CsaV3_2G008170 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_2G008170
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	(R)-mandelonitrile lyase 1-like
Location	chr2: 4429927 .. 4432564 (-)
RNA-Seq Expression	CsaV3_2G008170
Synteny	CsaV3_2G008170

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AACAAGAGTCTAACTTGTTATCCAAAACCTAGATAATAATGTAAAATAAGAGTCAACCTTTGTATCTATAAAAACACCACCACATATAGTTGCAAAGTGGATAAAGAAAAGAAAAAGGAAATAAAAATACTAGCATGAAGAACTCTATGGCTAACCAGATTCTCCTCTTCACTCTCATCTCCATCTTTCAATTAGGACTCATCGCCTCCCACACCATCCCTAATCAAGGTAAAACTCCCCCCTTTCTTTATTTACTTACTAAATTTCCTATGTAAAGTTTAAATTTTCGGTTGATTAGTGATTTAGCATGTATCAGAATAGGAGATGTACTTTTTATTAATTTTCCTACCTACTTAATTAATATCAACTTTCACTTAATGATTCTTTTTTGAGTCTACAAGTGTGAGGGAGATTTAACAGAATTTGGAACGTTTTCTTTTTTCAGATACTAGTTACATGAAGTTTGTATATGAGGCAAGTGAGTTACAAGAAAGTGAAGAATATGATTACATAATAATAGGAGGAGGAACAGCAGGATGTCCATTAGCTGCAACATTATCATCTAAATTTTCAGTTCTCCTTCTTGAAAGAGGCAATGACCCTAACAAATATCCCTCAGTGTTAAATGAACAAGGCCTATTGAATGCTTTTGTTGCAGAAGATGATGGCCAAAATCCCTTCCAACATTTCATCTCTGAGGATGGTGTAGAGAACTTAAGAGGGCGTGTTCTCGGCGGTGGTAGCATGATCAATGCCGGGTTTTACTCGAGGGGTCATCGTGACTTCTTTGAAACTGCCGGTGTGGATTGGGACAGGGAATTGGTGGAGAAGGCTTATGAATGGGTTGAAGAAACTGTGGTATCTAAGCCGAGTTTGAGTCCATGGCAAGCTGCTTTTAGAAGTGCATTATTGGAAGGTGGCGTTGGCCATGATAAGGGGTTTGATTTGAGGCACCTTGTGGGGACTAAAACTGGAGGATCTATCTTTGATAACAAAGGAAACAGACATGGAGCTGTAGAACTTCTCAACAAAGGTGAACCTAAAAACCTTAAAGTTGCAACTCAGGCCACAGTCCAAAGAATCATCTTCACTGGTTAGTTCCTAACAAATCCTTGGTTTAGATTTTATGTTGGTTAATGAATTTTATAAAATAAACTATTTTGATTTCTAAACTTTTGTATGCATATGGTTTTAAATGATAAAACTCTTGAAAACATTTACAAATATAGTGATAATATTTTGAAAAGTAGTAGACACTGATAATATTTTGTTATATATTTGAAACCATATATTTGATTCATTTTGTTATACTTGAAAACAATCCTTTTCATATACATATATATGTTTTCTTGCTGCTGTATATATAATATCCACTCACGCTGTATTAGCATATCACAACAAATTTTGTTCGAAGAGGTTGTAATGATAAAATTAGAAGTTATTTAATTTGGAGAAGTTACACAGTGTGACTAATAATTTTACACCATTGGTGTGTAATAGACAAACCGCAGAACTATCATAAATTTGTATCAAAGGGTTATTACGTGAAACACAAATATGTAAATAATAGTGAAGCGAAACAATAACACTATCTTTTTTCACTATTCTAAATTCATAGGTTTATCAGCAAGTGGGGTTTCATATTCTGATTCAAAAGGAAAGTTGCATACAGCATTCATTCGTAAAAAAGGAGAGATCATACTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTGCTAAGTGGGGTTGGCCCAAAATCTTATCTTTCATCCTTAAGACTACCTGTCGTCCTCGATCAACCACACGTCGGGGAATTTATGTCCGATAATCCCCGTTTCAGTCCGACCATCGTTCTTCCATTCCAACTAGTTTCTTCATCTGCACAAGTAGTTGGAACATTAGACCACAATATCCACTTGCAATCTTTTGCTAGCCCTTTACCATTTTTTGCTCCACCATCGTTTAGCCTTCTTCCTCCTCAATTCACTTCAATTGTCCCTAGCTTAGCCATATTTGTTGGTAAATTCTCTGATGTTCATTCTGAAGGCTCACTTCGTTTGAATTCCTCCATTGATGTGAAGGAGAGTCCCATTGTTAGATTCAATTATTATTCACATCCTGATGATCTTGCTCGATGTGTTAGAGGAGTAAGAAAAGTAGGGGATTTGCTTAAAACTCCAACCATGGAAAAGATTAAGACTCAAGACTTAGAGGGTAATAAAAGATTTCAGTTTTTGGGGCTTTCTTTGCCAGAAAATTTGTTGAATGATACCGCTGTTGAAGAATATTGTCAAAAAACAGTGACTACTTATTGGCATTATCATGGAGGATGTTTGGTGGGAAAAGTAGTTGATGATAACCACAAAGTGATCGGAATTGACAATTTACGTGTAGTTGATGGCTCCACTTTCTCTGTCTCACCGGGAACTAATCCTATGGCCACCCTCATGATGCTCGGCCGGTGAGTTCATTAAATATATATATTGCAGTTAATGTTTTAGTAGGTATTTGTACTTGACAGGGGTTTCTATTTTTTAATTACTGTATTTTTTCAGTAAACTAAAATATTTGAAAAACTATTTTGCAGGTATGTTGGCCTCAAGCTACTGCAACAAAGATCAAGTTAAAGAGTGC

mRNA sequence

ATGAAGAACTCTATGGCTAACCAGATTCTCCTCTTCACTCTCATCTCCATCTTTCAATTAGGACTCATCGCCTCCCACACCATCCCTAATCAAGATACTAGTTACATGAAGTTTGTATATGAGGCAAGTGAGTTACAAGAAAGTGAAGAATATGATTACATAATAATAGGAGGAGGAACAGCAGGATGTCCATTAGCTGCAACATTATCATCTAAATTTTCAGTTCTCCTTCTTGAAAGAGGCAATGACCCTAACAAATATCCCTCAGTGTTAAATGAACAAGGCCTATTGAATGCTTTTGTTGCAGAAGATGATGGCCAAAATCCCTTCCAACATTTCATCTCTGAGGATGGTGTAGAGAACTTAAGAGGGCGTGTTCTCGGCGGTGGTAGCATGATCAATGCCGGGTTTTACTCGAGGGGTCATCGTGACTTCTTTGAAACTGCCGGTGTGGATTGGGACAGGGAATTGGTGGAGAAGGCTTATGAATGGGTTGAAGAAACTGTGGTATCTAAGCCGAGTTTGAGTCCATGGCAAGCTGCTTTTAGAAGTGCATTATTGGAAGGTGGCGTTGGCCATGATAAGGGGTTTGATTTGAGGCACCTTGTGGGGACTAAAACTGGAGGATCTATCTTTGATAACAAAGGAAACAGACATGGAGCTGTAGAACTTCTCAACAAAGGTGAACCTAAAAACCTTAAAGTTGCAACTCAGGCCACAGTCCAAAGAATCATCTTCACTGGTTTATCAGCAAGTGGGGTTTCATATTCTGATTCAAAAGGAAAGTTGCATACAGCATTCATTCGTAAAAAAGGAGAGATCATACTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTGCTAAGTGGGGTTGGCCCAAAATCTTATCTTTCATCCTTAAGACTACCTGTCGTCCTCGATCAACCACACGTCGGGGAATTTATGTCCGATAATCCCCGTTTCAGTCCGACCATCGTTCTTCCATTCCAACTAGTTTCTTCATCTGCACAAGTAGTTGGAACATTAGACCACAATATCCACTTGCAATCTTTTGCTAGCCCTTTACCATTTTTTGCTCCACCATCGTTTAGCCTTCTTCCTCCTCAATTCACTTCAATTGTCCCTAGCTTAGCCATATTTGTTGGTAAATTCTCTGATGTTCATTCTGAAGGCTCACTTCGTTTGAATTCCTCCATTGATGTGAAGGAGAGTCCCATTGTTAGATTCAATTATTATTCACATCCTGATGATCTTGCTCGATGTGTTAGAGGAGTAAGAAAAGTAGGGGATTTGCTTAAAACTCCAACCATGGAAAAGATTAAGACTCAAGACTTAGAGGGTAATAAAAGATTTCAGTTTTTGGGGCTTTCTTTGCCAGAAAATTTGTTGAATGATACCGCTGTTGAAGAATATTGTCAAAAAACAGTGACTACTTATTGGCATTATCATGGAGGATGTTTGGTGGGAAAAGTAGTTGATGATAACCACAAAGTGATCGGAATTGACAATTTACGTGTAGTTGATGGCTCCACTTTCTCTGTCTCACCGGGAACTAATCCTATGGCCACCCTCATGATGCTCGGCCGGTATGTTGGCCTCAAGCTACTGCAACAAAGATCAAGTTAA

Coding sequence (CDS)

ATGAAGAACTCTATGGCTAACCAGATTCTCCTCTTCACTCTCATCTCCATCTTTCAATTAGGACTCATCGCCTCCCACACCATCCCTAATCAAGATACTAGTTACATGAAGTTTGTATATGAGGCAAGTGAGTTACAAGAAAGTGAAGAATATGATTACATAATAATAGGAGGAGGAACAGCAGGATGTCCATTAGCTGCAACATTATCATCTAAATTTTCAGTTCTCCTTCTTGAAAGAGGCAATGACCCTAACAAATATCCCTCAGTGTTAAATGAACAAGGCCTATTGAATGCTTTTGTTGCAGAAGATGATGGCCAAAATCCCTTCCAACATTTCATCTCTGAGGATGGTGTAGAGAACTTAAGAGGGCGTGTTCTCGGCGGTGGTAGCATGATCAATGCCGGGTTTTACTCGAGGGGTCATCGTGACTTCTTTGAAACTGCCGGTGTGGATTGGGACAGGGAATTGGTGGAGAAGGCTTATGAATGGGTTGAAGAAACTGTGGTATCTAAGCCGAGTTTGAGTCCATGGCAAGCTGCTTTTAGAAGTGCATTATTGGAAGGTGGCGTTGGCCATGATAAGGGGTTTGATTTGAGGCACCTTGTGGGGACTAAAACTGGAGGATCTATCTTTGATAACAAAGGAAACAGACATGGAGCTGTAGAACTTCTCAACAAAGGTGAACCTAAAAACCTTAAAGTTGCAACTCAGGCCACAGTCCAAAGAATCATCTTCACTGGTTTATCAGCAAGTGGGGTTTCATATTCTGATTCAAAAGGAAAGTTGCATACAGCATTCATTCGTAAAAAAGGAGAGATCATACTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTGCTAAGTGGGGTTGGCCCAAAATCTTATCTTTCATCCTTAAGACTACCTGTCGTCCTCGATCAACCACACGTCGGGGAATTTATGTCCGATAATCCCCGTTTCAGTCCGACCATCGTTCTTCCATTCCAACTAGTTTCTTCATCTGCACAAGTAGTTGGAACATTAGACCACAATATCCACTTGCAATCTTTTGCTAGCCCTTTACCATTTTTTGCTCCACCATCGTTTAGCCTTCTTCCTCCTCAATTCACTTCAATTGTCCCTAGCTTAGCCATATTTGTTGGTAAATTCTCTGATGTTCATTCTGAAGGCTCACTTCGTTTGAATTCCTCCATTGATGTGAAGGAGAGTCCCATTGTTAGATTCAATTATTATTCACATCCTGATGATCTTGCTCGATGTGTTAGAGGAGTAAGAAAAGTAGGGGATTTGCTTAAAACTCCAACCATGGAAAAGATTAAGACTCAAGACTTAGAGGGTAATAAAAGATTTCAGTTTTTGGGGCTTTCTTTGCCAGAAAATTTGTTGAATGATACCGCTGTTGAAGAATATTGTCAAAAAACAGTGACTACTTATTGGCATTATCATGGAGGATGTTTGGTGGGAAAAGTAGTTGATGATAACCACAAAGTGATCGGAATTGACAATTTACGTGTAGTTGATGGCTCCACTTTCTCTGTCTCACCGGGAACTAATCCTATGGCCACCCTCATGATGCTCGGCCGGTATGTTGGCCTCAAGCTACTGCAACAAAGATCAAGTTAA

Protein sequence

MKNSMANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQAAFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQATVQRIIFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSSLRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFAPPSFSLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTYWHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKLLQQRSS*

Homology

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BLAST of CsaV3_2G008170 vs. NCBI nr
Match: XP_031736478.1 ((R)-mandelonitrile lyase 1-like [Cucumis sativus] >KGN61117.1 hypothetical protein Csa_021176 [Cucumis sativus])

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 542/542 (100.00%), Postives = 542/542 (100.00%), Query Frame = 0


Query: 1   MKNSMANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGT 60
           MKNSMANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGT
Sbjct: 1   MKNSMANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGT 60

Query: 61  AGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVE 120
           AGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVE
Sbjct: 61  AGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVE 120

Query: 121 NLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQA 180
           NLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQA
Sbjct: 121 NLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQA 180

Query: 181 AFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQAT 240
           AFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQAT
Sbjct: 181 AFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQAT 240

Query: 241 VQRIIFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSS 300
           VQRIIFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSS
Sbjct: 241 VQRIIFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSS 300

Query: 301 LRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFA 360
           LRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFA
Sbjct: 301 LRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFA 360

Query: 361 PPSFSLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYYSHPDDLA 420
           PPSFSLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYYSHPDDLA
Sbjct: 361 PPSFSLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYYSHPDDLA 420

Query: 421 RCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTY 480
           RCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTY
Sbjct: 421 RCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTY 480

Query: 481 WHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKLLQQR 540
           WHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKLLQQR
Sbjct: 481 WHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKLLQQR 540

Query: 541 SS 543
           SS
Sbjct: 541 SS 542

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BLAST of CsaV3_2G008170 vs. NCBI nr
Match: XP_008451922.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo] >KAA0067298.1 (R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa] >TYK10909.1 (R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1035.8 bits (2677), Expect = 1.3e-298
Identity = 513/538 (95.35%), Postives = 530/538 (98.51%), Query Frame = 0


Query: 5   MANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGTAGCP 64
           MAN ILLFTLISIFQLG+I+SH IPNQDTSYMKFV+EASELQESEEYDYIIIGGGTAGCP
Sbjct: 1   MANHILLFTLISIFQLGVISSHPIPNQDTSYMKFVHEASELQESEEYDYIIIGGGTAGCP 60

Query: 65  LAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRG 124
           LAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRG
Sbjct: 61  LAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRG 120

Query: 125 RVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQAAFRS 184
           RVLGGGSMINAGFYSRGHR+FFETAGVDWD ELVEKAYEWVEETVVSKPSLSPWQAAFRS
Sbjct: 121 RVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYEWVEETVVSKPSLSPWQAAFRS 180

Query: 185 ALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQATVQRI 244
           ALLEGGVGHD GFDL+HLVGTKTGGSIFDNKGNRHGAVELLNKGEP+N KVATQATVQRI
Sbjct: 181 ALLEGGVGHDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKGEPENFKVATQATVQRI 240

Query: 245 IFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSSLRLP 304
           IFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKS L SL+LP
Sbjct: 241 IFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSQLKSLKLP 300

Query: 305 VVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFAPPSF 364
           VVLDQPHVGEFMSDNPRF+PTIVLPFQ+V+SSAQVVGTLD+NIHLQ+FASPLPFFAPPSF
Sbjct: 301 VVLDQPHVGEFMSDNPRFTPTIVLPFQVVASSAQVVGTLDNNIHLQAFASPLPFFAPPSF 360

Query: 365 SLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVR 424
           SLLPPQ TSIVPSLAIFVGKFSDV+SEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVR
Sbjct: 361 SLLPPQSTSIVPSLAIFVGKFSDVYSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVR 420

Query: 425 GVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTYWHYH 484
           GVRKVGDLL+TPTMEKIKTQDLEGNKRFQFLGLSLPENLLND+AVEEYCQKTVTTYWHYH
Sbjct: 421 GVRKVGDLLRTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDSAVEEYCQKTVTTYWHYH 480

Query: 485 GGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKLLQQRSS 543
           GGCLVGKVVDDNHKVIGI+NLRVVDGSTFSVSPGTNPMATLMMLGRYVGLK+LQQRSS
Sbjct: 481 GGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKVLQQRSS 538

[Close]

BLAST of CsaV3_2G008170 vs. NCBI nr
Match: XP_038889699.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 967.6 bits (2500), Expect = 4.4e-278
Identity = 484/542 (89.30%), Postives = 512/542 (94.46%), Query Frame = 0


Query: 1   MKNSMANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGT 60
           MK SMA  ILL  LISIFQLG+ +SHTIP+QDTSY+KFV+EA+EL E+ EYDYIIIGGGT
Sbjct: 1   MKRSMAT-ILLIILISIFQLGVFSSHTIPDQDTSYLKFVHEANELPETGEYDYIIIGGGT 60

Query: 61  AGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVE 120
           AGCPLAATLSSK+SVLLLERG+DPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVE
Sbjct: 61  AGCPLAATLSSKYSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVE 120

Query: 121 NLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQA 180
           N+RGRVLGGGSMINAGFYSRGHR+FFETAGVDWD ELVEKAY+WVE+TVVS+PSLS WQ+
Sbjct: 121 NIRGRVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEQTVVSRPSLSAWQS 180

Query: 181 AFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQAT 240
           AFR ALLE GVG D GFDL+HLVGTKTGGSIFDNKGNRHGAVELLNK EP+NLKV TQA 
Sbjct: 181 AFRGALLEAGVGPDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVGTQAI 240

Query: 241 VQRIIFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSS 300
           V+RIIFTGLSASGVSYSD KGKLHTAFIR+KGEIILSAGAIGSPQLLLLSGVGPK +LSS
Sbjct: 241 VERIIFTGLSASGVSYSDPKGKLHTAFIREKGEIILSAGAIGSPQLLLLSGVGPKYHLSS 300

Query: 301 LRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFA 360
           L+LPVVL QPHVGEFMSDNPRFSPTIVLPFQLV+SSAQVVGTL +NIHLQSFASPLPFFA
Sbjct: 301 LKLPVVLHQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLANNIHLQSFASPLPFFA 360

Query: 361 PPSFSLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYYSHPDDLA 420
           PPSFSLLPPQ TSI+PSLAIFVGKFSDV SEGSLRLNSSIDVKESPIVRFNYYS+ DDLA
Sbjct: 361 PPSFSLLPPQSTSIIPSLAIFVGKFSDVLSEGSLRLNSSIDVKESPIVRFNYYSNHDDLA 420

Query: 421 RCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTY 480
           RCVRGVRKVGDLLKT TMEKIK QDLEGNK FQFLGL LPENL ND+AVEEYCQKTVTTY
Sbjct: 421 RCVRGVRKVGDLLKTQTMEKIKNQDLEGNKGFQFLGLPLPENLWNDSAVEEYCQKTVTTY 480

Query: 481 WHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKLLQQR 540
           WHYHGGCLVGKVVD NHKVIGI+NLRVVDGSTFSVSPGTNPMATLMMLGRYVGLK+LQQR
Sbjct: 481 WHYHGGCLVGKVVDHNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKVLQQR 540

Query: 541 SS 543
           SS
Sbjct: 541 SS 541

[Close]

BLAST of CsaV3_2G008170 vs. NCBI nr
Match: XP_038889315.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 901.4 bits (2328), Expect = 3.9e-258
Identity = 440/542 (81.18%), Postives = 488/542 (90.04%), Query Frame = 0


Query: 1   MKNSMANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGT 60
           M++S +  ++L  +ISIF LGL++SH IPNQD  YMKFV+ A++L   EEYDYIIIGGGT
Sbjct: 1   MEHSRSTILILSLMISIFHLGLLSSHAIPNQDVGYMKFVHSATDLPAKEEYDYIIIGGGT 60

Query: 61  AGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVE 120
           AGCPL++TLSSKFSVLLLERG+DPNKYPSVLNEQGLLNAF AEDDG+NPFQHFISEDGVE
Sbjct: 61  AGCPLSSTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFAAEDDGKNPFQHFISEDGVE 120

Query: 121 NLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQA 180
           N+RGRVLGGGSM+N GFYSRGH+ FFETAGVDWD ELVEKAY+WVEETVVS+P L+ WQ+
Sbjct: 121 NIRGRVLGGGSMVNVGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVVSQPILNAWQS 180

Query: 181 AFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQAT 240
           AFRSA L+ GV  D GFDLRH VGTK GGSIFDNKGNRHGAVELLNK  P N+KV  +AT
Sbjct: 181 AFRSASLDAGVVPDNGFDLRHRVGTKIGGSIFDNKGNRHGAVELLNKANPTNIKVGIEAT 240

Query: 241 VQRIIFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSS 300
           VQRI+F+GLSA GV YSDSKGKLH AFIRK+GEII+SAGA+GSPQLLLLSGVGPKS+LSS
Sbjct: 241 VQRILFSGLSARGVLYSDSKGKLHRAFIRKEGEIIVSAGAVGSPQLLLLSGVGPKSHLSS 300

Query: 301 LRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFA 360
           L+LPVVL QP+VGEFMSDNPRF   I+LPFQLV SS +VVG L  NI++QSFASP PFFA
Sbjct: 301 LKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSPFFA 360

Query: 361 PPSFSLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYYSHPDDLA 420
           PPSFSLLPPQF SIVPSLA+FVGKFS+VHSEGSLRLNSS +VK+SPIVRFNYYSHPDDLA
Sbjct: 361 PPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420

Query: 421 RCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTY 480
           RCV+GVRK+GDLLKT TMEKIKT DLEGNK FQFLGL LPENL ND++VEEYCQKTVTTY
Sbjct: 421 RCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVEEYCQKTVTTY 480

Query: 481 WHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKLLQQR 540
           WHYHGGCLVGKVVD N+KVIGI+NLRVVDGSTF  SPGTNPMATLMMLGRYVGLK+LQQR
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFCDSPGTNPMATLMMLGRYVGLKVLQQR 540

Query: 541 SS 543
           SS
Sbjct: 541 SS 542

[Close]

BLAST of CsaV3_2G008170 vs. NCBI nr
Match: XP_038889314.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 891.7 bits (2303), Expect = 3.1e-255
Identity = 440/542 (81.18%), Postives = 486/542 (89.67%), Query Frame = 0


Query: 1   MKNSMANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGT 60
           M++S A  ++L  +ISI QLG++  + IP +D  YMKFV++AS+L   EEYDYIIIGGGT
Sbjct: 1   MEHSKATILILSLMISISQLGVLTYNAIPIKDVRYMKFVHKASDLPAKEEYDYIIIGGGT 60

Query: 61  AGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVE 120
           AGCPLAATLSSKFSVL+LERG+DPNKYPSVLNEQGLLN F A DDG+NPFQ FISEDGVE
Sbjct: 61  AGCPLAATLSSKFSVLVLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFISEDGVE 120

Query: 121 NLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQA 180
           N+R RVLGGGSM+NAGFYSRGH+ FFETAGVDWD ELVEKAY+WVEETV S+P L+ WQ+
Sbjct: 121 NIRARVLGGGSMVNAGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVASRPILNAWQS 180

Query: 181 AFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQAT 240
           AFRSALLE GV  D GFDLRHLVGTKTGGSIFD+KGNRHGAVELLNK EP N+KVA +AT
Sbjct: 181 AFRSALLEAGVVPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPTNIKVAIEAT 240

Query: 241 VQRIIFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSS 300
           VQ+I+F+GLSA GV YSDSKGKLH AFIRKKGEII+SAGAIGSPQLLLLSGVGPKS+LSS
Sbjct: 241 VQKILFSGLSARGVLYSDSKGKLHRAFIRKKGEIIVSAGAIGSPQLLLLSGVGPKSHLSS 300

Query: 301 LRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFA 360
           L+LPVVL QP+VGEFMSDNPRF   I+LPFQLV SS +VVG L  NI++QSFASP PFFA
Sbjct: 301 LKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSPFFA 360

Query: 361 PPSFSLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYYSHPDDLA 420
           PPSFSLLPPQF SIVPSLA+FVGKFS+VHSEGSLRLNSS +VK+SPIVRFNYYSHPDDLA
Sbjct: 361 PPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420

Query: 421 RCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTY 480
           RCV+GVRK+GDLLKT TMEKIKT DLEGNK FQFLGL LPENL ND++V EYCQKTVTTY
Sbjct: 421 RCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVGEYCQKTVTTY 480

Query: 481 WHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKLLQQR 540
           WHYHGGCLVGKVVD N+KVIGI NLRVVDGSTFS SPGTNPMATLMMLGRYVGLK+LQQR
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKVLQQR 540

Query: 541 SS 543
           SS
Sbjct: 541 SS 542

[Close]

BLAST of CsaV3_2G008170 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 4.9e-155
Identity = 279/549 (50.82%), Postives = 375/549 (68.31%), Query Frame = 0


Query: 1   MKNSMANQILLFTLISIFQLGLIASH---TIPNQDTSYMKFVYEASELQESEEYDYIIIG 60
           M+ S  + ILL   + +  L     H   T  N D SY++F Y+A++L+    YDY+I+G
Sbjct: 1   MEKSTMSAILLVLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVG 60

Query: 61  GGTAGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISED 120
           GGT+GCPLAATLS K+ VL+LERG+ P  YP+VL   G +     EDDG+ P + F+SED
Sbjct: 61  GGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSED 120

Query: 121 GVENLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSP 180
           G++N+RGRVLGG SMINAG Y+R +   +  +GVDWD +LV K YEWVE+T+V KP+  P
Sbjct: 121 GIDNVRGRVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQP 180

Query: 181 WQAAFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVAT 240
           WQ+   +A LE GV  + GF L H  GT+  GS FDNKG RH A ELLNKG   NL+V  
Sbjct: 181 WQSVTGTAFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGV 240

Query: 241 QATVQRIIFT---GLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGP 300
            A+V++IIF+   GL+A+GV Y DS G  H AF+R KGE+I+SAG IG+PQLLLLSGVGP
Sbjct: 241 HASVEKIIFSNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGP 300

Query: 301 KSYLSSLRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFAS 360
           +SYLSSL +PVVL  P+VG+F+ DNPR    I+ P  +  +   V+G + ++ +  SF+S
Sbjct: 301 ESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLG-ISNDFYQCSFSS 360

Query: 361 PLPFFAPPSFSLLPPQFTSIVPS-LAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYY 420
            LPF  PP FS  P     +  S  A F  K +   S GSL L SS +V+ SP V+FNYY
Sbjct: 361 -LPFTTPP-FSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYY 420

Query: 421 SHPDDLARCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYC 480
           S+P DL+ CV G++K+G+LL T  ++  K +DL G + F  LG+ LP++  +D A E +C
Sbjct: 421 SNPTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFC 480

Query: 481 QKTVTTYWHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVG 540
           +++V +YWHYHGGCLVGKV+D + +V GID LRVVDGSTF  +P ++P    +MLGRYVG
Sbjct: 481 RESVASYWHYHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGRYVG 540

Query: 541 LKLLQQRSS 543
           +K+LQ+RS+
Sbjct: 541 IKILQERSA 546

[Close]

BLAST of CsaV3_2G008170 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 7.4e-151
Identity = 273/549 (49.73%), Postives = 372/549 (67.76%), Query Frame = 0


Query: 1   MKNSMANQILLFTLISIFQLGLIASH---TIPNQDTSYMKFVYEASELQESEEYDYIIIG 60
           M+ S  + ILL   I +  L     H   T  + D SY+ F Y+A++L+    YDY+I+G
Sbjct: 1   MEKSTMSAILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVG 60

Query: 61  GGTAGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISED 120
           GGT+GCPLAATLS K+ VL+LERG+ P  YP+VL   G +     EDDG+ P + F+SED
Sbjct: 61  GGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSED 120

Query: 121 GVENLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSP 180
           G++N+RGRVLGG S+INAG Y+R +   +  +GVDWD +LV + YEWVE+T+V KP+   
Sbjct: 121 GIDNVRGRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQS 180

Query: 181 WQAAFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVAT 240
           WQ+  ++A LE GV  + GF L H  GT+  GS FDNKG RH A ELLNKG   NL+V  
Sbjct: 181 WQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGV 240

Query: 241 QATVQRIIFT---GLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGP 300
            A+V++IIF+   GL+A+GV Y DS G  H AF+R KGE+I+SAG IG+PQLLLLSGVGP
Sbjct: 241 HASVEKIIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGP 300

Query: 301 KSYLSSLRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFAS 360
           +SYLSSL +PVVL  P+VG+F+ DNPR    I+ P  +  +   V+G + ++ +  SF+S
Sbjct: 301 ESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLG-ISNDFYQCSFSS 360

Query: 361 PLPFFAPPSFSLLPPQFTSIVPS-LAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYY 420
            LPF  PP F   P     +  S  A F  K +   S GSL L SS +V+ SP V+FNYY
Sbjct: 361 -LPFTTPP-FGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYY 420

Query: 421 SHPDDLARCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYC 480
           S+  DL+ CV G++K+G+LL T  ++  K +DL G + F  LG+ LP++  +D A E +C
Sbjct: 421 SNLTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFC 480

Query: 481 QKTVTTYWHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVG 540
           +++V +YWHYHGGCLVGKV+D + +V GI+ LRVVDGSTF  +P ++P    +MLGRYVG
Sbjct: 481 RESVASYWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGRYVG 540

Query: 541 LKLLQQRSS 543
           +K+LQ+RS+
Sbjct: 541 IKILQERSA 546

[Close]

BLAST of CsaV3_2G008170 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.6e-148
Identity = 270/553 (48.82%), Postives = 367/553 (66.37%), Query Frame = 0


Query: 1   MKNSMANQILLFTLISIFQLGLIASHTIPN---QDTSYMKFVYEASELQESEEYDYIIIG 60
           M  S  + +LL   I +  L      ++ N    D SY+ FVY+A++ +    YDYII+G
Sbjct: 1   MVKSTMSAVLLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVG 60

Query: 61  GGTAGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISED 120
           GGTAGCPLAATLS+ +SVL+LERG+ P +YP++L   G +     EDDG+ P + F+SED
Sbjct: 61  GGTAGCPLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSED 120

Query: 121 GVENLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSP 180
           G++N+RGRVLGG SMINAG Y R +  FF   G++WD +LV + YEWVE+T+V +P    
Sbjct: 121 GIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQT 180

Query: 181 WQAAFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVAT 240
           WQ    +A LE G+  + GF + HL GT+  GS FDN G RH + ELLNKG+P NL+VA 
Sbjct: 181 WQTVIGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAV 240

Query: 241 QATVQRIIF----TGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVG 300
           QA V++IIF    +G++A GV Y+DS G  H AF+R +GE+ILSAG IGSPQLLLLSGVG
Sbjct: 241 QAAVEKIIFSSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVG 300

Query: 301 PKSYLSSLRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFA 360
           P+SYL+SL + VV   P+VG+++ DNPR    I+ P  + +S+  V+G        Q   
Sbjct: 301 PESYLTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSI 360

Query: 361 SPLPFFAPPSFSLLPPQFTSIVP----SLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVR 420
           S LPF  PP FS  P   T+  P    + A  V K     S G++ LNSS DV+  P V+
Sbjct: 361 SSLPFDTPP-FSFFP---TTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVK 420

Query: 421 FNYYSHPDDLARCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAV 480
           FNYYS+  DL+ CV G++K+G++L T  +E  K +DL G   F  LG+ LPEN  +D A 
Sbjct: 421 FNYYSNLTDLSHCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAF 480

Query: 481 EEYCQKTVTTYWHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLG 540
           E +C+++V +YWHYHGGCLVGKV+DD  +V GI+ LRVVDGSTF  +P ++P    +MLG
Sbjct: 481 ETFCRESVASYWHYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLG 540

Query: 541 RYVGLKLLQQRSS 543
           RY+G+++LQ+RS+
Sbjct: 541 RYMGIQILQERSA 547

[Close]

BLAST of CsaV3_2G008170 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 1.1e-146
Identity = 264/551 (47.91%), Postives = 363/551 (65.88%), Query Frame = 0


Query: 1   MKNSMANQILLFTLISIFQLGLI-ASHTIP--NQDTSYMKFVYEASELQESEEYDYIIIG 60
           M   + + +L   L+ +  LG++  S+  P  N+   +M+F+  A++    + YDYII+G
Sbjct: 1   MTKRIDSSLLYTALVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVG 60

Query: 61  GGTAGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISED 120
           GGTAGCPLAATLS  F VLLLERG  P   P+V++  G L      ++  +P Q FISE+
Sbjct: 61  GGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEE 120

Query: 121 GVENLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSP 180
           GV N RGRVLGG S INAGFYSR  + FFE +G+ WD   V ++YEWVE  +V +P L  
Sbjct: 121 GVPNARGRVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRT 180

Query: 181 WQAAFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVAT 240
           WQ A R ALLE GV    GF L H VGTK GGS FD  G RH + +LL      N++VA 
Sbjct: 181 WQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAV 240

Query: 241 QATVQRIIF--------TGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLL 300
            ATV+R++         + +SA GV Y D  G+ H A IR +GE+ILSAGA+GSPQLL L
Sbjct: 241 YATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFL 300

Query: 301 SGVGPKSYLSSLRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHL 360
           SG+GP+SYLS+  +PV LDQPHVG+F+ DNPR   +IV P  + +S  QVVG  +    L
Sbjct: 301 SGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFL 360

Query: 361 QSFASPLPFFAPPSFSLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVR 420
           ++ ++ +PF +P     +    + +   +   + K     S G LRL +S DV+ +P+VR
Sbjct: 361 EAASNVIPFASPLHSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRL-ASTDVRINPVVR 420

Query: 421 FNYYSHPDDLARCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAV 480
           FNY+S P DL RCV G RK+G++L++  M+    ++  GN+RF+F+G  LP +  ND  +
Sbjct: 421 FNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVM 480

Query: 481 EEYCQKTVTTYWHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLG 540
            ++C++TV+T WHYHGG +VGKVVD + KVIG+++LR+VDGSTF++SPGTNP ATLMMLG
Sbjct: 481 ADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLG 540

[Close]

BLAST of CsaV3_2G008170 vs. ExPASy Swiss-Prot
Match: O50048 ((R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 1.9e-143
Identity = 262/548 (47.81%), Postives = 359/548 (65.51%), Query Frame = 0


Query: 1   MKNSMANQILLFTLISIFQLGLIASHTIPN---QDTSYMKFVYEASELQESEEYDYIIIG 60
           +K++M+  ++L   + +  L      ++ N    D SY++FVY+A++ +    YDYII+G
Sbjct: 2   VKSTMSAILVLALHLFVLHLQYSEVQSLANTSAHDFSYLEFVYDANDTELEGTYDYIIVG 61

Query: 61  GGTAGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISED 120
           GGTAGCPLAATLS+ +SVL+LERG  P +YP++L   G +     EDDGQ P + F+S D
Sbjct: 62  GGTAGCPLAATLSANYSVLVLERGTLPTEYPNLLTSDGFIYNLQQEDDGQTPVERFVSGD 121

Query: 121 GVENLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSP 180
           G++N+RGRVLGG SMINAG Y R +  FF   G++WD +LV K Y+WVE+T+V KP    
Sbjct: 122 GIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNKTYDWVEDTIVFKPDFQF 181

Query: 181 WQAAFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVAT 240
           WQ    +A LE G+  D GF L HL GT+  GS FDN G RH + ELLNKG+P NL+VA 
Sbjct: 182 WQNLTGTAFLEVGILPDNGFSLDHLEGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAV 241

Query: 241 QATVQRIIF----TGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVG 300
            A V++IIF    +G++A GV Y+DS G  H AF+R  GE+ILSAG IGSPQLLLLSGVG
Sbjct: 242 HAAVEKIIFSSDSSGVTAIGVIYTDSNGTTHQAFVRGDGEVILSAGPIGSPQLLLLSGVG 301

Query: 301 PKSYLSSLRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFA 360
            +SYL+SL + VV   P+VG+++ DNPR    I+ P  + +S+  V+G        Q   
Sbjct: 302 LESYLTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSI 361

Query: 361 SPLPFFAPPSFSLLP-PQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNY 420
           S LPF   P F   P P +     + A  V K     S G++ L S+ DV+ +P V FNY
Sbjct: 362 SSLPFSTAP-FGFFPNPTYPLPNTTFAHIVNKVPGPLSHGTVLLQSTSDVRVAPNVTFNY 421

Query: 421 YSHPDDLARCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEY 480
           YS+  DLA CV G++K+G+ L +  ++  K +DL G + F  LG+ LPEN  +D A E +
Sbjct: 422 YSNTTDLAHCVSGMKKIGEFLSSDALKPYKVEDLPGIEGFDILGIPLPENQTDDAAFETF 481

Query: 481 CQKTVTTYWHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYV 540
           C++ V +YWHYHGGCLVG+V+DD+ +V GI+ LRVVDGSTF  +P ++P    +MLGRY+
Sbjct: 482 CREAVASYWHYHGGCLVGEVLDDDFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYM 541

[Close]

BLAST of CsaV3_2G008170 vs. ExPASy TrEMBL
Match: A0A0A0LK79 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055050 PE=3 SV=1)

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 542/542 (100.00%), Postives = 542/542 (100.00%), Query Frame = 0


Query: 1   MKNSMANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGT 60
           MKNSMANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGT
Sbjct: 1   MKNSMANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGT 60

Query: 61  AGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVE 120
           AGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVE
Sbjct: 61  AGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVE 120

Query: 121 NLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQA 180
           NLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQA
Sbjct: 121 NLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQA 180

Query: 181 AFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQAT 240
           AFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQAT
Sbjct: 181 AFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQAT 240

Query: 241 VQRIIFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSS 300
           VQRIIFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSS
Sbjct: 241 VQRIIFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSS 300

Query: 301 LRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFA 360
           LRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFA
Sbjct: 301 LRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFA 360

Query: 361 PPSFSLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYYSHPDDLA 420
           PPSFSLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYYSHPDDLA
Sbjct: 361 PPSFSLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYYSHPDDLA 420

Query: 421 RCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTY 480
           RCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTY
Sbjct: 421 RCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTY 480

Query: 481 WHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKLLQQR 540
           WHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKLLQQR
Sbjct: 481 WHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKLLQQR 540

Query: 541 SS 543
           SS
Sbjct: 541 SS 542

[Close]

BLAST of CsaV3_2G008170 vs. ExPASy TrEMBL
Match: A0A5D3CL00 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00890 PE=3 SV=1)

HSP 1 Score: 1035.8 bits (2677), Expect = 6.4e-299
Identity = 513/538 (95.35%), Postives = 530/538 (98.51%), Query Frame = 0


Query: 5   MANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGTAGCP 64
           MAN ILLFTLISIFQLG+I+SH IPNQDTSYMKFV+EASELQESEEYDYIIIGGGTAGCP
Sbjct: 1   MANHILLFTLISIFQLGVISSHPIPNQDTSYMKFVHEASELQESEEYDYIIIGGGTAGCP 60

Query: 65  LAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRG 124
           LAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRG
Sbjct: 61  LAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRG 120

Query: 125 RVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQAAFRS 184
           RVLGGGSMINAGFYSRGHR+FFETAGVDWD ELVEKAYEWVEETVVSKPSLSPWQAAFRS
Sbjct: 121 RVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYEWVEETVVSKPSLSPWQAAFRS 180

Query: 185 ALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQATVQRI 244
           ALLEGGVGHD GFDL+HLVGTKTGGSIFDNKGNRHGAVELLNKGEP+N KVATQATVQRI
Sbjct: 181 ALLEGGVGHDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKGEPENFKVATQATVQRI 240

Query: 245 IFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSSLRLP 304
           IFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKS L SL+LP
Sbjct: 241 IFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSQLKSLKLP 300

Query: 305 VVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFAPPSF 364
           VVLDQPHVGEFMSDNPRF+PTIVLPFQ+V+SSAQVVGTLD+NIHLQ+FASPLPFFAPPSF
Sbjct: 301 VVLDQPHVGEFMSDNPRFTPTIVLPFQVVASSAQVVGTLDNNIHLQAFASPLPFFAPPSF 360

Query: 365 SLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVR 424
           SLLPPQ TSIVPSLAIFVGKFSDV+SEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVR
Sbjct: 361 SLLPPQSTSIVPSLAIFVGKFSDVYSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVR 420

Query: 425 GVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTYWHYH 484
           GVRKVGDLL+TPTMEKIKTQDLEGNKRFQFLGLSLPENLLND+AVEEYCQKTVTTYWHYH
Sbjct: 421 GVRKVGDLLRTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDSAVEEYCQKTVTTYWHYH 480

Query: 485 GGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKLLQQRSS 543
           GGCLVGKVVDDNHKVIGI+NLRVVDGSTFSVSPGTNPMATLMMLGRYVGLK+LQQRSS
Sbjct: 481 GGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKVLQQRSS 538

[Close]

BLAST of CsaV3_2G008170 vs. ExPASy TrEMBL
Match: A0A1S3BSP4 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493073 PE=3 SV=1)

HSP 1 Score: 1035.8 bits (2677), Expect = 6.4e-299
Identity = 513/538 (95.35%), Postives = 530/538 (98.51%), Query Frame = 0


Query: 5   MANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGTAGCP 64
           MAN ILLFTLISIFQLG+I+SH IPNQDTSYMKFV+EASELQESEEYDYIIIGGGTAGCP
Sbjct: 1   MANHILLFTLISIFQLGVISSHPIPNQDTSYMKFVHEASELQESEEYDYIIIGGGTAGCP 60

Query: 65  LAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRG 124
           LAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRG
Sbjct: 61  LAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRG 120

Query: 125 RVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQAAFRS 184
           RVLGGGSMINAGFYSRGHR+FFETAGVDWD ELVEKAYEWVEETVVSKPSLSPWQAAFRS
Sbjct: 121 RVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYEWVEETVVSKPSLSPWQAAFRS 180

Query: 185 ALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQATVQRI 244
           ALLEGGVGHD GFDL+HLVGTKTGGSIFDNKGNRHGAVELLNKGEP+N KVATQATVQRI
Sbjct: 181 ALLEGGVGHDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKGEPENFKVATQATVQRI 240

Query: 245 IFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSSLRLP 304
           IFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKS L SL+LP
Sbjct: 241 IFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSQLKSLKLP 300

Query: 305 VVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFAPPSF 364
           VVLDQPHVGEFMSDNPRF+PTIVLPFQ+V+SSAQVVGTLD+NIHLQ+FASPLPFFAPPSF
Sbjct: 301 VVLDQPHVGEFMSDNPRFTPTIVLPFQVVASSAQVVGTLDNNIHLQAFASPLPFFAPPSF 360

Query: 365 SLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVR 424
           SLLPPQ TSIVPSLAIFVGKFSDV+SEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVR
Sbjct: 361 SLLPPQSTSIVPSLAIFVGKFSDVYSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVR 420

Query: 425 GVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTYWHYH 484
           GVRKVGDLL+TPTMEKIKTQDLEGNKRFQFLGLSLPENLLND+AVEEYCQKTVTTYWHYH
Sbjct: 421 GVRKVGDLLRTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDSAVEEYCQKTVTTYWHYH 480

Query: 485 GGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKLLQQRSS 543
           GGCLVGKVVDDNHKVIGI+NLRVVDGSTFSVSPGTNPMATLMMLGRYVGLK+LQQRSS
Sbjct: 481 GGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKVLQQRSS 538

[Close]

BLAST of CsaV3_2G008170 vs. ExPASy TrEMBL
Match: A0A6J1K4X8 ((R)-mandelonitrile lyase 1-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111492330 PE=3 SV=1)

HSP 1 Score: 875.5 bits (2261), Expect = 1.1e-250
Identity = 431/542 (79.52%), Postives = 479/542 (88.38%), Query Frame = 0


Query: 1   MKNSMANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGT 60
           M+ SMA  IL   LIS FQ G I+S TIP+QD SYMK VY A +L E +EYDYII+GGG 
Sbjct: 1   MERSMAT-ILFLILISTFQFGAISSTTIPDQDDSYMKLVYNAGDLPEKQEYDYIIVGGGA 60

Query: 61  AGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVE 120
           AGCPLAATLSSKF+VLLLERG++PNKYPSVLNEQGLLNAF+A+DDGQNPFQ F SEDGVE
Sbjct: 61  AGCPLAATLSSKFNVLLLERGSEPNKYPSVLNEQGLLNAFLAQDDGQNPFQRFTSEDGVE 120

Query: 121 NLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQA 180
           N+RGR+LGGG+M+NAGFYSRGHR FFETAGV+WD E+VE AY+WVEETVVSKP L+ WQ+
Sbjct: 121 NIRGRILGGGTMVNAGFYSRGHRQFFETAGVNWDMEMVENAYQWVEETVVSKPILNAWQS 180

Query: 181 AFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQAT 240
           AF+SALLE GVG D GF+L HL+GTKTGGSIFD+KGNRHGAVELLNK EPKNLKVA  AT
Sbjct: 181 AFKSALLEAGVGPDNGFNLTHLIGTKTGGSIFDSKGNRHGAVELLNKAEPKNLKVAVNAT 240

Query: 241 VQRIIFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSS 300
           V+RI+F GLSA+GVSYSDSKGK+HTAFIRKKGEI LSAGAIGSP LLL SGVGPKS+LSS
Sbjct: 241 VRRILFNGLSANGVSYSDSKGKIHTAFIRKKGEIFLSAGAIGSPLLLLQSGVGPKSHLSS 300

Query: 301 LRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFA 360
           L+LPVV  QP+VGEFMSDNPRF  TIVLPFQL SSS +VVGTL  N+HLQ+FASP PF A
Sbjct: 301 LKLPVVHHQPYVGEFMSDNPRFGATIVLPFQLASSSVKVVGTLQDNVHLQAFASPAPFLA 360

Query: 361 PPSFSLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYYSHPDDLA 420
           PP+FSLLPPQ TSI PSL IFVGKFS+VHSEG LRLNS+ D K + IVRFNYYSHPDDLA
Sbjct: 361 PPTFSLLPPQATSINPSLVIFVGKFSEVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLA 420

Query: 421 RCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTY 480
           RCV GVRKVGDLLKT TMEKIKTQDLEGNK FQF+G+ LPENL N ++VEEYC+ TVTTY
Sbjct: 421 RCVEGVRKVGDLLKTQTMEKIKTQDLEGNKGFQFIGVPLPENLWNVSSVEEYCRGTVTTY 480

Query: 481 WHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKLLQQR 540
           WHYHGGCLVGKVVD +++VIG+ NLRVVDGSTFS SPGTNPMATLMMLGRYVGLK+LQQR
Sbjct: 481 WHYHGGCLVGKVVDGDYRVIGMKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQR 540

Query: 541 SS 543
           SS
Sbjct: 541 SS 541

[Close]

BLAST of CsaV3_2G008170 vs. ExPASy TrEMBL
Match: A0A6J1EP46 ((R)-mandelonitrile lyase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436113 PE=3 SV=1)

HSP 1 Score: 871.7 bits (2251), Expect = 1.6e-249
Identity = 428/542 (78.97%), Postives = 479/542 (88.38%), Query Frame = 0


Query: 1   MKNSMANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGT 60
           M+ S+A  IL   LIS FQ G I+S+TIP+QD SYMKFV+ A +L E +EYDYIIIGGG 
Sbjct: 1   MERSIAT-ILFLILISTFQFGAISSNTIPDQDVSYMKFVHNAGDLPEKQEYDYIIIGGGA 60

Query: 61  AGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVE 120
           AGCPLAATLSSKF VLLLERG++PNKYPSVLNEQGLLNAF+A+DDGQNPFQ F SEDGVE
Sbjct: 61  AGCPLAATLSSKFKVLLLERGSEPNKYPSVLNEQGLLNAFLAQDDGQNPFQRFTSEDGVE 120

Query: 121 NLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQA 180
           N+RGR+LGG +M+NAGFYSRGHR FFETAGV+WD E+VE AY+WVEETVVSKP L+ WQ+
Sbjct: 121 NIRGRILGGSTMVNAGFYSRGHRQFFETAGVNWDMEMVENAYQWVEETVVSKPILNAWQS 180

Query: 181 AFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQAT 240
           AF+SALLE GVG D GF+L HL+GTKTGGSIFD KGNRHGAVELLNK EP NLKVA  AT
Sbjct: 181 AFKSALLEAGVGPDNGFNLTHLIGTKTGGSIFDGKGNRHGAVELLNKAEPMNLKVAVNAT 240

Query: 241 VQRIIFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSS 300
           V++I+F GLSA+GVSYSDSKGK+HTAFIRKKGEI LSAGAIGSP LLL SGVGPKS+LSS
Sbjct: 241 VRQILFNGLSANGVSYSDSKGKIHTAFIRKKGEIFLSAGAIGSPLLLLQSGVGPKSHLSS 300

Query: 301 LRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFA 360
           L+LPVV  QP+VGEFMSDNPRF  TIVLPFQL SSS +VVGTL  N+HLQ+FASP PF A
Sbjct: 301 LKLPVVHHQPYVGEFMSDNPRFGATIVLPFQLASSSVKVVGTLQDNVHLQAFASPAPFLA 360

Query: 361 PPSFSLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYYSHPDDLA 420
           PP+FSLLPPQ TSI PSL IFVGKFS+VHSEG LRLNS+ D K + IVRFNYYSHPDDLA
Sbjct: 361 PPTFSLLPPQATSINPSLVIFVGKFSEVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLA 420

Query: 421 RCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTY 480
           RCV GVRKVGDLLKT TMEKIKTQDLEGNK FQF+G+ LPENL +D++VEEYC+KTVTTY
Sbjct: 421 RCVEGVRKVGDLLKTQTMEKIKTQDLEGNKGFQFMGVPLPENLGDDSSVEEYCRKTVTTY 480

Query: 481 WHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKLLQQR 540
           WHYHGGCLVGKVVD +++VIG+ NLRVVDGSTFS SPGTNPMATLMMLGRYVGLK+LQ+R
Sbjct: 481 WHYHGGCLVGKVVDGDYRVIGMKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQER 540

Query: 541 SS 543
           SS
Sbjct: 541 SS 541

[Close]

BLAST of CsaV3_2G008170 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 521.5 bits (1342), Expect = 7.8e-148
Identity = 264/551 (47.91%), Postives = 363/551 (65.88%), Query Frame = 0


Query: 1   MKNSMANQILLFTLISIFQLGLI-ASHTIP--NQDTSYMKFVYEASELQESEEYDYIIIG 60
           M   + + +L   L+ +  LG++  S+  P  N+   +M+F+  A++    + YDYII+G
Sbjct: 1   MTKRIDSSLLYTALVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVG 60

Query: 61  GGTAGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISED 120
           GGTAGCPLAATLS  F VLLLERG  P   P+V++  G L      ++  +P Q FISE+
Sbjct: 61  GGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEE 120

Query: 121 GVENLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSP 180
           GV N RGRVLGG S INAGFYSR  + FFE +G+ WD   V ++YEWVE  +V +P L  
Sbjct: 121 GVPNARGRVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRT 180

Query: 181 WQAAFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVAT 240
           WQ A R ALLE GV    GF L H VGTK GGS FD  G RH + +LL      N++VA 
Sbjct: 181 WQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAV 240

Query: 241 QATVQRIIF--------TGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLL 300
            ATV+R++         + +SA GV Y D  G+ H A IR +GE+ILSAGA+GSPQLL L
Sbjct: 241 YATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFL 300

Query: 301 SGVGPKSYLSSLRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHL 360
           SG+GP+SYLS+  +PV LDQPHVG+F+ DNPR   +IV P  + +S  QVVG  +    L
Sbjct: 301 SGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFL 360

Query: 361 QSFASPLPFFAPPSFSLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVR 420
           ++ ++ +PF +P     +    + +   +   + K     S G LRL +S DV+ +P+VR
Sbjct: 361 EAASNVIPFASPLHSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRL-ASTDVRINPVVR 420

Query: 421 FNYYSHPDDLARCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAV 480
           FNY+S P DL RCV G RK+G++L++  M+    ++  GN+RF+F+G  LP +  ND  +
Sbjct: 421 FNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVM 480

Query: 481 EEYCQKTVTTYWHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLG 540
            ++C++TV+T WHYHGG +VGKVVD + KVIG+++LR+VDGSTF++SPGTNP ATLMMLG
Sbjct: 481 ADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLG 540

[Close]

BLAST of CsaV3_2G008170 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 408.7 bits (1049), Expect = 7.4e-114
Identity = 241/572 (42.13%), Postives = 329/572 (57.52%), Query Frame = 0


Query: 11  LFTLISIF-QLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGTAGCPLAATL 70
           LF  +S+F    + +S   PN       F+ +A+    +  YDYIIIGGGTAGCPLAATL
Sbjct: 10  LFVALSLFLHSPICSSDKAPN-----YSFMRDATGSPTTSYYDYIIIGGGTAGCPLAATL 69

Query: 71  SSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRGRVLGG 130
           S   SVLLLERG+ P   P++        A +++    +P Q F+SEDGV N R RVLGG
Sbjct: 70  SQNASVLLLERGDSPYNNPNI-TRLSAFGAALSDLSESSPSQRFVSEDGVINARARVLGG 129

Query: 131 GSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQAAFRSALLEG 190
           GS +NAGFY+R    +    G  WD  L  ++Y+WVE  V  +P +  WQ A R  LLE 
Sbjct: 130 GSALNAGFYTRAGTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEA 189

Query: 191 GVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQATVQRIIF--- 250
           G+  + GF   H+ GTK GG+IFD  GNRH A +LL   +PK + V   ATV RI+F   
Sbjct: 190 GIVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTR 249

Query: 251 --TGLSASGVSYSDSKGKLHTAFIRK--KGEIILSAGAIGSPQLLLLSGVGPKSYLSSLR 310
             T   A+GV Y D  G+ H A++++    EIILSAG +GSPQLL+LSGVGP + L +  
Sbjct: 250 GTTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQN 309

Query: 311 LPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQS----------- 370
           + VV+DQPHVG+ M DNP  +  +  P  +  S  +VVG      ++++           
Sbjct: 310 ITVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGG 369

Query: 371 ----FASPLPFFA--PPSFSLLPPQFTSIVPSLAIFVG-----KFSDVHSEGSLRLNSSI 430
                +S   ++A   P  +LL     + + S   F G     K     S G L L +  
Sbjct: 370 GSSGSSSTRDYYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLELKTR- 429

Query: 431 DVKESPIVRFNYYSHPDDLARCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQF---LGL 490
           + K++PIV FNY+ HPDDL RCVRG++ +  ++++    + K  D+     F++   L  
Sbjct: 430 NPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADVS----FEYLLNLTA 489

Query: 491 SLPENLLN---------DTAVEEYCQKTVTTYWHYHGGCLVGKVVDDNHKVIGIDNLRVV 541
           S P NL             + EE+CQ TVTT WHYHGGC+VG+VVD ++KVIGID LRV+
Sbjct: 490 STPVNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVI 549

[Close]

BLAST of CsaV3_2G008170 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 393.3 bits (1009), Expect = 3.2e-109
Identity = 233/539 (43.23%), Postives = 311/539 (57.70%), Query Frame = 0


Query: 47  ESEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNDP--NKYPSVLNEQGLLNAFVAED 106
           +   YDYI+IGGGTAGCPLAATLS  FSVL+LERG  P  N   S L      +  +A+ 
Sbjct: 59  QDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN---FHIGLADI 118

Query: 107 DGQNPFQHFISEDGVENLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEW 166
              +  Q F+S DGV N R RVLGGGS INAGFYSR    F + AG  WD +LV+++Y W
Sbjct: 119 SASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPW 178

Query: 167 VEETVVSKPSLSPWQAAFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVEL 226
           VE  +V +P L+ WQ A R +LLE GV    GF   H+ GTK GG+IFD  G RH A EL
Sbjct: 179 VEREIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAEL 238

Query: 227 LNKGEPKNLKVATQATVQRIIF----TGLSASGVSYSDSKGKLHTAFI--RKKGEIILSA 286
           L    P+ L+V   ATVQ+I+F    T    +GV + D KG  H A +  RK  E+ILS+
Sbjct: 239 LAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSS 298

Query: 287 GAIGSPQLLLLSGVGPKSYLSSLRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQ 346
           GAIGSPQ+L+LSG+GPK  L  L++PVVL+  HVG+ M+DNP  +  +     +  S  Q
Sbjct: 299 GAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQ 358

Query: 347 VVGTLDHNIHLQSFASPLPFFAPPS---------------FSLLP-----PQFTSIVPSL 406
            VG     +++++      F   P                FS +P     P+ T    + 
Sbjct: 359 TVGITKMGVYVEASTG---FGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITR 418

Query: 407 -----------AIFVGKFSDVHSEGSLRL-NSSIDVKESPIVRFNYYSHPDDLARCVRGV 466
                      +  + K +   S G L L N+++D  ++P V FNY+ HP DL RCV  +
Sbjct: 419 NKYQLHEAFNGSFILEKLAYPISRGHLSLVNTNVD--DNPSVTFNYFKHPVDLQRCVEAI 478

Query: 467 RKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENL----LNDT-AVEEYCQKTVTTYW 526
           R V  ++ +         D +   +   L +    NL    LNDT ++ ++C+ TV T W
Sbjct: 479 RLVSKVVTSNRFLNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIW 538

Query: 527 HYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKLLQQR 541
           HYHGGCLVGKVV  N KV+G+D LRV+DGSTF  SPGTNP AT+MM+GRY+G+K+L++R
Sbjct: 539 HYHGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRER 587

[Close]

BLAST of CsaV3_2G008170 vs. TAIR 10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 379.4 bits (973), Expect = 4.8e-105
Identity = 234/566 (41.34%), Postives = 322/566 (56.89%), Query Frame = 0


Query: 12  FTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGTAGCPLAATLSS 71
           F L+++F    I   +  + +    +F+ +A+   +   +DYIIIGGGTAGC LAATLS 
Sbjct: 11  FILVAVF----IFHDSCYSDEAGNYRFMKDATLAPKLSHFDYIIIGGGTAGCALAATLSQ 70

Query: 72  KFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRGRVLGGGS 131
             +VL+LERG  P   P+  +     N  +      +  Q FISEDGV N R RVLGGG+
Sbjct: 71  NATVLVLERGGSPYDDPAATDIGNFANTLL-NITPNSWSQLFISEDGVFNSRARVLGGGT 130

Query: 132 MINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQAAFRSALLEGGV 191
           +INAGFYSR   DF   AG  W+R+ VE AYEWVE+ VV +P ++ WQ+AFR  LLE GV
Sbjct: 131 VINAGFYSRAEEDFVAEAG--WERDEVEAAYEWVEKKVVFEPPVNKWQSAFRDGLLEAGV 190

Query: 192 GHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQATVQRIIFT---- 251
               GF   H+VGTK GG+IFD  G+RH A  LL    P  + V   A+V +I+FT    
Sbjct: 191 TPYNGFTYEHIVGTKFGGTIFDRDGHRHTAANLLEYANPNMIVVYLHASVHKILFTIKGN 250

Query: 252 -GLSASGVSYSDSKGKLHTAFIRKK----GEIILSAGAIGSPQLLLLSGVGPKSYLSSLR 311
               A GV + D+ G  + A +  +     E+ILSAGAI SPQLL+LSGVGP ++L++ R
Sbjct: 251 QRPKAYGVIFLDANGVSYKAELATQDSTMSEVILSAGAIASPQLLMLSGVGPAAHLAAYR 310

Query: 312 L-PVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQ-SFASPLPFFA 371
           + PV++DQP VG+ M DNP     I  P  +  S  Q VG      +++   A  L    
Sbjct: 311 VNPVIVDQPMVGQGMGDNPMNPVFIPSPEPVEVSLVQAVGITKFGSYIEGGSALSLSISL 370

Query: 372 PPSF----------SLLPPQFTS-----------IVPSLAIFVGKFSDVHSEGSLRLNSS 431
             SF          + LP Q  S           +     + + K +   S G L L ++
Sbjct: 371 TRSFFDGVLNLLKKTKLPTQSISKFFKSLDLTLNVTTKAGVIIQKVNGPLSRGHLELRNT 430

Query: 432 IDVKESPIVRFNYYSHPDDLARCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSL 491
            +  ++P V FNY+  P+DL +CV G+  +  ++ +    K K   L   +    L L+L
Sbjct: 431 -NPDDNPSVTFNYFKDPEDLNKCVEGLSTIIKVIDSKGYSKYK-YPLASARGLLNLILAL 490

Query: 492 PENL-----LNDTAVEEYCQKTVTTYWHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFS 541
           P NL      +   +E+YC  TV T +HYHGGC VGKVVD+N+KV+G+D LR++DGSTF 
Sbjct: 491 PTNLRPRHITSTFDLEQYCIDTVMTIYHYHGGCQVGKVVDNNYKVLGVDALRIIDGSTFL 550

[Close]

BLAST of CsaV3_2G008170 vs. TAIR 10
Match: AT1G72970.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 374.0 bits (959), Expect = 2.0e-103
Identity = 226/535 (42.24%), Postives = 304/535 (56.82%), Query Frame = 0


Query: 47  ESEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNDP--NKYPSVLNEQGLLNAFVAED 106
           +   YDYI+IGGGTAGCPLAATLS  FSVL+LERG  P  N   S L      +  +A+ 
Sbjct: 59  QDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN---FHIGLADI 118

Query: 107 DGQNPFQHFISEDGVENLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEW 166
              +  Q F+S DGV N R RVLGGGS INAGFYSR    F + AG  WD +LV+++Y W
Sbjct: 119 SASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPW 178

Query: 167 VEETVVSKPSLSPWQAAFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVEL 226
           VE  +V +P L+ WQ A R +LLE GV    GF   H+ GTK GG+IFD  G RH A EL
Sbjct: 179 VEREIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAEL 238

Query: 227 LNKGEPKNLKVATQATVQRIIF----TGLSASGVSYSDSKGKLHTAFI--RKKGEIILSA 286
           L    P+ L+V   ATVQ+I+F    T    +GV + D KG  H A +  RK  E+ILS+
Sbjct: 239 LAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSS 298

Query: 287 GAIGSPQLLLLSGVGPKSYLSSLRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQ 346
           GAIGSPQ+L+LSG+GPK  L  L++PVVL+  HVG+ M+DNP  +  +     +  S  Q
Sbjct: 299 GAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQ 358

Query: 347 VVGTLDHNIHLQSFASPLPFFAPPS---------------FSLLP-----PQFTSIVPSL 406
            VG     +++++      F   P                FS +P     P+ T    + 
Sbjct: 359 TVGITKMGVYVEASTG---FGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITR 418

Query: 407 -----------AIFVGKFSDVHSEGSLRL-NSSIDVKESPIVRFNYYSHPDDLARCVRGV 466
                      +  + K +   S G L L N+++D  ++P V FNY+ HP D        
Sbjct: 419 NKYQLHEAFNGSFILEKLAYPISRGHLSLVNTNVD--DNPSVTFNYFKHPCD-------- 478

Query: 467 RKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDT-AVEEYCQKTVTTYWHYHG 526
                      + K+ +  ++ N       ++L    LNDT ++ ++C+ TV T WHYHG
Sbjct: 479 --------KQNVHKMLSLSVKAN-------INLRPKQLNDTKSMAQFCKDTVVTIWHYHG 538

Query: 527 GCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKLLQQR 541
           GCLVGKVV  N KV+G+D LRV+DGSTF  SPGTNP AT+MM+GRY+G+K+L++R
Sbjct: 539 GCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRER 560

The following BLAST results are available for this feature:

BLAST of CsaV3_2G008170 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031736478.1	0.0e+00	100.00	(R)-mandelonitrile lyase 1-like [Cucumis sativus] >KGN61117.1 hypothetical prote...	[more]
XP_008451922.1	1.3e-298	95.35	PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo] >KAA0067298.1 (R)-mand...	[more]
XP_038889699.1	4.4e-278	89.30	(R)-mandelonitrile lyase 1-like [Benincasa hispida]	[more]
XP_038889315.1	3.9e-258	81.18	(R)-mandelonitrile lyase 1-like [Benincasa hispida]	[more]
XP_038889314.1	3.1e-255	81.18	(R)-mandelonitrile lyase 1-like [Benincasa hispida]	[more]

BLAST of CsaV3_2G008170 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52706	4.9e-155	50.82	(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1	[more]
Q945K2	7.4e-151	49.73	(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1	[more]
P52707	2.6e-148	48.82	(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1	[more]
Q9SSM2	1.1e-146	47.91	(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ...	[more]
O50048	1.9e-143	47.81	(R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1	[more]

BLAST of CsaV3_2G008170 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LK79	0.0e+00	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055050 PE=3 SV=1	[more]
A0A5D3CL00	6.4e-299	95.35	(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3BSP4	6.4e-299	95.35	(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493073 PE=3 SV=...	[more]
A0A6J1K4X8	1.1e-250	79.52	(R)-mandelonitrile lyase 1-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1EP46	1.6e-249	78.97	(R)-mandelonitrile lyase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]

BLAST of CsaV3_2G008170 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73050.1	7.8e-148	47.91	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G12570.1	7.4e-114	42.13	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G72970.1	3.2e-109	43.23	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT3G56060.1	4.8e-105	41.34	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G72970.2	2.0e-103	42.24	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PFAM	PF00732	GMC_oxred_N	coord: 51..319 e-value: 5.0E-32 score: 111.5
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PROSITE	PS00623	GMC_OXRED_1	coord: 124..147
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PROSITE	PS00624	GMC_OXRED_2	coord: 279..293
IPR007867	Glucose-methanol-choline oxidoreductase, C-terminal	PFAM	PF05199	GMC_oxred_C	coord: 389..530 e-value: 1.1E-28 score: 100.6
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 51..529 e-value: 3.0E-166 score: 556.1
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 35..539
None	No IPR available	GENE3D	3.30.410.40		coord: 171..479 e-value: 3.0E-166 score: 556.1
None	No IPR available	PANTHER	PTHR45968	OSJNBA0019K04.7 PROTEIN	coord: 2..541
None	No IPR available	PANTHER	PTHR45968:SF23	(R)-MANDELONITRILE LYASE 4-RELATED	coord: 2..541
None	No IPR available	SUPERFAMILY	54373	FAD-linked reductases, C-terminal domain	coord: 389..486
IPR012132	Glucose-methanol-choline oxidoreductase	PIRSF	PIRSF000137	Alcohol_oxidase	coord: 356..542 e-value: 1.8E-30 score: 104.2 coord: 35..351 e-value: 4.2E-58 score: 195.5

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_2G008170.1	CsaV3_2G008170.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016614	oxidoreductase activity, acting on CH-OH group of donors