CsaV3_2G016670 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_2G016670
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description(1->3)-beta-glucan endohydrolase
Locationchr2: 13887739 .. 13890557 (+)
RNA-Seq ExpressionCsaV3_2G016670
SyntenyCsaV3_2G016670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCAGAGAAAACATTCTCGAGTCCGATCATTCTCAAACTCCTACACCCAAAATCACGACGACCTCGAGCTAGCCATGACCGGCGCCACATTTGCCATTGCGGTCACATGGACCGTTCTAATAGTCGTTACAACAACGATCACCAGTGTGGTGCAGGCGCACTTGGGTGCGAATTGGGGTACAATGGCATCCCACCCATTGAACCCTAATATTGTGGTGAACTTGCTCGAGGACAATGGGATCAAGAAGGTGAAGTTGTTTGATTCTGATTCTTGGACTGTGAGTGCATTGGCTGGTTCCAAAATTGAAACCATTGTTGGCATTCCTAATGACCAACTTGAGAGCTTGGCTTCTGATTATAACCATGCTAAGGATTGGGTTAAGGAGAACGTCACCGCTCATATTTATGATGGAGGCGTCAATATAAGGTTCCTTTTAATTTCTCTTTTTATTAGTAGATTATTACAAATGTTTAAGTCTGTGTGTATTAGATTATTACAAAATGACTTAATAATCACGTTCAAATGTATATTAAGACAATATAAATAATTAGAATTGAAACATCACTAATTTTCAATAGTACAAATGAGGAGTAGAAAAATTGAACAACGAATCTCTTAGTCTTCAATACACACAAATTCTAATTGAGTTAAGTCTTTGTTGGGTTTGTCACTAAATCCCTAAACCTTCTTCATAGTCATGTTGTGTATTTTCTTAAAATTTGGTTTCTGGACAATTTTCTACTAAATATATATATAGAGGCCGCATTTAAACTATAACTTTGCTTATAGGTTATTTGTTATTTTAATTTACTAATTTATTTTTGTGGCATTTAAGTTATAATTAATTTAACCTGAAATATAAATGAAAAAAAAAGCCATAATTAATGAACTAATTTAACTATTTTTCCAAATGAAAACATCCACAGGTACATAGCTGTGGGCAATGAACCATTTTTAACAGCTTACAATGGCACCTACATAAAGCTAACATTTCCAGCCATGCAAAACATACAAAAAGCTCTCGATGAAGCCGGCTACAGCAAGAAGATAAAAGTAACATGCCCATTGAACGCCGACGTCTACGAGTCAGCCACAAACCAGCCCTCTGACGGGCAATTCCGATCAGATATCCTCGACGAGATGAAAGATATAGTCCGCTTCCTGAGTAGGAACGATGCTGCTTTCATGGTGAACATTTATCCCTTCCTTAGTCTCTACCTGAACTCTGACTTTCCCGTAGATTTTGCCTTCTTCGATGAAAACGGGAAGTCGATAAACGACAAGGGAAAGAAGTATACAAATGTATTCGATGCAAACTTCGACACGTTAGTTTGGTCGTTGAAGAAGATCGGTCTTGGAGATTTGAAGATCATAGTAGGGGAAGTCGGGTGGCCGACGGATGGTAATAAGTTTGCTACTGTGGAGTTGGCAAAGAGGTTTTATGATGGCTTGTTGAAGAAGTTGGCCAGCAAGAAGGGGACTCCCATGAGGCCAAATGAGAAGTTGGAAGTGTATTTGTTCGGCCTTTTAGATGAGGATTTGAAAAGCATTCAACCTGGCTTCTTTGAGCGTCATTGGGGTCTTTTCAGGTATGAATGATCGTAAATTCTATTTTGTTAGGGTTTGGAGAATAACATAAATTCTATTTTGAACGATCGTAAATTATATTTGATATCTGTTAAGTTTTTTATTTTTGAATCTTATATTTATTTTTTAGTTTTCATTCTTAATTTCTAGTTAAAGTAAATATTTGAGTTCTCAAGATGATTTTTAAGAAAAAGTAGGATTTTAAAGATTATTATTATATTTATCAACCTTCATATTTGATGTAGAAAAAAAAAATGCTCCGAATTTAGCACCACACAACACAACAAAGATCATTAAGTTGACTACGATCCATGAATAATGATAATAGTCAAACAAATTGATAAACAACGTCGATGATTTTCCATATTATTGAAAAACATTACAGGGTTATGATATTAAACAACAAGATTTCATCTCATAGTTAACCAATTTGTTTTAAGATGGAAGCTGACTTATAATGTTGTGTCAAAGTCCATCAAATTTAAATAGGCATTCAATAAAAAATAAAATCAGGATTTTATCCGAGAATATTAACCTAAAGAGACACTATCTCAAATGAGTATATATTGAGGATCCAACACAAAAAAAGAAAAAAAAAAACTAAAAGGATCTCACAGTTTTTACAAGATAGACGAGTTACTCTTCTAGTTGTCAATTAGTTCGAATTTTGAGACATAGTTGAAACATTTTACTCATTGTTTTACATGTGTTTCTTAATAGGTATGACGGAAAACCAAAGTTCTCGTTAGACTTAACAGGCAAAGGCAACGACAAGAGGCTAGTGGCAGCGAAGGGAGTTCAATACTTGGAACACAAATGGTGCGTGGTTAAGAACAGTGTTAAGGATTTAGGTACAATATCAAGTCAGATCGACTATGCTTGCTCCATGTCTGATTGCACAAGCTTAGGGTATGGGTCTTCTTGCAACAACCTGAACTCTAGAGGCAACATTTCTTATGCTTACAACATGTATTTCCAAATGCAAGATCAAAGTGTTGAAGCTTGTGTTTTTGGTGAATCTGCTGAAATTGTAACAAGAAATGCCTCTGTTGGCAGTTGCTTATTCCCCATCCAAATAGACAGCATCGGGCAACGGTTGACGCCAATGAACATTGTTGTTTTCACCGCCACCGTGTTGATTGGCCTAGTATTGAGCATGTATGTTTAAATGTATGACAAAGGTTTCAAAACATACCATTTAATTTAAATTAATAGACATAGTTATAACTACG

mRNA sequence

ATGACCGGCGCCACATTTGCCATTGCGGTCACATGGACCGTTCTAATAGTCGTTACAACAACGATCACCAGTGTGGTGCAGGCGCACTTGGGTGCGAATTGGGGTACAATGGCATCCCACCCATTGAACCCTAATATTGTGGTGAACTTGCTCGAGGACAATGGGATCAAGAAGGTGAAGTTGTTTGATTCTGATTCTTGGACTGTGAGTGCATTGGCTGGTTCCAAAATTGAAACCATTGTTGGCATTCCTAATGACCAACTTGAGAGCTTGGCTTCTGATTATAACCATGCTAAGGATTGGGTTAAGGAGAACGTCACCGCTCATATTTATGATGGAGGCGTCAATATAAGGTACATAGCTGTGGGCAATGAACCATTTTTAACAGCTTACAATGGCACCTACATAAAGCTAACATTTCCAGCCATGCAAAACATACAAAAAGCTCTCGATGAAGCCGGCTACAGCAAGAAGATAAAAGTAACATGCCCATTGAACGCCGACGTCTACGAGTCAGCCACAAACCAGCCCTCTGACGGGCAATTCCGATCAGATATCCTCGACGAGATGAAAGATATAGTCCGCTTCCTGAGTAGGAACGATGCTGCTTTCATGGTGAACATTTATCCCTTCCTTAGTCTCTACCTGAACTCTGACTTTCCCGTAGATTTTGCCTTCTTCGATGAAAACGGGAAGTCGATAAACGACAAGGGAAAGAAGTATACAAATGTATTCGATGCAAACTTCGACACGTTAGTTTGGTCGTTGAAGAAGATCGGTCTTGGAGATTTGAAGATCATAGTAGGGGAAGTCGGGTGGCCGACGGATGGTAATAAGTTTGCTACTGTGGAGTTGGCAAAGAGGTTTTATGATGGCTTGTTGAAGAAGTTGGCCAGCAAGAAGGGGACTCCCATGAGGCCAAATGAGAAGTTGGAAGTGTATTTGTTCGGCCTTTTAGATGAGGATTTGAAAAGCATTCAACCTGGCTTCTTTGAGCGTCATTGGGGTCTTTTCAGGTATGACGGAAAACCAAAGTTCTCGTTAGACTTAACAGGCAAAGGCAACGACAAGAGGCTAGTGGCAGCGAAGGGAGTTCAATACTTGGAACACAAATGGTGCGTGGTTAAGAACAGTGTTAAGGATTTAGGTACAATATCAAGTCAGATCGACTATGCTTGCTCCATGTCTGATTGCACAAGCTTAGGGTATGGGTCTTCTTGCAACAACCTGAACTCTAGAGGCAACATTTCTTATGCTTACAACATGTATTTCCAAATGCAAGATCAAAGTGTTGAAGCTTGTGTTTTTGGTGAATCTGCTGAAATTGTAACAAGAAATGCCTCTGTTGGCAGTTGCTTATTCCCCATCCAAATAGACAGCATCGGGCAACGGTTGACGCCAATGAACATTGTTGTTTTCACCGCCACCGTGTTGATTGGCCTAGTATTGAGCATGTATGTTTAA

Coding sequence (CDS)

ATGACCGGCGCCACATTTGCCATTGCGGTCACATGGACCGTTCTAATAGTCGTTACAACAACGATCACCAGTGTGGTGCAGGCGCACTTGGGTGCGAATTGGGGTACAATGGCATCCCACCCATTGAACCCTAATATTGTGGTGAACTTGCTCGAGGACAATGGGATCAAGAAGGTGAAGTTGTTTGATTCTGATTCTTGGACTGTGAGTGCATTGGCTGGTTCCAAAATTGAAACCATTGTTGGCATTCCTAATGACCAACTTGAGAGCTTGGCTTCTGATTATAACCATGCTAAGGATTGGGTTAAGGAGAACGTCACCGCTCATATTTATGATGGAGGCGTCAATATAAGGTACATAGCTGTGGGCAATGAACCATTTTTAACAGCTTACAATGGCACCTACATAAAGCTAACATTTCCAGCCATGCAAAACATACAAAAAGCTCTCGATGAAGCCGGCTACAGCAAGAAGATAAAAGTAACATGCCCATTGAACGCCGACGTCTACGAGTCAGCCACAAACCAGCCCTCTGACGGGCAATTCCGATCAGATATCCTCGACGAGATGAAAGATATAGTCCGCTTCCTGAGTAGGAACGATGCTGCTTTCATGGTGAACATTTATCCCTTCCTTAGTCTCTACCTGAACTCTGACTTTCCCGTAGATTTTGCCTTCTTCGATGAAAACGGGAAGTCGATAAACGACAAGGGAAAGAAGTATACAAATGTATTCGATGCAAACTTCGACACGTTAGTTTGGTCGTTGAAGAAGATCGGTCTTGGAGATTTGAAGATCATAGTAGGGGAAGTCGGGTGGCCGACGGATGGTAATAAGTTTGCTACTGTGGAGTTGGCAAAGAGGTTTTATGATGGCTTGTTGAAGAAGTTGGCCAGCAAGAAGGGGACTCCCATGAGGCCAAATGAGAAGTTGGAAGTGTATTTGTTCGGCCTTTTAGATGAGGATTTGAAAAGCATTCAACCTGGCTTCTTTGAGCGTCATTGGGGTCTTTTCAGGTATGACGGAAAACCAAAGTTCTCGTTAGACTTAACAGGCAAAGGCAACGACAAGAGGCTAGTGGCAGCGAAGGGAGTTCAATACTTGGAACACAAATGGTGCGTGGTTAAGAACAGTGTTAAGGATTTAGGTACAATATCAAGTCAGATCGACTATGCTTGCTCCATGTCTGATTGCACAAGCTTAGGGTATGGGTCTTCTTGCAACAACCTGAACTCTAGAGGCAACATTTCTTATGCTTACAACATGTATTTCCAAATGCAAGATCAAAGTGTTGAAGCTTGTGTTTTTGGTGAATCTGCTGAAATTGTAACAAGAAATGCCTCTGTTGGCAGTTGCTTATTCCCCATCCAAATAGACAGCATCGGGCAACGGTTGACGCCAATGAACATTGTTGTTTTCACCGCCACCGTGTTGATTGGCCTAGTATTGAGCATGTATGTTTAA

Protein sequence

MTGATFAIAVTWTVLIVVTTTITSVVQAHLGANWGTMASHPLNPNIVVNLLEDNGIKKVKLFDSDSWTVSALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYIAVGNEPFLTAYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDGQFRSDILDEMKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKKYTNVFDANFDTLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASKKGTPMRPNEKLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLVAAKGVQYLEHKWCVVKNSVKDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAYNMYFQMQDQSVEACVFGESAEIVTRNASVGSCLFPIQIDSIGQRLTPMNIVVFTATVLIGLVLSMYV*
Homology
BLAST of CsaV3_2G016670 vs. NCBI nr
Match: XP_004149714.1 (glucan endo-1,3-beta-glucosidase 8 [Cucumis sativus] >KGN61959.1 hypothetical protein Csa_006377 [Cucumis sativus])

HSP 1 Score: 979.5 bits (2531), Expect = 1.0e-281
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0

Query: 1   MTGATFAIAVTWTVLIVVTTTITSVVQAHLGANWGTMASHPLNPNIVVNLLEDNGIKKVK 60
           MTGATFAIAVTWTVLIVVTTTITSVVQAHLGANWGTMASHPLNPNIVVNLLEDNGIKKVK
Sbjct: 1   MTGATFAIAVTWTVLIVVTTTITSVVQAHLGANWGTMASHPLNPNIVVNLLEDNGIKKVK 60

Query: 61  LFDSDSWTVSALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYI 120
           LFDSDSWTVSALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYI
Sbjct: 61  LFDSDSWTVSALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYI 120

Query: 121 AVGNEPFLTAYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDG 180
           AVGNEPFLTAYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDG
Sbjct: 121 AVGNEPFLTAYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDG 180

Query: 181 QFRSDILDEMKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKK 240
           QFRSDILDEMKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKK
Sbjct: 181 QFRSDILDEMKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKK 240

Query: 241 YTNVFDANFDTLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASK 300
           YTNVFDANFDTLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASK
Sbjct: 241 YTNVFDANFDTLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASK 300

Query: 301 KGTPMRPNEKLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLV 360
           KGTPMRPNEKLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLV
Sbjct: 301 KGTPMRPNEKLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLV 360

Query: 361 AAKGVQYLEHKWCVVKNSVKDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAY 420
           AAKGVQYLEHKWCVVKNSVKDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAY
Sbjct: 361 AAKGVQYLEHKWCVVKNSVKDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAY 420

Query: 421 NMYFQMQDQSVEACVFGESAEIVTRNASVGSCLFPIQIDSIGQRLTPMNIVVFTATVLIG 480
           NMYFQMQDQSVEACVFGESAEIVTRNASVGSCLFPIQIDSIGQRLTPMNIVVFTATVLIG
Sbjct: 421 NMYFQMQDQSVEACVFGESAEIVTRNASVGSCLFPIQIDSIGQRLTPMNIVVFTATVLIG 480

Query: 481 LVLSMYV 488
           LVLSMYV
Sbjct: 481 LVLSMYV 487

BLAST of CsaV3_2G016670 vs. NCBI nr
Match: XP_008457953.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 8-like [Cucumis melo] >KAA0045792.1 glucan endo-1,3-beta-glucosidase 8-like [Cucumis melo var. makuwa] >TYJ99489.1 glucan endo-1,3-beta-glucosidase 8-like [Cucumis melo var. makuwa])

HSP 1 Score: 891.0 bits (2301), Expect = 4.7e-255
Identity = 432/487 (88.71%), Postives = 463/487 (95.07%), Query Frame = 0

Query: 1   MTGATFAIAVTWTVLIVVTTTITSVVQAHLGANWGTMASHPLNPNIVVNLLEDNGIKKVK 60
           M  ATFAIAVTWTVLIVVTTT+TSVVQA+LG NWG+MASHPLNPNIVVNLL+DNGIKKVK
Sbjct: 1   MASATFAIAVTWTVLIVVTTTVTSVVQANLGVNWGSMASHPLNPNIVVNLLKDNGIKKVK 60

Query: 61  LFDSDSWTVSALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYI 120
           LFDSDSWTV+AL+GSKIETIVGIPNDQLESLA++YNHAKDWVKEN+TAHIYDGGVNIRY+
Sbjct: 61  LFDSDSWTVNALSGSKIETIVGIPNDQLESLATNYNHAKDWVKENITAHIYDGGVNIRYV 120

Query: 121 AVGNEPFLTAYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDG 180
           AVGNEPFLTAYNGTY+KLTFPAMQNIQKAL+EAGY+KKIKVTCPLNADVYESA+N PSDG
Sbjct: 121 AVGNEPFLTAYNGTYVKLTFPAMQNIQKALNEAGYNKKIKVTCPLNADVYESASNHPSDG 180

Query: 181 QFRSDILDEMKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKK 240
           QFRSDILDEMK+IV+FL RN+AAFMVNIYPFLSLYLN DFPVDFAF DENG+SINDKGK 
Sbjct: 181 QFRSDILDEMKEIVQFLDRNNAAFMVNIYPFLSLYLNPDFPVDFAFMDENGRSINDKGKT 240

Query: 241 YTNVFDANFDTLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASK 300
           YTNV DAN+DTLVWSLKKIG GD+KIIVGEVGWPTDGNKFATV LAKRFYDGLLKKLASK
Sbjct: 241 YTNVLDANYDTLVWSLKKIGHGDMKIIVGEVGWPTDGNKFATVGLAKRFYDGLLKKLASK 300

Query: 301 KGTPMRPNEKLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLV 360
           KGTPMRPNEKLEVYLFGL DED KSIQPGFFERHWGLFRYDGKPKF +D TGK NDK LV
Sbjct: 301 KGTPMRPNEKLEVYLFGLFDEDTKSIQPGFFERHWGLFRYDGKPKFPIDFTGKDNDKMLV 360

Query: 361 AAKGVQYLEHKWCVVKNSVKDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAY 420
           AAKGVQYLEHKWCVVKNSVKDL TISSQ+DYACSMSDCT+LGYGSSCNNLN++GNISYAY
Sbjct: 361 AAKGVQYLEHKWCVVKNSVKDLRTISSQVDYACSMSDCTTLGYGSSCNNLNAKGNISYAY 420

Query: 421 NMYFQMQDQSVEACVFGESAEIVTRNASVGSCLFPIQIDSIGQRLTPMNIVVFTATVLIG 480
           NMYFQMQDQSVEACVFG+SAEIVT+NASVG+CLFPIQI S+GQRL  MNIVVF  TVL+ 
Sbjct: 421 NMYFQMQDQSVEACVFGDSAEIVTKNASVGNCLFPIQIVSVGQRLMTMNIVVFVTTVLVS 480

Query: 481 LVLSMYV 488
           L+LSMYV
Sbjct: 481 LMLSMYV 487

BLAST of CsaV3_2G016670 vs. NCBI nr
Match: XP_038902854.1 (glucan endo-1,3-beta-glucosidase 8-like [Benincasa hispida])

HSP 1 Score: 830.5 bits (2144), Expect = 7.6e-237
Identity = 407/487 (83.57%), Postives = 439/487 (90.14%), Query Frame = 0

Query: 1   MTGATFAIAVTWTVLIVVTTTITSVVQAHLGANWGTMASHPLNPNIVVNLLEDNGIKKVK 60
           M  AT AIAV W  L+VVT T+  VV+A+LG NWGTMASHPLNPNIVVNLL+DNGIKKVK
Sbjct: 1   MAKATLAIAVVWAALMVVTATVIGVVEANLGVNWGTMASHPLNPNIVVNLLKDNGIKKVK 60

Query: 61  LFDSDSWTVSALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYI 120
           LFDSDSWTVSALAGSKIETIV IPNDQLESLASDYN AKDWVKEN+T HIY+GGV+IRY+
Sbjct: 61  LFDSDSWTVSALAGSKIETIVAIPNDQLESLASDYNLAKDWVKENITTHIYEGGVDIRYV 120

Query: 121 AVGNEPFLTAYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDG 180
           AVGNEPFLTA+NG+Y+KLTFPAM+NIQKAL+EAG+ KKIK T PLNADVYESA+NQPSDG
Sbjct: 121 AVGNEPFLTAFNGSYVKLTFPAMENIQKALNEAGHGKKIKTTTPLNADVYESASNQPSDG 180

Query: 181 QFRSDILDEMKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKK 240
           QFRSDI D +K+IV FLS NDA FMVNIYPFLSLY N DFPVDFAF DE GKSINDKGK 
Sbjct: 181 QFRSDIHDTIKEIVNFLSHNDAPFMVNIYPFLSLYQNPDFPVDFAFLDEIGKSINDKGKT 240

Query: 241 YTNVFDANFDTLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASK 300
           YTNVFDAN+DTLVWSLKKIG G+LKIIVGEVGWPT+ NKFA VELAKRFYDGL KKLASK
Sbjct: 241 YTNVFDANYDTLVWSLKKIGAGNLKIIVGEVGWPTEANKFANVELAKRFYDGLFKKLASK 300

Query: 301 KGTPMRPNEKLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLV 360
           KGTPMRPNEKLEVYLFGL DED+KSI PGFFERHWG+FRYDGKPKF +D TGKGND+  V
Sbjct: 301 KGTPMRPNEKLEVYLFGLFDEDMKSILPGFFERHWGIFRYDGKPKFPMDFTGKGNDEMPV 360

Query: 361 AAKGVQYLEHKWCVVKNSVKDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAY 420
           AAKGVQYLEHKWCVVKNSVKDL TI+SQIDYACSMSDCTSL YGSSCNNLN RGN+S+AY
Sbjct: 361 AAKGVQYLEHKWCVVKNSVKDLRTIASQIDYACSMSDCTSLVYGSSCNNLNPRGNVSFAY 420

Query: 421 NMYFQMQDQSVEACVFGESAEIVTRNASVGSCLFPIQIDSIGQRLTPMNIVVFTATVLIG 480
           NMYFQMQDQSVEACVF  SAEIVT+NASVG+CLFPIQI S GQRLT MNI  F ATVL+ 
Sbjct: 421 NMYFQMQDQSVEACVFDGSAEIVTKNASVGNCLFPIQIVSTGQRLTTMNIADFVATVLVA 480

Query: 481 LVLSMYV 488
           LVL+MYV
Sbjct: 481 LVLNMYV 487

BLAST of CsaV3_2G016670 vs. NCBI nr
Match: KAG7011052.1 (Glucan endo-1,3-beta-glucosidase 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 751.5 bits (1939), Expect = 4.5e-213
Identity = 364/483 (75.36%), Postives = 421/483 (87.16%), Query Frame = 0

Query: 5   TFAIAVTWTVLIVVTTTITSVVQAHL--GANWGTMASHPLNPNIVVNLLEDNGIKKVKLF 64
           +FAIAVTW  L+ +TT    VV+A+L  G NWGT+ASHP+NP+IVVNLL+DNGIKKVKLF
Sbjct: 6   SFAIAVTWATLMALTT----VVEANLDVGVNWGTIASHPINPSIVVNLLKDNGIKKVKLF 65

Query: 65  DSDSWTVSALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYIAV 124
           DSDSW + ALAGSKIET+V IPNDQLE  A+DY+ AKDWVKENVT +IYDGGV+IRY+AV
Sbjct: 66  DSDSWIMGALAGSKIETVVAIPNDQLEKFANDYDLAKDWVKENVTTYIYDGGVDIRYVAV 125

Query: 125 GNEPFLTAYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDGQF 184
           GNEPFLT +NG+YIKLTFPAMQN+QKAL++AG+  KIKVT PLNADVYES +NQPSDG+F
Sbjct: 126 GNEPFLTGFNGSYIKLTFPAMQNVQKALNDAGHGDKIKVTTPLNADVYESPSNQPSDGRF 185

Query: 185 RSDILDEMKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKKYT 244
           RSDI D MK+IV FLS N+A FMVNIYPFLSLY N DFP+DFAF D +GKSINDKGK YT
Sbjct: 186 RSDIHDTMKNIVHFLSSNNAPFMVNIYPFLSLYQNPDFPIDFAFLDSSGKSINDKGKTYT 245

Query: 245 NVFDANFDTLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASKKG 304
           NVFDAN+DTLVWSLKKIG GDLKI+VGEVGWPTD NK+A +ELAKRFYDGL K LASKKG
Sbjct: 246 NVFDANYDTLVWSLKKIGEGDLKIVVGEVGWPTDANKYANLELAKRFYDGLFKNLASKKG 305

Query: 305 TPMRPNEKLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLVAA 364
           TPMRP+EKLE+YLF L DED+KS+ PGFFERHWG+FRYDGKPKF +DL G GNDK  VAA
Sbjct: 306 TPMRPDEKLEIYLFSLFDEDMKSVLPGFFERHWGIFRYDGKPKFPMDLAGNGNDKMPVAA 365

Query: 365 KGVQYLEHKWCVVKNSVKDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAYNM 424
           KGVQYLE KWCV+KNS++DL +  SQIDYACS+SDCT+L +GSSCN+LN+RGN S+AYNM
Sbjct: 366 KGVQYLEKKWCVIKNSIEDLTSEVSQIDYACSLSDCTTLVFGSSCNDLNARGNFSFAYNM 425

Query: 425 YFQMQDQSVEACVFGESAEIVTRNASVGSCLFPIQIDSIGQRLTPMNIVVFTATVLIGLV 484
           YFQMQDQSVEAC FG SAEIVT+NASVG+CLFPIQI S GQ+ T MN+V F +TV + ++
Sbjct: 426 YFQMQDQSVEACDFGGSAEIVTKNASVGTCLFPIQIVSAGQK-TMMNVVPFVSTVFVTIL 483

Query: 485 LSM 486
            S+
Sbjct: 486 FSV 483

BLAST of CsaV3_2G016670 vs. NCBI nr
Match: XP_022927955.1 (glucan endo-1,3-beta-glucosidase 8-like [Cucurbita moschata])

HSP 1 Score: 750.0 bits (1935), Expect = 1.3e-212
Identity = 363/483 (75.16%), Postives = 420/483 (86.96%), Query Frame = 0

Query: 5   TFAIAVTWTVLIVVTTTITSVVQAHL--GANWGTMASHPLNPNIVVNLLEDNGIKKVKLF 64
           +FAIAVTW  L+ +TT    VV+A+L  G NWGT+ASHP+NP+IVVNLL+DNGIKKVKLF
Sbjct: 6   SFAIAVTWATLMALTT----VVEANLDVGVNWGTIASHPINPSIVVNLLKDNGIKKVKLF 65

Query: 65  DSDSWTVSALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYIAV 124
           DSDSW + ALAGSKIET+V IPNDQLE  A+DY+ AKDWVKENVT +IYDGGV+IRY+AV
Sbjct: 66  DSDSWIMGALAGSKIETVVAIPNDQLEKFANDYDLAKDWVKENVTTYIYDGGVDIRYVAV 125

Query: 125 GNEPFLTAYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDGQF 184
           GNEPFLT +NG+YIKLTFPAMQN+QKAL++AG+  KIKVT PLNADVYES +NQPSDG+F
Sbjct: 126 GNEPFLTGFNGSYIKLTFPAMQNVQKALNDAGHGDKIKVTTPLNADVYESPSNQPSDGRF 185

Query: 185 RSDILDEMKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKKYT 244
           RSDI D MK+IV FLS N+A FMVNIYPFLSLY N DFP+DFAF D +GKSINDKGK YT
Sbjct: 186 RSDIHDTMKNIVHFLSSNNAPFMVNIYPFLSLYQNPDFPIDFAFLDSSGKSINDKGKTYT 245

Query: 245 NVFDANFDTLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASKKG 304
           NVFDAN+DTLVWSLKKIG GDLKI+VGEVGWPTD NK+A +ELAKRFYDGL K LASKKG
Sbjct: 246 NVFDANYDTLVWSLKKIGEGDLKIVVGEVGWPTDANKYANLELAKRFYDGLFKNLASKKG 305

Query: 305 TPMRPNEKLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLVAA 364
           TPMRP+EKLE+YLF L DED+KS+ PGFFERHWG+FRYDGKPKF +DL G GNDK  VAA
Sbjct: 306 TPMRPDEKLEIYLFSLFDEDMKSVLPGFFERHWGIFRYDGKPKFPMDLAGNGNDKMPVAA 365

Query: 365 KGVQYLEHKWCVVKNSVKDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAYNM 424
           KGVQYLE KWCV+KNS++DL +  SQIDYACS+SDCT+L +GSSCN+LN+RGN S+AYNM
Sbjct: 366 KGVQYLEKKWCVIKNSIEDLTSEVSQIDYACSLSDCTTLVFGSSCNDLNARGNFSFAYNM 425

Query: 425 YFQMQDQSVEACVFGESAEIVTRNASVGSCLFPIQIDSIGQRLTPMNIVVFTATVLIGLV 484
           YFQMQDQSVEAC FG SAEIVT+NASVG+CLFPIQI S GQ+ T MN+V F  T+ + ++
Sbjct: 426 YFQMQDQSVEACDFGGSAEIVTKNASVGTCLFPIQIVSAGQK-TMMNVVPFVFTIFVTIL 483

Query: 485 LSM 486
            S+
Sbjct: 486 FSV 483

BLAST of CsaV3_2G016670 vs. ExPASy Swiss-Prot
Match: Q6NKW9 (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 PE=2 SV=2)

HSP 1 Score: 533.9 bits (1374), Expect = 1.9e-150
Identity = 257/431 (59.63%), Postives = 323/431 (74.94%), Query Frame = 0

Query: 30  LGANWGTMASHPLNPNIVVNLLEDNGIKKVKLFDSDSWTVSALAGSKIETIVGIPNDQLE 89
           LG NWGTMA+H L P  VV +L+DN I KVKLFD+D  T+ ALAGS +E +V IPNDQL+
Sbjct: 25  LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVMVAIPNDQLK 84

Query: 90  SLASDYNHAKDWVKENVTAHIYDGGVNIRYIAVGNEPFLTAYNGTYIKLTFPAMQNIQKA 149
            + S Y+ AKDWV++NVT + +DGGVNI ++AVGNEPFL +YNG++I LTFPA+ NIQ A
Sbjct: 85  VMTS-YDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFPALANIQNA 144

Query: 150 LDEAGYSKKIKVTCPLNADVYESATNQ--PSDGQFRSDILDEMKDIVRFLSRNDAAFMVN 209
           L+EAG    +K T PLNADVY+S  +   PS G+FR DI+ +M  IV FL +N+A   +N
Sbjct: 145 LNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGKNNAPITIN 204

Query: 210 IYPFLSLYLNSDFPVDFAFFDENGKSINDKGKKYTNVFDANFDTLVWSLKKIGLGDLKII 269
           IYPFLSLY N DFP+++AFFD   + IND G  YTNVFDANFDTLV SLK +G GD+ II
Sbjct: 205 IYPFLSLYGNDDFPLNYAFFD-GAEPINDNGIDYTNVFDANFDTLVSSLKAVGHGDMPII 264

Query: 270 VGEVGWPTDGNKFATVELAKRFYDGLLKKLASKKGTPMRPNEKLEVYLFGLLDEDLKSIQ 329
           VGEVGWPT+G+K A    A RFY+GLL +L + KGTP+RP   +EVYLFGLLDED KSI 
Sbjct: 265 VGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPT-YIEVYLFGLLDEDAKSIA 324

Query: 330 PGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLVAAKGVQYLEHKWCVVKNSVKDLGTISS 389
           PG FERHWG+F++DG+PKF +DL+G+G  K L+ A+ V YL +KWC      KDL  +++
Sbjct: 325 PGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAKDLTKLAA 384

Query: 390 QIDYACSMSDCTSLGYGSSCNNLNSRGNISYAYNMYFQMQDQSVEACVFGESAEIVTRNA 449
            IDYAC+ SDCT+LGYGSSCN L++ GN SYA+NM+FQ+++Q   AC F   A I T+N 
Sbjct: 385 NIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGLATITTQNI 444

Query: 450 SVGSCLFPIQI 459
           S G C FPIQI
Sbjct: 445 SQGQCNFPIQI 452

BLAST of CsaV3_2G016670 vs. ExPASy Swiss-Prot
Match: Q93Z08 (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 PE=2 SV=2)

HSP 1 Score: 501.9 bits (1291), Expect = 8.1e-141
Identity = 240/448 (53.57%), Postives = 325/448 (72.54%), Query Frame = 0

Query: 13  TVLIVVTTTITSVVQAHLGANWGTMASHPLNPNIVVNLLEDNGIKKVKLFDSDSWTVSAL 72
           +VL+++   +     + +GANWGT ASHPL P+IVV +L +NGI+KVKLFD++  T+ AL
Sbjct: 5   SVLLLLAVALLCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAEYDTLRAL 64

Query: 73  AGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYIAVGNEPFLTAYN 132
             S IE +VGIPN+ L +LAS    A+ WV +NV+ HI    VNIRY+AVGNEPFL+ YN
Sbjct: 65  GKSGIEVMVGIPNEMLATLASSLKAAEKWVAKNVSTHISTDNVNIRYVAVGNEPFLSTYN 124

Query: 133 GTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDGQFRSDILDEMKD 192
           G+Y+  TFPA++NIQ A+ +AG   ++KVTCPLNADVY+S+T  PS G FR++I D M  
Sbjct: 125 GSYLSTTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDSSTTFPSGGDFRANIRDLMIT 184

Query: 193 IVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKKYTNVFDANFDTL 252
           IV+FLS N   F VNIYP++SLY N DFPVD+AFFD N + +ND G  Y N+FDAN+DTL
Sbjct: 185 IVKFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLNDGGTFYYNMFDANYDTL 244

Query: 253 VWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASKKGTPMRPNEKLE 312
           V +L+K G G++ II+GE+GWPTDG+  A ++ AK+F  G +  ++  KGTP RP   ++
Sbjct: 245 VHALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTPRRPG-PID 304

Query: 313 VYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLVAAKGVQYLEHKW 372
            YLF L+DED KS+QPG+FERHWG+F +DG PK++L+L G  N   L+ AKGV+YLE KW
Sbjct: 305 AYLFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNL-GTTNTGALIQAKGVRYLERKW 364

Query: 373 CVVKNSVK-DLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAYNMYFQMQDQSV 432
           CV+K +V+ D   ++  + YACS+ DCTSLG G+SC NL+ + NISYA+N Y+Q+QDQ  
Sbjct: 365 CVMKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYYQIQDQLD 424

Query: 433 EACVFGESAEIVTRNASVGSCLFPIQID 460
            AC F   +E+   + S G+C FPI I+
Sbjct: 425 TACKFPNISEVTKTDPSTGTCRFPIMIE 450

BLAST of CsaV3_2G016670 vs. ExPASy Swiss-Prot
Match: Q9M088 (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 PE=2 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 3.0e-135
Identity = 249/480 (51.88%), Postives = 328/480 (68.33%), Query Frame = 0

Query: 12  WTVLIVVTTTITSVV--QAHLGANWGTMASHPLNPNIVVNLLEDNGIKKVKLFDSDSWTV 71
           + V  V+T    S++     +G NWG+ A HPL P  VV LL +NGI+KVKLF++DS  +
Sbjct: 7   FAVFFVITLLYASLLIEVEGIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAIL 66

Query: 72  SALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYIAVGNEPFLT 131
            AL+ + I+ +VGIPND L  LA     A+ WV +NV+AH+   GV+IRY+AVGNEPFL 
Sbjct: 67  KALSRTGIQVMVGIPNDLLAPLAGSVAAAERWVSQNVSAHVSSNGVDIRYVAVGNEPFLK 126

Query: 132 AYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDGQFRSDILDE 191
           A+NGT+  +T PA+QNIQ A+ +AG + ++KVT PLNADVY+SA+N PSDG FR +I D 
Sbjct: 127 AFNGTFEGITLPALQNIQSAIIKAGLATQVKVTVPLNADVYQSASNLPSDGDFRPEIRDL 186

Query: 192 MKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKKYTNVFDANF 251
           M +IV+FLS N A F +NIYPF+SLY + +FPV+FAFFD  G  IND G+ Y NV DAN+
Sbjct: 187 MLNIVKFLSDNGAPFTINIYPFISLYNDPNFPVEFAFFDGTGTPINDNGRIYDNVLDANY 246

Query: 252 DTLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASKKGTPMRPNE 311
           DTLVWSL+K G G+L IIVGEVGWPTDG+K A +  A+R+  G + +  + KGTPMRP  
Sbjct: 247 DTLVWSLQKNGFGNLTIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRPG- 306

Query: 312 KLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLVAAKGVQYLE 371
            ++ YLFGL+DED KSIQPG FERHWG+F  DG+PK+ L L G GN   L+ AK V YL 
Sbjct: 307 AMDAYLFGLIDEDAKSIQPGNFERHWGIFYIDGQPKYQLSL-GSGNG--LIPAKDVHYLA 366

Query: 372 HKWCVV-KNSVKDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAYNMYFQMQD 431
            KWC++  N+      +   + YAC  +DCTSLGYGSSC NLN   N+SYA+N Y+Q+ +
Sbjct: 367 KKWCILAPNANLQDPQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSN 426

Query: 432 QSVEACVFGESAEIVTRNASVGSCLFPIQIDS-IGQRLTPMNIVVFTATVLIGLVLSMYV 488
           Q   AC F   + + TR+ SVGSC F I I S      + M  +  +  VL+ L + +Y+
Sbjct: 427 QLDSACKFPGLSIVSTRDPSVGSCKFKIMIKSEDASEASAMMPITRSTAVLLLLSICLYI 482

BLAST of CsaV3_2G016670 vs. ExPASy Swiss-Prot
Match: Q9FGH4 (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 PE=2 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 1.2e-96
Identity = 190/450 (42.22%), Postives = 271/450 (60.22%), Query Frame = 0

Query: 14  VLIVVTT--TITSVVQAHLGANWGTMASHPLNPNIVVNLLEDNGIKKVKLFDSDSWTVSA 73
           +L+ VT   ++T      +G NWGT ASHPL P+ VV LL+ NGI KVKLFD+D   + A
Sbjct: 8   LLLAVTAGLSLTGTTVRAVGINWGTEASHPLPPSKVVELLKSNGIVKVKLFDADPKVLRA 67

