CsaV3_2G004820 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_2G004820 vs. NCBI nr
Match: XP_011648795.1 (desmoplakin [Cucumis sativus] >KGN60890.2 hypothetical protein Csa_023473 [Cucumis sativus])

HSP 1 Score: 1055.8 bits (2729), Expect = 1.3e-304
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 0

Query: 1   MAKKKPTRSAKELKQTPNNQEETSDSEQPKSAMDDDSKLQSLKSLNERLLKEMVEKRVEV 60
           MAKKKPTRSAKELKQTPNNQEETSDSEQPKSAMDDDSKLQSLKSLNERLLKEMVEKRVEV
Sbjct: 1   MAKKKPTRSAKELKQTPNNQEETSDSEQPKSAMDDDSKLQSLKSLNERLLKEMVEKRVEV 60

Query: 61  GDLVQSKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRIQEMGGGI 120
           GDLVQSKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRIQEMGGGI
Sbjct: 61  GDLVQSKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRIQEMGGGI 120

Query: 121 CGLLESERVKGLEIRNLKAEITGLVSEVGEEREKWRGVCCERDEIKVEFDGLLKETGDLR 180
           CGLLESERVKGLEIRNLKAEITGLVSEVGEEREKWRGVCCERDEIKVEFDGLLKETGDLR
Sbjct: 121 CGLLESERVKGLEIRNLKAEITGLVSEVGEEREKWRGVCCERDEIKVEFDGLLKETGDLR 180

Query: 181 GKVVEMERNERRALEEIDDLKGKCKKLLSEKKECEILNANLTKDNELIKKLLEESGRVIE 240
           GKVVEMERNERRALEEIDDLKGKCKKLLSEKKECEILNANLTKDNELIKKLLEESGRVIE
Sbjct: 181 GKVVEMERNERRALEEIDDLKGKCKKLLSEKKECEILNANLTKDNELIKKLLEESGRVIE 240

Query: 241 DLERKVDVKMKEKGEIEKEKNGLEMEVEKLEKEVTQLKQSTFCLKQEKEENGMRIYELQM 300
           DLERKVDVKMKEKGEIEKEKNGLEMEVEKLEKEVTQLKQSTFCLKQEKEENGMRIYELQM
Sbjct: 241 DLERKVDVKMKEKGEIEKEKNGLEMEVEKLEKEVTQLKQSTFCLKQEKEENGMRIYELQM 300

Query: 301 RNEEALVKESGMLMECDLLVKELQKKEKAMEKAKTEAQNIIGDLQKESSKLKEAIASLTK 360
           RNEEALVKESGMLMECDLLVKELQKKEKAMEKAKTEAQNIIGDLQKESSKLKEAIASLTK
Sbjct: 301 RNEEALVKESGMLMECDLLVKELQKKEKAMEKAKTEAQNIIGDLQKESSKLKEAIASLTK 360

Query: 361 MSDVGKARNEELINQIGRLRDTLDEVSFERDDARKRFGDEKEKVEKLSLLLKDKERRIEE 420
           MSDVGKARNEELINQIGRLRDTLDEVSFERDDARKRFGDEKEKVEKLSLLLKDKERRIEE
Sbjct: 361 MSDVGKARNEELINQIGRLRDTLDEVSFERDDARKRFGDEKEKVEKLSLLLKDKERRIEE 420

Query: 421 AIKEVEKAKIAQEEESLNVKKEMERRINALIGERELMEKNLLAAKSRIDELKAKVNSAVC 480
           AIKEVEKAKIAQEEESLNVKKEMERRINALIGERELMEKNLLAAKSRIDELKAKVNSAVC
Sbjct: 421 AIKEVEKAKIAQEEESLNVKKEMERRINALIGERELMEKNLLAAKSRIDELKAKVNSAVC 480

Query: 481 NSEKALSLLKKTRLTVCDGYGKREVEEVSSDEHKVGKEMQPFVEHLDAIKTSFTNKEKAV 540
           NSEKALSLLKKTRLTVCDGYGKREVEEVSSDEHKVGKEMQPFVEHLDAIKTSFTNKEKAV
Sbjct: 481 NSEKALSLLKKTRLTVCDGYGKREVEEVSSDEHKVGKEMQPFVEHLDAIKTSFTNKEKAV 540

Query: 541 EEMTRVLETERMEQQKKKSFFTVVTAATTILAAVSALYVSKGR 584
           EEMTRVLETERMEQQKKKSFFTVVTAATTILAAVSALYVSKGR
Sbjct: 541 EEMTRVLETERMEQQKKKSFFTVVTAATTILAAVSALYVSKGR 583

BLAST of CsaV3_2G004820 vs. NCBI nr
Match: XP_008455286.1 (PREDICTED: cingulin [Cucumis melo])

HSP 1 Score: 948.3 bits (2450), Expect = 3.0e-272
Identity = 538/621 (86.63%), Postives = 556/621 (89.53%), Query Frame = 0

Query: 1   MAKKKPTRSAKELKQTPNNQEETSDSEQPKSAMDDDSKLQSLKSLNERLLKEMVEKRVEV 60
           MAKKKPTRSAKE KQ PNNQEETSDSEQP+SAMDDDSKLQSLKSLNERLLKEMVEKRV V
Sbjct: 1   MAKKKPTRSAKEPKQIPNNQEETSDSEQPRSAMDDDSKLQSLKSLNERLLKEMVEKRVVV 60

Query: 61  GDLVQSKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRIQEMGGGI 120
           GDLVQ+KEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRI+EM GGI
Sbjct: 61  GDLVQTKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRIEEMSGGI 120

Query: 121 CGLLESERVKGLEIRNLKAEITGLVSEVGEEREKWRGVCCERDEIKVEFDGLLKETGDLR 180
            GLLESERVKGLEIRNLKAEI GLV EV EEREKWRGVCCERDEIKVEFDGLLKETGDLR
Sbjct: 121 FGLLESERVKGLEIRNLKAEINGLVLEVEEEREKWRGVCCERDEIKVEFDGLLKETGDLR 180

Query: 181 GKVVEMERNERRALEEIDDLKGKCKKLLSEKKECEILNANLTKDNELIKKLLEESGRVIE 240
           GKVVEMERNERR LEEIDDLKGKCKKLLSEKKE EILN NLTKDNELIKKLLEESGRVIE
Sbjct: 181 GKVVEMERNERRTLEEIDDLKGKCKKLLSEKKEREILNGNLTKDNELIKKLLEESGRVIE 240

Query: 241 DLERKVDVKMKEKGEIEKEKNGLEMEVEKLEKEVTQLKQSTFCLKQEKEENGMRIYELQM 300
           DLERKVDVKMKEKGEIEKEKNGL+MEVEKLEKEV QLK+STFC KQEKEENG RI ELQM
Sbjct: 241 DLERKVDVKMKEKGEIEKEKNGLKMEVEKLEKEVAQLKESTFCFKQEKEENGKRISELQM 300

Query: 301 RNEEALVKESGMLMECDLLVKELQKKEKA------------------------------- 360
           R EEALVKESGMLME D+LVKELQKKE A                               
Sbjct: 301 RIEEALVKESGMLMEFDVLVKELQKKENAMEMLTQQRDSLDVNLNLIQEEAKSLQRTLEI 360

Query: 361 -------MEKAKTEAQNIIGDLQKESSKLKEAIASLTKMSDVGKARNEELINQIGRLRDT 420
                  ME+AKTEAQNIIGDLQKESSKLKEAIASLTKMSDVGKARNEELI QIGRLRD 
Sbjct: 361 LTHDKAEMEEAKTEAQNIIGDLQKESSKLKEAIASLTKMSDVGKARNEELIIQIGRLRDA 420

Query: 421 LDEVSFERDDARKRFGDEKEKVEKLSLLLKDKERRIEEAIKEVEKAKIAQEEESLNVKKE 480
           LDEVSFERDDARKRFGDEKE  EKL LLLKDKERRIEEA+KE++KAKIAQEE+SLNVKKE
Sbjct: 421 LDEVSFERDDARKRFGDEKENAEKLRLLLKDKERRIEEAVKELDKAKIAQEEDSLNVKKE 480

Query: 481 MERRINALIGERELMEKNLLAAKSRIDELKAKVNSAVCNSEKALSLLKKTRLTVCDGYGK 540
           MERR+ ALIGER+LMEKNLLAAK RIDELKAKVNSAVCNSEKAL+LLKKTRLTVCDGYGK
Sbjct: 481 MERRLVALIGERDLMEKNLLAAKIRIDELKAKVNSAVCNSEKALALLKKTRLTVCDGYGK 540

Query: 541 REVEEVSSDEHKVGKEMQPFVEHLDAIKTSFTNKEKAVEEMTRVLETERMEQQKKKSFFT 584
            EVEE SSDEHK+G+EMQPFVEHLDAIKTSFTNKEKAVEEMTRVLETER+EQQKKKSFFT
Sbjct: 541 GEVEEASSDEHKIGEEMQPFVEHLDAIKTSFTNKEKAVEEMTRVLETERVEQQKKKSFFT 600

BLAST of CsaV3_2G004820 vs. NCBI nr
Match: KAA0063670.1 (cingulin [Cucumis melo var. makuwa] >TYK18356.1 cingulin [Cucumis melo var. makuwa])

HSP 1 Score: 945.3 bits (2442), Expect = 2.5e-271
Identity = 536/621 (86.31%), Postives = 556/621 (89.53%), Query Frame = 0

Query: 1   MAKKKPTRSAKELKQTPNNQEETSDSEQPKSAMDDDSKLQSLKSLNERLLKEMVEKRVEV 60
           MAKKKPTRSA+E KQ PNNQEETSDSEQP+SAMDDDSKLQSLKSLNERLLKEMVEKRV V
Sbjct: 1   MAKKKPTRSAEEPKQIPNNQEETSDSEQPRSAMDDDSKLQSLKSLNERLLKEMVEKRVVV 60

Query: 61  GDLVQSKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRIQEMGGGI 120
           GDLVQ+KEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRI+EM GGI
Sbjct: 61  GDLVQTKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRIEEMSGGI 120

Query: 121 CGLLESERVKGLEIRNLKAEITGLVSEVGEEREKWRGVCCERDEIKVEFDGLLKETGDLR 180
            GLLESERVKGLEIRNLKAEI GLV EV EEREKWRGVCCERDEIKVEFDGLLKETGDLR
Sbjct: 121 FGLLESERVKGLEIRNLKAEINGLVLEVEEEREKWRGVCCERDEIKVEFDGLLKETGDLR 180

Query: 181 GKVVEMERNERRALEEIDDLKGKCKKLLSEKKECEILNANLTKDNELIKKLLEESGRVIE 240
           GKVVEMERNERR LEEIDDLKGKCKKLLSEKKE EILN NLTKDNELIKKLLEESGRVIE
Sbjct: 181 GKVVEMERNERRTLEEIDDLKGKCKKLLSEKKEREILNGNLTKDNELIKKLLEESGRVIE 240

Query: 241 DLERKVDVKMKEKGEIEKEKNGLEMEVEKLEKEVTQLKQSTFCLKQEKEENGMRIYELQM 300
           DLERKVDVKMKEKGEIEKEKNGL+MEVEKLEKEV QLK+STFC KQEKEENG RI ELQM
Sbjct: 241 DLERKVDVKMKEKGEIEKEKNGLKMEVEKLEKEVAQLKESTFCFKQEKEENGKRISELQM 300

Query: 301 RNEEALVKESGMLMECDLLVKELQKKEKA------------------------------- 360
           R EEALVKESGMLME D+LVKELQKKE A                               
Sbjct: 301 RIEEALVKESGMLMEFDVLVKELQKKENAMEMLTQQRDSLDVNLNLIQEEAKSLQRTLEI 360

Query: 361 -------MEKAKTEAQNIIGDLQKESSKLKEAIASLTKMSDVGKARNEELINQIGRLRDT 420
                  ME+AKTEAQNIIGDLQKESSKLKEAIASLTKMSDVGKARNEELI QIGRLRD 
Sbjct: 361 LTHDKAEMEEAKTEAQNIIGDLQKESSKLKEAIASLTKMSDVGKARNEELIIQIGRLRDA 420

Query: 421 LDEVSFERDDARKRFGDEKEKVEKLSLLLKDKERRIEEAIKEVEKAKIAQEEESLNVKKE 480
           LDEVSFERDDARKRFGDEKE  EKL LLLKDKERRIEEA+KE++KAKIAQEE+SLNVKKE
Sbjct: 421 LDEVSFERDDARKRFGDEKENAEKLRLLLKDKERRIEEAVKELDKAKIAQEEDSLNVKKE 480

Query: 481 MERRINALIGERELMEKNLLAAKSRIDELKAKVNSAVCNSEKALSLLKKTRLTVCDGYGK 540
           MERR+ ALIGER+LMEKNLLAAK RIDEL+AKVNSAVCNSEKAL+LLKKTRLTVCDGYGK
Sbjct: 481 MERRLVALIGERDLMEKNLLAAKIRIDELEAKVNSAVCNSEKALALLKKTRLTVCDGYGK 540

Query: 541 REVEEVSSDEHKVGKEMQPFVEHLDAIKTSFTNKEKAVEEMTRVLETERMEQQKKKSFFT 584
            EVEE SSDEHK+G+EMQPFVEHLDAIKTSFTNKEKAVEEMTRVLETER+EQQKKKSFFT
Sbjct: 541 GEVEEASSDEHKIGEEMQPFVEHLDAIKTSFTNKEKAVEEMTRVLETERVEQQKKKSFFT 600

BLAST of CsaV3_2G004820 vs. NCBI nr
Match: XP_038889361.1 (paramyosin-like [Benincasa hispida])

HSP 1 Score: 811.6 bits (2095), Expect = 4.4e-231
Identity = 469/621 (75.52%), Postives = 519/621 (83.57%), Query Frame = 0

Query: 1   MAKKKPTRSAKELKQTPNNQEETSDSEQPKSAMDDDSKLQSLKSLNERLLKEMVEKRVEV 60
           MAKKK TRSA E KQ P  QEE SD EQP SAMDDDSKLQSLKSLNERLLK++VEKRVEV
Sbjct: 1   MAKKKSTRSANEPKQMPIQQEENSDLEQPISAMDDDSKLQSLKSLNERLLKQVVEKRVEV 60

Query: 61  GDLVQSKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRIQEMGGGI 120
           GDLV SKEALELDLKRNV+EKEQVMGEL+EARDGVYGLELERNVVCVYLQSR++EMG G+
Sbjct: 61  GDLVHSKEALELDLKRNVDEKEQVMGELTEARDGVYGLELERNVVCVYLQSRMEEMGNGM 120

Query: 121 CGLLESERVKGLEIRNLKAEITGLVSEVGEEREKWRGVCCERDEIKVEFDGLLKETGDLR 180
           CGLLESERVKGLEIR LK+EI  L  EV EEREKWR VCCERD IKV+FD L KETGDL+
Sbjct: 121 CGLLESERVKGLEIRRLKSEINVLALEVEEEREKWRRVCCERDGIKVQFDDLFKETGDLK 180

Query: 181 GKVVEMERNERRALEEIDDLKGKCKKLLSEKKECEILNANLTKDNELIKKLLEESGRVIE 240
           GKVVEMERNE RALEEIDDLKGKCKKLL+EKKEC+I+N  L KDNELIKKLL+ESGRV+E
Sbjct: 181 GKVVEMERNETRALEEIDDLKGKCKKLLTEKKECDIMNGTLMKDNELIKKLLDESGRVVE 240

Query: 241 DLERKVDVKMKEKGEIEKEKNGLEMEVEKLEKEVTQLKQSTFCLKQEKEENGMRIYELQM 300
           DLERKVDVKMKEK EIEKEKNGL+ME+EKLE+EV +LK+STFC KQEKEENG ++ ELQM
Sbjct: 241 DLERKVDVKMKEKVEIEKEKNGLKMEIEKLEREVAELKESTFCFKQEKEENGKKLSELQM 300

Query: 301 RNEEALVKESGMLMECDLLVKELQKKEKA------------------------------- 360
           R EEA+ KESGMLME D+LVKELQKKEKA                               
Sbjct: 301 RIEEAVEKESGMLMESDVLVKELQKKEKAMEMLTQERDSLDLNFNLVQEEAKSLRRTIEI 360

Query: 361 -------MEKAKTEAQNIIGDLQKESSKLKEAIASLTKMSDVGKARNEELINQIGRLRDT 420
                  ME+AKTEA+NIIGDLQKESSKLKEAIASLTKM+DV KARNE+L+ +IGRLRD 
Sbjct: 361 LTRDKVEMEEAKTEAENIIGDLQKESSKLKEAIASLTKMTDVEKARNEDLLTEIGRLRDA 420

Query: 421 LDEVSFERDDARKRFGDEKEKVEKLSLLLKDKERRIEEAIKEVEKAKIAQEEESLNVKKE 480
           LDEVS ER+ ARK FGDEK+ VEKLSLLLKD+ER+ EEA+ E++KAKIAQ E+SLNVKKE
Sbjct: 421 LDEVSLEREGARKNFGDEKKNVEKLSLLLKDEERKFEEAMNELDKAKIAQ-EDSLNVKKE 480

Query: 481 MERRINALIGERELMEKNLLAAKSRIDELKAKVNSAVCNSEKALSLLKKTRLTVCDGYGK 540
           M RRI+ LI ER+ +EK+LL AKSRIDELK KV SAV NSEKAL+LLKKT L VCDGY K
Sbjct: 481 MGRRIDILIRERDSLEKSLLEAKSRIDELKDKVKSAVGNSEKALALLKKTCLAVCDGYEK 540

Query: 541 REVEEVSSDEHKVGKEMQPFVEHLDAIKTSFTNKEKAVEEMTRVLETERMEQQKKKSFFT 584
            EVEE SS  HK+ +E+QPFVEHLDAIKTSFTNKEK VEEM R LE ER E++KKKSFFT
Sbjct: 541 GEVEEASSG-HKLVEEIQPFVEHLDAIKTSFTNKEKMVEEMKRQLENERAEERKKKSFFT 600

BLAST of CsaV3_2G004820 vs. NCBI nr
Match: XP_022150832.1 (myosin-2 heavy chain, non muscle-like [Momordica charantia])

HSP 1 Score: 631.7 bits (1628), Expect = 6.2e-177
Identity = 389/627 (62.04%), Postives = 471/627 (75.12%), Query Frame = 0

Query: 1   MAKKKPTRSAKELKQTPNN-QEETSDSEQPKSAMDDD-SKLQSLKSLNERLLKEMVEKRV 60
           MAKKK TR AKE KQ  N  Q+E SD EQ ++AMDD   KLQSLKSLN+RL+KE  E+R+
Sbjct: 1   MAKKKATRVAKEPKQMQNQPQQEDSDPEQSRAAMDDSVLKLQSLKSLNDRLVKETHERRM 60

Query: 61  EVGDLVQSKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRIQEMGG 120
           EVG LV++K+ALE+DLKRNV+EK QVMGEL EA +G+YGL+LE+NVV V+LQS+++EMGG
Sbjct: 61  EVGALVKTKDALEIDLKRNVDEKNQVMGELGEACEGIYGLDLEKNVVSVFLQSQMEEMGG 120

Query: 121 GICGLL----ESERVKGLEIRNLKAEITGLVSEVGEEREKWRGVCCERDEIKVEFDGLLK 180
           GICGL+    ESER+K +EI  LKAE+  LV +V EEREKWR V  ERD +K+ FDGLL+
Sbjct: 121 GICGLMAEKRESERLKEMEIGLLKAEVNELVLKVEEEREKWRRVRSERDAMKIAFDGLLE 180

Query: 181 ETGDLRGKVVEMERNERRALEEIDDLKGKCKKLLSEKKECEILNANLTKDNELIKKLLEE 240
           ETGDLRGK   MERNER ALEEI  LKGKC+KL+ EK E E++N  L K+NE +KKLL+E
Sbjct: 181 ETGDLRGK---MERNERMALEEIAGLKGKCEKLMGEKMEIEVVNGTLLKENESVKKLLDE 240

Query: 241 SGRVIEDLERKVDVKMKEKGEIEKEKNGLEMEVEKLEKEVTQLKQSTFCLKQEKEENGMR 300
           S  VIEDLERK++ KMKEK EIE+EK+GL+ME+ KLEKEV QL +STF  KQEK+EN  R
Sbjct: 241 SAVVIEDLERKMEEKMKEKVEIEREKDGLQMEILKLEKEVVQLNESTFSFKQEKKENEQR 300

Query: 301 IYELQMRNEEALVKESGMLMECDLLVKELQKKEK-------------------------- 360
           I E++ R EEA+ KE+GMLME D LVK+LQKKEK                          
Sbjct: 301 ISEIEKRTEEAIEKENGMLMEIDALVKQLQKKEKDMEMLTQQRESLELNLNLVQEEVGNL 360

Query: 361 ------------AMEKAKTEAQNIIGDLQKESSKLKEAIASLTKMSDVGKARNEELINQI 420
                        ME+ K EA+NIIG+LQ+ESSKLKEAI SLT++ DV KARNEEL+++I
Sbjct: 361 RRTIEVITRDKVEMEEKKKEAENIIGELQRESSKLKEAITSLTELGDVEKARNEELLSEI 420

Query: 421 GRLRDTLDEVSFERDDARKRFGDEKEKVEKLSLLLKDKERRIEEAIKEVEKAKIAQEEES 480
            RLRD L EVSFERDDARK F DEK  VEKLSLLLKDKE R+ EA        +  +E+S
Sbjct: 421 SRLRDALVEVSFERDDARKSFSDEKGSVEKLSLLLKDKESRLVEA--------MISQEDS 480

Query: 481 LNVKKEMERRINALIGERELMEKNLLAAKSRIDELKAKVNSAVCNSEKALSLLKKTRLTV 540
           LN+KKEME+RI+ L+GER+ MEKNLL A+ RID+LKA+V SAV NSEKAL+LLKKT L V
Sbjct: 481 LNIKKEMEKRIDILVGERDSMEKNLLEAQRRIDDLKAEVKSAVANSEKALALLKKTCLAV 540

Query: 541 CDGYGKREVEEVSSDEHKVGKEMQPFVEHLDAIKTSFTNKEKAVEEMTRVLETERMEQQK 584
           CDGY ++ V E SS         +PFVEHL+AI+TSFTNKEK VEEM   LET R+E ++
Sbjct: 541 CDGY-EKGVGEASS---------EPFVEHLNAIRTSFTNKEKMVEEMKHRLETARVE-ER 600

BLAST of CsaV3_2G004820 vs. ExPASy Swiss-Prot
Match: P24733 (Myosin heavy chain, striated muscle OS=Argopecten irradians OX=31199 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 4.9e-07
Identity = 119/537 (22.16%), Postives = 243/537 (45.25%), Query Frame = 0

Query: 74   LKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRIQEMGGGICGLLESERVKGLE 133
            +K ++ + E++  EL E    V  LE ++N + + LQ+    MG       + ERV+ L 
Sbjct: 856  MKEDLAKTERIKKELEE--QNVTLLE-QKNDLFLQLQTLEDSMGD------QEERVEKLI 915

Query: 134  IRNLKAEITGLVSEVGEEREKWRGVCCERDEIKVEFD----GLLKETGDLRGKVVEMERN 193
            ++  KA+    + E+ E          + + IK + +     L K+ GDL   + + E++
Sbjct: 916  MQ--KADFESQIKELEERLLDEEDAAADLEGIKKKMEADNANLKKDIGDLENTLQKAEQD 975

Query: 194  ERRALEEIDDLKG-------------KCKKLLSE--KKECEILNANLTKDNELIKKLLEE 253
            +     +I  L+G             K KK L E  KK  + L A   K N L  KL  +
Sbjct: 976  KAHKDNQISTLQGEISQQDEHIGKLNKEKKALEEANKKTSDSLQAEEDKCNHL-NKLKAK 1035

Query: 254  SGRVIEDLERKVDVKMKEKGEIEKEKNGLEMEVEKLEKEVTQLKQSTFCLKQEKEENGMR 313
              + +++LE  ++ + K +G++EK K  +E +++  ++ V  L++    +K+E EEN  R
Sbjct: 1036 LEQALDELEDNLEREKKVRGDVEKAKRKVEQDLKSTQENVEDLER----VKRELEENVRR 1095

Query: 314  IYELQMRNEEALVKESGMLMECDLLVKELQKKEKAME---------KAKTEAQ--NIIGD 373
                       L  E  ++ +    +KELQ + + +E         +AK E Q   +  +
Sbjct: 1096 KEAEISSLNSKLEDEQNLVSQLQRKIKELQARIEELEEELEAERNARAKVEKQRAELNRE 1155

Query: 374  LQKESSKLKEAIASLTKMSDVGKARNEELINQIGRLRDTLDEVSFERD-----------D 433
            L++   +L EA  + +   ++ K R  EL+    ++R  L+E S + +           D
Sbjct: 1156 LEELGERLDEAGGATSAQIELNKKREAELL----KIRRDLEEASLQHEAQISALRKKHQD 1215

Query: 434  ARKRFGDEKEKVEKLSLLLKDKERRIEEAIKEVEKA---KIAQEEESLNVKKEMERR--- 493
            A     D+ ++++K+   L+  ++ ++  + ++E      +  +  S  V K+ E +   
Sbjct: 1216 AANEMADQVDQLQKVKSKLEKDKKDLKREMDDLESQMTHNMKNKGCSEKVMKQFESQMSD 1275

Query: 494  INALIGERELMEKNLLAAKSRIDELKAKVNSAVCNSEKALSLLKKTRLTVCDGY--GKRE 553
            +NA + + +     L + KSR+    + +   + ++E  +S+L K +  +       +R 
Sbjct: 1276 LNARLEDSQRSINELQSQKSRLQAENSDLTRQLEDAEHRVSVLSKEKSQLSSQLEDARRS 1335

Query: 554  VEEVSSDEHKVGKEMQPFVEHLDAIKTSFTNKEKAVEEMTRVLETERMEQQKKKSFF 562
            +EE +    K+  E++     +DAI+     ++++  ++ R L     E Q+ +S F
Sbjct: 1336 LEEETRARSKLQNEVRNMHADMDAIREQLEEEQESKSDVQRQLSKANNEIQQWRSKF 1372

BLAST of CsaV3_2G004820 vs. ExPASy Swiss-Prot
Match: O29230 (DNA double-strand break repair Rad50 ATPase OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=rad50 PE=3 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 1.2e-05
Identity = 101/457 (22.10%), Postives = 210/457 (45.95%), Query Frame = 0

Query: 126 SERVKGLEI--RNLKAEITGLVSEVGE-EREKWRGVCCERDEIKVEFDGLLKETGDLRGK 185
           SE +K +E     L  E+  L S + E E  K R      + ++ +   +L+E   L  K
Sbjct: 200 SEEIKSIESLREKLSEEVRNLESRLKELEEHKSR-----LESLRKQESSVLQEVRGLEEK 259

Query: 186 VVEMERNERRALEEIDDLKGKCKKLLSEKKECEILNANLTKDNELIKKLLEESGRVIEDL 245
           + E+E+  +  +E I+DL+ K K++   K + E  +        +++KLL E  + + D+
Sbjct: 260 LRELEKQLKEVVERIEDLEKKAKEVKELKPKAERYS--------ILEKLLSEINQALRDV 319

Query: 246 ERKVDVKMKEKGEIEKEKNGLEMEVEKLEKEVTQLKQSTFCLKQ-----EKEENGMRIYE 305
           E       K +G++ +E  G++ +++K E++ ++L++ T  +++     E+ E   R+ E
Sbjct: 320 E-------KREGDLTREAAGIQAQLKKAEEDNSKLEEITKRIEELERELERFEKSHRLLE 379

Query: 306 LQMRNEEALVKESGMLMECDLLVKELQKKEKAMEKAKTEAQNIIGDLQK----------E 365
                 + +      L E +L   +++K    + KAK E + I   L+K           
Sbjct: 380 TLKPKMDRMQGIKAKLEEKNLTPDKVEKMYDLLSKAKEEEKEITEKLKKLIAKKSSLKTR 439

Query: 366 SSKLKEAIASLTKMS--------DVGKARNEELINQIGRLRDTLDEVSFERDDARKRFGD 425
            ++LK+A+  L            ++ +   + ++ +  R    + E   + D+  K+  +
Sbjct: 440 GAQLKKAVEELKSAERTCPVCGRELDEEHRKNIMAEYTREMKRIAEELAKADEIEKKLKE 499

Query: 426 EKEKVEKLSLLLKD--KERRIEEAIKEVEKAKIAQEEESLNVKKEMERRINALIGERELM 485
             EKVEK     +   K R++ + +K +E    + + E L+ + E  R++   +      
Sbjct: 500 RLEKVEKALEKQETVLKYRQMVDELKALENELSSHDAEKLSAESEEYRKVKERLDGLRGQ 559

Query: 486 EKNLLAAKSRIDELKAKVNSAVCNSEKALSLLKKTRLTVCDGYGKREVEEVSSDEHKVGK 545
           +K LL++ SRI ELK    S++   E+AL  ++  R  +     +   E +   E +V +
Sbjct: 560 QKILLSSASRIKELK----SSLREIEEALKNVESERGELHRKIREEGFESLEELEREV-Q 619

Query: 546 EMQPF----VEHLDA---IKTSFTNKEKAVEEMTRVL 548
            ++PF    +E  DA   +++    +EK  +E++  +
Sbjct: 620 SLRPFYNKWLELKDAESRLESELKRREKLEDEISEAI 631

BLAST of CsaV3_2G004820 vs. ExPASy Swiss-Prot
Match: O67124 (Probable DNA double-strand break repair Rad50 ATPase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=rad50 PE=3 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 6.6e-04
Identity = 103/441 (23.36%), Postives = 198/441 (44.90%), Query Frame = 0

Query: 168 EFDGLLKETGDLRGKVV------EMERNERRALEEIDDLKGKCKKLLSEKKECEILNANL 227
           EFD  LKE+ + +  ++      E+E+  + A E   +L+GK + L   KKE E+L    
Sbjct: 142 EFDRFLKESSERKKILINLLGLEELEKVRQLASETFKNLEGKREAL---KKEYELLKDYT 201

Query: 228 TKDNELIKKL---LEESGRVIEDLERKVDVKMK---EKGEIEKEKNGLE---MEVEKLEK 287
               E+++K    LEE  + +++ E K+  ++K   EK  +E+E + +     E+E LEK
Sbjct: 202 PTKKEVLEKTLKNLEEELKELKETEEKLRQELKKAEEKDSLERELSQVVTKLKELENLEK 261

Query: 288 EVTQLKQSTFCLKQEKE--ENGMRIYELQMRNEEALVKESGMLMECDLLVKELQKKEKAM 347
           EV +L++     ++         RI E+  +  E  V+++ +  E  +L  EL   ++ +
Sbjct: 262 EVEKLREKLEFSRKVAPYVPIAKRIEEIDKKLTELKVRKNKLTKELAVLKDELSFAQEEL 321

Query: 348 EKAKTEAQNIIGDLQKESSKLKEAIASLTKMSDVGKARNEELINQIGRLRDTLDEVSFER 407
            + + E +      ++E  + KE    L K+ ++     +E++ ++ +L  +L E   E 
Sbjct: 322 NRIEAEKEK----FKEEKEREKELEHRLKKLQEI-----KEILKELSQLSSSLKEKEREY 381

Query: 408 DDARKRFGDEKEKVEKLSLLLKDKERRIEEAIKEVEKAKIAQEEESLNVKKEMERRINAL 467
           + A++ F D  E+VEK   L+ + E ++E+ IKE+      +E  SL +K+ +   +   
Sbjct: 382 EQAKQEFEDLSERVEKGKKLVAETEEKLEK-IKELFS---EEEYTSLKMKERLLVELQRK 441

Query: 468 IGERELMEKNLLAAKSRIDELKAKVNSAVCNSEKALSLLKKTR----------------- 527
           + E +  E  L     +  E K KV+  V N  K L    K R                 
Sbjct: 442 LKELKEKEGQLENLTQKYKE-KKKVHEKVLNELKELERELKERELHYHAHMVASYLSPGD 501

Query: 528 -LTVCDG-YGKREVEEVSS---------------DEHKVGKEMQPFVEHLDAIKTSFTNK 558
              VC G Y  + +E V +               +E ++   ++ + + ++++K      
Sbjct: 502 TCPVCGGIYRGKALENVDAEGISELKHAKELKEKEEREIDTTLKLYAQKINSLKEEMEKL 561

BLAST of CsaV3_2G004820 vs. ExPASy TrEMBL
Match: A0A1S3C0Q0 (cingulin OS=Cucumis melo OX=3656 GN=LOC103495485 PE=4 SV=1)

HSP 1 Score: 948.3 bits (2450), Expect = 1.5e-272
Identity = 538/621 (86.63%), Postives = 556/621 (89.53%), Query Frame = 0

Query: 1   MAKKKPTRSAKELKQTPNNQEETSDSEQPKSAMDDDSKLQSLKSLNERLLKEMVEKRVEV 60
           MAKKKPTRSAKE KQ PNNQEETSDSEQP+SAMDDDSKLQSLKSLNERLLKEMVEKRV V
Sbjct: 1   MAKKKPTRSAKEPKQIPNNQEETSDSEQPRSAMDDDSKLQSLKSLNERLLKEMVEKRVVV 60

Query: 61  GDLVQSKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRIQEMGGGI 120
           GDLVQ+KEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRI+EM GGI
Sbjct: 61  GDLVQTKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRIEEMSGGI 120

Query: 121 CGLLESERVKGLEIRNLKAEITGLVSEVGEEREKWRGVCCERDEIKVEFDGLLKETGDLR 180
            GLLESERVKGLEIRNLKAEI GLV EV EEREKWRGVCCERDEIKVEFDGLLKETGDLR
Sbjct: 121 FGLLESERVKGLEIRNLKAEINGLVLEVEEEREKWRGVCCERDEIKVEFDGLLKETGDLR 180

Query: 181 GKVVEMERNERRALEEIDDLKGKCKKLLSEKKECEILNANLTKDNELIKKLLEESGRVIE 240
           GKVVEMERNERR LEEIDDLKGKCKKLLSEKKE EILN NLTKDNELIKKLLEESGRVIE
Sbjct: 181 GKVVEMERNERRTLEEIDDLKGKCKKLLSEKKEREILNGNLTKDNELIKKLLEESGRVIE 240

Query: 241 DLERKVDVKMKEKGEIEKEKNGLEMEVEKLEKEVTQLKQSTFCLKQEKEENGMRIYELQM 300
           DLERKVDVKMKEKGEIEKEKNGL+MEVEKLEKEV QLK+STFC KQEKEENG RI ELQM
Sbjct: 241 DLERKVDVKMKEKGEIEKEKNGLKMEVEKLEKEVAQLKESTFCFKQEKEENGKRISELQM 300

Query: 301 RNEEALVKESGMLMECDLLVKELQKKEKA------------------------------- 360
           R EEALVKESGMLME D+LVKELQKKE A                               
Sbjct: 301 RIEEALVKESGMLMEFDVLVKELQKKENAMEMLTQQRDSLDVNLNLIQEEAKSLQRTLEI 360

Query: 361 -------MEKAKTEAQNIIGDLQKESSKLKEAIASLTKMSDVGKARNEELINQIGRLRDT 420
                  ME+AKTEAQNIIGDLQKESSKLKEAIASLTKMSDVGKARNEELI QIGRLRD 
Sbjct: 361 LTHDKAEMEEAKTEAQNIIGDLQKESSKLKEAIASLTKMSDVGKARNEELIIQIGRLRDA 420

Query: 421 LDEVSFERDDARKRFGDEKEKVEKLSLLLKDKERRIEEAIKEVEKAKIAQEEESLNVKKE 480
           LDEVSFERDDARKRFGDEKE  EKL LLLKDKERRIEEA+KE++KAKIAQEE+SLNVKKE
Sbjct: 421 LDEVSFERDDARKRFGDEKENAEKLRLLLKDKERRIEEAVKELDKAKIAQEEDSLNVKKE 480

Query: 481 MERRINALIGERELMEKNLLAAKSRIDELKAKVNSAVCNSEKALSLLKKTRLTVCDGYGK 540
           MERR+ ALIGER+LMEKNLLAAK RIDELKAKVNSAVCNSEKAL+LLKKTRLTVCDGYGK
Sbjct: 481 MERRLVALIGERDLMEKNLLAAKIRIDELKAKVNSAVCNSEKALALLKKTRLTVCDGYGK 540

Query: 541 REVEEVSSDEHKVGKEMQPFVEHLDAIKTSFTNKEKAVEEMTRVLETERMEQQKKKSFFT 584
            EVEE SSDEHK+G+EMQPFVEHLDAIKTSFTNKEKAVEEMTRVLETER+EQQKKKSFFT
Sbjct: 541 GEVEEASSDEHKIGEEMQPFVEHLDAIKTSFTNKEKAVEEMTRVLETERVEQQKKKSFFT 600

BLAST of CsaV3_2G004820 vs. ExPASy TrEMBL
Match: A0A5D3D489 (Cingulin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00730 PE=4 SV=1)

HSP 1 Score: 945.3 bits (2442), Expect = 1.2e-271
Identity = 536/621 (86.31%), Postives = 556/621 (89.53%), Query Frame = 0

Query: 1   MAKKKPTRSAKELKQTPNNQEETSDSEQPKSAMDDDSKLQSLKSLNERLLKEMVEKRVEV 60
           MAKKKPTRSA+E KQ PNNQEETSDSEQP+SAMDDDSKLQSLKSLNERLLKEMVEKRV V
Sbjct: 1   MAKKKPTRSAEEPKQIPNNQEETSDSEQPRSAMDDDSKLQSLKSLNERLLKEMVEKRVVV 60

Query: 61  GDLVQSKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRIQEMGGGI 120
           GDLVQ+KEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRI+EM GGI
Sbjct: 61  GDLVQTKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRIEEMSGGI 120

Query: 121 CGLLESERVKGLEIRNLKAEITGLVSEVGEEREKWRGVCCERDEIKVEFDGLLKETGDLR 180
            GLLESERVKGLEIRNLKAEI GLV EV EEREKWRGVCCERDEIKVEFDGLLKETGDLR
Sbjct: 121 FGLLESERVKGLEIRNLKAEINGLVLEVEEEREKWRGVCCERDEIKVEFDGLLKETGDLR 180

Query: 181 GKVVEMERNERRALEEIDDLKGKCKKLLSEKKECEILNANLTKDNELIKKLLEESGRVIE 240
           GKVVEMERNERR LEEIDDLKGKCKKLLSEKKE EILN NLTKDNELIKKLLEESGRVIE
Sbjct: 181 GKVVEMERNERRTLEEIDDLKGKCKKLLSEKKEREILNGNLTKDNELIKKLLEESGRVIE 240

Query: 241 DLERKVDVKMKEKGEIEKEKNGLEMEVEKLEKEVTQLKQSTFCLKQEKEENGMRIYELQM 300
           DLERKVDVKMKEKGEIEKEKNGL+MEVEKLEKEV QLK+STFC KQEKEENG RI ELQM
Sbjct: 241 DLERKVDVKMKEKGEIEKEKNGLKMEVEKLEKEVAQLKESTFCFKQEKEENGKRISELQM 300

Query: 301 RNEEALVKESGMLMECDLLVKELQKKEKA------------------------------- 360
           R EEALVKESGMLME D+LVKELQKKE A                               
Sbjct: 301 RIEEALVKESGMLMEFDVLVKELQKKENAMEMLTQQRDSLDVNLNLIQEEAKSLQRTLEI 360

Query: 361 -------MEKAKTEAQNIIGDLQKESSKLKEAIASLTKMSDVGKARNEELINQIGRLRDT 420
                  ME+AKTEAQNIIGDLQKESSKLKEAIASLTKMSDVGKARNEELI QIGRLRD 
Sbjct: 361 LTHDKAEMEEAKTEAQNIIGDLQKESSKLKEAIASLTKMSDVGKARNEELIIQIGRLRDA 420

Query: 421 LDEVSFERDDARKRFGDEKEKVEKLSLLLKDKERRIEEAIKEVEKAKIAQEEESLNVKKE 480
           LDEVSFERDDARKRFGDEKE  EKL LLLKDKERRIEEA+KE++KAKIAQEE+SLNVKKE
Sbjct: 421 LDEVSFERDDARKRFGDEKENAEKLRLLLKDKERRIEEAVKELDKAKIAQEEDSLNVKKE 480

Query: 481 MERRINALIGERELMEKNLLAAKSRIDELKAKVNSAVCNSEKALSLLKKTRLTVCDGYGK 540
           MERR+ ALIGER+LMEKNLLAAK RIDEL+AKVNSAVCNSEKAL+LLKKTRLTVCDGYGK
Sbjct: 481 MERRLVALIGERDLMEKNLLAAKIRIDELEAKVNSAVCNSEKALALLKKTRLTVCDGYGK 540

Query: 541 REVEEVSSDEHKVGKEMQPFVEHLDAIKTSFTNKEKAVEEMTRVLETERMEQQKKKSFFT 584
            EVEE SSDEHK+G+EMQPFVEHLDAIKTSFTNKEKAVEEMTRVLETER+EQQKKKSFFT
Sbjct: 541 GEVEEASSDEHKIGEEMQPFVEHLDAIKTSFTNKEKAVEEMTRVLETERVEQQKKKSFFT 600

BLAST of CsaV3_2G004820 vs. ExPASy TrEMBL
Match: A0A0A0LII4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G015350 PE=4 SV=1)

HSP 1 Score: 892.9 bits (2306), Expect = 7.2e-256
Identity = 516/583 (88.51%), Postives = 516/583 (88.51%), Query Frame = 0

Query: 1   MAKKKPTRSAKELKQTPNNQEETSDSEQPKSAMDDDSKLQSLKSLNERLLKEMVEKRVEV 60
           MAKKKPTRSAKELKQTPNNQEETSDSEQPKSAMDDDSKLQSLKSLNERLLKEMVEKRVEV
Sbjct: 1   MAKKKPTRSAKELKQTPNNQEETSDSEQPKSAMDDDSKLQSLKSLNERLLKEMVEKRVEV 60

Query: 61  GDLVQSKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRIQEMGGGI 120
           GDLVQSKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRIQEMGGGI
Sbjct: 61  GDLVQSKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRIQEMGGGI 120

Query: 121 CGLLESERVKGLEIRNLKAEITGLVSEVGEEREKWRGVCCERDEIKVEFDGLLKETGDLR 180
           CGLLESERVKGLEIRNLKAEITGLVSE                                 
Sbjct: 121 CGLLESERVKGLEIRNLKAEITGLVSE--------------------------------- 180

Query: 181 GKVVEMERNERRALEEIDDLKGKCKKLLSEKKECEILNANLTKDNELIKKLLEESGRVIE 240
             VVEMERNERRALEEIDDLKGKCKKLLSEKKECEILNANLTKDNELIKKLLEESGRVIE
Sbjct: 181 --VVEMERNERRALEEIDDLKGKCKKLLSEKKECEILNANLTKDNELIKKLLEESGRVIE 240

Query: 241 DLERKVDVKMKEKGEIEKEKNGLEMEVEKLEKEVTQLKQSTFCLKQEKEENGMRIYELQM 300
           DLERKVDVKMKEKGEIEKEKNGLEMEVEKLEKEVTQLKQSTFCLKQEKEENGMRIYELQM
Sbjct: 241 DLERKVDVKMKEKGEIEKEKNGLEMEVEKLEKEVTQLKQSTFCLKQEKEENGMRIYELQM 300

Query: 301 RNEEALVKESGMLMECDLLVKELQKKEKAMEKAKTEAQNIIGDLQKESSKLKEAIASLTK 360
           RNEEAL                                NIIGDLQKESSKLKEAIASLTK
Sbjct: 301 RNEEAL--------------------------------NIIGDLQKESSKLKEAIASLTK 360

Query: 361 MSDVGKARNEELINQIGRLRDTLDEVSFERDDARKRFGDEKEKVEKLSLLLKDKERRIEE 420
           MSDVGKARNEELINQIGRLRDTLDEVSFERDDARKRFGDEKEKVEKLSLLLKDKERRIEE
Sbjct: 361 MSDVGKARNEELINQIGRLRDTLDEVSFERDDARKRFGDEKEKVEKLSLLLKDKERRIEE 420

Query: 421 AIKEVEKAKIAQEEESLNVKKEMERRINALIGERELMEKNLLAAKSRIDELKAKVNSAVC 480
           AIKEVEKAKIAQEEESLNVKKEMERRINALIGERELMEKNLLAAKSRIDELKAKVNSAVC
Sbjct: 421 AIKEVEKAKIAQEEESLNVKKEMERRINALIGERELMEKNLLAAKSRIDELKAKVNSAVC 480

Query: 481 NSEKALSLLKKTRLTVCDGYGKREVEEVSSDEHKVGKEMQPFVEHLDAIKTSFTNKEKAV 540
           NSEKALSLLKKTRLTVCDGYGKREVEEVSSDEHKVGKEMQPFVEHLDAIKTSFTNKEKAV
Sbjct: 481 NSEKALSLLKKTRLTVCDGYGKREVEEVSSDEHKVGKEMQPFVEHLDAIKTSFTNKEKAV 516

Query: 541 EEMTRVLETERMEQQKKKSFFTVVTAATTILAAVSALYVSKGR 584
           EEMTRVLETERMEQQKKKSFFTVVTAATTILAAVSALYVSKGR
Sbjct: 541 EEMTRVLETERMEQQKKKSFFTVVTAATTILAAVSALYVSKGR 516

BLAST of CsaV3_2G004820 vs. ExPASy TrEMBL
Match: A0A6J1DBU6 (myosin-2 heavy chain, non muscle-like OS=Momordica charantia OX=3673 GN=LOC111018880 PE=4 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 3.0e-177
Identity = 389/627 (62.04%), Postives = 471/627 (75.12%), Query Frame = 0

Query: 1   MAKKKPTRSAKELKQTPNN-QEETSDSEQPKSAMDDD-SKLQSLKSLNERLLKEMVEKRV 60
           MAKKK TR AKE KQ  N  Q+E SD EQ ++AMDD   KLQSLKSLN+RL+KE  E+R+
Sbjct: 1   MAKKKATRVAKEPKQMQNQPQQEDSDPEQSRAAMDDSVLKLQSLKSLNDRLVKETHERRM 60

Query: 61  EVGDLVQSKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRIQEMGG 120
           EVG LV++K+ALE+DLKRNV+EK QVMGEL EA +G+YGL+LE+NVV V+LQS+++EMGG
Sbjct: 61  EVGALVKTKDALEIDLKRNVDEKNQVMGELGEACEGIYGLDLEKNVVSVFLQSQMEEMGG 120

Query: 121 GICGLL----ESERVKGLEIRNLKAEITGLVSEVGEEREKWRGVCCERDEIKVEFDGLLK 180
           GICGL+    ESER+K +EI  LKAE+  LV +V EEREKWR V  ERD +K+ FDGLL+
Sbjct: 121 GICGLMAEKRESERLKEMEIGLLKAEVNELVLKVEEEREKWRRVRSERDAMKIAFDGLLE 180

Query: 181 ETGDLRGKVVEMERNERRALEEIDDLKGKCKKLLSEKKECEILNANLTKDNELIKKLLEE 240
           ETGDLRGK   MERNER ALEEI  LKGKC+KL+ EK E E++N  L K+NE +KKLL+E
Sbjct: 181 ETGDLRGK---MERNERMALEEIAGLKGKCEKLMGEKMEIEVVNGTLLKENESVKKLLDE 240

Query: 241 SGRVIEDLERKVDVKMKEKGEIEKEKNGLEMEVEKLEKEVTQLKQSTFCLKQEKEENGMR 300
           S  VIEDLERK++ KMKEK EIE+EK+GL+ME+ KLEKEV QL +STF  KQEK+EN  R
Sbjct: 241 SAVVIEDLERKMEEKMKEKVEIEREKDGLQMEILKLEKEVVQLNESTFSFKQEKKENEQR 300

Query: 301 IYELQMRNEEALVKESGMLMECDLLVKELQKKEK-------------------------- 360
           I E++ R EEA+ KE+GMLME D LVK+LQKKEK                          
Sbjct: 301 ISEIEKRTEEAIEKENGMLMEIDALVKQLQKKEKDMEMLTQQRESLELNLNLVQEEVGNL 360

Query: 361 ------------AMEKAKTEAQNIIGDLQKESSKLKEAIASLTKMSDVGKARNEELINQI 420
                        ME+ K EA+NIIG+LQ+ESSKLKEAI SLT++ DV KARNEEL+++I
Sbjct: 361 RRTIEVITRDKVEMEEKKKEAENIIGELQRESSKLKEAITSLTELGDVEKARNEELLSEI 420

Query: 421 GRLRDTLDEVSFERDDARKRFGDEKEKVEKLSLLLKDKERRIEEAIKEVEKAKIAQEEES 480
            RLRD L EVSFERDDARK F DEK  VEKLSLLLKDKE R+ EA        +  +E+S
Sbjct: 421 SRLRDALVEVSFERDDARKSFSDEKGSVEKLSLLLKDKESRLVEA--------MISQEDS 480

Query: 481 LNVKKEMERRINALIGERELMEKNLLAAKSRIDELKAKVNSAVCNSEKALSLLKKTRLTV 540
           LN+KKEME+RI+ L+GER+ MEKNLL A+ RID+LKA+V SAV NSEKAL+LLKKT L V
Sbjct: 481 LNIKKEMEKRIDILVGERDSMEKNLLEAQRRIDDLKAEVKSAVANSEKALALLKKTCLAV 540

Query: 541 CDGYGKREVEEVSSDEHKVGKEMQPFVEHLDAIKTSFTNKEKAVEEMTRVLETERMEQQK 584
           CDGY ++ V E SS         +PFVEHL+AI+TSFTNKEK VEEM   LET R+E ++
Sbjct: 541 CDGY-EKGVGEASS---------EPFVEHLNAIRTSFTNKEKMVEEMKHRLETARVE-ER 600

BLAST of CsaV3_2G004820 vs. ExPASy TrEMBL
Match: A0A6J1FD80 (polyamine-modulated factor 1-binding protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111444320 PE=4 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 2.1e-170
Identity = 382/626 (61.02%), Postives = 451/626 (72.04%), Query Frame = 0

Query: 1   MAKKKPTRSAKELKQTPNNQEETSDSEQP--KSAMDDD---SKLQSLKSLNERLLKEMVE 60
           MAKKKPTRS  E K+ P++QEE  DSEQ   KSA+D+    S+LQSLKSLNERLLKE  E
Sbjct: 1   MAKKKPTRSGNEPKKVPDHQEENRDSEQQLLKSAVDESVEKSQLQSLKSLNERLLKETHE 60

Query: 61  KRVEVGDLVQSKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVYLQSRIQE 120
           KR E G LVQ+KE LELDLK+N +EK+QVM ELS A DGV GLELERNVV VYLQ++++E
Sbjct: 61  KRKEFGALVQAKEGLELDLKKNADEKKQVMCELSVACDGVSGLELERNVVHVYLQTQMEE 120

Query: 121 MGGGICGLLESERVKGLEIRNLKAEITGLVSEVGEEREKWRGVCCERDEIKVEFDGLLKE 180
           MGG IC L+ESERVK +EI  LK E  GLV +V EEREKW  VCCERD IK +FDGL +E
Sbjct: 121 MGGVICRLVESERVKNVEIGRLKGENNGLVLKVEEEREKWMKVCCERDGIKADFDGLFEE 180

Query: 181 TGDLRGKVVEMERNERRALEEIDDLKGKCKKLLSEKKECEILNANLTKDNELIKKLLEES 240
           TGDLR K+VEME+NERRALEEI+DLK KCKKL  EK E E++N NL K+ EL+K+LL+ES
Sbjct: 181 TGDLRRKMVEMEKNERRALEEIEDLKVKCKKLSGEKMESEVMNGNLLKEKELVKRLLDES 240

Query: 241 GRVIEDLERKVDVKMKEKGEIEKEKNGLEMEVEKLEKEVTQLKQSTFCLKQEKEENGMRI 300
           GRVIEDLERKVD+K KEK E+EKEK  LEME+E+L KEV +L +S+F LKQEKEENG  I
Sbjct: 241 GRVIEDLERKVDLKTKEKVEVEKEKKALEMEIERLGKEVAELNESSFGLKQEKEENGKVI 300

Query: 301 YELQMRNEEALVKESGMLMECDLLVKELQKKEK--------------------------- 360
            EL  R EEA+ KESG+LME D LVKELQ+KEK                           
Sbjct: 301 SELVKRIEEAVEKESGLLMEVDGLVKELQRKEKDVEMLTQQRDSVNVNLNRVEQEALSLR 360

Query: 361 -----------AMEKAKTEAQNIIGDLQKESSKLKEAIASLTKMSDVGKARNEELINQIG 420
                       ME+AK E +N++ DLQ+ESSKLKEA+ SLT+   V KARNEEL++Q+G
Sbjct: 361 RTIEIITREKAKMEEAKVEVENVVVDLQRESSKLKEAVTSLTESRSVEKARNEELLSQMG 420

Query: 421 RLRDTLDEVSFERDDARKRFGDEKEKVEKLSLLLKDKERRIEEAIKEVEKAKIAQEEESL 480
            LR  L+ VS ERD              KL LLL+DKE+RIEEA+ E+EK K A+  ES+
Sbjct: 421 CLRKALNGVSLERD--------------KLGLLLEDKEKRIEEAMVELEKTKTAR-VESM 480

Query: 481 NVKKEMERRINALIGERELMEKNLLAAKSRIDELKAKVNSAVCNSEKALSLLKKTRLTVC 540
           NV KE ERRI  L+GER+ MEK+LL A+SRIDELK KV SAV +SEKAL+LLK+T L+VC
Sbjct: 481 NVAKERERRIEVLVGERDRMEKSLLEAESRIDELKGKVKSAVDDSEKALALLKETCLSVC 540

Query: 541 DGYGKREVEEVSSDEHKVGKEMQPFVEHLDAIKTSFTNKEKAVEEMTRVLETERMEQQKK 584
           DGY K E             E + FVEHLDAIK SF NKEK V EM + LET R E++KK
Sbjct: 541 DGYEKEE------------GESKAFVEHLDAIKASFMNKEKMVGEMKQSLETARAEERKK 599

BLAST of CsaV3_2G004820 vs. TAIR 10
Match: AT5G27330.1 (Prefoldin chaperone subunit family protein )

HSP 1 Score: 195.7 bits (496), Expect = 1.1e-49
Identity = 193/633 (30.49%), Postives = 328/633 (51.82%), Query Frame = 0

Query: 1   MAKKKPTR----SAKELKQTPNNQEETSDSEQPK----SAMDD----DSKLQSLKSLNER 60
           MAKKK +R    ++ E +Q  N     +  +  K    S+M+D    + K Q+LKSLN  
Sbjct: 1   MAKKKVSRNSNGASNEQQQIQNQSVPVTSQKSTKLSRESSMEDHDSSEEKFQNLKSLNAI 60

Query: 61  LLKEMVEKRVEVGDLVQSKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVVCVY 120
           LLK+ +EKR ++  L Q+K++LE++L R+  EK  +  EL  + D  + L++E +++  +
Sbjct: 61  LLKQTMEKRQQIESLFQAKDSLEIELVRSGKEKTLLREELCGSSDENFMLKIEMDLLMGF 120

Query: 121 LQSRIQEMGGGICGLLESERVKGLEIRNLKAEITGLVSEVGEEREKWRGVCCERDEIKVE 180
           ++ R++EMG  +  L + +  +  EIR+LK E  GL+ ++  ERE++  VC ERD +K  
Sbjct: 121 VEGRVKEMGVEVDWLFKEKSDRETEIRDLKREANGLIRKLESEREEFSRVCDERDLVKSG 180

Query: 181 FDGLLKETGDLRGKVVEMERNERRALEEIDDLKGKCKKLLSEKKECEILNANLTKDNELI 240
           FD   +E   L+  VV +E  E    EE+  LK +  +L+ E+K+ E +     ++   +
Sbjct: 181 FDLQSEEMNLLKESVVRLEMREVSLGEEVGRLKCENGRLVKERKKREEVIERGNRERSEL 240

Query: 241 KKLLEESGRVIEDLERKVDVKMKEKGEIEKEKNGLEMEVEKLEKEVTQLKQSTFCLKQEK 300
            + LEE  R I+ L+R+++  +KEK E+E  +      + +LEK++  + +    L +E+
Sbjct: 241 VESLEEKVREIDVLKREIEGVVKEKMEVEMVRRDQREMIVELEKKLGDMNEIVESLTKER 300

Query: 301 ------------------EENGMRIYELQMRNEEALVKES---GMLMECDLLVKELQ--- 360
                             EE   R  ++    +E  VKES   G+++E + + KE++   
Sbjct: 301 EGLRGQVVGLEKSLDEVTEEAKARAEQINELVKEKTVKESELEGLMVENNSIKKEIEMAM 360

Query: 361 ----KKEKAMEKAKTEAQNI----------IGDLQKESSKLKEAIASLTKMSDVGKARNE 420
                KEK +E+   E   +          I +L K + + K A+A L K  +      E
Sbjct: 361 VQFSDKEKLVEQLLREKNELVQRVVNQEAEIVELSKLAGEQKHAVAQLRKDYNDQIKNGE 420

Query: 421 ELINQIGRLRDTLDEVSFERDDARKRFGDEKEKVEKLSLLLKDKERRIEEAIKEVEKAKI 480
           +L   + +L+D L  V  ERD+A K   +EK  +  L   +   E+  E   KE+EK K 
Sbjct: 421 KLNCNVSQLKDALALVEVERDNAGKALDEEKRNMVALKEKVVALEKTNEATGKELEKIK- 480

Query: 481 AQEEESLNVKKEMERRINALIGERELMEKNLLAAKSRIDELKAKVNSAVCNSEKALSLLK 540
           A+    +  KKE+E R  +L  E+ +++K+++  K     LK ++ SA  N++++L++LK
Sbjct: 481 AERGRLIKEKKELENRSESLRNEKAILQKDIVELKRATGVLKTELESAGTNAKQSLTMLK 540

Query: 541 KTRLTVCDGYGKREVEEVSSDEHKVGKEMQPFVEHLDAIKTSFTNKEKAVEEMTRVLETE 581
                VC    K+       DE K GK M  +   L+AIK +F NKE  VEEM + L   
Sbjct: 541 SVSSLVCGIENKK-------DEKKRGKGMDSYSVQLEAIKKAFKNKESMVEEMKKELAKM 600

BLAST of CsaV3_2G004820 vs. TAIR 10
Match: AT3G05130.1 (BEST Arabidopsis thaliana protein match is: Prefoldin chaperone subunit family protein (TAIR:AT5G27330.1); Has 171032 Blast hits to 82560 proteins in 3364 species: Archae - 2835; Bacteria - 33550; Metazoa - 71995; Fungi - 13365; Plants - 9598; Viruses - 592; Other Eukaryotes - 39097 (source: NCBI BLink). )

HSP 1 Score: 193.0 bits (489), Expect = 6.9e-49
Identity = 181/639 (28.33%), Postives = 334/639 (52.27%), Query Frame = 0

Query: 1   MAKKKPTRSAKELKQTPNNQEETSDSEQP-----------KSAMDD----DSKLQSLKSL 60
           MAKKK +R++ +     +N+++  D + P           +S+M++    + + Q+LKSL
Sbjct: 1   MAKKKASRNSNDTAN--DNRQQQHDQKAPETDKKATVLSRQSSMEEHDSSEEQFQNLKSL 60

Query: 61  NERLLKEMVEKRVEVGDLVQSKEALELDLKRNVNEKEQVMGELSEARDGVYGLELERNVV 120
           N  LLK+ +EKR ++  LVQ+K+ LE +L R   EK  +  EL +  D  +GL+ E + V
Sbjct: 61  NAMLLKQAMEKRNQIDSLVQAKDELETELARYCQEKTGLRDELDQVSDENFGLKFELDFV 120

Query: 121 CVYLQSRIQEMGGGICGLLESERVKGLEIRNLKAEITGLVSEVGEEREKWRGVCCERDEI 180
            V+++S+ +EM  G+  L++ +  +  EIR LK E   L  +V  E+E+ R VC ERD I
Sbjct: 121 IVFVESQFREMCVGVDMLVKEKSDRESEIRVLKGEAIELTGKVEIEKEQLRKVCDERDLI 180

Query: 181 KVEFDGLLKETGDLRGKVVEMERNERRALEEIDDLKGKCKKLLSEKKECEILNANLTKDN 240
           K  FD   +E   L+  VV +E  E      I  L+ + ++L+ E+K  E     + K+ 
Sbjct: 181 KNGFDLQHEEVNRLKECVVRLEEKESNLEIVIGKLESENERLVKERKVREEEIEGVKKEK 240

Query: 241 ELIKKLLEESGRVIEDLERKVDVKMKEKGEIEKEKNGLEMEVEKLEKEVTQLKQSTFCLK 300
             ++K++EE    I+ L+R++ V + EK E+E  K   +  +E+LE+++ +L ++   L 
Sbjct: 241 IGLEKIMEEKKNEIDGLKREIKVLLSEKNEMEIVKIEQKGVIEELERKLDKLNETVRSLT 300

Query: 301 QEKEENGMRIYELQMRNEEALVKESGMLMECDLLVKELQKKEKAMEK------------- 360
           +E++     +  L+   +E++ KESGM++E D L KE   KE  +E+             
Sbjct: 301 KEEKVLRDLVIGLEKNLDESMEKESGMMVEIDALGKERTIKESEVERLIGEKNLIEKQME 360

Query: 361 ----AKTEAQNIIGDLQKESSKLKE---------------------AIASLTKMSDVGKA 420
                 ++   +I  L +E  +L+E                     A+A L K  D    
Sbjct: 361 MLNVQSSDKGKLIDQLSREKVELEERIFSRERKLVELNRKADELTHAVAVLQKNCDDQTK 420

Query: 421 RNEELINQIGRLRDTLDEVSFERDDARKRFGDEKEKVEKLSLLLKDKERRIEEAIKEVEK 480
            N +L  ++ +L + L +V   R++A K   +EK   E L   +   E+ + + ++E+EK
Sbjct: 421 INGKLSCKVDQLSNALAQVELRREEADKALDEEKRNGEDLKAEVLKSEKMVAKTLEELEK 480

Query: 481 AKIAQEEESLNVKKEMERRINALIGERELMEKNLLAAKSRIDELKAKVNSAVCNSEKALS 540
            KI + +   + K ++E +  +L  E   +EK L+  +  ++ LK ++ SA  ++++++ 
Sbjct: 481 VKI-ERKSLFSAKNDLESQSESLKSENVKLEKELVELRKAMEALKTELESAGMDAKRSMV 540

Query: 541 LLKKTRLTVCDGYGKREVEEVSSDEHKVGKEMQPFVEHLDAIKTSFTNKEKAVEEMTR-- 584
           +L K+  ++      RE + + S+E K     +P+   L++I+ +F NKE  +EEM +  
Sbjct: 541 ML-KSAASMLSQLENRE-DRLISEEQKREIGTEPYAMELESIEKAFKNKEDIIEEMKKEA 600