CsaV3_2G028050 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_2G028050
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	DPH4 homolog
Location	chr2: 18523895 .. 18526293 (+)
RNA-Seq Expression	CsaV3_2G028050
Synteny	CsaV3_2G028050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAATAACATTAATCATCAAATTTATACCCGAAATAAGCAAATATCTAATATATATATATATATATATATATATCTTATATTTAAAATAGGTATCAAATACCTTCTTCTACCTATCAAAGTCTAATTTGAATCGAAGGAGCCTAAAGTTATTCAATATAACCAAACCCATCCTTCATCAGCCCGCCTGCCCAATCTAACGGCCCAAACGCACTTGATCTAAAAGAGTAAATAAAAAGAAGAAAAAAATATATATAATCCCCCGCCTTCTGCGTCTCCATTTTCCGTTGCGTCCTACCATCCCGCCGCCGCGTTCTCCACCTTGCTCAGCACCGCAAACCTTCTCTGCCGATCTTCTTCCCTCCGTTGGTATAAGGTATTTTCTATATTACATATATATCTTTTGAGTTTCGATACTTACAATGGAAGAACGTCTGTAAGCTCAAACGAACGGGAAGATCGGTTCGATTTGAGAATCGATTTTTCCTAATCAAGGAGTTCAGATATTCCTTTGATTGTTCATTCTTAAGATCATTATTTTGATTATCAGTTTCGCCAAAAACCATAGAACCAAGAGACTTTTTATGTTATGGAAGATTTGAGTTGAACATTATGCTAGTTTAAAATTGAACAACTTTGGGTCTTTAGTGTTTCGTAGAGATTGAAATTGCTGATAGCCCAGTAGTCTAAGAAAAGTACGCTACCTAGGAGAACAAATTAAAAGGTAACTTTCCCACAACACCTTAATATCTTATACCTATGGAATTTTACTAACTATTTCATTTGCTCAACGAATTTGATATCATTTTTTTCATTAAGCTCGAAATTGGAACTTTCAACATCTATACTGTTTACCCCTTTTCAATAGGCTCTTGCACTGTAATGAGCTAATAATTTCCGTTGCATATATCTCAAGGTTGTTGGAAATTGAACCCATAATACAATGAATTCAATCAGTGGCTCCATTGGTGAAACTTACTATGATGTACTGTCTTTAAGAGAAGATGCTAGCTTTGACGAAATTCGAGCCAGCTATCGGTCGGCTCTCCTTAATTTCCACCCTGATAAATTACAAGCCATGTGCCATAAATCTCATCCAGACGACATCATGGGGGAAAGATACTTCAAGGTGCAGAAGGCTTGGGAAGTTCTCGGCAGCTCGAAATCCCGTGCATCTTATGACAGAGAGCTCCAAGCTGCCAAAGGGGATGCAATTGGTGCAGAGAGCATAAGCTTAGAGGATATGGTTGTGGAAGATAAAGGTGAAGTTGTAGAACTTCTTTATCAGTGCCGTTGTGGGGATTACTTCTTTATTGATTCCGGGGAGTTGGATGAAATGGGATATCCATTGTTGAGGAATGGTAGTAAGGTTTCTTTAAGGACTCTAAATGCTTTGCCTGCTTCTGTTGTTTTACCCTGTGGTTCTTGCTCTTTGAAGGTTCGTTTGCTAATTGATTCGAATTGACTGTTGAATTTGAATGATCGAAATCACAACGATTTCTCTTAATATCCTTGCCACTTGTTTAAATTGAGAGAGTTTATGAGAATTTTGTGCTCTTCGGTAGAGTTTACGTGGGTTTTCTTTAATGCAACTTTGTTGAGTGAAGTTCAACAATTTTCCTAGGCTGTTTGGTTCAACTTTGTACATGTGGATTGTGGGGGTTACTAATCGGCATCTGAAAGGCATCAGCTTGAAGACTTCGTCGTGTACTGAATTGTCAAAATTTGAGAGAACAACCAGAGTGTACACCAAGAGATGGGGGGAAATCCATGGTAGTTTCCTTCTCTAAAAATGACTGCTGGGTAAGATACATTGTTATTTCCTTCCTTCCCCTCGCAGGCTCTCTTGTAAAACCAAAGACGCCCGAAAACGAAGTACAACTGGAGGAGGAATTAATAAACAAGTCTGGCAAGGGGAATACTGACTGAACTATTTTAGAATAGGTTTAGTTAATTTTTGAGGTAGCGTATTTGCTAAATGATCAATTTGTTAATGACGACCTTTCTAGAAAACTTGTGGCAAACTGCCTAAGAAAATTCCCAAACTGGTGATTTGCTCCCTTGGATTGCTGCTTTTTTTTTTTTTCTGTAAAGGGGATGATTCCTTGATAATTTTCTACTTCACTGACCCTTTTGCCTTTGGTGGTAATCTTTCATGCCCCCCTAGATGGGTTAAGAATTGGCTAAAGGAGGCCTTAGGAGGTTGGTGTCTTGAAAGCGAGATGAAGATTCCCATCGGATGTAAGGGTAGTAAAATGATTATTTTTGTTGAAGTTTGCAGGATAACGTCTCGTGTTGGTGTTCTAGGCATACAAAGTTCATTAGTAATTGTAATATAATGACCAATCTTTAATCTTTCTTCTTATGATCTTTTACAATTGAAAAATCGTGGTTTTCTTC

mRNA sequence

ATGAATTCAATCAGTGGCTCCATTGGTGAAACTTACTATGATGTACTGTCTTTAAGAGAAGATGCTAGCTTTGACGAAATTCGAGCCAGCTATCGGTCGGCTCTCCTTAATTTCCACCCTGATAAATTACAAGCCATGTGCCATAAATCTCATCCAGACGACATCATGGGGGAAAGATACTTCAAGGTGCAGAAGGCTTGGGAAGTTCTCGGCAGCTCGAAATCCCGTGCATCTTATGACAGAGAGCTCCAAGCTGCCAAAGGGGATGCAATTGGTGCAGAGAGCATAAGCTTAGAGGATATGGTTGTGGAAGATAAAGGTGAAGTTGTAGAACTTCTTTATCAGTGCCGTTGTGGGGATTACTTCTTTATTGATTCCGGGGAGTTGGATGAAATGGGATATCCATTGTTGAGGAATGGTAGTAAGGTTTCTTTAAGGACTCTAAATGCTTTGCCTGCTTCTGTTGTTTTACCCTGTGGTTCTTGCTCTTTGAAGGTTCGTTTGCTAATTGATTCGAATTGA

Coding sequence (CDS)

ATGAATTCAATCAGTGGCTCCATTGGTGAAACTTACTATGATGTACTGTCTTTAAGAGAAGATGCTAGCTTTGACGAAATTCGAGCCAGCTATCGGTCGGCTCTCCTTAATTTCCACCCTGATAAATTACAAGCCATGTGCCATAAATCTCATCCAGACGACATCATGGGGGAAAGATACTTCAAGGTGCAGAAGGCTTGGGAAGTTCTCGGCAGCTCGAAATCCCGTGCATCTTATGACAGAGAGCTCCAAGCTGCCAAAGGGGATGCAATTGGTGCAGAGAGCATAAGCTTAGAGGATATGGTTGTGGAAGATAAAGGTGAAGTTGTAGAACTTCTTTATCAGTGCCGTTGTGGGGATTACTTCTTTATTGATTCCGGGGAGTTGGATGAAATGGGATATCCATTGTTGAGGAATGGTAGTAAGGTTTCTTTAAGGACTCTAAATGCTTTGCCTGCTTCTGTTGTTTTACCCTGTGGTTCTTGCTCTTTGAAGGTTCGTTTGCTAATTGATTCGAATTGA

Protein sequence

MNSISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERYFKVQKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGDYFFIDSGELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN*

Homology

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BLAST of CsaV3_2G028050 vs. NCBI nr
Match: XP_004152732.1 (DPH4 homolog [Cucumis sativus] >KGN62535.1 hypothetical protein Csa_022638 [Cucumis sativus])

HSP 1 Score: 347.8 bits (891), Expect = 5.3e-92
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0


Query: 1   MNSISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERY 60
           MNSISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERY
Sbjct: 1   MNSISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERY 60

Query: 61  FKVQKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGD 120
           FKVQKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGD
Sbjct: 61  FKVQKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN 174
           YFFIDSGELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN
Sbjct: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN 173

[Close]

BLAST of CsaV3_2G028050 vs. NCBI nr
Match: XP_038884890.1 (DPH4 homolog isoform X2 [Benincasa hispida])

HSP 1 Score: 329.3 bits (843), Expect = 2.0e-86
Identity = 164/173 (94.80%), Postives = 168/173 (97.11%), Query Frame = 0


Query: 1   MNSISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERY 60
           M+S SGSIGETYYDVLSL+EDAS+DEIRASYRSALLNFHPDKLQAMCHKSHPDDI  ERY
Sbjct: 1   MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERY 60

Query: 61  FKVQKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGD 120
           FKVQKAWEVLGSS SRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVEL YQCRCGD
Sbjct: 61  FKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN 174
           YFFIDSGELDEMGYPLLRNGSKVSLRTL+ALPASVVLPCGSCSLKVRLLIDSN
Sbjct: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSN 173

[Close]

BLAST of CsaV3_2G028050 vs. NCBI nr
Match: XP_008444636.1 (PREDICTED: DPH4 homolog [Cucumis melo])

HSP 1 Score: 323.2 bits (827), Expect = 1.4e-84
Identity = 161/173 (93.06%), Postives = 166/173 (95.95%), Query Frame = 0


Query: 1   MNSISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERY 60
           M+S SG+IGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMC KSHPDDI GERY
Sbjct: 1   MSSSSGAIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCRKSHPDDIKGERY 60

Query: 61  FKVQKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGD 120
           FKVQKAWEVLGS KSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVEL YQCRCGD
Sbjct: 61  FKVQKAWEVLGSPKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN 174
            FFIDSGELDEMGYPLLRNGSKVSLRTL+ALPASVVLPCGSCSLKV L+ID+N
Sbjct: 121 CFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVLLVIDTN 173

[Close]

BLAST of CsaV3_2G028050 vs. NCBI nr
Match: XP_022996470.1 (DPH4 homolog [Cucurbita maxima] >XP_022996471.1 DPH4 homolog [Cucurbita maxima])

HSP 1 Score: 317.8 bits (813), Expect = 5.9e-83
Identity = 158/173 (91.33%), Postives = 162/173 (93.64%), Query Frame = 0


Query: 1   MNSISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERY 60
           M+S SGSIGETYYDVLSLREDAS+DEIRASYRSALLNFHPDKLQAMCHKSH DDI GERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHLDDIAGERY 60

Query: 61  FKVQKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGD 120
            KVQKAWEVL SS SRA+YDRELQAAKGDAIGAESI LEDM VEDKGEVVEL YQCRCGD
Sbjct: 61  LKVQKAWEVLSSSMSRAAYDRELQAAKGDAIGAESIRLEDMAVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN 174
           YFFIDSGELDEMGYPLLRNG KVSLRTL+ LPASVVLPCGSCSLKVRLLIDSN
Sbjct: 121 YFFIDSGELDEMGYPLLRNGRKVSLRTLDVLPASVVLPCGSCSLKVRLLIDSN 173

[Close]

BLAST of CsaV3_2G028050 vs. NCBI nr
Match: XP_022962179.1 (DPH4 homolog [Cucurbita moschata] >XP_022962180.1 DPH4 homolog [Cucurbita moschata] >KAG7029347.1 JJJ3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 316.2 bits (809), Expect = 1.7e-82
Identity = 157/173 (90.75%), Postives = 162/173 (93.64%), Query Frame = 0


Query: 1   MNSISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERY 60
           M+S SGSIGETYYDVLSLREDAS+DEIRASYRSALLNFHPDKLQAMCHKSH DDI GERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHLDDIAGERY 60

Query: 61  FKVQKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGD 120
            KVQKAWEVL SS SRA+YDRELQAAKGDAIGAESI LEDM VEDKGEVVEL YQCRCGD
Sbjct: 61  LKVQKAWEVLSSSMSRAAYDRELQAAKGDAIGAESIRLEDMAVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN 174
           YFFIDSGELDEMGYPLLRNG KVSLRTL+ LPASVVLPCGSCSL+VRLLIDSN
Sbjct: 121 YFFIDSGELDEMGYPLLRNGRKVSLRTLDVLPASVVLPCGSCSLEVRLLIDSN 173

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BLAST of CsaV3_2G028050 vs. ExPASy Swiss-Prot
Match: Q54CI5 (DPH4 homolog OS=Dictyostelium discoideum OX=44689 GN=dph4 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.8e-10
Identity = 45/156 (28.85%), Postives = 74/156 (47.44%), Query Frame = 0


Query: 10  ETYYDVLSLREDASFDEIRASYRSALLNFHPDKL--------------QAMCHKSHPDDI 69
           + YY++L +  DA  +EI+ SYR   L +HPDKL                + + ++ ++ 
Sbjct: 14  KNYYEILKVSIDADIEEIKKSYRKLALLYHPDKLNKEENIEENINNFSNCLVNNNNNNNN 73

Query: 70  MGERYFK-VQKAWEVLGSSKSRASYDRELQAAKGDAIG-AESISLEDM-VVEDKGEVVEL 129
              + F  +Q AWE L     R  YD  L   K      ++ I L+DM  +E+  E V  
Sbjct: 74  SNTKDFNDIQIAWETLKDDLLRKQYDSLLLEQKRQKYSVSDEIDLDDMEFIEENSEYV-- 133

Query: 130 LYQCRCGDYFFIDSGELDEMGYPLLRNGSKVSLRTL 149
            Y CRCGD++ I   +L E    +  +G  +S++ +
Sbjct: 134 -YPCRCGDHYIITEDQLSEGSDVVCCSGCSLSIKVI 166

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BLAST of CsaV3_2G028050 vs. ExPASy Swiss-Prot
Match: P47138 (Diphthamide biosynthesis protein 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=JJJ3 PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.8e-10
Identity = 49/156 (31.41%), Postives = 75/156 (48.08%), Query Frame = 0


Query: 11  TYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERYFKVQKAWEVL 70
           T+Y++L +  DA+ DEI+ +YR+ LLN HPDKL    H    D +      K+Q A+++L
Sbjct: 8   THYEILRIPSDATQDEIKKAYRNRLLNTHPDKLSKSIH----DTVSNVTINKIQDAYKIL 67

Query: 71  GSSKSRASYDR------ELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQC-RC---GD 130
            + K+R  YDR      + Q       G +  SL+D   ++  + +E +  C RC   G 
Sbjct: 68  SNIKTRREYDRLILENYKRQGFHNCGDGLDEFSLDDFSFDE--DKLEFMMNCPRCQFVGG 127

Query: 131 YFF-----------IDSGELDEMGYPLLRNGSKVSL 146
           + F           +D+ E    GY LL   S  SL
Sbjct: 128 FHFSESLLDECIDNVDAMERSHSGYQLLTQCSACSL 157

[Close]

BLAST of CsaV3_2G028050 vs. ExPASy Swiss-Prot
Match: Q6FWM1 (Diphthamide biosynthesis protein 4 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=DPH4 PE=3 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.1e-07
Identity = 41/130 (31.54%), Postives = 67/130 (51.54%), Query Frame = 0


Query: 11  TYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERYFKVQKAWEVL 70
           ++Y+VL +  DAS DEI+ +YR  LL  HPDK + +   S      G    ++Q+A+ VL
Sbjct: 10  SHYEVLGVPVDASTDEIKLTYRKMLLTLHPDKSKTVPIDSTIKIRSGVSINQIQEAYRVL 69

Query: 71  GSSKSRASYDRELQAAK--------GDAIGAESISLEDMVV-EDKGEVVELLYQCRCGDY 130
            + + RA+Y+R L  +         GD  G +  +L++    E+K E V    +C+ G  
Sbjct: 70  SNDQLRAAYNRALNDSNKLAGFNNFGD--GLDEFNLDEFEFNEEKLEYVMTCPRCQSGGG 129

Query: 131 FFIDSGELDE 132
           F +    L+E
Sbjct: 130 FVLSEDMLEE 137

[Close]

BLAST of CsaV3_2G028050 vs. ExPASy Swiss-Prot
Match: B9KH92 (Chaperone protein DnaJ OS=Anaplasma marginale (strain Florida) OX=320483 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.5e-07
Identity = 29/74 (39.19%), Postives = 44/74 (59.46%), Query Frame = 0


Query: 9  GETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERYF-KVQKAW 68
          G  YY++L +  +AS +EI+ SYR  +  +HPDK        +P D   E  F K+ +A+
Sbjct: 3  GNDYYEILEVSRNASAEEIKKSYRKMVFKYHPDK--------NPGDKKAEEKFKKISEAY 62

Query: 69 EVLGSSKSRASYDR 82
          EVL + + RA+YDR
Sbjct: 63 EVLSNPEKRAAYDR 68

[Close]

BLAST of CsaV3_2G028050 vs. ExPASy Swiss-Prot
Match: Q5P9E0 (Chaperone protein DnaJ OS=Anaplasma marginale (strain St. Maries) OX=234826 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.5e-07
Identity = 29/74 (39.19%), Postives = 44/74 (59.46%), Query Frame = 0


Query: 9  GETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERYF-KVQKAW 68
          G  YY++L +  +AS +EI+ SYR  +  +HPDK        +P D   E  F K+ +A+
Sbjct: 3  GNDYYEILEVSRNASAEEIKKSYRKMVFKYHPDK--------NPGDKKAEEKFKKISEAY 62

Query: 69 EVLGSSKSRASYDR 82
          EVL + + RA+YDR
Sbjct: 63 EVLSNPEKRAAYDR 68

[Close]

BLAST of CsaV3_2G028050 vs. ExPASy TrEMBL
Match: A0A0A0LKX8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G360030 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 2.6e-92
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0


Query: 1   MNSISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERY 60
           MNSISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERY
Sbjct: 1   MNSISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERY 60

Query: 61  FKVQKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGD 120
           FKVQKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGD
Sbjct: 61  FKVQKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN 174
           YFFIDSGELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN
Sbjct: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN 173

[Close]

BLAST of CsaV3_2G028050 vs. ExPASy TrEMBL
Match: A0A1S3BBM0 (DPH4 homolog OS=Cucumis melo OX=3656 GN=LOC103487907 PE=3 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 6.8e-85
Identity = 161/173 (93.06%), Postives = 166/173 (95.95%), Query Frame = 0


Query: 1   MNSISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERY 60
           M+S SG+IGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMC KSHPDDI GERY
Sbjct: 1   MSSSSGAIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCRKSHPDDIKGERY 60

Query: 61  FKVQKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGD 120
           FKVQKAWEVLGS KSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVEL YQCRCGD
Sbjct: 61  FKVQKAWEVLGSPKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN 174
            FFIDSGELDEMGYPLLRNGSKVSLRTL+ALPASVVLPCGSCSLKV L+ID+N
Sbjct: 121 CFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVLLVIDTN 173

[Close]

BLAST of CsaV3_2G028050 vs. ExPASy TrEMBL
Match: A0A6J1K4U3 (DPH4 homolog OS=Cucurbita maxima OX=3661 GN=LOC111491712 PE=3 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 2.9e-83
Identity = 158/173 (91.33%), Postives = 162/173 (93.64%), Query Frame = 0


Query: 1   MNSISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERY 60
           M+S SGSIGETYYDVLSLREDAS+DEIRASYRSALLNFHPDKLQAMCHKSH DDI GERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHLDDIAGERY 60

Query: 61  FKVQKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGD 120
            KVQKAWEVL SS SRA+YDRELQAAKGDAIGAESI LEDM VEDKGEVVEL YQCRCGD
Sbjct: 61  LKVQKAWEVLSSSMSRAAYDRELQAAKGDAIGAESIRLEDMAVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN 174
           YFFIDSGELDEMGYPLLRNG KVSLRTL+ LPASVVLPCGSCSLKVRLLIDSN
Sbjct: 121 YFFIDSGELDEMGYPLLRNGRKVSLRTLDVLPASVVLPCGSCSLKVRLLIDSN 173

[Close]

BLAST of CsaV3_2G028050 vs. ExPASy TrEMBL
Match: A0A6J1HGB5 (DPH4 homolog OS=Cucurbita moschata OX=3662 GN=LOC111462713 PE=3 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 8.3e-83
Identity = 157/173 (90.75%), Postives = 162/173 (93.64%), Query Frame = 0


Query: 1   MNSISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERY 60
           M+S SGSIGETYYDVLSLREDAS+DEIRASYRSALLNFHPDKLQAMCHKSH DDI GERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHLDDIAGERY 60

Query: 61  FKVQKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGD 120
            KVQKAWEVL SS SRA+YDRELQAAKGDAIGAESI LEDM VEDKGEVVEL YQCRCGD
Sbjct: 61  LKVQKAWEVLSSSMSRAAYDRELQAAKGDAIGAESIRLEDMAVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN 174
           YFFIDSGELDEMGYPLLRNG KVSLRTL+ LPASVVLPCGSCSL+VRLLIDSN
Sbjct: 121 YFFIDSGELDEMGYPLLRNGRKVSLRTLDVLPASVVLPCGSCSLEVRLLIDSN 173

[Close]

BLAST of CsaV3_2G028050 vs. ExPASy TrEMBL
Match: A0A6J1CSL8 (DPH4 homolog OS=Momordica charantia OX=3673 GN=LOC111013930 PE=3 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 1.1e-82
Identity = 156/173 (90.17%), Postives = 164/173 (94.80%), Query Frame = 0


Query: 1   MNSISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERY 60
           M+S  G IGETYYDVLSL+ DAS+DEIRASYRSALLNFHPDKLQAMC KSHPDD+ GERY
Sbjct: 1   MSSGYGFIGETYYDVLSLKHDASYDEIRASYRSALLNFHPDKLQAMCQKSHPDDLSGERY 60

Query: 61  FKVQKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGD 120
           FKVQKAWEVLGSS SRA+YDRELQAAKGDA+GAESISLEDMVVEDKGEVVEL YQCRCGD
Sbjct: 61  FKVQKAWEVLGSSTSRAAYDRELQAAKGDAMGAESISLEDMVVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN 174
           YFFIDSGEL+EMGYPLLRNGSKVSLRTL ALPASVVLPCGSCSLKVRLLIDS+
Sbjct: 121 YFFIDSGELEEMGYPLLRNGSKVSLRTLEALPASVVLPCGSCSLKVRLLIDSS 173

[Close]

BLAST of CsaV3_2G028050 vs. TAIR 10
Match: AT4G10130.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 187.2 bits (474), Expect = 1.1e-47
Identity = 90/166 (54.22%), Postives = 122/166 (73.49%), Query Frame = 0


Query: 8   IGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERYFKVQKAW 67
           + ETYY++LS++EDAS++EIR SYRSA+L+ HPDKL      S  D    E++ K+QKAW
Sbjct: 8   VHETYYEILSVKEDASYEEIRNSYRSAILHSHPDKLNNTSRSSSDD----EKFLKIQKAW 67

Query: 68  EVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGDYFFIDSG 127
           EVL  ++ R  YD +L++++ D I A+ IS+EDM VE  G+V++L YQCRCGDYF +DS 
Sbjct: 68  EVLSDAELRVVYDNDLRSSRHDGITADEISIEDMSVEITGDVIDLFYQCRCGDYFCVDSS 127

Query: 128 ELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN 174
           EL  MG+ LLR+G  V ++ L A  ASVVLPCGSCSLK R+ +DS+
Sbjct: 128 ELGTMGFALLRDGDFVYVKRLGAFVASVVLPCGSCSLKTRVWVDSD 169

[Close]

BLAST of CsaV3_2G028050 vs. TAIR 10
Match: AT4G37480.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 50.8 bits (120), Expect = 1.3e-06
Identity = 27/74 (36.49%), Postives = 41/74 (55.41%), Query Frame = 0


Query: 10  ETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERYFKVQKAWEV 69
           E  YD+L++ E +S  EI+AS+R      HPD ++     S  D     R+ ++  A+E+
Sbjct: 55  ENAYDILNVSETSSIAEIKASFRRLAKETHPDLIE-----SKKDPSNSRRFVQILAAYEI 114

Query: 70  LGSSKSRASYDREL 84
           L  S+ RA YDR L
Sbjct: 115 LSDSEKRAHYDRYL 123

[Close]

BLAST of CsaV3_2G028050 vs. TAIR 10
Match: AT1G56300.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 49.7 bits (117), Expect = 2.8e-06
Identity = 32/113 (28.32%), Postives = 57/113 (50.44%), Query Frame = 0


Query: 7   SIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGE---RYFKV 66
           ++  +YY +L +R+DAS  +IR +YR   + +HPD+       +    + GE   R+ ++
Sbjct: 9   NVRSSYYTILGIRKDASVSDIRTAYRKLAMKWHPDRY------ARNPGVAGEAKRRFQQI 68

Query: 67  QKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMV-----VEDKGEVVE 112
           Q+A+ VL     R+ YD  L     D        +++M+     V+D GE +E
Sbjct: 69  QEAYSVLNDENKRSMYDVGLYDPHEDDDDDFCDFMQEMISMMNNVKDAGESLE 115

[Close]

BLAST of CsaV3_2G028050 vs. TAIR 10
Match: AT1G74250.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 49.7 bits (117), Expect = 2.8e-06
Identity = 31/80 (38.75%), Postives = 44/80 (55.00%), Query Frame = 0


Query: 1  MNSISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERY 60
          M S S S    +Y+VL + +++S DEIR+SYR   L  HPDKL      S  +     ++
Sbjct: 1  MASSSRSEKRCHYEVLGISKESSPDEIRSSYRRLALQRHPDKLMKAAGLSEAE--ATAQF 60

Query: 61 FKVQKAWEVLGSSKSRASYD 81
           ++  A+EVL   K RA YD
Sbjct: 61 QELVHAYEVLSDPKERAWYD 78

[Close]

BLAST of CsaV3_2G028050 vs. TAIR 10
Match: AT1G71000.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 1.4e-05
Identity = 24/77 (31.17%), Postives = 43/77 (55.84%), Query Frame = 0


Query: 4  ISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERYFKV 63
          + G I +TYY++L +  D+S ++IR +Y      +HPD+      +S        R+ ++
Sbjct: 1  MEGGIRQTYYEILGVAVDSSAEQIRRAYHKLAKIWHPDRWTKDPFRSGE---AKRRFQQI 60

Query: 64 QKAWEVLGSSKSRASYD 81
          Q+A+ VL   + R+SYD
Sbjct: 61 QEAYSVLSDERKRSSYD 74

The following BLAST results are available for this feature:

BLAST of CsaV3_2G028050 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152732.1	5.3e-92	100.00	DPH4 homolog [Cucumis sativus] >KGN62535.1 hypothetical protein Csa_022638 [Cucu...	[more]
XP_038884890.1	2.0e-86	94.80	DPH4 homolog isoform X2 [Benincasa hispida]	[more]
XP_008444636.1	1.4e-84	93.06	PREDICTED: DPH4 homolog [Cucumis melo]	[more]
XP_022996470.1	5.9e-83	91.33	DPH4 homolog [Cucurbita maxima] >XP_022996471.1 DPH4 homolog [Cucurbita maxima]	[more]
XP_022962179.1	1.7e-82	90.75	DPH4 homolog [Cucurbita moschata] >XP_022962180.1 DPH4 homolog [Cucurbita moscha...	[more]

BLAST of CsaV3_2G028050 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54CI5	1.8e-10	28.85	DPH4 homolog OS=Dictyostelium discoideum OX=44689 GN=dph4 PE=3 SV=1	[more]
P47138	1.8e-10	31.41	Diphthamide biosynthesis protein 4 OS=Saccharomyces cerevisiae (strain ATCC 2045...	[more]
Q6FWM1	1.1e-07	31.54	Diphthamide biosynthesis protein 4 OS=Candida glabrata (strain ATCC 2001 / CBS 1...	[more]
B9KH92	2.5e-07	39.19	Chaperone protein DnaJ OS=Anaplasma marginale (strain Florida) OX=320483 GN=dnaJ...	[more]
Q5P9E0	2.5e-07	39.19	Chaperone protein DnaJ OS=Anaplasma marginale (strain St. Maries) OX=234826 GN=d...	[more]

BLAST of CsaV3_2G028050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LKX8	2.6e-92	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G360030 PE=3 SV=1	[more]
A0A1S3BBM0	6.8e-85	93.06	DPH4 homolog OS=Cucumis melo OX=3656 GN=LOC103487907 PE=3 SV=1	[more]
A0A6J1K4U3	2.9e-83	91.33	DPH4 homolog OS=Cucurbita maxima OX=3661 GN=LOC111491712 PE=3 SV=1	[more]
A0A6J1HGB5	8.3e-83	90.75	DPH4 homolog OS=Cucurbita moschata OX=3662 GN=LOC111462713 PE=3 SV=1	[more]
A0A6J1CSL8	1.1e-82	90.17	DPH4 homolog OS=Momordica charantia OX=3673 GN=LOC111013930 PE=3 SV=1	[more]

BLAST of CsaV3_2G028050 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10130.1	1.1e-47	54.22	DNAJ heat shock N-terminal domain-containing protein	[more]
AT4G37480.1	1.3e-06	36.49	Chaperone DnaJ-domain superfamily protein	[more]
AT1G56300.1	2.8e-06	28.32	Chaperone DnaJ-domain superfamily protein	[more]
AT1G74250.1	2.8e-06	38.75	DNAJ heat shock N-terminal domain-containing protein	[more]
AT1G71000.1	1.4e-05	31.17	Chaperone DnaJ-domain superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 75..94 score: 24.62 coord: 31..46 score: 34.71 coord: 55..75 score: 22.64 coord: 13..31 score: 38.0
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 10..75 e-value: 1.3E-9 score: 48.0
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 11..80 e-value: 1.6E-14 score: 53.8
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 11..83 score: 14.667232
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 11..71 e-value: 3.22318E-13 score: 59.0961
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 5..86 e-value: 5.1E-17 score: 63.9
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 10..83
IPR036671	DPH-type metal-binding domain superfamily	GENE3D	3.10.660.10	DPH Zinc finger	coord: 87..173 e-value: 1.3E-15 score: 58.6
IPR036671	DPH-type metal-binding domain superfamily	SUPERFAMILY	144217	CSL zinc finger	coord: 92..171
IPR007872	DPH-type metal-binding domain	PFAM	PF05207	zf-CSL	coord: 95..169 e-value: 1.2E-12 score: 47.3
IPR007872	DPH-type metal-binding domain	PROSITE	PS51074	DPH_MB	coord: 92..171 score: 14.693018
None	No IPR available	PANTHER	PTHR21454:SF31	DIPHTHAMIDE BIOSYNTHESIS PROTEIN 4	coord: 3..171
IPR044248	Diphthamide biosynthesis protein 3/4-like	PANTHER	PTHR21454	DPH3 HOMOLOG-RELATED	coord: 3..171

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_2G028050.1	CsaV3_2G028050.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017183	peptidyl-diphthamide biosynthetic process from peptidyl-histidine
molecular_function	GO:0046872	metal ion binding