CsaV3_2G025540 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_2G025540
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionBeta-fructofuranosidase, insoluble isoenzyme CWINV3-like
Locationchr2: 17555902 .. 17559448 (-)
RNA-Seq ExpressionCsaV3_2G025540
SyntenyCsaV3_2G025540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CAATTAGAAAAAAAAAATGTTAATGATAATGTTTTCTTAAGTTTTATGTTAATTTTTCCGTAAGGAAGATTTGAATCTCTAGTCTTTTATTTGAAATTATGATATTAGAATGATTAAACTATATTTAGATTAGACAAGATGAACTATCGTGTGTGTATATATATAAACGTAATGGAAGCTAGAATTGTATGCATGTTTTTTGAAATGAATACTACTAATCTTTTGTTTGATAAAATGCCACCAAAAAGGTTAGAAATAGGCAAGTATGGTTTAGTTGTATCATTTGTTATTTTGCTTACATGCAATAGAATTGGATTTGTGGTTTCTCATGAATATGCCAATGAAGTTCATACTCCACAAGTTCAAAGGACATCTTATCACTTCCAACCCCTCAAAAATTGGATGAATGGTAATACATATATACATACAATGTATATATTCTTCCTATTTGTTTCGCTATTTTTTCTCAAAAAAAAAATCGTTTGAATATCGATCTCTACTTTATTACATATTCTAACTTTGTTATTTGTATTTTTTTTTTCTCCCTTGATCGAAATTTCATTTGATGGATGAATCATCAATATGCTTTACGATATCGTAAAGACCCAAATGGTAAGAATTGTTTAAAGCTTTATAGCTATATGTATAATAATTAAACTTTAGGTATCTCTTTCTAATATAAGTTTATCGACAATAATATAATAAGAGTGTTGATTCAAATTACATTTGATCCTTCGATATGAAATTCAAACTTACAAAGAGAAACGGTTATCAATATTAGGTGAGTAGTATAGGCTATGATAATGAAACTAAAAAAAACATCTGATGAATGAGTAGAAACCATGAAATAAAATACAATAAATTTGCCTAATTATTCGTTAAAAAGTATGATTTTTTTGCAATTTGTTGATTGAAAGGACCAATGTTCTACAAGGGAATTTACCACTTTTTCTATCAACACAATCCCAATGGAGCTGTTTTCAATAGCAAAATGGTGTGGGCTCATTCAATATCATACGATCTAATCAATTGGGTCCATTTGAATCATGCCCTTGAGCCCACTGATCCATTTGATATCAATGGTTGCTGGTCCGGCTCAGTCTCCTTCCTCCCCGAGAACAAACCCCGCATTCTCTACACTGGAATTGATTCCAGTTCCCAGCAGGTTCAGAATTTAGCAATACCCGCCAATTATTCAGACCCTTTTCTTGAAAAGTGGAGAAAATTCTCCCAAAATCCAATTATTGCTCCTCCCGATGGGTTGGAAAGAAACAGATTTAGAGACCCAACTACAGCTTGGCAGGGCCCAGATGGAGAATGGAGGGTCGCCATTGGAGGGCAGACGAGCTATGGAGGAGCAGCGATGCTTTACAGAAGTGAGGACTTCGTTCGCTGGCATCAGTTCAGGTTTCCTCTTTATTCTTCTCAAGATTCTGGAACTTGGGAATGTCCTGATTTTTACCCTGTGATGCTTAATGGAACGAATGGGATTGGTTTCTCTAGTGGATTTGGGGTTGGTGTTAAGTATGTGATGAAAGCTAGCTTTAATTCTAGTGATCATTATACATTGGGAAGTTATGTCCCTGAGAAAGAGAAGTTCACTTCTGATTATGGTCCTGGGTTTGACTTTAAAGGAATAAATTTAGGGTTGAGATATGATTATGGAAAGTTTTATGCTTCCAAGACGTTTTATGATAGTTCCCAAAAGAGGAGGATATTGTGGGGTTGGGTTAATGAATCTGATAGCAGACAAGATGATATCAATAAGGGATGGTCTGGACTTCAGGTAAATTTTGGAATGTTTGTAAATTATGATCCATTTTCATGTCTTAGATTATGGAAGTAGGAATTGGAATGAATCTTGGTTCTTAATTTTCGAAATGCAGGCGATTCCTCGAAAGATTTGGCTATCCAAGACGGGAAGGCAGTTGATACAATGGCCAGTCAAGGAAATTAAGATGTTACGCAGGAACCATTTTAGCTTGCATCATAAAGAACTTAGAGGGAGATCAACAATGGAAGTTCTTGGTGGCTCAGCTTCCCAGGTTCGTATTTGACACCGTGTGATCAATAGTTTCTGTGGAAGCCCTATTTGGAGATATAGTAGACATTATCAGTAGAAAATTAGTGCGTTTGTTAAAAGGGGGATATTAGGATATAGACAGTAAATTTGCTAGAGCTTTTAGTTTGGTTGTGAGGAAGGCGTGAGAATTCTGTGGAACTTTCATTGGTGAATTTGGGAGAGCAGAACCCTCTCTAAAGGCTACTGTGTATCGTAGTTTCTAATTTTCTTCATTGATATTGCAATGTATACTTCTTTCTTTGAGTGTTCTTTGTGCCCGCCCATTGTTGTTCTTGGCATTCTTGTTAGGTGTCATCCTATTAGACTCAACTATTGTTCATTATCGAGATTTTTCTTGATTTGATTGTATGATTGATCAAATGGTTCAAGGTGGACGTGGAGGTTTCCTTTGAGCTTCCATACTTAGAGGAGGCAGAGTCAGTGGACACGAGATGGGGTGTTCTTGATCCTCAAGTGCTGTGTAGCAAAAGAGATGCATCAGTAAATGGCAGAGTTGGACCCTTTGGCTTGTTGGTTTTGGCCTCCAATGACTTATCTGAACACACTGCAATATATTTTCATATTCTCAAAGCTCATAACAGATATGTAGTGCTTATGTGCAGTGATCAAAGCAGGTTAAATCCATGCCCATGGCTTTCTATTTACAAGCATTTTTTAAGTTCTTTTTTCAGAATGCAAACCCAACTGATTGGCTGTTTCTCTTACACTGATAGGTCTTCTTTCAGAAAGGAGGTAGATAAAACCGCTCATGGGGCGTTTGTAGACATAGACCCATGCTACAAAAAGATATCACTGAGAACACTGGTATTTCCCTATTAGTTTGTGTTTGTTTTTCATTCGCTTAGGTTGCTTTCGTTTCTCATTTTTTGCCATCAAACTTTGCAGGTAGACCACTCTATAGTCGAAAGCTTTGGAGGAAAAGGAAAAACATGCATCACATCCCGAGTATATCCGACTTTGGCTGTCAATAACAATGCTCGTTTATACGCATTCAACAATGGAACTCAAACTGTAGTGATTTCCAGTTTGAAGGCTTGGAACATGTCAAATGCAGAAATCAAATGAGTTTCTGCAGCCGCCATGTCCCTTTTTCTTATCAAAATAAAGAAGCTACTAAGGGATGAAATTTGTTTGTAATTTTCTATATCTGAACCAAAAATGGATTAATTCACTATAATTATTGCTTATTTGATCAGCAATCATAAGTTAGACTTAAAAGAGCTATGCATTTGTACTTCTGACCTTCCACTGCCGTTTATTTAGGTCATTACCGCCAAATGCTTTTTAAGAAACTTTGTATTGTGCTTTCTTTGGGCATCCTCTCTTACATCTTTTTCATCAATCGTCGTCTTCCTCTTCTACGCAGAAATGTTATCTTATGTCATTGAATTAAAAAGAGAAAAATATTGGCTTTTTTCCTAAATTTAGGATTTGAAATTTTGCTGACATTGCTGAGTTTGGGCG

mRNA sequence

ATGAACTATCGTGTGTGTATATATATAAACGTAATGGAAGCTAGAATTGTATGCATGTTTTTTGAAATGAATACTACTAATCTTTTGTTTGATAAAATGCCACCAAAAAGGTTAGAAATAGGCAAGTATGGTTTAGTTGTATCATTTGTTATTTTGCTTACATGCAATAGAATTGGATTTGTGGTTTCTCATGAATATGCCAATGAAGTTCATACTCCACAAGTTCAAAGGACATCTTATCACTTCCAACCCCTCAAAAATTGGATGAATGACCCAAATGGACCAATGTTCTACAAGGGAATTTACCACTTTTTCTATCAACACAATCCCAATGGAGCTGTTTTCAATAGCAAAATGGTGTGGGCTCATTCAATATCATACGATCTAATCAATTGGGTCCATTTGAATCATGCCCTTGAGCCCACTGATCCATTTGATATCAATGGTTGCTGGTCCGGCTCAGTCTCCTTCCTCCCCGAGAACAAACCCCGCATTCTCTACACTGGAATTGATTCCAGTTCCCAGCAGGTTCAGAATTTAGCAATACCCGCCAATTATTCAGACCCTTTTCTTGAAAAGTGGAGAAAATTCTCCCAAAATCCAATTATTGCTCCTCCCGATGGGTTGGAAAGAAACAGATTTAGAGACCCAACTACAGCTTGGCAGGGCCCAGATGGAGAATGGAGGGTCGCCATTGGAGGGCAGACGAGCTATGGAGGAGCAGCGATGCTTTACAGAAGTGAGGACTTCGTTCGCTGGCATCAGTTCAGGTTTCCTCTTTATTCTTCTCAAGATTCTGGAACTTGGGAATGTCCTGATTTTTACCCTGTGATGCTTAATGGAACGAATGGGATTGGTTTCTCTAGTGGATTTGGGGTTGGTGTTAAGTATGTGATGAAAGCTAGCTTTAATTCTAGTGATCATTATACATTGGGAAGTTATGTCCCTGAGAAAGAGAAGTTCACTTCTGATTATGGTCCTGGGTTTGACTTTAAAGGAATAAATTTAGGGTTGAGATATGATTATGGAAAGTTTTATGCTTCCAAGACGTTTTATGATAGTTCCCAAAAGAGGAGGATATTGTGGGGTTGGGTTAATGAATCTGATAGCAGACAAGATGATATCAATAAGGGATGGTCTGGACTTCAGGCGATTCCTCGAAAGATTTGGCTATCCAAGACGGGAAGGCAGTTGATACAATGGCCAGTCAAGGAAATTAAGATGTTACGCAGGAACCATTTTAGCTTGCATCATAAAGAACTTAGAGGGAGATCAACAATGGAAGTTCTTGGTGGCTCAGCTTCCCAGGTGGACGTGGAGGTTTCCTTTGAGCTTCCATACTTAGAGGAGGCAGAGTCAGTGGACACGAGATGGGGTGTTCTTGATCCTCAAGTGCTGTGTAGCAAAAGAGATGCATCAGTAAATGGCAGAGTTGGACCCTTTGGCTTGTTGGTTTTGGCCTCCAATGACTTATCTGAACACACTGCAATATATTTTCATATTCTCAAAGCTCATAACAGATATGTAGTGCTTATGTGCAGTGATCAAAGCAGGTCTTCTTTCAGAAAGGAGGTAGATAAAACCGCTCATGGGGCGTTTGTAGACATAGACCCATGCTACAAAAAGATATCACTGAGAACACTGGTAGACCACTCTATAGTCGAAAGCTTTGGAGGAAAAGGAAAAACATGCATCACATCCCGAGTATATCCGACTTTGGCTGTCAATAACAATGCTCGTTTATACGCATTCAACAATGGAACTCAAACTGTAGTGATTTCCAGTTTGAAGGCTTGGAACATGTCAAATGCAGAAATCAAATGA

Coding sequence (CDS)

ATGAACTATCGTGTGTGTATATATATAAACGTAATGGAAGCTAGAATTGTATGCATGTTTTTTGAAATGAATACTACTAATCTTTTGTTTGATAAAATGCCACCAAAAAGGTTAGAAATAGGCAAGTATGGTTTAGTTGTATCATTTGTTATTTTGCTTACATGCAATAGAATTGGATTTGTGGTTTCTCATGAATATGCCAATGAAGTTCATACTCCACAAGTTCAAAGGACATCTTATCACTTCCAACCCCTCAAAAATTGGATGAATGACCCAAATGGACCAATGTTCTACAAGGGAATTTACCACTTTTTCTATCAACACAATCCCAATGGAGCTGTTTTCAATAGCAAAATGGTGTGGGCTCATTCAATATCATACGATCTAATCAATTGGGTCCATTTGAATCATGCCCTTGAGCCCACTGATCCATTTGATATCAATGGTTGCTGGTCCGGCTCAGTCTCCTTCCTCCCCGAGAACAAACCCCGCATTCTCTACACTGGAATTGATTCCAGTTCCCAGCAGGTTCAGAATTTAGCAATACCCGCCAATTATTCAGACCCTTTTCTTGAAAAGTGGAGAAAATTCTCCCAAAATCCAATTATTGCTCCTCCCGATGGGTTGGAAAGAAACAGATTTAGAGACCCAACTACAGCTTGGCAGGGCCCAGATGGAGAATGGAGGGTCGCCATTGGAGGGCAGACGAGCTATGGAGGAGCAGCGATGCTTTACAGAAGTGAGGACTTCGTTCGCTGGCATCAGTTCAGGTTTCCTCTTTATTCTTCTCAAGATTCTGGAACTTGGGAATGTCCTGATTTTTACCCTGTGATGCTTAATGGAACGAATGGGATTGGTTTCTCTAGTGGATTTGGGGTTGGTGTTAAGTATGTGATGAAAGCTAGCTTTAATTCTAGTGATCATTATACATTGGGAAGTTATGTCCCTGAGAAAGAGAAGTTCACTTCTGATTATGGTCCTGGGTTTGACTTTAAAGGAATAAATTTAGGGTTGAGATATGATTATGGAAAGTTTTATGCTTCCAAGACGTTTTATGATAGTTCCCAAAAGAGGAGGATATTGTGGGGTTGGGTTAATGAATCTGATAGCAGACAAGATGATATCAATAAGGGATGGTCTGGACTTCAGGCGATTCCTCGAAAGATTTGGCTATCCAAGACGGGAAGGCAGTTGATACAATGGCCAGTCAAGGAAATTAAGATGTTACGCAGGAACCATTTTAGCTTGCATCATAAAGAACTTAGAGGGAGATCAACAATGGAAGTTCTTGGTGGCTCAGCTTCCCAGGTGGACGTGGAGGTTTCCTTTGAGCTTCCATACTTAGAGGAGGCAGAGTCAGTGGACACGAGATGGGGTGTTCTTGATCCTCAAGTGCTGTGTAGCAAAAGAGATGCATCAGTAAATGGCAGAGTTGGACCCTTTGGCTTGTTGGTTTTGGCCTCCAATGACTTATCTGAACACACTGCAATATATTTTCATATTCTCAAAGCTCATAACAGATATGTAGTGCTTATGTGCAGTGATCAAAGCAGGTCTTCTTTCAGAAAGGAGGTAGATAAAACCGCTCATGGGGCGTTTGTAGACATAGACCCATGCTACAAAAAGATATCACTGAGAACACTGGTAGACCACTCTATAGTCGAAAGCTTTGGAGGAAAAGGAAAAACATGCATCACATCCCGAGTATATCCGACTTTGGCTGTCAATAACAATGCTCGTTTATACGCATTCAACAATGGAACTCAAACTGTAGTGATTTCCAGTTTGAAGGCTTGGAACATGTCAAATGCAGAAATCAAATGA

Protein sequence

MNYRVCIYINVMEARIVCMFFEMNTTNLLFDKMPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVHTPQVQRTSYHFQPLKNWMNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIAPPDGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLGSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHNRYVVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEIK*
Homology
BLAST of CsaV3_2G025540 vs. NCBI nr
Match: XP_004142928.3 (beta-fructofuranosidase, insoluble isoenzyme CWINV3 isoform X1 [Cucumis sativus] >KAE8652051.1 hypothetical protein Csa_018697 [Cucumis sativus])

HSP 1 Score: 1273.5 bits (3294), Expect = 0.0e+00
Identity = 607/607 (100.00%), Postives = 607/607 (100.00%), Query Frame = 0

Query: 1   MNYRVCIYINVMEARIVCMFFEMNTTNLLFDKMPPKRLEIGKYGLVVSFVILLTCNRIGF 60
           MNYRVCIYINVMEARIVCMFFEMNTTNLLFDKMPPKRLEIGKYGLVVSFVILLTCNRIGF
Sbjct: 1   MNYRVCIYINVMEARIVCMFFEMNTTNLLFDKMPPKRLEIGKYGLVVSFVILLTCNRIGF 60

Query: 61  VVSHEYANEVHTPQVQRTSYHFQPLKNWMNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMV 120
           VVSHEYANEVHTPQVQRTSYHFQPLKNWMNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMV
Sbjct: 61  VVSHEYANEVHTPQVQRTSYHFQPLKNWMNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMV 120

Query: 121 WAHSISYDLINWVHLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNL 180
           WAHSISYDLINWVHLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNL
Sbjct: 121 WAHSISYDLINWVHLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNL 180

Query: 181 AIPANYSDPFLEKWRKFSQNPIIAPPDGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGG 240
           AIPANYSDPFLEKWRKFSQNPIIAPPDGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGG
Sbjct: 181 AIPANYSDPFLEKWRKFSQNPIIAPPDGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGG 240

Query: 241 AAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKYVMK 300
           AAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKYVMK
Sbjct: 241 AAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKYVMK 300

Query: 301 ASFNSSDHYTLGSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQKRRI 360
           ASFNSSDHYTLGSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQKRRI
Sbjct: 301 ASFNSSDHYTLGSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQKRRI 360

Query: 361 LWGWVNESDSRQDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKE 420
           LWGWVNESDSRQDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKE
Sbjct: 361 LWGWVNESDSRQDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKE 420

Query: 421 LRGRSTMEVLGGSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNGRVGP 480
           LRGRSTMEVLGGSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNGRVGP
Sbjct: 421 LRGRSTMEVLGGSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNGRVGP 480

Query: 481 FGLLVLASNDLSEHTAIYFHILKAHNRYVVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCY 540
           FGLLVLASNDLSEHTAIYFHILKAHNRYVVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCY
Sbjct: 481 FGLLVLASNDLSEHTAIYFHILKAHNRYVVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCY 540

Query: 541 KKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNNARLYAFNNGTQTVVISSLKAWN 600
           KKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNNARLYAFNNGTQTVVISSLKAWN
Sbjct: 541 KKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNNARLYAFNNGTQTVVISSLKAWN 600

Query: 601 MSNAEIK 608
           MSNAEIK
Sbjct: 601 MSNAEIK 607

BLAST of CsaV3_2G025540 vs. NCBI nr
Match: XP_011649529.2 (beta-fructofuranosidase, insoluble isoenzyme CWINV3 isoform X2 [Cucumis sativus])

HSP 1 Score: 1260.7 bits (3261), Expect = 0.0e+00
Identity = 604/607 (99.51%), Postives = 604/607 (99.51%), Query Frame = 0

Query: 1   MNYRVCIYINVMEARIVCMFFEMNTTNLLFDKMPPKRLEIGKYGLVVSFVILLTCNRIGF 60
           MNYRVCIYINVMEARIVCMFFEMNTTNLLFDKMPPKRLEIGKYGLVVSFVILLTCNRIGF
Sbjct: 1   MNYRVCIYINVMEARIVCMFFEMNTTNLLFDKMPPKRLEIGKYGLVVSFVILLTCNRIGF 60

Query: 61  VVSHEYANEVHTPQVQRTSYHFQPLKNWMNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMV 120
           VVSHEYANEVHTPQVQRTSYHFQPLKNWM   NGPMFYKGIYHFFYQHNPNGAVFNSKMV
Sbjct: 61  VVSHEYANEVHTPQVQRTSYHFQPLKNWM---NGPMFYKGIYHFFYQHNPNGAVFNSKMV 120

Query: 121 WAHSISYDLINWVHLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNL 180
           WAHSISYDLINWVHLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNL
Sbjct: 121 WAHSISYDLINWVHLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNL 180

Query: 181 AIPANYSDPFLEKWRKFSQNPIIAPPDGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGG 240
           AIPANYSDPFLEKWRKFSQNPIIAPPDGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGG
Sbjct: 181 AIPANYSDPFLEKWRKFSQNPIIAPPDGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGG 240

Query: 241 AAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKYVMK 300
           AAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKYVMK
Sbjct: 241 AAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKYVMK 300

Query: 301 ASFNSSDHYTLGSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQKRRI 360
           ASFNSSDHYTLGSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQKRRI
Sbjct: 301 ASFNSSDHYTLGSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQKRRI 360

Query: 361 LWGWVNESDSRQDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKE 420
           LWGWVNESDSRQDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKE
Sbjct: 361 LWGWVNESDSRQDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKE 420

Query: 421 LRGRSTMEVLGGSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNGRVGP 480
           LRGRSTMEVLGGSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNGRVGP
Sbjct: 421 LRGRSTMEVLGGSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNGRVGP 480

Query: 481 FGLLVLASNDLSEHTAIYFHILKAHNRYVVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCY 540
           FGLLVLASNDLSEHTAIYFHILKAHNRYVVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCY
Sbjct: 481 FGLLVLASNDLSEHTAIYFHILKAHNRYVVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCY 540

Query: 541 KKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNNARLYAFNNGTQTVVISSLKAWN 600
           KKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNNARLYAFNNGTQTVVISSLKAWN
Sbjct: 541 KKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNNARLYAFNNGTQTVVISSLKAWN 600

Query: 601 MSNAEIK 608
           MSNAEIK
Sbjct: 601 MSNAEIK 604

BLAST of CsaV3_2G025540 vs. NCBI nr
Match: ALS30215.1 (cell wall invertase 4 [Cucumis sativus])

HSP 1 Score: 1209.1 bits (3127), Expect = 0.0e+00
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 0

Query: 33  MPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVHTPQVQRTSYHFQPLKNWMNDP 92
           MPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVHTPQVQRTSYHFQPLKNWMNDP
Sbjct: 1   MPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVHTPQVQRTSYHFQPLKNWMNDP 60

Query: 93  NGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDINGCWS 152
           NGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDINGCWS
Sbjct: 61  NGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDINGCWS 120

Query: 153 GSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIAPPDGLERN 212
           GSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIAPPDGLERN
Sbjct: 121 GSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIAPPDGLERN 180

Query: 213 RFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECP 272
           RFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECP
Sbjct: 181 RFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECP 240

Query: 273 DFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLGSYVPEKEKFTSDYGPGFDFK 332
           DFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLGSYVPEKEKFTSDYGPGFDFK
Sbjct: 241 DFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLGSYVPEKEKFTSDYGPGFDFK 300

Query: 333 GINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRKIWLS 392
           GINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRKIWLS
Sbjct: 301 GINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRKIWLS 360

Query: 393 KTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPYLEEAE 452
           KTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPYLEEAE
Sbjct: 361 KTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPYLEEAE 420

Query: 453 SVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHNRYVVLM 512
           SVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHNRYVVLM
Sbjct: 421 SVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHNRYVVLM 480

Query: 513 CSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRVYPT 572
           CSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRVYPT
Sbjct: 481 CSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRVYPT 540

Query: 573 LAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEIK 608
           LAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEIK
Sbjct: 541 LAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEIK 575

BLAST of CsaV3_2G025540 vs. NCBI nr
Match: XP_008444460.1 (PREDICTED: LOW QUALITY PROTEIN: beta-fructofuranosidase, insoluble isoenzyme CWINV3-like [Cucumis melo])

HSP 1 Score: 1149.0 bits (2971), Expect = 0.0e+00
Identity = 548/579 (94.65%), Postives = 562/579 (97.06%), Query Frame = 0

Query: 31  DKMPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVH-TP-QVQRTSYHFQPLKNW 90
           DKMPPKRLE+GKYGLV SF++LL  NR+GFVVSHEYANEVH TP QVQRTSYHFQPLKNW
Sbjct: 2   DKMPPKRLEMGKYGLVASFILLLASNRMGFVVSHEYANEVHSTPQQVQRTSYHFQPLKNW 61

Query: 91  MNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDIN 150
           MNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDIN
Sbjct: 62  MNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDIN 121

Query: 151 GCWSGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIAPPDG 210
           GCWSGSVSFLP+NKPRILYTGIDSS QQVQNLAIP NYSDPFLEKWRK  QNPIIAPPDG
Sbjct: 122 GCWSGSVSFLPKNKPRILYTGIDSSFQQVQNLAIPTNYSDPFLEKWRKSPQNPIIAPPDG 181

Query: 211 LERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGT 270
            ERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGT
Sbjct: 182 AERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGT 241

Query: 271 WECPDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLGSYVPEKEKFTSDYGPG 330
           WECPDFYPVMLNGTNGIGFSSGFGVGVK+VMKASFNS DHYTLGSYVPEKEKFTSD+G G
Sbjct: 242 WECPDFYPVMLNGTNGIGFSSGFGVGVKHVMKASFNSRDHYTLGSYVPEKEKFTSDHGLG 301

Query: 331 FDFKGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRK 390
           FDF+GINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRK
Sbjct: 302 FDFRGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRK 361

Query: 391 IWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPYL 450
           IWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSF LP+L
Sbjct: 362 IWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFALPHL 421

Query: 451 EEAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHNRY 510
           EEAESVDTRWGVLDPQVLCSKRDAS+NGRVGPFGLLVLASNDLSEHTAIYFH+LKAHNRY
Sbjct: 422 EEAESVDTRWGVLDPQVLCSKRDASMNGRVGPFGLLVLASNDLSEHTAIYFHVLKAHNRY 481

Query: 511 VVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSR 570
           VVLMCSD+SRSSFRK VDKT +GAFVDIDP YKKISLRTLVDHSIVESFGGKGKTCITSR
Sbjct: 482 VVLMCSDESRSSFRKGVDKTTYGAFVDIDPRYKKISLRTLVDHSIVESFGGKGKTCITSR 541

Query: 571 VYPTLAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEIK 608
           VYPTLAVNN+ARLYAFNNGTQTVVISSLK WNMSNAEIK
Sbjct: 542 VYPTLAVNNDARLYAFNNGTQTVVISSLKVWNMSNAEIK 580

BLAST of CsaV3_2G025540 vs. NCBI nr
Match: KAA0061001.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV3-like [Cucumis melo var. makuwa])

HSP 1 Score: 983.8 bits (2542), Expect = 6.7e-283
Identity = 468/489 (95.71%), Postives = 477/489 (97.55%), Query Frame = 0

Query: 119 MVWAHSISYDLINWVHLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQ 178
           MVWAHSISYDLINWVHLNHALEPTDPFDINGCWSGSVSFLP+NKPRILYTGIDSS QQVQ
Sbjct: 1   MVWAHSISYDLINWVHLNHALEPTDPFDINGCWSGSVSFLPKNKPRILYTGIDSSFQQVQ 60

Query: 179 NLAIPANYSDPFLEKWRKFSQNPIIAPPDGLERNRFRDPTTAWQGPDGEWRVAIGGQTSY 238
           NLAIP NYSDPFLEKWRKF QNPIIAPPDG ERNRFRDPTTAWQGPDGEWRVAIGGQTSY
Sbjct: 61  NLAIPTNYSDPFLEKWRKFPQNPIIAPPDGAERNRFRDPTTAWQGPDGEWRVAIGGQTSY 120

Query: 239 GGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKYV 298
           GGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVK+V
Sbjct: 121 GGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKHV 180

Query: 299 MKASFNSSDHYTLGSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQKR 358
           MKASFNS DHYTLGSYVPEKEKFTSDYG GFDF+GINLGLRYDYGKFYASKTFYDSSQKR
Sbjct: 181 MKASFNSRDHYTLGSYVPEKEKFTSDYGLGFDFRGINLGLRYDYGKFYASKTFYDSSQKR 240

Query: 359 RILWGWVNESDSRQDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHH 418
           RILWGWVNESDSRQDDINKGWSGLQ IPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHH
Sbjct: 241 RILWGWVNESDSRQDDINKGWSGLQVIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHH 300

Query: 419 KELRGRSTMEVLGGSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNGRV 478
           KELRGRSTMEVLGGSASQVDVEVSF LP+LEEAESVDTRWGVLDPQVLCSKRDAS+NGRV
Sbjct: 301 KELRGRSTMEVLGGSASQVDVEVSFALPHLEEAESVDTRWGVLDPQVLCSKRDASMNGRV 360

Query: 479 GPFGLLVLASNDLSEHTAIYFHILKAHNRYVVLMCSDQSRSSFRKEVDKTAHGAFVDIDP 538
           GPFGLLVLASNDLSEHTAIYFH+LKAHNRYVVLMCSD+SRSSFRK VDKT +GAFVDIDP
Sbjct: 361 GPFGLLVLASNDLSEHTAIYFHVLKAHNRYVVLMCSDESRSSFRKGVDKTTYGAFVDIDP 420

Query: 539 CYKKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNNARLYAFNNGTQTVVISSLKA 598
            YKKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNN+ARLYAFNNGTQTVVISSLK 
Sbjct: 421 RYKKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNDARLYAFNNGTQTVVISSLKV 480

Query: 599 WNMSNAEIK 608
           WNMSNAEIK
Sbjct: 481 WNMSNAEIK 489

BLAST of CsaV3_2G025540 vs. ExPASy Swiss-Prot
Match: Q67XZ3 (Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3702 GN=CWINV3 PE=1 SV=2)

HSP 1 Score: 625.2 bits (1611), Expect = 7.9e-178
Identity = 304/571 (53.24%), Postives = 397/571 (69.53%), Query Frame = 0

Query: 47  VSFVILLTCNRIGFVVSHEYANEVHTPQVQRTSYHFQPLKNWMNDPNGPMFYKGIYHFFY 106
           +   +LL+     F+   E ++     Q  RT YHFQPLKNWMNDPNGPM YKGIYH FY
Sbjct: 9   IGLSLLLSMFLANFITDLEASSHQDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGIYHLFY 68

Query: 107 QHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDINGCWSGSVSFLPENKPRIL 166
           Q+NP GAV++ ++VW HS S DL+NW+    A  P+ P DINGCWSGSV+ LP  KP IL
Sbjct: 69  QYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSGSVTILPNGKPVIL 128

Query: 167 YTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIA--PPDGLERNRFRDPTTAWQGP 226
           YTGID +  QVQN+A+P N SDP+L +W K  QNP++     +G+  +RFRDPTTAW G 
Sbjct: 129 YTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINPDRFRDPTTAWLGR 188

Query: 227 DGEWRVAIGGQT-SYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTN 286
           DGEWRV +G  T    G A+LY+S DF  W Q   PL+    +G WECPDF+PV + G++
Sbjct: 189 DGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWECPDFFPVSITGSD 248

Query: 287 GIGFSSGFGVGVKYVMKASFNSS--DHYTLGSYVPEKEKFTSDYGPGFDFKGINLGLRYD 346
           G+  SS    G+K+V+K S   +  D+YT+GSY  EK+ +  D G    F       R D
Sbjct: 249 GVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDLG----FVQNESAPRLD 308

Query: 347 YGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRKIWLSKTGRQLIQW 406
           YGK+YASKTFYD  +KRRILWGWVNES   +DDI KGWSGLQ+ PRKIWL ++G++L+QW
Sbjct: 309 YGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLDESGKELLQW 368

Query: 407 PVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPYLEEAESVDTRWGVL 466
           P++EI+ LR    +   K L+  ST++V G +A+Q DVEVSF++  LE+A+ ++  W   
Sbjct: 369 PIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKADVIEPSW--T 428

Query: 467 DPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHN------RYVVLMCSD 526
           DPQ +CS+ D SV   +GPFGL+VLASND+ E+T++YF I K+++      +YVVLMCSD
Sbjct: 429 DPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCSD 488

Query: 527 QSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAV 586
           QSRSS   E DK+  GAFV IDP ++ ISLRTL+DHSIVES+GG G+TCITSRVYP LA+
Sbjct: 489 QSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTCITSRVYPKLAI 548

Query: 587 NNNARLYAFNNGTQTVVISSLKAWNMSNAEI 607
             NA L+ FN GTQ+V I +L AW++ +A+I
Sbjct: 549 GENANLFVFNKGTQSVDILTLSAWSLKSAQI 573

BLAST of CsaV3_2G025540 vs. ExPASy Swiss-Prot
Match: Q43866 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3702 GN=CWINV1 PE=1 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 3.8e-172
Identity = 299/544 (54.96%), Postives = 393/544 (72.24%), Query Frame = 0

Query: 74  QVQRTSYHFQPLKNWMNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWV 133
           Q  RT +HFQP KNWMNDPNGPM YKGIYH FYQ NP GAV+ + +VWAHS S DLINW 
Sbjct: 49  QPYRTGFHFQPPKNWMNDPNGPMIYKGIYHLFYQWNPKGAVWGN-IVWAHSTSTDLINWD 108

Query: 134 HLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEK 193
               A+ P+ PFDINGCWSGS + LP  KP ILYTGID  +QQVQN+A P N SDP+L +
Sbjct: 109 PHPPAIFPSAPFDINGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLRE 168

Query: 194 WRKFSQNPIIAPP--DGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFV 253
           W+K   NP++AP   +G+  + FRDPTTAW G D +WRV IG +    G A+ Y S+DF+
Sbjct: 169 WKKSPLNPLMAPDAVNGINASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFL 228

Query: 254 RWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVG---VKYVMKASFNSS-- 313
           +W +   PL+    SG WECPDF+PV   G+NG+  SS FG     +K+V+K S + +  
Sbjct: 229 KWEKSPEPLHYDDGSGMWECPDFFPVTRFGSNGVETSS-FGEPNEILKHVLKISLDDTKH 288

Query: 314 DHYTLGSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVN 373
           D+YT+G+Y   K+KF  D G    FK      RYDYGK+YASKTF+DS++ RRILWGW N
Sbjct: 289 DYYTIGTYDRVKDKFVPDNG----FKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTN 348

Query: 374 ESDSRQDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHF-SLHHKELRGRS 433
           ES S +DD+ KGWSG+Q IPRKIWL ++G+QLIQWPV+E++ LR     +L +K L+  S
Sbjct: 349 ESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGS 408

Query: 434 TMEVLGGSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLV 493
            +EV G +A+Q DVEV F++  LE+A+ ++  W   DPQ++CSK + SV   +GPFGL+V
Sbjct: 409 RLEVYGVTAAQADVEVLFKVRDLEKADVIEPSW--TDPQLICSKMNVSVKSGLGPFGLMV 468

Query: 494 LASNDLSEHTAIYFHILKAH---NRYVVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCYKK 553
           LAS +L E+T++YF I KA    N+YVVLMCSDQSRSS +++ DKT +GAFVDI+P ++ 
Sbjct: 469 LASKNLEEYTSVYFRIFKARQNSNKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQP 528

Query: 554 ISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNNARLYAFNNGTQTVVISSLKAWNMS 607
           +SLR L+DHS+VESFGGKG+ CITSRVYP LA+  ++ L+AFN G Q+V + +L AW+M+
Sbjct: 529 LSLRALIDHSVVESFGGKGRACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMN 583

BLAST of CsaV3_2G025540 vs. ExPASy Swiss-Prot
Match: Q70XE6 (Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 9.4e-171
Identity = 289/539 (53.62%), Postives = 384/539 (71.24%), Query Frame = 0

Query: 77  RTSYHFQPLKNWMNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLN 136
           RT+YHFQ  KNWMNDPNGPM YKGIYH FYQ+ P   V+++++VW HS S DLINW    
Sbjct: 56  RTAYHFQSPKNWMNDPNGPMIYKGIYHLFYQYYPYDPVWHTEIVWGHSTSTDLINWTQQP 115

Query: 137 HALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRK 196
            AL P++P+DINGCWSGS++ LP+NKP ILYTGI++ + QVQNLA+P N SDP+L++W K
Sbjct: 116 IALSPSEPYDINGCWSGSITILPQNKPVILYTGINNKNYQVQNLALPKNLSDPYLKEWIK 175

Query: 197 FSQNPIIAPP----DGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVR 256
             QNP++A      + +  + FRDP+TAWQ  DG+WRV +G Q    G A+L+ S+DFV+
Sbjct: 176 LPQNPLMAGTPTNNNNINASSFRDPSTAWQLSDGKWRVIVGTQQGKRGLAVLFTSDDFVK 235

Query: 257 WHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKYVMKASF--NSSDHYT 316
           W+    PL+S++ +G WECPDF+PV +  + G   +S  G  VK+V+K S      ++YT
Sbjct: 236 WNNTGNPLHSTEGNGIWECPDFFPVYVGKSLGAD-TSIIGDDVKHVLKLSLFDTQYEYYT 295

Query: 317 LGSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDS 376
           +G Y  EK+ +  D G        +LGLRYDYGKFYASK+F+D    RRILWGWVNES  
Sbjct: 296 IGRYDIEKDIYVPDEGS----IESDLGLRYDYGKFYASKSFFDDETNRRILWGWVNESSI 355

Query: 377 RQDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVL 436
           + DDI KGWSG+QAIPR + L K+G+QL+QWP+ E+ MLR N   L  + ++G S +E+ 
Sbjct: 356 QADDIKKGWSGVQAIPRTVVLDKSGKQLVQWPLAEVDMLRENDVELPSQVIKGGSLVEIS 415

Query: 437 GGSASQVDVEVSFELP---YLEEAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLA 496
             +ASQ DVE+SF++P   Y+EE +S  T     +PQ+LCS++ AS+ GR GPFGLL LA
Sbjct: 416 QITASQADVEISFKIPESNYVEELDSTCT-----NPQILCSQKGASIKGRFGPFGLLTLA 475

Query: 497 SNDLSEHTAIYFHILKAHNRYVVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRT 556
           S  L E+TA++F I K  N+YVVLMCSDQ+RSS     DK + G FVD+DP  + +SLR 
Sbjct: 476 SMGLEEYTAVFFRIFKGPNKYVVLMCSDQTRSSLNPTTDKLSFGIFVDVDPINEDLSLRI 535

Query: 557 LVDHSIVESFGGKGKTCITSRVYPTLAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEI 607
           L+DHSIVESF  KGK+CIT+RVYPT+A+N+ A+LY FNNGT+ V I+ L AW+M  A+I
Sbjct: 536 LIDHSIVESFSAKGKSCITARVYPTMAINDKAKLYVFNNGTEDVKITKLSAWSMKKAQI 584

BLAST of CsaV3_2G025540 vs. ExPASy Swiss-Prot
Match: Q43089 (Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 PE=2 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 9.4e-163
Identity = 275/525 (52.38%), Postives = 370/525 (70.48%), Query Frame = 0

Query: 63  SHEYANEVHTPQVQRTSYHFQPLKNWMNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWA 122
           SH  +   H P   RT+YHFQPLKNW+NDPNGPM Y G YH FYQ+NP GAV+ + +VWA
Sbjct: 35  SHHSSKSNHQP--YRTAYHFQPLKNWINDPNGPMRYGGFYHLFYQYNPKGAVWGN-IVWA 94

Query: 123 HSISYDLINWVHLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNLAI 182
           HS+S DL+NW  L+HA+ P+ P DI GCWSGS + LP  KP ILYTGID ++ QVQN+AI
Sbjct: 95  HSVSKDLVNWTPLDHAIHPSQPSDIKGCWSGSATILPGGKPAILYTGIDPNNHQVQNIAI 154

Query: 183 PANYSDPFLEKWRKFSQNPIIAP--PDGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGG 242
           P N SDP L +W+K  +NP++ P   + +  + FRDPTT+W G DG WRV IG +    G
Sbjct: 155 PKNMSDPLLREWKKSPKNPLMEPTIANKINSSSFRDPTTSWLGKDGFWRVLIGSKIDTKG 214

Query: 243 AAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNG--TNGIGFSSGFGVGVKYV 302
            A+LY+S++FV W + + PL+S++ +G WECPDFYPV+       G+  S      V++V
Sbjct: 215 MAILYKSKNFVDWVEAKHPLHSAEGTGMWECPDFYPVLDKNLLRTGVDTSRNGDDDVRHV 274

Query: 303 MKASFNSS--DHYTLGSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQ 362
           +K S + +  DHY +GSY   K+ F  + G    F+     LRYDYGK+YASKTF+D  +
Sbjct: 275 LKVSLDDTKHDHYLIGSYDVVKDVFVPENG----FEDNGFVLRYDYGKYYASKTFFDDGK 334

Query: 363 KRRILWGWVNESDSRQDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSL 422
            RRIL GWVNES S  DD+ KGWSG+  IPR+IWL ++G+QL+QWPVKEI+ LR N  + 
Sbjct: 335 NRRILLGWVNESSSVADDVKKGWSGIHTIPREIWLHESGKQLVQWPVKEIENLRMNPVNW 394

Query: 423 HHKELRGRSTMEVLGGSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNG 482
             K ++G   + + G  + Q DVE+SFE+  L + ES+  +W  +DPQ+LCS++ A V G
Sbjct: 395 PTKVIKGGERISITGVDSVQADVEISFEISDLGKVESL-RKW--IDPQLLCSQKGAGVKG 454

Query: 483 RVGPFGLLVLASNDLSEHTAIYFHILKAHNRYVVLMCSDQSRSSFRKEVDKTAHGAFVDI 542
            VGPFGLLV AS  L E+TA++F I K  ++ +VLMCSDQSRSS  K+ D T++G FVD+
Sbjct: 455 GVGPFGLLVFASQGLKEYTAVFFRIFKYQDKNLVLMCSDQSRSSLNKDNDMTSYGTFVDV 514

Query: 543 DPCYKKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNNARL 582
           DP ++K+SLRTL+DHS+VESFGG+G+ C+T+RVYPTLA+++ A L
Sbjct: 515 DPLHEKLSLRTLIDHSVVESFGGEGRACVTARVYPTLAIHDKAML 549

BLAST of CsaV3_2G025540 vs. ExPASy Swiss-Prot
Match: Q9LIB9 (Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3702 GN=CWINV5 PE=2 SV=2)

HSP 1 Score: 573.2 bits (1476), Expect = 3.6e-162
Identity = 288/541 (53.23%), Postives = 370/541 (68.39%), Query Frame = 0

Query: 74  QVQRTSYHFQPLKNWMNDPNGPMFYKGIYHFFYQHNPNGAVFN-SKMVWAHSISYDLINW 133
           Q  RT YHFQP KNWMNDPNGPM YKGIYH FYQ N NGAV + +K VW H+ S DLINW
Sbjct: 40  QPYRTGYHFQPPKNWMNDPNGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHATSTDLINW 99

Query: 134 VHLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLE 193
           + L+ A+ P+ P DINGCWSGSV+ LP  KP ILYTG D  ++QVQNL  P N +DP+L 
Sbjct: 100 ITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKNLTDPYLR 159

Query: 194 KWRKFSQNPII--APPDGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDF 253
            W K  +NP++  +P + +  + FRDPTTAW G DG WR+  G Q    G A+L+ S+DF
Sbjct: 160 HWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHTSKDF 219

Query: 254 VRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNS--SDH 313
           V W Q   PL+    +G WECPDF+PV    + G+  S   G  VK+V+K S     +D+
Sbjct: 220 VIWKQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSLTDTFNDY 279

Query: 314 YTLGSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNES 373
           YT+G+Y   ++ +  D G    F       RYDYGKFYASKTFYDS  +RRILWGWVNES
Sbjct: 280 YTIGTYDEVRDVYVPDKG----FVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWVNES 339

Query: 374 DSRQDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTME 433
              +D+I KGW+GLQAIPRK+WL ++G++L+QWPVKEI+ LR       +K L+G S ME
Sbjct: 340 SPEKDNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGSVME 399

Query: 434 VLGGSASQVDVEVSFELP--YLEEAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVL 493
           V G +A Q DVEV F++    LE+A+ ++  W   DPQ++CS+R+AS    +GPFGL+VL
Sbjct: 400 VHGVTAPQADVEVFFKVSGFDLEKADVIEPGW--TDPQLICSQRNAS--SGLGPFGLMVL 459

Query: 494 ASNDLSEHTAIYFHILKA---HNRYVVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCYKKI 553
           AS ++ E+T++   I +A      +VV+MCSDQS SS  K  DKT +GAF+DI P Y+ I
Sbjct: 460 ASKNMEEYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISP-YQPI 519

Query: 554 SLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNNARLYAFNNGTQTVVISSLKAWNMSN 605
           SLRTL+D SIVESFGGKGKTCITSRVYP LA+     L+AFN G+Q V + SL AW+M +
Sbjct: 520 SLRTLIDKSIVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSAWSMKS 571

BLAST of CsaV3_2G025540 vs. ExPASy TrEMBL
Match: A0A0A0LQX1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G351670 PE=3 SV=1)

HSP 1 Score: 1251.5 bits (3237), Expect = 0.0e+00
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 12  MEARIVCMFFEMNTTNLLFDKMPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVH 71
           MEARIVCMFFEMNTTNLLFDKMPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVH
Sbjct: 1   MEARIVCMFFEMNTTNLLFDKMPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVH 60

Query: 72  TPQVQRTSYHFQPLKNWMNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLIN 131
           TPQVQRTSYHFQPLKNWMNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLIN
Sbjct: 61  TPQVQRTSYHFQPLKNWMNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLIN 120

Query: 132 WVHLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFL 191
           WVHLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFL
Sbjct: 121 WVHLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFL 180

Query: 192 EKWRKFSQNPIIAPPDGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFV 251
           EKWRKFSQNPIIAPPDGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFV
Sbjct: 181 EKWRKFSQNPIIAPPDGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFV 240

Query: 252 RWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTL 311
           RWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTL
Sbjct: 241 RWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTL 300

Query: 312 GSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSR 371
           GSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSR
Sbjct: 301 GSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSR 360

Query: 372 QDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLG 431
           QDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLG
Sbjct: 361 QDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLG 420

Query: 432 GSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDL 491
           GSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDL
Sbjct: 421 GSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDL 480

Query: 492 SEHTAIYFHILKAHNRYVVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDH 551
           SEHTAIYFHILKAHNRYVVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDH
Sbjct: 481 SEHTAIYFHILKAHNRYVVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDH 540

Query: 552 SIVESFGGKGKTCITSRVYPTLAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEIK 608
           SIVESFGGKGKTCITSRVYPTLAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEIK
Sbjct: 541 SIVESFGGKGKTCITSRVYPTLAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEIK 596

BLAST of CsaV3_2G025540 vs. ExPASy TrEMBL
Match: A0A2I4JRG1 (Cell wall invertase 4 OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 1209.1 bits (3127), Expect = 0.0e+00
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 0

Query: 33  MPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVHTPQVQRTSYHFQPLKNWMNDP 92
           MPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVHTPQVQRTSYHFQPLKNWMNDP
Sbjct: 1   MPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVHTPQVQRTSYHFQPLKNWMNDP 60

Query: 93  NGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDINGCWS 152
           NGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDINGCWS
Sbjct: 61  NGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDINGCWS 120

Query: 153 GSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIAPPDGLERN 212
           GSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIAPPDGLERN
Sbjct: 121 GSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIAPPDGLERN 180

Query: 213 RFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECP 272
           RFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECP
Sbjct: 181 RFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECP 240

Query: 273 DFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLGSYVPEKEKFTSDYGPGFDFK 332
           DFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLGSYVPEKEKFTSDYGPGFDFK
Sbjct: 241 DFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLGSYVPEKEKFTSDYGPGFDFK 300

Query: 333 GINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRKIWLS 392
           GINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRKIWLS
Sbjct: 301 GINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRKIWLS 360

Query: 393 KTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPYLEEAE 452
           KTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPYLEEAE
Sbjct: 361 KTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPYLEEAE 420

Query: 453 SVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHNRYVVLM 512
           SVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHNRYVVLM
Sbjct: 421 SVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHNRYVVLM 480

Query: 513 CSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRVYPT 572
           CSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRVYPT
Sbjct: 481 CSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRVYPT 540

Query: 573 LAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEIK 608
           LAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEIK
Sbjct: 541 LAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEIK 575

BLAST of CsaV3_2G025540 vs. ExPASy TrEMBL
Match: A0A1S3BB78 (LOW QUALITY PROTEIN: beta-fructofuranosidase, insoluble isoenzyme CWINV3-like OS=Cucumis melo OX=3656 GN=LOC103487780 PE=3 SV=1)

HSP 1 Score: 1149.0 bits (2971), Expect = 0.0e+00
Identity = 548/579 (94.65%), Postives = 562/579 (97.06%), Query Frame = 0

Query: 31  DKMPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVH-TP-QVQRTSYHFQPLKNW 90
           DKMPPKRLE+GKYGLV SF++LL  NR+GFVVSHEYANEVH TP QVQRTSYHFQPLKNW
Sbjct: 2   DKMPPKRLEMGKYGLVASFILLLASNRMGFVVSHEYANEVHSTPQQVQRTSYHFQPLKNW 61

Query: 91  MNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDIN 150
           MNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDIN
Sbjct: 62  MNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDIN 121

Query: 151 GCWSGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIAPPDG 210
           GCWSGSVSFLP+NKPRILYTGIDSS QQVQNLAIP NYSDPFLEKWRK  QNPIIAPPDG
Sbjct: 122 GCWSGSVSFLPKNKPRILYTGIDSSFQQVQNLAIPTNYSDPFLEKWRKSPQNPIIAPPDG 181

Query: 211 LERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGT 270
            ERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGT
Sbjct: 182 AERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGT 241

Query: 271 WECPDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLGSYVPEKEKFTSDYGPG 330
           WECPDFYPVMLNGTNGIGFSSGFGVGVK+VMKASFNS DHYTLGSYVPEKEKFTSD+G G
Sbjct: 242 WECPDFYPVMLNGTNGIGFSSGFGVGVKHVMKASFNSRDHYTLGSYVPEKEKFTSDHGLG 301

Query: 331 FDFKGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRK 390
           FDF+GINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRK
Sbjct: 302 FDFRGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRK 361

Query: 391 IWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPYL 450
           IWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSF LP+L
Sbjct: 362 IWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFALPHL 421

Query: 451 EEAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHNRY 510
           EEAESVDTRWGVLDPQVLCSKRDAS+NGRVGPFGLLVLASNDLSEHTAIYFH+LKAHNRY
Sbjct: 422 EEAESVDTRWGVLDPQVLCSKRDASMNGRVGPFGLLVLASNDLSEHTAIYFHVLKAHNRY 481

Query: 511 VVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSR 570
           VVLMCSD+SRSSFRK VDKT +GAFVDIDP YKKISLRTLVDHSIVESFGGKGKTCITSR
Sbjct: 482 VVLMCSDESRSSFRKGVDKTTYGAFVDIDPRYKKISLRTLVDHSIVESFGGKGKTCITSR 541

Query: 571 VYPTLAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEIK 608
           VYPTLAVNN+ARLYAFNNGTQTVVISSLK WNMSNAEIK
Sbjct: 542 VYPTLAVNNDARLYAFNNGTQTVVISSLKVWNMSNAEIK 580

BLAST of CsaV3_2G025540 vs. ExPASy TrEMBL
Match: A0A5A7V5D6 (Beta-fructofuranosidase, insoluble isoenzyme CWINV3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001360 PE=3 SV=1)

HSP 1 Score: 983.8 bits (2542), Expect = 3.2e-283
Identity = 468/489 (95.71%), Postives = 477/489 (97.55%), Query Frame = 0

Query: 119 MVWAHSISYDLINWVHLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQ 178
           MVWAHSISYDLINWVHLNHALEPTDPFDINGCWSGSVSFLP+NKPRILYTGIDSS QQVQ
Sbjct: 1   MVWAHSISYDLINWVHLNHALEPTDPFDINGCWSGSVSFLPKNKPRILYTGIDSSFQQVQ 60

Query: 179 NLAIPANYSDPFLEKWRKFSQNPIIAPPDGLERNRFRDPTTAWQGPDGEWRVAIGGQTSY 238
           NLAIP NYSDPFLEKWRKF QNPIIAPPDG ERNRFRDPTTAWQGPDGEWRVAIGGQTSY
Sbjct: 61  NLAIPTNYSDPFLEKWRKFPQNPIIAPPDGAERNRFRDPTTAWQGPDGEWRVAIGGQTSY 120

Query: 239 GGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKYV 298
           GGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVK+V
Sbjct: 121 GGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKHV 180

Query: 299 MKASFNSSDHYTLGSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQKR 358
           MKASFNS DHYTLGSYVPEKEKFTSDYG GFDF+GINLGLRYDYGKFYASKTFYDSSQKR
Sbjct: 181 MKASFNSRDHYTLGSYVPEKEKFTSDYGLGFDFRGINLGLRYDYGKFYASKTFYDSSQKR 240

Query: 359 RILWGWVNESDSRQDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHH 418
           RILWGWVNESDSRQDDINKGWSGLQ IPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHH
Sbjct: 241 RILWGWVNESDSRQDDINKGWSGLQVIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHH 300

Query: 419 KELRGRSTMEVLGGSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNGRV 478
           KELRGRSTMEVLGGSASQVDVEVSF LP+LEEAESVDTRWGVLDPQVLCSKRDAS+NGRV
Sbjct: 301 KELRGRSTMEVLGGSASQVDVEVSFALPHLEEAESVDTRWGVLDPQVLCSKRDASMNGRV 360

Query: 479 GPFGLLVLASNDLSEHTAIYFHILKAHNRYVVLMCSDQSRSSFRKEVDKTAHGAFVDIDP 538
           GPFGLLVLASNDLSEHTAIYFH+LKAHNRYVVLMCSD+SRSSFRK VDKT +GAFVDIDP
Sbjct: 361 GPFGLLVLASNDLSEHTAIYFHVLKAHNRYVVLMCSDESRSSFRKGVDKTTYGAFVDIDP 420

Query: 539 CYKKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNNARLYAFNNGTQTVVISSLKA 598
            YKKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNN+ARLYAFNNGTQTVVISSLK 
Sbjct: 421 RYKKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNDARLYAFNNGTQTVVISSLKV 480

Query: 599 WNMSNAEIK 608
           WNMSNAEIK
Sbjct: 481 WNMSNAEIK 489

BLAST of CsaV3_2G025540 vs. ExPASy TrEMBL
Match: A0A6J1HDH1 (beta-fructofuranosidase, insoluble isoenzyme CWINV3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462607 PE=3 SV=1)

HSP 1 Score: 976.9 bits (2524), Expect = 4.0e-281
Identity = 466/576 (80.90%), Postives = 510/576 (88.54%), Query Frame = 0

Query: 33  MPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVHTP-QVQRTSYHFQPLKNWMND 92
           M  K +E+ K GLV  + + L  NR GFVVSH   NEVH P Q QRTSYHFQPL+NWMND
Sbjct: 1   MATKWMEMVKCGLVALYFVWLISNRFGFVVSHGDINEVHIPQQFQRTSYHFQPLQNWMND 60

Query: 93  PNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDINGCW 152
           PNGPM+YKGIYH FYQHNPNGAVF+SK+VW H++S DLINW HLNHALEPT PFDINGCW
Sbjct: 61  PNGPMYYKGIYHLFYQHNPNGAVFDSKIVWGHAVSRDLINWAHLNHALEPTHPFDINGCW 120

Query: 153 SGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIAPPDGLER 212
           SGSVSFL  NKP ILYTG+DSS QQVQNLAIP+N+SDPFLE W K S NPIIAPP+G+ER
Sbjct: 121 SGSVSFLHNNKPVILYTGLDSSFQQVQNLAIPSNFSDPFLEIWTKSSHNPIIAPPNGIER 180

Query: 213 NRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWEC 272
           +RFRDPTTAW GPDG WR+ IGGQTSYGGAAMLYRSEDFV WHQ RFPLYSS+DSG WEC
Sbjct: 181 HRFRDPTTAWPGPDGGWRIVIGGQTSYGGAAMLYRSEDFVHWHQSRFPLYSSRDSGNWEC 240

Query: 273 PDFYPVMLNGTNGIGFSSGFGVG-VKYVMKASFNSSDHYTLGSYVPEKEKFTSDYGPGFD 332
           PDFYPV LNG+NGI   +GF VG VKYVMKASFNS D+YTLGSYVPEKEKFTSD G GFD
Sbjct: 241 PDFYPVKLNGSNGIDSLNGFRVGAVKYVMKASFNSHDYYTLGSYVPEKEKFTSDSGDGFD 300

Query: 333 FKGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRKIW 392
            +G NLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSR+D+I KGW+GLQ +PRKIW
Sbjct: 301 LRGTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDNIKKGWAGLQVVPRKIW 360

Query: 393 LSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPYLEE 452
           LSKTGRQLIQWPVKE++ LR N FSL++KELRGRSTM+VLGGSASQVD EVSFE PYLEE
Sbjct: 361 LSKTGRQLIQWPVKEVEKLRTNRFSLYNKELRGRSTMKVLGGSASQVDAEVSFEFPYLEE 420

Query: 453 AESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHNRYVV 512
           AE VDTRW  LDPQ+LCSKRDASVNGRVGPFGLLVLASNDLSEHTAI+F +LKAHNRYVV
Sbjct: 421 AERVDTRWFDLDPQLLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIFFRVLKAHNRYVV 480

Query: 513 LMCSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRVY 572
           LMCSDQSRSSFR+ VDKT +GAFVD+DP ++KISLRTLVD SIVESFGGKGKTCITSRVY
Sbjct: 481 LMCSDQSRSSFREGVDKTTYGAFVDVDPRHEKISLRTLVDRSIVESFGGKGKTCITSRVY 540

Query: 573 PTLAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEI 607
           PTLAV+++ARLYAFNNGTQ+VVISSLK W M NA+I
Sbjct: 541 PTLAVDDDARLYAFNNGTQSVVISSLKVWKMWNAQI 576

BLAST of CsaV3_2G025540 vs. TAIR 10
Match: AT1G55120.1 (beta-fructofuranosidase 5 )

HSP 1 Score: 625.2 bits (1611), Expect = 5.6e-179
Identity = 304/571 (53.24%), Postives = 397/571 (69.53%), Query Frame = 0

Query: 47  VSFVILLTCNRIGFVVSHEYANEVHTPQVQRTSYHFQPLKNWMNDPNGPMFYKGIYHFFY 106
           +   +LL+     F+   E ++     Q  RT YHFQPLKNWMNDPNGPM YKGIYH FY
Sbjct: 9   IGLSLLLSMFLANFITDLEASSHQDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGIYHLFY 68

Query: 107 QHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDINGCWSGSVSFLPENKPRIL 166
           Q+NP GAV++ ++VW HS S DL+NW+    A  P+ P DINGCWSGSV+ LP  KP IL
Sbjct: 69  QYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSGSVTILPNGKPVIL 128

Query: 167 YTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIA--PPDGLERNRFRDPTTAWQGP 226
           YTGID +  QVQN+A+P N SDP+L +W K  QNP++     +G+  +RFRDPTTAW G 
Sbjct: 129 YTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINPDRFRDPTTAWLGR 188

Query: 227 DGEWRVAIGGQT-SYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTN 286
           DGEWRV +G  T    G A+LY+S DF  W Q   PL+    +G WECPDF+PV + G++
Sbjct: 189 DGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWECPDFFPVSITGSD 248

Query: 287 GIGFSSGFGVGVKYVMKASFNSS--DHYTLGSYVPEKEKFTSDYGPGFDFKGINLGLRYD 346
           G+  SS    G+K+V+K S   +  D+YT+GSY  EK+ +  D G    F       R D
Sbjct: 249 GVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDLG----FVQNESAPRLD 308

Query: 347 YGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRKIWLSKTGRQLIQW 406
           YGK+YASKTFYD  +KRRILWGWVNES   +DDI KGWSGLQ+ PRKIWL ++G++L+QW
Sbjct: 309 YGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLDESGKELLQW 368

Query: 407 PVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPYLEEAESVDTRWGVL 466
           P++EI+ LR    +   K L+  ST++V G +A+Q DVEVSF++  LE+A+ ++  W   
Sbjct: 369 PIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKADVIEPSW--T 428

Query: 467 DPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHN------RYVVLMCSD 526
           DPQ +CS+ D SV   +GPFGL+VLASND+ E+T++YF I K+++      +YVVLMCSD
Sbjct: 429 DPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCSD 488

Query: 527 QSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAV 586
           QSRSS   E DK+  GAFV IDP ++ ISLRTL+DHSIVES+GG G+TCITSRVYP LA+
Sbjct: 489 QSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTCITSRVYPKLAI 548

Query: 587 NNNARLYAFNNGTQTVVISSLKAWNMSNAEI 607
             NA L+ FN GTQ+V I +L AW++ +A+I
Sbjct: 549 GENANLFVFNKGTQSVDILTLSAWSLKSAQI 573

BLAST of CsaV3_2G025540 vs. TAIR 10
Match: AT3G13790.1 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 606.3 bits (1562), Expect = 2.7e-173
Identity = 299/544 (54.96%), Postives = 393/544 (72.24%), Query Frame = 0

Query: 74  QVQRTSYHFQPLKNWMNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWV 133
           Q  RT +HFQP KNWMNDPNGPM YKGIYH FYQ NP GAV+ + +VWAHS S DLINW 
Sbjct: 49  QPYRTGFHFQPPKNWMNDPNGPMIYKGIYHLFYQWNPKGAVWGN-IVWAHSTSTDLINWD 108

Query: 134 HLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEK 193
               A+ P+ PFDINGCWSGS + LP  KP ILYTGID  +QQVQN+A P N SDP+L +
Sbjct: 109 PHPPAIFPSAPFDINGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLRE 168

Query: 194 WRKFSQNPIIAPP--DGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFV 253
           W+K   NP++AP   +G+  + FRDPTTAW G D +WRV IG +    G A+ Y S+DF+
Sbjct: 169 WKKSPLNPLMAPDAVNGINASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFL 228

Query: 254 RWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVG---VKYVMKASFNSS-- 313
           +W +   PL+    SG WECPDF+PV   G+NG+  SS FG     +K+V+K S + +  
Sbjct: 229 KWEKSPEPLHYDDGSGMWECPDFFPVTRFGSNGVETSS-FGEPNEILKHVLKISLDDTKH 288

Query: 314 DHYTLGSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVN 373
           D+YT+G+Y   K+KF  D G    FK      RYDYGK+YASKTF+DS++ RRILWGW N
Sbjct: 289 DYYTIGTYDRVKDKFVPDNG----FKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTN 348

Query: 374 ESDSRQDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHF-SLHHKELRGRS 433
           ES S +DD+ KGWSG+Q IPRKIWL ++G+QLIQWPV+E++ LR     +L +K L+  S
Sbjct: 349 ESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGS 408

Query: 434 TMEVLGGSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLV 493
            +EV G +A+Q DVEV F++  LE+A+ ++  W   DPQ++CSK + SV   +GPFGL+V
Sbjct: 409 RLEVYGVTAAQADVEVLFKVRDLEKADVIEPSW--TDPQLICSKMNVSVKSGLGPFGLMV 468

Query: 494 LASNDLSEHTAIYFHILKAH---NRYVVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCYKK 553
           LAS +L E+T++YF I KA    N+YVVLMCSDQSRSS +++ DKT +GAFVDI+P ++ 
Sbjct: 469 LASKNLEEYTSVYFRIFKARQNSNKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQP 528

Query: 554 ISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNNARLYAFNNGTQTVVISSLKAWNMS 607
           +SLR L+DHS+VESFGGKG+ CITSRVYP LA+  ++ L+AFN G Q+V + +L AW+M+
Sbjct: 529 LSLRALIDHSVVESFGGKGRACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMN 583

BLAST of CsaV3_2G025540 vs. TAIR 10
Match: AT3G13790.2 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 593.6 bits (1529), Expect = 1.8e-169
Identity = 296/544 (54.41%), Postives = 390/544 (71.69%), Query Frame = 0

Query: 74  QVQRTSYHFQPLKNWMNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWV 133
           Q  RT +HFQP KNWM   NGPM YKGIYH FYQ NP GAV+ + +VWAHS S DLINW 
Sbjct: 49  QPYRTGFHFQPPKNWM---NGPMIYKGIYHLFYQWNPKGAVWGN-IVWAHSTSTDLINWD 108

Query: 134 HLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEK 193
               A+ P+ PFDINGCWSGS + LP  KP ILYTGID  +QQVQN+A P N SDP+L +
Sbjct: 109 PHPPAIFPSAPFDINGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLRE 168

Query: 194 WRKFSQNPIIAPP--DGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFV 253
           W+K   NP++AP   +G+  + FRDPTTAW G D +WRV IG +    G A+ Y S+DF+
Sbjct: 169 WKKSPLNPLMAPDAVNGINASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFL 228

Query: 254 RWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVG---VKYVMKASFNSS-- 313
           +W +   PL+    SG WECPDF+PV   G+NG+  SS FG     +K+V+K S + +  
Sbjct: 229 KWEKSPEPLHYDDGSGMWECPDFFPVTRFGSNGVETSS-FGEPNEILKHVLKISLDDTKH 288

Query: 314 DHYTLGSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVN 373
           D+YT+G+Y   K+KF  D G    FK      RYDYGK+YASKTF+DS++ RRILWGW N
Sbjct: 289 DYYTIGTYDRVKDKFVPDNG----FKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTN 348

Query: 374 ESDSRQDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHF-SLHHKELRGRS 433
           ES S +DD+ KGWSG+Q IPRKIWL ++G+QLIQWPV+E++ LR     +L +K L+  S
Sbjct: 349 ESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGS 408

Query: 434 TMEVLGGSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLV 493
            +EV G +A+Q DVEV F++  LE+A+ ++  W   DPQ++CSK + SV   +GPFGL+V
Sbjct: 409 RLEVYGVTAAQADVEVLFKVRDLEKADVIEPSW--TDPQLICSKMNVSVKSGLGPFGLMV 468

Query: 494 LASNDLSEHTAIYFHILKAH---NRYVVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCYKK 553
           LAS +L E+T++YF I KA    N+YVVLMCSDQSRSS +++ DKT +GAFVDI+P ++ 
Sbjct: 469 LASKNLEEYTSVYFRIFKARQNSNKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQP 528

Query: 554 ISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNNARLYAFNNGTQTVVISSLKAWNMS 607
           +SLR L+DHS+VESFGGKG+ CITSRVYP LA+  ++ L+AFN G Q+V + +L AW+M+
Sbjct: 529 LSLRALIDHSVVESFGGKGRACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMN 580

BLAST of CsaV3_2G025540 vs. TAIR 10
Match: AT1G55120.3 (beta-fructofuranosidase 5 )

HSP 1 Score: 583.2 bits (1502), Expect = 2.5e-166
Identity = 283/525 (53.90%), Postives = 371/525 (70.67%), Query Frame = 0

Query: 93  NGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDINGCWS 152
           +GPM YKGIYH FYQ+NP GAV++ ++VW HS S DL+NW+    A  P+ P DINGCWS
Sbjct: 40  SGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWS 99

Query: 153 GSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIA--PPDGLE 212
           GSV+ LP  KP ILYTGID +  QVQN+A+P N SDP+L +W K  QNP++     +G+ 
Sbjct: 100 GSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGIN 159

Query: 213 RNRFRDPTTAWQGPDGEWRVAIGGQT-SYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTW 272
            +RFRDPTTAW G DGEWRV +G  T    G A+LY+S DF  W Q   PL+    +G W
Sbjct: 160 PDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMW 219

Query: 273 ECPDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSS--DHYTLGSYVPEKEKFTSDYGP 332
           ECPDF+PV + G++G+  SS    G+K+V+K S   +  D+YT+GSY  EK+ +  D G 
Sbjct: 220 ECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDLG- 279

Query: 333 GFDFKGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPR 392
              F       R DYGK+YASKTFYD  +KRRILWGWVNES   +DDI KGWSGLQ+ PR
Sbjct: 280 ---FVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPR 339

Query: 393 KIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPY 452
           KIWL ++G++L+QWP++EI+ LR    +   K L+  ST++V G +A+Q DVEVSF++  
Sbjct: 340 KIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKE 399

Query: 453 LEEAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHN- 512
           LE+A+ ++  W   DPQ +CS+ D SV   +GPFGL+VLASND+ E+T++YF I K+++ 
Sbjct: 400 LEKADVIEPSW--TDPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDD 459

Query: 513 -----RYVVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKG 572
                +YVVLMCSDQSRSS   E DK+  GAFV IDP ++ ISLRTL+DHSIVES+GG G
Sbjct: 460 TNKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGG 519

Query: 573 KTCITSRVYPTLAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEI 607
           +TCITSRVYP LA+  NA L+ FN GTQ+V I +L AW++ +A+I
Sbjct: 520 RTCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQI 558

BLAST of CsaV3_2G025540 vs. TAIR 10
Match: AT1G55120.2 (beta-fructofuranosidase 5 )

HSP 1 Score: 582.8 bits (1501), Expect = 3.2e-166
Identity = 283/524 (54.01%), Postives = 370/524 (70.61%), Query Frame = 0

Query: 94  GPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDINGCWSG 153
           GPM YKGIYH FYQ+NP GAV++ ++VW HS S DL+NW+    A  P+ P DINGCWSG
Sbjct: 17  GPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSG 76

Query: 154 SVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIA--PPDGLER 213
           SV+ LP  KP ILYTGID +  QVQN+A+P N SDP+L +W K  QNP++     +G+  
Sbjct: 77  SVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINP 136

Query: 214 NRFRDPTTAWQGPDGEWRVAIGGQT-SYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWE 273
           +RFRDPTTAW G DGEWRV +G  T    G A+LY+S DF  W Q   PL+    +G WE
Sbjct: 137 DRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWE 196

Query: 274 CPDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSS--DHYTLGSYVPEKEKFTSDYGPG 333
           CPDF+PV + G++G+  SS    G+K+V+K S   +  D+YT+GSY  EK+ +  D G  
Sbjct: 197 CPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDLG-- 256

Query: 334 FDFKGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRK 393
             F       R DYGK+YASKTFYD  +KRRILWGWVNES   +DDI KGWSGLQ+ PRK
Sbjct: 257 --FVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRK 316

Query: 394 IWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPYL 453
           IWL ++G++L+QWP++EI+ LR    +   K L+  ST++V G +A+Q DVEVSF++  L
Sbjct: 317 IWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKEL 376

Query: 454 EEAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHN-- 513
           E+A+ ++  W   DPQ +CS+ D SV   +GPFGL+VLASND+ E+T++YF I K+++  
Sbjct: 377 EKADVIEPSW--TDPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDT 436

Query: 514 ----RYVVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGK 573
               +YVVLMCSDQSRSS   E DK+  GAFV IDP ++ ISLRTL+DHSIVES+GG G+
Sbjct: 437 NKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGR 496

Query: 574 TCITSRVYPTLAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEI 607
           TCITSRVYP LA+  NA L+ FN GTQ+V I +L AW++ +A+I
Sbjct: 497 TCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQI 534

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004142928.30.0e+00100.00beta-fructofuranosidase, insoluble isoenzyme CWINV3 isoform X1 [Cucumis sativus]... [more]
XP_011649529.20.0e+0099.51beta-fructofuranosidase, insoluble isoenzyme CWINV3 isoform X2 [Cucumis sativus][more]
ALS30215.10.0e+00100.00cell wall invertase 4 [Cucumis sativus][more]
XP_008444460.10.0e+0094.65PREDICTED: LOW QUALITY PROTEIN: beta-fructofuranosidase, insoluble isoenzyme CWI... [more]
KAA0061001.16.7e-28395.71beta-fructofuranosidase, insoluble isoenzyme CWINV3-like [Cucumis melo var. maku... [more]
Match NameE-valueIdentityDescription
Q67XZ37.9e-17853.24Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3... [more]
Q438663.8e-17254.96Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3... [more]
Q70XE69.4e-17153.62Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1[more]
Q430899.4e-16352.38Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 P... [more]
Q9LIB93.6e-16253.23Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A0A0LQX10.0e+00100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G351670 PE=3 SV=1[more]
A0A2I4JRG10.0e+00100.00Cell wall invertase 4 OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
A0A1S3BB780.0e+0094.65LOW QUALITY PROTEIN: beta-fructofuranosidase, insoluble isoenzyme CWINV3-like OS... [more]
A0A5A7V5D63.2e-28395.71Beta-fructofuranosidase, insoluble isoenzyme CWINV3-like OS=Cucumis melo var. ma... [more]
A0A6J1HDH14.0e-28180.90beta-fructofuranosidase, insoluble isoenzyme CWINV3-like isoform X1 OS=Cucurbita... [more]
Match NameE-valueIdentityDescription
AT1G55120.15.6e-17953.24beta-fructofuranosidase 5 [more]
AT3G13790.12.7e-17354.96Glycosyl hydrolases family 32 protein [more]
AT3G13790.21.8e-16954.41Glycosyl hydrolases family 32 protein [more]
AT1G55120.32.5e-16653.90beta-fructofuranosidase 5 [more]
AT1G55120.23.2e-16654.01beta-fructofuranosidase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001362Glycoside hydrolase, family 32SMARTSM00640glyco_32coord: 81..561
e-value: 1.7E-211
score: 718.5
IPR013189Glycosyl hydrolase family 32, C-terminalPFAMPF08244Glyco_hydro_32Ccoord: 405..600
e-value: 1.0E-32
score: 113.3
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilyGENE3D2.115.10.20Glycosyl hydrolase domain; family 43coord: 85..414
e-value: 9.0E-108
score: 362.5
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilySUPERFAMILY75005Arabinanase/levansucrase/invertasecoord: 73..403
IPR013148Glycosyl hydrolase family 32, N-terminalPFAMPF00251Glyco_hydro_32Ncoord: 81..402
e-value: 1.6E-95
score: 320.2
NoneNo IPR availableGENE3D2.60.120.560coord: 415..601
e-value: 5.0E-75
score: 253.3
NoneNo IPR availablePANTHERPTHR31953:SF66BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV6coord: 69..606
NoneNo IPR availablePANTHERPTHR31953BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATEDcoord: 69..606
NoneNo IPR availableCDDcd18624GH32_Fruct1-likecoord: 87..391
e-value: 5.45367E-150
score: 432.581
IPR018053Glycoside hydrolase, family 32, active sitePROSITEPS00609GLYCOSYL_HYDROL_F32coord: 81..94
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 402..603

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_2G025540.1CsaV3_2G025540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds