CsaV3_1G046460 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_1G046460
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	Peptidase A1 domain-containing protein
Location	chr1: 32296925 .. 32298510 (+)
RNA-Seq Expression	CsaV3_1G046460
Synteny	CsaV3_1G046460

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACATCACCAACAGCAACAAAAAGAGAAAGATGCCTTCGATATCATCAATCTCCACTGCAACAAAATTCTTGAGCCTATTCCTTCTTCTTGTACATGTCTCAACACAAACCCTAGCAACAAACCCTAAAACCAATTTCCCCAAAGATTCTCTAGTTCTTGGTCTTGTTCATTCAAGAACATCCCTCCTTACACCCAAAAAAGGCTATAATTTCATTTCAAAGAAGAGAATGAAGGCAATGGATCAGACGGATGGTGATGATAATGTGATAGAGCCATTGAGAGAAATTAGGGATGGTTATTTAATGTCCTTATCAATAGGGACACCCCCACAAGTTGTTCAAGTGTATATGGACACTGGAAGTGATCTCACGTGGGTTCCTTGTGGTAACCTCTCTTTTGATTGTCAAGATTGTGAGGAGTATCAAAACAATATTTCTGGCCCAAGATTGGCCGCCTTTTTGCCTACTCATTCTTCTACTTCTATTCGAGACACGTGTGGTAGTTCCTTTTGTATGGATATTCATAGCTCTGATAACCCTTTTGATCCTTGCACAATTGCTGGTTGTTCCTTAGCTAGCCTTGTGAAGGGCACTTGCCCTAGACCTTGCCCTTCTTTTGCTTATACTTATGGAGCAAGTGGGGTTGTAACTGGAAGTTTAACAAGAGATGTTCTTTTTACGCATGGAAATTATAATAACAATAATAACAATAATAAGCAAATCCCTAGGTTTTGTTTTGGATGTGTTGGAGCAACTTATAGAGAGCCAATTGGGATTGCTGGTTTTGGAAGAGGCTTACTTTCTCTTCCTTTTCAATTAGGGTTTTCTCATAAGGGGTTTTCTCATTGCTTTTTACCTTTTAAATTCTCAAATAACCCTAATTTCTCAAGCCCTTTGATTCTTGGTAATCTTGCCATTTCTTCAAAAGATGAAAATTTGCAATTTACCCCTTTGTTGAAAAGTCCAATGTACCCTAACTATTACTATATTGGGCTTGAGTCAATTACCATTGGGAATGGGGATAATAATTTTAGATTTGGGGTTTCTTTTAAATTGAGAGAGATTGATACAAAGGGTAATGGAGGGATGTTGATTGATTCTGGTACTACTTATACTCATTTACCTGAACCATTGTATTCACAACTTATTTCTAATCTTGAATTAGTGATAGGTTATCCAAGAGCCAAGCAAGTTGAACTCAATACTGGGTTTGATCTTTGTTATAAAGTTCCTTGTAAAAACAACAATTCTTCTTTTGTTGATGACGCTCAACTCCCTTCTATAACATTCCATTTTTTGAATAATGTCAGTGTTGTTTTGCCTCAAGGCAATAACTTCTATGCCATGGCTGCTCCAATTAACTCCACTGTGGTTAAATGTTTGTTGTATCAAAGCATGGACGGTGTTGGTGACGATAACGACAGTGACGACAATGGGCCGGCGGGTATTTTCGGAAGCTTTCAACAGCAAAATATAGAGGTCGTTTATGATTTGGAGAAGGAAAGATTAGGGTTTCAACCAATGGATTGTGTTTCTGTTGCTGCCAAACAGGGACTTCACAAGAATGTTAGAAGGAATGAAAGTTGA

mRNA sequence

ATGCCTTCGATATCATCAATCTCCACTGCAACAAAATTCTTGAGCCTATTCCTTCTTCTTGTACATGTCTCAACACAAACCCTAGCAACAAACCCTAAAACCAATTTCCCCAAAGATTCTCTAGTTCTTGGTCTTGTTCATTCAAGAACATCCCTCCTTACACCCAAAAAAGGCTATAATTTCATTTCAAAGAAGAGAATGAAGGCAATGGATCAGACGGATGGTGATGATAATGTGATAGAGCCATTGAGAGAAATTAGGGATGGTTATTTAATGTCCTTATCAATAGGGACACCCCCACAAGTTGTTCAAGTGTATATGGACACTGGAAGTGATCTCACGTGGGTTCCTTGTGGTAACCTCTCTTTTGATTGTCAAGATTGTGAGGAGTATCAAAACAATATTTCTGGCCCAAGATTGGCCGCCTTTTTGCCTACTCATTCTTCTACTTCTATTCGAGACACGTGTGGTAGTTCCTTTTGTATGGATATTCATAGCTCTGATAACCCTTTTGATCCTTGCACAATTGCTGGTTGTTCCTTAGCTAGCCTTGTGAAGGGCACTTGCCCTAGACCTTGCCCTTCTTTTGCTTATACTTATGGAGCAAGTGGGGTTGTAACTGGAAGTTTAACAAGAGATGTTCTTTTTACGCATGGAAATTATAATAACAATAATAACAATAATAAGCAAATCCCTAGGTTTTGTTTTGGATGTGTTGGAGCAACTTATAGAGAGCCAATTGGGATTGCTGGTTTTGGAAGAGGCTTACTTTCTCTTCCTTTTCAATTAGGGTTTTCTCATAAGGGGTTTTCTCATTGCTTTTTACCTTTTAAATTCTCAAATAACCCTAATTTCTCAAGCCCTTTGATTCTTGGTAATCTTGCCATTTCTTCAAAAGATGAAAATTTGCAATTTACCCCTTTGTTGAAAAGTCCAATGTACCCTAACTATTACTATATTGGGCTTGAGTCAATTACCATTGGGAATGGGGATAATAATTTTAGATTTGGGGTTTCTTTTAAATTGAGAGAGATTGATACAAAGGGTAATGGAGGGATGTTGATTGATTCTGGTACTACTTATACTCATTTACCTGAACCATTGTATTCACAACTTATTTCTAATCTTGAATTAGTGATAGGTTATCCAAGAGCCAAGCAAGTTGAACTCAATACTGGGTTTGATCTTTGTTATAAAGTTCCTTGTAAAAACAACAATTCTTCTTTTGTTGATGACGCTCAACTCCCTTCTATAACATTCCATTTTTTGAATAATGTCAGTGTTGTTTTGCCTCAAGGCAATAACTTCTATGCCATGGCTGCTCCAATTAACTCCACTGTGGTTAAATGTTTGTTGTATCAAAGCATGGACGGTGTTGGTGACGATAACGACAGTGACGACAATGGGCCGGCGGGTATTTTCGGAAGCTTTCAACAGCAAAATATAGAGGTCGTTTATGATTTGGAGAAGGAAAGATTAGGGTTTCAACCAATGGATTGTGTTTCTGTTGCTGCCAAACAGGGACTTCACAAGAATGTTAGAAGGAATGAAAGTTGA

Coding sequence (CDS)

ATGCCTTCGATATCATCAATCTCCACTGCAACAAAATTCTTGAGCCTATTCCTTCTTCTTGTACATGTCTCAACACAAACCCTAGCAACAAACCCTAAAACCAATTTCCCCAAAGATTCTCTAGTTCTTGGTCTTGTTCATTCAAGAACATCCCTCCTTACACCCAAAAAAGGCTATAATTTCATTTCAAAGAAGAGAATGAAGGCAATGGATCAGACGGATGGTGATGATAATGTGATAGAGCCATTGAGAGAAATTAGGGATGGTTATTTAATGTCCTTATCAATAGGGACACCCCCACAAGTTGTTCAAGTGTATATGGACACTGGAAGTGATCTCACGTGGGTTCCTTGTGGTAACCTCTCTTTTGATTGTCAAGATTGTGAGGAGTATCAAAACAATATTTCTGGCCCAAGATTGGCCGCCTTTTTGCCTACTCATTCTTCTACTTCTATTCGAGACACGTGTGGTAGTTCCTTTTGTATGGATATTCATAGCTCTGATAACCCTTTTGATCCTTGCACAATTGCTGGTTGTTCCTTAGCTAGCCTTGTGAAGGGCACTTGCCCTAGACCTTGCCCTTCTTTTGCTTATACTTATGGAGCAAGTGGGGTTGTAACTGGAAGTTTAACAAGAGATGTTCTTTTTACGCATGGAAATTATAATAACAATAATAACAATAATAAGCAAATCCCTAGGTTTTGTTTTGGATGTGTTGGAGCAACTTATAGAGAGCCAATTGGGATTGCTGGTTTTGGAAGAGGCTTACTTTCTCTTCCTTTTCAATTAGGGTTTTCTCATAAGGGGTTTTCTCATTGCTTTTTACCTTTTAAATTCTCAAATAACCCTAATTTCTCAAGCCCTTTGATTCTTGGTAATCTTGCCATTTCTTCAAAAGATGAAAATTTGCAATTTACCCCTTTGTTGAAAAGTCCAATGTACCCTAACTATTACTATATTGGGCTTGAGTCAATTACCATTGGGAATGGGGATAATAATTTTAGATTTGGGGTTTCTTTTAAATTGAGAGAGATTGATACAAAGGGTAATGGAGGGATGTTGATTGATTCTGGTACTACTTATACTCATTTACCTGAACCATTGTATTCACAACTTATTTCTAATCTTGAATTAGTGATAGGTTATCCAAGAGCCAAGCAAGTTGAACTCAATACTGGGTTTGATCTTTGTTATAAAGTTCCTTGTAAAAACAACAATTCTTCTTTTGTTGATGACGCTCAACTCCCTTCTATAACATTCCATTTTTTGAATAATGTCAGTGTTGTTTTGCCTCAAGGCAATAACTTCTATGCCATGGCTGCTCCAATTAACTCCACTGTGGTTAAATGTTTGTTGTATCAAAGCATGGACGGTGTTGGTGACGATAACGACAGTGACGACAATGGGCCGGCGGGTATTTTCGGAAGCTTTCAACAGCAAAATATAGAGGTCGTTTATGATTTGGAGAAGGAAAGATTAGGGTTTCAACCAATGGATTGTGTTTCTGTTGCTGCCAAACAGGGACTTCACAAGAATGTTAGAAGGAATGAAAGTTGA

Protein sequence

MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYNFISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNYNNNNNNNKQIPRFCFGCVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKDENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQNIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNVRRNES*

Homology

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BLAST of CsaV3_1G046460 vs. NCBI nr
Match: XP_004145478.2 (probable aspartyl protease At4g16563 [Cucumis sativus] >KGN66888.1 hypothetical protein Csa_007266 [Cucumis sativus])

HSP 1 Score: 1067.4 bits (2759), Expect = 3.9e-308
Identity = 518/518 (100.00%), Postives = 518/518 (100.00%), Query Frame = 0


Query: 1   MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN
Sbjct: 1   MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120
           FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNYNNNNNNNKQIPRFCFGCVG 240
           LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNYNNNNNNNKQIPRFCFGCVG
Sbjct: 181 LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNYNNNNNNNKQIPRFCFGCVG 240

Query: 241 ATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKD 300
           ATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKD
Sbjct: 241 ATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKD 300

Query: 301 ENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTT 360
           ENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTT
Sbjct: 301 ENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTT 360

Query: 361 YTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSITF 420
           YTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSITF
Sbjct: 361 YTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSITF 420

Query: 421 HFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQ 480
           HFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQ
Sbjct: 421 HFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQ 480

Query: 481 NIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNVRRNES 519
           NIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNVRRNES
Sbjct: 481 NIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNVRRNES 518

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BLAST of CsaV3_1G046460 vs. NCBI nr
Match: XP_008459091.1 (PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis melo] >KAA0043307.1 aspartic proteinase nepenthesin-2 [Cucumis melo var. makuwa] >TYK29371.1 aspartic proteinase nepenthesin-2 [Cucumis melo var. makuwa])

HSP 1 Score: 1019.2 bits (2634), Expect = 1.2e-293
Identity = 495/520 (95.19%), Postives = 505/520 (97.12%), Query Frame = 0


Query: 1   MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           MPSISS S ATKFLSLFLLLVH S QTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN
Sbjct: 1   MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120
           FISKKRMKAMDQ DGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNNISGP+LAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNY----NNNNNNNKQIPRFCF 240
           LA+LVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLF HGNY    NNN+NNNKQ+PRFCF
Sbjct: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF 240

Query: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAI 300
           GCVGATYREPIGIAGFGRGLLSLPFQLGFS KGFSHCFLPFKFSNNPNFSSPLILG+LAI
Sbjct: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI 300

Query: 301 SSKDENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLID 360
           SSKDENLQFTPLLKSP+YPNYYYIGLESITIGNG+NNFRFGVSFKLREIDTKGNGGMLID
Sbjct: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360

Query: 361 SGTTYTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLP 420
           SGTTYTHLPEPLYSQLISNLE VI YPRAKQVELNTGFDLCYKVPCKNNNSSFVDD+QLP
Sbjct: 361 SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420

Query: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480
           SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS
Sbjct: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480

Query: 481 FQQQNIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNVRRN 517
           FQQQN++VVYDLEKERLGFQ MDCVSVAA QGLHKNVRRN
Sbjct: 481 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNVRRN 520

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BLAST of CsaV3_1G046460 vs. NCBI nr
Match: XP_038893627.1 (probable aspartyl protease At4g16563 [Benincasa hispida])

HSP 1 Score: 927.2 bits (2395), Expect = 6.4e-266
Identity = 451/514 (87.74%), Postives = 480/514 (93.39%), Query Frame = 0


Query: 1   MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           M SIS+ S A K LS FLLLV+VS +TLATNPKTN PKDSLV+GLVHSRT+LLTPKKGYN
Sbjct: 1   MASIST-SFAKKILSYFLLLVYVSRKTLATNPKTNGPKDSLVIGLVHSRTTLLTPKKGYN 60

Query: 61  FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120
           FIS+KRMKAM+    DDNVIEPLREIRDGYLMSL++GTPPQV+QVYMDTGSDLTWVPCGN
Sbjct: 61  FISRKRMKAMEM---DDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNN+SGP+LAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTH-GNYNNNNNNNKQIPRFCFGCV 240
           LA+LVK TCPRPCPSFAYTYGASGVV G+LTRDVL  H  N N+ N++ K+ PRFCFGCV
Sbjct: 181 LATLVKATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCV 240

Query: 241 GATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK 300
           GA+YREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLA+SSK
Sbjct: 241 GASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSK 300

Query: 301 DENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGT 360
           DE+LQFTPLLKSP+YPNYYYIGLESITIGNG++NFRFGVSF LREIDTKGNGGMLIDSGT
Sbjct: 301 DEHLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGT 360

Query: 361 TYTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSIT 420
           TYTHLPEPLYSQLISNLE VI YPRAKQVELNTGFDLCYKVPCKNNN SF+DD+QLPSIT
Sbjct: 361 TYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSIT 420

Query: 421 FHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQ 480
           FHFLNNVSVVLPQ NNFYAMAAPINSTVVKCLL+QSMDGVG D D D +GPAGIFGSFQQ
Sbjct: 421 FHFLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQ 480

Query: 481 QNIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNV 514
           QN+EVVYDLEKERLGFQPMDC  VAA QGLHKNV
Sbjct: 481 QNLEVVYDLEKERLGFQPMDCAYVAATQGLHKNV 510

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BLAST of CsaV3_1G046460 vs. NCBI nr
Match: XP_023520027.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 832.4 bits (2149), Expect = 2.1e-237
Identity = 412/506 (81.42%), Postives = 454/506 (89.72%), Query Frame = 0


Query: 10  ATKFLSLFLLLVHVS----TQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYNFISKK 69
           A  F  L L+LV VS     QTLA NPKT F KDSLVLGLVHSRTSLLTPK+GYN +S+K
Sbjct: 6   AKSFFVLVLVLVLVSGEAMGQTLA-NPKTKFLKDSLVLGLVHSRTSLLTPKRGYNSLSRK 65

Query: 70  RMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDC 129
           R+K M+   G+D+VIEPLREIRDGYLMSL++GTPPQV+QVYMDTGSDLTWVPCGNLSFDC
Sbjct: 66  RIKPMEM--GNDDVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDC 125

Query: 130 QDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLASLV 189
           QDC+EYQNN+ GP+LAAFLPTHSSTSIRDTCGSSFC+DIHSSDNPFDPCTIAGCSLA+LV
Sbjct: 126 QDCDEYQNNVLGPKLAAFLPTHSSTSIRDTCGSSFCIDIHSSDNPFDPCTIAGCSLATLV 185

Query: 190 KGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNYNNNNNNNKQIPRFCFGCVGATYRE 249
           KGTCPRPCPSF+YTYGASG+V G+LT+DV+F HG   N+ N++++IP+FCFGCVGATYRE
Sbjct: 186 KGTCPRPCPSFSYTYGASGLVIGTLTKDVIFIHG---NSPNSSRKIPKFCFGCVGATYRE 245

Query: 250 PIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKDENLQF 309
           PIGIAGFGRGLLSLP+QLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK E+L+F
Sbjct: 246 PIGIAGFGRGLLSLPYQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK-EHLKF 305

Query: 310 TPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLP 369
           TPLLKSP YPNYYYIGLESITIGNG+N  RFGVS +LREIDTKGNGG+LIDSGTTYTHLP
Sbjct: 306 TPLLKSPFYPNYYYIGLESITIGNGENYSRFGVSLQLREIDTKGNGGILIDSGTTYTHLP 365

Query: 370 EPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSITFHFLNN 429
           EPLYSQLISNLE +I YPRAK+ ELNTGFDLCYKVP K NN+ F D+ +LPSITFHFLNN
Sbjct: 366 EPLYSQLISNLESLISYPRAKEHELNTGFDLCYKVPYK-NNTFFSDEFELPSITFHFLNN 425

Query: 430 VSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQNIEVV 489
           VSVVLPQGN+FYAMAAP NSTVVKCLL+QSMDG GD       GPAGIFGSFQQQN+EVV
Sbjct: 426 VSVVLPQGNSFYAMAAPSNSTVVKCLLFQSMDGDGD-------GPAGIFGSFQQQNLEVV 485

Query: 490 YDLEKERLGFQPMDCVSVAAKQGLHK 512
           YDLEKERLGF+ MDC SVA  QGLHK
Sbjct: 486 YDLEKERLGFEGMDCASVAVSQGLHK 496

[Close]

BLAST of CsaV3_1G046460 vs. NCBI nr
Match: KAG6583807.1 (putative aspartyl protease, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 828.2 bits (2138), Expect = 4.0e-236
Identity = 412/513 (80.31%), Postives = 456/513 (88.89%), Query Frame = 0


Query: 1   MPSISSISTATKFLSLFLLLV--HVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKG 60
           M SI++ S     L L L+LV      QTLA NPKT F KDSLVLGLVHSRTSLLTPK+G
Sbjct: 1   MASIAARSFFVLVLVLVLVLVSGEAMGQTLA-NPKTKFLKDSLVLGLVHSRTSLLTPKRG 60

Query: 61  YNFISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPC 120
           YN +S+KR+K M+   G+D+VIEPLREIRDGYLMSL++GTPPQV+QVYMDTGSDLTWVPC
Sbjct: 61  YNSLSRKRIKPMEM--GNDDVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPC 120

Query: 121 GNLSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAG 180
           GNLSFDCQDC+EYQNN+ GP+LAAFLPTHSSTSIRDTCGSSFC+DIHSSDNPFDPCTIAG
Sbjct: 121 GNLSFDCQDCDEYQNNVLGPKLAAFLPTHSSTSIRDTCGSSFCIDIHSSDNPFDPCTIAG 180

Query: 181 CSLASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNYNNNNNNNKQIPRFCFGC 240
           CSLA+LVKGTCPRPCPSF+YTYGASG+V G+LT+DV+F HG   N+ N++++IP+FCFGC
Sbjct: 181 CSLATLVKGTCPRPCPSFSYTYGASGLVIGTLTKDVIFIHG---NSPNSSRKIPKFCFGC 240

Query: 241 VGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISS 300
           VGATYREPIGIAGFGRGLLSLP QLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISS
Sbjct: 241 VGATYREPIGIAGFGRGLLSLPSQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISS 300

Query: 301 KDENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSG 360
           K E+L+FTPLLKSP YPNYYYIGLESITIGNG+N  RFGVS +LREIDTKGNGG+LIDSG
Sbjct: 301 K-EHLKFTPLLKSPFYPNYYYIGLESITIGNGENYSRFGVSLQLREIDTKGNGGILIDSG 360

Query: 361 TTYTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSI 420
           TTYTHLPEPLYSQ+ISNLE +I YPRAK+ ELNTGFDLCYKVP K NN+ F D+ +LPSI
Sbjct: 361 TTYTHLPEPLYSQIISNLESLISYPRAKEHELNTGFDLCYKVPYK-NNTFFSDEFELPSI 420

Query: 421 TFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQ 480
           TFHFLNNVSVVLPQGN+FYAMAAP NSTVVKCLL+QSMDG GD       GPAGIFGSFQ
Sbjct: 421 TFHFLNNVSVVLPQGNSFYAMAAPSNSTVVKCLLFQSMDGDGD-------GPAGIFGSFQ 480

Query: 481 QQNIEVVYDLEKERLGFQPMDCVSVAAKQGLHK 512
           QQN+EVVYDLEKERLGF+ MDC SVA  QGLHK
Sbjct: 481 QQNLEVVYDLEKERLGFEGMDCASVAVSQGLHK 498

[Close]

BLAST of CsaV3_1G046460 vs. ExPASy Swiss-Prot
Match: Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 2.3e-53
Identity = 150/447 (33.56%), Postives = 204/447 (45.64%), Query Frame = 0


Query: 90  YLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNISGPRLAAFLPTHSS 149
           YL+SLS+G+    V +Y+DTGSDL W PC    F C  CE      S P       + SS
Sbjct: 83  YLISLSVGSSSSAVSLYLDTGSDLVWFPC--RPFTCILCESKPLPPSPP------SSLSS 142

Query: 150 TSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLASLVKGTC---PRPCPSFAYTYGASGVV 209
           ++   +C S  C   HSS    D C I+ C L  +  G C     PCP F Y YG  G +
Sbjct: 143 SATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYG-DGSL 202

Query: 210 TGSLTRDVLFTHGNYNNNNNNNKQIPRFCFGCVGATYREPIGIAGFGRGLLSLPFQLGF- 269
              L  D L         +  +  +  F FGC   T  EPIG+AGFGRG LSLP QL   
Sbjct: 203 VAKLYSDSL---------SLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVH 262

Query: 270 -SHKG--FSHCFLPFKF-SNNPNFSSPLILGNLA------------------ISSKDENL 329
             H G  FS+C +   F S+     SPLILG                        K    
Sbjct: 263 SPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEF 322

Query: 330 QFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTH 389
            FT +L++P +P +Y + L+ I+IG  +          LR ID  G GG+++DSGTT+T 
Sbjct: 323 VFTEMLENPKHPYFYSVSLQGISIGKRN----IPAPAMLRRIDKNGGGGVVVDSGTTFTM 382

Query: 390 LPEPLYSQLISNLELVIG--YPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSITFH 449
           LP   Y+ ++   +  +G  + RA +VE ++G   CY +             ++P++  H
Sbjct: 383 LPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN---------QTVKVPALVLH 442

Query: 450 FL-NNVSVVLPQGNNFYAMA----APINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 504
           F  N  SV LP+ N FY              + CL+  +    G D      G   I G+
Sbjct: 443 FAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMN----GGDESELRGGTGAILGN 494

[Close]

BLAST of CsaV3_1G046460 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 4.1e-34
Identity = 129/442 (29.19%), Postives = 181/442 (40.95%), Query Frame = 0


Query: 64  KKRMKAMDQTDGDDNVIEPLREIRDG-YLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLS 123
           ++RM++++      + IE      DG YLM+++IGTP       MDTGSDL W  C    
Sbjct: 69  ERRMRSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCE--- 128

Query: 124 FDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLA 183
             C  C      I       F P  SS+     C S +C D+     P + C    C   
Sbjct: 129 -PCTQCFSQPTPI-------FNPQDSSSFSTLPCESQYCQDL-----PSETCNNNEC--- 188

Query: 184 SLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNYNNNNNNNKQIPRFCFGC---- 243
                        + Y YG      G +  +  FT             +P   FGC    
Sbjct: 189 ------------QYTYGYGDGSTTQGYMATET-FTF--------ETSSVPNIAFGCGEDN 248

Query: 244 VGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISS 303
            G       G+ G G G LSLP QLG     FS+C   +  S+     S L LG+ A S 
Sbjct: 249 QGFGQGNGAGLIGMGWGPLSLPSQLGVGQ--FSYCMTSYGSSS----PSTLALGS-AASG 308

Query: 304 KDENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSG 363
             E    T L+ S + P YYYI L+ IT+G GDN    G+     ++   G GGM+IDSG
Sbjct: 309 VPEGSPSTTLIHSSLNPTYYYITLQGITVG-GDN---LGIPSSTFQLQDDGTGGMIIDSG 368

Query: 364 TTYTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSI 423
           TT T+LP+  Y+ +       I  P     E ++G   C++ P   +        Q+P I
Sbjct: 369 TTLTYLPQDAYNAVAQAFTDQINLPTVD--ESSSGLSTCFQQPSDGST------VQVPEI 428

Query: 424 TFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQ 483
           +  F   V  +  Q      + +P    +  CL   S   +G            IFG+ Q
Sbjct: 429 SMQFDGGVLNLGEQN----ILISPAEGVI--CLAMGSSSQLG----------ISIFGNIQ 435

Query: 484 QQNIEVVYDLEKERLGFQPMDC 501
           QQ  +V+YDL+   + F P  C
Sbjct: 489 QQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of CsaV3_1G046460 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.7e-32
Identity = 119/425 (28.00%), Postives = 180/425 (42.35%), Query Frame = 0


Query: 78  NVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNISG 137
           +V+  L +    Y   L +GTP + V + +DTGSD+ W+ C      C+ C    + I  
Sbjct: 130 SVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCA----PCRRCYSQSDPIFD 189

Query: 138 PRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLASLVKGTCPRPCPSFA 197
           PR        S T     C S  C  + S          AGC+     + TC      + 
Sbjct: 190 PR-------KSKTYATIPCSSPHCRRLDS----------AGCNTR---RKTC-----LYQ 249

Query: 198 YTYGASGVVTGSLTRDVLFTHGNYNNNNNNNKQIPRFCFGCVGATYREPIGIAGFGRGLL 257
            +YG      G  + + L          N  K +   C       +    G+ G G+G L
Sbjct: 250 VSYGDGSFTVGDFSTETL------TFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKL 309

Query: 258 SLPFQLG--FSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKDENLQFTPLLKSPMYP 317
           S P Q G  F+ K FS+C +    S+ P   S ++ GN A+S      +FTPLL +P   
Sbjct: 310 SFPGQTGHRFNQK-FSYCLVDRSASSKP---SSVVFGNAAVS---RIARFTPLLSNPKLD 369

Query: 318 NYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISN 377
            +YY+GL  I++G        GV+  L ++D  GNGG++IDSGT+ T L  P Y  +   
Sbjct: 370 TFYYVGLLGISVGGTRVP---GVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDA 429

Query: 378 LELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSITFHFLNNVSVVLPQGNN 437
               +G    K+    + FD C+ +       S +++ ++P++  HF     V LP  N 
Sbjct: 430 FR--VGAKTLKRAPDFSLFDTCFDL-------SNMNEVKVPTVVLHF-RGADVSLPATN- 484

Query: 438 FYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQNIEVVYDLEKERLGF 497
                 P+++    C  +           +   G   I G+ QQQ   VVYDL   R+GF
Sbjct: 490 ---YLIPVDTNGKFCFAF-----------AGTMGGLSIIGNIQQQGFRVVYDLASSRVGF 484

Query: 498 QPMDC 501
            P  C
Sbjct: 550 APGGC 484

[Close]

BLAST of CsaV3_1G046460 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 3.3e-31
Identity = 127/418 (30.38%), Postives = 171/418 (40.91%), Query Frame = 0


Query: 90  YLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNISGPRLAAFLPTHSS 149
           YLM+LSIGTP Q     MDTGSDL W         CQ C +  N  +      F P  SS
Sbjct: 95  YLMNLSIGTPAQPFSAIMDTGSDLIWT-------QCQPCTQCFNQST----PIFNPQGSS 154

Query: 150 TSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLASLVKGTCPRPCPSFAYTYGASGVVTGS 209
           +     C S  C  + S                     TC      + Y YG      GS
Sbjct: 155 SFSTLPCSSQLCQALSSP--------------------TCSNNFCQYTYGYGDGSETQGS 214

Query: 210 LTRDVLFTHGNYNNNNNNNKQIPRFCFGC----VGATYREPIGIAGFGRGLLSLPFQLGF 269
           +  + L T G+ +        IP   FGC     G       G+ G GRG LSLP QL  
Sbjct: 215 MGTETL-TFGSVS--------IPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDV 274

Query: 270 SHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKDENLQFTPLLKSPMYPNYYYIGLESI 329
           +   FS+C  P   S   N    L+LG+LA +S       T L++S   P +YYI L  +
Sbjct: 275 TK--FSYCMTPIGSSTPSN----LLLGSLA-NSVTAGSPNTTLIQSSQIPTFYYITLNGL 334

Query: 330 TIGNGDNNFRFGV---SFKLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLELVIGY 389
           ++G    + R  +   +F L      G GG++IDSGTT T+     Y  +       I  
Sbjct: 335 SVG----STRLPIDPSAFALN--SNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINL 394

Query: 390 PRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSITFHFLNNVSVVLPQGNNFYAMAAP 449
           P       ++GFDLC++ P   +N       Q+P+   HF +   + LP  N F    +P
Sbjct: 395 PVVN--GSSSGFDLCFQTPSDPSN------LQIPTFVMHF-DGGDLELPSENYF---ISP 434

Query: 450 INSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQNIEVVYDLEKERLGFQPMDC 501
            N  +  CL   S            +    IFG+ QQQN+ VVYD     + F    C
Sbjct: 455 SNGLI--CLAMGS-----------SSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of CsaV3_1G046460 vs. ExPASy Swiss-Prot
Match: O04496 (Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 6.8e-29
Identity = 111/425 (26.12%), Postives = 180/425 (42.35%), Query Frame = 0


Query: 90  YLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNISGPRLAAFLPTHSS 149
           Y++   +GTPPQ++ + +DT +D  W+PC      C  C                 +++S
Sbjct: 104 YVVRAKLGTPPQLMFMVLDTSNDAVWLPCSG----CSGC-----------------SNAS 163

Query: 150 TSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLASLVKGTCPRPCP-----SFAYTYGASG 209
           TS      S++             C+ A C+ A  +  TCP   P     SF  +YG   
Sbjct: 164 TSFNTNSSSTYST---------VSCSTAQCTQARGL--TCPSSSPQPSVCSFNQSYGGDS 223

Query: 210 VVTGSLTRDVLFTHGNYNNNNNNNKQIPRFCFGCVGATYRE---PIGIAGFGRGLLSLPF 269
             + SL +D L    +          IP F FGC+ +       P G+ G GRG +SL  
Sbjct: 224 SFSASLVQDTLTLAPDV---------IPNFSFGCINSASGNSLPPQGLMGLGRGPMSLVS 283

Query: 270 QLGFSHKG-FSHCFLPFKFSNNPNFSSPLILGNLAISSKDENLQFTPLLKSPMYPNYYYI 329
           Q    + G FS+C   F+   +  FS  L LG L    + +++++TPLL++P  P+ YY+
Sbjct: 284 QTTSLYSGVFSYCLPSFR---SFYFSGSLKLGLL---GQPKSIRYTPLLRNPRRPSLYYV 343

Query: 330 GLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLELVI 389
            L  +++G    + +  V       D     G +IDSGT  T   +P+Y  +        
Sbjct: 344 NLTGVSVG----SVQVPVDPVYLTFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFR--- 403

Query: 390 GYPRAKQVELNT-----GFDLCYKVPCKNNNSSFVDDAQLPSITFHFLNNVSVVLPQGNN 449
                KQV +++      FD C+    +N           P IT H + ++ + LP  N 
Sbjct: 404 -----KQVNVSSFSTLGAFDTCFSADNEN---------VAPKITLH-MTSLDLKLPMENT 448

Query: 450 FYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQNIEVVYDLEKERLGF 501
               +A      + CL   SM G+      + N    +  + QQQN+ +++D+   R+G 
Sbjct: 464 LIHSSA----GTLTCL---SMAGI----RQNANAVLNVIANLQQQNLRILFDVPNSRIGI 448

[Close]

BLAST of CsaV3_1G046460 vs. ExPASy TrEMBL
Match: A0A0A0LYP0 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G704590 PE=3 SV=1)

HSP 1 Score: 1067.4 bits (2759), Expect = 1.9e-308
Identity = 518/518 (100.00%), Postives = 518/518 (100.00%), Query Frame = 0


Query: 1   MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN
Sbjct: 1   MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120
           FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNYNNNNNNNKQIPRFCFGCVG 240
           LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNYNNNNNNNKQIPRFCFGCVG
Sbjct: 181 LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNYNNNNNNNKQIPRFCFGCVG 240

Query: 241 ATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKD 300
           ATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKD
Sbjct: 241 ATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKD 300

Query: 301 ENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTT 360
           ENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTT
Sbjct: 301 ENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTT 360

Query: 361 YTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSITF 420
           YTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSITF
Sbjct: 361 YTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSITF 420

Query: 421 HFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQ 480
           HFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQ
Sbjct: 421 HFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQ 480

Query: 481 NIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNVRRNES 519
           NIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNVRRNES
Sbjct: 481 NIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNVRRNES 518

[Close]

BLAST of CsaV3_1G046460 vs. ExPASy TrEMBL
Match: A0A5A7TNC9 (Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G00970 PE=3 SV=1)

HSP 1 Score: 1019.2 bits (2634), Expect = 6.0e-294
Identity = 495/520 (95.19%), Postives = 505/520 (97.12%), Query Frame = 0


Query: 1   MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           MPSISS S ATKFLSLFLLLVH S QTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN
Sbjct: 1   MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120
           FISKKRMKAMDQ DGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNNISGP+LAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNY----NNNNNNNKQIPRFCF 240
           LA+LVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLF HGNY    NNN+NNNKQ+PRFCF
Sbjct: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF 240

Query: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAI 300
           GCVGATYREPIGIAGFGRGLLSLPFQLGFS KGFSHCFLPFKFSNNPNFSSPLILG+LAI
Sbjct: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI 300

Query: 301 SSKDENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLID 360
           SSKDENLQFTPLLKSP+YPNYYYIGLESITIGNG+NNFRFGVSFKLREIDTKGNGGMLID
Sbjct: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360

Query: 361 SGTTYTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLP 420
           SGTTYTHLPEPLYSQLISNLE VI YPRAKQVELNTGFDLCYKVPCKNNNSSFVDD+QLP
Sbjct: 361 SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420

Query: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480
           SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS
Sbjct: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480

Query: 481 FQQQNIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNVRRN 517
           FQQQN++VVYDLEKERLGFQ MDCVSVAA QGLHKNVRRN
Sbjct: 481 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNVRRN 520

[Close]

BLAST of CsaV3_1G046460 vs. ExPASy TrEMBL
Match: A0A1S3CAK9 (aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103498305 PE=3 SV=1)

HSP 1 Score: 1019.2 bits (2634), Expect = 6.0e-294
Identity = 495/520 (95.19%), Postives = 505/520 (97.12%), Query Frame = 0


Query: 1   MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           MPSISS S ATKFLSLFLLLVH S QTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN
Sbjct: 1   MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120
           FISKKRMKAMDQ DGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNNISGP+LAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNY----NNNNNNNKQIPRFCF 240
           LA+LVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLF HGNY    NNN+NNNKQ+PRFCF
Sbjct: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF 240

Query: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAI 300
           GCVGATYREPIGIAGFGRGLLSLPFQLGFS KGFSHCFLPFKFSNNPNFSSPLILG+LAI
Sbjct: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI 300

Query: 301 SSKDENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLID 360
           SSKDENLQFTPLLKSP+YPNYYYIGLESITIGNG+NNFRFGVSFKLREIDTKGNGGMLID
Sbjct: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360

Query: 361 SGTTYTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLP 420
           SGTTYTHLPEPLYSQLISNLE VI YPRAKQVELNTGFDLCYKVPCKNNNSSFVDD+QLP
Sbjct: 361 SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420

Query: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480
           SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS
Sbjct: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480

Query: 481 FQQQNIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNVRRN 517
           FQQQN++VVYDLEKERLGFQ MDCVSVAA QGLHKNVRRN
Sbjct: 481 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNVRRN 520

[Close]

BLAST of CsaV3_1G046460 vs. ExPASy TrEMBL
Match: A0A6J1CMP8 (probable aspartyl protease At4g16563 OS=Momordica charantia OX=3673 GN=LOC111012684 PE=3 SV=1)

HSP 1 Score: 827.4 bits (2136), Expect = 3.3e-236
Identity = 418/529 (79.02%), Postives = 458/529 (86.58%), Query Frame = 0


Query: 1   MPSISSIST--ATKFLSLFLLLVHV--STQTLATNPKTNFPK-DSLVLGLVHSRTSLLTP 60
           MP+  S +T  ++K L+ FLLL+ +   ++T A   + NFP  DSLVLGLVHSRTSLLTP
Sbjct: 1   MPTTKSSATTISSKVLTFFLLLILLLSVSETAARPHRNNFPNTDSLVLGLVHSRTSLLTP 60

Query: 61  KKGY---NFISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSD 120
           K+GY      S    K M++  G DNVIEPLREIRDGYL+SL++GTPPQV+QVYMDTGSD
Sbjct: 61  KRGYFSRKGSSSSINKPMEEI-GSDNVIEPLREIRDGYLISLTLGTPPQVIQVYMDTGSD 120

Query: 121 LTWVPCGNLSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFD 180
           LTWVPCGNLSFDCQDCEEYQNN+SGP+LA F PTHSSTSIRDTCGSSFCMDIHSSDNPFD
Sbjct: 121 LTWVPCGNLSFDCQDCEEYQNNVSGPKLAPFSPTHSSTSIRDTCGSSFCMDIHSSDNPFD 180

Query: 181 PCTIAGCSLASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNYNNNNNNNKQIP 240
           PCTIAGCSLA+LVKGTCPRPCPSFAYTYGASGVVTG+LT+DV+  HG    + N+  QIP
Sbjct: 181 PCTIAGCSLATLVKGTCPRPCPSFAYTYGASGVVTGTLTKDVILMHG---VSPNSTTQIP 240

Query: 241 RFCFGCVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILG 300
           RFCFGCVGATYREPIGIAGFGRGLLSLP QLGFSHKGFSHCFLPFKFSNNPNFSSPLILG
Sbjct: 241 RFCFGCVGATYREPIGIAGFGRGLLSLPSQLGFSHKGFSHCFLPFKFSNNPNFSSPLILG 300

Query: 301 NLAISSKDENLQFTPLLKSPMYPNYYYIGLESITIGN--GDNNFRFGVSFKLREIDTKGN 360
           +LAISSKD +LQFTPLLKSP+YPNYYYIGLES+TIG+  G+NN RFGVS KLREIDTKGN
Sbjct: 301 SLAISSKDHSLQFTPLLKSPLYPNYYYIGLESVTIGDGIGNNNSRFGVSLKLREIDTKGN 360

Query: 361 GGMLIDSGTTYTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNN--SS 420
           GGMLIDSGTTYTHLPEPLYSQLISNLE V+ YPRAKQVE+NTGFDLCYK+PCKNN   SS
Sbjct: 361 GGMLIDSGTTYTHLPEPLYSQLISNLESVVTYPRAKQVEINTGFDLCYKIPCKNNTNFSS 420

Query: 421 FVDD---AQLPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDND- 480
            +DD     LPSITFHFLNNVSVVLPQGNNFYAMAAP NSTVVKCLL+QSMDG G D D 
Sbjct: 421 SMDDYCSNLLPSITFHFLNNVSVVLPQGNNFYAMAAPTNSTVVKCLLFQSMDGGGGDGDG 480

Query: 481 -SDDNGPAGIFGSFQQQNIEVVYDLEKERLGFQPMDCVSVAAKQGLHKN 513
             DD+GPAGIFGSFQQQN+EVVYDL+KER+GFQ MDC S AA QGLHKN
Sbjct: 481 NGDDDGPAGIFGSFQQQNMEVVYDLQKERIGFQTMDCASSAASQGLHKN 525

[Close]

BLAST of CsaV3_1G046460 vs. ExPASy TrEMBL
Match: A0A6J1EHM1 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111434252 PE=3 SV=1)

HSP 1 Score: 824.3 bits (2128), Expect = 2.8e-235
Identity = 409/511 (80.04%), Postives = 451/511 (88.26%), Query Frame = 0


Query: 1   MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           M SI++ S     L L L+      QTLA NPKT F KDSLVLGLVHSRTSLLTPK+GYN
Sbjct: 1   MASIAARSYFVLVLVLVLVSGEAMGQTLA-NPKTKFLKDSLVLGLVHSRTSLLTPKRGYN 60

Query: 61  FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120
            +  KR+K M+   G+D+VIEPLREIRDGYLMSL++GTPPQV+QVYMDTGSDLTWVPCGN
Sbjct: 61  SLLTKRIKPMEM--GNDDVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDC+EYQNN+ GP+LAAFLPTHSSTSIRDTCGSSFC+DIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCDEYQNNVLGPKLAAFLPTHSSTSIRDTCGSSFCIDIHSSDNPFDPCTIAGCS 180

Query: 181 LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNYNNNNNNNKQIPRFCFGCVG 240
           LA+LVKGTCPRPCPSF+YTYGASG+V G+LT+DV+F HG   N+ N++++IP+FCFGCVG
Sbjct: 181 LATLVKGTCPRPCPSFSYTYGASGLVIGTLTKDVIFIHG---NSPNSSRKIPKFCFGCVG 240

Query: 241 ATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKD 300
           ATYREPIGIAGFGRGLLSLP QLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK 
Sbjct: 241 ATYREPIGIAGFGRGLLSLPSQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK- 300

Query: 301 ENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTT 360
           E+L+FTP LKSP YPNYYYIGLESITIGNG+N  RFGVS +LREIDTKGNGG+LIDSGTT
Sbjct: 301 EHLKFTPFLKSPFYPNYYYIGLESITIGNGENYSRFGVSLQLREIDTKGNGGILIDSGTT 360

Query: 361 YTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSITF 420
           YTHLPEPLYSQLISNLE +I YPRAK+ ELNTGFDLCYKVP K NN+ F D+ +LPSITF
Sbjct: 361 YTHLPEPLYSQLISNLESLISYPRAKEHELNTGFDLCYKVPYK-NNTFFSDEFELPSITF 420

Query: 421 HFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQ 480
           HFLNNVSVVLPQGN+FYAMAAP NSTVVKCLL+QSMDG GD       GPAGIFGSFQQQ
Sbjct: 421 HFLNNVSVVLPQGNSFYAMAAPSNSTVVKCLLFQSMDGDGD-------GPAGIFGSFQQQ 480

Query: 481 NIEVVYDLEKERLGFQPMDCVSVAAKQGLHK 512
           N+EVVYDLEKERLGF+ MDC SVA  QGLHK
Sbjct: 481 NLEVVYDLEKERLGFEAMDCASVAVSQGLHK 496

[Close]

BLAST of CsaV3_1G046460 vs. TAIR 10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 595.9 bits (1535), Expect = 3.1e-170
Identity = 308/515 (59.81%), Postives = 375/515 (72.82%), Query Frame = 0


Query: 7   ISTATKFLSLFL---LLVHVSTQTLATNPKTNFPKDS--LVLGLVHSRTSLLTPKKGYNF 66
           + T T  L LFL   LL++ + +T A   K      S  LVL L  S  SL TPK     
Sbjct: 1   METQTHVLFLFLLITLLLNTTNKTQARQHKNPSSSSSSFLVLTLTKSSVSLPTPKSQTQE 60

Query: 67  ISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNL 126
             KK + ++D       V+EPLRE+RDGYL++L+IGTPPQ VQVY+DTGSDLTWVPCGNL
Sbjct: 61  RIKKPLSSVDV------VMEPLREVRDGYLITLNIGTPPQAVQVYLDTGSDLTWVPCGNL 120

Query: 127 SFDCQDCEEYQNN-ISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 186
           SFDC +C + +NN +  P  + F P HSSTS RD+C SSFC++IHSSDNPFDPC +AGCS
Sbjct: 121 SFDCIECYDLKNNDLKSP--SVFSPLHSSTSFRDSCASSFCVEIHSSDNPFDPCAVAGCS 180

Query: 187 LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNYNNNNNNNKQIPRFCFGCVG 246
           ++ L+K TC RPCPSFAYTYG  G+++G LTRD+L             + +PRF FGCV 
Sbjct: 181 VSMLLKSTCVRPCPSFAYTYGEGGLISGILTRDIL---------KARTRDVPRFSFGCVT 240

Query: 247 ATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAIS-SK 306
           +TYREPIGIAGFGRGLLSLP QLGF  KGFSHCFLPFKF NNPN SSPLILG  A+S + 
Sbjct: 241 STYREPIGIAGFGRGLLSLPSQLGFLEKGFSHCFLPFKFVNNPNISSPLILGASALSINL 300

Query: 307 DENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGT 366
            ++LQFTP+L +PMYPN YYIGLESITIG   N     V   LR+ D++GNGGML+DSGT
Sbjct: 301 TDSLQFTPMLNTPMYPNSYYIGLESITIGT--NITPTQVPLTLRQFDSQGNGGMLVDSGT 360

Query: 367 TYTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNN-SSFVDDAQL--P 426
           TYTHLPEP YSQL++ L+  I YPRA + E  TGFDLCYKVPC NNN +S  +D  +  P
Sbjct: 361 TYTHLPEPFYSQLLTTLQSTITYPRATETESRTGFDLCYKVPCPNNNLTSLENDVMMIFP 420

Query: 427 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 486
           SITFHFLNN +++LPQGN+FYAM+AP + +VV+CLL+Q+M       +  D GPAG+FGS
Sbjct: 421 SITFHFLNNATLLLPQGNSFYAMSAPSDGSVVQCLLFQNM-------EDGDYGPAGVFGS 480

Query: 487 FQQQNIEVVYDLEKERLGFQPMDCVSVAAKQGLHK 512
           FQQQN++VVYDLEKER+GFQ MDCV  AA  GL++
Sbjct: 481 FQQQNVKVVYDLEKERIGFQAMDCVLEAASHGLNQ 489

[Close]

BLAST of CsaV3_1G046460 vs. TAIR 10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 211.5 bits (537), Expect = 1.7e-54
Identity = 150/447 (33.56%), Postives = 204/447 (45.64%), Query Frame = 0


Query: 90  YLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNISGPRLAAFLPTHSS 149
           YL+SLS+G+    V +Y+DTGSDL W PC    F C  CE      S P       + SS
Sbjct: 83  YLISLSVGSSSSAVSLYLDTGSDLVWFPC--RPFTCILCESKPLPPSPP------SSLSS 142

Query: 150 TSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLASLVKGTC---PRPCPSFAYTYGASGVV 209
           ++   +C S  C   HSS    D C I+ C L  +  G C     PCP F Y YG  G +
Sbjct: 143 SATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYG-DGSL 202

Query: 210 TGSLTRDVLFTHGNYNNNNNNNKQIPRFCFGCVGATYREPIGIAGFGRGLLSLPFQLGF- 269
              L  D L         +  +  +  F FGC   T  EPIG+AGFGRG LSLP QL   
Sbjct: 203 VAKLYSDSL---------SLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVH 262

Query: 270 -SHKG--FSHCFLPFKF-SNNPNFSSPLILGNLA------------------ISSKDENL 329
             H G  FS+C +   F S+     SPLILG                        K    
Sbjct: 263 SPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEF 322

Query: 330 QFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTH 389
            FT +L++P +P +Y + L+ I+IG  +          LR ID  G GG+++DSGTT+T 
Sbjct: 323 VFTEMLENPKHPYFYSVSLQGISIGKRN----IPAPAMLRRIDKNGGGGVVVDSGTTFTM 382

Query: 390 LPEPLYSQLISNLELVIG--YPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSITFH 449
           LP   Y+ ++   +  +G  + RA +VE ++G   CY +             ++P++  H
Sbjct: 383 LPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN---------QTVKVPALVLH 442

Query: 450 FL-NNVSVVLPQGNNFYAMA----APINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 504
           F  N  SV LP+ N FY              + CL+  +    G D      G   I G+
Sbjct: 443 FAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMN----GGDESELRGGTGAILGN 494

[Close]

BLAST of CsaV3_1G046460 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 180.3 bits (456), Expect = 4.1e-45
Identity = 133/421 (31.59%), Postives = 189/421 (44.89%), Query Frame = 0


Query: 89  GYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNISGPRLAAFLPTHS 148
           GY +SLS GTP Q +    DTGS L W+PC +  + C  C+   + +    +  F+P +S
Sbjct: 89  GYSVSLSFGTPSQTIPFVFDTGSSLVWLPCTS-RYLCSGCD--FSGLDPTLIPRFIPKNS 148

Query: 149 STSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLASLVKGTCPRPCPSFAYTYGASGVVTG 208
           S+S    C S  C  ++    P   C   GC   +     C   CP +   YG       
Sbjct: 149 SSSKIIGCQSPKCQFLY---GPNVQC--RGCDPNT---RNCTVGCPPYILQYGLGSTAGV 208

Query: 209 SLTRDVLFTHGNYNNNNNNNKQIPRFCFGCVGATYREPIGIAGFGRGLLSLPFQLGFSHK 268
            +T  + F           +  +P F  GC   + R+P GIAGFGRG +SLP Q+    K
Sbjct: 209 LITEKLDFP----------DLTVPDFVVGCSIISTRQPAGIAGFGRGPVSLPSQMNL--K 268

Query: 269 GFSHCFLPFKFSNNPNFSSPLILGNLA---ISSKDENLQFTPLLKSPMYPN-----YYYI 328
            FSHC +  +F ++ N ++ L L   +     SK   L +TP  K+P   N     YYY+
Sbjct: 269 RFSHCLVSRRF-DDTNVTTDLDLDTGSGHNSGSKTPGLTYTPFRKNPNVSNKAFLEYYYL 328

Query: 329 GLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNL-ELV 388
            L  I +G         + +K     T G+GG ++DSG+T+T +  P++  +       +
Sbjct: 329 NLRRIYVGRK----HVKIPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAEEFASQM 388

Query: 389 IGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSITFHFLNNVSVVLPQGNNFYAM 448
             Y R K +E  TG   C+ +  K        D  +P + F F     + LP  N F   
Sbjct: 389 SNYTREKDLEKETGLGPCFNISGKG-------DVTVPELIFEFKGGAKLELPLSNYF--- 448

Query: 449 AAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQNIEVVYDLEKERLGFQPMD 501
              + +T   CL   S   V   N S   GPA I GSFQQQN  V YDLE +R GF    
Sbjct: 449 -TFVGNTDTVCLTVVSDKTV---NPSGGTGPAIILGSFQQQNYLVEYDLENDRFGFAKKK 467

[Close]

BLAST of CsaV3_1G046460 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 152.5 bits (384), Expect = 9.2e-37
Identity = 156/516 (30.23%), Postives = 218/516 (42.25%), Query Frame = 0


Query: 13  FLSLFLLLVHVSTQTLATNPKT---NFPKDSLVLGLVH-----SRTSLLTPKKGYN--FI 72
           FL LF  L+ VS+   +   +T   N P+    L L H     + T +   ++G N  F 
Sbjct: 14  FLILFSCLISVSSSRRSLIDRTLPKNLPRSGFRLSLRHVDSGKNLTKIQKIQRGINRGFH 73

Query: 73  SKKRMKAM------DQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWV 132
              R+ A+       + D  +N+  P       +LM LSIG P       +DTGSDL W 
Sbjct: 74  RLNRLGAVAVLAVASKPDDTNNIKAPTHGGSGEFLMELSIGNPAVKYSAIVDTGSDLIWT 133

Query: 133 PCGNLSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTI 192
            C      C +C +    I       F P  SS+  +  C S  C  +  S+   D    
Sbjct: 134 QCK----PCTECFDQPTPI-------FDPEKSSSYSKVGCSSGLCNALPRSNCNED---- 193

Query: 193 AGCSLASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNYNNNNNNNKQIPRFCF 252
                    K  C      + YTYG      G L  +  FT  + N+       I    F
Sbjct: 194 ---------KDAC-----EYLYTYGDYSSTRGLLATET-FTFEDENS-------ISGIGF 253

Query: 253 GC----VGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILG 312
           GC     G  + +  G+ G GRG LSL  QL      FS+C    + S     SS L +G
Sbjct: 254 GCGVENEGDGFSQGSGLVGLGRGPLSLISQL--KETKFSYCLTSIEDS---EASSSLFIG 313

Query: 313 NLA--------ISSKDENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLRE 372
           +LA         S   E  +   LL++P  P++YY+ L+ IT+G      R  V     E
Sbjct: 314 SLASGIVNKTGASLDGEVTKTMSLLRNPDQPSFYYLELQGITVGAK----RLSVEKSTFE 373

Query: 373 IDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKN 432
           +   G GGM+IDSGTT T+L E  +  L       +  P       +TG DLC+K+P   
Sbjct: 374 LAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLP--VDDSGSTGLDLCFKLPDAA 433

Query: 433 NNSSFVDDAQLPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDND 492
            N +      +P + FHF     + LP G N+  M A  +ST V CL   S +G+     
Sbjct: 434 KNIA------VPKMIFHF-KGADLELP-GENY--MVAD-SSTGVLCLAMGSSNGM----- 458

Query: 493 SDDNGPAGIFGSFQQQNIEVVYDLEKERLGFQPMDC 501
                   IFG+ QQQN  V++DLEKE + F P +C
Sbjct: 494 -------SIFGNVQQQNFNVLHDLEKETVSFVPTEC 458

[Close]

BLAST of CsaV3_1G046460 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 149.4 bits (376), Expect = 7.7e-36
Identity = 142/509 (27.90%), Postives = 204/509 (40.08%), Query Frame = 0


Query: 13  FLSLFLL----LVHVSTQT----LATNPKTNFPKDSLVLGLVHSRTSLLT-PKKGYNFIS 72
           FLSLFLL    +  VS       L    K+ FP  +  L L   R   L+  +K   F+ 
Sbjct: 11  FLSLFLLPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLHFLSLRRKPIPFVK 70

Query: 73  KKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSF 132
              +       G              Y + L IG PPQ + +  DTGSDL WV C     
Sbjct: 71  SPVVSGAASGSGQ-------------YFVDLRIGQPPQSLLLIADTGSDLVWVKCS---- 130

Query: 133 DCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLAS 192
            C++C  +           F P HSST     C    C  +   D        A     +
Sbjct: 131 ACRNCSHHS------PATVFFPRHSSTFSPAHCYDPVCRLVPKPDR-------APICNHT 190

Query: 193 LVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNYNNNNNNNKQIPRFCFGC----- 252
            +  TC      + Y Y    + +G   R+      +   ++    ++    FGC     
Sbjct: 191 RIHSTC-----HYEYGYADGSLTSGLFARETT----SLKTSSGKEARLKSVAFGCGFRIS 250

Query: 253 ----VGATYREPIGIAGFGRGLLSLPFQLG--FSHKGFSHCFLPFKFSNNPNFSSPLILG 312
                G ++    G+ G GRG +S   QLG  F +K FS+C + +  S  P  +S LI+G
Sbjct: 251 GQSVSGTSFNGANGVMGLGRGPISFASQLGRRFGNK-FSYCLMDYTLSPPP--TSYLIIG 310

Query: 313 NLAISSKDENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGG 372
           N         L FTPLL +P+ P +YY+ L+S+ +    N  +  +   + EID  GNGG
Sbjct: 311 N--GGDGISKLFFTPLLTNPLSPTFYYVKLKSVFV----NGAKLRIDPSIWEIDDSGNGG 370

Query: 373 MLIDSGTTYTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDD 432
            ++DSGTT   L EP Y  +I+ +   +  P A    L  GFDLC  V     +     +
Sbjct: 371 TVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIAD--ALTPGFDLCVNV-----SGVTKPE 430

Query: 433 AQLPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMD-GVGDDNDSDDNGPA 492
             LP + F F      V P  N F           ++CL  QS+D  VG           
Sbjct: 431 KILPRLKFEFSGGAVFVPPPRNYFIE-----TEEQIQCLAIQSVDPKVG----------F 449

Query: 493 GIFGSFQQQNIEVVYDLEKERLGFQPMDC 501
            + G+  QQ     +D ++ RLGF    C
Sbjct: 491 SVIGNLMQQGFLFEFDRDRSRLGFSRRGC 449

The following BLAST results are available for this feature:

BLAST of CsaV3_1G046460 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145478.2	3.9e-308	100.00	probable aspartyl protease At4g16563 [Cucumis sativus] >KGN66888.1 hypothetical ...	[more]
XP_008459091.1	1.2e-293	95.19	PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis melo] >KAA0043307.1 aspart...	[more]
XP_038893627.1	6.4e-266	87.74	probable aspartyl protease At4g16563 [Benincasa hispida]	[more]
XP_023520027.1	2.1e-237	81.42	probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo]	[more]
KAG6583807.1	4.0e-236	80.31	putative aspartyl protease, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CsaV3_1G046460 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q940R4	2.3e-53	33.56	Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656...	[more]
Q766C2	4.1e-34	29.19	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q9LNJ3	1.7e-32	28.00	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q766C3	3.3e-31	30.38	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
O04496	6.8e-29	26.12	Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1	[more]

BLAST of CsaV3_1G046460 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LYP0	1.9e-308	100.00	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G70459...	[more]
A0A5A7TNC9	6.0e-294	95.19	Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3CAK9	6.0e-294	95.19	aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103498305 PE=3 S...	[more]
A0A6J1CMP8	3.3e-236	79.02	probable aspartyl protease At4g16563 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A6J1EHM1	2.8e-235	80.04	probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114342...	[more]

BLAST of CsaV3_1G046460 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G45120.1	3.1e-170	59.81	Eukaryotic aspartyl protease family protein	[more]
AT4G16563.1	1.7e-54	33.56	Eukaryotic aspartyl protease family protein	[more]
AT3G52500.1	4.1e-45	31.59	Eukaryotic aspartyl protease family protein	[more]
AT2G03200.1	9.2e-37	30.23	Eukaryotic aspartyl protease family protein	[more]
AT3G25700.1	7.7e-36	27.90	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 318..496 e-value: 1.9E-25 score: 89.5
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 84..292 e-value: 1.5E-30 score: 108.6
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 293..506 e-value: 1.6E-44 score: 153.7
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 89..502
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 90..293 e-value: 1.9E-28 score: 99.9
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 50..505
None	No IPR available	PANTHER	PTHR47967:SF47	CHLOROPLAST NUCLEOID DNA-BINDING PROTEIN-LIKE	coord: 50..505
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 353..364
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 90..496 score: 32.846729
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 89..500 e-value: 3.19115E-70 score: 223.679

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_1G046460.1	CsaV3_1G046460.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity