CsaV3_2G007090 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_2G007090
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Descriptionnicotianamine synthase-like
Locationchr2: 3536310 .. 3538376 (+)
RNA-Seq ExpressionCsaV3_2G007090
SyntenyCsaV3_2G007090
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAGAAGAAACCCACCAATATCCTTTCACCAAAAAAAAGAAGAAAAACTCCCCCCCTTTGGCTTAGCCATGGAATTTGAACAACAACAAGAAGCACTTCTGTTGGAAAAAGTCTGTGCCCTTTACAACCAAATCTCAAGCCTTTCTAGCCTTAAGCCTTGTAAAAATGTCGACACTCTTTTCACTCAACTCGTCCTCACGTGCTCACAGCACCCACCTCCTCCTATTGGCTTTGATATTGCTTCTTTGTCTCAGCCACTTCGAGCCATGAGGGCTCATCTCATTCAACTTTGTGCTCAAGCTGAGGCTCTTCTTGAACTTCATTTCTCTTCCCTTTTGGCTTCTTCTTTTCATCATCCTATTTCTAATTTGTCTATCTTCCCTTACTACTCTAATTACTTGAAGCTTTCCCTTCTTGAGTTTGATATTCTTCGATCTCATTCTCGTCGTATCCCCGATAAAGTTGCCTTTGTTGGCTCCGGTCCCCTCCCGCTCTCCTCCATTGTCTTGGCGTCTATTCATCTTAAGGGTAACCGGTAGTTGTTTGAATTATTAGTTTCTAACTATATATACTTGTAGGTGTGTTGTTTTATGGTGCATGCTTATGGGTTGGAATTATTGGATAAACATAACGACGATTCATTTTAAACTTAATTGATAATGAAATGAGTAATTCCTAAACATTATGAGTTTGGTTTCTCCATTTTTTTAATATGAGATCGTCAGTATGTTCTGAAGATGTTTTTTCAGTTTACCATTATTCTTAGGAGTCAATTTTGTTTTTAACCAAATATTTATTTGAATTTTATTGACTCTTTTACCATGTTAGAAAAGAGAAATTTCCAACTCAAACTTAATTGCTAATAGTGGAATAAGTGACTTGTGTATATCATGAGATCTCCTCATCTTTTCAATGTAAGGTTCTGGATAGACATGCAAAGTTTAGGTTTAATAGTATATTTTATCACTTGATTTGTAGATATTTCGTTTGTGATGACAATATAGAGATTTCAAATATATACATGAACCTTATTTTGTTAGTATTTGAGGTGGAGGAGAGTTAACATTTTAACTTTACTTCTTGTTTGGTTCAAAAAGTTTTTAAATGACTAACTGTGGCCCATGACTTATTAACATCAATTCTATGACTCCTTGTTAACTTGCATGATAGGTCCCTCTACCAAACAATGTATCAAACTAAACAACTCCGGACTAAACAATTTCACTCTATGTTCGATTATCATAAATTGTTTTAATTACTTTCATCTTCACCTCTTTATGAATAGGTACTATTTTCCACAACTTTGACATTGATCCAACAGCAAACACCATGGCAAGTCAATTGGTTTGCTCTGACCCAGACTTATCACAAAGAATGATATTTCACACAAAAGATGTAATGGAAGTAACAAAGGGATTAAAAGATTATGAAGTAGTGTTCTTAGCAGCATTAGTTGGATTAGGAGAGGAAGAAAAAGGAAGAGTTTTAAAGCATTTGGGGAAACATATGGCAGCTGGTTCATATTTGATGCTAAGAAGTGCTCATGGAGCAAGAGCATTTTTGTACCCTGTTGTTGATATTTGTACTGTTGAAGCTTCTGGATTTGAGATTTTGTCTGTGTTCCATCCCACCGATGAAGTTATTAACTCTGTGGTTATTGCAAGGAAGAAAGTCATGTTCGATCAAAGCTCGGACGAGGACGAGGACGAGGACGAGAAGCAAGGTATTTTAGTCAATACTTCTTCTATTGTGTTGGCTGAGAAATGCTCTGGGTTTAATGGCTTTACTCCCATGATTGAAGAGCCACTTTCTTGAAGTTTTTATCGTTGTATTTGCATTTGATTTTCATTCACCCTACTCTAAATCTATATGTTTCTATGGCAAGTTCCTTTTTTATTTTGTGTCGGTCCAAAGAGTTTTTACCTTCTTTTCTTTACGTATAGATCAAATTGTTAAAGTGAGTTTGACTCAGTGATATTGACATACACTCTATCTCTAGAGGCCAAAAGTTCAATTCTTCACCTCACACTTGTTGTACTCAACAAAAACTATAAATCAAATTTGTT

mRNA sequence

ATGGAATTTGAACAACAACAAGAAGCACTTCTGTTGGAAAAAGTCTGTGCCCTTTACAACCAAATCTCAAGCCTTTCTAGCCTTAAGCCTTGTAAAAATGTCGACACTCTTTTCACTCAACTCGTCCTCACGTGCTCACAGCACCCACCTCCTCCTATTGGCTTTGATATTGCTTCTTTGTCTCAGCCACTTCGAGCCATGAGGGCTCATCTCATTCAACTTTGTGCTCAAGCTGAGGCTCTTCTTGAACTTCATTTCTCTTCCCTTTTGGCTTCTTCTTTTCATCATCCTATTTCTAATTTGTCTATCTTCCCTTACTACTCTAATTACTTGAAGCTTTCCCTTCTTGAGTTTGATATTCTTCGATCTCATTCTCGTCGTATCCCCGATAAAGTTGCCTTTGTTGGCTCCGGTCCCCTCCCGCTCTCCTCCATTGTCTTGGCGTCTATTCATCTTAAGGGTACTATTTTCCACAACTTTGACATTGATCCAACAGCAAACACCATGGCAAGTCAATTGGTTTGCTCTGACCCAGACTTATCACAAAGAATGATATTTCACACAAAAGATGTAATGGAAGTAACAAAGGGATTAAAAGATTATGAAGTAGTGTTCTTAGCAGCATTAGTTGGATTAGGAGAGGAAGAAAAAGGAAGAGTTTTAAAGCATTTGGGGAAACATATGGCAGCTGGTTCATATTTGATGCTAAGAAGTGCTCATGGAGCAAGAGCATTTTTGTACCCTGTTGTTGATATTTGTACTGTTGAAGCTTCTGGATTTGAGATTTTGTCTGTGTTCCATCCCACCGATGAAGTTATTAACTCTGTGGTTATTGCAAGGAAGAAAGTCATGTTCGATCAAAGCTCGGACGAGGACGAGGACGAGGACGAGAAGCAAGGTATTTTAGTCAATACTTCTTCTATTGTGTTGGCTGAGAAATGCTCTGGGTTTAATGGCTTTACTCCCATGATTGAAGAGCCACTTTCTTGA

Coding sequence (CDS)

ATGGAATTTGAACAACAACAAGAAGCACTTCTGTTGGAAAAAGTCTGTGCCCTTTACAACCAAATCTCAAGCCTTTCTAGCCTTAAGCCTTGTAAAAATGTCGACACTCTTTTCACTCAACTCGTCCTCACGTGCTCACAGCACCCACCTCCTCCTATTGGCTTTGATATTGCTTCTTTGTCTCAGCCACTTCGAGCCATGAGGGCTCATCTCATTCAACTTTGTGCTCAAGCTGAGGCTCTTCTTGAACTTCATTTCTCTTCCCTTTTGGCTTCTTCTTTTCATCATCCTATTTCTAATTTGTCTATCTTCCCTTACTACTCTAATTACTTGAAGCTTTCCCTTCTTGAGTTTGATATTCTTCGATCTCATTCTCGTCGTATCCCCGATAAAGTTGCCTTTGTTGGCTCCGGTCCCCTCCCGCTCTCCTCCATTGTCTTGGCGTCTATTCATCTTAAGGGTACTATTTTCCACAACTTTGACATTGATCCAACAGCAAACACCATGGCAAGTCAATTGGTTTGCTCTGACCCAGACTTATCACAAAGAATGATATTTCACACAAAAGATGTAATGGAAGTAACAAAGGGATTAAAAGATTATGAAGTAGTGTTCTTAGCAGCATTAGTTGGATTAGGAGAGGAAGAAAAAGGAAGAGTTTTAAAGCATTTGGGGAAACATATGGCAGCTGGTTCATATTTGATGCTAAGAAGTGCTCATGGAGCAAGAGCATTTTTGTACCCTGTTGTTGATATTTGTACTGTTGAAGCTTCTGGATTTGAGATTTTGTCTGTGTTCCATCCCACCGATGAAGTTATTAACTCTGTGGTTATTGCAAGGAAGAAAGTCATGTTCGATCAAAGCTCGGACGAGGACGAGGACGAGGACGAGAAGCAAGGTATTTTAGTCAATACTTCTTCTATTGTGTTGGCTGAGAAATGCTCTGGGTTTAATGGCTTTACTCCCATGATTGAAGAGCCACTTTCTTGA

Protein sequence

MEFEQQQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASLSQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSSDEDEDEDEKQGILVNTSSIVLAEKCSGFNGFTPMIEEPLS*
Homology
BLAST of CsaV3_2G007090 vs. NCBI nr
Match: XP_004144750.1 (probable nicotianamine synthase 4 [Cucumis sativus] >KGN61000.1 hypothetical protein Csa_021149 [Cucumis sativus])

HSP 1 Score: 644.0 bits (1660), Expect = 6.8e-181
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0

Query: 1   MEFEQQQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASL 60
           MEFEQQQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASL
Sbjct: 1   MEFEQQQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASL 60

Query: 61  SQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDI 120
           SQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDI
Sbjct: 61  SQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDI 120

Query: 121 LRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDL 180
           LRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDL
Sbjct: 121 LRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDL 180

Query: 181 SQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAH 240
           SQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAH
Sbjct: 181 SQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAH 240

Query: 241 GARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSSDEDEDEDEKQG 300
           GARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSSDEDEDEDEKQG
Sbjct: 241 GARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSSDEDEDEDEKQG 300

Query: 301 ILVNTSSIVLAEKCSGFNGFTPMIEEPLS 330
           ILVNTSSIVLAEKCSGFNGFTPMIEEPLS
Sbjct: 301 ILVNTSSIVLAEKCSGFNGFTPMIEEPLS 329

BLAST of CsaV3_2G007090 vs. NCBI nr
Match: KAA0048069.1 (putative nicotianamine synthase 4 isoform X2 [Cucumis melo var. makuwa] >TYJ96467.1 putative nicotianamine synthase 4 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 597.8 bits (1540), Expect = 5.6e-167
Identity = 313/338 (92.60%), Postives = 324/338 (95.86%), Query Frame = 0

Query: 1   MEFEQ----QQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPP-PPIGF 60
           MEFEQ    QQEALLL+KVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQ PP  PIGF
Sbjct: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DIASLSQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120
           D+ASLSQPL+AMRAHLIQLCA+AEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180
           LEFDILRSHSRR+P+KVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180

Query: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLM 240
           SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGL EEEKGRVLKHLGKHMAAGSYLM
Sbjct: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240

Query: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSS----DE 300
           LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKV+FDQSS    DE
Sbjct: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSVEDEDE 300

Query: 301 DEDEDEKQGILVNTSSIVLAEKCSGFNGFTPMIEEPLS 330
           DE+E+EK GILV+TSSIVLAEKCSGFNGFTP+IEE LS
Sbjct: 301 DEEEEEKPGILVDTSSIVLAEKCSGFNGFTPIIEEQLS 338

BLAST of CsaV3_2G007090 vs. NCBI nr
Match: XP_038890564.1 (probable nicotianamine synthase 4 [Benincasa hispida])

HSP 1 Score: 541.2 bits (1393), Expect = 6.2e-150
Identity = 282/333 (84.68%), Postives = 306/333 (91.89%), Query Frame = 0

Query: 1   MEFEQQQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASL 60
           MEFE   EA LL+K+ ALY+QISSL+SL P KNVDTLFTQLVLTCS  PP P GFDIASL
Sbjct: 1   MEFE---EAPLLQKISALYHQISSLTSLNPSKNVDTLFTQLVLTCS--PPAPAGFDIASL 60

Query: 61  SQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDI 120
           SQPL+AMRAHLI+LCA+AEALLELHFSS+LASSF +PISNLSIFPYYSNYLKLSLLEFDI
Sbjct: 61  SQPLQAMRAHLIRLCAKAEALLELHFSSILASSFRNPISNLSIFPYYSNYLKLSLLEFDI 120

Query: 121 LRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDL 180
           LRSH RR+P  +AF+GSGPLPLSSIV+ASIHLKGTIFHNFD+DPTAN+MASQLVCSDPDL
Sbjct: 121 LRSHCRRVPASIAFLGSGPLPLSSIVMASIHLKGTIFHNFDLDPTANSMASQLVCSDPDL 180

Query: 181 SQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAH 240
           SQRMIFHT DVM VT GLKDYEVVFLAALVGL EEEKGRVLKHLGKHMAAG+YLMLRSAH
Sbjct: 181 SQRMIFHTTDVMNVTNGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGAYLMLRSAH 240

Query: 241 GARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSSDEDEDED---- 300
           GARAFLYPVVDIC VEASGFEILSVFHPTDEVINSVVIARKK +FD++S EDED+D    
Sbjct: 241 GARAFLYPVVDICDVEASGFEILSVFHPTDEVINSVVIARKKALFDRNSVEDEDDDDNAA 300

Query: 301 EKQGILVNTSSIVLAEKCSGFNGFTPMIEEPLS 330
           EK+GILV+TSSIVLAEKCSGFNGF+PM+EE LS
Sbjct: 301 EKRGILVDTSSIVLAEKCSGFNGFSPMVEEQLS 328

BLAST of CsaV3_2G007090 vs. NCBI nr
Match: XP_008453482.1 (PREDICTED: probable nicotianamine synthase 4 isoform X2 [Cucumis melo])

HSP 1 Score: 531.6 bits (1368), Expect = 4.9e-147
Identity = 276/291 (94.85%), Postives = 283/291 (97.25%), Query Frame = 0

Query: 1   MEFEQ----QQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPP-PPIGF 60
           MEFEQ    QQEALLL+KVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQ PP  PIGF
Sbjct: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DIASLSQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120
           D+ASLSQPL+AMRAHLIQLCA+AEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180
           LEFDILRSHSRR+P+KVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180

Query: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLM 240
           SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGL EEEKGRVLKHLGKHMAAGSYLM
Sbjct: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240

Query: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFD 287
           LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKV+FD
Sbjct: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 291

BLAST of CsaV3_2G007090 vs. NCBI nr
Match: XP_008453473.1 (PREDICTED: probable nicotianamine synthase 4 isoform X1 [Cucumis melo])

HSP 1 Score: 526.6 bits (1355), Expect = 1.6e-145
Identity = 276/293 (94.20%), Postives = 283/293 (96.59%), Query Frame = 0

Query: 1   MEFEQ----QQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPP-PPIGF 60
           MEFEQ    QQEALLL+KVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQ PP  PIGF
Sbjct: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DIASLSQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120
           D+ASLSQPL+AMRAHLIQLCA+AEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKG--TIFHNFDIDPTANTMASQL 180
           LEFDILRSHSRR+P+KVAFVGSGPLPLSSIVLASIHLKG  TIFHNFDIDPTANTMASQL
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGNRTIFHNFDIDPTANTMASQL 180

Query: 181 VCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSY 240
           VCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGL EEEKGRVLKHLGKHMAAGSY
Sbjct: 181 VCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSY 240

Query: 241 LMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFD 287
           LMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKV+FD
Sbjct: 241 LMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 293

BLAST of CsaV3_2G007090 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 6.4e-89
Identity = 175/326 (53.68%), Postives = 240/326 (73.62%), Query Frame = 0

Query: 7   QEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASLSQPLRA 66
           Q+  L+ K+C LY +IS L +LKPC++VDTLF QLV TC    PP    D+  +S+ ++ 
Sbjct: 5   QDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCI---PPNPNIDVTKMSENIQE 64

Query: 67  MRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSH-S 126
           MR++LI++C +AE  LE HFSS+L S   +P+ +L++FPYY+NYLKLS LEFD+L  + +
Sbjct: 65  MRSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLN 124

Query: 127 RRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMI 186
             +P  VAF+GSGPLPL+S+VLAS HLK +IFHNFDIDP+AN +A++LV SDPDLSQRM 
Sbjct: 125 GFVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMF 184

Query: 187 FHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAF 246
           FHT D+M+VT+ LK ++VVFLAALVG+ ++EK +V++HL KHM+ G+ LMLRSAHG RAF
Sbjct: 185 FHTVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAF 244

Query: 247 LYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSSDEDEDEDEKQGILVNT 306
           LYP+V+ C +E  GFE+LSV+HPTDEVINS+VI+R K+  D +    +  D+   +  N 
Sbjct: 245 LYPIVEPCDLE--GFEVLSVYHPTDEVINSIVISR-KLGEDANGVVHDHIDQASDLACNC 304

Query: 307 SSI--VLAEKCSGFNGFTPMIEEPLS 330
           S I  ++ +K S    F    EE L+
Sbjct: 305 SKIHVIMNKKKSIIEEFAGANEEQLT 324

BLAST of CsaV3_2G007090 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 7.8e-87
Identity = 167/271 (61.62%), Postives = 213/271 (78.60%), Query Frame = 0

Query: 11  LLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASLSQPLRAMRAH 70
           ++EKVC LY QIS L +L P K+V+ LFT LV TC   PP PI  D++ L Q ++ +R+H
Sbjct: 9   VVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCM--PPNPI--DVSKLCQKIQEIRSH 68

Query: 71  LIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSHSRRIPD 130
           LI+LC QAE LLE HFS +L SS+ +P+ +L IFPY+ NY+KLSLLE++IL  ++  IP 
Sbjct: 69  LIKLCGQAEGLLESHFSKIL-SSYENPLQHLHIFPYFDNYIKLSLLEYNILTKNTTNIPK 128

Query: 131 KVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFHTKD 190
           K+AF+GSGPLPL+S+VLA+ HLK T FHN+DID  AN MAS LV +DPD+S RM FHT D
Sbjct: 129 KIAFIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMTFHTAD 188

Query: 191 VMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLYPVV 250
           VM+VT  LKDY+VVFLAALVG+ +E+K +V+ HL K+M+ G+ LMLRSAHGARAFLYPV+
Sbjct: 189 VMDVTCALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAFLYPVL 248

Query: 251 DICTVEASGFEILSVFHPTDEVINSVVIARK 282
           D    +  GFE+LSV+HPTDEVINSV+IARK
Sbjct: 249 D--PRDLRGFEVLSVYHPTDEVINSVIIARK 272

BLAST of CsaV3_2G007090 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 4.3e-85
Identity = 161/277 (58.12%), Postives = 207/277 (74.73%), Query Frame = 0

Query: 7   QEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASLSQPLRA 66
           Q  L+++++  LY+QIS L SLKP KNVDTLF QLV TC    P     D+ ++ + ++ 
Sbjct: 4   QNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCL---PTDTNIDVTNMCEEVKD 63

Query: 67  MRAHLIQLCAQAEALLELHFSSLLAS--SFHHPISNLSIFPYYSNYLKLSLLEFDILRSH 126
           MRA+LI+LC +AE  LE HFS++L S     +P+ +L IFPYYSNYLKL  LEFD+L  H
Sbjct: 64  MRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQH 123

Query: 127 SRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRM 186
           S  +P K+AFVGSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  DPDLS+RM
Sbjct: 124 SSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRM 183

Query: 187 IFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAHGARA 246
           IFHT DV+  T+GL  Y+VVFLAALVG+ +E K + ++HL KHMA G+ LMLRSAH  RA
Sbjct: 184 IFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRA 243

Query: 247 FLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARK 282
           FLYP+VD  + +  GF++L+++HPTD+V+NSVVIARK
Sbjct: 244 FLYPIVD--SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

BLAST of CsaV3_2G007090 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 7.3e-85
Identity = 158/273 (57.88%), Postives = 206/273 (75.46%), Query Frame = 0

Query: 10  LLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASL-SQPLRAMR 69
           L+++++  LYNQIS+L SLKP KNVDTLF QLV TC    P     D+  +  + ++ MR
Sbjct: 7   LVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCL---PTDTNIDVTEIHDEKVKDMR 66

Query: 70  AHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSHSRRI 129
           +HLI+LC +AE  LE HFS++L S   +P+++L IFPYY+NYLKL  LEFD+L  H+  +
Sbjct: 67  SHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHV 126

Query: 130 PDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFHT 189
           P KVAF+GSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  D DLS+RMIFHT
Sbjct: 127 PTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHT 186

Query: 190 KDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLYP 249
            DV+   +GL  Y+VVFLAALVG+ +E K + ++HL KHMA G+ +MLRSAHG RAFLYP
Sbjct: 187 TDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYP 246

Query: 250 VVDICTVEASGFEILSVFHPTDEVINSVVIARK 282
           +VD C  +  GFE+L+++HP+D+V+NSVVIARK
Sbjct: 247 IVDSC--DLKGFEVLTIYHPSDDVVNSVVIARK 274

BLAST of CsaV3_2G007090 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 9.5e-85
Identity = 160/276 (57.97%), Postives = 213/276 (77.17%), Query Frame = 0

Query: 7   QEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASLSQPLRA 66
           Q+  L++ +C LY +IS L SLKP ++V+ LF QLV TC    PP    D+  +   ++ 
Sbjct: 4   QDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCI---PPNPNIDVTKMCDRVQE 63

Query: 67  MRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSH-S 126
           +R +LI++C  AE  LE HFSS+L S   +P+ +L+IFPYY+NYLKL  LEFD+L  + +
Sbjct: 64  IRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLN 123

Query: 127 RRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMI 186
             +P  VAF+GSGPLPL+SIVLAS HLK TIFHNFDIDP+AN++AS LV SDPD+SQRM 
Sbjct: 124 GFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMF 183

Query: 187 FHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAF 246
           FHT D+M+VT+ LK ++VVFLAALVG+ +EEK +V++HL KHMA G+ LMLRSAHG RAF
Sbjct: 184 FHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAF 243

Query: 247 LYPVVDICTVEASGFEILSVFHPTDEVINSVVIARK 282
           LYP+V+ C ++  GFE+LS++HPTD+VINSVVI++K
Sbjct: 244 LYPIVEPCDLQ--GFEVLSIYHPTDDVINSVVISKK 274

BLAST of CsaV3_2G007090 vs. ExPASy TrEMBL
Match: A0A0A0LGI4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034530 PE=3 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 3.3e-181
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0

Query: 1   MEFEQQQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASL 60
           MEFEQQQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASL
Sbjct: 1   MEFEQQQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASL 60

Query: 61  SQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDI 120
           SQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDI
Sbjct: 61  SQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDI 120

Query: 121 LRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDL 180
           LRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDL
Sbjct: 121 LRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDL 180

Query: 181 SQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAH 240
           SQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAH
Sbjct: 181 SQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAH 240

Query: 241 GARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSSDEDEDEDEKQG 300
           GARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSSDEDEDEDEKQG
Sbjct: 241 GARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSSDEDEDEDEKQG 300

Query: 301 ILVNTSSIVLAEKCSGFNGFTPMIEEPLS 330
           ILVNTSSIVLAEKCSGFNGFTPMIEEPLS
Sbjct: 301 ILVNTSSIVLAEKCSGFNGFTPMIEEPLS 329

BLAST of CsaV3_2G007090 vs. ExPASy TrEMBL
Match: A0A5D3BBH0 (Putative nicotianamine synthase 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001110 PE=3 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 2.7e-167
Identity = 313/338 (92.60%), Postives = 324/338 (95.86%), Query Frame = 0

Query: 1   MEFEQ----QQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPP-PPIGF 60
           MEFEQ    QQEALLL+KVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQ PP  PIGF
Sbjct: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DIASLSQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120
           D+ASLSQPL+AMRAHLIQLCA+AEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180
           LEFDILRSHSRR+P+KVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180

Query: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLM 240
           SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGL EEEKGRVLKHLGKHMAAGSYLM
Sbjct: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240

Query: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSS----DE 300
           LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKV+FDQSS    DE
Sbjct: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSVEDEDE 300

Query: 301 DEDEDEKQGILVNTSSIVLAEKCSGFNGFTPMIEEPLS 330
           DE+E+EK GILV+TSSIVLAEKCSGFNGFTP+IEE LS
Sbjct: 301 DEEEEEKPGILVDTSSIVLAEKCSGFNGFTPIIEEQLS 338

BLAST of CsaV3_2G007090 vs. ExPASy TrEMBL
Match: A0A1S3BXI8 (probable nicotianamine synthase 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494174 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.4e-147
Identity = 276/291 (94.85%), Postives = 283/291 (97.25%), Query Frame = 0

Query: 1   MEFEQ----QQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPP-PPIGF 60
           MEFEQ    QQEALLL+KVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQ PP  PIGF
Sbjct: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DIASLSQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120
           D+ASLSQPL+AMRAHLIQLCA+AEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180
           LEFDILRSHSRR+P+KVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180

Query: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLM 240
           SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGL EEEKGRVLKHLGKHMAAGSYLM
Sbjct: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240

Query: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFD 287
           LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKV+FD
Sbjct: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 291

BLAST of CsaV3_2G007090 vs. ExPASy TrEMBL
Match: A0A1S3BWE4 (probable nicotianamine synthase 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494174 PE=3 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 7.7e-146
Identity = 276/293 (94.20%), Postives = 283/293 (96.59%), Query Frame = 0

Query: 1   MEFEQ----QQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPP-PPIGF 60
           MEFEQ    QQEALLL+KVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQ PP  PIGF
Sbjct: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DIASLSQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120
           D+ASLSQPL+AMRAHLIQLCA+AEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKG--TIFHNFDIDPTANTMASQL 180
           LEFDILRSHSRR+P+KVAFVGSGPLPLSSIVLASIHLKG  TIFHNFDIDPTANTMASQL
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGNRTIFHNFDIDPTANTMASQL 180

Query: 181 VCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSY 240
           VCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGL EEEKGRVLKHLGKHMAAGSY
Sbjct: 181 VCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSY 240

Query: 241 LMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFD 287
           LMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKV+FD
Sbjct: 241 LMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 293

BLAST of CsaV3_2G007090 vs. ExPASy TrEMBL
Match: A0A6J1G3L4 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111450477 PE=3 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 9.5e-128
Identity = 250/322 (77.64%), Postives = 275/322 (85.40%), Query Frame = 0

Query: 8   EALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASLSQPLRAM 67
           E  LL +VCALY+QISSL+SLKP KNVDTLFTQLVLTCS   PP  G DIASL QPL+AM
Sbjct: 2   EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCS---PPAPGLDIASLPQPLQAM 61

Query: 68  RAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSHSRR 127
           RAHLI+LCA AEALLE+HFSS+LA SF +PI NLSIFPYYSNYLKLSLLEFDIL SH RR
Sbjct: 62  RAHLIRLCATAEALLEVHFSSILA-SFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRR 121

Query: 128 IPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFH 187
           +P  VAFVGSGPLPLSSIV+AS HLKGTIFHNFDIDP AN+MA QLVCSD DLS+RM+FH
Sbjct: 122 VPSSVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFH 181

Query: 188 TKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLY 247
           T DVMEVT+   +YEVVFLAALVGL EEEKGRVL+HLGKHMA G+YLMLRSAHGARAFLY
Sbjct: 182 TTDVMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLY 241

Query: 248 PVVDICTVEASGFEILSVFHPTDEVINSVVIARK--KVMFDQSSDEDED-EDEKQGILVN 307
           PVVD+  VEASGFEILSVFHPTDEVINSVVIARK   ++ D  +  D D +DEK+ +LVN
Sbjct: 242 PVVDVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDNDTDDEKRAVLVN 301

Query: 308 TSSIVLAEKCSGFNGFTPMIEE 327
           T  I+LAEKCSGF  F PMIEE
Sbjct: 302 T-PIILAEKCSGFKDFGPMIEE 318

BLAST of CsaV3_2G007090 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 328.9 bits (842), Expect = 4.5e-90
Identity = 175/326 (53.68%), Postives = 240/326 (73.62%), Query Frame = 0

Query: 7   QEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASLSQPLRA 66
           Q+  L+ K+C LY +IS L +LKPC++VDTLF QLV TC    PP    D+  +S+ ++ 
Sbjct: 5   QDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCI---PPNPNIDVTKMSENIQE 64

Query: 67  MRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSH-S 126
           MR++LI++C +AE  LE HFSS+L S   +P+ +L++FPYY+NYLKLS LEFD+L  + +
Sbjct: 65  MRSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLN 124

Query: 127 RRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMI 186
             +P  VAF+GSGPLPL+S+VLAS HLK +IFHNFDIDP+AN +A++LV SDPDLSQRM 
Sbjct: 125 GFVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMF 184

Query: 187 FHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAF 246
           FHT D+M+VT+ LK ++VVFLAALVG+ ++EK +V++HL KHM+ G+ LMLRSAHG RAF
Sbjct: 185 FHTVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAF 244

Query: 247 LYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSSDEDEDEDEKQGILVNT 306
           LYP+V+ C +E  GFE+LSV+HPTDEVINS+VI+R K+  D +    +  D+   +  N 
Sbjct: 245 LYPIVEPCDLE--GFEVLSVYHPTDEVINSIVISR-KLGEDANGVVHDHIDQASDLACNC 304

Query: 307 SSI--VLAEKCSGFNGFTPMIEEPLS 330
           S I  ++ +K S    F    EE L+
Sbjct: 305 SKIHVIMNKKKSIIEEFAGANEEQLT 324

BLAST of CsaV3_2G007090 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 316.2 bits (809), Expect = 3.0e-86
Identity = 161/277 (58.12%), Postives = 207/277 (74.73%), Query Frame = 0

Query: 7   QEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASLSQPLRA 66
           Q  L+++++  LY+QIS L SLKP KNVDTLF QLV TC    P     D+ ++ + ++ 
Sbjct: 4   QNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCL---PTDTNIDVTNMCEEVKD 63

Query: 67  MRAHLIQLCAQAEALLELHFSSLLAS--SFHHPISNLSIFPYYSNYLKLSLLEFDILRSH 126
           MRA+LI+LC +AE  LE HFS++L S     +P+ +L IFPYYSNYLKL  LEFD+L  H
Sbjct: 64  MRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQH 123

Query: 127 SRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRM 186
           S  +P K+AFVGSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  DPDLS+RM
Sbjct: 124 SSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRM 183

Query: 187 IFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAHGARA 246
           IFHT DV+  T+GL  Y+VVFLAALVG+ +E K + ++HL KHMA G+ LMLRSAH  RA
Sbjct: 184 IFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRA 243

Query: 247 FLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARK 282
           FLYP+VD  + +  GF++L+++HPTD+V+NSVVIARK
Sbjct: 244 FLYPIVD--SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

BLAST of CsaV3_2G007090 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 315.5 bits (807), Expect = 5.2e-86
Identity = 158/273 (57.88%), Postives = 206/273 (75.46%), Query Frame = 0

Query: 10  LLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASL-SQPLRAMR 69
           L+++++  LYNQIS+L SLKP KNVDTLF QLV TC    P     D+  +  + ++ MR
Sbjct: 7   LVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCL---PTDTNIDVTEIHDEKVKDMR 66

Query: 70  AHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSHSRRI 129
           +HLI+LC +AE  LE HFS++L S   +P+++L IFPYY+NYLKL  LEFD+L  H+  +
Sbjct: 67  SHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHV 126

Query: 130 PDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFHT 189
           P KVAF+GSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  D DLS+RMIFHT
Sbjct: 127 PTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHT 186

Query: 190 KDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLYP 249
            DV+   +GL  Y+VVFLAALVG+ +E K + ++HL KHMA G+ +MLRSAHG RAFLYP
Sbjct: 187 TDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYP 246

Query: 250 VVDICTVEASGFEILSVFHPTDEVINSVVIARK 282
           +VD C  +  GFE+L+++HP+D+V+NSVVIARK
Sbjct: 247 IVDSC--DLKGFEVLTIYHPSDDVVNSVVIARK 274

BLAST of CsaV3_2G007090 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 315.1 bits (806), Expect = 6.8e-86
Identity = 160/276 (57.97%), Postives = 213/276 (77.17%), Query Frame = 0

Query: 7   QEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASLSQPLRA 66
           Q+  L++ +C LY +IS L SLKP ++V+ LF QLV TC    PP    D+  +   ++ 
Sbjct: 4   QDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCI---PPNPNIDVTKMCDRVQE 63

Query: 67  MRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSH-S 126
           +R +LI++C  AE  LE HFSS+L S   +P+ +L+IFPYY+NYLKL  LEFD+L  + +
Sbjct: 64  IRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLN 123

Query: 127 RRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMI 186
             +P  VAF+GSGPLPL+SIVLAS HLK TIFHNFDIDP+AN++AS LV SDPD+SQRM 
Sbjct: 124 GFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMF 183

Query: 187 FHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAF 246
           FHT D+M+VT+ LK ++VVFLAALVG+ +EEK +V++HL KHMA G+ LMLRSAHG RAF
Sbjct: 184 FHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAF 243

Query: 247 LYPVVDICTVEASGFEILSVFHPTDEVINSVVIARK 282
           LYP+V+ C ++  GFE+LS++HPTD+VINSVVI++K
Sbjct: 244 LYPIVEPCDLQ--GFEVLSIYHPTDDVINSVVISKK 274

BLAST of CsaV3_2G007090 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 55.1 bits (131), Expect = 1.3e-07
Identity = 29/56 (51.79%), Postives = 39/56 (69.64%), Query Frame = 0

Query: 228 MAAGSYLMLRSAHGARAFLYPVVDICTVEASGFEILSVFHP--TDEVINSVVIARK 282
           M  G+ LMLRSA G R+FLY  VD C  +  GFE+L ++HP  +D  +NSV++ARK
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPC--DLKGFEVLEIYHPSMSDGFVNSVMVARK 54

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004144750.16.8e-181100.00probable nicotianamine synthase 4 [Cucumis sativus] >KGN61000.1 hypothetical pro... [more]
KAA0048069.15.6e-16792.60putative nicotianamine synthase 4 isoform X2 [Cucumis melo var. makuwa] >TYJ9646... [more]
XP_038890564.16.2e-15084.68probable nicotianamine synthase 4 [Benincasa hispida][more]
XP_008453482.14.9e-14794.85PREDICTED: probable nicotianamine synthase 4 isoform X2 [Cucumis melo][more]
XP_008453473.11.6e-14594.20PREDICTED: probable nicotianamine synthase 4 isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9C7X56.4e-8953.68Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S... [more]
Q9XGI77.8e-8761.62Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1[more]
Q9FF794.3e-8558.12Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1[more]
Q9FKT97.3e-8557.88Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1[more]
O804839.5e-8557.97Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LGI43.3e-181100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034530 PE=3 SV=1[more]
A0A5D3BBH02.7e-16792.60Putative nicotianamine synthase 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3BXI82.4e-14794.85probable nicotianamine synthase 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A1S3BWE47.7e-14694.20probable nicotianamine synthase 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A6J1G3L49.5e-12877.64nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111450477 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT1G56430.14.5e-9053.68nicotianamine synthase 4 [more]
AT5G04950.13.0e-8658.12nicotianamine synthase 1 [more]
AT5G56080.15.2e-8657.88nicotianamine synthase 2 [more]
AT1G09240.16.8e-8657.97nicotianamine synthase 3 [more]
AT4G26483.11.3e-0751.79nicotianamine synthases [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004298Nicotianamine synthasePFAMPF03059NAScoord: 5..283
e-value: 3.6E-112
score: 374.2
IPR004298Nicotianamine synthasePANTHERPTHR32266NICOTIANAMINE SYNTHASE 3coord: 1..285
IPR004298Nicotianamine synthasePROSITEPS51142NAScoord: 3..286
score: 52.765114
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 12..283
e-value: 2.8E-60
score: 205.7

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_2G007090.1CsaV3_2G007090.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030418 nicotianamine biosynthetic process
molecular_function GO:0030410 nicotianamine synthase activity