Query: 74  LAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGG-VNIRYIAVGNEPFLTA 133
           L+GS I   +GI N  L+SL +    A+ WV +NVT +   G  V I Y+AVG EPFL +
Sbjct: 68  LSGSNIGVTIGIQNSMLKSLNASVKVAESWVHDNVTRYFNGGNRVRIEYVAVGEEPFLQS 127

Query: 134 YNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDGQFRSDILDEM 193
           Y   Y      A  NIQ AL +A  + ++KV  P + D + S + +PS G FR+D+   M
Sbjct: 128 YGNQYKPFVIGAAMNIQNALVKANLANEVKVVVPSSFDSFLSESGRPSSGHFRADLNKTM 187

Query: 194 KDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKKYTNVFDANFD 253
            +++ FL+++ + F V I PFLS + N +  +DF+ F E  K+  D  K Y N FD ++D
Sbjct: 188 IELLSFLTKHHSPFFVTISPFLSFHQNKNISLDFSLFKETAKAHKDGRKTYRNSFDLSYD 247

Query: 254 TLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASKKGTPMRPNEK 313
           TLV +L  IG  ++ I+V ++GWPTDG + AT   A+ F+ GL+  L  +K T   P   
Sbjct: 248 TLVSALFTIGFSEVDIVVSKIGWPTDGAENATSLTAEAFFKGLIVHL--EKKTASLPRPP 307

Query: 314 LEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLVAAKGVQYLEH 373
           +E Y+  LLDED +++  G FERHWG+F +DG+ K++       N K  V A+ VQYL  
Sbjct: 308 VETYIESLLDEDQRNLSAGNFERHWGVFTFDGQAKYNFSF----NHKNQVNAQNVQYLPP 367

Query: 374 KWCVVKNSVKDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAYNMYFQMQDQS 433
           KWCVV N+ KDL   S++   AC+++DCTS+  G SC+ +   GN+SYA+N  +Q  D S
Sbjct: 368 KWCVVNNN-KDLSNASARALEACAVADCTSILPGGSCSGIRWPGNVSYAFNSLYQQNDHS 427

Query: 434 VEACVFGESAEIVTRNASVGSCLFPIQIDS 461
            E+C FG    I T + S  +C F IQ+D+
Sbjct: 428 AESCNFGGLGLITTVDPSEDNCRFSIQLDT 450

BLAST of CsaV3_2G016670 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 7.8e-59
Identity = 150/456 (32.89%), Postives = 240/456 (52.63%), Query Frame = 0

Query: 9   AVTWTVLIVVTTTITSVVQAHLGANWGTMASHPLNPNIVVNLLEDNGIKKVKLFDSDSWT 68
           ++ + V +++    ++  Q+ LG N+G ++ +  +    VNLL+   I+KV+LF ++   
Sbjct: 9   SLLFLVFLLLQNFYSANSQSFLGVNYGQLSDNLPSLQATVNLLKSTTIQKVRLFGAEPAV 68

Query: 69  VSALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYIAVGNEPFL 128
           + A A + +E ++G  N  + +LAS+ N A  +VK NV +  +    NI  I VGNE  L
Sbjct: 69  IKAFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMS--FYPASNIIAITVGNE-VL 128

Query: 129 TAYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDGQFRSDILD 188
           T+ +   I    PAMQN+Q AL+ A    K+KV+  ++A    S +  PS G F   + D
Sbjct: 129 TSGDQKLISQLLPAMQNVQNALNAASLGGKVKVS-TVHAMAVLSQSYPPSSGVFNPGLGD 188

Query: 189 EMKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAF--FDEN-GKSINDKGKKYTNVF 248
            MK +++F S NDA FM++ YP+ + Y N   P   AF  F  N G+  +  G KYTN+F
Sbjct: 189 TMKALLQFQSANDAPFMISPYPYFA-YKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMF 248

Query: 249 DANFDTLVWSLKKIGLGDLKIIVGEVGWPTDGNK---FATVELAKRFYDGLLKKLASKKG 308
           DA  D +  +L  +G  D++I+V E GWP  G+      +++ AK +   L+  L SK G
Sbjct: 249 DAQVDAVHSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVG 308

Query: 309 TPMRPNEKLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLD-LTGKGNDKRLVA 368
           TP+ P + ++ YLF L DED K+      E+++GLF+ DG   + +  L    N      
Sbjct: 309 TPLMPGKSIDTYLFSLYDEDKKT--GASSEKYFGLFKPDGSTTYDVGLLKNTQNPTTPAT 368

Query: 369 AKGVQYLEHKWCVVKNSVKDLGTISSQIDYACSMS-DCTSLGYGSSCNNLNS-RGNISYA 428
                     WCV K  V D   ++  I+YAC    DC  +  G +C   N+ + + +Y 
Sbjct: 369 PTPTPKAAGSWCVPKPGVSD-DQLTGNINYACGQGIDCGPIQPGGACFEPNTVKAHAAYV 428

Query: 429 YNMYFQMQDQSVEACVFGESAEIVTRNASVGSCLFP 456
            N+Y+Q   ++   C F ++A +   N S G+C FP
Sbjct: 429 MNLYYQSAGRNSWNCDFSQTATLTNTNPSYGACNFP 456

BLAST of CsaV3_2G016670 vs. ExPASy TrEMBL
Match: A0A0A0LLR5 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G277010 PE=3 SV=1)

HSP 1 Score: 979.5 bits (2531), Expect = 4.9e-282
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0

Query: 1   MTGATFAIAVTWTVLIVVTTTITSVVQAHLGANWGTMASHPLNPNIVVNLLEDNGIKKVK 60
           MTGATFAIAVTWTVLIVVTTTITSVVQAHLGANWGTMASHPLNPNIVVNLLEDNGIKKVK
Sbjct: 1   MTGATFAIAVTWTVLIVVTTTITSVVQAHLGANWGTMASHPLNPNIVVNLLEDNGIKKVK 60

Query: 61  LFDSDSWTVSALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYI 120
           LFDSDSWTVSALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYI
Sbjct: 61  LFDSDSWTVSALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYI 120

Query: 121 AVGNEPFLTAYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDG 180
           AVGNEPFLTAYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDG
Sbjct: 121 AVGNEPFLTAYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDG 180

Query: 181 QFRSDILDEMKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKK 240
           QFRSDILDEMKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKK
Sbjct: 181 QFRSDILDEMKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKK 240

Query: 241 YTNVFDANFDTLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASK 300
           YTNVFDANFDTLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASK
Sbjct: 241 YTNVFDANFDTLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASK 300

Query: 301 KGTPMRPNEKLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLV 360
           KGTPMRPNEKLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLV
Sbjct: 301 KGTPMRPNEKLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLV 360

Query: 361 AAKGVQYLEHKWCVVKNSVKDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAY 420
           AAKGVQYLEHKWCVVKNSVKDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAY
Sbjct: 361 AAKGVQYLEHKWCVVKNSVKDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAY 420

Query: 421 NMYFQMQDQSVEACVFGESAEIVTRNASVGSCLFPIQIDSIGQRLTPMNIVVFTATVLIG 480
           NMYFQMQDQSVEACVFGESAEIVTRNASVGSCLFPIQIDSIGQRLTPMNIVVFTATVLIG
Sbjct: 421 NMYFQMQDQSVEACVFGESAEIVTRNASVGSCLFPIQIDSIGQRLTPMNIVVFTATVLIG 480

Query: 481 LVLSMYV 488
           LVLSMYV
Sbjct: 481 LVLSMYV 487

BLAST of CsaV3_2G016670 vs. ExPASy TrEMBL
Match: A0A5A7TQX9 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G00550 PE=3 SV=1)

HSP 1 Score: 891.0 bits (2301), Expect = 2.3e-255
Identity = 432/487 (88.71%), Postives = 463/487 (95.07%), Query Frame = 0

Query: 1   MTGATFAIAVTWTVLIVVTTTITSVVQAHLGANWGTMASHPLNPNIVVNLLEDNGIKKVK 60
           M  ATFAIAVTWTVLIVVTTT+TSVVQA+LG NWG+MASHPLNPNIVVNLL+DNGIKKVK
Sbjct: 1   MASATFAIAVTWTVLIVVTTTVTSVVQANLGVNWGSMASHPLNPNIVVNLLKDNGIKKVK 60

Query: 61  LFDSDSWTVSALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYI 120
           LFDSDSWTV+AL+GSKIETIVGIPNDQLESLA++YNHAKDWVKEN+TAHIYDGGVNIRY+
Sbjct: 61  LFDSDSWTVNALSGSKIETIVGIPNDQLESLATNYNHAKDWVKENITAHIYDGGVNIRYV 120

Query: 121 AVGNEPFLTAYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDG 180
           AVGNEPFLTAYNGTY+KLTFPAMQNIQKAL+EAGY+KKIKVTCPLNADVYESA+N PSDG
Sbjct: 121 AVGNEPFLTAYNGTYVKLTFPAMQNIQKALNEAGYNKKIKVTCPLNADVYESASNHPSDG 180

Query: 181 QFRSDILDEMKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKK 240
           QFRSDILDEMK+IV+FL RN+AAFMVNIYPFLSLYLN DFPVDFAF DENG+SINDKGK 
Sbjct: 181 QFRSDILDEMKEIVQFLDRNNAAFMVNIYPFLSLYLNPDFPVDFAFMDENGRSINDKGKT 240

Query: 241 YTNVFDANFDTLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASK 300
           YTNV DAN+DTLVWSLKKIG GD+KIIVGEVGWPTDGNKFATV LAKRFYDGLLKKLASK
Sbjct: 241 YTNVLDANYDTLVWSLKKIGHGDMKIIVGEVGWPTDGNKFATVGLAKRFYDGLLKKLASK 300

Query: 301 KGTPMRPNEKLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLV 360
           KGTPMRPNEKLEVYLFGL DED KSIQPGFFERHWGLFRYDGKPKF +D TGK NDK LV
Sbjct: 301 KGTPMRPNEKLEVYLFGLFDEDTKSIQPGFFERHWGLFRYDGKPKFPIDFTGKDNDKMLV 360

Query: 361 AAKGVQYLEHKWCVVKNSVKDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAY 420
           AAKGVQYLEHKWCVVKNSVKDL TISSQ+DYACSMSDCT+LGYGSSCNNLN++GNISYAY
Sbjct: 361 AAKGVQYLEHKWCVVKNSVKDLRTISSQVDYACSMSDCTTLGYGSSCNNLNAKGNISYAY 420

Query: 421 NMYFQMQDQSVEACVFGESAEIVTRNASVGSCLFPIQIDSIGQRLTPMNIVVFTATVLIG 480
           NMYFQMQDQSVEACVFG+SAEIVT+NASVG+CLFPIQI S+GQRL  MNIVVF  TVL+ 
Sbjct: 421 NMYFQMQDQSVEACVFGDSAEIVTKNASVGNCLFPIQIVSVGQRLMTMNIVVFVTTVLVS 480

Query: 481 LVLSMYV 488
           L+LSMYV
Sbjct: 481 LMLSMYV 487

BLAST of CsaV3_2G016670 vs. ExPASy TrEMBL
Match: A0A1S3C7C2 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497517 PE=3 SV=1)

HSP 1 Score: 891.0 bits (2301), Expect = 2.3e-255
Identity = 432/487 (88.71%), Postives = 463/487 (95.07%), Query Frame = 0

Query: 1   MTGATFAIAVTWTVLIVVTTTITSVVQAHLGANWGTMASHPLNPNIVVNLLEDNGIKKVK 60
           M  ATFAIAVTWTVLIVVTTT+TSVVQA+LG NWG+MASHPLNPNIVVNLL+DNGIKKVK
Sbjct: 1   MASATFAIAVTWTVLIVVTTTVTSVVQANLGVNWGSMASHPLNPNIVVNLLKDNGIKKVK 60

Query: 61  LFDSDSWTVSALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYI 120
           LFDSDSWTV+AL+GSKIETIVGIPNDQLESLA++YNHAKDWVKEN+TAHIYDGGVNIRY+
Sbjct: 61  LFDSDSWTVNALSGSKIETIVGIPNDQLESLATNYNHAKDWVKENITAHIYDGGVNIRYV 120

Query: 121 AVGNEPFLTAYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDG 180
           AVGNEPFLTAYNGTY+KLTFPAMQNIQKAL+EAGY+KKIKVTCPLNADVYESA+N PSDG
Sbjct: 121 AVGNEPFLTAYNGTYVKLTFPAMQNIQKALNEAGYNKKIKVTCPLNADVYESASNHPSDG 180

Query: 181 QFRSDILDEMKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKK 240
           QFRSDILDEMK+IV+FL RN+AAFMVNIYPFLSLYLN DFPVDFAF DENG+SINDKGK 
Sbjct: 181 QFRSDILDEMKEIVQFLDRNNAAFMVNIYPFLSLYLNPDFPVDFAFMDENGRSINDKGKT 240

Query: 241 YTNVFDANFDTLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASK 300
           YTNV DAN+DTLVWSLKKIG GD+KIIVGEVGWPTDGNKFATV LAKRFYDGLLKKLASK
Sbjct: 241 YTNVLDANYDTLVWSLKKIGHGDMKIIVGEVGWPTDGNKFATVGLAKRFYDGLLKKLASK 300

Query: 301 KGTPMRPNEKLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLV 360
           KGTPMRPNEKLEVYLFGL DED KSIQPGFFERHWGLFRYDGKPKF +D TGK NDK LV
Sbjct: 301 KGTPMRPNEKLEVYLFGLFDEDTKSIQPGFFERHWGLFRYDGKPKFPIDFTGKDNDKMLV 360

Query: 361 AAKGVQYLEHKWCVVKNSVKDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAY 420
           AAKGVQYLEHKWCVVKNSVKDL TISSQ+DYACSMSDCT+LGYGSSCNNLN++GNISYAY
Sbjct: 361 AAKGVQYLEHKWCVVKNSVKDLRTISSQVDYACSMSDCTTLGYGSSCNNLNAKGNISYAY 420

Query: 421 NMYFQMQDQSVEACVFGESAEIVTRNASVGSCLFPIQIDSIGQRLTPMNIVVFTATVLIG 480
           NMYFQMQDQSVEACVFG+SAEIVT+NASVG+CLFPIQI S+GQRL  MNIVVF  TVL+ 
Sbjct: 421 NMYFQMQDQSVEACVFGDSAEIVTKNASVGNCLFPIQIVSVGQRLMTMNIVVFVTTVLVS 480

Query: 481 LVLSMYV 488
           L+LSMYV
Sbjct: 481 LMLSMYV 487

BLAST of CsaV3_2G016670 vs. ExPASy TrEMBL
Match: A0A6J1EQB3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111434805 PE=3 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 6.3e-213
Identity = 363/483 (75.16%), Postives = 420/483 (86.96%), Query Frame = 0

Query: 5   TFAIAVTWTVLIVVTTTITSVVQAHL--GANWGTMASHPLNPNIVVNLLEDNGIKKVKLF 64
           +FAIAVTW  L+ +TT    VV+A+L  G NWGT+ASHP+NP+IVVNLL+DNGIKKVKLF
Sbjct: 6   SFAIAVTWATLMALTT----VVEANLDVGVNWGTIASHPINPSIVVNLLKDNGIKKVKLF 65

Query: 65  DSDSWTVSALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYIAV 124
           DSDSW + ALAGSKIET+V IPNDQLE  A+DY+ AKDWVKENVT +IYDGGV+IRY+AV
Sbjct: 66  DSDSWIMGALAGSKIETVVAIPNDQLEKFANDYDLAKDWVKENVTTYIYDGGVDIRYVAV 125

Query: 125 GNEPFLTAYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDGQF 184
           GNEPFLT +NG+YIKLTFPAMQN+QKAL++AG+  KIKVT PLNADVYES +NQPSDG+F
Sbjct: 126 GNEPFLTGFNGSYIKLTFPAMQNVQKALNDAGHGDKIKVTTPLNADVYESPSNQPSDGRF 185

Query: 185 RSDILDEMKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKKYT 244
           RSDI D MK+IV FLS N+A FMVNIYPFLSLY N DFP+DFAF D +GKSINDKGK YT
Sbjct: 186 RSDIHDTMKNIVHFLSSNNAPFMVNIYPFLSLYQNPDFPIDFAFLDSSGKSINDKGKTYT 245

Query: 245 NVFDANFDTLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASKKG 304
           NVFDAN+DTLVWSLKKIG GDLKI+VGEVGWPTD NK+A +ELAKRFYDGL K LASKKG
Sbjct: 246 NVFDANYDTLVWSLKKIGEGDLKIVVGEVGWPTDANKYANLELAKRFYDGLFKNLASKKG 305

Query: 305 TPMRPNEKLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLVAA 364
           TPMRP+EKLE+YLF L DED+KS+ PGFFERHWG+FRYDGKPKF +DL G GNDK  VAA
Sbjct: 306 TPMRPDEKLEIYLFSLFDEDMKSVLPGFFERHWGIFRYDGKPKFPMDLAGNGNDKMPVAA 365

Query: 365 KGVQYLEHKWCVVKNSVKDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAYNM 424
           KGVQYLE KWCV+KNS++DL +  SQIDYACS+SDCT+L +GSSCN+LN+RGN S+AYNM
Sbjct: 366 KGVQYLEKKWCVIKNSIEDLTSEVSQIDYACSLSDCTTLVFGSSCNDLNARGNFSFAYNM 425

Query: 425 YFQMQDQSVEACVFGESAEIVTRNASVGSCLFPIQIDSIGQRLTPMNIVVFTATVLIGLV 484
           YFQMQDQSVEAC FG SAEIVT+NASVG+CLFPIQI S GQ+ T MN+V F  T+ + ++
Sbjct: 426 YFQMQDQSVEACDFGGSAEIVTKNASVGTCLFPIQIVSAGQK-TMMNVVPFVFTIFVTIL 483

Query: 485 LSM 486
            S+
Sbjct: 486 FSV 483

BLAST of CsaV3_2G016670 vs. ExPASy TrEMBL
Match: A0A6J1IB77 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111473519 PE=3 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 5.4e-212
Identity = 360/480 (75.00%), Postives = 417/480 (86.88%), Query Frame = 0

Query: 5   TFAIAVTWTVLIVVTTTITSVVQAHL--GANWGTMASHPLNPNIVVNLLEDNGIKKVKLF 64
           +FAIAVTW  L+ +TT    VV+A+L  G NWGT+ASHP+NP+IVVNLL+DNGI KVKLF
Sbjct: 6   SFAIAVTWATLMALTT----VVEANLDVGVNWGTIASHPINPSIVVNLLKDNGINKVKLF 65

Query: 65  DSDSWTVSALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYIAV 124
           DSDSW + +LAGSKIET+V IPNDQLE  A+DY+ AKDWVKENVT +IY GGV+IRY+AV
Sbjct: 66  DSDSWIMGSLAGSKIETVVAIPNDQLEKFANDYDLAKDWVKENVTTYIYHGGVDIRYVAV 125

Query: 125 GNEPFLTAYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDGQF 184
           GNEPFLT +NG+YIKLTFPAMQNIQKAL++AG+  KIKVT PLNADVYES +NQPSDG+F
Sbjct: 126 GNEPFLTDFNGSYIKLTFPAMQNIQKALNDAGHGDKIKVTTPLNADVYESPSNQPSDGRF 185

Query: 185 RSDILDEMKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKKYT 244
           RSDI D MK+IV FLS N+A FMVNIYPFLSLY N  FP+DFAF D +GKSINDKGK YT
Sbjct: 186 RSDIHDTMKNIVHFLSSNNAPFMVNIYPFLSLYQNPHFPMDFAFLDSSGKSINDKGKTYT 245

Query: 245 NVFDANFDTLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASKKG 304
           NVFDAN+DTLVWSLKKIG GDLKI+VGEVGWPTD NK+A +ELAKRFYDGL K LASKKG
Sbjct: 246 NVFDANYDTLVWSLKKIGEGDLKIVVGEVGWPTDANKYANLELAKRFYDGLFKNLASKKG 305

Query: 305 TPMRPNEKLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLVAA 364
           TPMRP+EKLE+YLF L DED+KS+ PGFFERHWG+FRYDGKPKF +DL G GNDK  VAA
Sbjct: 306 TPMRPDEKLEIYLFSLFDEDMKSVLPGFFERHWGIFRYDGKPKFPMDLAGNGNDKMPVAA 365

Query: 365 KGVQYLEHKWCVVKNSVKDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAYNM 424
           KGVQYLE KWCV+KNS++DL +  SQIDYACS+SDCT+L +GSSCN+LN+RGN S+AYNM
Sbjct: 366 KGVQYLEKKWCVIKNSIEDLTSEVSQIDYACSLSDCTTLAFGSSCNDLNARGNFSFAYNM 425

Query: 425 YFQMQDQSVEACVFGESAEIVTRNASVGSCLFPIQIDSIGQRLTPMNIVVFTATVLIGLV 483
           YFQMQDQSVEAC FG SAEIVT+NASVG+CLFPIQI S GQ+LT MN++ F +TV + ++
Sbjct: 426 YFQMQDQSVEACDFGGSAEIVTKNASVGTCLFPIQIVSAGQKLTMMNVIPFVSTVFVTIL 481

BLAST of CsaV3_2G016670 vs. TAIR 10
Match: AT5G64790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 576.6 bits (1485), Expect = 1.8e-164
Identity = 288/477 (60.38%), Postives = 360/477 (75.47%), Query Frame = 0

Query: 7   AIAVTWTVLIVVTTTITSVVQAHLGANWGTMASHPLNPNIVVNLLEDNGIKKVKLFDSDS 66
           A+ V    +I+   T+ S+V    G NWG +ASHPLNPNIVV +L+DN I KVKLFD+DS
Sbjct: 6   AMLVNVGTVIMTVLTLASLV-GGFGVNWGNIASHPLNPNIVVQMLKDNKINKVKLFDADS 65

Query: 67  WTVSALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYIAVGNEP 126
           WT++ALAG+ +E +VGIPN+ LESLA DY++AKDWVKENVT +I  GGV+I+Y+AVGNEP
Sbjct: 66  WTMNALAGTGMEVMVGIPNNLLESLADDYDNAKDWVKENVTQYIRKGGVDIKYVAVGNEP 125

Query: 127 FLTAYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDGQFRSDI 186
           FL+AYNG+++K TFPA++NI KAL EAG++  +K T P NA+VY+SA ++PS+G FR D+
Sbjct: 126 FLSAYNGSFLKTTFPALKNIHKALKEAGHTNIMKATIPQNAEVYQSANDKPSEGDFRKDV 185

Query: 187 LDEMKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKKYTNVFD 246
              M DIV F   ND  F VNIYPFLSLYLN  FPV+FAF D +G+++ DKGK Y NVFD
Sbjct: 186 KQTMLDIVNFFHENDLPFTVNIYPFLSLYLNEHFPVEFAFLDGDGQTMTDKGKNYDNVFD 245

Query: 247 ANFDTLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASKKGTPMR 306
           AN+DTLV++LKK G+ D+KIIVGEVGWPTDG+K+A+ +LA++FY GL+K+LA   GTP R
Sbjct: 246 ANYDTLVYALKKAGIHDMKIIVGEVGWPTDGHKYASPKLAEKFYAGLMKRLAKDGGTPTR 305

Query: 307 PNEKLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLVAAKGVQ 366
           P E+LEVYLFG LDED+KSI PG FERHWG+FRYDG PKF LD TG+G     VAAKGVQ
Sbjct: 306 P-ERLEVYLFGFLDEDMKSILPGPFERHWGIFRYDGTPKFMLDFTGQGRQMVPVAAKGVQ 365

Query: 367 YLEHKWCVVKNSVKDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAYNMYFQM 426
           YLE +WCVV     +L  +   +DYAC   DCT++  GS+C+ L    NISYA+NMYFQ+
Sbjct: 366 YLEKQWCVVNKDTVNLDEVGPDLDYACYHGDCTAMEAGSTCSKLTKVQNISYAFNMYFQI 425

Query: 427 QDQSVEACVFGESAEIVTRNASVGSCLFPIQIDSIGQRLTPMNIVVFTATVLIGLVL 484
           QDQ V AC F  +A I   NASVGSCLFP+QI S G     +N  VF   V+ GLVL
Sbjct: 426 QDQDVRACDFKGAAMITKVNASVGSCLFPVQIVS-GSDDFRINF-VFGRFVVFGLVL 478

BLAST of CsaV3_2G016670 vs. TAIR 10
Match: AT1G64760.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 533.9 bits (1374), Expect = 1.4e-151
Identity = 257/431 (59.63%), Postives = 323/431 (74.94%), Query Frame = 0

Query: 30  LGANWGTMASHPLNPNIVVNLLEDNGIKKVKLFDSDSWTVSALAGSKIETIVGIPNDQLE 89
           LG NWGTMA+H L P  VV +L+DN I KVKLFD+D  T+ ALAGS +E +V IPNDQL+
Sbjct: 25  LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVMVAIPNDQLK 84

Query: 90  SLASDYNHAKDWVKENVTAHIYDGGVNIRYIAVGNEPFLTAYNGTYIKLTFPAMQNIQKA 149
            + S Y+ AKDWV++NVT + +DGGVNI ++AVGNEPFL +YNG++I LTFPA+ NIQ A
Sbjct: 85  VMTS-YDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFPALANIQNA 144

Query: 150 LDEAGYSKKIKVTCPLNADVYESATNQ--PSDGQFRSDILDEMKDIVRFLSRNDAAFMVN 209
           L+EAG    +K T PLNADVY+S  +   PS G+FR DI+ +M  IV FL +N+A   +N
Sbjct: 145 LNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGKNNAPITIN 204

Query: 210 IYPFLSLYLNSDFPVDFAFFDENGKSINDKGKKYTNVFDANFDTLVWSLKKIGLGDLKII 269
           IYPFLSLY N DFP+++AFFD   + IND G  YTNVFDANFDTLV SLK +G GD+ II
Sbjct: 205 IYPFLSLYGNDDFPLNYAFFD-GAEPINDNGIDYTNVFDANFDTLVSSLKAVGHGDMPII 264

Query: 270 VGEVGWPTDGNKFATVELAKRFYDGLLKKLASKKGTPMRPNEKLEVYLFGLLDEDLKSIQ 329
           VGEVGWPT+G+K A    A RFY+GLL +L + KGTP+RP   +EVYLFGLLDED KSI 
Sbjct: 265 VGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPT-YIEVYLFGLLDEDAKSIA 324

Query: 330 PGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLVAAKGVQYLEHKWCVVKNSVKDLGTISS 389
           PG FERHWG+F++DG+PKF +DL+G+G  K L+ A+ V YL +KWC      KDL  +++
Sbjct: 325 PGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAKDLTKLAA 384

Query: 390 QIDYACSMSDCTSLGYGSSCNNLNSRGNISYAYNMYFQMQDQSVEACVFGESAEIVTRNA 449
            IDYAC+ SDCT+LGYGSSCN L++ GN SYA+NM+FQ+++Q   AC F   A I T+N 
Sbjct: 385 NIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGLATITTQNI 444

Query: 450 SVGSCLFPIQI 459
           S G C FPIQI
Sbjct: 445 SQGQCNFPIQI 452

BLAST of CsaV3_2G016670 vs. TAIR 10
Match: AT1G64760.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 533.9 bits (1374), Expect = 1.4e-151
Identity = 257/431 (59.63%), Postives = 323/431 (74.94%), Query Frame = 0

Query: 30  LGANWGTMASHPLNPNIVVNLLEDNGIKKVKLFDSDSWTVSALAGSKIETIVGIPNDQLE 89
           LG NWGTMA+H L P  VV +L+DN I KVKLFD+D  T+ ALAGS +E +V IPNDQL+
Sbjct: 25  LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVMVAIPNDQLK 84

Query: 90  SLASDYNHAKDWVKENVTAHIYDGGVNIRYIAVGNEPFLTAYNGTYIKLTFPAMQNIQKA 149
            + S Y+ AKDWV++NVT + +DGGVNI ++AVGNEPFL +YNG++I LTFPA+ NIQ A
Sbjct: 85  VMTS-YDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFPALANIQNA 144

Query: 150 LDEAGYSKKIKVTCPLNADVYESATNQ--PSDGQFRSDILDEMKDIVRFLSRNDAAFMVN 209
           L+EAG    +K T PLNADVY+S  +   PS G+FR DI+ +M  IV FL +N+A   +N
Sbjct: 145 LNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGKNNAPITIN 204

Query: 210 IYPFLSLYLNSDFPVDFAFFDENGKSINDKGKKYTNVFDANFDTLVWSLKKIGLGDLKII 269
           IYPFLSLY N DFP+++AFFD   + IND G  YTNVFDANFDTLV SLK +G GD+ II
Sbjct: 205 IYPFLSLYGNDDFPLNYAFFD-GAEPINDNGIDYTNVFDANFDTLVSSLKAVGHGDMPII 264

Query: 270 VGEVGWPTDGNKFATVELAKRFYDGLLKKLASKKGTPMRPNEKLEVYLFGLLDEDLKSIQ 329
           VGEVGWPT+G+K A    A RFY+GLL +L + KGTP+RP   +EVYLFGLLDED KSI 
Sbjct: 265 VGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPT-YIEVYLFGLLDEDAKSIA 324

Query: 330 PGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLVAAKGVQYLEHKWCVVKNSVKDLGTISS 389
           PG FERHWG+F++DG+PKF +DL+G+G  K L+ A+ V YL +KWC      KDL  +++
Sbjct: 325 PGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAKDLTKLAA 384

Query: 390 QIDYACSMSDCTSLGYGSSCNNLNSRGNISYAYNMYFQMQDQSVEACVFGESAEIVTRNA 449
            IDYAC+ SDCT+LGYGSSCN L++ GN SYA+NM+FQ+++Q   AC F   A I T+N 
Sbjct: 385 NIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGLATITTQNI 444

Query: 450 SVGSCLFPIQI 459
           S G C FPIQI
Sbjct: 445 SQGQCNFPIQI 452

BLAST of CsaV3_2G016670 vs. TAIR 10
Match: AT2G19440.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 531.2 bits (1367), Expect = 8.9e-151
Identity = 254/431 (58.93%), Postives = 324/431 (75.17%), Query Frame = 0

Query: 30  LGANWGTMASHPLNPNIVVNLLEDNGIKKVKLFDSDSWTVSALAGSKIETIVGIPNDQLE 89
           LG NWGTMA+H L P  VV +L+DN I KVKLFD+D  T+SAL+GS +E +V IPNDQL+
Sbjct: 21  LGVNWGTMATHKLPPKKVVQMLKDNNINKVKLFDADETTMSALSGSGLEVMVAIPNDQLK 80

Query: 90  SLASDYNHAKDWVKENVTAHIYDGGVNIRYIAVGNEPFLTAYNGTYIKLTFPAMQNIQKA 149
            + S Y+ AKDWV +NVT + ++GGVNI ++AVGNEPFL +YNG++I LTFPA+QNIQ A
Sbjct: 81  VMGS-YDRAKDWVHKNVTRYNFNGGVNITFVAVGNEPFLKSYNGSFINLTFPALQNIQNA 140

Query: 150 LDEAGYSKKIKVTCPLNADVYESATNQ--PSDGQFRSDILDEMKDIVRFLSRNDAAFMVN 209
           L+EAG    +K T PLNADVY+S ++   PS G+FR DI+ +M  IV FL  N A   +N
Sbjct: 141 LNEAGLGSSVKATVPLNADVYDSPSSNPVPSAGRFRPDIIGQMTQIVDFLGNNSAPITIN 200

Query: 210 IYPFLSLYLNSDFPVDFAFFDENGKSINDKGKKYTNVFDANFDTLVWSLKKIGLGDLKII 269
           IYPFLSLY N DFP+++AFFD   K ++D G  YTNVFDANFDTLV +LK +G GD+ II
Sbjct: 201 IYPFLSLYGNDDFPLNYAFFD-GAKPVDDNGIAYTNVFDANFDTLVSALKAVGHGDMPII 260

Query: 270 VGEVGWPTDGNKFATVELAKRFYDGLLKKLASKKGTPMRPNEKLEVYLFGLLDEDLKSIQ 329
           VGEVGWPT+G+K A    A RFY+GLL +L   +GTP+RP   +EVYLFGLLDED KSI 
Sbjct: 261 VGEVGWPTEGDKHANSGSAYRFYNGLLPRLGENRGTPLRPT-YIEVYLFGLLDEDAKSIA 320

Query: 330 PGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLVAAKGVQYLEHKWCVVKNSVKDLGTISS 389
           PG FERHWG+F++DG+PKF +DL+G+G +K L+ A+ V Y   KWC+     KDL  +++
Sbjct: 321 PGEFERHWGIFKFDGQPKFPIDLSGQGQNKLLIGAENVTYQPKKWCMFNTEAKDLTKLAA 380

Query: 390 QIDYACSMSDCTSLGYGSSCNNLNSRGNISYAYNMYFQMQDQSVEACVFGESAEIVTRNA 449
            IDYAC+ SDCT+LGYGSSCN L++ GN SYA+NMYFQ+++Q  +AC+F   A I T+N 
Sbjct: 381 NIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMYFQVKNQDEDACIFQGLATITTKNI 440

Query: 450 SVGSCLFPIQI 459
           S G C FPIQI
Sbjct: 441 SQGQCNFPIQI 448

BLAST of CsaV3_2G016670 vs. TAIR 10
Match: AT5G18220.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 514.6 bits (1324), Expect = 8.6e-146
Identity = 265/456 (58.11%), Postives = 330/456 (72.37%), Query Frame = 0

Query: 13  TVLIVVTTTITSVVQ---AHLGANWGTMASHPLNPNIVVNLLEDNGIKKVKLFDSDSWTV 72
           T  I +  TI S++    + LG NWGTM++H L P  VV +L+DN +KKVKLFD+D+ T+
Sbjct: 10  TAGIFLCITIVSLLSGDVSALGVNWGTMSTHQLPPKTVVQMLKDNNVKKVKLFDADTNTM 69

Query: 73  SALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYIAVGNEPFLT 132
            ALAGS IE +V IPNDQL+++ S YN AKDWV+ N+T   ++  V I+Y+AVGNEPFLT
Sbjct: 70  VALAGSGIEVMVAIPNDQLKAMGS-YNRAKDWVRRNITR--FNDDVKIKYVAVGNEPFLT 129

Query: 133 AYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQ--PSDGQFRSDIL 192
           AYNG++I LT+PA+ NIQKAL+EAG    IK T PLNADVY S      PS G FR DI 
Sbjct: 130 AYNGSFINLTYPALFNIQKALNEAGVGDFIKATVPLNADVYNSPLENPVPSAGSFRQDIF 189

Query: 193 DEMKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGK-SINDKGKKYTNVFD 252
           +EMK IV FL+ N A F VNIYPFLSLYL+SDFP D+AFF  NG+ +++D G  YTNVFD
Sbjct: 190 EEMKLIVNFLAHNKAPFTVNIYPFLSLYLSSDFPFDYAFF--NGQNTVSDNGVIYTNVFD 249

Query: 253 ANFDTLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASKKGTPMR 312
           ANFDTL+ SLK +G GD+ +IVGEVGWPTDG+K A +  A+RFY GLL KLA+ +GTPMR
Sbjct: 250 ANFDTLLASLKALGHGDMTVIVGEVGWPTDGDKNANIPNAERFYTGLLPKLAANRGTPMR 309

Query: 313 PNEKLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLVAAKGVQ 372
           P   +EVYLFG +DED KSI PG FERHWG+F+YDG+PKF  DL+G G  K L  A+ VQ
Sbjct: 310 PG-YIEVYLFGFIDEDAKSIAPGNFERHWGIFKYDGQPKFPADLSGAGQKKILTGAQNVQ 369

Query: 373 YLEHKWCVVKNSV----KDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAYNM 432
           YL ++WC+   +      +   +   ++YAC+ SDCT+LGYGSSC NL+  GN SYA+NM
Sbjct: 370 YLRNQWCMFNPNALTFSNNTNQLGDNVNYACTFSDCTALGYGSSCGNLDEVGNASYAFNM 429

Query: 433 YFQMQDQSVEACVFGESAEIVTRNASVGSCLFPIQI 459
           YFQ+Q+Q  EAC F   A I TRN S   C FPIQI
Sbjct: 430 YFQVQNQKAEACDFEGLAIITTRNISREQCNFPIQI 459

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004149714.11.0e-281100.00glucan endo-1,3-beta-glucosidase 8 [Cucumis sativus] >KGN61959.1 hypothetical pr... [more]
XP_008457953.14.7e-25588.71PREDICTED: glucan endo-1,3-beta-glucosidase 8-like [Cucumis melo] >KAA0045792.1 ... [more]
XP_038902854.17.6e-23783.57glucan endo-1,3-beta-glucosidase 8-like [Benincasa hispida][more]
KAG7011052.14.5e-21375.36Glucan endo-1,3-beta-glucosidase 8, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022927955.11.3e-21275.16glucan endo-1,3-beta-glucosidase 8-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q6NKW91.9e-15059.63Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 ... [more]
Q93Z088.1e-14153.57Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 ... [more]
Q9M0883.0e-13551.88Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 ... [more]
Q9FGH41.2e-9642.22Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 ... [more]
Q94G867.8e-5932.89Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LLR54.9e-282100.00(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G277010 PE=3... [more]
A0A5A7TQX92.3e-25588.71(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3C7C22.3e-25588.71(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497517 PE=3 SV... [more]
A0A6J1EQB36.3e-21375.16(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111434805 P... [more]
A0A6J1IB775.4e-21275.00(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111473519 PE=... [more]
Match NameE-valueIdentityDescription
AT5G64790.11.8e-16460.38O-Glycosyl hydrolases family 17 protein [more]
AT1G64760.11.4e-15159.63O-Glycosyl hydrolases family 17 protein [more]
AT1G64760.21.4e-15159.63O-Glycosyl hydrolases family 17 protein [more]
AT2G19440.18.9e-15158.93O-Glycosyl hydrolases family 17 protein [more]
AT5G18220.18.6e-14658.11O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 371..454
e-value: 2.9E-30
score: 116.5
IPR012946X8 domainPFAMPF07983X8coord: 371..440
e-value: 1.1E-14
score: 54.9
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 30..349
e-value: 4.1E-69
score: 233.6
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 264..277
NoneNo IPR availableGENE3D1.20.58.1040coord: 367..459
e-value: 3.0E-6
score: 29.4
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 29..357
e-value: 1.3E-111
score: 374.6
NoneNo IPR availablePANTHERPTHR32227:SF290BETA-1,3-GLUCANASEcoord: 16..467
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 16..467
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 31..349

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_2G016670.1CsaV3_2G016670.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds