CsaV3_2G016890 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_2G016890
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	EF hand calcium-binding family protein
Location	chr2: 14158583 .. 14160300 (+)
RNA-Seq Expression	CsaV3_2G016890
Synteny	CsaV3_2G016890

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCATTTTAATTCTTATACTTTCAATAGATATAAAAAATAAGTTGGTACAAATAGTTTATTAAAAGAGTAAATGATTTTTCCATAGACGTCCGAGACGGGTCTTTTATTTATATATTCTTTGACCTTACCTACATAATTAGATTGCTCCAAACTTAAGCACACTTAATTATAGAGTTCTTGTGATTGAGCCACCGTAGAAAAAATGTATATATACGTGATAGGTTTAAGTAGTAATATCTTTCAATTTTTTAAACATTTTTCAACCATACTTTCCTTTCCTATCTTCAAAATGCTGTTCAGGCAAATGTATTGATCTCGATTCATTCGTGTATCCTTTCTAAACTATGGAATAAAAAAGTATTATTTTTAAAAAATATAGGAAAAATAAATTTATATAGGAGGGATATAAATAGAGGGTTGATGGTTATACATTAATTGTGATTGGCATATAGGAAGAGATGTCAAGCTTATTGAAGTTATTCAGAAGCTGTTCTGGAAAGCGACCATCCAAAAACACTTCCTTTTCCATACCAATCAAAGAAAGGTCCAATTCTTCCTCACCCTCTTCAAAGCACTTCCGCCCAAACTTGGAGGAGATCAAATGGGTATGAACAATTCTTTTTTTGCATGAATTATTTCAAATCTTTTTATGAAAAAAACATTGAATTATTTCAAATGTTCATAAGCAATAAAGTTGCTGAGAAAGTTATGAACTGGAACTCTCTATAACAACATGCTGTGCTACGTACGTTCGTAGTAATGATCTCTTTTGGATTTAATAGGCGTTTGAGAAGTTTGACTCGAACAAGGATGGAAAAATCTCATTTGAGGAGTACAAGGAAGCTCACCGAGGCTTGGCTGGAAGCAAAGAGATCACAGACGCAGAGGCAGAGAAGTCATTCAAGCTTGTGGATGTAGATGGCGATGGATTCGTGGATTTGAAGGAGTTCGTGGAGCTGTACACGATGAGCAGCGGGGAAGTGAAGGTGGGCGACATCGAAAGTGCCTTCAAAGTTTATGATTCCAATGGAGATGGGAAGATAAGTGCAGAGGAGGTGATGGGGATCATGAAGATATTGGGGGAGAACACCACCTTGAAGGCTTGCAAGCAAATGGTGAAGGGTGTGGACATGGATGGGGATGGCTTTATTGATGTCCAAGAGTTTTCTAAGCTCATGGGTAAGTATTCGAGTAAGTTTCAAAATTAAAGAACCTAAAACTAAAACCTCGGACGCCCCGTATCCATTTTAATTTTTCTCGAGTGCAACGGTTGATCTCTAGCTTAATTTTCAAAAACAAAAACAAAAAAATAAAATGATTGGAAAACGAGAGCTACACATGTCTCTAGTTTTTAGACAAATGAACGTGGAAAACAAAAATTCTAAGCTATATTTGAAGTTTGTATCCAATAATTAGGAGGATAGTTATATTGTGACAATGTTGTTGATTAATGTGCGACTACTAGTAGTCATTATGCAACGTGTAGATTTGTGATGTAATTTTTGTTAAGTTGTCAACATCTTCAAACGATGAAGGGTTCAATATTGATATAGTGAATTGAAAATCTCTATTATATAAGAAAATTGATGTCAAGATTACTTTTATTGATATTTTCGTTAAGATTTACATTATTGTCAACATTCGTGAGTATAATATATAAGTGCGAGTTTTGTTGGGGCATTTTTAGATATAATATATATTGAACTAAAATATTTAT

mRNA sequence

ATGTCAAGCTTATTGAAGTTATTCAGAAGCTGTTCTGGAAAGCGACCATCCAAAAACACTTCCTTTTCCATACCAATCAAAGAAAGGTCCAATTCTTCCTCACCCTCTTCAAAGCACTTCCGCCCAAACTTGGAGGAGATCAAATGGGCGTTTGAGAAGTTTGACTCGAACAAGGATGGAAAAATCTCATTTGAGGAGTACAAGGAAGCTCACCGAGGCTTGGCTGGAAGCAAAGAGATCACAGACGCAGAGGCAGAGAAGTCATTCAAGCTTGTGGATGTAGATGGCGATGGATTCGTGGATTTGAAGGAGTTCGTGGAGCTGTACACGATGAGCAGCGGGGAAGTGAAGGTGGGCGACATCGAAAGTGCCTTCAAAGTTTATGATTCCAATGGAGATGGGAAGATAAGTGCAGAGGAGGTGATGGGGATCATGAAGATATTGGGGGAGAACACCACCTTGAAGGCTTGCAAGCAAATGGTGAAGGGTGTGGACATGGATGGGGATGGCTTTATTGATGTCCAAGAGTTTTCTAAGCTCATGGGTAAGTATTCGAGTAAGTTTCAAAATTAA

Coding sequence (CDS)

ATGTCAAGCTTATTGAAGTTATTCAGAAGCTGTTCTGGAAAGCGACCATCCAAAAACACTTCCTTTTCCATACCAATCAAAGAAAGGTCCAATTCTTCCTCACCCTCTTCAAAGCACTTCCGCCCAAACTTGGAGGAGATCAAATGGGCGTTTGAGAAGTTTGACTCGAACAAGGATGGAAAAATCTCATTTGAGGAGTACAAGGAAGCTCACCGAGGCTTGGCTGGAAGCAAAGAGATCACAGACGCAGAGGCAGAGAAGTCATTCAAGCTTGTGGATGTAGATGGCGATGGATTCGTGGATTTGAAGGAGTTCGTGGAGCTGTACACGATGAGCAGCGGGGAAGTGAAGGTGGGCGACATCGAAAGTGCCTTCAAAGTTTATGATTCCAATGGAGATGGGAAGATAAGTGCAGAGGAGGTGATGGGGATCATGAAGATATTGGGGGAGAACACCACCTTGAAGGCTTGCAAGCAAATGGTGAAGGGTGTGGACATGGATGGGGATGGCTTTATTGATGTCCAAGAGTTTTCTAAGCTCATGGGTAAGTATTCGAGTAAGTTTCAAAATTAA

Protein sequence

MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKLMGKYSSKFQN*

Homology

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BLAST of CsaV3_2G016890 vs. NCBI nr
Match: XP_004148036.1 (probable calcium-binding protein CML32 [Cucumis sativus] >KGN62012.1 hypothetical protein Csa_006079 [Cucumis sativus])

HSP 1 Score: 369.4 bits (947), Expect = 1.9e-98
Identity = 190/190 (100.00%), Postives = 190/190 (100.00%), Query Frame = 0


Query: 1   MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDG 60
           MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDG
Sbjct: 1   MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDG 60

Query: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 120
           KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD
Sbjct: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 120

Query: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 180
           IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL
Sbjct: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 180

Query: 181 MGKYSSKFQN 191
           MGKYSSKFQN
Sbjct: 181 MGKYSSKFQN 190

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BLAST of CsaV3_2G016890 vs. NCBI nr
Match: XP_008457835.1 (PREDICTED: probable calcium-binding protein CML32 [Cucumis melo] >KAA0045818.1 putative calcium-binding protein CML32 [Cucumis melo var. makuwa] >TYJ99463.1 putative calcium-binding protein CML32 [Cucumis melo var. makuwa])

HSP 1 Score: 350.9 bits (899), Expect = 6.9e-93
Identity = 181/189 (95.77%), Postives = 183/189 (96.83%), Query Frame = 0


Query: 1   MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDG 60
           M+SLLKLFRSCSGKRPSKNTSFSI IKERSNSSS SSKHFRPNLEEIKWAFEKFDSNKDG
Sbjct: 1   MTSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDG 60

Query: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 120
           KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVD DGDGFVDLKEFVELYTMSSGEVKVGD
Sbjct: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGD 120

Query: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 180
           IESAFKVYDSNGDGKISAEEVMGIMKILGENTTL ACKQMVKGVDMDGDGFID+QEFSKL
Sbjct: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKL 180

Query: 181 MGKYSSKFQ 190
           M  YSSKFQ
Sbjct: 181 MDTYSSKFQ 189

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BLAST of CsaV3_2G016890 vs. NCBI nr
Match: XP_038901754.1 (probable calcium-binding protein CML32 [Benincasa hispida])

HSP 1 Score: 301.6 bits (771), Expect = 4.8e-78
Identity = 154/188 (81.91%), Postives = 166/188 (88.30%), Query Frame = 0


Query: 3   SLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDGKI 62
           S+L LF SC+ KR  KN   S  IK+RSNSSS SSKHFRPNLEEIKWAFEKFDSNKDGKI
Sbjct: 7   SVLSLFTSCTRKRQPKNIPSSTQIKQRSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDGKI 66

Query: 63  SFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGDIE 122
           SFEEY+ AHRG+AGSKEI+D EA KSFK VDVDGDGFVDLKEF+ELYTMS GEV+VGDI+
Sbjct: 67  SFEEYQAAHRGMAGSKEISDEEAAKSFKFVDVDGDGFVDLKEFLELYTMSGGEVRVGDIK 126

Query: 123 SAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKLMG 182
           SAF+VYDSNGDGKISAEEVM IM  LGE TTLKACKQMVKGVD+DGDGFID+ EFSKLMG
Sbjct: 127 SAFQVYDSNGDGKISAEEVMAIMNTLGERTTLKACKQMVKGVDIDGDGFIDIDEFSKLMG 186

Query: 183 KYSSKFQN 191
            YSSKFQN
Sbjct: 187 TYSSKFQN 194

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BLAST of CsaV3_2G016890 vs. NCBI nr
Match: KAG6605248.1 (Calmodulin-like protein 30, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 270.8 bits (691), Expect = 9.1e-69
Identity = 144/188 (76.60%), Postives = 157/188 (83.51%), Query Frame = 0


Query: 3   SLLKLFRSCSGKRPSKNTSFSIPIKERSNS---SSPSSKHFRPNLEEIKWAFEKFDSNKD 62
           S+L +F SCS KR  K    S   K+RS S   SS SSKH R NLEEIKWAF+KFDSNKD
Sbjct: 7   SVLSIFTSCSPKRQLKQIPSSTQPKQRSKSISDSSASSKHVRVNLEEIKWAFDKFDSNKD 66

Query: 63  GKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVG 122
           GKISFEEYK AH  ++GSKEI+DAEA KSFKLVD DGDGFV+LKEFVELYTMS GEVK+G
Sbjct: 67  GKISFEEYKAAHGAMSGSKEISDAEAAKSFKLVDADGDGFVELKEFVELYTMSGGEVKMG 126

Query: 123 DIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSK 182
           DI+SAFKVYDSNGDGKISAEEVM IMKILGE+TTLKAC+QMVKGVD DGDGFID+ EFS 
Sbjct: 127 DIQSAFKVYDSNGDGKISAEEVMEIMKILGESTTLKACRQMVKGVDKDGDGFIDMDEFSS 186

Query: 183 LMGKYSSK 188
           LMG YS K
Sbjct: 187 LMGSYSFK 194

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BLAST of CsaV3_2G016890 vs. NCBI nr
Match: XP_022946935.1 (calmodulin-like protein 1 [Cucurbita moschata])

HSP 1 Score: 269.6 bits (688), Expect = 2.0e-68
Identity = 143/188 (76.06%), Postives = 156/188 (82.98%), Query Frame = 0


Query: 3   SLLKLFRSCSGKRPSKNTSFSIPIKERSNS---SSPSSKHFRPNLEEIKWAFEKFDSNKD 62
           S+L LF SCS KR  K    S   K+RS S   SS SSKH R NLEEIKW F+KFD+NKD
Sbjct: 7   SVLSLFTSCSPKRQLKQIPSSTQPKQRSKSISDSSASSKHVRVNLEEIKWVFDKFDTNKD 66

Query: 63  GKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVG 122
           GKISFEEYK AH  ++GSKEI+DAEA KSFKLVD DGDGFV+LKEFVELYTMS GEVK+G
Sbjct: 67  GKISFEEYKAAHGAMSGSKEISDAEAAKSFKLVDADGDGFVELKEFVELYTMSGGEVKMG 126

Query: 123 DIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSK 182
           DI+SAFKVYDSNGDGKISAEEVM IMKILGE+TTLKAC+QMVKGVD DGDGFID+ EFS 
Sbjct: 127 DIQSAFKVYDSNGDGKISAEEVMEIMKILGESTTLKACRQMVKGVDKDGDGFIDIDEFSS 186

Query: 183 LMGKYSSK 188
           LMG YS K
Sbjct: 187 LMGSYSFK 194

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BLAST of CsaV3_2G016890 vs. ExPASy Swiss-Prot
Match: Q9ZQE6 (Calmodulin-like protein 30 OS=Arabidopsis thaliana OX=3702 GN=CML30 PE=2 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 8.7e-30
Identity = 77/177 (43.50%), Postives = 113/177 (63.84%), Query Frame = 0


Query: 6   KLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHF-RPNLEEIKWAFEKFDSNKDGKISF 65
           KL ++   ++PS+  S     ++ S  SSP    F +P++ E++  F +FD +KDGKIS 
Sbjct: 12  KLSKNKMLRKPSRMFSRD---RQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLDKDGKISQ 71

Query: 66  EEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGDIESA 125
            EYK   R L   + I D    K FK VD+DGDGF+D +EF++ Y  S G ++  DI ++
Sbjct: 72  TEYKVVLRALGQERAIED--VPKIFKAVDLDGDGFIDFREFIDAYKRSGG-IRSSDIRNS 131

Query: 126 FKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKLM 182
           F  +D NGDGKISAEEVM ++  LGE  +L+ C +MV+ VD DGDG ++++EF K+M
Sbjct: 132 FWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKMM 182

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BLAST of CsaV3_2G016890 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 115.2 bits (287), Expect = 8.4e-25
Identity = 61/144 (42.36%), Postives = 93/144 (64.58%), Query Frame = 0


Query: 43  NLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDL 102
           ++++IK  F++FD N DGKIS +E KE  R L  S   +  E     K  D+DG+GF+DL
Sbjct: 14  SMDDIKKVFQRFDKNGDGKISVDELKEVIRAL--SPTASPEETVTMMKQFDLDGNGFIDL 73

Query: 103 KEFVELYTMSSG-----EVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKAC 162
            EFV L+ +  G        V D++ AF++YD +G+G+ISA+E+  +MK LGE  +++ C
Sbjct: 74  DEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDC 133

Query: 163 KQMVKGVDMDGDGFIDVQEFSKLM 182
           K+M+  VD+DGDG ++  EF K+M
Sbjct: 134 KKMISKVDIDGDGCVNFDEFKKMM 155

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BLAST of CsaV3_2G016890 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 4.2e-24
Identity = 60/148 (40.54%), Postives = 94/148 (63.51%), Query Frame = 0


Query: 37  SKHFRPNLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDG 96
           S++   ++E+IK  F++FD N DGKIS +E K+    L  S   +  E +   K  D+DG
Sbjct: 6   SRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGAL--SPNASQEETKAMMKEFDLDG 65

Query: 97  DGFVDLKEFVELYTM---SSGEVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTT 156
           +GF+DL EFV L+ +   SS    + D++ AF +YD + +G+ISA E+  +MK LGE  +
Sbjct: 66  NGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCS 125

Query: 157 LKACKQMVKGVDMDGDGFIDVQEFSKLM 182
           ++ C++M+  VD DGDG +D +EF K+M
Sbjct: 126 IQDCQRMINKVDSDGDGCVDFEEFKKMM 151

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BLAST of CsaV3_2G016890 vs. ExPASy Swiss-Prot
Match: Q9LF55 (Probable calcium-binding protein CML32 OS=Arabidopsis thaliana OX=3702 GN=CML32 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 4.6e-23
Identity = 63/143 (44.06%), Postives = 86/143 (60.14%), Query Frame = 0


Query: 51  FEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYT 110
           FE+ D NKDGKIS++E+ EA R  A S  IT  E +  F+ +DVDGD  +D+ E+     
Sbjct: 7   FERVDKNKDGKISWDEFAEAIR--AFSPSITSEEIDNMFREIDVDGDNQIDVAEYASC-L 66

Query: 111 MSSGEVKVGD----IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDM 170
           M  GE    D    ++ AF +YD +GDGKISA E+  ++K LGE  T+  C  MV+ VD 
Sbjct: 67  MLGGEGNKEDEDIVMKEAFDLYDIDGDGKISASEIHVVLKRLGEKQTIAECIAMVRAVDA 126

Query: 171 DGDGFIDVQEFSKLMGKYSSKFQ 190
           DGDGF+  +EF  +M   + K Q
Sbjct: 127 DGDGFVSFEEFKTMMSCNNKKLQ 146

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BLAST of CsaV3_2G016890 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 3.3e-21
Identity = 57/143 (39.86%), Postives = 90/143 (62.94%), Query Frame = 0


Query: 41  RPNLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFV 100
           +  + E++  F+KFD N DGKIS +E       L    E+ + E EK+   +D  GDG++
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSL--GHEVPEEELEKAITEIDRKGDGYI 91

Query: 101 DLKEFVELYT--MSSGEVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACK 160
           + +EFVEL T  M   +V + +++ AF VYD +G+G ISAEE+  +++ LG+  ++  C+
Sbjct: 92  NFEEFVELNTKGMDQNDV-LENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECR 151

Query: 161 QMVKGVDMDGDGFIDVQEFSKLM 182
           +M+ GVD DGDG ID +EF  +M
Sbjct: 152 KMIGGVDKDGDGTIDFEEFKIMM 171

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BLAST of CsaV3_2G016890 vs. ExPASy TrEMBL
Match: A0A0A0LMP3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G286450 PE=4 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 9.1e-99
Identity = 190/190 (100.00%), Postives = 190/190 (100.00%), Query Frame = 0


Query: 1   MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDG 60
           MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDG
Sbjct: 1   MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDG 60

Query: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 120
           KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD
Sbjct: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 120

Query: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 180
           IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL
Sbjct: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 180

Query: 181 MGKYSSKFQN 191
           MGKYSSKFQN
Sbjct: 181 MGKYSSKFQN 190

[Close]

BLAST of CsaV3_2G016890 vs. ExPASy TrEMBL
Match: A0A5D3BJJ3 (Putative calcium-binding protein CML32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G00260 PE=4 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 3.4e-93
Identity = 181/189 (95.77%), Postives = 183/189 (96.83%), Query Frame = 0


Query: 1   MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDG 60
           M+SLLKLFRSCSGKRPSKNTSFSI IKERSNSSS SSKHFRPNLEEIKWAFEKFDSNKDG
Sbjct: 1   MTSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDG 60

Query: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 120
           KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVD DGDGFVDLKEFVELYTMSSGEVKVGD
Sbjct: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGD 120

Query: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 180
           IESAFKVYDSNGDGKISAEEVMGIMKILGENTTL ACKQMVKGVDMDGDGFID+QEFSKL
Sbjct: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKL 180

Query: 181 MGKYSSKFQ 190
           M  YSSKFQ
Sbjct: 181 MDTYSSKFQ 189

[Close]

BLAST of CsaV3_2G016890 vs. ExPASy TrEMBL
Match: A0A1S3C6F1 (probable calcium-binding protein CML32 OS=Cucumis melo OX=3656 GN=LOC103497422 PE=4 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 3.4e-93
Identity = 181/189 (95.77%), Postives = 183/189 (96.83%), Query Frame = 0


Query: 1   MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDG 60
           M+SLLKLFRSCSGKRPSKNTSFSI IKERSNSSS SSKHFRPNLEEIKWAFEKFDSNKDG
Sbjct: 1   MTSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDG 60

Query: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 120
           KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVD DGDGFVDLKEFVELYTMSSGEVKVGD
Sbjct: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGD 120

Query: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 180
           IESAFKVYDSNGDGKISAEEVMGIMKILGENTTL ACKQMVKGVDMDGDGFID+QEFSKL
Sbjct: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKL 180

Query: 181 MGKYSSKFQ 190
           M  YSSKFQ
Sbjct: 181 MDTYSSKFQ 189

[Close]

BLAST of CsaV3_2G016890 vs. ExPASy TrEMBL
Match: A0A6J1G5E9 (calmodulin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111450966 PE=4 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 9.8e-69
Identity = 143/188 (76.06%), Postives = 156/188 (82.98%), Query Frame = 0


Query: 3   SLLKLFRSCSGKRPSKNTSFSIPIKERSNS---SSPSSKHFRPNLEEIKWAFEKFDSNKD 62
           S+L LF SCS KR  K    S   K+RS S   SS SSKH R NLEEIKW F+KFD+NKD
Sbjct: 7   SVLSLFTSCSPKRQLKQIPSSTQPKQRSKSISDSSASSKHVRVNLEEIKWVFDKFDTNKD 66

Query: 63  GKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVG 122
           GKISFEEYK AH  ++GSKEI+DAEA KSFKLVD DGDGFV+LKEFVELYTMS GEVK+G
Sbjct: 67  GKISFEEYKAAHGAMSGSKEISDAEAAKSFKLVDADGDGFVELKEFVELYTMSGGEVKMG 126

Query: 123 DIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSK 182
           DI+SAFKVYDSNGDGKISAEEVM IMKILGE+TTLKAC+QMVKGVD DGDGFID+ EFS 
Sbjct: 127 DIQSAFKVYDSNGDGKISAEEVMEIMKILGESTTLKACRQMVKGVDKDGDGFIDIDEFSS 186

Query: 183 LMGKYSSK 188
           LMG YS K
Sbjct: 187 LMGSYSFK 194

[Close]

BLAST of CsaV3_2G016890 vs. ExPASy TrEMBL
Match: A0A6J1L0C4 (calmodulin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111499885 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 3.2e-67
Identity = 139/188 (73.94%), Postives = 155/188 (82.45%), Query Frame = 0


Query: 3   SLLKLFRSCSGKRPSKNTSFSIPIKERSNS---SSPSSKHFRPNLEEIKWAFEKFDSNKD 62
           S+L LF SCS KR  K    S   K+R  S   SS SSKH + NLEEIKWAFEKFD+NKD
Sbjct: 7   SVLSLFTSCSPKRQLKQIPSSTQPKQRFKSISDSSASSKHVQVNLEEIKWAFEKFDTNKD 66

Query: 63  GKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVG 122
           GKISFEEYK AHR ++GS+EI+D EA KSFK VD DGDGFV+LKEFVELYTMS GEVK+G
Sbjct: 67  GKISFEEYKAAHRAMSGSQEISDVEAAKSFKFVDADGDGFVELKEFVELYTMSGGEVKMG 126

Query: 123 DIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSK 182
           D++SAFKVYDSNGDG+ISAEEVM IMKILGE+TTLKAC+QMVKGVD DGDGFID+ EFS 
Sbjct: 127 DVQSAFKVYDSNGDGRISAEEVMEIMKILGESTTLKACRQMVKGVDKDGDGFIDIDEFSS 186

Query: 183 LMGKYSSK 188
           LMG YS K
Sbjct: 187 LMGTYSFK 194

[Close]

BLAST of CsaV3_2G016890 vs. TAIR 10
Match: AT2G15680.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 131.7 bits (330), Expect = 6.2e-31
Identity = 77/177 (43.50%), Postives = 113/177 (63.84%), Query Frame = 0


Query: 6   KLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHF-RPNLEEIKWAFEKFDSNKDGKISF 65
           KL ++   ++PS+  S     ++ S  SSP    F +P++ E++  F +FD +KDGKIS 
Sbjct: 12  KLSKNKMLRKPSRMFSRD---RQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLDKDGKISQ 71

Query: 66  EEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGDIESA 125
            EYK   R L   + I D    K FK VD+DGDGF+D +EF++ Y  S G ++  DI ++
Sbjct: 72  TEYKVVLRALGQERAIED--VPKIFKAVDLDGDGFIDFREFIDAYKRSGG-IRSSDIRNS 131

Query: 126 FKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKLM 182
           F  +D NGDGKISAEEVM ++  LGE  +L+ C +MV+ VD DGDG ++++EF K+M
Sbjct: 132 FWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKMM 182

[Close]

BLAST of CsaV3_2G016890 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 115.2 bits (287), Expect = 6.0e-26
Identity = 61/144 (42.36%), Postives = 93/144 (64.58%), Query Frame = 0


Query: 43  NLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDL 102
           ++++IK  F++FD N DGKIS +E KE  R L  S   +  E     K  D+DG+GF+DL
Sbjct: 14  SMDDIKKVFQRFDKNGDGKISVDELKEVIRAL--SPTASPEETVTMMKQFDLDGNGFIDL 73

Query: 103 KEFVELYTMSSG-----EVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKAC 162
            EFV L+ +  G        V D++ AF++YD +G+G+ISA+E+  +MK LGE  +++ C
Sbjct: 74  DEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDC 133

Query: 163 KQMVKGVDMDGDGFIDVQEFSKLM 182
           K+M+  VD+DGDG ++  EF K+M
Sbjct: 134 KKMISKVDIDGDGCVNFDEFKKMM 155

[Close]

BLAST of CsaV3_2G016890 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 112.8 bits (281), Expect = 3.0e-25
Identity = 60/148 (40.54%), Postives = 94/148 (63.51%), Query Frame = 0


Query: 37  SKHFRPNLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDG 96
           S++   ++E+IK  F++FD N DGKIS +E K+    L  S   +  E +   K  D+DG
Sbjct: 6   SRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGAL--SPNASQEETKAMMKEFDLDG 65

Query: 97  DGFVDLKEFVELYTM---SSGEVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTT 156
           +GF+DL EFV L+ +   SS    + D++ AF +YD + +G+ISA E+  +MK LGE  +
Sbjct: 66  NGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCS 125

Query: 157 LKACKQMVKGVDMDGDGFIDVQEFSKLM 182
           ++ C++M+  VD DGDG +D +EF K+M
Sbjct: 126 IQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of CsaV3_2G016890 vs. TAIR 10
Match: AT5G17470.1 (EF hand calcium-binding protein family )

HSP 1 Score: 109.4 bits (272), Expect = 3.3e-24
Identity = 63/143 (44.06%), Postives = 86/143 (60.14%), Query Frame = 0


Query: 51  FEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYT 110
           FE+ D NKDGKIS++E+ EA R  A S  IT  E +  F+ +DVDGD  +D+ E+     
Sbjct: 7   FERVDKNKDGKISWDEFAEAIR--AFSPSITSEEIDNMFREIDVDGDNQIDVAEYASC-L 66

Query: 111 MSSGEVKVGD----IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDM 170
           M  GE    D    ++ AF +YD +GDGKISA E+  ++K LGE  T+  C  MV+ VD 
Sbjct: 67  MLGGEGNKEDEDIVMKEAFDLYDIDGDGKISASEIHVVLKRLGEKQTIAECIAMVRAVDA 126

Query: 171 DGDGFIDVQEFSKLMGKYSSKFQ 190
           DGDGF+  +EF  +M   + K Q
Sbjct: 127 DGDGFVSFEEFKTMMSCNNKKLQ 146

[Close]

BLAST of CsaV3_2G016890 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 103.2 bits (256), Expect = 2.3e-22
Identity = 57/143 (39.86%), Postives = 90/143 (62.94%), Query Frame = 0


Query: 41  RPNLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFV 100
           +  + E++  F+KFD N DGKIS +E       L    E+ + E EK+   +D  GDG++
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSL--GHEVPEEELEKAITEIDRKGDGYI 91

Query: 101 DLKEFVELYT--MSSGEVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACK 160
           + +EFVEL T  M   +V + +++ AF VYD +G+G ISAEE+  +++ LG+  ++  C+
Sbjct: 92  NFEEFVELNTKGMDQNDV-LENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECR 151

Query: 161 QMVKGVDMDGDGFIDVQEFSKLM 182
           +M+ GVD DGDG ID +EF  +M
Sbjct: 152 KMIGGVDKDGDGTIDFEEFKIMM 171

The following BLAST results are available for this feature:

BLAST of CsaV3_2G016890 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148036.1	1.9e-98	100.00	probable calcium-binding protein CML32 [Cucumis sativus] >KGN62012.1 hypothetica...	[more]
XP_008457835.1	6.9e-93	95.77	PREDICTED: probable calcium-binding protein CML32 [Cucumis melo] >KAA0045818.1 p...	[more]
XP_038901754.1	4.8e-78	81.91	probable calcium-binding protein CML32 [Benincasa hispida]	[more]
KAG6605248.1	9.1e-69	76.60	Calmodulin-like protein 30, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022946935.1	2.0e-68	76.06	calmodulin-like protein 1 [Cucurbita moschata]	[more]

BLAST of CsaV3_2G016890 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQE6	8.7e-30	43.50	Calmodulin-like protein 30 OS=Arabidopsis thaliana OX=3702 GN=CML30 PE=2 SV=1	[more]
P25070	8.4e-25	42.36	Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2	[more]
Q9C8Y1	4.2e-24	40.54	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]
Q9LF55	4.6e-23	44.06	Probable calcium-binding protein CML32 OS=Arabidopsis thaliana OX=3702 GN=CML32 ...	[more]
Q9FYK2	3.3e-21	39.86	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]

BLAST of CsaV3_2G016890 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LMP3	9.1e-99	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G286450 PE=4 SV=1	[more]
A0A5D3BJJ3	3.4e-93	95.77	Putative calcium-binding protein CML32 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3C6F1	3.4e-93	95.77	probable calcium-binding protein CML32 OS=Cucumis melo OX=3656 GN=LOC103497422 P...	[more]
A0A6J1G5E9	9.8e-69	76.06	calmodulin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111450966 PE=4 SV=...	[more]
A0A6J1L0C4	3.2e-67	73.94	calmodulin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111499885 PE=4 SV=1	[more]

BLAST of CsaV3_2G016890 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G15680.1	6.2e-31	43.50	Calcium-binding EF-hand family protein	[more]
AT5G37770.1	6.0e-26	42.36	EF hand calcium-binding protein family	[more]
AT1G66400.1	3.0e-25	40.54	calmodulin like 23	[more]
AT5G17470.1	3.3e-24	44.06	EF hand calcium-binding protein family	[more]
AT1G24620.1	2.3e-22	39.86	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 120..148 e-value: 1.8E-5 score: 34.2 coord: 84..112 e-value: 1.1E-4 score: 31.5 coord: 46..74 e-value: 1.7E-6 score: 37.5 coord: 156..184 e-value: 0.17 score: 21.0
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 121..181 e-value: 1.9E-9 score: 37.8 coord: 45..109 e-value: 4.9E-15 score: 55.7
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 80..115 score: 13.871811
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 152..187 score: 12.114401
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 116..151 score: 15.043417
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 42..77 score: 15.712908
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 120..181 e-value: 6.8898E-17 score: 69.4989
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 46..108 e-value: 5.25048E-14 score: 61.7949
None	No IPR available	GENE3D	1.10.238.10		coord: 14..114 e-value: 1.6E-19 score: 72.3
None	No IPR available	GENE3D	1.10.238.10		coord: 115..188 e-value: 7.6E-19 score: 70.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 9..40
None	No IPR available	PANTHER	PTHR10891:SF685	CALCIUM-BINDING PROTEIN CML34-RELATED	coord: 1..185
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 1..185
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 55..67
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 93..105
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 129..141
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 165..177
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 44..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_2G016890.1	CsaV3_2G016890.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding