CsaV3_1G042190 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_1G042190 vs. NCBI nr
Match: XP_011660094.1 (L-ascorbate oxidase-like isoform X1 [Cucumis sativus] >KAE8653486.1 hypothetical protein Csa_007106 [Cucumis sativus])

HSP 1 Score: 1384.8 bits (3583), Expect = 0.0e+00
Identity = 662/662 (100.00%), Postives = 662/662 (100.00%), Query Frame = 0

Query: 1   MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIV 60
           MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIV
Sbjct: 1   MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIV 60

Query: 61  MGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPIN 120
           MGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPIN
Sbjct: 61  MGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPIN 120

Query: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180
           PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW
Sbjct: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180

Query: 181 WHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCEWKDQCAPFVL 240
           WHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCEWKDQCAPFVL
Sbjct: 181 WHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCEWKDQCAPFVL 240

Query: 241 KVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLV 300
           KVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLV
Sbjct: 241 KVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLV 300

Query: 301 TTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNF 360
           TTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNF
Sbjct: 301 TTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNF 360

Query: 361 TYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGL 420
           TYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGL
Sbjct: 361 TYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGL 420

Query: 421 NSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEI 480
           NSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEI
Sbjct: 421 NSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEI 480

Query: 481 HPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540
           HPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI
Sbjct: 481 HPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540

Query: 541 FHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPRPNPPGLDNPYYI 600
           FHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPRPNPPGLDNPYYI
Sbjct: 541 FHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPRPNPPGLDNPYYI 600

Query: 601 IWIGLVIFLCVIGLCYVCLYRIRFNNLSWRPYLGISESQSNQIEFSDLSQSNPNPNPTHE 660
           IWIGLVIFLCVIGLCYVCLYRIRFNNLSWRPYLGISESQSNQIEFSDLSQSNPNPNPTHE
Sbjct: 601 IWIGLVIFLCVIGLCYVCLYRIRFNNLSWRPYLGISESQSNQIEFSDLSQSNPNPNPTHE 660

Query: 661 EP 663
           EP
Sbjct: 661 EP 662

BLAST of CsaV3_1G042190 vs. NCBI nr
Match: XP_031736736.1 (L-ascorbate oxidase-like isoform X2 [Cucumis sativus])

HSP 1 Score: 1230.3 bits (3182), Expect = 0.0e+00
Identity = 600/662 (90.63%), Postives = 600/662 (90.63%), Query Frame = 0

Query: 1   MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIV 60
           MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIV
Sbjct: 1   MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIV 60

Query: 61  MGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPIN 120
           MGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPIN
Sbjct: 61  MGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPIN 120

Query: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180
           PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW
Sbjct: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180

Query: 181 WHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCEWKDQCAPFVL 240
           WHASVHEQEVGLKSNPMRWVGEPQ                                    
Sbjct: 181 WHASVHEQEVGLKSNPMRWVGEPQ------------------------------------ 240

Query: 241 KVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLV 300
                                     NHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLV
Sbjct: 241 --------------------------NHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLV 300

Query: 301 TTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNF 360
           TTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNF
Sbjct: 301 TTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNF 360

Query: 361 TYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGL 420
           TYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGL
Sbjct: 361 TYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGL 420

Query: 421 NSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEI 480
           NSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEI
Sbjct: 421 NSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEI 480

Query: 481 HPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540
           HPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI
Sbjct: 481 HPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540

Query: 541 FHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPRPNPPGLDNPYYI 600
           FHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPRPNPPGLDNPYYI
Sbjct: 541 FHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPRPNPPGLDNPYYI 600

Query: 601 IWIGLVIFLCVIGLCYVCLYRIRFNNLSWRPYLGISESQSNQIEFSDLSQSNPNPNPTHE 660
           IWIGLVIFLCVIGLCYVCLYRIRFNNLSWRPYLGISESQSNQIEFSDLSQSNPNPNPTHE
Sbjct: 601 IWIGLVIFLCVIGLCYVCLYRIRFNNLSWRPYLGISESQSNQIEFSDLSQSNPNPNPTHE 600

Query: 661 EP 663
           EP
Sbjct: 661 EP 600

BLAST of CsaV3_1G042190 vs. NCBI nr
Match: XP_008451098.1 (PREDICTED: L-ascorbate oxidase-like [Cucumis melo])

HSP 1 Score: 1229.2 bits (3179), Expect = 0.0e+00
Identity = 599/674 (88.87%), Postives = 625/674 (92.73%), Query Frame = 0

Query: 1   MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIV 60
           MAKVANQP L P+PFLV FFV SI FGFGITLSKAVSQTRYYKFEV+YMFWSPDC+ENIV
Sbjct: 1   MAKVANQP-LFPLPFLV-FFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIV 60

Query: 61  MGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPIN 120
           +GINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRG+PWADGTASISQCPIN
Sbjct: 61  LGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPIN 120

Query: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180
           PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW
Sbjct: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180

Query: 181 WHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCEWKDQCAPFVL 240
           WH S+ EQEVGLKSNPMRW+GE Q ILINGTGQFNCTSL+KYNTSSEHCEWKDQCAPFVL
Sbjct: 181 WHTSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVL 240

Query: 241 KVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLV 300
           KVEPNKTYRIRIASTTSL+ALNFAI+NHTMLVVEADGNYV  F TSDIDIYSGESYSVLV
Sbjct: 241 KVEPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLV 300

Query: 301 TTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNF 360
           TTNQ+TTQNYWASIGVRGR+PKTSPALLLFNYLPNSVAKLPISPPPE PKW+DYDRSK+F
Sbjct: 301 TTNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSF 360

Query: 361 TYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGL 420
           T RVLASAMNTTKPPPIFTRRISLLNTQN +NG F KWAINNISLTLPSTPYLGAIKFGL
Sbjct: 361 TNRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGL 420

Query: 421 NSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEI 480
           NSTFNQT PPETFPKNYNIKIPPPNPNTNTGN VYRFRSGEIVDVILQNANAL+NNTSEI
Sbjct: 421 NSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEI 480

Query: 481 HPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540
           HPWHLHGHDFWVLG+GEGKFS  DETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI
Sbjct: 481 HPWHLHGHDFWVLGFGEGKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540

Query: 541 FHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPRPNP---PGLDNP 600
           FHCHIEPHLDMGMGVIFAEAVE+VREIPPYALACGRTAT  VVPIIP+P P   PG   P
Sbjct: 541 FHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIP 600

Query: 601 YYIIW-IGLVIFLCVIGLCYVCLYRI------RFNNLSWRPYLGISESQSNQIEFSDLSQ 660
           Y+++W IGLVI +C IGL  VCLYRI       FNNLSWRPYLG+S+SQSNQIEFSDLS+
Sbjct: 601 YFMLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQSNQIEFSDLSR 660

Query: 661 SN---PNPNPTHEE 662
           SN      NPT EE
Sbjct: 661 SNLTQEESNPTQEE 672

BLAST of CsaV3_1G042190 vs. NCBI nr
Match: CAA71275.1 (L-ascorbate oxidase [Cucumis melo])

HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 597/674 (88.58%), Postives = 624/674 (92.58%), Query Frame = 0

Query: 1   MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIV 60
           MAKVANQP L P+PFLV FFV SI FGFGITLSKAVSQTRYYKFEV+YMFWSPDC+ENIV
Sbjct: 1   MAKVANQP-LFPLPFLV-FFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIV 60

Query: 61  MGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPIN 120
           +GINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRG+PWADGTASISQCPIN
Sbjct: 61  LGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPIN 120

Query: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180
           PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW
Sbjct: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180

Query: 181 WHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCEWKDQCAPFVL 240
           WH S+ EQEVGLKSNPMRW+GE Q ILINGTGQFNCTSL+KYNTSSEHCEWKDQCAPFVL
Sbjct: 181 WHTSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVL 240

Query: 241 KVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLV 300
           KVEPNKTYRIRIASTTSL+ALNFAI+NHTMLVVEADGNYV  F TSDIDIYSGESYSVLV
Sbjct: 241 KVEPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLV 300

Query: 301 TTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNF 360
           TTNQ+TTQNYWASIGVRGR+PKTSPALLLFNYLPNSVAKLPISPPPE PKW+DYDRSK+F
Sbjct: 301 TTNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSF 360

Query: 361 TYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGL 420
           T RVLASAMNTTKPPPIFTRRISLLNTQN +NG F KWAINNISLTLPSTPYLGAIKFGL
Sbjct: 361 TNRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGL 420

Query: 421 NSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEI 480
           NSTFNQT PPETFPKNYNIKIPPPNPNTNTGN VYRFRSGEIVDVILQNANAL+NNTSEI
Sbjct: 421 NSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEI 480

Query: 481 HPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540
           HPWHLHGHDFWVLG+G+ KFS  DETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI
Sbjct: 481 HPWHLHGHDFWVLGFGDFKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540

Query: 541 FHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPRPNP---PGLDNP 600
           FHCHIEPHLDMGMGVIFAEAVE+VREIPPYALACGRTAT  VVPIIP+P P   PG   P
Sbjct: 541 FHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIP 600

Query: 601 YYIIW-IGLVIFLCVIGLCYVCLYRI------RFNNLSWRPYLGISESQSNQIEFSDLSQ 660
           Y+++W IGLVI +C IGL  VCLYRI       FNNLSWRPYLG+S+SQSNQIEFSDLS+
Sbjct: 601 YFMLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQSNQIEFSDLSR 660

Query: 661 SN---PNPNPTHEE 662
           SN      NPT EE
Sbjct: 661 SNLTQEESNPTQEE 672

BLAST of CsaV3_1G042190 vs. NCBI nr
Match: NP_001284440.1 (L-ascorbate oxidase-like [Cucumis melo] >AAF35910.1 ascorbate oxidase AO1 [Cucumis melo])

HSP 1 Score: 940.3 bits (2429), Expect = 9.3e-270
Identity = 448/579 (77.37%), Postives = 499/579 (86.18%), Query Frame = 0

Query: 3   KVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIVMG 62
           KVANQP L PMPF  IFF+FSIFFGFGITLS+A SQTR++K+EVEYMF SPDC EN+VMG
Sbjct: 4   KVANQP-LFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMG 63

Query: 63  INGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPINPG 122
           IN  FPGP IRANV DTVVVE+IN LSTE VVIHWHGILQ+G+PWADGTASISQC INPG
Sbjct: 64  INHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPG 123

Query: 123 ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH 182
           E+FTY+F VDKPGTYFYHGH GMQRAAGLYGSLIVDLEEGKKEPF YDEEINLLLSDWWH
Sbjct: 124 ESFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWH 183

Query: 183 ASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCE--WKDQCAPFVL 242
            SV +QEVGL S+PMRW+GEPQ+ILING GQ NC+   KY  + E C+    ++CAPF+L
Sbjct: 184 ESVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEECAPFIL 243

Query: 243 KVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLV 302
           KV+PNKTYRIRI STTSL+ALNFAI NH +LVVEADGNYVQ F+TSDIDIYSGESYSVL+
Sbjct: 244 KVKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLI 303

Query: 303 TTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNF 362
            T+Q+ +QNYW S+GVRGREPKT P L L NYLPNS +KLPISPPPE PKWNDYDRSKNF
Sbjct: 304 NTDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNF 363

Query: 363 TYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGL 422
           TYR+LA AM + KPP   TRRI LLNTQN MNG + KWAINN+SLTLPSTPYLGAIK G+
Sbjct: 364 TYRILA-AMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGI 423

Query: 423 NSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEI 482
           N TF+Q  PPET+P+NY+I  PPPN NT TGN VYRF+ GEIVDV+LQNAN L++  SEI
Sbjct: 424 NDTFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEI 483

Query: 483 HPWHLHGHDFWVLGYGEGKFSDE-DETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVW 542
           HPWHLHGHDFWVLGYGEGKFS   DETKLNLK+PPL+NTVVIFPFGWT +RFVADNPGVW
Sbjct: 484 HPWHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVW 543

Query: 543 IFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTA 579
            FHCHIEPHL MGMGV+FAE V+KV  IP +A+ACG TA
Sbjct: 544 AFHCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTA 580

BLAST of CsaV3_1G042190 vs. ExPASy Swiss-Prot
Match: P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 892.1 bits (2304), Expect = 3.8e-258
Identity = 430/591 (72.76%), Postives = 481/591 (81.39%), Query Frame = 0

Query: 1   MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKA-VSQTRYYKFEVEYMFWSPDCIENI 60
           MAKVA++P   P PFL  F V SI FGFGITLS+A   + ++YK++VEYMFWSPDC+ENI
Sbjct: 1   MAKVADKP-FFPKPFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENI 60

Query: 61  VMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPI 120
           VMGING FPGP IRAN  D VVVE+ N L TEGVVIHWHGILQRG+PWADGTASISQC I
Sbjct: 61  VMGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAI 120

Query: 121 NPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSD 180
           NPGE FTY F VDK GTYFYHGH GMQR+AGLYGSLIVD  EG+ EPF YDEEINLLLSD
Sbjct: 121 NPGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSD 180

Query: 181 WWHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCE--WKDQCAP 240
           WWH SVH+QEVGL S PMRW+GEPQ+ILING GQF+C+   KYN   + CE   K++CAP
Sbjct: 181 WWHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAP 240

Query: 241 FVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYSGESYS 300
           F+L V+P KTYRIRIASTT+LA+LNFAI NH +LVVEADGNYVQ FVTSDIDIYSGESYS
Sbjct: 241 FILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYS 300

Query: 301 VLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRS 360
           VL+TT+Q+  +NYW SIGVR R PKT P L L NYLPNS +KLPISPPPE P W D+DRS
Sbjct: 301 VLITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRS 360

Query: 361 KNFTYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIK 420
           KNFT+R+ A AM + KPP  + RR+ LLNTQN +NG F+KWAINN+SL LP TPYL A+K
Sbjct: 361 KNFTFRIFA-AMGSPKPPVRYNRRLFLLNTQNRING-FMKWAINNVSLALPPTPYLAAMK 420

Query: 421 FGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNT 480
             LN+ FNQ  PPETFP NY+I  PPPNP T TGN VY+F  GE VDVILQNAN L+ N 
Sbjct: 421 MRLNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNM 480

Query: 481 SEIHPWHLHGHDFWVLGYGEGKF-SDEDETKLNLKNPPLKNTVVIFPFGWTAIRFVADNP 540
           SEIHPWHLHGHDFWVLGYGEGKF + EDE KLNLKNPPL+NTVVIFP+GWTAIRFVADNP
Sbjct: 481 SEIHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNP 540

Query: 541 GVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIP 588
           GVW FHCHIEPHL MGMGV+FAE V  V  IPP ALACG TA  +  P +P
Sbjct: 541 GVWAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTALVKNYPRLP 587

BLAST of CsaV3_1G042190 vs. ExPASy Swiss-Prot
Match: P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)

HSP 1 Score: 855.5 bits (2209), Expect = 3.9e-247
Identity = 403/566 (71.20%), Postives = 462/566 (81.63%), Query Frame = 0

Query: 15  FLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIVMGINGTFPGPPIRA 74
           FL++FF F +   FGI  S   SQ R+YK+EVEYMFW+PDC ENIVMGING FPGP IRA
Sbjct: 12  FLILFF-FGLILAFGI--SSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIRA 71

Query: 75  NVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPINPGENFTYEFKVDKP 134
           N  DTVVVE+IN L TEGVVIHWHGILQRG+PWADGTASISQC INPGE F Y F VD P
Sbjct: 72  NAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNP 131

Query: 135 GTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHASVHEQEVGLKS 194
           GT+FYHGH GMQR+AGLYGSLIVD  +GKKEPF YD EINLLLSDWWH S+H+QEVGL S
Sbjct: 132 GTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSS 191

Query: 195 NPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCEWK--DQCAPFVLKVEPNKTYRIRI 254
            P+RW+GEPQTIL+NG GQF+C+   KY+++ E C+ K  + CAP++  V P KTYRIRI
Sbjct: 192 KPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMPKKTYRIRI 251

Query: 255 ASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLVTTNQSTTQNYWA 314
           ASTT+LAALNFAI NH +LVVEADGNYVQ F TSDIDIYSGESYSVL+TT+Q+ ++NYW 
Sbjct: 252 ASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWV 311

Query: 315 SIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNFTYRVLASAMNTT 374
           S+G RGR P T P L L NYLPNSV+KLP SPPPE P W+D+DRSKNFTYR+ A AM + 
Sbjct: 312 SVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITA-AMGSP 371

Query: 375 KPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGLNSTFNQTAPPET 434
           KPP    RRI LLNTQN++NG ++KWAIN++SL LP TPYLGA+KF L   F+Q  PPE 
Sbjct: 372 KPPVKSNRRIFLLNTQNVING-YVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEV 431

Query: 435 FPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEIHPWHLHGHDFWV 494
           FP++Y+I  PP N  T  GN VY+F+ GEIVDVILQNAN +  N SEIHPWHLHGHDFWV
Sbjct: 432 FPEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWV 491

Query: 495 LGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMG 554
           LGYG+GKF+ E+E+ LNLKNPPL+NTVVIFP+GWTAIRFVADNPGVW FHCHIEPHL MG
Sbjct: 492 LGYGDGKFTAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMG 551

Query: 555 MGVIFAEAVEKVREIPPYALACGRTA 579
           MGV+FAE VEKV  IP  ALACG TA
Sbjct: 552 MGVVFAEGVEKVGRIPTKALACGGTA 572

BLAST of CsaV3_1G042190 vs. ExPASy Swiss-Prot
Match: P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 4.5e-243
Identity = 388/544 (71.32%), Postives = 448/544 (82.35%), Query Frame = 0

Query: 37  SQTRYYKFEVEYMFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIH 96
           SQ R+YK+EVEYMFW+P+C ENIVMGING FPGP IRAN  D+VVVE+ N L TEGVVIH
Sbjct: 1   SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60

Query: 97  WHGILQRGSPWADGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLI 156
           WHGILQRG+PWADGTASISQC INPGE F Y F VD PGT+FYHGH GMQR+AGLYGSLI
Sbjct: 61  WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120

Query: 157 VDLEEGKKEPFQYDEEINLLLSDWWHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNC 216
           VD  +GKKEPF YD EINLLLSDWWH S+H+QEVGL S P+RW+GEPQTIL+NG GQF+C
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDC 180

Query: 217 TSLEKYNTSSEHCEWK--DQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVE 276
           +   KY+++ E C+ K  + CAP++  V P KTYRIRIASTT+LAALNFAI NH +LVVE
Sbjct: 181 SIAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVE 240

Query: 277 ADGNYVQQFVTSDIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLP 336
           ADGNYVQ F TSDIDIYSGESYSVL+TT+Q+ ++NYW S+G R R P T P L L NYLP
Sbjct: 241 ADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLP 300

Query: 337 NSVAKLPISPPPEIPKWNDYDRSKNFTYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGN 396
           NSV+KLP SPPP+ P W+D+DRSKNFTYR+ A AM + KPP  F RRI LLNTQN++NG 
Sbjct: 301 NSVSKLPTSPPPQTPAWDDFDRSKNFTYRITA-AMGSPKPPVKFNRRIFLLNTQNVING- 360

Query: 397 FIKWAINNISLTLPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSV 456
           ++KWAIN++SL LP TPYLGA+K+ L   F+Q  PPE FP++Y+I  PP N  T  GN V
Sbjct: 361 YVKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGV 420

Query: 457 YRFRSGEIVDVILQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPP 516
           Y+F+ GE+VDVILQNAN +  N SE HPWHLHGHDFWVLGYG+GKFS E+E+ LNLKNPP
Sbjct: 421 YQFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSAEEESSLNLKNPP 480

Query: 517 LKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALAC 576
           L+NTVVIFP+GWTAIRFVADNPGVW FHCHIEPHL MGMGV+FAE VEKV  IP  ALAC
Sbjct: 481 LRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALAC 540

Query: 577 GRTA 579
           G TA
Sbjct: 541 GGTA 542

BLAST of CsaV3_1G042190 vs. ExPASy Swiss-Prot
Match: Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 9.8e-214
Identity = 351/566 (62.01%), Postives = 427/566 (75.44%), Query Frame = 0

Query: 15  FLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIVMGINGTFPGPPIRA 74
           FL  F +  I      + S   ++TR++K++VEY+ WSPD  E++VMGING FPGP IRA
Sbjct: 6   FLFFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRA 65

Query: 75  NVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPINPGENFTYEFKVDKP 134
              DTV V + N L TEGVVIHWHGI Q G+PWADGTA+ISQC INPGE F Y FKVDK 
Sbjct: 66  KAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKA 125

Query: 135 GTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHASVHEQEVGLKS 194
           GTYFYHGH+GMQR+AGLYGSLIV++ EG+KEPF YD E NLLLSDWWH   HEQEV L S
Sbjct: 126 GTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSS 185

Query: 195 NPMRWVGEPQTILINGTGQFNCTSLEKYNTSS-EHCEWK--DQCAPFVLKVEPNKTYRIR 254
           NP+RW+GEPQT+L+NG GQ+NC+   +++      C+ +  +Q AP +L+V PNK YR+R
Sbjct: 186 NPLRWIGEPQTLLLNGRGQYNCSLAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIYRLR 245

Query: 255 IASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLVTTNQSTTQNYW 314
           +ASTT+L +L+ AI  H M+VVEADGNYVQ F   D+DIYSGESYSVL  T+Q  T+NYW
Sbjct: 246 VASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKNYW 305

Query: 315 ASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNFTYRVLASAMNT 374
            SI VRGREPKT   L L NYLPNS +K P  PPP  P WNDY+ SK+F+ ++ A  M +
Sbjct: 306 ISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFA-LMGS 365

Query: 375 TKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGLNSTFNQTAPPE 434
            KPPP   RRI LLNTQN ++G + KWAINN+SL LP+  YLG+I++G+N+ F+   PP+
Sbjct: 366 PKPPPQNHRRIILLNTQNKIDG-YTKWAINNVSLVLPTQLYLGSIRYGINA-FDTKPPPD 425

Query: 435 TFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEIHPWHLHGHDFW 494
            FPK+Y++    PN N+  GN VY  +    +D+ILQNANAL+ + SEIHPWHLHGHDFW
Sbjct: 426 NFPKDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHDFW 485

Query: 495 VLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDM 554
           VLGYGEGKFS++D  K NLKNPPL+NT VIFPFGWTA+RFV DNPGVW FHCHIEPHL M
Sbjct: 486 VLGYGEGKFSEKDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPHLHM 545

Query: 555 GMGVIFAEAVEKVREIPPYALACGRT 578
           GMGVIFAE V  V++IP  ALACG T
Sbjct: 546 GMGVIFAEGVHLVKKIPKEALACGLT 568

BLAST of CsaV3_1G042190 vs. ExPASy Swiss-Prot
Match: M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 5.6e-209
Identity = 337/547 (61.61%), Postives = 415/547 (75.87%), Query Frame = 0

Query: 35  AVSQTRYYKFEVEYMFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVV 94
           A +  R Y +EVEY F  PDC E +VM +NG FPGP I A   DT+VV + N L+TEG+V
Sbjct: 16  ASAAVREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHLTNKLATEGLV 75

Query: 95  IHWHGILQRGSPWADGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGS 154
           IHWHGI Q GSPWADG A ++QC I+PGE FTY F VDKPGT+FYHGH+GMQR+AGLYGS
Sbjct: 76  IHWHGIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHGHYGMQRSAGLYGS 135

Query: 155 LIVDLEEGKKEPFQYDEEINLLLSDWWHASVHEQEVGLKSNPMRWVGEPQTILINGTGQF 214
           LI+D+ +GKKEP +YD E NLLLSDWWH  V  QE+GL S PMRW+GE Q+ILING GQF
Sbjct: 136 LIIDVAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSSRPMRWIGEAQSILINGRGQF 195

Query: 215 NCTSLEKY-NTSSEHCEWK--DQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTML 274
           NC+   ++ +TS   C +K  DQCAP  L VEPNKTYRIR+AS+T+LA+LNFA+  H ++
Sbjct: 196 NCSLAAQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTALASLNFAVQGHKLV 255

Query: 275 VVEADGNYVQQFVTSDIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFN 334
           VVEADGNY+  F T DIDIYSGE+YSVL+TT+Q  +QNY+ + GVRGR+P T PAL + N
Sbjct: 256 VVEADGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGVRGRKPNTPPALTVLN 315

Query: 335 YLPNSVAKLPISPPPEIPKWNDYDRSKNFTYRVLASAMNTTKPPPIFTRRISLLNTQNLM 394
           Y+    ++LP SPPPE P+WND+DRSKNF+ ++ A AM +  PP  F  R+ LLNTQNL+
Sbjct: 316 YVTAPSSQLPTSPPPETPRWNDFDRSKNFSKKIFA-AMGSPSPPETFDERLILLNTQNLI 375

Query: 395 NGNFIKWAINNISLTLPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTG 454
            G F KWAINN+SL +P TPYLG++K+ L + FN+++PP+ +P +Y+I  PP N N   G
Sbjct: 376 EG-FTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPVDYDIMTPPRNRNAKQG 435

Query: 455 NSVYRFRSGEIVDVILQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFS-DEDETKLNL 514
           N    F     VDVILQNAN L+ N SEIHPWHLHGHDFWVLGYGEGKF    DE   NL
Sbjct: 436 NVSCVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLGYGEGKFKPGVDEKTYNL 495

Query: 515 KNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPY 574
           KNPPL+NTV ++P+GWTA+RFV DNPGVW FHCHIEPHL MGMGV+FAE + ++ ++P  
Sbjct: 496 KNPPLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGKVPDE 555

Query: 575 ALACGRT 578
           AL CG T
Sbjct: 556 ALGCGLT 560

BLAST of CsaV3_1G042190 vs. ExPASy TrEMBL
Match: A0A0A0M2J6 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G602530 PE=3 SV=1)

HSP 1 Score: 1376.3 bits (3561), Expect = 0.0e+00
Identity = 662/673 (98.37%), Postives = 662/673 (98.37%), Query Frame = 0

Query: 1   MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIV 60
           MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIV
Sbjct: 1   MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIV 60

Query: 61  MGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPIN 120
           MGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPIN
Sbjct: 61  MGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPIN 120

Query: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180
           PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW
Sbjct: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180

Query: 181 WHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCEWKDQCAPFVL 240
           WHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCEWKDQCAPFVL
Sbjct: 181 WHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCEWKDQCAPFVL 240

Query: 241 KVEPNKTYRIRIASTTSLAALNFAIS-----------NHTMLVVEADGNYVQQFVTSDID 300
           KVEPNKTYRIRIASTTSLAALNFAIS           NHTMLVVEADGNYVQQFVTSDID
Sbjct: 241 KVEPNKTYRIRIASTTSLAALNFAISKKFHHFSFLFQNHTMLVVEADGNYVQQFVTSDID 300

Query: 301 IYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIP 360
           IYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIP
Sbjct: 301 IYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIP 360

Query: 361 KWNDYDRSKNFTYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPS 420
           KWNDYDRSKNFTYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPS
Sbjct: 361 KWNDYDRSKNFTYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPS 420

Query: 421 TPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQN 480
           TPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQN
Sbjct: 421 TPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQN 480

Query: 481 ANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAI 540
           ANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAI
Sbjct: 481 ANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAI 540

Query: 541 RFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPRP 600
           RFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPRP
Sbjct: 541 RFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPRP 600

Query: 601 NPPGLDNPYYIIWIGLVIFLCVIGLCYVCLYRIRFNNLSWRPYLGISESQSNQIEFSDLS 660
           NPPGLDNPYYIIWIGLVIFLCVIGLCYVCLYRIRFNNLSWRPYLGISESQSNQIEFSDLS
Sbjct: 601 NPPGLDNPYYIIWIGLVIFLCVIGLCYVCLYRIRFNNLSWRPYLGISESQSNQIEFSDLS 660

Query: 661 QSNPNPNPTHEEP 663
           QSNPNPNPTHEEP
Sbjct: 661 QSNPNPNPTHEEP 673

BLAST of CsaV3_1G042190 vs. ExPASy TrEMBL
Match: A0A1S3BQ56 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492477 PE=3 SV=1)

HSP 1 Score: 1229.2 bits (3179), Expect = 0.0e+00
Identity = 599/674 (88.87%), Postives = 625/674 (92.73%), Query Frame = 0

Query: 1   MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIV 60
           MAKVANQP L P+PFLV FFV SI FGFGITLSKAVSQTRYYKFEV+YMFWSPDC+ENIV
Sbjct: 1   MAKVANQP-LFPLPFLV-FFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIV 60

Query: 61  MGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPIN 120
           +GINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRG+PWADGTASISQCPIN
Sbjct: 61  LGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPIN 120

Query: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180
           PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW
Sbjct: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180

Query: 181 WHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCEWKDQCAPFVL 240
           WH S+ EQEVGLKSNPMRW+GE Q ILINGTGQFNCTSL+KYNTSSEHCEWKDQCAPFVL
Sbjct: 181 WHTSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVL 240

Query: 241 KVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLV 300
           KVEPNKTYRIRIASTTSL+ALNFAI+NHTMLVVEADGNYV  F TSDIDIYSGESYSVLV
Sbjct: 241 KVEPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLV 300

Query: 301 TTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNF 360
           TTNQ+TTQNYWASIGVRGR+PKTSPALLLFNYLPNSVAKLPISPPPE PKW+DYDRSK+F
Sbjct: 301 TTNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSF 360

Query: 361 TYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGL 420
           T RVLASAMNTTKPPPIFTRRISLLNTQN +NG F KWAINNISLTLPSTPYLGAIKFGL
Sbjct: 361 TNRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGL 420

Query: 421 NSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEI 480
           NSTFNQT PPETFPKNYNIKIPPPNPNTNTGN VYRFRSGEIVDVILQNANAL+NNTSEI
Sbjct: 421 NSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEI 480

Query: 481 HPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540
           HPWHLHGHDFWVLG+GEGKFS  DETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI
Sbjct: 481 HPWHLHGHDFWVLGFGEGKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540

Query: 541 FHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPRPNP---PGLDNP 600
           FHCHIEPHLDMGMGVIFAEAVE+VREIPPYALACGRTAT  VVPIIP+P P   PG   P
Sbjct: 541 FHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIP 600

Query: 601 YYIIW-IGLVIFLCVIGLCYVCLYRI------RFNNLSWRPYLGISESQSNQIEFSDLSQ 660
           Y+++W IGLVI +C IGL  VCLYRI       FNNLSWRPYLG+S+SQSNQIEFSDLS+
Sbjct: 601 YFMLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQSNQIEFSDLSR 660

Query: 661 SN---PNPNPTHEE 662
           SN      NPT EE
Sbjct: 661 SNLTQEESNPTQEE 672

BLAST of CsaV3_1G042190 vs. ExPASy TrEMBL
Match: Q9ZR59 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=ao3 PE=3 SV=1)

HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 597/674 (88.58%), Postives = 624/674 (92.58%), Query Frame = 0

Query: 1   MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIV 60
           MAKVANQP L P+PFLV FFV SI FGFGITLSKAVSQTRYYKFEV+YMFWSPDC+ENIV
Sbjct: 1   MAKVANQP-LFPLPFLV-FFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIV 60

Query: 61  MGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPIN 120
           +GINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRG+PWADGTASISQCPIN
Sbjct: 61  LGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPIN 120

Query: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180
           PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW
Sbjct: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180

Query: 181 WHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCEWKDQCAPFVL 240
           WH S+ EQEVGLKSNPMRW+GE Q ILINGTGQFNCTSL+KYNTSSEHCEWKDQCAPFVL
Sbjct: 181 WHTSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVL 240

Query: 241 KVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLV 300
           KVEPNKTYRIRIASTTSL+ALNFAI+NHTMLVVEADGNYV  F TSDIDIYSGESYSVLV
Sbjct: 241 KVEPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLV 300

Query: 301 TTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNF 360
           TTNQ+TTQNYWASIGVRGR+PKTSPALLLFNYLPNSVAKLPISPPPE PKW+DYDRSK+F
Sbjct: 301 TTNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSF 360

Query: 361 TYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGL 420
           T RVLASAMNTTKPPPIFTRRISLLNTQN +NG F KWAINNISLTLPSTPYLGAIKFGL
Sbjct: 361 TNRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGL 420

Query: 421 NSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEI 480
           NSTFNQT PPETFPKNYNIKIPPPNPNTNTGN VYRFRSGEIVDVILQNANAL+NNTSEI
Sbjct: 421 NSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEI 480

Query: 481 HPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540
           HPWHLHGHDFWVLG+G+ KFS  DETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI
Sbjct: 481 HPWHLHGHDFWVLGFGDFKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540

Query: 541 FHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPRPNP---PGLDNP 600
           FHCHIEPHLDMGMGVIFAEAVE+VREIPPYALACGRTAT  VVPIIP+P P   PG   P
Sbjct: 541 FHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIP 600

Query: 601 YYIIW-IGLVIFLCVIGLCYVCLYRI------RFNNLSWRPYLGISESQSNQIEFSDLSQ 660
           Y+++W IGLVI +C IGL  VCLYRI       FNNLSWRPYLG+S+SQSNQIEFSDLS+
Sbjct: 601 YFMLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQSNQIEFSDLSR 660

Query: 661 SN---PNPNPTHEE 662
           SN      NPT EE
Sbjct: 661 SNLTQEESNPTQEE 672

BLAST of CsaV3_1G042190 vs. ExPASy TrEMBL
Match: Q9M5B5 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=2C07 PE=2 SV=1)

HSP 1 Score: 940.3 bits (2429), Expect = 4.5e-270
Identity = 448/579 (77.37%), Postives = 499/579 (86.18%), Query Frame = 0

Query: 3   KVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIVMG 62
           KVANQP L PMPF  IFF+FSIFFGFGITLS+A SQTR++K+EVEYMF SPDC EN+VMG
Sbjct: 4   KVANQP-LFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMG 63

Query: 63  INGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPINPG 122
           IN  FPGP IRANV DTVVVE+IN LSTE VVIHWHGILQ+G+PWADGTASISQC INPG
Sbjct: 64  INHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPG 123

Query: 123 ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH 182
           E+FTY+F VDKPGTYFYHGH GMQRAAGLYGSLIVDLEEGKKEPF YDEEINLLLSDWWH
Sbjct: 124 ESFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWH 183

Query: 183 ASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCE--WKDQCAPFVL 242
            SV +QEVGL S+PMRW+GEPQ+ILING GQ NC+   KY  + E C+    ++CAPF+L
Sbjct: 184 ESVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEECAPFIL 243

Query: 243 KVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLV 302
           KV+PNKTYRIRI STTSL+ALNFAI NH +LVVEADGNYVQ F+TSDIDIYSGESYSVL+
Sbjct: 244 KVKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLI 303

Query: 303 TTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNF 362
            T+Q+ +QNYW S+GVRGREPKT P L L NYLPNS +KLPISPPPE PKWNDYDRSKNF
Sbjct: 304 NTDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNF 363

Query: 363 TYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGL 422
           TYR+LA AM + KPP   TRRI LLNTQN MNG + KWAINN+SLTLPSTPYLGAIK G+
Sbjct: 364 TYRILA-AMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGI 423

Query: 423 NSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEI 482
           N TF+Q  PPET+P+NY+I  PPPN NT TGN VYRF+ GEIVDV+LQNAN L++  SEI
Sbjct: 424 NDTFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEI 483

Query: 483 HPWHLHGHDFWVLGYGEGKFSDE-DETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVW 542
           HPWHLHGHDFWVLGYGEGKFS   DETKLNLK+PPL+NTVVIFPFGWT +RFVADNPGVW
Sbjct: 484 HPWHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVW 543

Query: 543 IFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTA 579
            FHCHIEPHL MGMGV+FAE V+KV  IP +A+ACG TA
Sbjct: 544 AFHCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTA 580

BLAST of CsaV3_1G042190 vs. ExPASy TrEMBL
Match: A0A5D3CF11 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00020 PE=3 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 1.1e-268
Identity = 446/579 (77.03%), Postives = 497/579 (85.84%), Query Frame = 0

Query: 3   KVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIVMG 62
           K ANQP L PMPF  IFF+FSIFFGFGITLS+A SQTR++K+EVEYMF SPDC EN+VMG
Sbjct: 4   KDANQP-LFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMG 63

Query: 63  INGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPINPG 122
           IN  FPGP IRANV DT+VVE+IN LSTE VVIHWHGILQ+G+PWADGTASISQC INPG
Sbjct: 64  INHQFPGPTIRANVGDTIVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPG 123

Query: 123 ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH 182
           E+FTY+F VDKPGTYFYHGH GMQRAAGLYGSLIVDLEEGKKEPF YDEEINLLLSDWWH
Sbjct: 124 ESFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWH 183

Query: 183 ASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCE--WKDQCAPFVL 242
            SV +QEVGL S+PMRW+GEPQ+ILING GQ NC+   KY  + E C+    + CAPF+L
Sbjct: 184 ESVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEGCAPFIL 243

Query: 243 KVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLV 302
           KV+PNKTYRIRI STTSL+ALNFAI NH +LVVEADGNYVQ F+TSDIDIYSGESYSVL+
Sbjct: 244 KVKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLI 303

Query: 303 TTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNF 362
            T+Q+ +QNYW S+GVRGREPKT P L L NYLPNS +KLPISPPPE PKWNDYDRSKNF
Sbjct: 304 NTDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNF 363

Query: 363 TYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGL 422
           TYR+LA AM + KPP   TRRI LLNTQN MNG + KWAINN+SLTLPSTPYLGAIK G+
Sbjct: 364 TYRILA-AMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGI 423

Query: 423 NSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEI 482
           N TF+Q  PPET+P+NY+I  PPPN NT TGN VYRF+ GEIVDV+LQNAN L++  SEI
Sbjct: 424 NDTFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEI 483

Query: 483 HPWHLHGHDFWVLGYGEGKFSDE-DETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVW 542
           HPWHLHGHDFWVLGYGEGKFS   DETKLNLK+PPL+NTVVIFPFGWT +RFVADNPGVW
Sbjct: 484 HPWHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVW 543

Query: 543 IFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTA 579
            FHCHIEPHL MGMGV+FAE V+KV  IP +A+ACG TA
Sbjct: 544 AFHCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTA 580

BLAST of CsaV3_1G042190 vs. TAIR 10
Match: AT5G21105.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 730.3 bits (1884), Expect = 1.4e-210
Identity = 333/548 (60.77%), Postives = 418/548 (76.28%), Query Frame = 0

Query: 35  AVSQTRYYKFEVEYMFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVV 94
           A +  R Y +EVEY +WSPDC E  VM +NG FPGP I+A   DT+VV + N L+TEG+V
Sbjct: 33  ASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTEGLV 92

Query: 95  IHWHGILQRGSPWADGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGS 154
           IHWHGI Q GSPWADG A ++QC INPGE FTY F V+KPGT+FYHGH+GMQR+AGLYGS
Sbjct: 93  IHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGLYGS 152

Query: 155 LIVDLEEGKKEPFQYDEEINLLLSDWWHASVHEQEVGLKSNPMRWVGEPQTILINGTGQF 214
           LIVD+ +GK E  +YD E NLLLSDWWH ++  QE+GL S PMRW+GE Q+ILING GQF
Sbjct: 153 LIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRWIGEAQSILINGRGQF 212

Query: 215 NCTSLEKY--NTSSEHCEWK--DQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTM 274
           NC+   ++  NTS   C +K  DQCAP +L VEPNKTYRIR++STT+LA+LN A+  H +
Sbjct: 213 NCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAVQGHKL 272

Query: 275 LVVEADGNYVQQFVTSDIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLF 334
           +VVEADGNY+  F T DIDIYSGESYSVL+TT+Q  +QNY+ S+GVRGR+P T+ AL + 
Sbjct: 273 VVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQALTIL 332

Query: 335 NYLPNSVAKLPISPPPEIPKWNDYDRSKNFTYRVLASAMNTTKPPPIFTRRISLLNTQNL 394
           NY+    +KLP SPPP  P+W+D++RSKNF+ ++  SAM +  PP  + +R+ LLNTQNL
Sbjct: 333 NYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIF-SAMGSPSPPKKYRKRLILLNTQNL 392

Query: 395 MNGNFIKWAINNISLTLPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNT 454
           ++G + KWAINN+SL  P+TPYLG++K+ L   FN+ +PP ++  +Y+I  PPP PNT T
Sbjct: 393 IDG-YTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMNPPPFPNTTT 452

Query: 455 GNSVYRFRSGEIVDVILQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDE-DETKLN 514
           GN +Y F     VDVI+QNAN L    SEIHPWHLHGHDFWVLGYG+GKF    DE   N
Sbjct: 453 GNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYN 512

Query: 515 LKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPP 574
           LKNPPL+NT +++P+GWTAIRFV DNPGVW FHCHIEPHL MGMGV+FAE + ++ ++P 
Sbjct: 513 LKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGKVPD 572

Query: 575 YALACGRT 578
            AL CG T
Sbjct: 573 EALGCGLT 578

BLAST of CsaV3_1G042190 vs. TAIR 10
Match: AT5G21100.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 689.5 bits (1778), Expect = 2.7e-198
Identity = 327/542 (60.33%), Postives = 399/542 (73.62%), Query Frame = 0

Query: 44  FEVEYMFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQR 103
           +EVEY +W PDC E IVM ING FPGP I A   DTV++ V+N LSTEGVVIHWHGI Q+
Sbjct: 28  WEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGDTVIIHVVNKLSTEGVVIHWHGIRQK 87

Query: 104 GSPWADGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGK 163
           G+PWADG A ++QCPINPGE FTY+F VDK GT+FYHGH+GMQR++GLYG LIV      
Sbjct: 88  GTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHFYHGHYGMQRSSGLYGMLIV---RSP 147

Query: 164 KEPFQYDEEINLLLSDWWHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYN 223
           KE   YD E NLLLSDWWH S+H QE+ L S PMRW+GEPQ++LING GQFNC+    +N
Sbjct: 148 KERLIYDGEFNLLLSDWWHQSIHAQELALSSRPMRWIGEPQSLLINGRGQFNCSQAAYFN 207

Query: 224 TSSEH--CEWK--DQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNY 283
              E   C +K  DQCAP  L+VEPN+ YR+RIASTT+LA+LN A+  H ++VVEADGNY
Sbjct: 208 KGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRIASTTALASLNLAVQGHQLVVVEADGNY 267

Query: 284 VQQFVTSDIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAK 343
           V  F  +DID+YSGE+YSVL+ TN   ++ YW S+GVRGREPKT  AL + NY+ ++   
Sbjct: 268 VAPFTVNDIDVYSGETYSVLLKTNALPSKKYWISVGVRGREPKTPQALTVINYV-DATES 327

Query: 344 LPISPPPEIPKWNDYDRSKNFTYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWA 403
            P  PPP  P WND DRSK+F+ ++ A A    KPP     ++ LLNTQNL   ++ KW+
Sbjct: 328 RPSHPPPVTPIWNDTDRSKSFSKKIFA-AKGYPKPPEKSHDQLILLNTQNLYE-DYTKWS 387

Query: 404 INNISLTLPSTPYLGAIKFGLNSTFNQTAP-PETFPKNYNIKIPPPNPNTNTGNSVYRFR 463
           INN+SL++P TPYLG+I++GL S ++  +P  +    NY+I  PPPNPNT  G+ +Y F 
Sbjct: 388 INNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKKLIMDNYDIMKPPPNPNTTKGSGIYNFA 447

Query: 464 SGEIVDVILQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDE-DETKLNLKNPPLKN 523
            G +VDVILQNAN L    SEIHPWH+HGHDFWVLGYGEGKF    DE   NLKNPPL+N
Sbjct: 448 FGIVVDVILQNANVLKGVISEIHPWHIHGHDFWVLGYGEGKFKPGIDEKTFNLKNPPLRN 507

Query: 524 TVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKV--REIPPYALACG 578
           TVV++PFGWTAIRFV DNPGVW FHCHIEPHL MGMGV+F E V+++   EIP  AL CG
Sbjct: 508 TVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFVEGVDRIGKMEIPDEALGCG 563

BLAST of CsaV3_1G042190 vs. TAIR 10
Match: AT5G21105.3 (Plant L-ascorbate oxidase )

HSP 1 Score: 669.5 bits (1726), Expect = 2.8e-192
Identity = 308/508 (60.63%), Postives = 387/508 (76.18%), Query Frame = 0

Query: 35  AVSQTRYYKFEVEYMFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVV 94
           A +  R Y +EVEY +WSPDC E  VM +NG FPGP I+A   DT+VV + N L+TEG+V
Sbjct: 33  ASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTEGLV 92

Query: 95  IHWHGILQRGSPWADGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGS 154
           IHWHGI Q GSPWADG A ++QC INPGE FTY F V+KPGT+FYHGH+GMQR+AGLYGS
Sbjct: 93  IHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGLYGS 152

Query: 155 LIVDLEEGKKEPFQYDEEINLLLSDWWHASVHEQEVGLKSNPMRWVGEPQTILINGTGQF 214
           LIVD+ +GK E  +YD E NLLLSDWWH ++  QE+GL S PMRW+GE Q+ILING GQF
Sbjct: 153 LIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRWIGEAQSILINGRGQF 212

Query: 215 NCTSLEKY--NTSSEHCEWK--DQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTM 274
           NC+   ++  NTS   C +K  DQCAP +L VEPNKTYRIR++STT+LA+LN A+  H +
Sbjct: 213 NCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAVQGHKL 272

Query: 275 LVVEADGNYVQQFVTSDIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLF 334
           +VVEADGNY+  F T DIDIYSGESYSVL+TT+Q  +QNY+ S+GVRGR+P T+ AL + 
Sbjct: 273 VVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQALTIL 332

Query: 335 NYLPNSVAKLPISPPPEIPKWNDYDRSKNFTYRVLASAMNTTKPPPIFTRRISLLNTQNL 394
           NY+    +KLP SPPP  P+W+D++RSKNF+ ++  SAM +  PP  + +R+ LLNTQNL
Sbjct: 333 NYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIF-SAMGSPSPPKKYRKRLILLNTQNL 392

Query: 395 MNGNFIKWAINNISLTLPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNT 454
           ++G + KWAINN+SL  P+TPYLG++K+ L   FN+ +PP ++  +Y+I  PPP PNT T
Sbjct: 393 IDG-YTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMNPPPFPNTTT 452

Query: 455 GNSVYRFRSGEIVDVILQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDE-DETKLN 514
           GN +Y F     VDVI+QNAN L    SEIHPWHLHGHDFWVLGYG+GKF    DE   N
Sbjct: 453 GNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYN 512

Query: 515 LKNPPLKNTVVIFPFGWTAIRFVADNPG 538
           LKNPPL+NT +++P+GWTAIRFV DNPG
Sbjct: 513 LKNPPLRNTAILYPYGWTAIRFVTDNPG 538

BLAST of CsaV3_1G042190 vs. TAIR 10
Match: AT4G39830.1 (Cupredoxin superfamily protein )

HSP 1 Score: 536.6 bits (1381), Expect = 2.9e-152
Identity = 266/570 (46.67%), Postives = 359/570 (62.98%), Query Frame = 0

Query: 16  LVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIVMGINGTFPGPPIRAN 75
           L++    ++FF   +   K     R +K+EV+Y F SPDC E +V+ ING FPGP I+A 
Sbjct: 16  LMVLCFIALFFSSVLCQGK----IRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQ 75

Query: 76  VNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPINPGENFTYEFKVDKPG 135
             DT+VVE+ N+  TE V +HWHGI Q G+PW DG   ++QCPI PGE F Y+F VD+PG
Sbjct: 76  QGDTIVVELKNSFMTENVAVHWHGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPG 135

Query: 136 TYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHASVHEQEVGLKSN 195
           TY YH H+GMQR +GL G + V     + EPF YD + N LL+DW+H S+ E+  GL S 
Sbjct: 136 TYMYHSHYGMQRESGLIGMIQVSPPATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLASI 195

Query: 196 PMRWVGEPQTILINGTGQFNCTS--LEKYNTSSEHCEWKD-QCAPFVLKVEPNKTYRIRI 255
           P +WVGEPQ+++I G G+FNC++      +  S  C   +  C+ F+L V P KTYR+RI
Sbjct: 196 PFKWVGEPQSLMIQGRGRFNCSNNLTTPPSLVSGVCNVSNADCSRFILTVIPGKTYRLRI 255

Query: 256 ASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLVTTNQSTTQNYWA 315
            S T+L+AL+F I  H + VVEADG+YV+ F   ++ +YSGE+YSVL+  +Q+  +NYW 
Sbjct: 256 GSLTALSALSFQIEGHNLTVVEADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWI 315

Query: 316 SIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPE--IPKWNDYDRSKNFTYRVLASAMN 375
           +  +  R   T PA  + NY PN   + P +      +P+WND       +  + A    
Sbjct: 316 TSSIVSRPATTPPATAVLNYYPNHPRRRPPTSESSNIVPEWNDTRSRLAQSLAIKARRGF 375

Query: 376 TTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGLNSTFNQ--TA 435
               P    + I LLNTQN +NG + +W++NN+S   P TPYL A+K  L + F+   TA
Sbjct: 376 IHALPENSDKVIVLLNTQNEVNG-YRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTA 435

Query: 436 PPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEIHPWHLHGH 495
           P     +NY+I   P N N  T + +YR R    VDVILQNAN ++ N SE HPWHLHGH
Sbjct: 436 PENYDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGH 495

Query: 496 DFWVLGYGEGKFSD-EDETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEP 555
           DFWVLGYGEGKF++ ED  + N  +P  KNTV + PFGWTA+RF ADNPGVW FHCHIE 
Sbjct: 496 DFWVLGYGEGKFNESEDPKRYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIES 555

Query: 556 HLDMGMGVIFAEAVEKVREIPPYALACGRT 578
           H  MGMG++F   ++KV  +P   + CG+T
Sbjct: 556 HFFMGMGIVFESGIDKVSSLPSSIMGCGQT 580

BLAST of CsaV3_1G042190 vs. TAIR 10
Match: AT5G21105.2 (Plant L-ascorbate oxidase )

HSP 1 Score: 479.6 bits (1233), Expect = 4.2e-135
Identity = 223/379 (58.84%), Postives = 287/379 (75.73%), Query Frame = 0

Query: 204 QTILINGTGQFNCTSLEKY--NTSSEHCEWK--DQCAPFVLKVEPNKTYRIRIASTTSLA 263
           ++ILING GQFNC+   ++  NTS   C +K  DQCAP +L VEPNKTYRIR++STT+LA
Sbjct: 11  KSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALA 70

Query: 264 ALNFAISNHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLVTTNQSTTQNYWASIGVRGR 323
           +LN A+  H ++VVEADGNY+  F T DIDIYSGESYSVL+TT+Q  +QNY+ S+GVRGR
Sbjct: 71  SLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGR 130

Query: 324 EPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNFTYRVLASAMNTTKPPPIFT 383
           +P T+ AL + NY+    +KLP SPPP  P+W+D++RSKNF+ ++  SAM +  PP  + 
Sbjct: 131 KPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIF-SAMGSPSPPKKYR 190

Query: 384 RRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNI 443
           +R+ LLNTQNL++G + KWAINN+SL  P+TPYLG++K+ L   FN+ +PP ++  +Y+I
Sbjct: 191 KRLILLNTQNLIDG-YTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDI 250

Query: 444 KIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEIHPWHLHGHDFWVLGYGEGK 503
             PPP PNT TGN +Y F     VDVI+QNAN L    SEIHPWHLHGHDFWVLGYG+GK
Sbjct: 251 MNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGK 310

Query: 504 FSDE-DETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFA 563
           F    DE   NLKNPPL+NT +++P+GWTAIRFV DNPGVW FHCHIEPHL MGMGV+FA
Sbjct: 311 FKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFA 370

Query: 564 EAVEKVREIPPYALACGRT 578
           E + ++ ++P  AL CG T
Sbjct: 371 EGLNRIGKVPDEALGCGLT 387