CsaV3_2G031680 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_2G031680 vs. NCBI nr
Match: NP_001275523.1 (gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus] >KAE8652189.1 hypothetical protein Csa_021993 [Cucumis sativus] >CCG14217.1 GA 3-oxidase [Cucumis sativus])

HSP 1 Score: 721.5 bits (1861), Expect = 3.6e-204
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0

Query: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60
           MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL
Sbjct: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60

Query: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG
Sbjct: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120

Query: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180
           ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF
Sbjct: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180

Query: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240
           LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN
Sbjct: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240

Query: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300
           NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF
Sbjct: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300

Query: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI
Sbjct: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 350

BLAST of CsaV3_2G031680 vs. NCBI nr
Match: XP_008445138.1 (PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo])

HSP 1 Score: 682.6 bits (1760), Expect = 1.8e-192
Identity = 329/351 (93.73%), Postives = 341/351 (97.15%), Query Frame = 0

Query: 1   MADQEIPPPSQLVPLDFTSVQT-VPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASE 60
           MADQEIPPPSQLVPL+F SVQT VPESHLWPN  ELSAA+KLDKRVSLPLIDLV+D ASE
Sbjct: 1   MADQEIPPPSQLVPLNFASVQTHVPESHLWPNVKELSAAVKLDKRVSLPLIDLVNDDASE 60

Query: 61  LLGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMA 120
           LLGRACEEWGMFQLINHG+S+TLIEEAEEETRRLFTLPA QKMKTLRSPGSLTATGYGMA
Sbjct: 61  LLGRACEEWGMFQLINHGVSQTLIEEAEEETRRLFTLPAAQKMKTLRSPGSLTATGYGMA 120

Query: 121 GISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILK 180
           GISKYHPKLMWHEGFTI+GSPINEAKKLWPNDYKRFCDVMEEYQR+MKGLGERIIRLILK
Sbjct: 121 GISKYHPKLMWHEGFTIIGSPINEAKKLWPNDYKRFCDVMEEYQRQMKGLGERIIRLILK 180

Query: 181 FLGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVG 240
           FLGISEEEMMKLLTSTDESIGKP+MALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQV 
Sbjct: 181 FLGISEEEMMKLLTSTDESIGKPYMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVR 240

Query: 241 NNGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAY 300
           +NGLQIFKDGTGWVPLSPMSGTL+VN+GDLLHILSNGRF SILHRVMIQENKEHRFSLAY
Sbjct: 241 SNGLQIFKDGTGWVPLSPMSGTLVVNVGDLLHILSNGRFTSILHRVMIQENKEHRFSLAY 300

Query: 301 FYDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           FYDPPG+IYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKK G GLPAIKI
Sbjct: 301 FYDPPGDIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKMGSGLPAIKI 351

BLAST of CsaV3_2G031680 vs. NCBI nr
Match: AAF80661.1 (putative gibberellin 3 beta hydroxylase [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 627.1 bits (1616), Expect = 9.2e-176
Identity = 296/350 (84.57%), Postives = 325/350 (92.86%), Query Frame = 0

Query: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60
           MADQE+PPPSQLVPLDF S+QTVPESHLWPN+ ELS AIKLD+R+SLP+ID + DGASEL
Sbjct: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60

Query: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           LGRACE+WGMFQLINHG+ KTLI+EAEEETRRLF+LPA QKMKTLR+  SLTATGYGMAG
Sbjct: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRT-DSLTATGYGMAG 120

Query: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180
           ISKYH KLMWHEGFT+ GSP+++AKKLWPNDYKRFCDVMEEYQ +MKGLGERIIRLI KF
Sbjct: 121 ISKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKF 180

Query: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240
           L ISEEEMMKLLTSTDES GKP+MALRLNSYPPCPDP +VMGLAAHTDTSL TI+HQ  +
Sbjct: 181 LDISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARH 240

Query: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300
           +GLQ+FKDGTGWVPLSPMSGTLIVN+GD+LHILSNGRFPSILHRVMI ENKEHRFSLAYF
Sbjct: 241 DGLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYF 300

Query: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           YDPPG++YISPYCKPLS++PQ PLYR VNVKEYF IKAKK GKGL AIKI
Sbjct: 301 YDPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

BLAST of CsaV3_2G031680 vs. NCBI nr
Match: XP_038885582.1 (gibberellin 3-beta-dioxygenase 1-like [Benincasa hispida])

HSP 1 Score: 597.0 bits (1538), Expect = 1.0e-166
Identity = 277/348 (79.60%), Postives = 317/348 (91.09%), Query Frame = 0

Query: 3   DQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASELLG 62
           DQE+PPPSQ VPLDF+S+QTVPESHLWP++ ELS A++LD+RVSLP+IDL+ +GASELLG
Sbjct: 5   DQELPPPSQFVPLDFSSIQTVPESHLWPDSNELSLAVELDERVSLPIIDLLDNGASELLG 64

Query: 63  RACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGIS 122
           RACEEWGMFQLINHG+ KTLI+EAEEETRRLFTLPA QKMK L+SP S+  TGYGMAGIS
Sbjct: 65  RACEEWGMFQLINHGVPKTLIDEAEEETRRLFTLPARQKMKALQSPSSI--TGYGMAGIS 124

Query: 123 KYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLG 182
           KYHPKLMWHEGFT+LGSP+++AKKLWPNDYKRFCDVMEEYQ +MKGLGER+IRLI KFL 
Sbjct: 125 KYHPKLMWHEGFTVLGSPVDDAKKLWPNDYKRFCDVMEEYQNQMKGLGERLIRLIFKFLH 184

Query: 183 ISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNG 242
           IS++EM+K+LTST +S GKP+MALRLNSYPPCPDP +V+GLAAHTDTSL TI+HQV ++G
Sbjct: 185 ISDKEMVKMLTSTCDSGGKPYMALRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQVRSDG 244

Query: 243 LQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYD 302
           LQ+F DG GWVPLSPMSGTLIVN+GD+LHILSNGRFPSILHRV IQENKEHR+SL YFYD
Sbjct: 245 LQVFNDGAGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVTIQENKEHRYSLGYFYD 304

Query: 303 PPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           PP  +YISPYCKPLS++PQ PLYR VNV+EYF IKA   GKGL AIKI
Sbjct: 305 PPAGLYISPYCKPLSESPQIPLYRCVNVQEYFTIKASNKGKGLSAIKI 350

BLAST of CsaV3_2G031680 vs. NCBI nr
Match: XP_023002882.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita maxima])

HSP 1 Score: 523.5 bits (1347), Expect = 1.4e-144
Identity = 256/350 (73.14%), Postives = 290/350 (82.86%), Query Frame = 0

Query: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60
           MADQE       +PLDF SVQTVPESHLWP++ ELS+ I LD+++SLP IDL+ DGASEL
Sbjct: 1   MADQE------FLPLDFNSVQTVPESHLWPDSEELSSVIDLDEKISLPFIDLLDDGASEL 60

Query: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           LGRACE+WGMFQL NHGISKTLI E EEE RRLFTLPA +KMK LRS   +  TGYG A 
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAEMEEEARRLFTLPAKEKMKALRSADRV--TGYGTAV 120

Query: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180
           ISKYHPK MWHEGFTILGSP N+AKKLWP+D KRFCDVMEEYQ +MKGL +R+IRLI KF
Sbjct: 121 ISKYHPKCMWHEGFTILGSPANDAKKLWPSDSKRFCDVMEEYQSQMKGLSDRLIRLIFKF 180

Query: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240
           L ISEEE MKLL+ST +S GK + AL+LNSYPPCPDP QVMGLAAHTDTSL TI+HQV N
Sbjct: 181 LDISEEETMKLLSSTGDS-GKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQVCN 240

Query: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300
           +GLQIFKD   WVPLSPM G+ +VN+GDLLHI+SNGRFPSILHRV IQ  K HRFSLAYF
Sbjct: 241 DGLQIFKDRASWVPLSPMRGSFVVNVGDLLHIMSNGRFPSILHRVTIQGKKVHRFSLAYF 300

Query: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           Y PP  +YISP+CKPLS++PQ PLYR V+V+EYF +KAK  G+GL  IKI
Sbjct: 301 YYPPPYLYISPHCKPLSESPQAPLYRCVSVEEYFTMKAKNNGEGLSTIKI 341

BLAST of CsaV3_2G031680 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 4.3e-83
Identity = 151/339 (44.54%), Postives = 222/339 (65.49%), Query Frame = 0

Query: 16  DFTSVQTVPESHLWPNATEL----SAAIKLDKRVSLPLIDLVSDGASELLGRACEEWGMF 75
           DF S+Q +PES+ W +  +     S  I  +   ++P+IDL    AS+L+G AC+ WG++
Sbjct: 21  DFNSLQELPESYNWTHLDDHTLIDSNNIMKESTTTVPVIDLNDPNASKLIGLACKTWGVY 80

Query: 76  QLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKLMWH 135
           Q++NHGI  +L+E+ +   + LF+LP+ QK K  RSP  +  +GYG+A IS + PKLMW+
Sbjct: 81  QVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHKATRSPDGV--SGYGIARISSFFPKLMWY 140

Query: 136 EGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGISEEEMMKL 195
           EGFTI+GSP++  ++LWP DY RFCD++ +Y   MK L   ++ L+L  LGI++E++   
Sbjct: 141 EGFTIVGSPLDHFRELWPQDYTRFCDIVVQYDETMKKLAGTLMCLMLDSLGITKEDIK-- 200

Query: 196 LTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQIFKDGTG 255
              +     K   AL+LNSYP CPDP   MGLA HTD++  TIL Q   +GLQ+ ++G+G
Sbjct: 201 WAGSKAQFEKACAALQLNSYPSCPDPDHAMGLAPHTDSTFLTILSQNDISGLQVNREGSG 260

Query: 256 WVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYDPPGEIYISP 315
           W+ + P+ G L+VN+GDL HILSNG +PS+LHRV++   ++ RFS+AY Y PP  + I P
Sbjct: 261 WITVPPLQGGLVVNVGDLFHILSNGLYPSVLHRVLVNRTRQ-RFSVAYLYGPPSNVEICP 320

Query: 316 YCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           + K +  T   PLYRSV   EY   KAK   K L ++++
Sbjct: 321 HAKLIGPTKP-PLYRSVTWNEYLGTKAKHFNKALSSVRL 353

BLAST of CsaV3_2G031680 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 284.3 bits (726), Expect = 1.9e-75
Identity = 148/344 (43.02%), Postives = 213/344 (61.92%), Query Frame = 0

Query: 9   PSQLVPLDFTSVQTVPESHLWPNATEL---SAAIKLDKRVSLPLIDLVSDGASELLGRAC 68
           P   +P DFTS++ +P+S+ W    +L   +A        ++PLIDL    A+  +G AC
Sbjct: 17  PHSHIP-DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDHPDATNQIGHAC 76

Query: 69  EEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYH 128
             WG FQ+ NHG+   L+++ E  T  LF LP  +K+K+ RS   +  +GYG+A I+ + 
Sbjct: 77  RTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGV--SGYGVARIASFF 136

Query: 129 PKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGISE 188
            K MW EGFTI GSP+N+ +KLWP  +  +CD++EEY+  MK L  +++ L L  LG+SE
Sbjct: 137 NKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNSLGVSE 196

Query: 189 EEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQI 248
           E++     S+D  +     AL+LN YP CP+P + MGLAAHTD++L TIL+Q    GLQ+
Sbjct: 197 EDIEWASLSSD--LNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQV 256

Query: 249 FKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYDPPG 308
           F+D  GWV + P  G+L+VN+GDL HILSNG F S+LHR  + + +  R S+A+ + P  
Sbjct: 257 FRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRA-RLSVAFLWGPQS 316

Query: 309 EIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIK 350
           +I ISP  K +S   + PLY+SV  KEY   KA    K L  I+
Sbjct: 317 DIKISPVPKLVSPV-ESPLYQSVTWKEYLRTKATHFNKALSMIR 353

BLAST of CsaV3_2G031680 vs. ExPASy Swiss-Prot
Match: Q9ZT84 (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 278.1 bits (710), Expect = 1.4e-73
Identity = 143/337 (42.43%), Postives = 211/337 (62.61%), Query Frame = 0

Query: 14  PLDFTSVQTVPESHLW-PNATELSAAIKLDKRVSLPLIDLVSDGASELLGRACEEWGMFQ 73
           P DF S   +P+S+ W P    L +A   D+  +LPLIDL     + L+G AC  WG FQ
Sbjct: 21  PPDFKS---LPDSYTWTPKDDLLFSASASDE--TLPLIDLSDIHVATLVGHACTTWGAFQ 80

Query: 74  LINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKLMWHE 133
           + NHG+   L+++ E  T  LF LP  +K+K  RS   +  +GYG+A I+ +  K MW E
Sbjct: 81  ITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGV--SGYGVARIASFFNKKMWSE 140

Query: 134 GFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGISEEEMMKLL 193
           GFT++GSP+++ +KLWP+ + ++C+++EEY+  M+ L  +++   L  LG+ E+++    
Sbjct: 141 GFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLGVEEKDIQWAG 200

Query: 194 TSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQIFKDGTGW 253
            ++D         ++LN YP CP+P + MGLAAHTD++L TIL+Q    GLQ+F+D  GW
Sbjct: 201 PNSD--FQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGW 260

Query: 254 VPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYDPPGEIYISPY 313
           V   P+ G+L+VN+GDLLHIL+NG FPS+LHR  +   +  RFS+AY + PP +I ISP 
Sbjct: 261 VTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRS-RFSMAYLWGPPSDIMISPL 320

Query: 314 CKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIK 350
            K L D  Q PLY S+  K+Y A KA    + L  I+
Sbjct: 321 PK-LVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

BLAST of CsaV3_2G031680 vs. ExPASy Swiss-Prot
Match: Q9SVS8 (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 7.1e-70
Identity = 141/342 (41.23%), Postives = 209/342 (61.11%), Query Frame = 0

Query: 11  QLVPLDFTSVQTVPESHLW--PNATELSAAIKLDKRVSLPLIDLVSDGASELLGRACEEW 70
           +++PLDFT+ +T+P+SH+W  P     S  I        P+I L +     LL +ACEEW
Sbjct: 17  RIIPLDFTNTKTLPDSHVWSKPEPETTSGPI--------PVISLSNPEEHGLLRQACEEW 76

Query: 71  GMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKL 130
           G+F + +HG+S +L+   + + +RLF+LP  +K+  +RSP    +TGYG+  IS ++ KL
Sbjct: 77  GVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDE--STGYGVVRISMFYDKL 136

Query: 131 MWHEGFTILGSPI-NEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGISEEE 190
           MW EGF+++GS +   A  LWP+D+  FC+VMEEYQ+ M  L  R+I +++  LG++ E+
Sbjct: 137 MWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISMLMGSLGLTHED 196

Query: 191 MMKLL-TSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQI- 250
           +  L+   T          L+LNSYP CPDP   MGLA HTD+SL TIL+Q    GL+I 
Sbjct: 197 LGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIE 256

Query: 251 --FKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYDP 310
              ++G+ W+ + P+ G+L+V +GDL HI+SNG+F S +HR ++ +   HR S AYF  P
Sbjct: 257 SPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKT-HHRVSAAYFAGP 316

Query: 311 PGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGL 346
           P  + I P     SD    P+YR +  +EY A KA    K L
Sbjct: 317 PKNLQIGPL---TSDKNHPPIYRRLIWEEYLAAKATHFNKAL 344

BLAST of CsaV3_2G031680 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 6.6e-68
Identity = 138/342 (40.35%), Postives = 208/342 (60.82%), Query Frame = 0

Query: 19  SVQTVPESHLW-----PNATELSAAIKLDK-RVSLPLIDLVSDGASELLGRACEEWGMFQ 78
           S+QT+PES  W      +    S+A+  D    S+P+IDL +   + L+G A + WG FQ
Sbjct: 15  SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74

Query: 79  LINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKLMWHE 138
           + NHGIS+ L+++ E  ++ LF +P+ +K++   S   +  +GYG   IS +  K MW E
Sbjct: 75  IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGV--SGYGEPRISPFFEKKMWSE 134

Query: 139 GFTIL-GSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGISEE--EMM 198
           GFTI   S  N    LWP+D+ ++C +++EY  EM+ L  R++  IL  LG++ E  E  
Sbjct: 135 GFTIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWA 194

Query: 199 KLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQIFKDG 258
             L  +   +G+   A+RLN YP CP+P + MGLAAHTD+++ TILHQ    GLQ+F++ 
Sbjct: 195 HKLEKSGSKVGRG--AIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREE 254

Query: 259 TGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFY-DPPGEIY 318
           +GWV + P  G L+VNIGDL HILSNG+ PS++HR  +   +  R S+AY +  P G++ 
Sbjct: 255 SGWVTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRS-RISIAYLWGGPAGDVQ 314

Query: 319 ISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           I+P  K L+   +  LYRS+  KEY  IK +   K + AI++
Sbjct: 315 IAPISK-LTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

BLAST of CsaV3_2G031680 vs. ExPASy TrEMBL
Match: S0BE47 (GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox4 PE=2 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 1.7e-204
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0

Query: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60
           MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL
Sbjct: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60

Query: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG
Sbjct: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120

Query: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180
           ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF
Sbjct: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180

Query: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240
           LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN
Sbjct: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240

Query: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300
           NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF
Sbjct: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300

Query: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI
Sbjct: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 350

BLAST of CsaV3_2G031680 vs. ExPASy TrEMBL
Match: A0A1S3BBH9 (gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103488263 PE=3 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 8.9e-193
Identity = 329/351 (93.73%), Postives = 341/351 (97.15%), Query Frame = 0

Query: 1   MADQEIPPPSQLVPLDFTSVQT-VPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASE 60
           MADQEIPPPSQLVPL+F SVQT VPESHLWPN  ELSAA+KLDKRVSLPLIDLV+D ASE
Sbjct: 1   MADQEIPPPSQLVPLNFASVQTHVPESHLWPNVKELSAAVKLDKRVSLPLIDLVNDDASE 60

Query: 61  LLGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMA 120
           LLGRACEEWGMFQLINHG+S+TLIEEAEEETRRLFTLPA QKMKTLRSPGSLTATGYGMA
Sbjct: 61  LLGRACEEWGMFQLINHGVSQTLIEEAEEETRRLFTLPAAQKMKTLRSPGSLTATGYGMA 120

Query: 121 GISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILK 180
           GISKYHPKLMWHEGFTI+GSPINEAKKLWPNDYKRFCDVMEEYQR+MKGLGERIIRLILK
Sbjct: 121 GISKYHPKLMWHEGFTIIGSPINEAKKLWPNDYKRFCDVMEEYQRQMKGLGERIIRLILK 180

Query: 181 FLGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVG 240
           FLGISEEEMMKLLTSTDESIGKP+MALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQV 
Sbjct: 181 FLGISEEEMMKLLTSTDESIGKPYMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVR 240

Query: 241 NNGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAY 300
           +NGLQIFKDGTGWVPLSPMSGTL+VN+GDLLHILSNGRF SILHRVMIQENKEHRFSLAY
Sbjct: 241 SNGLQIFKDGTGWVPLSPMSGTLVVNVGDLLHILSNGRFTSILHRVMIQENKEHRFSLAY 300

Query: 301 FYDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           FYDPPG+IYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKK G GLPAIKI
Sbjct: 301 FYDPPGDIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKMGSGLPAIKI 351

BLAST of CsaV3_2G031680 vs. ExPASy TrEMBL
Match: Q9LM06 (Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus OX=3654 GN=WGA3H PE=2 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.5e-176
Identity = 296/350 (84.57%), Postives = 325/350 (92.86%), Query Frame = 0

Query: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60
           MADQE+PPPSQLVPLDF S+QTVPESHLWPN+ ELS AIKLD+R+SLP+ID + DGASEL
Sbjct: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60

Query: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           LGRACE+WGMFQLINHG+ KTLI+EAEEETRRLF+LPA QKMKTLR+  SLTATGYGMAG
Sbjct: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRT-DSLTATGYGMAG 120

Query: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180
           ISKYH KLMWHEGFT+ GSP+++AKKLWPNDYKRFCDVMEEYQ +MKGLGERIIRLI KF
Sbjct: 121 ISKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKF 180

Query: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240
           L ISEEEMMKLLTSTDES GKP+MALRLNSYPPCPDP +VMGLAAHTDTSL TI+HQ  +
Sbjct: 181 LDISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARH 240

Query: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300
           +GLQ+FKDGTGWVPLSPMSGTLIVN+GD+LHILSNGRFPSILHRVMI ENKEHRFSLAYF
Sbjct: 241 DGLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYF 300

Query: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           YDPPG++YISPYCKPLS++PQ PLYR VNVKEYF IKAKK GKGL AIKI
Sbjct: 301 YDPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

BLAST of CsaV3_2G031680 vs. ExPASy TrEMBL
Match: A0A6J1KKU0 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111496619 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 6.9e-145
Identity = 256/350 (73.14%), Postives = 290/350 (82.86%), Query Frame = 0

Query: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60
           MADQE       +PLDF SVQTVPESHLWP++ ELS+ I LD+++SLP IDL+ DGASEL
Sbjct: 1   MADQE------FLPLDFNSVQTVPESHLWPDSEELSSVIDLDEKISLPFIDLLDDGASEL 60

Query: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           LGRACE+WGMFQL NHGISKTLI E EEE RRLFTLPA +KMK LRS   +  TGYG A 
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAEMEEEARRLFTLPAKEKMKALRSADRV--TGYGTAV 120

Query: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180
           ISKYHPK MWHEGFTILGSP N+AKKLWP+D KRFCDVMEEYQ +MKGL +R+IRLI KF
Sbjct: 121 ISKYHPKCMWHEGFTILGSPANDAKKLWPSDSKRFCDVMEEYQSQMKGLSDRLIRLIFKF 180

Query: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240
           L ISEEE MKLL+ST +S GK + AL+LNSYPPCPDP QVMGLAAHTDTSL TI+HQV N
Sbjct: 181 LDISEEETMKLLSSTGDS-GKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQVCN 240

Query: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300
           +GLQIFKD   WVPLSPM G+ +VN+GDLLHI+SNGRFPSILHRV IQ  K HRFSLAYF
Sbjct: 241 DGLQIFKDRASWVPLSPMRGSFVVNVGDLLHIMSNGRFPSILHRVTIQGKKVHRFSLAYF 300

Query: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           Y PP  +YISP+CKPLS++PQ PLYR V+V+EYF +KAK  G+GL  IKI
Sbjct: 301 YYPPPYLYISPHCKPLSESPQAPLYRCVSVEEYFTMKAKNNGEGLSTIKI 341

BLAST of CsaV3_2G031680 vs. ExPASy TrEMBL
Match: A0A6J1GIY0 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111454608 PE=3 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 3.2e-142
Identity = 252/350 (72.00%), Postives = 287/350 (82.00%), Query Frame = 0

Query: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60
           MADQE       +PLDF SVQTVPESHLWP++ ELS+ I LD+++SLP IDL+ DGASEL
Sbjct: 1   MADQE------FLPLDFNSVQTVPESHLWPDSDELSSVIDLDEKISLPSIDLLDDGASEL 60

Query: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           LGRACE+WGMFQL NHGISKTLI E EEE RRLFTLP  +KMKTLRS   +  TGYG A 
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAETEEEARRLFTLPVKEKMKTLRSADRV--TGYGTAV 120

Query: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180
           ISKYHPK MWHEGFTILGSP ++AKKLWP+D KRFCDVMEEYQ EMK L +R+I+LI KF
Sbjct: 121 ISKYHPKCMWHEGFTILGSPADDAKKLWPSDSKRFCDVMEEYQSEMKRLSDRLIKLIFKF 180

Query: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240
           L IS EE MKLL+S D+S GK + AL+LNSYPPCPDP QVMGLAAHTDTSL TI+HQ  N
Sbjct: 181 LDISVEETMKLLSSMDDS-GKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACN 240

Query: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300
           +GLQIFKD  GWVPLSPM G+ +VN+GDLLHI+SNGRF SILHRV IQ  K HRFSLAYF
Sbjct: 241 DGLQIFKDKAGWVPLSPMRGSFVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYF 300

Query: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           Y PP  +YISP+CKPLS++PQ PLYR V+VKEYF +KAK  G+GL  +KI
Sbjct: 301 YYPPPYLYISPHCKPLSESPQTPLYRCVSVKEYFTMKAKNNGEGLSTVKI 341

BLAST of CsaV3_2G031680 vs. TAIR 10
Match: AT1G15550.1 (gibberellin 3-oxidase 1 )

HSP 1 Score: 284.3 bits (726), Expect = 1.4e-76
Identity = 148/344 (43.02%), Postives = 213/344 (61.92%), Query Frame = 0

Query: 9   PSQLVPLDFTSVQTVPESHLWPNATEL---SAAIKLDKRVSLPLIDLVSDGASELLGRAC 68
           P   +P DFTS++ +P+S+ W    +L   +A        ++PLIDL    A+  +G AC
Sbjct: 17  PHSHIP-DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDHPDATNQIGHAC 76

Query: 69  EEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYH 128
             WG FQ+ NHG+   L+++ E  T  LF LP  +K+K+ RS   +  +GYG+A I+ + 
Sbjct: 77  RTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGV--SGYGVARIASFF 136

Query: 129 PKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGISE 188
            K MW EGFTI GSP+N+ +KLWP  +  +CD++EEY+  MK L  +++ L L  LG+SE
Sbjct: 137 NKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNSLGVSE 196

Query: 189 EEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQI 248
           E++     S+D  +     AL+LN YP CP+P + MGLAAHTD++L TIL+Q    GLQ+
Sbjct: 197 EDIEWASLSSD--LNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQV 256

Query: 249 FKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYDPPG 308
           F+D  GWV + P  G+L+VN+GDL HILSNG F S+LHR  + + +  R S+A+ + P  
Sbjct: 257 FRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRA-RLSVAFLWGPQS 316

Query: 309 EIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIK 350
           +I ISP  K +S   + PLY+SV  KEY   KA    K L  I+
Sbjct: 317 DIKISPVPKLVSPV-ESPLYQSVTWKEYLRTKATHFNKALSMIR 353

BLAST of CsaV3_2G031680 vs. TAIR 10
Match: AT1G80340.1 (gibberellin 3-oxidase 2 )

HSP 1 Score: 278.1 bits (710), Expect = 9.8e-75
Identity = 143/337 (42.43%), Postives = 211/337 (62.61%), Query Frame = 0

Query: 14  PLDFTSVQTVPESHLW-PNATELSAAIKLDKRVSLPLIDLVSDGASELLGRACEEWGMFQ 73
           P DF S   +P+S+ W P    L +A   D+  +LPLIDL     + L+G AC  WG FQ
Sbjct: 21  PPDFKS---LPDSYTWTPKDDLLFSASASDE--TLPLIDLSDIHVATLVGHACTTWGAFQ 80

Query: 74  LINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKLMWHE 133
           + NHG+   L+++ E  T  LF LP  +K+K  RS   +  +GYG+A I+ +  K MW E
Sbjct: 81  ITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGV--SGYGVARIASFFNKKMWSE 140

Query: 134 GFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGISEEEMMKLL 193
           GFT++GSP+++ +KLWP+ + ++C+++EEY+  M+ L  +++   L  LG+ E+++    
Sbjct: 141 GFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLGVEEKDIQWAG 200

Query: 194 TSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQIFKDGTGW 253
            ++D         ++LN YP CP+P + MGLAAHTD++L TIL+Q    GLQ+F+D  GW
Sbjct: 201 PNSD--FQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGW 260

Query: 254 VPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYDPPGEIYISPY 313
           V   P+ G+L+VN+GDLLHIL+NG FPS+LHR  +   +  RFS+AY + PP +I ISP 
Sbjct: 261 VTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRS-RFSMAYLWGPPSDIMISPL 320

Query: 314 CKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIK 350
            K L D  Q PLY S+  K+Y A KA    + L  I+
Sbjct: 321 PK-LVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

BLAST of CsaV3_2G031680 vs. TAIR 10
Match: AT4G21690.1 (gibberellin 3-oxidase 3 )

HSP 1 Score: 265.8 bits (678), Expect = 5.0e-71
Identity = 141/342 (41.23%), Postives = 209/342 (61.11%), Query Frame = 0

Query: 11  QLVPLDFTSVQTVPESHLW--PNATELSAAIKLDKRVSLPLIDLVSDGASELLGRACEEW 70
           +++PLDFT+ +T+P+SH+W  P     S  I        P+I L +     LL +ACEEW
Sbjct: 17  RIIPLDFTNTKTLPDSHVWSKPEPETTSGPI--------PVISLSNPEEHGLLRQACEEW 76

Query: 71  GMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKL 130
           G+F + +HG+S +L+   + + +RLF+LP  +K+  +RSP    +TGYG+  IS ++ KL
Sbjct: 77  GVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDE--STGYGVVRISMFYDKL 136

Query: 131 MWHEGFTILGSPI-NEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGISEEE 190
           MW EGF+++GS +   A  LWP+D+  FC+VMEEYQ+ M  L  R+I +++  LG++ E+
Sbjct: 137 MWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISMLMGSLGLTHED 196

Query: 191 MMKLL-TSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQI- 250
           +  L+   T          L+LNSYP CPDP   MGLA HTD+SL TIL+Q    GL+I 
Sbjct: 197 LGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIE 256

Query: 251 --FKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYDP 310
              ++G+ W+ + P+ G+L+V +GDL HI+SNG+F S +HR ++ +   HR S AYF  P
Sbjct: 257 SPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKT-HHRVSAAYFAGP 316

Query: 311 PGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGL 346
           P  + I P     SD    P+YR +  +EY A KA    K L
Sbjct: 317 PKNLQIGPL---TSDKNHPPIYRRLIWEEYLAAKATHFNKAL 344

BLAST of CsaV3_2G031680 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 259.2 bits (661), Expect = 4.7e-69
Identity = 138/342 (40.35%), Postives = 208/342 (60.82%), Query Frame = 0

Query: 19  SVQTVPESHLW-----PNATELSAAIKLDK-RVSLPLIDLVSDGASELLGRACEEWGMFQ 78
           S+QT+PES  W      +    S+A+  D    S+P+IDL +   + L+G A + WG FQ
Sbjct: 15  SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74

Query: 79  LINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKLMWHE 138
           + NHGIS+ L+++ E  ++ LF +P+ +K++   S   +  +GYG   IS +  K MW E
Sbjct: 75  IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGV--SGYGEPRISPFFEKKMWSE 134

Query: 139 GFTIL-GSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGISEE--EMM 198
           GFTI   S  N    LWP+D+ ++C +++EY  EM+ L  R++  IL  LG++ E  E  
Sbjct: 135 GFTIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWA 194

Query: 199 KLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQIFKDG 258
             L  +   +G+   A+RLN YP CP+P + MGLAAHTD+++ TILHQ    GLQ+F++ 
Sbjct: 195 HKLEKSGSKVGRG--AIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREE 254

Query: 259 TGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFY-DPPGEIY 318
           +GWV + P  G L+VNIGDL HILSNG+ PS++HR  +   +  R S+AY +  P G++ 
Sbjct: 255 SGWVTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRS-RISIAYLWGGPAGDVQ 314

Query: 319 ISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 351
           I+P  K L+   +  LYRS+  KEY  IK +   K + AI++
Sbjct: 315 IAPISK-LTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

BLAST of CsaV3_2G031680 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 196.8 bits (499), Expect = 2.9e-50
Identity = 107/276 (38.77%), Postives = 153/276 (55.43%), Query Frame = 0

Query: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           L +ACE+WG FQ+INHGI   ++E+ EE     F +P  +K K    PG  T  GYG A 
Sbjct: 78  LSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMPLEEKKKYPMEPG--TVQGYGQAF 137

Query: 121 ISKYHPKLMWHEGFTI-LGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILK 180
           I     KL W   F + +  P     KLWP+   RF + +E Y +E++ L +R+++ I  
Sbjct: 138 IFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSESLEGYSKEIRELCKRLLKYIAI 197

Query: 181 FLGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVG 240
            LG+ EE         +E  G+   A+R+N YPPC  P  V+GL+ H+D S  T+L Q  
Sbjct: 198 SLGLKEERF-------EEMFGEAVQAVRMNYYPPCSSPDLVLGLSPHSDGSALTVLQQSK 257

Query: 241 NN--GLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSL 300
           N+  GLQI KD T WVP+ P+   L++NIGD + +LSNG++ S+ HR +    KE R ++
Sbjct: 258 NSCVGLQILKDNT-WVPVKPLPNALVINIGDTIEVLSNGKYKSVEHRAVTNREKE-RLTI 317

Query: 301 AYFYDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEY 334
             FY P  E+ I P  + + D      YRS N  +Y
Sbjct: 318 VTFYAPNYEVEIEPMSELVDDETNPCKYRSYNHGDY 342