CsaV3_2G017610 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_2G017610
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	Pectinesterase
Location	chr2: 14628980 .. 14631634 (-)
RNA-Seq Expression	CsaV3_2G017610
Synteny	CsaV3_2G017610

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGCCTAGAAAGCATACATACACCATGGTTTCTTCAAACCCCATCTCTCTTCTCACATTTCTCACTCTCCTCTTCCTCCTCTCTCCCTCGTCCTCGGCCGCCCCAACGGCGGACATTTGCCAATCCACTACCTCCCCACCCTTCTGCAACTCCTTCCTTCCTAAGTCTCCAGACTCCATCCACTCTCATTGTCGCTTCACTCTTCACCAAGCCCTCGCCCATGCACGAACCTTCCTGTCACTCGTCAACGCCCAACTCAACCTCTTACCCTCCCTCTCTGCCCTCCACGATTGTCGTTGCCTGGCGGAAGCCAACCTCGACTTCTTATTCCAAACTTTCTCCATAGTCAACTCCACTACCACGACGCTTCCCTATTACGATGCCCACGAGATGTTATCTTTGATAAGCGCAATTATCACTAACGTAGACACTTGTTATGAAGGGTTGGCGAGTTTAAACTCAGCCGTGGGCTTGGTTGATAAGGTGTTGGAAGCTATCTCTTTTGATAAGAAGCTATATAGTCTGTACCTGTCACTGTTCAAAATGGGTTGGGTTTCTAAGGACTTGAAGGCTCCTACGTTCCCAAAAATGAACCATTTTGGGGCTGGGAAAGGGCAGTTGAAGCTTAAAATGTCGCCCAAAGACCGTGCTTATTATGAGCGCTTGGTCCATAGGAACAAGCCGCCGGGTGCTAGAAGGCTTCTTCAAACCAATTACCAAGATGATGGAATTGTGGTCAATGGTAATAAACCCTTCATTATTCCATTTCAAATATTTTTACTAAATTACAAAACTACCCTTATTTACAAAATCTATCATTAATTTTTTGAAATAATTAATATATCACATAGAATTTTGAAAAAATATTTTTTCCATCACTCCTTTTAGTCTTCCTTTGGACTCTATTTTTCAAAATCATATGCATTTAGTTTTTTCTCTATCTTTCAAAATTTACACTTTAAAAATTGTTTTTTTTCTTTAAATCGACTAATATTAATGTCTATTAATTAATATAAAATTATTTTAATTTTTAATTTATTTTGAAAGGTATTGTAATAGAACTCGAACCACCTCTTATGTTTGAGTACAGGTAGATGTTAGTTGAGTTGAAATCACTTTTAGTAAAATTTGAATTTTCAACCGTGATGAATTAATAATTTTAATTTTTTTCAATTAATTAATAGACATTACCGCTAAAGATGACAAGTCCATATTTAGTGGGAAAAAATGGGGACTAAAAATGTAAATTTGAAACCTTAAAATTAAATTTGCACAAAATAAGAATAAAATTATATAAGATTTTGAAATGAATGGACTAATTAGTTAAATTGAAACTAATCTTCAAACTTGAAAACTAAATAAAAACTATATAGAATCAAAATATATTTTTCCCTCAAGACTACTTTTGGAACATGGATTTCTTCTATATTAATTATTTTGTTCAATAGGCATTGTTGGTGTGGATCAAAATGGAATGTACGACTTCACAAATATCACAGCCGCCATTGCTGCAGCTCCCAACAAGACCACCGTCGCTAAGGGATACTTCTTGATCTTCGTCGCCGCTGGCATTTACAACGAGACCGTTTTGGTTCCTAAAGAAAAAAGATACGTTCTGTTGATCGGAGAAGGTAACAACCAAACCATCATCACCGGCAACAAAAACGTCGTTGATGGCTCAACCACTTTCAATAGCGCAACAGTTGGTGAGCTCCTCAAAAACCCTACTCATAACTATAATTTAGGGTGTTTTCCAATACAAAAAAAGACGTTAATGTCACTGTTAATATATATATATATATATATATATATATATATCAGTTACATGTGTTAATATATATCAATTAACAAATCTAATGATACACAAATCACTATTATCTGCAGCTGTGGAGGGAACGGGGTTCTTGGGGGTGAACCTAACGATCACCAACACAGCTGGATCTGCCAAACATCAAGCAGTTGCGCTGCGAGTGAGCGCCGACAACGTGACGTTATACAACTGCATATTCGAAGGCTATCAAGACACCCTCTACACGCACTCCCTCCGTCAATTCTATAGAGAATGTGACGTCTACGGTACCGTCGATTTCATCTTCGGTAACGCTGCCGTCGTTTTACAAAACTGTAACATCTACGCCCGCCTCCCGATGAGTGGCCAGTTCAACGCCCTCACAGCGCAAGGCCGAACCGACCCTAATCAAAACACGGGAACCTCGATCCACAATTGCACCATCAAGGCGACGCCAGAGCTCGCGGCAAGCCCGGCCACGAAGAGCTACTTAGGGCGGCCGTGGAAGCAATATTCTCGAACAGTTTATATGCAATCATTTATTGATTCTTTTATTGATCCTGTAGGGTGGAAGGAGTGGGACGGCACTTTGAATTTGAACACTTCTTATTATGCTGAGTTTAATAACTCCGGGCCGGGCTGCGACACTTCACAGCGGGCTTCGTGGGCGGTTGGTGTCATTAACGCCACCGTTGCTAGTAACTTCACGGTGTCTCAATTATTGGCCGGAGATAAATGGCTGCCGCCCACCGAGGTGCCTTACACCGGTGGTTTGATATCATAAACAACTAAACCAGATTTAATCAATCGATTTCCTTATGATTGGATTTTAATTTGATGTATTACTGAATTTTGTTTTAAAGGTG

mRNA sequence

ATGGTTTCTTCAAACCCCATCTCTCTTCTCACATTTCTCACTCTCCTCTTCCTCCTCTCTCCCTCGTCCTCGGCCGCCCCAACGGCGGACATTTGCCAATCCACTACCTCCCCACCCTTCTGCAACTCCTTCCTTCCTAAGTCTCCAGACTCCATCCACTCTCATTGTCGCTTCACTCTTCACCAAGCCCTCGCCCATGCACGAACCTTCCTGTCACTCGTCAACGCCCAACTCAACCTCTTACCCTCCCTCTCTGCCCTCCACGATTGTCGTTGCCTGGCGGAAGCCAACCTCGACTTCTTATTCCAAACTTTCTCCATAGTCAACTCCACTACCACGACGCTTCCCTATTACGATGCCCACGAGATGTTATCTTTGATAAGCGCAATTATCACTAACGTAGACACTTGTTATGAAGGGTTGGCGAGTTTAAACTCAGCCGTGGGCTTGGTTGATAAGGTGTTGGAAGCTATCTCTTTTGATAAGAAGCTATATAGTCTGTACCTGTCACTGTTCAAAATGGGTTGGGTTTCTAAGGACTTGAAGGCTCCTACGTTCCCAAAAATGAACCATTTTGGGGCTGGGAAAGGGCAGTTGAAGCTTAAAATGTCGCCCAAAGACCGTGCTTATTATGAGCGCTTGGTCCATAGGAACAAGCCGCCGGGTGCTAGAAGGCTTCTTCAAACCAATTACCAAGATGATGGAATTGTGGTCAATGGCATTGTTGGTGTGGATCAAAATGGAATGTACGACTTCACAAATATCACAGCCGCCATTGCTGCAGCTCCCAACAAGACCACCGTCGCTAAGGGATACTTCTTGATCTTCGTCGCCGCTGGCATTTACAACGAGACCGTTTTGGTTCCTAAAGAAAAAAGATACGTTCTGTTGATCGGAGAAGGTAACAACCAAACCATCATCACCGGCAACAAAAACGTCGTTGATGGCTCAACCACTTTCAATAGCGCAACAGTTGCTGTGGAGGGAACGGGGTTCTTGGGGGTGAACCTAACGATCACCAACACAGCTGGATCTGCCAAACATCAAGCAGTTGCGCTGCGAGTGAGCGCCGACAACGTGACGTTATACAACTGCATATTCGAAGGCTATCAAGACACCCTCTACACGCACTCCCTCCGTCAATTCTATAGAGAATGTGACGTCTACGGTACCGTCGATTTCATCTTCGGTAACGCTGCCGTCGTTTTACAAAACTGTAACATCTACGCCCGCCTCCCGATGAGTGGCCAGTTCAACGCCCTCACAGCGCAAGGCCGAACCGACCCTAATCAAAACACGGGAACCTCGATCCACAATTGCACCATCAAGGCGACGCCAGAGCTCGCGGCAAGCCCGGCCACGAAGAGCTACTTAGGGCGGCCGTGGAAGCAATATTCTCGAACAGTTTATATGCAATCATTTATTGATTCTTTTATTGATCCTGTAGGGTGGAAGGAGTGGGACGGCACTTTGAATTTGAACACTTCTTATTATGCTGAGTTTAATAACTCCGGGCCGGGCTGCGACACTTCACAGCGGGCTTCGTGGGCGGTTGGTGTCATTAACGCCACCGTTGCTAGTAACTTCACGGTGTCTCAATTATTGGCCGGAGATAAATGGCTGCCGCCCACCGAGGTGCCTTACACCGGTGGTTTGATATCATAA

Coding sequence (CDS)

ATGGTTTCTTCAAACCCCATCTCTCTTCTCACATTTCTCACTCTCCTCTTCCTCCTCTCTCCCTCGTCCTCGGCCGCCCCAACGGCGGACATTTGCCAATCCACTACCTCCCCACCCTTCTGCAACTCCTTCCTTCCTAAGTCTCCAGACTCCATCCACTCTCATTGTCGCTTCACTCTTCACCAAGCCCTCGCCCATGCACGAACCTTCCTGTCACTCGTCAACGCCCAACTCAACCTCTTACCCTCCCTCTCTGCCCTCCACGATTGTCGTTGCCTGGCGGAAGCCAACCTCGACTTCTTATTCCAAACTTTCTCCATAGTCAACTCCACTACCACGACGCTTCCCTATTACGATGCCCACGAGATGTTATCTTTGATAAGCGCAATTATCACTAACGTAGACACTTGTTATGAAGGGTTGGCGAGTTTAAACTCAGCCGTGGGCTTGGTTGATAAGGTGTTGGAAGCTATCTCTTTTGATAAGAAGCTATATAGTCTGTACCTGTCACTGTTCAAAATGGGTTGGGTTTCTAAGGACTTGAAGGCTCCTACGTTCCCAAAAATGAACCATTTTGGGGCTGGGAAAGGGCAGTTGAAGCTTAAAATGTCGCCCAAAGACCGTGCTTATTATGAGCGCTTGGTCCATAGGAACAAGCCGCCGGGTGCTAGAAGGCTTCTTCAAACCAATTACCAAGATGATGGAATTGTGGTCAATGGCATTGTTGGTGTGGATCAAAATGGAATGTACGACTTCACAAATATCACAGCCGCCATTGCTGCAGCTCCCAACAAGACCACCGTCGCTAAGGGATACTTCTTGATCTTCGTCGCCGCTGGCATTTACAACGAGACCGTTTTGGTTCCTAAAGAAAAAAGATACGTTCTGTTGATCGGAGAAGGTAACAACCAAACCATCATCACCGGCAACAAAAACGTCGTTGATGGCTCAACCACTTTCAATAGCGCAACAGTTGCTGTGGAGGGAACGGGGTTCTTGGGGGTGAACCTAACGATCACCAACACAGCTGGATCTGCCAAACATCAAGCAGTTGCGCTGCGAGTGAGCGCCGACAACGTGACGTTATACAACTGCATATTCGAAGGCTATCAAGACACCCTCTACACGCACTCCCTCCGTCAATTCTATAGAGAATGTGACGTCTACGGTACCGTCGATTTCATCTTCGGTAACGCTGCCGTCGTTTTACAAAACTGTAACATCTACGCCCGCCTCCCGATGAGTGGCCAGTTCAACGCCCTCACAGCGCAAGGCCGAACCGACCCTAATCAAAACACGGGAACCTCGATCCACAATTGCACCATCAAGGCGACGCCAGAGCTCGCGGCAAGCCCGGCCACGAAGAGCTACTTAGGGCGGCCGTGGAAGCAATATTCTCGAACAGTTTATATGCAATCATTTATTGATTCTTTTATTGATCCTGTAGGGTGGAAGGAGTGGGACGGCACTTTGAATTTGAACACTTCTTATTATGCTGAGTTTAATAACTCCGGGCCGGGCTGCGACACTTCACAGCGGGCTTCGTGGGCGGTTGGTGTCATTAACGCCACCGTTGCTAGTAACTTCACGGTGTCTCAATTATTGGCCGGAGATAAATGGCTGCCGCCCACCGAGGTGCCTTACACCGGTGGTTTGATATCATAA

Protein sequence

MVSSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDLKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNGIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKWLPPTEVPYTGGLIS*

Homology

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BLAST of CsaV3_2G017610 vs. NCBI nr
Match: XP_004148470.1 (pectinesterase [Cucumis sativus] >KAE8651957.1 hypothetical protein Csa_006047 [Cucumis sativus])

HSP 1 Score: 1104.4 bits (2855), Expect = 0.0e+00
Identity = 554/554 (100.00%), Postives = 554/554 (100.00%), Query Frame = 0


Query: 1   MVSSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL 60
           MVSSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL
Sbjct: 1   MVSSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL 60

Query: 61  HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDA 120
           HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDA
Sbjct: 61  HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDA 120

Query: 121 HEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKD 180
           HEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKD
Sbjct: 121 HEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKD 180

Query: 181 LKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNG 240
           LKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNG
Sbjct: 181 LKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNG 240

Query: 241 IVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGE 300
           IVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGE
Sbjct: 241 IVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGE 300

Query: 301 GNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNV 360
           GNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNV
Sbjct: 301 GNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNV 360

Query: 361 TLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNA 420
           TLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNA
Sbjct: 361 TLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNA 420

Query: 421 LTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFID 480
           LTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFID
Sbjct: 421 LTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFID 480

Query: 481 PVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKW 540
           PVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKW
Sbjct: 481 PVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKW 540

Query: 541 LPPTEVPYTGGLIS 555
           LPPTEVPYTGGLIS
Sbjct: 541 LPPTEVPYTGGLIS 554

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BLAST of CsaV3_2G017610 vs. NCBI nr
Match: XP_008457895.1 (PREDICTED: pectinesterase-like [Cucumis melo] >KAA0045890.1 pectinesterase-like [Cucumis melo var. makuwa] >TYK13697.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1028.9 bits (2659), Expect = 1.7e-296
Identity = 511/555 (92.07%), Postives = 530/555 (95.50%), Query Frame = 0


Query: 1   MVSSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL 60
           MVSSNPIS+LTFLTLLF LSPSSSA PTA+ICQSTTSPPFCN FLP SP S+HSHCRFTL
Sbjct: 1   MVSSNPISVLTFLTLLFFLSPSSSAVPTAEICQSTTSPPFCNFFLPNSPASVHSHCRFTL 60

Query: 61  HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTT-LPYYD 120
           H AL HARTFLSLVNA LNL PSLS LHDCR LAEANLDFL QTFSIVNSTTTT LPYYD
Sbjct: 61  HHALVHARTFLSLVNAHLNLFPSLSPLHDCRSLAEANLDFLSQTFSIVNSTTTTLLPYYD 120

Query: 121 AHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK 180
           AHE L+L+SA+ITN DTCYEGLA L+SAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK
Sbjct: 121 AHEKLTLMSAVITNEDTCYEGLAGLDSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK 180

Query: 181 DLKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVN 240
           DLKAPT PKM HFG GKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIV+N
Sbjct: 181 DLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVIN 240

Query: 241 GIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIG 300
           GIVGVDQNG YDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVL+PKEKRYVLLIG
Sbjct: 241 GIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLIPKEKRYVLLIG 300

Query: 301 EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADN 360
           EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTI+NTAG+AKHQAVALRVSADN
Sbjct: 301 EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTAGAAKHQAVALRVSADN 360

Query: 361 VTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFN 420
            TLYNCIFEGYQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLP+SGQFN
Sbjct: 361 ATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFN 420

Query: 421 ALTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI 480
           A+TAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI
Sbjct: 421 AVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI 480

Query: 481 DPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDK 540
           DP GWKEWDGT+NLNTSYYAEFNNSGPG DTSQR SWAVGVINATVA++FTVSQ LAGD+
Sbjct: 481 DPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINATVANSFTVSQFLAGDR 540

Query: 541 WLPPTEVPYTGGLIS 555
           WLPPT+VPYTGGLIS
Sbjct: 541 WLPPTDVPYTGGLIS 555

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BLAST of CsaV3_2G017610 vs. NCBI nr
Match: XP_038902853.1 (probable pectinesterase/pectinesterase inhibitor 20 [Benincasa hispida])

HSP 1 Score: 951.0 bits (2457), Expect = 4.4e-273
Identity = 471/555 (84.86%), Postives = 507/555 (91.35%), Query Frame = 0


Query: 1   MVSSNPISLLTFLTLLFLLSPSSSA-APTADICQSTTSPPFCNSFLPKSPDSIHSHCRFT 60
           MVSSN ISLLTFLT LFLL  S SA  PT +IC+STT P FCN+FLP SP S+HSHCRFT
Sbjct: 1   MVSSNSISLLTFLTFLFLLPSSFSAVVPTTEICRSTTFPSFCNAFLPNSPASVHSHCRFT 60

Query: 61  LHQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYD 120
           LH AL HA+TFL+LV+A L+L P  SALHDCR LAEANLD++ QTFSIVNST+  LPYY 
Sbjct: 61  LHHALTHAQTFLALVDAHLSLFPFPSALHDCRSLAEANLDYITQTFSIVNSTSVALPYYQ 120

Query: 121 AHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK 180
           AHEM+SL+SA+ITN +TCYEGLA L SA+GLVDKVLEAISFD+KLYSLYLSLFKMGWVSK
Sbjct: 121 AHEMMSLMSAMITNENTCYEGLAGLGSAIGLVDKVLEAISFDRKLYSLYLSLFKMGWVSK 180

Query: 181 DLKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVN 240
           ++KAP  PKMNHFG GKGQLKLKMSPKDRAYYERLVH  KP  ARRLLQTNY++DGI+VN
Sbjct: 181 NMKAPILPKMNHFGVGKGQLKLKMSPKDRAYYERLVHSKKPAAARRLLQTNYENDGILVN 240

Query: 241 GIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIG 300
           G+V VDQNG YDFTNI+AAIAAAP+KTTV KGYFLIFV AGIYNETVLVPK KRYVLLIG
Sbjct: 241 GVVAVDQNGTYDFTNISAAIAAAPDKTTVTKGYFLIFVTAGIYNETVLVPKNKRYVLLIG 300

Query: 301 EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADN 360
           EGNNQTII GNKNVVDGSTTFNSATVAVEGTGF GVNLTI+NTAG AKHQAVALRVSADN
Sbjct: 301 EGNNQTIINGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGPAKHQAVALRVSADN 360

Query: 361 VTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFN 420
            TLY+CIFEGYQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLP+SGQFN
Sbjct: 361 ATLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFN 420

Query: 421 ALTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI 480
           ALTAQGRTDPNQNTGTSIHNCTIKATPELAA+PATKSYLGRPWK+YSRTVYMQSFID+FI
Sbjct: 421 ALTAQGRTDPNQNTGTSIHNCTIKATPELAANPATKSYLGRPWKEYSRTVYMQSFIDAFI 480

Query: 481 DPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDK 540
           DP GWKEWDGTLNLNTSYYAEFNNSGPG DTS+RA W VGVINATVA++FTVSQ LAGD+
Sbjct: 481 DPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRAPWVVGVINATVANSFTVSQFLAGDQ 540

Query: 541 WLPPTEVPYTGGLIS 555
           WLPPT VPYTGGLIS
Sbjct: 541 WLPPTAVPYTGGLIS 555

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BLAST of CsaV3_2G017610 vs. NCBI nr
Match: KAG6571391.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 878.2 bits (2268), Expect = 3.6e-251
Identity = 436/554 (78.70%), Postives = 483/554 (87.18%), Query Frame = 0


Query: 3   SSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQ 62
           SSNPISLL F +LLFL + SSSA PT ++CQS TSP FC SFLP SP S+HSHCR+TLH 
Sbjct: 4   SSNPISLLLFFSLLFLFT-SSSAVPTVEVCQSATSPSFCKSFLPSSPASVHSHCRYTLHH 63

Query: 63  ALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHE 122
           AL  ARTF++LV+ QL L PSLSALHDCR LAEANLDF+  T   VN+TT  LP+Y A E
Sbjct: 64  ALVQARTFVTLVDLQLTLFPSLSALHDCRSLAEANLDFISSTVDTVNTTTNALPFYQADE 123

Query: 123 MLSLISAIITNVDTCYEGLA--SLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKD 182
           M+SL+SA+ITNVDTC++GLA     S +GLVDKV++AISFD++LYSLYLSLFKMGWVSK+
Sbjct: 124 MISLLSAVITNVDTCHDGLAGSGSGSGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVSKN 183

Query: 183 LKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNG 242
           +KAP  PK NHFG G G LKLKMSPKDRAYYERLVH  K P ARRLLQTNY DDGI+VNG
Sbjct: 184 MKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLVH-GKKPAARRLLQTNYADDGILVNG 243

Query: 243 IVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGE 302
           +V VDQNG YDFT I  AIAAAPNK+T A GYFLIFV AGIY E+V+VPK K+YVL+IGE
Sbjct: 244 VVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGE 303

Query: 303 GNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNV 362
           GNN+TIITGNKNVVDGSTTFNSATV VEGTGF G NLTITNTAG+ KHQAVALRVSAD  
Sbjct: 304 GNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMT 363

Query: 363 TLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNA 422
           T Y+CIFEGYQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLP+SGQFNA
Sbjct: 364 TFYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNA 423

Query: 423 LTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFID 482
           LTAQGRTD NQNTG  IH CTIK TP+LAASPATKSYLGRPWK++SRTVYMQSFID+FID
Sbjct: 424 LTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPATKSYLGRPWKEFSRTVYMQSFIDAFID 483

Query: 483 PVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKW 542
           P GWKEWDGTLNLNTSYYAEF+NSGPG DTS+RA WAVGVINATVA+ FTVS++LAGD+W
Sbjct: 484 PAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATVANIFTVSEILAGDQW 543

Query: 543 LPPTEVPYTGGLIS 555
           LPPT VPY+GGLIS
Sbjct: 544 LPPTAVPYSGGLIS 555

[Close]

BLAST of CsaV3_2G017610 vs. NCBI nr
Match: XP_022928019.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 876.3 bits (2263), Expect = 1.4e-250
Identity = 432/552 (78.26%), Postives = 479/552 (86.78%), Query Frame = 0


Query: 3   SSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQ 62
           SSNPISLL F +LLFL + SSSA PT ++CQS TSP FC SFLP SP S+HSHCR+TLH 
Sbjct: 4   SSNPISLLLFFSLLFLFT-SSSAVPTVEVCQSATSPSFCKSFLPSSPASVHSHCRYTLHH 63

Query: 63  ALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHE 122
           AL  ARTF++LV+ QL L PSLSALHDCR LA ANLDF+  T   VN+TT  LP+Y A E
Sbjct: 64  ALVQARTFVTLVDLQLTLFPSLSALHDCRSLAVANLDFISSTVDTVNTTTNALPFYQADE 123

Query: 123 MLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDLK 182
           M+SL+SA+ITN+DTC++GLA     +G VDKV++AISFD++LYSLYLSLFKMGWVSK++K
Sbjct: 124 MISLLSAVITNIDTCHDGLAGSGLGLGFVDKVVKAISFDRQLYSLYLSLFKMGWVSKNMK 183

Query: 183 APTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNGIV 242
           AP  PK NHFG GKG LKLKMSPKDRAYYERLVH  K P ARRLLQTNY DDGI+VNG+V
Sbjct: 184 APKLPKTNHFGFGKGPLKLKMSPKDRAYYERLVH-GKKPAARRLLQTNYADDGILVNGVV 243

Query: 243 GVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGEGN 302
            VDQNG YDFT I  AIAAAPNK+T A GYFLIFV AGIY E+V+VPK K+YVL+IGEGN
Sbjct: 244 AVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGEGN 303

Query: 303 NQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVTL 362
           N+TIITGNKNVVDGSTTFNSATV VEGTGF G NLTITNTAG+ KHQAVALRVSAD  T 
Sbjct: 304 NRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMTTF 363

Query: 363 YNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALT 422
           Y+CIFEGYQDTLY HSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLP+SGQFNALT
Sbjct: 364 YSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNALT 423

Query: 423 AQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFIDPV 482
           AQGRTD NQNTG  IH CTIK TP+LAASP TKSYLGRPWK++SRTVYMQSFID+FIDP 
Sbjct: 424 AQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFIDAFIDPA 483

Query: 483 GWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKWLP 542
           GWKEWDGTLNLNTSYYAEF+NSGPG DTSQRA WAVGVINATVA+ FTVS++LAGD+WLP
Sbjct: 484 GWKEWDGTLNLNTSYYAEFDNSGPGSDTSQRAQWAVGVINATVANIFTVSEILAGDQWLP 543

Query: 543 PTEVPYTGGLIS 555
           PT VPY+GGLIS
Sbjct: 544 PTAVPYSGGLIS 553

[Close]

BLAST of CsaV3_2G017610 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 518.1 bits (1333), Expect = 1.2e-145
Identity = 282/544 (51.84%), Postives = 359/544 (65.99%), Query Frame = 0


Query: 24  SAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQALAHARTFLSLVNAQLN---- 83
           +A   ++IC+    P +C S LP  P  I+S+ R +L ++L+ AR F+S+++A+L+    
Sbjct: 31  NATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGK 90

Query: 84  --LLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHEMLSLISAIITNVDTC 143
                ++ AL DC+ LA   +D+L  +    +S T TL    A ++ + +SA ITN  TC
Sbjct: 91  VAAKSTVGALEDCKFLASLTMDYLLSSSQTADS-TKTLSLSRAEDVHTFLSAAITNEQTC 150

Query: 144 YEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWV-----SKDLKAPTFPKMNHF 203
            EGL S  S  GL   +      D KLY + L+LF  GWV     S+ +  P       F
Sbjct: 151 LEGLKSTASENGLSGDLFN----DTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFF 210

Query: 204 GAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNGIVGVDQNGMYDF 263
           G   G+L LKM+ + RA Y  +         R+LLQ++   D + V+ IV V QNG  +F
Sbjct: 211 GFRNGKLPLKMTERARAVYNTVTR-------RKLLQSD--ADAVQVSDIVTVIQNGTGNF 270

Query: 264 TNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGEGNNQTIITGNKN 323
           T I AAIAAAPNKT  + GYFLI+V AG+Y E V VPK KRYV++IG+G NQT+ITGN++
Sbjct: 271 TTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRS 330

Query: 324 VVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVTLYNCIFEGYQD 383
           VVDG TTFNSAT  + G  F+GVN+TI NTAG  K QAVALR   D    Y+C FE YQD
Sbjct: 331 VVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQD 390

Query: 384 TLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQN 443
           TLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCN+Y R P  GQ N +TAQGRTDPNQN
Sbjct: 391 TLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQN 450

Query: 444 TGTSIHNCTIKATPELAASPAT-KSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTL 503
           TGT+IH CTI+   +LA S  T K+YLGRPWK+YSRTV MQ++ID F++P GW  W G  
Sbjct: 451 TGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDF 510

Query: 504 NLNTSYYAEFNNSGPGCDTSQRASW-AVGVINATVASNFTVSQLLAGDKWLPPTEVPYTG 555
            L+T YYAE+NN+GPG DT+ R +W    VINAT ASNFTV+  L G+ W+  T VP+ G
Sbjct: 511 ALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFVG 560

[Close]

BLAST of CsaV3_2G017610 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 1.6e-140
Identity = 285/581 (49.05%), Postives = 373/581 (64.20%), Query Frame = 0


Query: 5   NPISLLTFLT--LLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQ 64
           +PI +L  L+  L  +L+ S + + ++ IC++T  P +C S  P S  ++  +  F++ +
Sbjct: 3   SPIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRK 62

Query: 65  ALAHARTFLSLV------NAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTT--- 124
           +L+ +R F+  V      NA L+    + AL DCR LA   +D+L  +F  VN T+    
Sbjct: 63  SLSQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTS 122

Query: 125 --TLPYYDAHEMLSLISAIITNVDTCYEGL---ASLNSAVGLVDKVLEAISFDKKLYSLY 184
              L +  A ++ +L+SA +TN  TC EGL   AS ++   +   V   +  D KL  + 
Sbjct: 123 FKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVS 182

Query: 185 LSLFKMGWVSKDLKAPTF----PKMNHFGAGK-------GQLKLKMSPKDRAYYERLVHR 244
           L+LF  GWV K  K   F    P+       K       G L LKM+ K +A YE L  R
Sbjct: 183 LALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRR 242

Query: 245 NKPPGARRLLQTNYQDDG--IVVNGIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLI 304
               G          DDG  ++++ IV V Q+G  +FTNITAA+AAAPN T  + G+FLI
Sbjct: 243 KLADGD----SNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLI 302

Query: 305 FVAAGIYNETVLVPKEKRYVLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGV 364
           +V AGIY E + + K KRY+++IG+G NQT++TGN++VVDG TTFNSAT AV    F+ V
Sbjct: 303 YVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAV 362

Query: 365 NLTITNTAGSAKHQAVALRVSADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFI 424
           N+T  NTAG  KHQAVALR  AD    Y+C FE YQDTLYTHSLRQFYRECDVYGTVDFI
Sbjct: 363 NITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFI 422

Query: 425 FGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASPAT- 484
           FGNAAVV QNCN+Y R PM  QFNA+TAQGR+DPNQNTGTSI NCTIK   +L +S  T 
Sbjct: 423 FGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTV 482

Query: 485 KSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRA 544
           K+YLGRPWK+YSRTVYMQS+ID F++PVGW+EW+G   L+T YYAE+NN+GPG +T+ R 
Sbjct: 483 KTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRV 542

Query: 545 SW-AVGVINATVASNFTVSQLLAGDKWLPPTEVPYTGGLIS 555
           +W    VIN+T A+NFTV+ L     W+  T VPYT GLIS
Sbjct: 543 TWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLIS 579

[Close]

BLAST of CsaV3_2G017610 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 497.3 bits (1279), Expect = 2.3e-139
Identity = 284/582 (48.80%), Postives = 371/582 (63.75%), Query Frame = 0


Query: 7   ISLLTFLTLLFL-----------LSPSSSAAPTADICQSTTSPPFCNSFLPK-SPDSIHS 66
           +SL  FL  LFL           L PS+S   ++ IC++T  P FC S  P+ S   +  
Sbjct: 2   LSLKLFLVTLFLSLQTLFIASQTLLPSNS---SSTICKTTPDPKFCKSVFPQTSQGDVRE 61

Query: 67  HCRFTLHQALAHARTFLSLVNAQL---NLLPSLS---ALHDCRCLAEANLDFLFQTFSIV 126
           + RF+L ++L  +R F   ++  L   N L S S   AL DCR LA    D+L  +F  V
Sbjct: 62  YGRFSLRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETV 121

Query: 127 NSTTT--TLPYYDAHEMLSLISAIITNVDTCYEGL-ASLNSAVGLVDKVLEAISFDKKLY 186
           N TT+  TL +  A E+ +L+SA +TN  TC +G+  + +S+  + + V   +  D KL+
Sbjct: 122 NITTSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLF 181

Query: 187 SLYLSLFKMGWVSKDLK-----APTFPKMNHFGA------GKGQLKLKMSPKDRAYYERL 246
           S+ L+LF  GWV K  K     +   PK  H           G L LKM+   RA YE L
Sbjct: 182 SVSLALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESL 241

Query: 247 VHRNKPPGARRLLQTNYQDDGIVVNGIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFL 306
                    R+L   +   + ++V+ IV V+QNG  +FT IT A+ +APNKT    GYF+
Sbjct: 242 -------SRRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFV 301

Query: 307 IFVAAGIYNETVLVPKEKRYVLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLG 366
           I+V +G+Y E V++ K KRY+++IG+G N+T++TGN+NVVDG TTFNSAT AV    F+ 
Sbjct: 302 IYVTSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVA 361

Query: 367 VNLTITNTAGSAKHQAVALRVSADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDF 426
           VN+T  NTAG  KHQAVA+R SAD    Y+C FE YQDTLYTHSLRQFYRECD+YGTVDF
Sbjct: 362 VNMTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDF 421

Query: 427 IFGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASPAT 486
           IFGNAAVV Q+CN+Y R PM  QFNA+TAQGRTDPNQNTG SIHNCTIK   +L +S  T
Sbjct: 422 IFGNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYT 481

Query: 487 -KSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQR 546
            K+YLGRPWK+YSRTV+MQS+ID  ++PVGW+EW+G   L+T YYAE+NN+G G  T+ R
Sbjct: 482 VKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDR 541

Query: 547 ASW-AVGVINATVASNFTVSQLLAGDKWLPPTEVPYTGGLIS 555
             W    VIN+T A+NFTV   L GD W+  + VPY  GL+S
Sbjct: 542 VVWPGYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLLS 573

[Close]

BLAST of CsaV3_2G017610 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 2.5e-114
Identity = 242/560 (43.21%), Postives = 332/560 (59.29%), Query Frame = 0


Query: 19  LSPSSSAAPTADICQSTTSPPFCNSFL---PKSPDSIHSHCRFTLHQALAHARTFLSLVN 78
           L PS S +P+   C+ST  P  C + L     SP   + + +FT+ Q L  A     ++ 
Sbjct: 71  LPPSQSQSPSL-ACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVIT 130

Query: 79  AQLNLLPS---------LSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHEMLSL 138
           +    + S         + A+ DC  L+E ++++L +T +    T   +       + SL
Sbjct: 131 SYARRVESKPGSATAEEIGAVADCGELSELSVNYL-ETVTTELKTAQVMTAALVEHVNSL 190

Query: 139 ISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDLKAPTF 198
           +S ++TN  TC +GL    S  G    +   +    +LYS+ L L     ++++LK    
Sbjct: 191 LSGVVTNQQTCLDGLVEAKS--GFAAAIGSPMGNLTRLYSISLGLVSHA-LNRNLK---- 250

Query: 199 PKMNHFGAGKGQLKLKMSPKDRAYYERLV---------HRNKPPGARRLLQTNYQDDG-I 258
                F A KG++    +   R   E L+          ++    +R L +      G I
Sbjct: 251 ----RFKASKGKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSI 310

Query: 259 VVNGIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVL 318
           +V+  V V      +FT IT AIAAAPN T    GYF+I+   G+Y E ++VP  K+ ++
Sbjct: 311 LVSKAVIVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLM 370

Query: 319 LIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVS 378
           L+G+G N+TIITGN NVVDG TT+N ++ AV G  F+ V++T  NTAG  KHQAVALR +
Sbjct: 371 LMGDGINKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNN 430

Query: 379 ADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSG 438
           A+  + Y C FEGYQDTLY HSLRQFYRECD+YGTVDFIFGNAA + QNCNIYAR PM+ 
Sbjct: 431 AEGSSFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAK 490

Query: 439 QFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASP-ATKSYLGRPWKQYSRTVYMQSFI 498
           Q NA+TA GR DPNQNTG SI NCTIKA P+LAA P +  ++LGRPWK YSRTV+MQS+I
Sbjct: 491 QKNAITAHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYI 550

Query: 499 DSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASW-AVGVINATVASNFTVSQL 555
              + PVGW EW+GT+ L+T YY E++N GPG +T+QR  W    ++N   A NFTV   
Sbjct: 551 SDIVQPVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNF 610

[Close]

BLAST of CsaV3_2G017610 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 9.6e-114
Identity = 240/551 (43.56%), Postives = 326/551 (59.17%), Query Frame = 0


Query: 20  SPSS-SAAPTADICQSTTSPPFCNSFL---PKSPDSIHSHCRFTLHQALAHARTFLSLVN 79
           SPS  S  P    C+ST  P  C + L     SP   + + +FT+ Q L  AR    ++N
Sbjct: 75  SPSQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVIN 134

Query: 80  --AQ-------LNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHEMLSL 139
             AQ        + +  +SA+ DC  LAE ++++L +T +        +       + SL
Sbjct: 135 RFAQRVEADPGTSTVEEVSAVADCGELAELSVEYL-ETVTEELKAAELMTAALVDRVTSL 194

Query: 140 ISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDLKAPTF 199
           +  ++TN  TC +GL    S  G    +   +    +LYS+ L L     ++++LK    
Sbjct: 195 LGGVVTNQQTCLDGLVDAKS--GFATAIGTPLGNLTRLYSVSLGLVSHA-LNRNLKRYKG 254

Query: 200 PKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDG--IVVNGIVGV 259
            K   FG G   ++  +    +   +          A R L    +  G  I+V   V V
Sbjct: 255 SKGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTV 314

Query: 260 DQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGEGNNQ 319
                 +F  IT A+AAAPN T   +GYF+I+  AG+Y E V++  +KR ++LIG+G N+
Sbjct: 315 GPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINK 374

Query: 320 TIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVTLYN 379
           TII+GN + +DG TT+NS+T AV G  F+ V++T  NTAG  KHQAVA+R +AD  T Y 
Sbjct: 375 TIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYR 434

Query: 380 CIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALTAQ 439
           C FEGYQDTLY HSLRQFYRECD+YGT+DFIFGNAA + QNCNIYAR PM+ Q NA+TA 
Sbjct: 435 CSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAH 494

Query: 440 GRTDPNQNTGTSIHNCTIKATPELAASP-ATKSYLGRPWKQYSRTVYMQSFIDSFIDPVG 499
           GRTDPNQ TG SI NCTI A P+LAA P +T ++LGRPWK YSRTVY+QS+I   + PVG
Sbjct: 495 GRTDPNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVG 554

Query: 500 WKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWA-VGVINATVASNFTVSQLLAGDKWLP 554
           W EW+GT  L+T  Y E++N GPG DTS+R  W+   ++N   A NFTV     GD WLP
Sbjct: 555 WLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLP 614

[Close]

BLAST of CsaV3_2G017610 vs. ExPASy TrEMBL
Match: A0A5A7TRW1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002210 PE=3 SV=1)

HSP 1 Score: 1028.9 bits (2659), Expect = 8.0e-297
Identity = 511/555 (92.07%), Postives = 530/555 (95.50%), Query Frame = 0


Query: 1   MVSSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL 60
           MVSSNPIS+LTFLTLLF LSPSSSA PTA+ICQSTTSPPFCN FLP SP S+HSHCRFTL
Sbjct: 1   MVSSNPISVLTFLTLLFFLSPSSSAVPTAEICQSTTSPPFCNFFLPNSPASVHSHCRFTL 60

Query: 61  HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTT-LPYYD 120
           H AL HARTFLSLVNA LNL PSLS LHDCR LAEANLDFL QTFSIVNSTTTT LPYYD
Sbjct: 61  HHALVHARTFLSLVNAHLNLFPSLSPLHDCRSLAEANLDFLSQTFSIVNSTTTTLLPYYD 120

Query: 121 AHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK 180
           AHE L+L+SA+ITN DTCYEGLA L+SAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK
Sbjct: 121 AHEKLTLMSAVITNEDTCYEGLAGLDSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK 180

Query: 181 DLKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVN 240
           DLKAPT PKM HFG GKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIV+N
Sbjct: 181 DLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVIN 240

Query: 241 GIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIG 300
           GIVGVDQNG YDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVL+PKEKRYVLLIG
Sbjct: 241 GIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLIPKEKRYVLLIG 300

Query: 301 EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADN 360
           EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTI+NTAG+AKHQAVALRVSADN
Sbjct: 301 EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTAGAAKHQAVALRVSADN 360

Query: 361 VTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFN 420
            TLYNCIFEGYQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLP+SGQFN
Sbjct: 361 ATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFN 420

Query: 421 ALTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI 480
           A+TAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI
Sbjct: 421 AVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI 480

Query: 481 DPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDK 540
           DP GWKEWDGT+NLNTSYYAEFNNSGPG DTSQR SWAVGVINATVA++FTVSQ LAGD+
Sbjct: 481 DPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINATVANSFTVSQFLAGDR 540

Query: 541 WLPPTEVPYTGGLIS 555
           WLPPT+VPYTGGLIS
Sbjct: 541 WLPPTDVPYTGGLIS 555

[Close]

BLAST of CsaV3_2G017610 vs. ExPASy TrEMBL
Match: A0A1S3C644 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497470 PE=3 SV=1)

HSP 1 Score: 1028.9 bits (2659), Expect = 8.0e-297
Identity = 511/555 (92.07%), Postives = 530/555 (95.50%), Query Frame = 0


Query: 1   MVSSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL 60
           MVSSNPIS+LTFLTLLF LSPSSSA PTA+ICQSTTSPPFCN FLP SP S+HSHCRFTL
Sbjct: 1   MVSSNPISVLTFLTLLFFLSPSSSAVPTAEICQSTTSPPFCNFFLPNSPASVHSHCRFTL 60

Query: 61  HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTT-LPYYD 120
           H AL HARTFLSLVNA LNL PSLS LHDCR LAEANLDFL QTFSIVNSTTTT LPYYD
Sbjct: 61  HHALVHARTFLSLVNAHLNLFPSLSPLHDCRSLAEANLDFLSQTFSIVNSTTTTLLPYYD 120

Query: 121 AHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK 180
           AHE L+L+SA+ITN DTCYEGLA L+SAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK
Sbjct: 121 AHEKLTLMSAVITNEDTCYEGLAGLDSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK 180

Query: 181 DLKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVN 240
           DLKAPT PKM HFG GKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIV+N
Sbjct: 181 DLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVIN 240

Query: 241 GIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIG 300
           GIVGVDQNG YDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVL+PKEKRYVLLIG
Sbjct: 241 GIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLIPKEKRYVLLIG 300

Query: 301 EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADN 360
           EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTI+NTAG+AKHQAVALRVSADN
Sbjct: 301 EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTAGAAKHQAVALRVSADN 360

Query: 361 VTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFN 420
            TLYNCIFEGYQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLP+SGQFN
Sbjct: 361 ATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFN 420

Query: 421 ALTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI 480
           A+TAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI
Sbjct: 421 AVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI 480

Query: 481 DPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDK 540
           DP GWKEWDGT+NLNTSYYAEFNNSGPG DTSQR SWAVGVINATVA++FTVSQ LAGD+
Sbjct: 481 DPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINATVANSFTVSQFLAGDR 540

Query: 541 WLPPTEVPYTGGLIS 555
           WLPPT+VPYTGGLIS
Sbjct: 541 WLPPTDVPYTGGLIS 555

[Close]

BLAST of CsaV3_2G017610 vs. ExPASy TrEMBL
Match: A0A6J1EJM8 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434929 PE=3 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 6.7e-251
Identity = 432/552 (78.26%), Postives = 479/552 (86.78%), Query Frame = 0


Query: 3   SSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQ 62
           SSNPISLL F +LLFL + SSSA PT ++CQS TSP FC SFLP SP S+HSHCR+TLH 
Sbjct: 4   SSNPISLLLFFSLLFLFT-SSSAVPTVEVCQSATSPSFCKSFLPSSPASVHSHCRYTLHH 63

Query: 63  ALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHE 122
           AL  ARTF++LV+ QL L PSLSALHDCR LA ANLDF+  T   VN+TT  LP+Y A E
Sbjct: 64  ALVQARTFVTLVDLQLTLFPSLSALHDCRSLAVANLDFISSTVDTVNTTTNALPFYQADE 123

Query: 123 MLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDLK 182
           M+SL+SA+ITN+DTC++GLA     +G VDKV++AISFD++LYSLYLSLFKMGWVSK++K
Sbjct: 124 MISLLSAVITNIDTCHDGLAGSGLGLGFVDKVVKAISFDRQLYSLYLSLFKMGWVSKNMK 183

Query: 183 APTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNGIV 242
           AP  PK NHFG GKG LKLKMSPKDRAYYERLVH  K P ARRLLQTNY DDGI+VNG+V
Sbjct: 184 APKLPKTNHFGFGKGPLKLKMSPKDRAYYERLVH-GKKPAARRLLQTNYADDGILVNGVV 243

Query: 243 GVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGEGN 302
            VDQNG YDFT I  AIAAAPNK+T A GYFLIFV AGIY E+V+VPK K+YVL+IGEGN
Sbjct: 244 AVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGEGN 303

Query: 303 NQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVTL 362
           N+TIITGNKNVVDGSTTFNSATV VEGTGF G NLTITNTAG+ KHQAVALRVSAD  T 
Sbjct: 304 NRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMTTF 363

Query: 363 YNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALT 422
           Y+CIFEGYQDTLY HSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLP+SGQFNALT
Sbjct: 364 YSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNALT 423

Query: 423 AQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFIDPV 482
           AQGRTD NQNTG  IH CTIK TP+LAASP TKSYLGRPWK++SRTVYMQSFID+FIDP 
Sbjct: 424 AQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFIDAFIDPA 483

Query: 483 GWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKWLP 542
           GWKEWDGTLNLNTSYYAEF+NSGPG DTSQRA WAVGVINATVA+ FTVS++LAGD+WLP
Sbjct: 484 GWKEWDGTLNLNTSYYAEFDNSGPGSDTSQRAQWAVGVINATVANIFTVSEILAGDQWLP 543

Query: 543 PTEVPYTGGLIS 555
           PT VPY+GGLIS
Sbjct: 544 PTAVPYSGGLIS 553

[Close]

BLAST of CsaV3_2G017610 vs. ExPASy TrEMBL
Match: A0A6J1I6B8 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470332 PE=3 SV=1)

HSP 1 Score: 870.5 bits (2248), Expect = 3.7e-249
Identity = 433/553 (78.30%), Postives = 480/553 (86.80%), Query Frame = 0


Query: 3   SSNPISLLTFLTLLFL-LSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLH 62
           SSNPISLLTF +LLFL L  SSSA PT ++CQS TSP FC SFLP SP S+HSHCR+TLH
Sbjct: 4   SSNPISLLTFFSLLFLFLFTSSSAVPTVEVCQSATSPSFCKSFLPSSPASVHSHCRYTLH 63

Query: 63  QALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAH 122
            AL  ARTF++LV+ QL LLPS SALHDCR LA ANLDF+  T + VN+TT  LP+Y A 
Sbjct: 64  HALVQARTFITLVDLQLTLLPSFSALHDCRSLAMANLDFISSTVNTVNTTTNALPFYQAD 123

Query: 123 EMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDL 182
           EM+SL+SA+ITNVDTC++GLA   S +GLVDKV++AISFD++LYSLYLSLFKMGWVSK +
Sbjct: 124 EMISLLSAVITNVDTCHDGLA--GSGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVSKTM 183

Query: 183 KAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNGI 242
           KAP  PK NHFG G G LKLKMSPKDRAYYERL+H  K P ARRLLQTN  DDGI+VNG+
Sbjct: 184 KAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMH-GKKPAARRLLQTNNADDGILVNGV 243

Query: 243 VGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGEG 302
           V VDQNG YDFT I  AIAAAPNK+T A GYFLIFV AGIY E+V+VPK K+YVL+IGEG
Sbjct: 244 VAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGEG 303

Query: 303 NNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVT 362
           NN+TIITGNKNVVDGSTTFNSATV VEGTGF G NLTITNTAG+ KHQAVALRVSAD  T
Sbjct: 304 NNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMTT 363

Query: 363 LYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNAL 422
            Y+CIFEGYQDTLY HSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLP+SGQFNAL
Sbjct: 364 FYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNAL 423

Query: 423 TAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFIDP 482
           TAQGRTD NQNTG  IH CTIK TP+LAASP TKSYLGRPWK++SRTVYMQSFID+FIDP
Sbjct: 424 TAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFIDAFIDP 483

Query: 483 VGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKWL 542
            GWKEWDGTLNLNTSYYAEF+NSGPG DTS+RA WAVGVINATVA+ FTVS++LAGD+WL
Sbjct: 484 AGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATVANIFTVSEILAGDQWL 543

Query: 543 PPTEVPYTGGLIS 555
           PPT VPY+GGLIS
Sbjct: 544 PPTAVPYSGGLIS 553

[Close]

BLAST of CsaV3_2G017610 vs. ExPASy TrEMBL
Match: A0A6J1L6X0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499728 PE=3 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 3.1e-240
Identity = 428/563 (76.02%), Postives = 469/563 (83.30%), Query Frame = 0


Query: 1   MVSSNPISLLTFLTLLFLLSPSSSA----APTADICQSTTSPPFCNSFLPKSPDSIHSHC 60
           M SS+ I LLTFLTLL+L S SSSA     P ADIC STTSP FC +FLP S  ++HSHC
Sbjct: 1   MGSSSAIPLLTFLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60

Query: 61  RFTLHQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTT-- 120
           RFTL  ALAHARTFL+LV+  L L PSL+ALHDCR LA ANLDFL  TF  VN+TTTT  
Sbjct: 61  RFTLRHALAHARTFLALVDTHLTLFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTTT 120

Query: 121 --LPYYDAHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLF 180
             LP+Y A EM+SL+SA+ITN +TCYEGL S  SA GLVDKVL+AISFD KLYSLYLSLF
Sbjct: 121 NALPFYKAEEMVSLLSAVITNENTCYEGLMSSGSASGLVDKVLKAISFDTKLYSLYLSLF 180

Query: 181 KMGWVSKDLKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTN-Y 240
           KMGWVS D+KAP  PKM HFG GKGQLKL MSPK+RAYYERL+HR+KPP ARRLLQTN  
Sbjct: 181 KMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRSKPP-ARRLLQTNSN 240

Query: 241 QDDGIVVNGIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKE 300
           +DDGI+VNGIV VDQNG YDFT I AAI AAPN +  A GYFLI V AG+YNE+V+VPK 
Sbjct: 241 EDDGILVNGIVAVDQNGTYDFTTINAAIQAAPNNSVAADGYFLIIVTAGVYNESVMVPKH 300

Query: 301 KRYVLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAV 360
           K+YVL+IGEGNNQTIITGNKNVVDGSTTFNS T  VEGTGF GVNLTITNTAG  K QAV
Sbjct: 301 KKYVLMIGEGNNQTIITGNKNVVDGSTTFNSGTFIVEGTGFFGVNLTITNTAGPNKFQAV 360

Query: 361 ALRVSADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYAR 420
           ALRVSAD  T Y+CIFEGYQDTLY HSLRQFYRECD+YGT+DFI G+AAVV QNCNIYAR
Sbjct: 361 ALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYAR 420

Query: 421 LPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYM 480
           LPM GQ+  +TAQGRTD NQNTG SIHNCTIK T +LAASP  KSY GRPWK+Y+RTVYM
Sbjct: 421 LPMKGQYIVITAQGRTDRNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTVYM 480

Query: 481 QSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTV 540
           QSFID+FIDP GW  WDGTLNLNTSYYAEFNNSGPG DTS+RA WAVG+INATVA+NFTV
Sbjct: 481 QSFIDAFIDPEGWNVWDGTLNLNTSYYAEFNNSGPGSDTSRRAKWAVGLINATVANNFTV 540

Query: 541 SQLLAGDKWLPPTEVPYTGGLIS 555
           S  L GD+WLPPT VPYTGGLIS
Sbjct: 541 SPFLGGDQWLPPTTVPYTGGLIS 562

[Close]

BLAST of CsaV3_2G017610 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 518.1 bits (1333), Expect = 8.9e-147
Identity = 282/544 (51.84%), Postives = 359/544 (65.99%), Query Frame = 0


Query: 24  SAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQALAHARTFLSLVNAQLN---- 83
           +A   ++IC+    P +C S LP  P  I+S+ R +L ++L+ AR F+S+++A+L+    
Sbjct: 31  NATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGK 90

Query: 84  --LLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHEMLSLISAIITNVDTC 143
                ++ AL DC+ LA   +D+L  +    +S T TL    A ++ + +SA ITN  TC
Sbjct: 91  VAAKSTVGALEDCKFLASLTMDYLLSSSQTADS-TKTLSLSRAEDVHTFLSAAITNEQTC 150

Query: 144 YEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWV-----SKDLKAPTFPKMNHF 203
            EGL S  S  GL   +      D KLY + L+LF  GWV     S+ +  P       F
Sbjct: 151 LEGLKSTASENGLSGDLFN----DTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFF 210

Query: 204 GAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNGIVGVDQNGMYDF 263
           G   G+L LKM+ + RA Y  +         R+LLQ++   D + V+ IV V QNG  +F
Sbjct: 211 GFRNGKLPLKMTERARAVYNTVTR-------RKLLQSD--ADAVQVSDIVTVIQNGTGNF 270

Query: 264 TNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGEGNNQTIITGNKN 323
           T I AAIAAAPNKT  + GYFLI+V AG+Y E V VPK KRYV++IG+G NQT+ITGN++
Sbjct: 271 TTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRS 330

Query: 324 VVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVTLYNCIFEGYQD 383
           VVDG TTFNSAT  + G  F+GVN+TI NTAG  K QAVALR   D    Y+C FE YQD
Sbjct: 331 VVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQD 390

Query: 384 TLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQN 443
           TLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCN+Y R P  GQ N +TAQGRTDPNQN
Sbjct: 391 TLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQN 450

Query: 444 TGTSIHNCTIKATPELAASPAT-KSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTL 503
           TGT+IH CTI+   +LA S  T K+YLGRPWK+YSRTV MQ++ID F++P GW  W G  
Sbjct: 451 TGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDF 510

Query: 504 NLNTSYYAEFNNSGPGCDTSQRASW-AVGVINATVASNFTVSQLLAGDKWLPPTEVPYTG 555
            L+T YYAE+NN+GPG DT+ R +W    VINAT ASNFTV+  L G+ W+  T VP+ G
Sbjct: 511 ALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFVG 560

[Close]

BLAST of CsaV3_2G017610 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 501.1 bits (1289), Expect = 1.1e-141
Identity = 285/581 (49.05%), Postives = 373/581 (64.20%), Query Frame = 0


Query: 5   NPISLLTFLT--LLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQ 64
           +PI +L  L+  L  +L+ S + + ++ IC++T  P +C S  P S  ++  +  F++ +
Sbjct: 3   SPIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRK 62

Query: 65  ALAHARTFLSLV------NAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTT--- 124
           +L+ +R F+  V      NA L+    + AL DCR LA   +D+L  +F  VN T+    
Sbjct: 63  SLSQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTS 122

Query: 125 --TLPYYDAHEMLSLISAIITNVDTCYEGL---ASLNSAVGLVDKVLEAISFDKKLYSLY 184
              L +  A ++ +L+SA +TN  TC EGL   AS ++   +   V   +  D KL  + 
Sbjct: 123 FKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVS 182

Query: 185 LSLFKMGWVSKDLKAPTF----PKMNHFGAGK-------GQLKLKMSPKDRAYYERLVHR 244
           L+LF  GWV K  K   F    P+       K       G L LKM+ K +A YE L  R
Sbjct: 183 LALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRR 242

Query: 245 NKPPGARRLLQTNYQDDG--IVVNGIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLI 304
               G          DDG  ++++ IV V Q+G  +FTNITAA+AAAPN T  + G+FLI
Sbjct: 243 KLADGD----SNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLI 302

Query: 305 FVAAGIYNETVLVPKEKRYVLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGV 364
           +V AGIY E + + K KRY+++IG+G NQT++TGN++VVDG TTFNSAT AV    F+ V
Sbjct: 303 YVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAV 362

Query: 365 NLTITNTAGSAKHQAVALRVSADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFI 424
           N+T  NTAG  KHQAVALR  AD    Y+C FE YQDTLYTHSLRQFYRECDVYGTVDFI
Sbjct: 363 NITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFI 422

Query: 425 FGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASPAT- 484
           FGNAAVV QNCN+Y R PM  QFNA+TAQGR+DPNQNTGTSI NCTIK   +L +S  T 
Sbjct: 423 FGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTV 482

Query: 485 KSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRA 544
           K+YLGRPWK+YSRTVYMQS+ID F++PVGW+EW+G   L+T YYAE+NN+GPG +T+ R 
Sbjct: 483 KTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRV 542

Query: 545 SW-AVGVINATVASNFTVSQLLAGDKWLPPTEVPYTGGLIS 555
           +W    VIN+T A+NFTV+ L     W+  T VPYT GLIS
Sbjct: 543 TWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLIS 579

[Close]

BLAST of CsaV3_2G017610 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 497.3 bits (1279), Expect = 1.6e-140
Identity = 284/582 (48.80%), Postives = 371/582 (63.75%), Query Frame = 0


Query: 7   ISLLTFLTLLFL-----------LSPSSSAAPTADICQSTTSPPFCNSFLPK-SPDSIHS 66
           +SL  FL  LFL           L PS+S   ++ IC++T  P FC S  P+ S   +  
Sbjct: 2   LSLKLFLVTLFLSLQTLFIASQTLLPSNS---SSTICKTTPDPKFCKSVFPQTSQGDVRE 61

Query: 67  HCRFTLHQALAHARTFLSLVNAQL---NLLPSLS---ALHDCRCLAEANLDFLFQTFSIV 126
           + RF+L ++L  +R F   ++  L   N L S S   AL DCR LA    D+L  +F  V
Sbjct: 62  YGRFSLRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETV 121

Query: 127 NSTTT--TLPYYDAHEMLSLISAIITNVDTCYEGL-ASLNSAVGLVDKVLEAISFDKKLY 186
           N TT+  TL +  A E+ +L+SA +TN  TC +G+  + +S+  + + V   +  D KL+
Sbjct: 122 NITTSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLF 181

Query: 187 SLYLSLFKMGWVSKDLK-----APTFPKMNHFGA------GKGQLKLKMSPKDRAYYERL 246
           S+ L+LF  GWV K  K     +   PK  H           G L LKM+   RA YE L
Sbjct: 182 SVSLALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESL 241

Query: 247 VHRNKPPGARRLLQTNYQDDGIVVNGIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFL 306
                    R+L   +   + ++V+ IV V+QNG  +FT IT A+ +APNKT    GYF+
Sbjct: 242 -------SRRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFV 301

Query: 307 IFVAAGIYNETVLVPKEKRYVLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLG 366
           I+V +G+Y E V++ K KRY+++IG+G N+T++TGN+NVVDG TTFNSAT AV    F+ 
Sbjct: 302 IYVTSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVA 361

Query: 367 VNLTITNTAGSAKHQAVALRVSADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDF 426
           VN+T  NTAG  KHQAVA+R SAD    Y+C FE YQDTLYTHSLRQFYRECD+YGTVDF
Sbjct: 362 VNMTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDF 421

Query: 427 IFGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASPAT 486
           IFGNAAVV Q+CN+Y R PM  QFNA+TAQGRTDPNQNTG SIHNCTIK   +L +S  T
Sbjct: 422 IFGNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYT 481

Query: 487 -KSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQR 546
            K+YLGRPWK+YSRTV+MQS+ID  ++PVGW+EW+G   L+T YYAE+NN+G G  T+ R
Sbjct: 482 VKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDR 541

Query: 547 ASW-AVGVINATVASNFTVSQLLAGDKWLPPTEVPYTGGLIS 555
             W    VIN+T A+NFTV   L GD W+  + VPY  GL+S
Sbjct: 542 VVWPGYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLLS 573

[Close]

BLAST of CsaV3_2G017610 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 414.1 bits (1063), Expect = 1.8e-115
Identity = 242/560 (43.21%), Postives = 332/560 (59.29%), Query Frame = 0


Query: 19  LSPSSSAAPTADICQSTTSPPFCNSFL---PKSPDSIHSHCRFTLHQALAHARTFLSLVN 78
           L PS S +P+   C+ST  P  C + L     SP   + + +FT+ Q L  A     ++ 
Sbjct: 71  LPPSQSQSPSL-ACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVIT 130

Query: 79  AQLNLLPS---------LSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHEMLSL 138
           +    + S         + A+ DC  L+E ++++L +T +    T   +       + SL
Sbjct: 131 SYARRVESKPGSATAEEIGAVADCGELSELSVNYL-ETVTTELKTAQVMTAALVEHVNSL 190

Query: 139 ISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDLKAPTF 198
           +S ++TN  TC +GL    S  G    +   +    +LYS+ L L     ++++LK    
Sbjct: 191 LSGVVTNQQTCLDGLVEAKS--GFAAAIGSPMGNLTRLYSISLGLVSHA-LNRNLK---- 250

Query: 199 PKMNHFGAGKGQLKLKMSPKDRAYYERLV---------HRNKPPGARRLLQTNYQDDG-I 258
                F A KG++    +   R   E L+          ++    +R L +      G I
Sbjct: 251 ----RFKASKGKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSI 310

Query: 259 VVNGIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVL 318
           +V+  V V      +FT IT AIAAAPN T    GYF+I+   G+Y E ++VP  K+ ++
Sbjct: 311 LVSKAVIVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLM 370

Query: 319 LIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVS 378
           L+G+G N+TIITGN NVVDG TT+N ++ AV G  F+ V++T  NTAG  KHQAVALR +
Sbjct: 371 LMGDGINKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNN 430

Query: 379 ADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSG 438
           A+  + Y C FEGYQDTLY HSLRQFYRECD+YGTVDFIFGNAA + QNCNIYAR PM+ 
Sbjct: 431 AEGSSFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAK 490

Query: 439 QFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASP-ATKSYLGRPWKQYSRTVYMQSFI 498
           Q NA+TA GR DPNQNTG SI NCTIKA P+LAA P +  ++LGRPWK YSRTV+MQS+I
Sbjct: 491 QKNAITAHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYI 550

Query: 499 DSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASW-AVGVINATVASNFTVSQL 555
              + PVGW EW+GT+ L+T YY E++N GPG +T+QR  W    ++N   A NFTV   
Sbjct: 551 SDIVQPVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNF 610

[Close]

BLAST of CsaV3_2G017610 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 412.1 bits (1058), Expect = 6.9e-115
Identity = 240/551 (43.56%), Postives = 326/551 (59.17%), Query Frame = 0


Query: 20  SPSS-SAAPTADICQSTTSPPFCNSFL---PKSPDSIHSHCRFTLHQALAHARTFLSLVN 79
           SPS  S  P    C+ST  P  C + L     SP   + + +FT+ Q L  AR    ++N
Sbjct: 75  SPSQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVIN 134

Query: 80  --AQ-------LNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHEMLSL 139
             AQ        + +  +SA+ DC  LAE ++++L +T +        +       + SL
Sbjct: 135 RFAQRVEADPGTSTVEEVSAVADCGELAELSVEYL-ETVTEELKAAELMTAALVDRVTSL 194

Query: 140 ISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDLKAPTF 199
           +  ++TN  TC +GL    S  G    +   +    +LYS+ L L     ++++LK    
Sbjct: 195 LGGVVTNQQTCLDGLVDAKS--GFATAIGTPLGNLTRLYSVSLGLVSHA-LNRNLKRYKG 254

Query: 200 PKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDG--IVVNGIVGV 259
            K   FG G   ++  +    +   +          A R L    +  G  I+V   V V
Sbjct: 255 SKGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTV 314

Query: 260 DQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGEGNNQ 319
                 +F  IT A+AAAPN T   +GYF+I+  AG+Y E V++  +KR ++LIG+G N+
Sbjct: 315 GPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINK 374

Query: 320 TIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVTLYN 379
           TII+GN + +DG TT+NS+T AV G  F+ V++T  NTAG  KHQAVA+R +AD  T Y 
Sbjct: 375 TIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYR 434

Query: 380 CIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALTAQ 439
           C FEGYQDTLY HSLRQFYRECD+YGT+DFIFGNAA + QNCNIYAR PM+ Q NA+TA 
Sbjct: 435 CSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAH 494

Query: 440 GRTDPNQNTGTSIHNCTIKATPELAASP-ATKSYLGRPWKQYSRTVYMQSFIDSFIDPVG 499
           GRTDPNQ TG SI NCTI A P+LAA P +T ++LGRPWK YSRTVY+QS+I   + PVG
Sbjct: 495 GRTDPNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVG 554

Query: 500 WKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWA-VGVINATVASNFTVSQLLAGDKWLP 554
           W EW+GT  L+T  Y E++N GPG DTS+R  W+   ++N   A NFTV     GD WLP
Sbjct: 555 WLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLP 614

The following BLAST results are available for this feature:

BLAST of CsaV3_2G017610 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148470.1	0.0e+00	100.00	pectinesterase [Cucumis sativus] >KAE8651957.1 hypothetical protein Csa_006047 [...	[more]
XP_008457895.1	1.7e-296	92.07	PREDICTED: pectinesterase-like [Cucumis melo] >KAA0045890.1 pectinesterase-like ...	[more]
XP_038902853.1	4.4e-273	84.86	probable pectinesterase/pectinesterase inhibitor 20 [Benincasa hispida]	[more]
KAG6571391.1	3.6e-251	78.70	putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosp...	[more]
XP_022928019.1	1.4e-250	78.26	pectinesterase-like [Cucurbita moschata]	[more]

BLAST of CsaV3_2G017610 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22256	1.2e-145	51.84	Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3...	[more]
Q9SRX4	1.6e-140	49.05	Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37...	[more]
Q8RXK7	2.3e-139	48.80	Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3...	[more]
Q94CB1	2.5e-114	43.21	Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3...	[more]
Q9FF77	9.6e-114	43.56	Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of CsaV3_2G017610 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TRW1	8.0e-297	92.07	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G00221...	[more]
A0A1S3C644	8.0e-297	92.07	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497470 PE=3 SV=1	[more]
A0A6J1EJM8	6.7e-251	78.26	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434929 PE=3 SV=1	[more]
A0A6J1I6B8	3.7e-249	78.30	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470332 PE=3 SV=1	[more]
A0A6J1L6X0	3.1e-240	76.02	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499728 PE=3 SV=1	[more]

BLAST of CsaV3_2G017610 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G47550.1	8.9e-147	51.84	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G02810.1	1.1e-141	49.05	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02330.1	1.6e-140	48.80	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G10720.2	1.8e-115	43.21	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G04970.1	6.9e-115	43.56	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 22..171 e-value: 2.0E-4 score: 30.7
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 30..144 e-value: 3.6E-11 score: 43.6
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 238..552 e-value: 1.5E-114 score: 384.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 28..174 e-value: 5.0E-12 score: 48.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 30..174
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 244..538 e-value: 4.9E-122 score: 406.3
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 22..552
None	No IPR available	PANTHER	PTHR31707:SF275	PECTINESTERASE	coord: 22..552
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 31..176 e-value: 2.18955E-23 score: 94.4273
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 388..397
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 237..551

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_2G017610.1	CsaV3_2G017610.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity