CsaV3_2G030250 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_2G030250
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionArgininosuccinate lyase
Locationchr2: 19842803 .. 19848017 (+)
RNA-Seq ExpressionCsaV3_2G030250
SyntenyCsaV3_2G030250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTATTACTTGTTTAACATACAAAATTTATTATTCTCTCGTATGTGAAGAGAAATATGAGTTCTGGGAATTTGGGATTTGAAATCAGTCAAACGTTCCCTAATTTCATGCTAAAAGGGAACTAAGAAAGGTGAAAAGGATTTGGAACAAAAAAGTATCTAATAACTTTCCGGTGAGTGATCGCCAGCAGCACTAGGGCTGCAGAACAGAGCCGCCACCTTCTTATAAAAGCCTGACACTATCACTTGCTTCTTTGCTCACTTCACTACGCCATGACAAAACTTGATGTTCAGTCCGCCTCTCAGGCTACCGGAGCAACCTCCGCCGCCTCCAAGCAGCCCGAGGCCAAGGAAATTAAGCTATGGGGCGGCCGCTTCCAGGAGAGCGTAACAGACGCCGTTGAACGCTTTACAGAGTCGATTTCATATGATAAGGACCTTTACAAGCATGACATCCGTGGCAGTCGTGCTCATGCATCCATGCTCGCTAAGCAGGTTCCGTCACTTCTCAGTTTCATTTTGTGATTTTTGTGAAGCTCTGTTCTGTTTACGGAGAGGAATGTTTAATGTTTATTTGTGGCGTTTGTACTGCCCTAATCGAACTGAATCGCGACGATGAGCTGTGGATTTTACTCTAAATACATCTACTATGTTCTGGAAATTTGTCAGTTGCCATGCGCATTCATGATAGTGGTGCGTGGTTCTGGTTGCAGGGATTGATGAGTATCGGTGACAGGGACAGTATTCTAGCAGGTCTCGATGAGATCGAGAGGCGTATTCGAAATGGCGAGTTCGTCTGGAGAGCTGACAGGGAAGATGTTCATATGAACATTGAAGCTGCGCTTACAGATTTGATTGGCGAGCCTGCTAAGAAACTGCATACTGCTCGGAGCCGCAACGACCAGGTAGCGACTGATTTTCGCTTGTGGTGCCGAGATGCTATTGATTCAATAGTTGCGAGGATTAAAGATTTTCAAGTTGCAATGGTAGACTTGGCAGTCAAGAATGCAGGAATTATTGTGCCAGGTTATACACATTTGCAGAGAGCACAGCCCGTCCTACTTCAGCATGTTCTCTTAGCATTCGTTGAGCAGGTACTTAACTATTATTTTGGCTCATCATCTGAAGTTGTGAATGCAATCCTAAAATCAATGGTTTCTTTAGTGCTGCAAAGTGTTAGCTGCATGGTTTCTATGTTCTACCTTACCTGAAACTAGGGATTATAGTCATGAGACTTGGAGAATGTTCGGGGTTGATTTTTAAAATCACCCTAAAATAACATTATAATCATTCATAATTAGTTTAGATGGTATGAAAATCACTTTTAGAAGTGTTTCAAACATTGAATTAATTTTGAATGATTATAGACATGTTTGAGGGTGATTTTGATTCTGATTTTGATTTAGACAAAACTGATTTTATCCGTTCTAAAATCATTCTTAAACGTATTCTTAATAAATGCATCCTTGTCACAAATGATTGTGTATGTATGACTACTTTCACTGCTGAAGCTTTAAACACCTCGCACGAACTTTCAATCCTCTTGATAAAACGTTTTTCACATATTCTAACTTGATATGTTGAGATATTTCTTCATCACATTGATGAATTCTGTTTAGAGACTGATTGATTTGGCTACTTGTACAAAATACAATTCCAGATTAACATTTTAAGAGAACGATTGATTTGTGATGTGCTCAACGGTTATCTTTTTCCTAAGCGTAAATAACTAATCCTAATCTTAAGCTAGGATTAACAATTGCTTGTCCTTTACTCTTAATAAAACTTACAGAAGACTGATTTTGAACAAATGTCATAACATGTTTTGCAGCTCGATAGGGATGCTGGTCGTTTGCTGGACTGCAGAGCTAGACTGAATTTCTGCCCCTTAGGAGCTTGTGCTTTGGCTGGCACTGGCCTTCCAATTGAACGATTCATGACATCAAGTGCTTTAGAATTCTCATCTCCTTTGAGAAACAGGTCTGTTAATTATTTCTACTTGGTGATGTGAATGGCTGGAATATCTTAATTTTCCCTGCTGCAGAGTACAATTTTGGAATTTATTTTTCCTGAGGTTGTGTGGGAGAGCTGGAATAGAAGTTAGGTCGCTTGCATGTTTTGTTTTTGTTTTCTGATTTTGACAATTATGTTTGTTCCCTCACTATTCTTTTGATAAGTTTTTTTTTTCTCCCCATATTTCTTCAAAACACATTTGAACTCTAACCCAAAGTTTTTGAAAACTAAAAAAAAATAGTTTTTAATATCTACTTTTTTTAGTTTTGAAATACATTTTTAAAGAGTAGGTAATAGAGCTAGGAGTAGAGTTTATAACTTTAAATTTAAAAAACATACAACAAAAAACAAAATACTTATAAAATGTGACCTTATTGTTGTTTGCATATGCGATATCAACTATATCTTTATCATCTACAATTCATCTCCACTGATTCTGATGTCACCCTGTTGAATAACTTGTATATTTCTTGTCTCGAATAGTATCGATGCTGTTTCAGACCGAGATTTTGCAATTGAATTTCTTTCTGCCAACTCTATTATGGCTATTCATCTTTCTCGTCTGGGAGAGGAGTGGGTATTGTGGTCCTCTGAGGAGTTCGGATTTATCACACCTAATGACGCTGTTTCAACTGGAAGTAGCATAATGCCTCAGAAGAAAAATCCAGATCCAATGGAACTTGTTCGTGGAAAGTCAGCTAGAGTCATAGGTGATGTAGTAACCCTTCTAACATTGTGCAAAGGACTTCCTCTTGCATACAACCGTGATCTGCAGGTTTGAATCTTTTGAGAAATCTAATTTCCTTTTCAGAACATTTCTTTCACCTTCCATGTGATTCATTAAGCTGTTTGTGTTGAGACAGGAAGATAAGGAACCTGTATTTGATAGTGTCAAGACAGTTTTAGGAATGCTTGAGGTTTCAGCTGAGTTTGCACGAAACATTAGTTTCAATCAGGATAGAATTAAGAAGGCCTTACCTGCGGGTCATCTTGATGCAACCACACTTGCTGACTATCTCGTGCATAAGGTTAGTATATTCTGCCATTTACTTATCCTTCACGGGAAATTTTTGCCACCGACTACAGAGTAATTATTAAATCACCCACATCAATTACCAAGTGCATAACTCATTGACATAAGACAAATAAGACATACATTCTTTACCAAAAAGTTTAAAGATTCTTGAATTTTTCCCTGAACCCCAAATTGTTGTCTAAAAAAATTCACCAATCAACTCAACAGCTAAGGAAAGGACATTACAACAGTTCAAACACAAGATCTCTAATTTCAATATAAGGTAAGATACCAATCAACTCAAAAGTTTAAGCACAACATTTAGTATTTGAAGACAGTTGTCAGAGTAGCTTCTACTTTTGCTTAGAAAGATCGTAATCATACATGCATATGGGGAGGGAGTATGCATGTTCGAGACGTGGTTTAGGTTTTAAATATAAATTTTTGGACCATTTAAGTATGGTGGTAGTTGCTATCATAAAATTGTGGTGCTCAAACTTTACCAATGTTAAAATTGGTCTTTCAAATTTAAATTGGACCCCAAAGTGATAAAAAGTAAACCCTCAAACTTATATCATTTCTACCCTTTCTACAATTATTTAAGTCTATGGGGGTCCAATTTTAATATTTCTATTAGTTTTTTAGGGTATCATTCAAATTTCTGCCCTTAAATTTTTAAAACAATTGATGAGAGCGAACTCTAAAGAATTGACCAGCAAAGATTTTAAAATTTAAAATTCTGAACAAGGAAAAGTGATAGTTAGGAACTCTACAAAATTGAAGCTGACTTTTAACTTGGATAAGAAATCTCAATTCTTTTTCCACCAACTTTTTCATCAATCAATAAAATATGTCTAATCTAAAAAAAAAACAAATAAACATTCTCTATTGGAGAAAAACTAATGGTAAAACATAATGTCCACGGATCTTTTTCCCAGTGAAGCACGTATTTGTTACATTGTTTAGATTAAGAAAAGATTTTCTCAATCTTATCCTTAAGAAAGACCCCGTGGTTGCAACTTTGTAATATGCTTTCATATGAAAATGGGGGCTTCAGATGAGCATCAGGAGAATCATTTACTCCTTCTCACCAAGGTTTAAAGTCTCACTGTTGACAACTGTAGATGAAAGTGTCAAGGTTCCAATTCCTCAAAAATGTTAATGTTAAAAGATATTTTTTTTTTTATAAAAATTCAACTTAGAAATAAATATTGCATGCACTTGTTTCATATTATATTTACATTAATGACATTTCATTACTTATTTTTTATGTTTCAATAAAATGAAAATATTTATTTACTGGCGATGTATAAACATCTATGAAAATGTGAAAACTCTAGTTACAACAACTAGGTGTCGACCCAATGACCGTGTGGTCTACAATTCTCAATGGGTTACTTGACATATTTGGCCGTTGTTTATCACAATGATTTGTAAATCTCAAGAGATAAGACCATGGGTTCAAAATCATTACTCATAACTTTCATGATTTTAGAATAAGAAAAGAACAAGTTTGACTGAATTTTTAATGTTAAGGAGAACAGAATTGTTTCACATTACACATTCAAACACCTTCTAAGTTCTAACTCTAATGATTTCATTTCCTCTTTTTAACAGGGAATTCCTTTTAGAACATCTCATGATATTGTAGGCAAGTCTGTTGCTATGTGCCTTGGAAAGAACTGCCAGCTGAAGGACTTAAGTCTCAATGAACTACGATCTTTGAGTCCAATCTTTGACGAAGATGTTTACGAATTTCTTGGGGTAGAAAATGCAGTGAACAAATTTTCGTCGTATGGCTCCACAGGATCCGAATGTGTTGCCAGTCAACTCCAGTATTGGATTGAAAAGCTTGGCCTTATCAGTCAGTAGAGGGATTAGGGTCTGATGAGTTTTCCCATGGAGTAAGGCTTCAGGCAATGAGTAGAGCAGAATAAAGTAGGAAAGGGGGCGTTTGAAGTTGCTATTTGCAAGCGGAACATTGGTTGCAAAGGGGGTTGTGGGATTGTTATTTAAAGTACTAATTATATTCCTCTAAGCATAAATGTTAGCCATGTGATAAGTACGAATAACTTTTGGGTTTGATTGCATAAATTTGCACGTTATAAGTGTTTCTTGTGACGTTGACAATCTCATCTTAGGAGCTATTCCTTTAAGACCTTGAGATTTATGAGACATGAAAATACCGAA

mRNA sequence

ATGACAAAACTTGATGTTCAGTCCGCCTCTCAGGCTACCGGAGCAACCTCCGCCGCCTCCAAGCAGCCCGAGGCCAAGGAAATTAAGCTATGGGGCGGCCGCTTCCAGGAGAGCGTAACAGACGCCGTTGAACGCTTTACAGAGTCGATTTCATATGATAAGGACCTTTACAAGCATGACATCCGTGGCAGTCGTGCTCATGCATCCATGCTCGCTAAGCAGGGATTGATGAGTATCGGTGACAGGGACAGTATTCTAGCAGGTCTCGATGAGATCGAGAGGCGTATTCGAAATGGCGAGTTCGTCTGGAGAGCTGACAGGGAAGATGTTCATATGAACATTGAAGCTGCGCTTACAGATTTGATTGGCGAGCCTGCTAAGAAACTGCATACTGCTCGGAGCCGCAACGACCAGGTAGCGACTGATTTTCGCTTGTGGTGCCGAGATGCTATTGATTCAATAGTTGCGAGGATTAAAGATTTTCAAGTTGCAATGGTAGACTTGGCAGTCAAGAATGCAGGAATTATTGTGCCAGGTTATACACATTTGCAGAGAGCACAGCCCGTCCTACTTCAGCATGTTCTCTTAGCATTCGTTGAGCAGCTCGATAGGGATGCTGGTCGTTTGCTGGACTGCAGAGCTAGACTGAATTTCTGCCCCTTAGGAGCTTGTGCTTTGGCTGGCACTGGCCTTCCAATTGAACGATTCATGACATCAAGTGCTTTAGAATTCTCATCTCCTTTGAGAAACAGTATCGATGCTGTTTCAGACCGAGATTTTGCAATTGAATTTCTTTCTGCCAACTCTATTATGGCTATTCATCTTTCTCGTCTGGGAGAGGAGTGGGTATTGTGGTCCTCTGAGGAGTTCGGATTTATCACACCTAATGACGCTGTTTCAACTGGAAGTAGCATAATGCCTCAGAAGAAAAATCCAGATCCAATGGAACTTGTTCGTGGAAAGTCAGCTAGAGTCATAGGTGATGTAGTAACCCTTCTAACATTGTGCAAAGGACTTCCTCTTGCATACAACCGTGATCTGCAGGAAGATAAGGAACCTGTATTTGATAGTGTCAAGACAGTTTTAGGAATGCTTGAGGTTTCAGCTGAGTTTGCACGAAACATTAGTTTCAATCAGGATAGAATTAAGAAGGCCTTACCTGCGGGTCATCTTGATGCAACCACACTTGCTGACTATCTCGTGCATAAGGGAATTCCTTTTAGAACATCTCATGATATTGTAGGCAAGTCTGTTGCTATGTGCCTTGGAAAGAACTGCCAGCTGAAGGACTTAAGTCTCAATGAACTACGATCTTTGAGTCCAATCTTTGACGAAGATGTTTACGAATTTCTTGGGGTAGAAAATGCAGTGAACAAATTTTCGTCGTATGGCTCCACAGGATCCGAATGTGTTGCCAGTCAACTCCAGTATTGGATTGAAAAGCTTGGCCTTATCAGTCAGTAG

Coding sequence (CDS)

ATGACAAAACTTGATGTTCAGTCCGCCTCTCAGGCTACCGGAGCAACCTCCGCCGCCTCCAAGCAGCCCGAGGCCAAGGAAATTAAGCTATGGGGCGGCCGCTTCCAGGAGAGCGTAACAGACGCCGTTGAACGCTTTACAGAGTCGATTTCATATGATAAGGACCTTTACAAGCATGACATCCGTGGCAGTCGTGCTCATGCATCCATGCTCGCTAAGCAGGGATTGATGAGTATCGGTGACAGGGACAGTATTCTAGCAGGTCTCGATGAGATCGAGAGGCGTATTCGAAATGGCGAGTTCGTCTGGAGAGCTGACAGGGAAGATGTTCATATGAACATTGAAGCTGCGCTTACAGATTTGATTGGCGAGCCTGCTAAGAAACTGCATACTGCTCGGAGCCGCAACGACCAGGTAGCGACTGATTTTCGCTTGTGGTGCCGAGATGCTATTGATTCAATAGTTGCGAGGATTAAAGATTTTCAAGTTGCAATGGTAGACTTGGCAGTCAAGAATGCAGGAATTATTGTGCCAGGTTATACACATTTGCAGAGAGCACAGCCCGTCCTACTTCAGCATGTTCTCTTAGCATTCGTTGAGCAGCTCGATAGGGATGCTGGTCGTTTGCTGGACTGCAGAGCTAGACTGAATTTCTGCCCCTTAGGAGCTTGTGCTTTGGCTGGCACTGGCCTTCCAATTGAACGATTCATGACATCAAGTGCTTTAGAATTCTCATCTCCTTTGAGAAACAGTATCGATGCTGTTTCAGACCGAGATTTTGCAATTGAATTTCTTTCTGCCAACTCTATTATGGCTATTCATCTTTCTCGTCTGGGAGAGGAGTGGGTATTGTGGTCCTCTGAGGAGTTCGGATTTATCACACCTAATGACGCTGTTTCAACTGGAAGTAGCATAATGCCTCAGAAGAAAAATCCAGATCCAATGGAACTTGTTCGTGGAAAGTCAGCTAGAGTCATAGGTGATGTAGTAACCCTTCTAACATTGTGCAAAGGACTTCCTCTTGCATACAACCGTGATCTGCAGGAAGATAAGGAACCTGTATTTGATAGTGTCAAGACAGTTTTAGGAATGCTTGAGGTTTCAGCTGAGTTTGCACGAAACATTAGTTTCAATCAGGATAGAATTAAGAAGGCCTTACCTGCGGGTCATCTTGATGCAACCACACTTGCTGACTATCTCGTGCATAAGGGAATTCCTTTTAGAACATCTCATGATATTGTAGGCAAGTCTGTTGCTATGTGCCTTGGAAAGAACTGCCAGCTGAAGGACTTAAGTCTCAATGAACTACGATCTTTGAGTCCAATCTTTGACGAAGATGTTTACGAATTTCTTGGGGTAGAAAATGCAGTGAACAAATTTTCGTCGTATGGCTCCACAGGATCCGAATGTGTTGCCAGTCAACTCCAGTATTGGATTGAAAAGCTTGGCCTTATCAGTCAGTAG

Protein sequence

MTKLDVQSASQATGATSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHDIRGSRAHASMLAKQGLMSIGDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGYTHLQRAQPVLLQHVLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSSALEFSSPLRNSIDAVSDRDFAIEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKTVLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGKNCQLKDLSLNELRSLSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWIEKLGLISQ*
Homology
BLAST of CsaV3_2G030250 vs. NCBI nr
Match: XP_004152599.1 (argininosuccinate lyase, chloroplastic [Cucumis sativus] >KGN62752.1 hypothetical protein Csa_022166 [Cucumis sativus])

HSP 1 Score: 964.9 bits (2493), Expect = 2.6e-277
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0

Query: 1   MTKLDVQSASQATGATSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60
           MTKLDVQSASQATGATSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD
Sbjct: 1   MTKLDVQSASQATGATSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60

Query: 61  IRGSRAHASMLAKQGLMSIGDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120
           IRGSRAHASMLAKQGLMSIGDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD
Sbjct: 61  IRGSRAHASMLAKQGLMSIGDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120

Query: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGY 180
           LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGY
Sbjct: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGY 180

Query: 181 THLQRAQPVLLQHVLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSS 240
           THLQRAQPVLLQHVLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSS
Sbjct: 181 THLQRAQPVLLQHVLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSS 240

Query: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300
           ALEFSSPLRNSIDAVSDRDFAIEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVS
Sbjct: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300

Query: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360
           TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT
Sbjct: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360

Query: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420
           VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM
Sbjct: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420

Query: 421 CLGKNCQLKDLSLNELRSLSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWIE 480
           CLGKNCQLKDLSLNELRSLSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWIE
Sbjct: 421 CLGKNCQLKDLSLNELRSLSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWIE 480

Query: 481 KLGLISQ 488
           KLGLISQ
Sbjct: 481 KLGLISQ 487

BLAST of CsaV3_2G030250 vs. NCBI nr
Match: KAA0065095.1 (argininosuccinate lyase [Cucumis melo var. makuwa])

HSP 1 Score: 940.6 bits (2430), Expect = 5.2e-270
Identity = 470/485 (96.91%), Postives = 481/485 (99.18%), Query Frame = 0

Query: 1   MTKLDVQSASQATGATSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60
           MTKLDVQSASQATGA+SAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD
Sbjct: 1   MTKLDVQSASQATGASSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60

Query: 61  IRGSRAHASMLAKQGLMSIGDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120
           IRGSRAHASMLAKQGLMSI DRDSILAGLDEIERRI+NGEFVWRADREDVHMNIEAALTD
Sbjct: 61  IRGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIQNGEFVWRADREDVHMNIEAALTD 120

Query: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGY 180
           LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIV RIKDFQVAMVDLAVKNAGIIVPGY
Sbjct: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDFQVAMVDLAVKNAGIIVPGY 180

Query: 181 THLQRAQPVLLQHVLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSS 240
           THLQRAQPVLLQH+LLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPI+RFMTSS
Sbjct: 181 THLQRAQPVLLQHLLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRFMTSS 240

Query: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300
           ALEFSSPLRNSIDAVSDRDFAIEFLSANS+MAIHLSRLGEEWVLW SEEFGFITPNDAVS
Sbjct: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSVMAIHLSRLGEEWVLWCSEEFGFITPNDAVS 300

Query: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360
           TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT
Sbjct: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360

Query: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420
           VLGMLEVS EFARNISFNQDRIKKALPAGHLDATTLADYLVHKG+PFRTSHDIVGKSVAM
Sbjct: 361 VLGMLEVSTEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGMPFRTSHDIVGKSVAM 420

Query: 421 CLGKNCQLKDLSLNELRSLSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWIE 480
           CLGKNCQLKDLS++ELR+LSPIFD+DVYEFLGVENAVNKFSSYGSTGSECVASQLQYW+E
Sbjct: 421 CLGKNCQLKDLSIDELRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWME 480

Query: 481 KLGLI 486
           KLGLI
Sbjct: 481 KLGLI 485

BLAST of CsaV3_2G030250 vs. NCBI nr
Match: XP_008444922.1 (PREDICTED: argininosuccinate lyase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 939.1 bits (2426), Expect = 1.5e-269
Identity = 469/485 (96.70%), Postives = 480/485 (98.97%), Query Frame = 0

Query: 1   MTKLDVQSASQATGATSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60
           MTKLDVQSASQATGA+SAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD
Sbjct: 1   MTKLDVQSASQATGASSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60

Query: 61  IRGSRAHASMLAKQGLMSIGDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120
           IRGSRAHASMLAKQGLMSI DRDSILAGLDEIERRI+NGEFVWRADREDVHMNIEAALTD
Sbjct: 61  IRGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIQNGEFVWRADREDVHMNIEAALTD 120

Query: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGY 180
           LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIV RIKDFQVAMVDLAVKNAGIIVPGY
Sbjct: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDFQVAMVDLAVKNAGIIVPGY 180

Query: 181 THLQRAQPVLLQHVLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSS 240
           THLQRAQPVLLQH+L AFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPI+RFMTSS
Sbjct: 181 THLQRAQPVLLQHLLFAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRFMTSS 240

Query: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300
           ALEFSSPLRNSIDAVSDRDFAIEFLSANS+MAIHLSRLGEEWVLW SEEFGFITPNDAVS
Sbjct: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSVMAIHLSRLGEEWVLWCSEEFGFITPNDAVS 300

Query: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360
           TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT
Sbjct: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360

Query: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420
           VLGMLEVS EFARNISFNQDRIKKALPAGHLDATTLADYLVHKG+PFRTSHDIVGKSVAM
Sbjct: 361 VLGMLEVSTEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGMPFRTSHDIVGKSVAM 420

Query: 421 CLGKNCQLKDLSLNELRSLSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWIE 480
           CLGKNCQLKDLS++ELR+LSPIFD+DVYEFLGVENAVNKFSSYGSTGSECVASQLQYW+E
Sbjct: 421 CLGKNCQLKDLSIDELRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWME 480

Query: 481 KLGLI 486
           KLGLI
Sbjct: 481 KLGLI 485

BLAST of CsaV3_2G030250 vs. NCBI nr
Match: XP_038886091.1 (argininosuccinate lyase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 929.5 bits (2401), Expect = 1.2e-266
Identity = 467/491 (95.11%), Postives = 481/491 (97.96%), Query Frame = 0

Query: 1   MTKLDVQSASQATGATSA----ASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDL 60
           MTKLDV SASQ TGATSA    A+K+P AKEIKLWGGRFQESVTDAVERFTESISYDKDL
Sbjct: 1   MTKLDVPSASQPTGATSAAGSCATKKPGAKEIKLWGGRFQESVTDAVERFTESISYDKDL 60

Query: 61  YKHDIRGSRAHASMLAKQGLMSIGDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120
           YKHDI GSRAHASMLAKQGLMSI DRD+ILAGLDEIERRIRNGEFVWRADREDVHMNIEA
Sbjct: 61  YKHDISGSRAHASMLAKQGLMSISDRDNILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120

Query: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVARIKDFQVAMVDLAVKNAGII 180
           ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIV RIKDFQVAMVDLA+KNAGII
Sbjct: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDFQVAMVDLAIKNAGII 180

Query: 181 VPGYTHLQRAQPVLLQHVLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIERF 240
           VPGYTHLQRAQPVLLQH+LLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPI+RF
Sbjct: 181 VPGYTHLQRAQPVLLQHLLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRF 240

Query: 241 MTSSALEFSSPLRNSIDAVSDRDFAIEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPN 300
           MTSSALEFSSPLRNSIDAVSDRDFA+EFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPN
Sbjct: 241 MTSSALEFSSPLRNSIDAVSDRDFAMEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPN 300

Query: 301 DAVSTGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFD 360
           DAVSTGSSIMPQKKNPDPMELVRGKSARVIGD+VTLLTLCKGLPLAYNRDLQEDKEPVFD
Sbjct: 301 DAVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360

Query: 361 SVKTVLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420
           SVKTVLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK
Sbjct: 361 SVKTVLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420

Query: 421 SVAMCLGKNCQLKDLSLNELRSLSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLQ 480
           SVAMCLGKNCQLKDLSL+ELR+LSPIFD+DVYEFLGVEN+VNKFSSYGSTGSECVA+QLQ
Sbjct: 421 SVAMCLGKNCQLKDLSLDELRTLSPIFDDDVYEFLGVENSVNKFSSYGSTGSECVANQLQ 480

Query: 481 YWIEKLGLISQ 488
           YWIEKLG+ISQ
Sbjct: 481 YWIEKLGIISQ 491

BLAST of CsaV3_2G030250 vs. NCBI nr
Match: XP_023546913.1 (argininosuccinate lyase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 911.4 bits (2354), Expect = 3.4e-261
Identity = 454/484 (93.80%), Postives = 471/484 (97.31%), Query Frame = 0

Query: 1   MTKLDVQSASQATGATSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60
           MT  DV SA++ATG TSAA+K+PEAKEIKLWGGRFQESVTDAVERFTESISYDKDL+KHD
Sbjct: 1   MTIFDVSSAAEATGETSAAAKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLFKHD 60

Query: 61  IRGSRAHASMLAKQGLMSIGDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120
           I GSRAHASMLAKQGLMSI DRD ILAGLDEIE+RIRNGEFVWRADREDVHMNIEAALTD
Sbjct: 61  ISGSRAHASMLAKQGLMSISDRDCILAGLDEIEKRIRNGEFVWRADREDVHMNIEAALTD 120

Query: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGY 180
           LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSI+ RIKDFQVAMVDLA+KN GIIVPGY
Sbjct: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIIERIKDFQVAMVDLAIKNKGIIVPGY 180

Query: 181 THLQRAQPVLLQHVLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSS 240
           THLQRAQPVLLQH+LLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPI+RFMTSS
Sbjct: 181 THLQRAQPVLLQHLLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRFMTSS 240

Query: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300
           AL FSSPLRNSIDAVSDRDFA+EFLSANSIMAIHLSRLGEEWVLWSSEEFGFI P+DAVS
Sbjct: 241 ALGFSSPLRNSIDAVSDRDFAMEFLSANSIMAIHLSRLGEEWVLWSSEEFGFIVPSDAVS 300

Query: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360
           TGSSIMPQKKNPDPMELVRGKSARVIGD+VTLL+LCKGLPLAYNRDLQEDKEPVFDSVKT
Sbjct: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDLVTLLSLCKGLPLAYNRDLQEDKEPVFDSVKT 360

Query: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420
           V GMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM
Sbjct: 361 VSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420

Query: 421 CLGKNCQLKDLSLNELRSLSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWIE 480
           CLGKNCQLKDLSL ELR+LSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQL+YWIE
Sbjct: 421 CLGKNCQLKDLSLAELRALSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLEYWIE 480

Query: 481 KLGL 485
           KLG+
Sbjct: 481 KLGI 484

BLAST of CsaV3_2G030250 vs. ExPASy Swiss-Prot
Match: Q9LEU8 (Argininosuccinate lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g10920 PE=2 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 2.9e-215
Identity = 354/462 (76.62%), Postives = 416/462 (90.04%), Query Frame = 0

Query: 25  AKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHDIRGSRAHASMLAKQGLMSIGDRDS 84
           +KE+KLWGGRF+ESVT+ VE+FTESIS+DK LYK DI GS+AHASMLA QGL++  D+DS
Sbjct: 55  SKEVKLWGGRFEESVTEKVEKFTESISFDKVLYKQDIMGSKAHASMLAHQGLITDSDKDS 114

Query: 85  ILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFR 144
           IL GLD+IER+I   +F WR DREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFR
Sbjct: 115 ILRGLDDIERQIEANKFEWRTDREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFR 174

Query: 145 LWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGYTHLQRAQPVLLQHVLLAFVEQLDR 204
           LWCRDAID+I+ +I++ Q A+V+LA+KN  +IVPGYTHLQRAQPVLL HVLL FVEQL+R
Sbjct: 175 LWCRDAIDTIIVKIRNLQRALVELALKNEALIVPGYTHLQRAQPVLLPHVLLTFVEQLER 234

Query: 205 DAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSSALEFSSPLRNSIDAVSDRDFAIEF 264
           DAGR +DCRARLNF PLGACALAGTGLPI+RFMT++AL F+ P+RNSIDAVSDRDF +EF
Sbjct: 235 DAGRYVDCRARLNFSPLGACALAGTGLPIDRFMTANALGFTEPMRNSIDAVSDRDFVLEF 294

Query: 265 LSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGKSAR 324
           L  N+   IHLSRLGEEWVLW+SEEFGF+TP+D+VSTGSSIMPQKKNPDPMELVRGKSAR
Sbjct: 295 LYTNANTGIHLSRLGEEWVLWASEEFGFMTPSDSVSTGSSIMPQKKNPDPMELVRGKSAR 354

Query: 325 VIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKTVLGMLEVSAEFARNISFNQDRIKK 384
           VIGD+VT+LTLCKGLPLAYNRD QEDKEP+FDS KT++GM++VSAEFA+N++FN+DRIKK
Sbjct: 355 VIGDLVTVLTLCKGLPLAYNRDFQEDKEPMFDSTKTIMGMIDVSAEFAQNVTFNEDRIKK 414

Query: 385 ALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGKNCQLKDLSLNELRSLSPIFD 444
           +LPAGHLDATTLADYLV KG+PFR+SHDIVGK V +C+ K C+L++LSL E++ LSP+F+
Sbjct: 415 SLPAGHLDATTLADYLVKKGMPFRSSHDIVGKLVGVCVSKGCELQNLSLEEMKKLSPVFE 474

Query: 445 EDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWIEKLGLIS 487
           EDV+ FLGVEN+VNKFSSYGSTGS CVA QL YW+ KL + S
Sbjct: 475 EDVFGFLGVENSVNKFSSYGSTGSNCVAEQLGYWVNKLNITS 516

BLAST of CsaV3_2G030250 vs. ExPASy Swiss-Prot
Match: Q6AR60 (Argininosuccinate lyase OS=Desulfotalea psychrophila (strain LSv54 / DSM 12343) OX=177439 GN=argH PE=3 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 4.9e-130
Identity = 230/444 (51.80%), Postives = 302/444 (68.02%), Query Frame = 0

Query: 24  EAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHDIRGSRAHASMLAKQGLMSIGDRD 83
           E K  KLWGGRF E++  +VE+F+ESISYD  LYK+DI GS+AHA+ML+ QG++S  + +
Sbjct: 3   EQKSKKLWGGRFSEAMAASVEKFSESISYDVRLYKYDIAGSKAHATMLSSQGIISPEELE 62

Query: 84  SILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDF 143
            I+AGL  IE  I  G F ++ + EDVHMNIE AL D IG    +LH ARSRNDQ+A DF
Sbjct: 63  QIIAGLSSIEADIEAGVFEFKTEYEDVHMNIEQALVDRIGAAGSRLHAARSRNDQIALDF 122

Query: 144 RLWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGYTHLQRAQPVLLQHVLLAFVEQLD 203
           +++ RD  D +V  +     A   +  K  G I+PGYTH QRAQPVL+ H +LA+ E   
Sbjct: 123 KMYLRDQCDHLVELLDGACRAFTVVGRKYLGDIMPGYTHTQRAQPVLITHHMLAYYEMFR 182

Query: 204 RDAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSSALEFSSPLRNSIDAVSDRDFAIE 263
           RD  R+LDCR RLN  PLG  A+AGTGLPI R   + AL F+    NS+D  +DRD+AIE
Sbjct: 183 RDRDRILDCRKRLNLSPLGCAAMAGTGLPINREQVAKALGFAGVTANSMDTSADRDYAIE 242

Query: 264 FLSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGKSA 323
             S  +++ +HLSRL EE V WS+ E+ F+  +D+  TGSSIMPQKKNPD  EL+RGKS 
Sbjct: 243 LTSCLTMIQLHLSRLAEELVTWSTSEYKFVDISDSFCTGSSIMPQKKNPDIAELIRGKSG 302

Query: 324 RVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKTVLGMLEVSAEFARNISFNQDRIK 383
           RV+G +++L+T+ KGLPL YNRD QEDKEPVFD++ TV   L ++AE   ++ FN  R  
Sbjct: 303 RVVGSLISLITMMKGLPLTYNRDQQEDKEPVFDAIDTVSASLSITAEMMAHMKFNTARCA 362

Query: 384 KALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGKNCQLKDLSLNELRSLSPIF 443
           +A   G + AT LADYLV K +PFR +H IVG +VA C+ K C+L DL+L E++  SP+ 
Sbjct: 363 EATETGFITATDLADYLVMKDVPFRQAHHIVGSAVAACIAKGCELPDLTLTEMQEFSPVI 422

Query: 444 DEDVYEFLGVENAVNKFSSYGSTG 468
           + DV+  L  E +VN   S G TG
Sbjct: 423 ESDVFAVLTAEGSVNSRVSTGGTG 446

BLAST of CsaV3_2G030250 vs. ExPASy Swiss-Prot
Match: Q3JDS2 (Argininosuccinate lyase OS=Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / NCIMB 11848 / C-107) OX=323261 GN=argH PE=3 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 4.2e-129
Identity = 241/458 (52.62%), Postives = 308/458 (67.25%), Query Frame = 0

Query: 29  KLWGGRFQESVTDAVERFTESISYDKDLYKHDIRGSRAHASMLAKQGLMSIGDRDSILAG 88
           K W GRF E     VE FT SI +D  LY+ DI GS AHA MLAK G++S  + D+I+ G
Sbjct: 10  KPWSGRFHEPTDTFVEEFTASIGFDHRLYRQDIHGSIAHARMLAKVGVLSREECDAIIQG 69

Query: 89  LDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFRLWCR 148
           L+ I + I  G+F W    EDVHMNIEAALT+ IG   KKLHT RSRNDQ+ATD RL+ R
Sbjct: 70  LETIAQDISRGQFSWSVALEDVHMNIEAALTERIGPVGKKLHTGRSRNDQIATDIRLYLR 129

Query: 149 DAIDSIVARIKDFQVAMVDLAVKNAGIIVPGYTHLQRAQPVLLQHVLLAFVEQLDRDAGR 208
           DAID I A+++  Q  ++ +A + A  I+PG+THLQ AQPV   H L+A+ E L RD GR
Sbjct: 130 DAIDLITAQLQRLQEGLLAIAEREAATIMPGFTHLQTAQPVTFGHHLMAWFEMLLRDTGR 189

Query: 209 LLDCRARLNFCPLGACALAGTGLPIERFMTSSALEFSSPLRNSIDAVSDRDFAIEFLSAN 268
           L DCR R+N  PLGA ALAGT  PI+R  T+  L F     NS+DAVSDRDFAIEF +A 
Sbjct: 190 LADCRHRVNVLPLGAAALAGTTFPIDRAYTAELLGFDGIAENSLDAVSDRDFAIEFTAAG 249

Query: 269 SIMAIHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGKSARVIGD 328
           +++  HLSR  EE VLWSS +F FIT  D   TGSSIMPQKKNPD  ELVRGK+ RV G 
Sbjct: 250 ALLMTHLSRFAEELVLWSSAQFDFITLPDRFCTGSSIMPQKKNPDVPELVRGKTGRVNGH 309

Query: 329 VVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKTVLGMLEVSAEFARNISFNQDRIKKALPA 388
           +V+LLTL KG PLAYN+D QEDKEP+FD+V T+LG L   A+    I+ N D++++A   
Sbjct: 310 LVSLLTLMKGQPLAYNKDNQEDKEPLFDTVDTLLGCLRAFADMIPAITTNPDKMREAATK 369

Query: 389 GHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGKNCQLKDLSLNELRSLSPIFDEDVY 448
           G+  AT LADYLV KG+ FR +H+IVGK+VA+ + ++  L  L L  L+  SP+ +EDV+
Sbjct: 370 GYATATDLADYLVRKGVSFRDAHEIVGKAVALAIEQSKDLAALKLATLQDFSPVIEEDVF 429

Query: 449 EFLGVENAVNKFSSYGSTGSECVASQLQYWIEKLGLIS 487
           + L +E +V   +  G T    V + +Q   EKL  ++
Sbjct: 430 DVLTLEGSVAARNHLGGTAPAQVRAAIQRGREKLNSLA 467

BLAST of CsaV3_2G030250 vs. ExPASy Swiss-Prot
Match: Q2SQ67 (Argininosuccinate lyase OS=Hahella chejuensis (strain KCTC 2396) OX=349521 GN=argH PE=3 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 9.3e-129
Identity = 239/456 (52.41%), Postives = 302/456 (66.23%), Query Frame = 0

Query: 29  KLWGGRFQESVTDAVERFTESISYDKDLYKHDIRGSRAHASMLAKQGLMSIGDRDSILAG 88
           KLWGGRF E+    VERFT S+ +D+ +Y HDIRGS AHA+MLAK G+++  +RD I+ G
Sbjct: 9   KLWGGRFTEATDAFVERFTASVDFDRRMYHHDIRGSIAHATMLAKVGVLTEEERDQIING 68

Query: 89  LDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFRLWCR 148
           L EIE  I  G F W    EDVHMNIEA LT  IG   KKLHT RSRNDQVATD RL+ R
Sbjct: 69  LREIEAEIEAGAFEWSVKLEDVHMNIEARLTQKIGITGKKLHTGRSRNDQVATDIRLYLR 128

Query: 149 DAIDSIVARIKDFQVAMVDLAVKNAGIIVPGYTHLQRAQPVLLQHVLLAFVEQLDRDAGR 208
           D +D I A +     A+ DLA + A  I+PG+THLQ AQPV   H +LA+ E L RD  R
Sbjct: 129 DEVDVIAAELLRLIAALADLAEREADAIMPGFTHLQTAQPVTFGHHMLAWAEMLKRDFSR 188

Query: 209 LLDCRARLNFCPLGACALAGTGLPIERFMTSSALEFSSPLRNSIDAVSDRDFAIEFLSAN 268
           L DCR R N  PLGA ALAGT   I+R  TS  L F+ P  NS+DAVSDRDFAIEF S  
Sbjct: 189 LADCRERFNVSPLGAAALAGTTYSIDRQYTSELLGFNGPAENSLDAVSDRDFAIEFCSFA 248

Query: 269 SIMAIHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGKSARVIGD 328
           S++ +HLSR  EE VLW+S +F FI   D   TGSSIMPQKKNPD  EL+RGKS RV G 
Sbjct: 249 SLLMMHLSRFSEELVLWTSAQFNFINLPDRFCTGSSIMPQKKNPDVPELIRGKSGRVSGH 308

Query: 329 VVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKTVLGMLEVSAEFARNISFNQDRIKKALPA 388
           +++LL L K  PLAYN+D QEDKEP+FD++ TV G L+   +    +S N+D + ++   
Sbjct: 309 LISLLMLMKSQPLAYNKDNQEDKEPLFDAIDTVKGCLKAYGDMMPAVSVNRDAMAESARR 368

Query: 389 GHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGKNCQLKDLSLNELRSLSPIFDEDVY 448
           G   AT LADYLV KG+PFR +H++VGK+VA+ +     L ++SL ELR  S   + DV+
Sbjct: 369 GFSTATDLADYLVRKGLPFRDAHEVVGKAVALGVSSGRDLSEMSLEELREFSADIEVDVF 428

Query: 449 EFLGVENAVNKFSSYGSTGSECVAS---QLQYWIEK 482
           E L +E +VN  +  G T  E V +   + + W+++
Sbjct: 429 EVLTLEGSVNARNHIGGTAPEQVRAAVVRTREWLKR 464

BLAST of CsaV3_2G030250 vs. ExPASy Swiss-Prot
Match: Q5P7H3 (Argininosuccinate lyase OS=Aromatoleum aromaticum (strain EbN1) OX=76114 GN=argH PE=3 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 1.6e-128
Identity = 238/460 (51.74%), Postives = 299/460 (65.00%), Query Frame = 0

Query: 16  TSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHDIRGSRAHASMLAKQG 75
           T  +S QP     K W GRF E V+D V+R+T S+S+D+ + + DIRGS AHA MLA+QG
Sbjct: 2   TDTSSSQP----AKAWSGRFSEPVSDLVKRYTASVSFDQRMAQQDIRGSLAHAKMLARQG 61

Query: 76  LMSIGDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSR 135
           ++   D   I  G+ +I   I  GEF W  D EDVH+NIE  LT L+G P K+LHT RSR
Sbjct: 62  IIGATDLADIERGMVQIRGEIERGEFAWNLDDEDVHLNIEKRLTALVGNPGKRLHTGRSR 121

Query: 136 NDQVATDFRLWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGYTHLQRAQPVLLQHVL 195
           NDQVATD RLW RDAID I+A I +FQ  ++D+A  NA   +PG+THLQ AQPV   H L
Sbjct: 122 NDQVATDIRLWLRDAIDRILALIGEFQKNLLDVAEANAATPMPGFTHLQVAQPVTFGHHL 181

Query: 196 LAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSSALEFSSPLRNSIDAV 255
           +A+ E   RDA R  DCR R+N  PLGA ALAGT  PI+R   +  L F     NS+DAV
Sbjct: 182 MAYFEMSRRDAERFTDCRKRVNRLPLGAAALAGTSYPIDREFVARELGFDEVCHNSLDAV 241

Query: 256 SDRDFAIEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPM 315
           SDRDFAIEF +A++++  HLSRL EE +LW S   GFI   D   TGSSIMPQKKNPD  
Sbjct: 242 SDRDFAIEFCAASALLMTHLSRLSEELILWMSPRVGFIDLADRFCTGSSIMPQKKNPDVP 301

Query: 316 ELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKTVLGMLEVSAEFARNI 375
           ELVRGK+ RV G ++ LLTL KG PLAYN+D QEDKEP+FD+  TV+  L + A+    I
Sbjct: 302 ELVRGKTGRVNGSLIALLTLMKGQPLAYNKDNQEDKEPLFDTADTVIDTLRIYADMITGI 361

Query: 376 SFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGKNCQLKDLSLNE 435
               D ++ AL  G+  AT LADYLV KG+PFR +H+ V  +V     K C L D SL+E
Sbjct: 362 RVKADAMRDALKQGYATATDLADYLVKKGLPFRDAHEAVALAVRAAEAKGCDLPDFSLDE 421

Query: 436 LRSLSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQL 476
           LR+ SP+  EDV+  L VE ++   +  G T  E V + +
Sbjct: 422 LRAFSPLVGEDVFAVLTVEGSLASRAHVGGTAPEQVRAAI 457

BLAST of CsaV3_2G030250 vs. ExPASy TrEMBL
Match: A0A0A0LPM3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G370510 PE=3 SV=1)

HSP 1 Score: 964.9 bits (2493), Expect = 1.3e-277
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0

Query: 1   MTKLDVQSASQATGATSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60
           MTKLDVQSASQATGATSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD
Sbjct: 1   MTKLDVQSASQATGATSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60

Query: 61  IRGSRAHASMLAKQGLMSIGDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120
           IRGSRAHASMLAKQGLMSIGDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD
Sbjct: 61  IRGSRAHASMLAKQGLMSIGDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120

Query: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGY 180
           LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGY
Sbjct: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGY 180

Query: 181 THLQRAQPVLLQHVLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSS 240
           THLQRAQPVLLQHVLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSS
Sbjct: 181 THLQRAQPVLLQHVLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSS 240

Query: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300
           ALEFSSPLRNSIDAVSDRDFAIEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVS
Sbjct: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300

Query: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360
           TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT
Sbjct: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360

Query: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420
           VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM
Sbjct: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420

Query: 421 CLGKNCQLKDLSLNELRSLSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWIE 480
           CLGKNCQLKDLSLNELRSLSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWIE
Sbjct: 421 CLGKNCQLKDLSLNELRSLSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWIE 480

Query: 481 KLGLISQ 488
           KLGLISQ
Sbjct: 481 KLGLISQ 487

BLAST of CsaV3_2G030250 vs. ExPASy TrEMBL
Match: A0A5A7VGX5 (Argininosuccinate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004280 PE=3 SV=1)

HSP 1 Score: 940.6 bits (2430), Expect = 2.5e-270
Identity = 470/485 (96.91%), Postives = 481/485 (99.18%), Query Frame = 0

Query: 1   MTKLDVQSASQATGATSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60
           MTKLDVQSASQATGA+SAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD
Sbjct: 1   MTKLDVQSASQATGASSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60

Query: 61  IRGSRAHASMLAKQGLMSIGDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120
           IRGSRAHASMLAKQGLMSI DRDSILAGLDEIERRI+NGEFVWRADREDVHMNIEAALTD
Sbjct: 61  IRGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIQNGEFVWRADREDVHMNIEAALTD 120

Query: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGY 180
           LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIV RIKDFQVAMVDLAVKNAGIIVPGY
Sbjct: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDFQVAMVDLAVKNAGIIVPGY 180

Query: 181 THLQRAQPVLLQHVLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSS 240
           THLQRAQPVLLQH+LLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPI+RFMTSS
Sbjct: 181 THLQRAQPVLLQHLLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRFMTSS 240

Query: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300
           ALEFSSPLRNSIDAVSDRDFAIEFLSANS+MAIHLSRLGEEWVLW SEEFGFITPNDAVS
Sbjct: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSVMAIHLSRLGEEWVLWCSEEFGFITPNDAVS 300

Query: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360
           TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT
Sbjct: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360

Query: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420
           VLGMLEVS EFARNISFNQDRIKKALPAGHLDATTLADYLVHKG+PFRTSHDIVGKSVAM
Sbjct: 361 VLGMLEVSTEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGMPFRTSHDIVGKSVAM 420

Query: 421 CLGKNCQLKDLSLNELRSLSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWIE 480
           CLGKNCQLKDLS++ELR+LSPIFD+DVYEFLGVENAVNKFSSYGSTGSECVASQLQYW+E
Sbjct: 421 CLGKNCQLKDLSIDELRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWME 480

Query: 481 KLGLI 486
           KLGLI
Sbjct: 481 KLGLI 485

BLAST of CsaV3_2G030250 vs. ExPASy TrEMBL
Match: A0A1S3BBH8 (argininosuccinate lyase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103488122 PE=3 SV=1)

HSP 1 Score: 939.1 bits (2426), Expect = 7.4e-270
Identity = 469/485 (96.70%), Postives = 480/485 (98.97%), Query Frame = 0

Query: 1   MTKLDVQSASQATGATSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60
           MTKLDVQSASQATGA+SAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD
Sbjct: 1   MTKLDVQSASQATGASSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60

Query: 61  IRGSRAHASMLAKQGLMSIGDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120
           IRGSRAHASMLAKQGLMSI DRDSILAGLDEIERRI+NGEFVWRADREDVHMNIEAALTD
Sbjct: 61  IRGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIQNGEFVWRADREDVHMNIEAALTD 120

Query: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGY 180
           LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIV RIKDFQVAMVDLAVKNAGIIVPGY
Sbjct: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDFQVAMVDLAVKNAGIIVPGY 180

Query: 181 THLQRAQPVLLQHVLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSS 240
           THLQRAQPVLLQH+L AFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPI+RFMTSS
Sbjct: 181 THLQRAQPVLLQHLLFAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRFMTSS 240

Query: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300
           ALEFSSPLRNSIDAVSDRDFAIEFLSANS+MAIHLSRLGEEWVLW SEEFGFITPNDAVS
Sbjct: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSVMAIHLSRLGEEWVLWCSEEFGFITPNDAVS 300

Query: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360
           TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT
Sbjct: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360

Query: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420
           VLGMLEVS EFARNISFNQDRIKKALPAGHLDATTLADYLVHKG+PFRTSHDIVGKSVAM
Sbjct: 361 VLGMLEVSTEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGMPFRTSHDIVGKSVAM 420

Query: 421 CLGKNCQLKDLSLNELRSLSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWIE 480
           CLGKNCQLKDLS++ELR+LSPIFD+DVYEFLGVENAVNKFSSYGSTGSECVASQLQYW+E
Sbjct: 421 CLGKNCQLKDLSIDELRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWME 480

Query: 481 KLGLI 486
           KLGLI
Sbjct: 481 KLGLI 485

BLAST of CsaV3_2G030250 vs. ExPASy TrEMBL
Match: A0A6J1HD89 (argininosuccinate lyase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111462454 PE=3 SV=1)

HSP 1 Score: 908.3 bits (2346), Expect = 1.4e-260
Identity = 452/484 (93.39%), Postives = 470/484 (97.11%), Query Frame = 0

Query: 1   MTKLDVQSASQATGATSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60
           MT  DV SA++ATG TSAA+K+PEAKEIKLWGGRFQESVTDAVERFTESISYDKDL+KHD
Sbjct: 1   MTIFDVSSAAEATGETSAAAKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLFKHD 60

Query: 61  IRGSRAHASMLAKQGLMSIGDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120
           I GSRAHASMLAKQGLMSI DRD ILAGLDEIE+RI NGEFVWRADREDVHMNIEAALTD
Sbjct: 61  ISGSRAHASMLAKQGLMSISDRDCILAGLDEIEKRILNGEFVWRADREDVHMNIEAALTD 120

Query: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGY 180
           LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSI+ RIKDFQVAMVDLA+KN GIIVPGY
Sbjct: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIIERIKDFQVAMVDLAIKNKGIIVPGY 180

Query: 181 THLQRAQPVLLQHVLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSS 240
           THLQRAQPVLLQH+LLAF+EQLDRDAGRLLDCRARLNFCPLGACALAGTGLPI+RFMTSS
Sbjct: 181 THLQRAQPVLLQHLLLAFIEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRFMTSS 240

Query: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300
           AL FSSPLRNSIDAVSDRDFA+EFLSANSIMAIHLSRLGEEWVLWSSEEFGFI P+DAVS
Sbjct: 241 ALGFSSPLRNSIDAVSDRDFAMEFLSANSIMAIHLSRLGEEWVLWSSEEFGFIVPSDAVS 300

Query: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360
           TGSSIMPQKKNPDPMELVRGKSARVIGD+VTLL+LCKGLPLAYNRDLQEDKEPVFDSVKT
Sbjct: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDLVTLLSLCKGLPLAYNRDLQEDKEPVFDSVKT 360

Query: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420
           V GMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM
Sbjct: 361 VSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420

Query: 421 CLGKNCQLKDLSLNELRSLSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWIE 480
           CLGKNCQLKDLSL ELR+LSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQL+YWIE
Sbjct: 421 CLGKNCQLKDLSLAELRALSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLEYWIE 480

Query: 481 KLGL 485
           KLG+
Sbjct: 481 KLGI 484

BLAST of CsaV3_2G030250 vs. ExPASy TrEMBL
Match: A0A6J1K8P1 (argininosuccinate lyase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111492127 PE=3 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 7.6e-259
Identity = 449/484 (92.77%), Postives = 470/484 (97.11%), Query Frame = 0

Query: 1   MTKLDVQSASQATGATSAASKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHD 60
           MT  DV SA++ATG TSAA+K+PEAKEIKLWGGRFQESVTDAVERFTESISYDKDL+KHD
Sbjct: 1   MTIFDVSSAAEATGETSAAAKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDLFKHD 60

Query: 61  IRGSRAHASMLAKQGLMSIGDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTD 120
           I GSRAHASMLAKQGLMSI DRD ILAGLDEIE+RI NGEFVWRADREDVHMNIEAALTD
Sbjct: 61  ISGSRAHASMLAKQGLMSISDRDCILAGLDEIEKRILNGEFVWRADREDVHMNIEAALTD 120

Query: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGY 180
           LIGEPAKKLHTARSRNDQVATDFRLWCRDAID I+ RIKDFQVA+VDLA+KN GIIVPGY
Sbjct: 121 LIGEPAKKLHTARSRNDQVATDFRLWCRDAIDLIIKRIKDFQVALVDLAIKNKGIIVPGY 180

Query: 181 THLQRAQPVLLQHVLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSS 240
           THLQRAQPVLLQH+LLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPI+RFMTSS
Sbjct: 181 THLQRAQPVLLQHLLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRFMTSS 240

Query: 241 ALEFSSPLRNSIDAVSDRDFAIEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVS 300
           AL FSSPLRNSIDAVSDRDFA+EFLSANSIMAIHLSRLGEEWVLWSSEEFGFI P+DAVS
Sbjct: 241 ALGFSSPLRNSIDAVSDRDFAMEFLSANSIMAIHLSRLGEEWVLWSSEEFGFIVPSDAVS 300

Query: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKT 360
           TGSSIMPQKKNPDPMELVRGKSARVIGD+VTLL+LCKGLPLAYNRDLQEDKEPVFDSVKT
Sbjct: 301 TGSSIMPQKKNPDPMELVRGKSARVIGDLVTLLSLCKGLPLAYNRDLQEDKEPVFDSVKT 360

Query: 361 VLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420
           V GMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM
Sbjct: 361 VSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAM 420

Query: 421 CLGKNCQLKDLSLNELRSLSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWIE 480
           CLGKNCQLKDLSL+ELR+LSPIF+EDVYEFLGVENAVNKFSSYGSTGSECVASQL++WIE
Sbjct: 421 CLGKNCQLKDLSLDELRALSPIFNEDVYEFLGVENAVNKFSSYGSTGSECVASQLEHWIE 480

Query: 481 KLGL 485
           KLG+
Sbjct: 481 KLGI 484

BLAST of CsaV3_2G030250 vs. TAIR 10
Match: AT5G10920.1 (L-Aspartase-like family protein )

HSP 1 Score: 749.2 bits (1933), Expect = 2.1e-216
Identity = 354/462 (76.62%), Postives = 416/462 (90.04%), Query Frame = 0

Query: 25  AKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHDIRGSRAHASMLAKQGLMSIGDRDS 84
           +KE+KLWGGRF+ESVT+ VE+FTESIS+DK LYK DI GS+AHASMLA QGL++  D+DS
Sbjct: 55  SKEVKLWGGRFEESVTEKVEKFTESISFDKVLYKQDIMGSKAHASMLAHQGLITDSDKDS 114

Query: 85  ILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFR 144
           IL GLD+IER+I   +F WR DREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFR
Sbjct: 115 ILRGLDDIERQIEANKFEWRTDREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFR 174

Query: 145 LWCRDAIDSIVARIKDFQVAMVDLAVKNAGIIVPGYTHLQRAQPVLLQHVLLAFVEQLDR 204
           LWCRDAID+I+ +I++ Q A+V+LA+KN  +IVPGYTHLQRAQPVLL HVLL FVEQL+R
Sbjct: 175 LWCRDAIDTIIVKIRNLQRALVELALKNEALIVPGYTHLQRAQPVLLPHVLLTFVEQLER 234

Query: 205 DAGRLLDCRARLNFCPLGACALAGTGLPIERFMTSSALEFSSPLRNSIDAVSDRDFAIEF 264
           DAGR +DCRARLNF PLGACALAGTGLPI+RFMT++AL F+ P+RNSIDAVSDRDF +EF
Sbjct: 235 DAGRYVDCRARLNFSPLGACALAGTGLPIDRFMTANALGFTEPMRNSIDAVSDRDFVLEF 294

Query: 265 LSANSIMAIHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGKSAR 324
           L  N+   IHLSRLGEEWVLW+SEEFGF+TP+D+VSTGSSIMPQKKNPDPMELVRGKSAR
Sbjct: 295 LYTNANTGIHLSRLGEEWVLWASEEFGFMTPSDSVSTGSSIMPQKKNPDPMELVRGKSAR 354

Query: 325 VIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKTVLGMLEVSAEFARNISFNQDRIKK 384
           VIGD+VT+LTLCKGLPLAYNRD QEDKEP+FDS KT++GM++VSAEFA+N++FN+DRIKK
Sbjct: 355 VIGDLVTVLTLCKGLPLAYNRDFQEDKEPMFDSTKTIMGMIDVSAEFAQNVTFNEDRIKK 414

Query: 385 ALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGKNCQLKDLSLNELRSLSPIFD 444
           +LPAGHLDATTLADYLV KG+PFR+SHDIVGK V +C+ K C+L++LSL E++ LSP+F+
Sbjct: 415 SLPAGHLDATTLADYLVKKGMPFRSSHDIVGKLVGVCVSKGCELQNLSLEEMKKLSPVFE 474

Query: 445 EDVYEFLGVENAVNKFSSYGSTGSECVASQLQYWIEKLGLIS 487
           EDV+ FLGVEN+VNKFSSYGSTGS CVA QL YW+ KL + S
Sbjct: 475 EDVFGFLGVENSVNKFSSYGSTGSNCVAEQLGYWVNKLNITS 516

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004152599.12.6e-277100.00argininosuccinate lyase, chloroplastic [Cucumis sativus] >KGN62752.1 hypothetica... [more]
KAA0065095.15.2e-27096.91argininosuccinate lyase [Cucumis melo var. makuwa][more]
XP_008444922.11.5e-26996.70PREDICTED: argininosuccinate lyase, chloroplastic-like [Cucumis melo][more]
XP_038886091.11.2e-26695.11argininosuccinate lyase, chloroplastic-like [Benincasa hispida][more]
XP_023546913.13.4e-26193.80argininosuccinate lyase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9LEU82.9e-21576.62Argininosuccinate lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g10... [more]
Q6AR604.9e-13051.80Argininosuccinate lyase OS=Desulfotalea psychrophila (strain LSv54 / DSM 12343) ... [more]
Q3JDS24.2e-12952.62Argininosuccinate lyase OS=Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 ... [more]
Q2SQ679.3e-12952.41Argininosuccinate lyase OS=Hahella chejuensis (strain KCTC 2396) OX=349521 GN=ar... [more]
Q5P7H31.6e-12851.74Argininosuccinate lyase OS=Aromatoleum aromaticum (strain EbN1) OX=76114 GN=argH... [more]
Match NameE-valueIdentityDescription
A0A0A0LPM31.3e-277100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G370510 PE=3 SV=1[more]
A0A5A7VGX52.5e-27096.91Argininosuccinate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A1S3BBH87.4e-27096.70argininosuccinate lyase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103488... [more]
A0A6J1HD891.4e-26093.39argininosuccinate lyase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1K8P17.6e-25992.77argininosuccinate lyase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT5G10920.12.1e-21676.62L-Aspartase-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00145ARGSUCLYASEcoord: 128..150
score: 72.51
coord: 220..236
score: 61.89
coord: 302..318
score: 81.13
coord: 258..282
score: 53.01
coord: 337..356
score: 76.96
coord: 169..189
score: 59.25
NoneNo IPR availableGENE3D1.20.200.10Fumarase/aspartase (Central domain)coord: 146..475
e-value: 9.2E-184
score: 613.2
NoneNo IPR availableGENE3D1.10.40.30coord: 387..459
e-value: 9.2E-184
score: 613.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR43814:SF4ARGININOSUCCINATE LYASEcoord: 39..484
IPR000362Fumarate lyase familyPRINTSPR00149FUMRATELYASEcoord: 258..285
score: 27.29
coord: 129..147
score: 38.14
coord: 174..192
score: 42.85
coord: 302..318
score: 64.09
IPR022761Fumarate lyase, N-terminalPFAMPF00206Lyase_1coord: 33..327
e-value: 2.0E-68
score: 231.1
IPR029419Argininosuccinate lyase, C-terminalPFAMPF14698ASL_C2coord: 392..458
e-value: 1.4E-21
score: 77.0
IPR024083Fumarase/histidase, N-terminalGENE3D1.10.275.10coord: 49..256
e-value: 9.2E-184
score: 613.2
IPR009049Argininosuccinate lyaseTIGRFAMTIGR00838TIGR00838coord: 30..477
e-value: 1.3E-171
score: 569.6
IPR009049Argininosuccinate lyasePANTHERPTHR43814ARGININOSUCCINATE LYASEcoord: 39..484
IPR009049Argininosuccinate lyaseHAMAPMF_00006Arg_succ_lyasecoord: 29..480
score: 39.630806
IPR009049Argininosuccinate lyaseCDDcd01359Argininosuccinate_lyasecoord: 49..482
e-value: 0.0
score: 651.919
IPR020557Fumarate lyase, conserved sitePROSITEPS00163FUMARATE_LYASEScoord: 302..311
IPR008948L-Aspartase-likeSUPERFAMILY48557L-aspartase-likecoord: 33..482

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_2G030250.1CsaV3_2G030250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042450 arginine biosynthetic process via ornithine
molecular_function GO:0004056 argininosuccinate lyase activity
molecular_function GO:0003824 catalytic activity