVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEVIFSEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALIKADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSEIVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASELFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRLYTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRTSTLS*
Homology
BLAST of CsaV3_2G034070 vs. NCBI nr
Match: XP_011649956.1 (protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Cucumis sativus] >KGN63164.1 hypothetical protein Csa_021870 [Cucumis sativus])

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAK 60
           MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAK
Sbjct: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIG 120
           DLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIG
Sbjct: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMV 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMV
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMV 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSP
Sbjct: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSP 240

Query: 241 IVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
           IVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEVIF
Sbjct: 241 IVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE
Sbjct: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRL 540
           LFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRL
Sbjct: 481 LFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRL 540

Query: 541 YTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRTSTLS 597
           YTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRTSTLS
Sbjct: 541 YTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRTSTLS 596

BLAST of CsaV3_2G034070 vs. NCBI nr
Match: XP_008441326.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Cucumis melo] >KAA0056548.1 protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Cucumis melo var. makuwa] >TYK02714.1 protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 576/596 (96.64%), Postives = 584/596 (97.99%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAK 60
           MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISP+IKTNLSYNQRQ+SRLGVAK
Sbjct: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIG 120
           DLGDSVGFLAATLTEILPFWGSLLVGAIHN VGYGWVWLIVTGRAPVLPLWAMC LVF+G
Sbjct: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMV 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQ YAI HSP+SANLIFMV
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSF+SVYGVCLLLAAYLMGVMLIEDLVTLSP
Sbjct: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240

Query: 241 IVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
            VITIFTVVMFVILLTPF IPV+LT SSE TTYAEQEALLPPSEKEEPARTEPDGNEVIF
Sbjct: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE
Sbjct: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRL 540
           LFGLKKFGALYNFITLSTPMGSL+FSGLIASSIYDSEAEKQARNHLTQFQSSSS WFTRL
Sbjct: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540

Query: 541 YTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRTSTLS 597
           Y EGPHKCEGAICFFLTCMIM GFCAIA ILSLILV+RTKGVYHNLYGKSRTSTLS
Sbjct: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 596

BLAST of CsaV3_2G034070 vs. NCBI nr
Match: XP_038885267.1 (protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Benincasa hispida])

HSP 1 Score: 1053.5 bits (2723), Expect = 6.8e-304
Identity = 537/596 (90.10%), Postives = 562/596 (94.30%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAK 60
           MGRWN+KLVAF NNRWLVFVAAIWLQS AGIGYLFGSISP+IKTNLSYNQRQI+RLGVAK
Sbjct: 1   MGRWNEKLVAFFNNRWLVFVAAIWLQSCAGIGYLFGSISPVIKTNLSYNQRQIARLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIG 120
           DLGDSVG LAATL+EILPFWGSLLVGAIHNFVGYGW+WLIVTGRAPVLPLWAMC LVF+G
Sbjct: 61  DLGDSVGILAATLSEILPFWGSLLVGAIHNFVGYGWIWLIVTGRAPVLPLWAMCILVFLG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMV 180
           TNGETYFNTV+LVSCVQNFPKSRGPVVGILKGFAGLSGAILTQ YAI H+P+SANLIFMV
Sbjct: 121 TNGETYFNTVALVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIVHAPDSANLIFMV 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIGVMFFIRPVAGHRQVRPSD MSFTSVYGVCLLLAAYLMGVML+EDLVTL+P
Sbjct: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDSMSFTSVYGVCLLLAAYLMGVMLVEDLVTLNP 240

Query: 241 IVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
            VI IFT VMFVILLTPF IPV+LT SSE  TYAEQEALLPPSEK+EPAR+EPDGNEVIF
Sbjct: 241 TVIAIFTGVMFVILLTPFLIPVTLTFSSETMTYAEQEALLPPSEKQEPARSEPDGNEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPA ERQKRIAQLQA+LLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIF S LLGSGTGLTVIDNLGQMS+SLGYDNTHIFVSLISIWNFLGRVGGGYLSE
Sbjct: 361 KADFWLIFVSLLLGSGTGLTVIDNLGQMSESLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDFAYPRPIAM IAQ LMIFGH+FIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDFAYPRPIAMAIAQFLMIFGHLFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRL 540
           LFGLKKFGALYNF+TLS PMGSL+FS LIAS IYDSEAEKQA NH    QSSSSF  +RL
Sbjct: 481 LFGLKKFGALYNFLTLSNPMGSLIFSSLIASGIYDSEAEKQAHNHPPHLQSSSSFLLSRL 540

Query: 541 YTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRTSTLS 597
           Y +GPH+CEGAICFFLTCM+M G CAIA +LSLILV+RTKGVY NLYGKSRTSTLS
Sbjct: 541 YADGPHECEGAICFFLTCMLMAGLCAIAGMLSLILVYRTKGVYSNLYGKSRTSTLS 596

BLAST of CsaV3_2G034070 vs. NCBI nr
Match: XP_023544039.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1035.4 bits (2676), Expect = 1.9e-298
Identity = 527/596 (88.42%), Postives = 558/596 (93.62%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAK 60
           MGR N+KLVAF+NNRWLVFVAAIW+QS AGIGYLFGSISP+IKTNLSYNQ+QI+RLGVAK
Sbjct: 1   MGRVNEKLVAFLNNRWLVFVAAIWIQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIG 120
           DLGDSVG LA TL+EILPFWG+LLVGA++NF+GYGWVWLIVTGRAPVLPLWAMC LVF+G
Sbjct: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMV 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQ YAI H P+SANLIFM+
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIG+MFFIRPVAGHRQVRPSDGMSF+SVYGVCLLLAAYLMGVML+EDLV LSP
Sbjct: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240

Query: 241 IVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
            VI IFT VMFVILLTPF IPV LT SSE T Y EQEALL  S K EPAR+EPDG+EVIF
Sbjct: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETTAYPEQEALLQQSPKHEPARSEPDGHEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPASERQKRIAQLQA+LLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIF S LLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGY SE
Sbjct: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRD+AYPRPIAM  AQ LMIFGH+FIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRL 540
           LFGLKKFGALYNF+TLSTPMGSL+FSGLIASSIYDSEAEKQA N L+Q QSSSS WF+RL
Sbjct: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540

Query: 541 YTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRTSTLS 597
           + + P KC+GAICFFLTCMIM GFCAIA +LSLILVHRTKGVY+NLYGKSR STLS
Sbjct: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 596

BLAST of CsaV3_2G034070 vs. NCBI nr
Match: KAG6602685.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1034.2 bits (2673), Expect = 4.2e-298
Identity = 526/596 (88.26%), Postives = 559/596 (93.79%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAK 60
           MGR N+KLVAF+NNRWLVFVAAIW+QS AGIGYLFGSISP+IKTNLSYNQ+QI+RLGVAK
Sbjct: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIG 120
           DLGDSVG LA TL+EILPFWG+LLVGA++NF+GYGWVWLIVTGRAPVLPLWAMC LVF+G
Sbjct: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMV 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQ YAI H P+SANLIFM+
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIG+MFFIRPVAGHRQVRPSDG+SF+SVYGVCLLLAAYLMGVML+EDLV LSP
Sbjct: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGVSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240

Query: 241 IVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
            VI IFT VMFVILLTPF IPV LT SSE T Y EQEALL  S K+EPAR+EPDG+EVIF
Sbjct: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETTAYPEQEALLQQSPKQEPARSEPDGHEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPASERQKRIAQLQA+LLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIF S LLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGY SE
Sbjct: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRD+AYPRPIAM  AQ LMIFGH+FIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRL 540
           LFGLKKFGALYNF+TLSTPMGSL+FSGLIASSIYDSEAEKQA N L+Q QSSSS WF+RL
Sbjct: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540

Query: 541 YTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRTSTLS 597
           + + P KC+GAICFFLTCMIM GFCAIA +LSLILVHRTKGVY+NLYGKSR STLS
Sbjct: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 596

BLAST of CsaV3_2G034070 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 6.4e-31
Identity = 150/591 (25.38%), Postives = 256/591 (43.32%), Query Frame = 0

Query: 2   GRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAKD 61
           GRW          +W V VAAIW+Q+  G  + F + S  +K+ L  +Q +++ L VA D
Sbjct: 39  GRW---------RKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASD 98

Query: 62  LGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTG--RAPVLPLWAMCALVFI 121
           LG + G+ +       P    L   A   FVGYG  WL++T     P   ++  C L  +
Sbjct: 99  LGKAFGWSSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSLVFLCCLLAGL 158

Query: 122 GTNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFM 181
                 +FNT   + C+++FP +R   + +   F G+S A+ +  +    +P S+NL  +
Sbjct: 159 SI---CWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAI-NPSSSNLYLL 218

Query: 182 V-AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTS-VYGVCLLLAAYLMGVMLIEDLVT 241
           + ++ P +V+   ++ +            D     S V+ +  +LA      +L+    T
Sbjct: 219 LNSLVPLVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSST 278

Query: 242 LSPIVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNE 301
            S  +  I  VV+ V             L +    YA ++  LP                
Sbjct: 279 SSARLNFIGAVVLLV-----------FPLCAPLLVYA-RDYFLP---------------- 338

Query: 302 VIFSEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQ 361
           VI + +  E S G  +L   E + +   + +K      E     K     R G++ +   
Sbjct: 339 VINARLNHE-SSGYVMLNIDELKNQKTSVSSK---TGYEHMGTAKEGNTVRLGDEHSFRL 398

Query: 362 ALIKADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGY 421
            + + +FWL + ++  G   GL   +NLGQ++QSLG ++T + V++ S ++F GR+    
Sbjct: 399 LISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNSTTL-VTIYSSFSFFGRLLSA- 458

Query: 422 LSEIVVRDFAYPRP--IAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVP 481
             + + + F   R    A+ +    + F  + +      A+   T + GL  G  +A   
Sbjct: 459 APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAV 518

Query: 482 ATASELFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSF 541
           +  S+LFG    G  +N +  + P+GSL++ G IA+SIY++ A       +T   S S  
Sbjct: 519 SITSDLFGPNSVGVNHNILITNIPIGSLLY-GYIAASIYEANASPD----ITPIVSDSIV 565

Query: 542 WFTRLYTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNL 587
                       C G  C+F T +  G    +  + SL L  RTK VYH L
Sbjct: 579 ------------CIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHRL 565

BLAST of CsaV3_2G034070 vs. ExPASy TrEMBL
Match: A0A0A0LQ60 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G406130 PE=4 SV=1)

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAK 60
           MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAK
Sbjct: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIG 120
           DLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIG
Sbjct: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMV 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMV
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMV 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSP
Sbjct: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSP 240

Query: 241 IVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
           IVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEVIF
Sbjct: 241 IVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE
Sbjct: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRL 540
           LFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRL
Sbjct: 481 LFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRL 540

Query: 541 YTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRTSTLS 597
           YTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRTSTLS
Sbjct: 541 YTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRTSTLS 596

BLAST of CsaV3_2G034070 vs. ExPASy TrEMBL
Match: A0A5A7UN83 (Protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1154G00120 PE=4 SV=1)

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 576/596 (96.64%), Postives = 584/596 (97.99%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAK 60
           MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISP+IKTNLSYNQRQ+SRLGVAK
Sbjct: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIG 120
           DLGDSVGFLAATLTEILPFWGSLLVGAIHN VGYGWVWLIVTGRAPVLPLWAMC LVF+G
Sbjct: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMV 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQ YAI HSP+SANLIFMV
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSF+SVYGVCLLLAAYLMGVMLIEDLVTLSP
Sbjct: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240

Query: 241 IVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
            VITIFTVVMFVILLTPF IPV+LT SSE TTYAEQEALLPPSEKEEPARTEPDGNEVIF
Sbjct: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE
Sbjct: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRL 540
           LFGLKKFGALYNFITLSTPMGSL+FSGLIASSIYDSEAEKQARNHLTQFQSSSS WFTRL
Sbjct: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540

Query: 541 YTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRTSTLS 597
           Y EGPHKCEGAICFFLTCMIM GFCAIA ILSLILV+RTKGVYHNLYGKSRTSTLS
Sbjct: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 596

BLAST of CsaV3_2G034070 vs. ExPASy TrEMBL
Match: A0A1S3B357 (protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485475 PE=4 SV=1)

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 576/596 (96.64%), Postives = 584/596 (97.99%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAK 60
           MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISP+IKTNLSYNQRQ+SRLGVAK
Sbjct: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIG 120
           DLGDSVGFLAATLTEILPFWGSLLVGAIHN VGYGWVWLIVTGRAPVLPLWAMC LVF+G
Sbjct: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMV 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQ YAI HSP+SANLIFMV
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSF+SVYGVCLLLAAYLMGVMLIEDLVTLSP
Sbjct: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240

Query: 241 IVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
            VITIFTVVMFVILLTPF IPV+LT SSE TTYAEQEALLPPSEKEEPARTEPDGNEVIF
Sbjct: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE
Sbjct: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRL 540
           LFGLKKFGALYNFITLSTPMGSL+FSGLIASSIYDSEAEKQARNHLTQFQSSSS WFTRL
Sbjct: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540

Query: 541 YTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRTSTLS 597
           Y EGPHKCEGAICFFLTCMIM GFCAIA ILSLILV+RTKGVYHNLYGKSRTSTLS
Sbjct: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 596

BLAST of CsaV3_2G034070 vs. ExPASy TrEMBL
Match: A0A6J1E533 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111430673 PE=4 SV=1)

HSP 1 Score: 1032.3 bits (2668), Expect = 7.8e-298
Identity = 525/596 (88.09%), Postives = 559/596 (93.79%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAK 60
           MGR N+KLVAF+NNRWLVFVAAIW+QS AGIGYLFGSISP+IKTNLSYNQ+QI+RLGVAK
Sbjct: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIG 120
           DLGDSVG LA TL+EILPFWG+LLVGA++NF+GYGWVWLIVTGRAPVLPLWAMC LVF+G
Sbjct: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMV 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQ YAI H P+SANLIFM+
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIG+MFFIRPVAGHRQVRPSDG+SF+SVYGVCLLLAAYLMGVML+EDLV LSP
Sbjct: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGVSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240

Query: 241 IVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
            VI IFT VMFVILLTPF IPV LT SSE T Y EQEALL  S K+EPAR+EPDG+EVIF
Sbjct: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETTAYPEQEALLQQSPKQEPARSEPDGHEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPASERQKRIAQLQA+LLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIF S LLGSGTGLTVIDNLGQMSQSLGY+NTHIFVSLISIWNFLGRVGGGY SE
Sbjct: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYNNTHIFVSLISIWNFLGRVGGGYFSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRD+AYPRPIAM  AQ LMIFGH+FIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRL 540
           LFGLKKFGALYNF+TLSTPMGSL+FSGLIASSIYDSEAEKQA N L+Q QSSSS WF+RL
Sbjct: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540

Query: 541 YTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRTSTLS 597
           + + P KC+GAICFFLTCMIM GFCAIA +LSLILVHRTKGVY+NLYGKSR STLS
Sbjct: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 596

BLAST of CsaV3_2G034070 vs. ExPASy TrEMBL
Match: A0A6J1JLV2 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111487059 PE=4 SV=1)

HSP 1 Score: 1031.2 bits (2665), Expect = 1.7e-297
Identity = 525/596 (88.09%), Postives = 557/596 (93.46%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAK 60
           MGR N+KLVAF+NNRWLVFVAAIW+QS AGIGYLFGSISP+IKTNLSYNQ+QI+RLGVAK
Sbjct: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIG 120
           DLGDSVG LA TL+EILPFWG+LLVGA++NF+GYGWVWLIVTGRAPVLPLWAMC LVF+G
Sbjct: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMV 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQ YAI H P+SANLIFM+
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIG+MFFIRPVAGHRQVRPSDGMSF+SVYGVCLLLAAYLMGVML+EDLV LSP
Sbjct: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240

Query: 241 IVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
            VI IFT VMFVILLTPF IPV LT SSE   Y EQEALL  S K+EPAR+EPDG+EVIF
Sbjct: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPA ERQKRIAQLQA+LLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIF S LLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGY SE
Sbjct: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRD+AYPRPIAM  AQ LMIFGH+FIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRL 540
           LFGLKKFGALYNF+TLSTPMGSL+FSGLIASSIYDSEAEKQA N L+Q QSSSS WF+RL
Sbjct: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540

Query: 541 YTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRTSTLS 597
           + + P KC+GAICFFLTCMIM GFCAIA +LSLILVHRTKGVY+NLYGKSR STLS
Sbjct: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 596

BLAST of CsaV3_2G034070 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 803.5 bits (2074), Expect = 1.1e-232
Identity = 408/589 (69.27%), Postives = 484/589 (82.17%), Query Frame = 0

Query: 6   DKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAKDLGDS 65
           +K V+FINNRWLVFVAA+W+QS AGIGYLFGSISP+IK++L+YNQ+++SRLGVAKDLGDS
Sbjct: 7   EKFVSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDS 66

Query: 66  VGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIGTNGET 125
           VGF+A TL+EILP W +LLVGA+ N +GYGWVWLIVTGRAP+LPLWAMC L+F+G NGET
Sbjct: 67  VGFIAGTLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGET 126

Query: 126 YFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMVAVGPA 185
           YFNT +LVS VQNFPKSRGPVVGILKGFAGL GAI++Q Y + HS   A+LI MVAV PA
Sbjct: 127 YFNTGALVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPA 186

Query: 186 LVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSPIVITI 245
           +V + +MFFIRPV GH+Q+RP+DG SFT +YGVCLLLAAYLM VMLI+DLV +S  VIT+
Sbjct: 187 VVVVCLMFFIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITV 246

Query: 246 FTVVMFVILLTPFFIPVSLTLSSEATTYAE--QEALLPPSEKEEPARTEPDGNEVIFSEV 305
           FT+V+FVIL+ P  +P+  +  +E     +  +E L+P  E +EP    PD   +I SEV
Sbjct: 247 FTIVLFVILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPD---LILSEV 306

Query: 306 EDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALIKAD 365
           EDEK +  DLLPASER KRIA LQA+L+QAAAEGAVRV RR+GP RGEDFTL QAL+KAD
Sbjct: 307 EDEKPKDVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKAD 366

Query: 366 FWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSEIVV 425
           FWLIF S LLGSG+GLTVIDNLGQMSQSLGYDNTH+ VS+ISIWNFLGR+GGGY SE+VV
Sbjct: 367 FWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVLVSMISIWNFLGRIGGGYFSELVV 426

Query: 426 RDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASELFG 485
           RD+AYPRP+AM +AQ++M  GH+F   GWPGAMYIGTL+ GLGYGAHWAIVPATASELFG
Sbjct: 427 RDYAYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFG 486

Query: 486 LKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRLYTE 545
           LKKFGALYNF+TL+ P GSLVFSG+IASSIYD EAE+QA  H + F    +         
Sbjct: 487 LKKFGALYNFLTLANPAGSLVFSGMIASSIYDREAERQA--HGSVFDPDDAL-------- 546

Query: 546 GPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRT 593
              +C G+ICFFLT +IM GFC IA +LS+ILV RTK VY +LYGK+RT
Sbjct: 547 ---RCNGSICFFLTSLIMSGFCIIACMLSMILVRRTKSVYTHLYGKTRT 579

BLAST of CsaV3_2G034070 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 802.0 bits (2070), Expect = 3.3e-232
Identity = 409/590 (69.32%), Postives = 486/590 (82.37%), Query Frame = 0

Query: 6   DKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAKDLGDS 65
           +++ +FINNRWLVFVAA+W+QS AGIGYLFGSISP+IK++L+YNQ+Q+SRLGVAKDLGDS
Sbjct: 7   ERVKSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDS 66

Query: 66  VGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIGTNGET 125
           VGFLA TL+EILP W +LLVG++ N VGYGWVWLIVTGRAP+LPLWAMC L+F+G NGET
Sbjct: 67  VGFLAGTLSEILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGET 126

Query: 126 YFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMVAVGPA 185
           YFNT +LVS VQNFPKSRGPVVGILKGFAGL GAIL+Q Y + HS + A+LIFMVAV P+
Sbjct: 127 YFNTAALVSGVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSDRASLIFMVAVAPS 186

Query: 186 LVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSPIVITI 245
           +V + +MFFIRPV GHRQ+R SD  SFT +Y VC+LLAAYLM VML+ED + LS  +I  
Sbjct: 187 VVVVPLMFFIRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIIIA 246

Query: 246 FTVVMFVILLTPFFIPVSLTLSSEATTYAE--QEALLPPSEKEEPAR-TEPD-GNEVIFS 305
           FTVV+F ILL P FIP++ +  + +T   +  +E LL   + ++P + T PD G E+IFS
Sbjct: 247 FTVVLFAILLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELIFS 306

Query: 306 EVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALIK 365
           EVEDEK +  DLLPA ER KRIAQLQAKL+QAAAEGAVRVKRR+GP RGEDFTL QAL+K
Sbjct: 307 EVEDEKPKEVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVK 366

Query: 366 ADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSEI 425
           ADFWLIF S LLGSG+GLTVIDNLGQMSQSLGYDNTH+FVS+ISIWNFLGR+GGGY SE+
Sbjct: 367 ADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVFVSMISIWNFLGRIGGGYFSEL 426

Query: 426 VVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASEL 485
           +VRD+AYPRP+A+ +AQ++M  GH+F   GWPGAM+IGTL+ GLGYGAHWAIVPATASEL
Sbjct: 427 IVRDYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPATASEL 486

Query: 486 FGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRLY 545
           FGLKKFGALYNF+TL+ P GSLVFSGLIASSIYD EAE+QA+  L               
Sbjct: 487 FGLKKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSL-------------FN 546

Query: 546 TEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSR 592
            +   +C G+IC+FLT +IM GFC IAA LS+ILV RTK VY NLYGK+R
Sbjct: 547 PDDVLRCRGSICYFLTSLIMSGFCLIAAALSMILVQRTKPVYTNLYGKTR 583

BLAST of CsaV3_2G034070 vs. TAIR 10
Match: AT3G01930.1 (Major facilitator superfamily protein )

HSP 1 Score: 621.3 bits (1601), Expect = 8.0e-178
Identity = 326/483 (67.49%), Postives = 386/483 (79.92%), Query Frame = 0

Query: 113 MCALVFIGTNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPE 172
           MC L+F+G NGETYFNT +LVS VQNFPKSRGPVVGILKGFAGL GAIL+Q Y + HS +
Sbjct: 1   MCILIFVGNNGETYFNTAALVSGVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSD 60

Query: 173 SANLIFMVAVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLI 232
            A+LIFMVAV P++V + +MFFIRPV GHRQ+R SD  SFT +Y VC+LLAAYLM VML+
Sbjct: 61  RASLIFMVAVAPSVVVVPLMFFIRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLV 120

Query: 233 EDLVTLSPIVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAE--QEALLPPSEKEEPAR 292
           ED + LS  +I  FTVV+F ILL P FIP++ +  + +T   +  +E LL   + ++P +
Sbjct: 121 EDFIDLSHSIIIAFTVVLFAILLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQ 180

Query: 293 -TEPD-GNEVIFSEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPR 352
            T PD G E+IFSEVEDEK +  DLLPA ER KRIAQLQAKL+QAAAEGAVRVKRR+GP 
Sbjct: 181 STTPDHGPELIFSEVEDEKPKEVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPH 240

Query: 353 RGEDFTLGQALIKADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWN 412
           RGEDFTL QAL+KADFWLIF S LLGSG+GLTVIDNLGQMSQSLGYDNTH+FVS+ISIWN
Sbjct: 241 RGEDFTLTQALVKADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVFVSMISIWN 300

Query: 413 FLGRVGGGYLSEIVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYG 472
           FLGR+GGGY SE++VRD+AYPRP+A+ +AQ++M  GH+F   GWPGAM+IGTL+ GLGYG
Sbjct: 301 FLGRIGGGYFSELIVRDYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYG 360

Query: 473 AHWAIVPATASELFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQ 532
           AHWAIVPATASELFGLKKFGALYNF+TL+ P GSLVFSGLIASSIYD EAE+QA+  L  
Sbjct: 361 AHWAIVPATASELFGLKKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSL-- 420

Query: 533 FQSSSSFWFTRLYTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYG 592
                         +   +C G+IC+FLT +IM GFC IAA LS+ILV RTK VY NLYG
Sbjct: 421 -----------FNPDDVLRCRGSICYFLTSLIMSGFCLIAAALSMILVQRTKPVYTNLYG 470

BLAST of CsaV3_2G034070 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 502.7 bits (1293), Expect = 4.1e-142
Identity = 270/594 (45.45%), Postives = 380/594 (63.97%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLF-GSISPIIKTNLSYNQRQISRLGVA 60
           M  W  KL   +N+RWLVFV A+W+QS AG+GYLF GS+SP IKT+L YNQ+QI+ LGVA
Sbjct: 2   MTLWRHKLELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVA 61

Query: 61  KDLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFI 120
           K+LGD++GF++  L+E+ P W  LLVGA  N  GYG VWL+VTG+ P LPLW +   +F+
Sbjct: 62  KNLGDAIGFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFV 121

Query: 121 GTNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFM 180
           GTNGETY+NT SLVSC+ NFP+SRGPVVGILKGF+GLSGAILTQ Y +F+    +++I M
Sbjct: 122 GTNGETYYNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYLMFNPSHDSSVILM 181

Query: 181 VAVGPALVAIGVMFFIRPV-AGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTL 240
           VA+GP +V + ++F +RPV    R    SD + F ++YG C++LA YL+G+++++ +  +
Sbjct: 182 VALGPPVVVLALLFIVRPVERSCRTNLRSDDLRFLAIYGFCVVLAVYLLGLLVLQSVFDM 241

Query: 241 SPIVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEV 300
           +  +IT    ++ + ++ P  +P S    S                          GN V
Sbjct: 242 TQTIITTSGAILVIFMVVPVLVPFSSVFIS--------------------------GNNV 301

Query: 301 IFSEVED-----EKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDF 360
              + E+     ++ E   L+  S+R                      ++++ P  GEDF
Sbjct: 302 TSVKPEEGTSNVDQHEARTLIERSDRPP--------------------EKKRAPCIGEDF 361

Query: 361 TLGQALIKADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRV 420
           TL QAL +ADFWLIF S +LG G+G+T+IDNLGQ+  SLGY NT IFVSLISI NFLGRV
Sbjct: 362 TLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYSNTKIFVSLISISNFLGRV 421

Query: 421 GGGYLSEIVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAI 480
            GGY SE+++R  + PR +AM++ Q +M  G ++  + WPG +Y+ T++ G+GYGAHWAI
Sbjct: 422 AGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGMGYGAHWAI 481

Query: 481 VPATASELFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSS 540
            PA+ S++FGLK FG+LYNF   + P+GS VFSG+IAS+IYD  A KQA    T+ +S  
Sbjct: 482 APASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQA-GPTTETESL- 534

Query: 541 SFWFTRLYTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLY 588
                         C G++C+ +TC +M   C +A +LSL +V+RT+  Y  L+
Sbjct: 542 -------------VCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFYLRLH 534

BLAST of CsaV3_2G034070 vs. TAIR 10
Match: AT5G50520.1 (Major facilitator superfamily protein )

HSP 1 Score: 502.7 bits (1293), Expect = 4.1e-142
Identity = 270/594 (45.45%), Postives = 380/594 (63.97%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLF-GSISPIIKTNLSYNQRQISRLGVA 60
           M  W  KL   +N+RWLVFV A+W+QS AG+GYLF GS+SP IKT+L YNQ+QI+ LGVA
Sbjct: 2   MTLWRHKLELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVA 61

Query: 61  KDLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFI 120
           K+LGD++GF++  L+E+ P W  LLVGA  N  GYG VWL+VTG+ P LPLW +   +F+
Sbjct: 62  KNLGDAIGFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFV 121

Query: 121 GTNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFM 180
           GTNGETY+NT SLVSC+ NFP+SRGPVVGILKGF+GLSGAILTQ Y +F+    +++I M
Sbjct: 122 GTNGETYYNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYLMFNPSHDSSVILM 181

Query: 181 VAVGPALVAIGVMFFIRPV-AGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTL 240
           VA+GP +V + ++F +RPV    R    SD + F ++YG C++LA YL+G+++++ +  +
Sbjct: 182 VALGPPVVVLALLFIVRPVERSCRTNLRSDDLRFLAIYGFCVVLAVYLLGLLVLQSVFDM 241

Query: 241 SPIVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEV 300
           +  +IT    ++ + ++ P  +P S    S                          GN V
Sbjct: 242 TQTIITTSGAILVIFMVVPVLVPFSSVFIS--------------------------GNNV 301

Query: 301 IFSEVED-----EKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDF 360
              + E+     ++ E   L+  S+R                      ++++ P  GEDF
Sbjct: 302 TSVKPEEGTSNVDQHEARTLIERSDRPP--------------------EKKRAPCIGEDF 361

Query: 361 TLGQALIKADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRV 420
           TL QAL +ADFWLIF S +LG G+G+T+IDNLGQ+  SLGY NT IFVSLISI NFLGRV
Sbjct: 362 TLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYSNTKIFVSLISISNFLGRV 421

Query: 421 GGGYLSEIVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAI 480
            GGY SE+++R  + PR +AM++ Q +M  G ++  + WPG +Y+ T++ G+GYGAHWAI
Sbjct: 422 AGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGMGYGAHWAI 481

Query: 481 VPATASELFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSS 540
            PA+ S++FGLK FG+LYNF   + P+GS VFSG+IAS+IYD  A KQA    T+ +S  
Sbjct: 482 APASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQA-GPTTETESL- 534

Query: 541 SFWFTRLYTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLY 588
                         C G++C+ +TC +M   C +A +LSL +V+RT+  Y  L+
Sbjct: 542 -------------VCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFYLRLH 534

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_011649956.10.0e+00100.00protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Cucumis sativus] >KGN63164.1 hypo... [more]
XP_008441326.10.0e+0096.64PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Cucumis melo] >KAA0056... [more]
XP_038885267.16.8e-30490.10protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Benincasa hispida][more]
XP_023544039.11.9e-29888.42protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo][more]
KAG6602685.14.2e-29888.26Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
F4I9E16.4e-3125.38Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A0A0LQ600.0e+00100.00Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G40613... [more]
A0A5A7UN830.0e+0096.64Protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3B3570.0e+0096.64protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A6J1E5337.8e-29888.09protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111430673... [more]
A0A6J1JLV21.7e-29788.09protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111487... [more]
Match NameE-valueIdentityDescription
AT5G14120.11.1e-23269.27Major facilitator superfamily protein [more]
AT3G01930.23.3e-23269.32Major facilitator superfamily protein [more]
AT3G01930.18.0e-17867.49Major facilitator superfamily protein [more]
AT5G50630.14.1e-14245.45Major facilitator superfamily protein [more]
AT5G50520.14.1e-14245.45Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 316..336
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 4..592
NoneNo IPR availablePANTHERPTHR21576:SF136PROTEIN NUCLEAR FUSION DEFECTIVE 4-LIKEcoord: 4..592
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 15..569
e-value: 1.22973E-106
score: 324.59
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 15..262
e-value: 2.8E-87
score: 292.3
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 364..589
e-value: 5.5E-7
score: 31.3
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 10..202
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 212..582

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_2G034070.1CsaV3_2G034070.1mRNA