CsaV3_2G027840 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_2G027840
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	Calcium-binding protein CML16
Location	chr2: 18415744 .. 18419969 (+)
RNA-Seq Expression	CsaV3_2G027840
Synteny	CsaV3_2G027840

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGAACTCCAAAAGCAAAACGTACAAACGAGTTCTAGTTCCATTCTCAGAGGAGCAACTGGCCAACGTTTTCAAGAACCATGACCGAGACGGGGATGGGCATCTGACGAAGGAGGAGTTGAAGCAAGCCTTCGAATATCTCGGCTCACGCTTCTGCAACTTCCGGGTAGAAGAAGCCCTACGTGCCGCCGATACCAACGGGGACGGTGTCATCAGCATGGATGAGATGGGGAAGCTAATTAAGTATGCCAAAACTCGTAAATACACTATTAGTTAAAAAGTAAATGTATGTTAGCTATATCAACTTCAGTTGTTCTTGTTCACCTATATATATATATATATATTATATATATGTCTTCATATATTTATCTGTATTTGAATGAGTGGATTCGAATTAATACCCCTCTTTGTCCAACGATATATAAATAATAATAAAAAAAATTGTTAAATGGTTTTGTTGATTGTGTATTGATTCGATCTCACTCTCTGCTTGTCTCTAATATAAAGAATGGACCTAATTTAATTTGCAGGGAGATGGAACAATAATATTATGATATGATTTGAACTCATGATTCGATGTACGTCGTGTTTGGAATCATTTTTAATTTTTTTTTCTCTGGAAATATTAATAATTTAGAGTGTATTTAACATAGTCTTCAATTATTTGAGATGATACTAAAATTTTCTTTAAAAAATTCTTAATAAATGAAAAGTAGATTAAAGAATTTGGATTCATTTAAAATCATCTTTCAATGATCTAGCTGATGCCTTATCTATAATATAGTTTTTTTTTTTTTAACTCAATTACCATTTTTTTCTTAAAATTAATTTTGTTAAAATATATCACAGTTCAAGTATTGGCAACCTACGATATTAATCCATTCTATTCTCTCTCATGTATAATAACAAGATTGCATCTTGTATATATACAAACACTTCTATAAATTTTCAAGGTCTTTTTAGCACAATAAAGCTAGGAGAAATTTTGGTCTTCACATGCATCATTTTAAACTTAAAAATAAAATAATATTACCAATAGTCTAAAAATTCTTCCATAGATTCAATTCTTTTACTAAATAGAGTTTTCGGTCTCATATTTTGTCACCAAGTTTTAGACTAGAATGTTTCCTTTAATAGAACATTTAGAGTTAAATGACTTTCCACTTCTAGTTAAGATAGAAGATCACACCAATTATTACAAATGTACTAAACTTGTTTGGACTAATCGTTTTTTTCGAATAAAACGCAAAACCATAGTCATGGATTTGTTTATACCCTAACATGAAAGAATATTGATTGTTTATTTATTTTTAATTCTTTTTTGTTAAGCTTCTCATTAGGTGTTTTTAATATTACAATAATAAAAATAAAAATAAAAATAAAGGGAAGTTGGTCAAATTTTGGAATGAAATTCGTGAAAATCTGATTAGTACGGTCTCCCTATCTTAGGGGCAATTCCAATTTCTAAATACACCAAATAAAGTATTAACATATATATAATACTCCAAAATCATTTATAACTATAACAAAAAATTTAAATACAAACCTATCACTATTTAGAATTTAACGACCTATAATAAAATTTATGAATAACAAATTTTACTATATTTTAACACATATTCAAAAAAATTTCTTCATCTTGTAACTTATATCTATATATATATTAATAATTTTAATCGTCATCACCAAATATATTTATATGTGATCGACTTGAATATAGTTCAACTCATTAAAACATTATACTTATATTTTTGCTTGAGATGTCAAATATTCTATTTTCTCTCTTTACAACTGTGTCCATGTAAGCAATGGAATCCAATTACATCTAATACCCATCTGTATTTACTTCTCAATTTAGAATTACTTTCCTTTCCCATTCAATATACCTAATAAATTACTTATTGATATTAGAATGCAAACTATACCCATATACCCCAATTGTGAGCATCTCAACAAGAAAAATTCTTTCAACGATGAATTGTTTTTTTAATTAAATATTTTGAAGATACAGTGGTATTTTATAAAAAGAAACAAAATTTTGAGCATTTTTTATTGGTAAGATTAGGTAGATATATATAAAGATATTTATTTATCAATTCCTATTAATCAATTTTAATTAATAATCAGAAAGTTGGTAACAACTTTTTCTGTGGGTTTCTCTCGTAATGTTTTAGAGATAGATAGGGTTTAGAGAAAGAATATGGAGATTTGTATTAGAGTTTACAATCATGATTACAACAAAGATATGACTAAGCTTAAATATTAAAGTCTAAGGTCCAATGGACTTGTGTACATGTAAAATAATAAACTAATACTGATTGTTAATTAACATCAATTAGCATATATAATATAAACCTTATTAATACACATCAAAACTACATACTTTTAACCCTTTCAAGTATGCATTACTGTGATTCTTTTTGTAACTTAATTTAGCCTCCACTTTAATATTATTTTGTTTCTATAAACTAAATTTATAAACACTACTTGTCCATAGGTTTTTATATTTTCTAATTATCTGTCTTCTATAAACAAATACAAAAACCAAGTTTTAGAAACTAATTGAAAAACTAGTTTTTTTTAAAAAATATTTATTTTCGTTTTTAGAATTGAACATTGAAAATTTACCATAAAAAAAGAGAGAAAACGAACTCATTTTTCTTCTTTTTTTTTTTCTTTTTAAGATAAAATAAAATATTTATAAAATGAAGCACATTACTTTTGTTGTTGTGTGTTCTTTTAATGTCATTTCTTGAATATATTTTAGAATTTTGATTGATTTTGAGTTAATCTTGACCATCTATCTCACACGAGAACAATAGAAAATATAAATCTACTGTACATATCAATTTGTAAATACAAGCTATTAAGGTTAAACATGTTAACCACAGACGATTATTTTTATGTATCTCTATAATACTATGATACATATTTTTGAGTTTACACCTTTGTTTTTTATTTCATAAAAAAATTATACCAATAAATTAAAAATAATTGCCCCATTTCAATTTTCGAAGACATTGTATTTTCTCTACTGCGCCATTGACACTACCCAGACCATTTGTATTATGTTTGGTGTGTACTCAACCTAACAACTTTCCCTATTAAGTCACACCCTACTTTTAAAAAGAATATGCTTTCCATCAAATGTTGAAATTAATCTTCGTATAAAATGGATCTTTGGGAATTAAATTTGTTAGCTACCAACTTTCTGATACAGAGAAAAATAAAACTTATTATTTACAAAAGTAATCTCTCGTAATGATCTAGGGCATGAAAACTTGAGTATTATGTATTTTCAAGAAAGACAAATGATAAAAACTACATTGATGATATAGTTGTAAATGTATACCTTCAAATTTTTAATTGGAAAATCAAAGGTTAAAATTGAACCCTTAAACTAATTACATAATTGTAGGAATGGTAACAATTGTTTGCTATGTCTAAAGAATCAATTTTTATCATTCAAGGTCCTACTCTTAATAGAAGAAACAAAACACCTTTGAAGATGATGAGTCCACCTGTGCATTATTTTACATGTTTATAGATAGTTCTTAATATAAAAAGAAAAGTTGAAATGTAAGAACTAAATTTATATATATAAAGAAAAAAAAAAACACATGTTTCTAAAAAGTTTTAAATTAATAGAAATTAGGTGGCCTAAAAATTCCACTCCTTTTATCTTCCTTCATAGGTCAAGAAAAAGGGAAACCAACAATTCTCCCTCTCTCCTTCGTAACAGAAGAAACACATGTTCAAAAAAATTGAGATTTGGTAGGCTGCTTGCTTCATGAAGATGGTGAGTCCAAACGTGTGAGAAAGAAACACCGCACGTTCATTTTACATGTTTATAGATATAAATCTCTCCCATTGTAAATTAAACCAACACAAAAGTCTTTCCCTCCTCTTACCTGTTCTTCTTTTAGAGCAAAACCCTTATACACGTATTATAACCCATCCATGGGGACCTCCAACGGCTTGATTTTTAAGTGTTTTCCAGCGTCGTTGACGGAGTTTGAGGTGGCCAAAGTTTTCAAGAACCACGACAAGGATGGAGATGGAAGGTTGACCAAGGAAGAGCTGAAGAGCGCCTTCAAGTATTTGGGCTACAAGTTCACCACCTTTAGAGTCCACCAAGCCCTCCACGCCGCTGACGACAACTGCGACGATGTCATCAGCTTTGAGGAGATGAGTAAACTTATTATGTATACCGCAGGTTCATAG

mRNA sequence

ATGGGGAACTCCAAAAGCAAAACGTACAAACGAGTTCTAGTTCCATTCTCAGAGGAGCAACTGGCCAACGTTTTCAAGAACCATGACCGAGACGGGGATGGGCATCTGACGAAGGAGGAGTTGAAGCAAGCCTTCGAATATCTCGGCTCACGCTTCTGCAACTTCCGGGTAGAAGAAGCCCTACGTGCCGCCGATACCAACGGGGACGGTGTCATCAGCATGGATGAGATGGGGAAGCTAATTAAAGCAAAACCCTTATACACGTATTATAACCCATCCATGGGGACCTCCAACGGCTTGATTTTTAAGTGTTTTCCAGCGTCGTTGACGGAGTTTGAGGTGGCCAAAGTTTTCAAGAACCACGACAAGGATGGAGATGGAAGGTTGACCAAGGAAGAGCTGAAGAGCGCCTTCAAGTATTTGGGCTACAAGTTCACCACCTTTAGAGTCCACCAAGCCCTCCACGCCGCTGACGACAACTGCGACGATGTCATCAGCTTTGAGGAGATGAGTAAACTTATTATGTATACCGCAGGTTCATAG

Coding sequence (CDS)

ATGGGGAACTCCAAAAGCAAAACGTACAAACGAGTTCTAGTTCCATTCTCAGAGGAGCAACTGGCCAACGTTTTCAAGAACCATGACCGAGACGGGGATGGGCATCTGACGAAGGAGGAGTTGAAGCAAGCCTTCGAATATCTCGGCTCACGCTTCTGCAACTTCCGGGTAGAAGAAGCCCTACGTGCCGCCGATACCAACGGGGACGGTGTCATCAGCATGGATGAGATGGGGAAGCTAATTAAAGCAAAACCCTTATACACGTATTATAACCCATCCATGGGGACCTCCAACGGCTTGATTTTTAAGTGTTTTCCAGCGTCGTTGACGGAGTTTGAGGTGGCCAAAGTTTTCAAGAACCACGACAAGGATGGAGATGGAAGGTTGACCAAGGAAGAGCTGAAGAGCGCCTTCAAGTATTTGGGCTACAAGTTCACCACCTTTAGAGTCCACCAAGCCCTCCACGCCGCTGACGACAACTGCGACGATGTCATCAGCTTTGAGGAGATGAGTAAACTTATTATGTATACCGCAGGTTCATAG

Protein sequence

MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEALRAADTNGDGVISMDEMGKLIKAKPLYTYYNPSMGTSNGLIFKCFPASLTEFEVAKVFKNHDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLIMYTAGS*

Homology

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BLAST of CsaV3_2G027840 vs. NCBI nr
Match: KGN62765.2 (hypothetical protein Csa_022396 [Cucumis sativus])

HSP 1 Score: 372.9 bits (956), Expect = 1.6e-99
Identity = 180/180 (100.00%), Postives = 180/180 (100.00%), Query Frame = 0


Query: 1   MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEA 60
           MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEA
Sbjct: 1   MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEA 60

Query: 61  LRAADTNGDGVISMDEMGKLIKAKPLYTYYNPSMGTSNGLIFKCFPASLTEFEVAKVFKN 120
           LRAADTNGDGVISMDEMGKLIKAKPLYTYYNPSMGTSNGLIFKCFPASLTEFEVAKVFKN
Sbjct: 61  LRAADTNGDGVISMDEMGKLIKAKPLYTYYNPSMGTSNGLIFKCFPASLTEFEVAKVFKN 120

Query: 121 HDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLIMYTAGS 180
           HDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLIMYTAGS
Sbjct: 121 HDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLIMYTAGS 180

[Close]

BLAST of CsaV3_2G027840 vs. NCBI nr
Match: KAG7020566.1 (hypothetical protein SDJN02_17252, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 214.9 bits (546), Expect = 5.6e-52
Identity = 113/177 (63.84%), Postives = 133/177 (75.14%), Query Frame = 0


Query: 1   MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEA 60
           MGN+KS  YKRVLVPF+EEQLA +FK+HDRDGDG L+KEELKQAF YLGSRF +FRV+EA
Sbjct: 36  MGNAKSTNYKRVLVPFTEEQLAAIFKSHDRDGDGKLSKEELKQAFNYLGSRFSSFRVKEA 95

Query: 61  LRAADTNGDGVISMDEMGKLIKAKPLYTYYNPSMGTSNGLIFKCFPASLTEFEVAKVFKN 120
           LRAADTNGDG ISMDEM KLIK    YT          GL+       LTE ++A +FK+
Sbjct: 96  LRAADTNGDGFISMDEMNKLIK----YT---------KGLL-----VPLTEEQLAGIFKS 155

Query: 121 HDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLIMYT 178
           HD+DGDG+LTKEELK AF YLG +F++FR  +AL AAD + D  I+  EMSKLI YT
Sbjct: 156 HDRDGDGKLTKEELKQAFNYLGSRFSSFRADEALRAADADGDGTINMAEMSKLIQYT 194

[Close]

BLAST of CsaV3_2G027840 vs. NCBI nr
Match: KAA0060899.1 (putative Calcium-binding EF-hand family protein [Cucumis melo var. makuwa])

HSP 1 Score: 162.5 bits (410), Expect = 3.3e-36
Identity = 80/82 (97.56%), Postives = 81/82 (98.78%), Query Frame = 0


Query: 1  MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEA 60
          MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDG+LTKEELKQAFEYLGSRF NFRVEEA
Sbjct: 1  MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGNLTKEELKQAFEYLGSRFSNFRVEEA 60

Query: 61 LRAADTNGDGVISMDEMGKLIK 83
          LRAADTNGDGVISMDEMGKLIK
Sbjct: 61 LRAADTNGDGVISMDEMGKLIK 82

[Close]

BLAST of CsaV3_2G027840 vs. NCBI nr
Match: KAA0060898.1 (putative Calcium-binding EF-hand family protein [Cucumis melo var. makuwa])

HSP 1 Score: 152.5 bits (384), Expect = 3.4e-33
Identity = 74/87 (85.06%), Postives = 79/87 (90.80%), Query Frame = 0


Query: 94  MGTSNGLIFKCFPASLTEFEVAKVFKNHDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQA 153
           MGTSN  IFK F ASLTEFEVAKVF N+D+DGDGRLTKEELKSAFK LGYKFT  RVHQA
Sbjct: 1   MGTSNASIFKHFSASLTEFEVAKVFNNYDRDGDGRLTKEELKSAFKCLGYKFTIIRVHQA 60

Query: 154 LHAADDNCDDVISFEEMSKLIMYTAGS 181
           LHAAD+NCDDVISFEE+SKLI+YTAGS
Sbjct: 61  LHAADNNCDDVISFEEISKLIIYTAGS 87

[Close]

BLAST of CsaV3_2G027840 vs. NCBI nr
Match: KAG6598376.1 (hypothetical protein SDJN03_08154, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 145.6 bits (366), Expect = 4.2e-31
Identity = 72/82 (87.80%), Postives = 77/82 (93.90%), Query Frame = 0


Query: 1  MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEA 60
          MGNSKSKTYKRVLVPFSEEQLA+VFKNHD DGDG LTKEELKQAF+YLGSRF +FRVEEA
Sbjct: 1  MGNSKSKTYKRVLVPFSEEQLASVFKNHDGDGDGKLTKEELKQAFDYLGSRFSSFRVEEA 60

Query: 61 LRAADTNGDGVISMDEMGKLIK 83
          LRAADT+GDG ISM EMGKLI+
Sbjct: 61 LRAADTDGDGFISMAEMGKLIQ 82

[Close]

BLAST of CsaV3_2G027840 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 7.5e-15
Identity = 52/166 (31.33%), Postives = 79/166 (47.59%), Query Frame = 0


Query: 15  PFSEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEALRAADTNGDGVISM 74
           P  EE++  VF+  D +GDG +++ EL   FE LG    +  +   +  AD +GDG IS+
Sbjct: 35  PTPEEEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADADGDGFISL 94

Query: 75  DEMGKLIKAKPLYTYYNPSMGTSNGLIFKCFPASLTEFEVAKVFKNHDKDGDGRLTKEEL 134
           DE   L               T++G       A+  E ++   F+  D DG+G ++  EL
Sbjct: 95  DEFAAL-------------NATASG------DAAAVEEDLRHAFRVFDADGNGTISAAEL 154

Query: 135 KSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLIMYTAGS 181
                 LG K T  +  + +   D N D +ISFEE  K++M   GS
Sbjct: 155 ARVLHGLGEKATVQQCRRMIEGVDQNGDGLISFEEF-KVMMAGGGS 180

[Close]

BLAST of CsaV3_2G027840 vs. ExPASy Swiss-Prot
Match: Q9AWK2 (Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica OX=39947 GN=CML11 PE=2 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 4.1e-13
Identity = 48/167 (28.74%), Postives = 79/167 (47.31%), Query Frame = 0


Query: 15  PFSEEQLA---NVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEALRAADTNGDGV 74
           P  ++QL     +F++ DR+GDG LT+ EL      LG +     ++  ++ ADTN +G+
Sbjct: 56  PLGDDQLGELREIFRSFDRNGDGSLTQLELGSLLRSLGLKPSTDELDSLIQRADTNSNGL 115

Query: 75  ISMDEMGKLIKAKPLYTYYNPSMGTSNGLIFKCFPASLTEFEVAKVFKNHDKDGDGRLTK 134
           I   E   L+  + LY                   A  +E ++ ++F   D+DG+G +T 
Sbjct: 116 IEFSEFVALVAPELLYD-----------------RAPYSEDQIRRLFNIFDRDGNGFITA 175

Query: 135 EELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLIMYTA 179
            EL  +   LG+  T   +   +  AD + D  ISF+E S+ I   A
Sbjct: 176 AELAHSMAKLGHALTVKELTGMIKEADTDGDGRISFQEFSRAITAAA 205

[Close]

BLAST of CsaV3_2G027840 vs. ExPASy Swiss-Prot
Match: Q9ZSA4 (Calcium-dependent protein kinase 27 OS=Arabidopsis thaliana OX=3702 GN=CPK27 PE=1 SV=3)

HSP 1 Score: 72.8 bits (177), Expect = 4.5e-12
Identity = 57/174 (32.76%), Postives = 82/174 (47.13%), Query Frame = 0


Query: 9   YKRVLVPF-----SEEQ---LANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEA 68
           +K+V++ F     SEE+   L  +F N D D  G++T EELK     LGS      VE+ 
Sbjct: 317 FKKVVLKFIAANLSEEEIKGLKTLFTNIDTDKSGNITLEELKTGLTRLGSNLSKTEVEQL 376

Query: 69  LRAADTNGDGVISMDEMGKLIKAKPLYTYYNPSMGTSNGLIFKCFPASLTEFEVAKVFKN 128
           + AAD +G+G I +DE    I A    T +   +                   V K F++
Sbjct: 377 MEAADMDGNGTIDIDE---FISA----TMHRYKLDRDE--------------HVYKAFQH 436

Query: 129 HDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLI 175
            DKD DG +TKEEL+ A K  G       + Q +  AD + D  I+FEE   ++
Sbjct: 437 FDKDNDGHITKEELEMAMKEDG-AGDEGSIKQIIADADTDNDGKINFEEFRTMM 468

[Close]

BLAST of CsaV3_2G027840 vs. ExPASy Swiss-Prot
Match: Q948R0 (Calmodulin-like protein 5 OS=Oryza sativa subsp. japonica OX=39947 GN=CML5 PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 4.5e-12
Identity = 51/167 (30.54%), Postives = 75/167 (44.91%), Query Frame = 0


Query: 12  VLVPFSEEQLA---NVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEALRAADTNG 71
           V V   +EQ+A     F   D+DGDG +T EEL      LG       + E +R  D +G
Sbjct: 4   VEVRVRQEQVAEFRETFAFFDKDGDGCITLEELDTVVRSLGQTPTREELAEMIRDVDVDG 63

Query: 72  DGVISMDEMGKLIKAKPLYTYYNPSMGTSNGLIFKCFPASLTEFEVAKVFKNHDKDGDGR 131
           +G I   E   L+  K      N   G  +G           + E+ + FK  DKD DG 
Sbjct: 64  NGTIEFAEFLALMARKASRGGENGGGGDDSG--------DAADEELREAFKVFDKDQDGL 123

Query: 132 LTKEELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLIM 176
           ++  EL+     LG K T   V Q +  AD + D  ++F+E  +++M
Sbjct: 124 ISAAELRHVMISLGEKLTDEEVEQMIREADLDGDGQVNFDEFVRMMM 162

[Close]

BLAST of CsaV3_2G027840 vs. ExPASy Swiss-Prot
Match: Q2RAV0 (Calcium-dependent protein kinase 25 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK25 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 5.9e-12
Identity = 52/189 (27.51%), Postives = 81/189 (42.86%), Query Frame = 0


Query: 1   MGNSKSKTYKRVLVPFSEEQ---LANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRV 60
           M   K    + +    SEE+   L  +FKN D+D  G +T EELK      G++F +  +
Sbjct: 366 MNQFKKAALRIIAGCLSEEEIKGLKEMFKNIDKDNSGTITLEELKNGLAKQGTKFSDNEI 425

Query: 61  EEALRAADTNGDGVISMDE-------MGKLIKAKPLYTYYNPSMGTSNGLIFKCFPASLT 120
           E+ + AAD +G+G+I  +E       M K+ + + LYT                      
Sbjct: 426 EQLMEAADADGNGIIDYEEFVTATVHMNKMDREEHLYT---------------------- 485

Query: 121 EFEVAKVFKNHDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEM 180
                  F+  DKD  G +TKEEL+ A K  G  +    +   +  AD N D  I + E 
Sbjct: 486 ------AFQYFDKDNSGYITKEELEQALKEQGL-YDANEIKDVITDADSNNDGRIDYSEF 525

[Close]

BLAST of CsaV3_2G027840 vs. ExPASy TrEMBL
Match: A0A0A0LP43 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357350 PE=4 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 8.8e-43
Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = 0


Query: 94  MGTSNGLIFKCFPASLTEFEVAKVFKNHDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQA 153
           MGTSNGLIFKCFPASLTEFEVAKVFKNHDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQA
Sbjct: 1   MGTSNGLIFKCFPASLTEFEVAKVFKNHDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQA 60

Query: 154 LHAADDNCDDVISFEEMSKLIMYTAGS 181
           LHAADDNCDDVISFEEMSKLIMYTAGS
Sbjct: 61  LHAADDNCDDVISFEEMSKLIMYTAGS 87

[Close]

BLAST of CsaV3_2G027840 vs. ExPASy TrEMBL
Match: A0A0A0LRB6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357340 PE=4 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 1.7e-38
Identity = 82/82 (100.00%), Postives = 82/82 (100.00%), Query Frame = 0


Query: 1  MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEA 60
          MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEA
Sbjct: 1  MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEA 60

Query: 61 LRAADTNGDGVISMDEMGKLIK 83
          LRAADTNGDGVISMDEMGKLIK
Sbjct: 61 LRAADTNGDGVISMDEMGKLIK 82

[Close]

BLAST of CsaV3_2G027840 vs. ExPASy TrEMBL
Match: A0A5A7V5B0 (Putative Calcium-binding EF-hand family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00320 PE=4 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 1.6e-36
Identity = 80/82 (97.56%), Postives = 81/82 (98.78%), Query Frame = 0


Query: 1  MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEA 60
          MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDG+LTKEELKQAFEYLGSRF NFRVEEA
Sbjct: 1  MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGNLTKEELKQAFEYLGSRFSNFRVEEA 60

Query: 61 LRAADTNGDGVISMDEMGKLIK 83
          LRAADTNGDGVISMDEMGKLIK
Sbjct: 61 LRAADTNGDGVISMDEMGKLIK 82

[Close]

BLAST of CsaV3_2G027840 vs. ExPASy TrEMBL
Match: A0A5A7V027 (Putative Calcium-binding EF-hand family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00310 PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 1.7e-33
Identity = 74/87 (85.06%), Postives = 79/87 (90.80%), Query Frame = 0


Query: 94  MGTSNGLIFKCFPASLTEFEVAKVFKNHDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQA 153
           MGTSN  IFK F ASLTEFEVAKVF N+D+DGDGRLTKEELKSAFK LGYKFT  RVHQA
Sbjct: 1   MGTSNASIFKHFSASLTEFEVAKVFNNYDRDGDGRLTKEELKSAFKCLGYKFTIIRVHQA 60

Query: 154 LHAADDNCDDVISFEEMSKLIMYTAGS 181
           LHAAD+NCDDVISFEE+SKLI+YTAGS
Sbjct: 61  LHAADNNCDDVISFEEISKLIIYTAGS 87

[Close]

BLAST of CsaV3_2G027840 vs. ExPASy TrEMBL
Match: A0A7N2KRS3 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 4.1e-32
Identity = 75/172 (43.60%), Postives = 110/172 (63.95%), Query Frame = 0


Query: 5   KSKTYKRVLVPFSEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEALRAA 64
           K   YK   V ++++QL NVFK++D DGDG L++EELK+AF+YLGSR+ ++R ++ALR  
Sbjct: 150 KVTVYKPTSVLYTKDQLMNVFKSYDADGDGKLSREELKKAFKYLGSRWGSYRADQALRCT 209

Query: 65  DTNGDGVISMDEMGKLIKAKPLYTYYNPSMGTSNGLIFKCFPASLTEFEVAKVFKNHDKD 124
           D N DG I   E+ KL+     YT     +     + FK       E ++  +FK+HD D
Sbjct: 210 DANDDGYIDEKELEKLVD----YT-----LERRKAMAFKRTSVPFNEQQLLNIFKSHDVD 269

Query: 125 GDGRLTKEELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLIMY 177
           GDG+L+++ELK+AFKYLG ++  +R  QAL  AD N D  I+ +E+ KL+ Y
Sbjct: 270 GDGKLSRDELKNAFKYLGSRWDAYRTDQALRQADANDDGYITEDELKKLVEY 312

[Close]

BLAST of CsaV3_2G027840 vs. TAIR 10
Match: AT4G04700.1 (calcium-dependent protein kinase 27 )

HSP 1 Score: 72.8 bits (177), Expect = 3.2e-13
Identity = 57/174 (32.76%), Postives = 82/174 (47.13%), Query Frame = 0


Query: 9   YKRVLVPF-----SEEQ---LANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEA 68
           +K+V++ F     SEE+   L  +F N D D  G++T EELK     LGS      VE+ 
Sbjct: 317 FKKVVLKFIAANLSEEEIKGLKTLFTNIDTDKSGNITLEELKTGLTRLGSNLSKTEVEQL 376

Query: 69  LRAADTNGDGVISMDEMGKLIKAKPLYTYYNPSMGTSNGLIFKCFPASLTEFEVAKVFKN 128
           + AAD +G+G I +DE    I A    T +   +                   V K F++
Sbjct: 377 MEAADMDGNGTIDIDE---FISA----TMHRYKLDRDE--------------HVYKAFQH 436

Query: 129 HDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLI 175
            DKD DG +TKEEL+ A K  G       + Q +  AD + D  I+FEE   ++
Sbjct: 437 FDKDNDGHITKEELEMAMKEDG-AGDEGSIKQIIADADTDNDGKINFEEFRTMM 468

[Close]

BLAST of CsaV3_2G027840 vs. TAIR 10
Match: AT4G04695.1 (calcium-dependent protein kinase 31 )

HSP 1 Score: 71.6 bits (174), Expect = 7.2e-13
Identity = 55/177 (31.07%), Postives = 78/177 (44.07%), Query Frame = 0


Query: 1   MGNSKSKTYKRVLVPFSEEQ---LANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRV 60
           M   K    K +    SEE+   L  +F N D D  G +T EELK     LGS      V
Sbjct: 314 MNKLKKVALKVIAANLSEEEIKGLKTLFTNIDTDKSGTITLEELKTGLTRLGSNLSKTEV 373

Query: 61  EEALRAADTNGDGVISMDEMGKLIKAKPLYTYYNPSMGTSNGLIFKCFPASLTEFEVAKV 120
           E+ + AAD +G+G I +DE    I A    T +   +   +               V + 
Sbjct: 374 EQLMEAADVDGNGTIDIDE---FISA----TMHRYRLDRDD--------------HVYQA 433

Query: 121 FKNHDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLI 175
           F++ DKD DG +TKEEL+ A K  G       + Q +   D + D  I+FEE   ++
Sbjct: 434 FQHFDKDNDGHITKEELEMAMKEHGVG-DEVSIKQIITEVDTDNDGKINFEEFRTMM 468

[Close]

BLAST of CsaV3_2G027840 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 67.8 bits (164), Expect = 1.0e-11
Identity = 41/157 (26.11%), Postives = 74/157 (47.13%), Query Frame = 0


Query: 20  QLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEALRAADTNGDGVISMDEMGK 79
           +L+ VF+  D++GDG + K ELK  F+ +G       + E +   D NGDG + +DE G 
Sbjct: 5   ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGS 64

Query: 80  LIKAKPLYTYYNPSMGTSNGLIFKCFPASLTEFEVAKVFKNHDKDGDGRLTKEELKSAFK 139
           L +                    +       E ++ + F+  D++GDG +T EEL+S   
Sbjct: 65  LYQ--------------------EMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLA 124

Query: 140 YLGYK--FTTFRVHQALHAADDNCDDVISFEEMSKLI 175
            +G K   T     + +   D + D +++F+E  +++
Sbjct: 125 SMGLKQGRTLEDCKKMISKVDVDGDGMVNFKEFKQMM 141

[Close]

BLAST of CsaV3_2G027840 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 67.0 bits (162), Expect = 1.8e-11
Identity = 45/162 (27.78%), Postives = 76/162 (46.91%), Query Frame = 0


Query: 16  FSEEQ---LANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEALRAADTNGDGVI 75
           F EE+   L  VF   D + DG ++ EELK++F+ LG +  +   E A++ +D +GDG++
Sbjct: 15  FLEEKNRDLEAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGML 74

Query: 76  SMDEMGKLIKAKPLYTYYNPSMGTSNGLIFKCFPASLTEFEVAKVFKNHDKDGDGRLTKE 135
            ++E   LIK    +T                        ++ + F+ +  DG+  +T  
Sbjct: 75  DINEFALLIKGNDEFTEEEKKR------------------KIMEAFRMYIADGEDCITPG 134

Query: 136 ELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLI 175
            LK     LG   TT      + A D N D V+SF+E + ++
Sbjct: 135 SLKMMLMKLGESRTTDDCKVMIQAFDLNADGVLSFDEFALMM 158

[Close]

BLAST of CsaV3_2G027840 vs. TAIR 10
Match: AT4G04720.1 (calcium-dependent protein kinase 21 )

HSP 1 Score: 66.2 bits (160), Expect = 3.0e-11
Identity = 51/177 (28.81%), Postives = 75/177 (42.37%), Query Frame = 0


Query: 1   MGNSKSKTYKRVLVPFSEEQ---LANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRV 60
           M   K    K +    SEE+   L  +F N D D  G +T EELK     LGSR     V
Sbjct: 362 MNKLKKLALKVIAESLSEEEIKGLKTMFANIDTDKSGTITYEELKTGLTRLGSRLSETEV 421

Query: 61  EEALRAADTNGDGVISMDEMGKLIKAKPLYTYYNPSMGTSNGLIFKCFPASLTEFEVAKV 120
           ++ + AAD +G+G I                YY     T +            +  V K 
Sbjct: 422 KQLMEAADVDGNGTID---------------YYEFISATMHRYKLD------RDEHVYKA 481

Query: 121 FKNHDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLI 175
           F++ DKD  G +T++EL+SA K  G       + + +   D + D  I+FEE   ++
Sbjct: 482 FQHFDKDNSGHITRDELESAMKEYGMG-DEASIKEVISEVDTDNDGRINFEEFCAMM 516

The following BLAST results are available for this feature:

BLAST of CsaV3_2G027840 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN62765.2	1.6e-99	100.00	hypothetical protein Csa_022396 [Cucumis sativus]	[more]
KAG7020566.1	5.6e-52	63.84	hypothetical protein SDJN02_17252, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAA0060899.1	3.3e-36	97.56	putative Calcium-binding EF-hand family protein [Cucumis melo var. makuwa]	[more]
KAA0060898.1	3.4e-33	85.06	putative Calcium-binding EF-hand family protein [Cucumis melo var. makuwa]	[more]
KAG6598376.1	4.2e-31	87.80	hypothetical protein SDJN03_08154, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of CsaV3_2G027840 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RZB5	7.5e-15	31.33	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9AWK2	4.1e-13	28.74	Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9ZSA4	4.5e-12	32.76	Calcium-dependent protein kinase 27 OS=Arabidopsis thaliana OX=3702 GN=CPK27 PE=...	[more]
Q948R0	4.5e-12	30.54	Calmodulin-like protein 5 OS=Oryza sativa subsp. japonica OX=39947 GN=CML5 PE=2 ...	[more]
Q2RAV0	5.9e-12	27.51	Calcium-dependent protein kinase 25 OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]

BLAST of CsaV3_2G027840 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LP43	8.8e-43	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357350 PE=4 SV=1	[more]
A0A0A0LRB6	1.7e-38	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357340 PE=4 SV=1	[more]
A0A5A7V5B0	1.6e-36	97.56	Putative Calcium-binding EF-hand family protein OS=Cucumis melo var. makuwa OX=1...	[more]
A0A5A7V027	1.7e-33	85.06	Putative Calcium-binding EF-hand family protein OS=Cucumis melo var. makuwa OX=1...	[more]
A0A7N2KRS3	4.1e-32	43.60	Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1	[more]

BLAST of CsaV3_2G027840 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G04700.1	3.2e-13	32.76	calcium-dependent protein kinase 27	[more]
AT4G04695.1	7.2e-13	31.07	calcium-dependent protein kinase 31	[more]
AT4G12860.1	1.0e-11	26.11	EF hand calcium-binding protein family	[more]
AT1G76640.1	1.8e-11	27.78	Calcium-binding EF-hand family protein	[more]
AT4G04720.1	3.0e-11	28.81	calcium-dependent protein kinase 21	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 56..84 e-value: 4.6E-4 score: 29.5 coord: 20..48 e-value: 0.083 score: 22.0 coord: 113..141 e-value: 0.0012 score: 28.1 coord: 149..177 e-value: 17.0 score: 7.9
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 115..174 e-value: 3.7E-10 score: 40.1 coord: 18..80 e-value: 1.5E-12 score: 47.8
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 109..144 score: 15.461849
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 52..87 score: 10.663839
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 16..51 score: 15.657116
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 113..175 e-value: 3.17043E-11 score: 54.4761
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 20..82 e-value: 1.93789E-13 score: 60.2541
None	No IPR available	GENE3D	1.10.238.10		coord: 98..178 e-value: 7.3E-14 score: 54.1
None	No IPR available	GENE3D	1.10.238.10		coord: 3..86 e-value: 2.6E-15 score: 58.8
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 107..176 coord: 10..83
None	No IPR available	PANTHER	PTHR10891:SF911	SUBFAMILY NOT NAMED	coord: 107..176 coord: 10..83
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 158..170
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 29..41
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 122..134
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 65..77
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 18..174

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_2G027840.1	CsaV3_2G027840.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding