CsaV3_1G037510 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_1G037510
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Descriptionberberine bridge enzyme-like 18
Locationchr1: 23433331 .. 23437097 (-)
RNA-Seq ExpressionCsaV3_1G037510
SyntenyCsaV3_1G037510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGATAGTTATGGAGAGAAGGGATGTTTTACTGAAAAGATACATTTCCATTGATTCATTGTAATGATGTGGTAAAATCAGAAAAGACGTAGTCAAACATCATCCTAACAATAATATAACAATGACGAAGGGTGAGAGATAGAGGTTAAATAGGCATTTTCTTAAAATGCAATGGCAATATGTAAAGTGAAAAATTAGAGTATACCTTTTTAAATTATGATAGAGATAGAGATATTACTTCATTAGCGAGAGAGATGGAGATAGATATTATTTCCCCCAATAATTTGCAATTTAACATTATTTAAGTAATAATGAGTTTGAAATCTCCACACAAATTAAAACCACACCATAACCATAAGTAGTGGAAATTTGAATCACAAAAACCTCAAATAAAATCGCCATGAAGAAGTCTTCTCCATCAATCCATCTTTCTCTAATTGCTTTCATTGTTATATGTTCATCATTTTCATTATTCAATGCAGCTTCTTCTGACAAACATGAAGAGTTTCTTCAATGTCTACTTCATCACTCTCCACATAGTTCATCTATTGCCAAACTTATTTACACTCAAAACAACTCTTCCTATTCCTCAGTCCTAAACTTGTCCATTAGAAACCACAGATTCTCAACTCCCAACACCCCAAAACCAATCCTCATCATAACCCCATCAAATATTTCCCACATTCAAGCAGCTGTCATTTGCTCCAAGTCTCAAGGCCTTCAAATCCGAATCCGAAGCGGTGGTCACGACTTCGAGGGTCTTTCTTACGTTGCCTACCTCCCATTCATCGTTGTTGATCTTATTAATCTAAGGTCTATCACGGTCGACGTCAAAAGAAGAACCGCATGGGTTCAGTCAGCGGCTACTCTAGGAGAACTTTACTACAGAATTGCTGAAAAAAGTCCAACCTTGACCTTTCCGGGAGGGGCTTGTCCGACTGTTTGTTTTGGTGGGTACCTTAGTGGTGGGGGATATGGCCTGTTACTGAGGAAATATGGTTTGGCAGCCGATAATGTAATAGACGCTTATTTGGTTGATGCAAATGGGGAGTTTCATGATAGGGAATCGATGGGAGAGGATTTGTTTTGGGCAATTAGAGGCGGCGGAGGAGGGAGCTTTGGAATTGTGGTGGCGTGGAAGGTCAAGCTGGTTCCGGTTCCGGCGACGGTGACGTTTTGCTCAAGTAGCAGAACTTTCGAAGAAGATGCAATCAATCTAATCCATCAGTGGCAATATGTGGGTTACAAATTGGAAAAAAATATAGCACATGGAGTCCTTACCATTGGTATTTCTCTTTCAACGTATTTTTTTAATATTATTTATACAATTATTTTTTTCTTTGGAATAATTTGTTTCTTTTTTCATCATTTAATTTTTTCACTGAAGATCACACTACACCAGATTAAAGATTGAAAACAATAAAAAAGAAATAGGCTTTTGGAAAAAATACCTTGTCTTAATTTTTTCATGTTTTAAACTTTTTTTTTCACTCACACTTCGTCTTATTTCTCTTTCCATTTTCGTTCTTCTTCATTCACATCCCTCCTCTCTCCTCCATCCTTGGTTCTTCTTCTTTTCTCTCACCCTACTTTCTCTCCTCGTTCTTTAGATTCATTTCCAAGAACAAAAATGGAGGAGAGAGAATAGACTGTGAGACAAGGACGACGAAGATGGGGGAGAGAGAAAAATAAAAATAGTTTTTTTTTTTTGCCATCTCCTGCTACCATCTAAGAGTTAGTCTAATTGTTTTTTAAAATTCTTGTCAGGGGATTATTATCCACCTCTTAGAAACTCAGGCTAATGAAGGGGTTTTCTCAAAAACAGACAAAAATAAAATAAAATAAAATAAACAAAATATTTACTTAAAGAAATTAGAAATTCATTGAACATGATCTTTTTATTGATTTTTCTATGAAGATAATGAAAGAGTGAGTTTTACTCTTTTTTCTTTCTTTATTTCTTTTAACAGACAACATATTAGAGAAAAATGGGTCCAATATATTGCAACTTTAGATTCATTCTTATGTATTTGTGTGATAAATTGAAACTTCAGCCCTACGAGAAAAAAGTTTAAAGTTTAAAAAATAAAGTTTGGACAATATAATTGGTGATTCCAATTTCTTCTTTCTTTTTTTTTTTTTTTTTTTTTTGTCATGGATTATAAGGAAGGTAGTCTACAGAATTAAACTTTAATTTTATTCTTTTCAATTAAAGCAATAAACTTATAGAAGTGGCAACATTATTACAATTTAATTTTGTTCACATTTTCTTTTAATTTCCTTATGAATTTGAGCTAGGCATGGTTCCTCCTTCTTTCATTAGGATTGTTATTTATTTTAAGACCTTTAATTTCTTTGCTTTTATTCTAGACATGAAAGATTTTATTTGAACAAATAACATGCCAGGTTTGAAGATCGAATAATCTTCGACCTCTAAAAAAATCAAGTCAATTATTGTTAAAGAAAAGAAAGTGAATTTTGACTAGTTGTTTGAGATTTTGAATGTGGATTTATACGTGTAGAACAATAGGATTTTGAGAGAGTTTACTGGGTAATTGTTGATATATATGATATATATATATATAGGTGGACAGATGACAAGTGAAGTAGATGGGAAAGTAAAGCCAGTAGCTATATTCTACACTTCATTTCTTGGGAAGGCAAATAAGGCAGTGAAAATCCTAAAAGAAAAATTTCCTCAATTGGGTTTAAAAAAGGAAGAGTGCAAAGAAGCAAGTTGGGTTGAATCAGTTGTTATTGCTGCCAATGATTTTACGGTTGGAGAGCCCGTTGAAGCCTTGCTCAATAGATCAGCATTGATTCCACCTATCACTAGCAAAAAAGTAAAAATAAAATCTGATTATGTAAAGGAACCCATGCCCAAAGTTGCAATTGAAGGCATATGGAATAGAGTAAATAACTCTCAAGACATAGGAGGAATAAACGTTCTATTTGTTCCTTATGGAGGGAGAATGAGTGAGATTTCTGAGTCCGAAATTTCTTTCTCACATAGAGCTGGAAACTTGTTCAAAATTGCATACCTTACTGGATGGGAAGACCCAAGTATGGATGTAGAGACAAGACATCTAAATTGGATACGAGAAATTTACAGTTACATGGCTCCTTTTGTTTCCAAATCACCTAGAAGTGCATACGTCAATTACAGAGATCTTGATATTGGATCCAACTCTGACAAGTACGGAAACATCGTCACAAACCATGACCAAGCTAGCAGTTGGGGTTTGAAATACTATGGTAACAATTTTAATAGGTTGGTACAAATTAAAACCAAGGTGGATCCCTATAATTTCTTTAGGCATGAGCAAAGCATTCCAATCGCTCTTTCTCAAGAAGATAATTTAGTAAAATTAGTTTAGTAGTTTGATATTTCGTTTATGTGATTGTAACTGAATTATGCCCCAACCTATTATTATAGTGTGTGCAGAATAAGGGGTCGCTTGTGTCCTTTATGCATCAAAATATCTTTTTTTTTCTTTTTTCATGGGTTAAGTTAACCCTATAATCAATCAGATTTGAGAAATAATTCTAAATGTTCCTAGACTAGTTGACCAACAAAAAAAAAAAAAGATGATGTCGCACCTAATTAAATAATGAGATTTTGTTGATGTTGAATTTTATAATAAGTTGGCAAAAAAAATTATATTATTTATGTGTTTCATGTATCATTTTCTTTCTCTATTCTGCGACGTCTTGATCTTCCTCCTGCTCATCCTGTTGCCACCGTAATGGTATAGTTTGCATTTTCC

mRNA sequence

ATGACGAAGGCTTCTTCTGACAAACATGAAGAGTTTCTTCAATGTCTACTTCATCACTCTCCACATAGTTCATCTATTGCCAAACTTATTTACACTCAAAACAACTCTTCCTATTCCTCAGTCCTAAACTTGTCCATTAGAAACCACAGATTCTCAACTCCCAACACCCCAAAACCAATCCTCATCATAACCCCATCAAATATTTCCCACATTCAAGCAGCTGTCATTTGCTCCAAGTCTCAAGGCCTTCAAATCCGAATCCGAAGCGGTGGTCACGACTTCGAGGGTCTTTCTTACGTTGCCTACCTCCCATTCATCGTTGTTGATCTTATTAATCTAAGGTCTATCACGGTCGACGTCAAAAGAAGAACCGCATGGGTTCAGTCAGCGGCTACTCTAGGAGAACTTTACTACAGAATTGCTGAAAAAAGTCCAACCTTGACCTTTCCGGGAGGGGCTTGTCCGACTGTTTGTTTTGGTGGGTACCTTAGTGGTGGGGGATATGGCCTGTTACTGAGGAAATATGGTTTGGCAGCCGATAATGTAATAGACGCTTATTTGGTTGATGCAAATGGGGAGTTTCATGATAGGGAATCGATGGGAGAGGATTTGTTTTGGGCAATTAGAGGCGGCGGAGGAGGGAGCTTTGGAATTGTGGTGGCGTGGAAGGTCAAGCTGGTTCCGGTTCCGGCGACGGTGACGTTTTGCTCAAGTAGCAGAACTTTCGAAGAAGATGCAATCAATCTAATCCATCAGTGGCAATATGTGGGTTACAAATTGGAAAAAAATATAGCACATGGAGTCCTTACCATTGGTGGACAGATGACAAGTGAAGTAGATGGGAAAGTAAAGCCAGTAGCTATATTCTACACTTCATTTCTTGGGAAGGCAAATAAGGCAGTGAAAATCCTAAAAGAAAAATTTCCTCAATTGGGTTTAAAAAAGGAAGAGTGCAAAGAAGCAAGTTGGGTTGAATCAGTTGTTATTGCTGCCAATGATTTTACGGTTGGAGAGCCCGTTGAAGCCTTGCTCAATAGATCAGCATTGATTCCACCTATCACTAGCAAAAAAGTAAAAATAAAATCTGATTATGTAAAGGAACCCATGCCCAAAGTTGCAATTGAAGGCATATGGAATAGAGTAAATAACTCTCAAGACATAGGAGGAATAAACGTTCTATTTGTTCCTTATGGAGGGAGAATGAGTGAGATTTCTGAGTCCGAAATTTCTTTCTCACATAGAGCTGGAAACTTGTTCAAAATTGCATACCTTACTGGATGGGAAGACCCAAGTATGGATGTAGAGACAAGACATCTAAATTGGATACGAGAAATTTACAGTTACATGGCTCCTTTTGTTTCCAAATCACCTAGAAGTGCATACGTCAATTACAGAGATCTTGATATTGGATCCAACTCTGACAAGTACGGAAACATCGTCACAAACCATGACCAAGCTAGCAGTTGGGGTTTGAAATACTATGGTAACAATTTTAATAGGTTGGTACAAATTAAAACCAAGGTGGATCCCTATAATTTCTTTAGGCATGAGCAAAGCATTCCAATCGCTCTTTCTCAAGAAGATAATTTAGTAAAATTAGTTTAG

Coding sequence (CDS)

ATGACGAAGGCTTCTTCTGACAAACATGAAGAGTTTCTTCAATGTCTACTTCATCACTCTCCACATAGTTCATCTATTGCCAAACTTATTTACACTCAAAACAACTCTTCCTATTCCTCAGTCCTAAACTTGTCCATTAGAAACCACAGATTCTCAACTCCCAACACCCCAAAACCAATCCTCATCATAACCCCATCAAATATTTCCCACATTCAAGCAGCTGTCATTTGCTCCAAGTCTCAAGGCCTTCAAATCCGAATCCGAAGCGGTGGTCACGACTTCGAGGGTCTTTCTTACGTTGCCTACCTCCCATTCATCGTTGTTGATCTTATTAATCTAAGGTCTATCACGGTCGACGTCAAAAGAAGAACCGCATGGGTTCAGTCAGCGGCTACTCTAGGAGAACTTTACTACAGAATTGCTGAAAAAAGTCCAACCTTGACCTTTCCGGGAGGGGCTTGTCCGACTGTTTGTTTTGGTGGGTACCTTAGTGGTGGGGGATATGGCCTGTTACTGAGGAAATATGGTTTGGCAGCCGATAATGTAATAGACGCTTATTTGGTTGATGCAAATGGGGAGTTTCATGATAGGGAATCGATGGGAGAGGATTTGTTTTGGGCAATTAGAGGCGGCGGAGGAGGGAGCTTTGGAATTGTGGTGGCGTGGAAGGTCAAGCTGGTTCCGGTTCCGGCGACGGTGACGTTTTGCTCAAGTAGCAGAACTTTCGAAGAAGATGCAATCAATCTAATCCATCAGTGGCAATATGTGGGTTACAAATTGGAAAAAAATATAGCACATGGAGTCCTTACCATTGGTGGACAGATGACAAGTGAAGTAGATGGGAAAGTAAAGCCAGTAGCTATATTCTACACTTCATTTCTTGGGAAGGCAAATAAGGCAGTGAAAATCCTAAAAGAAAAATTTCCTCAATTGGGTTTAAAAAAGGAAGAGTGCAAAGAAGCAAGTTGGGTTGAATCAGTTGTTATTGCTGCCAATGATTTTACGGTTGGAGAGCCCGTTGAAGCCTTGCTCAATAGATCAGCATTGATTCCACCTATCACTAGCAAAAAAGTAAAAATAAAATCTGATTATGTAAAGGAACCCATGCCCAAAGTTGCAATTGAAGGCATATGGAATAGAGTAAATAACTCTCAAGACATAGGAGGAATAAACGTTCTATTTGTTCCTTATGGAGGGAGAATGAGTGAGATTTCTGAGTCCGAAATTTCTTTCTCACATAGAGCTGGAAACTTGTTCAAAATTGCATACCTTACTGGATGGGAAGACCCAAGTATGGATGTAGAGACAAGACATCTAAATTGGATACGAGAAATTTACAGTTACATGGCTCCTTTTGTTTCCAAATCACCTAGAAGTGCATACGTCAATTACAGAGATCTTGATATTGGATCCAACTCTGACAAGTACGGAAACATCGTCACAAACCATGACCAAGCTAGCAGTTGGGGTTTGAAATACTATGGTAACAATTTTAATAGGTTGGTACAAATTAAAACCAAGGTGGATCCCTATAATTTCTTTAGGCATGAGCAAAGCATTCCAATCGCTCTTTCTCAAGAAGATAATTTAGTAAAATTAGTTTAG

Protein sequence

MTKASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQEDNLVKLV*
Homology
BLAST of CsaV3_1G037510 vs. NCBI nr
Match: KAE8653320.1 (hypothetical protein Csa_023331 [Cucumis sativus])

HSP 1 Score: 1078.5 bits (2788), Expect = 0.0e+00
Identity = 534/534 (100.00%), Postives = 534/534 (100.00%), Query Frame = 0

Query: 1   MTKASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPI 60
           MTKASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPI
Sbjct: 1   MTKASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPI 60

Query: 61  LIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDV 120
           LIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDV
Sbjct: 61  LIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDV 120

Query: 121 KRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAAD 180
           KRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAAD
Sbjct: 121 KRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAAD 180

Query: 181 NVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSR 240
           NVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSR
Sbjct: 181 NVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSR 240

Query: 241 TFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKA 300
           TFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKA
Sbjct: 241 TFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKA 300

Query: 301 VKILKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKI 360
           VKILKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKI
Sbjct: 301 VKILKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKI 360

Query: 361 KSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFK 420
           KSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFK
Sbjct: 361 KSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFK 420

Query: 421 IAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIV 480
           IAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIV
Sbjct: 421 IAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIV 480

Query: 481 TNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQEDNLVKLV 535
           TNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQEDNLVKLV
Sbjct: 481 TNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQEDNLVKLV 534

BLAST of CsaV3_1G037510 vs. NCBI nr
Match: XP_004135739.1 (berberine bridge enzyme-like 28 [Cucumis sativus])

HSP 1 Score: 1072.8 bits (2773), Expect = 8.2e-310
Identity = 531/531 (100.00%), Postives = 531/531 (100.00%), Query Frame = 0

Query: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 63
           ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII
Sbjct: 28  ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 87

Query: 64  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 123
           TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR
Sbjct: 88  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 147

Query: 124 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 183
           TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI
Sbjct: 148 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 207

Query: 184 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 243
           DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE
Sbjct: 208 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 267

Query: 244 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI 303
           EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI
Sbjct: 268 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI 327

Query: 304 LKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKSD 363
           LKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKSD
Sbjct: 328 LKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKSD 387

Query: 364 YVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIAY 423
           YVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIAY
Sbjct: 388 YVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIAY 447

Query: 424 LTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNH 483
           LTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNH
Sbjct: 448 LTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNH 507

Query: 484 DQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQEDNLVKLV 535
           DQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQEDNLVKLV
Sbjct: 508 DQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQEDNLVKLV 558

BLAST of CsaV3_1G037510 vs. NCBI nr
Match: XP_011659204.2 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653317.1 hypothetical protein Csa_023357 [Cucumis sativus])

HSP 1 Score: 747.3 bits (1928), Expect = 9.3e-212
Identity = 367/525 (69.90%), Postives = 433/525 (82.48%), Query Frame = 0

Query: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 63
           +S   HEEFLQCLLHHSPHS SIAKL YT  N+SYSSVLN SIRN RFS PNTPKP+ II
Sbjct: 27  SSHVNHEEFLQCLLHHSPHSKSIAKLAYTPINTSYSSVLNFSIRNLRFSIPNTPKPLFII 86

Query: 64  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 123
           TP+NISH+QAAVICSKS GLQIRIRSGGHDFEGLSYVAY PFI+VDLI+L S+T++VK+ 
Sbjct: 87  TPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHPFIIVDLIDLSSVTIEVKQS 146

Query: 124 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 183
           TAWVQS ATLGELYYRIAEKS TL FP G  PTV  GG+ SGGG+G LLRKYGLAADNVI
Sbjct: 147 TAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTVGVGGHFSGGGFGTLLRKYGLAADNVI 206

Query: 184 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 243
           DAYLVDANG FHDR+SMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVT C+ SRT E
Sbjct: 207 DAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTISRTLE 266

Query: 244 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI 303
           E+AI L+ QWQYV  KL++++  G+  +GG+++++ D K+ P+A+F++ FLGKA++ + I
Sbjct: 267 EEAIKLVDQWQYVANKLDEDLFLGINLLGGKISAQGD-KINPIALFFSLFLGKADELMAI 326

Query: 304 LKEKFPQLGLKKEECKEASWVESVVIAANDFTV-GEPVEALLNRSALIPPITSKKVKIKS 363
           L + FPQLGL KEECKE SW+ESVV   N   +  +P+E LLNR+    P+ +  +K+KS
Sbjct: 327 LNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPLEVLLNRT----PLATGNIKMKS 386

Query: 364 DYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIA 423
           DYVKEP+PK  IE IW R+  SQDI G N++FVPYGGRMS+IS+SEI FSHRAGNL+KI 
Sbjct: 387 DYVKEPIPKATIEEIWQRL-ESQDIEGANLVFVPYGGRMSQISDSEIPFSHRAGNLYKIG 446

Query: 424 YLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTN 483
           YLTGW +P ++ E  HLNWIR+IY YM PFVSKSPR+AYVNYRDLDIGSNS KYG   T+
Sbjct: 447 YLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYVNYRDLDIGSNS-KYGK--TS 506

Query: 484 HDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQE 528
           + +A  WGLKY+GNNFNR+V +K KVDPYNFFRHEQSIP+ L  +
Sbjct: 507 YKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPVLLKHK 542

BLAST of CsaV3_1G037510 vs. NCBI nr
Match: XP_038895343.1 (cannabidiolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 741.9 bits (1914), Expect = 3.9e-210
Identity = 366/521 (70.25%), Postives = 427/521 (81.96%), Query Frame = 0

Query: 2   TKASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPIL 61
           T +S  K+EEFLQCLLHHSP++ SI  L+Y   NSSYSS+LN SIRN RFSTP  PKP+L
Sbjct: 26  TSSSYIKYEEFLQCLLHHSPNTYSITNLVYAPTNSSYSSLLNFSIRNLRFSTPTIPKPLL 85

Query: 62  IITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVK 121
           IITPSNISHIQAAV+CSKS GLQIRIRSGGHDFEGLSYVAY PFIVVDLINL S+ VDV+
Sbjct: 86  IITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYVAYHPFIVVDLINLSSVAVDVE 145

Query: 122 RRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADN 181
           + TAWVQS ATLGELYYRIAEKS TL FP G CPTV  GG+ SGGGYG LLRKYGLAADN
Sbjct: 146 QSTAWVQSGATLGELYYRIAEKSRTLAFPAGTCPTVGVGGHFSGGGYGTLLRKYGLAADN 205

Query: 182 VIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRT 241
           VIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVT C+++RT
Sbjct: 206 VIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTANRT 265

Query: 242 FEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAV 301
           ++E AI LI QWQYV  KL++++   ++  GG+++++  GK+ P A+F+T FLGKA++ +
Sbjct: 266 WKEGAIKLIDQWQYVANKLDEDLFLSIILTGGKISAQ-GGKINPTALFFTLFLGKADELL 325

Query: 302 KILKEKFPQLGLKKEECKEASWVESVVIAANDFTV-GEPVEALLNRSALIPPITSKKVKI 361
            ILK KFP+L L KE+CKE SW+ES V  AN   +  +P+EA LNR+    P+ +   KI
Sbjct: 326 TILKNKFPELNLAKEDCKETSWIESAVYTANGLQIDDQPLEAFLNRT----PLANGSFKI 385

Query: 362 KSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFK 421
           KSDYVKEP+ K AIEGIW R+  SQDI G+N+  +PYGGRMS+ISESEI F HR GNL+K
Sbjct: 386 KSDYVKEPITKAAIEGIWQRL-KSQDIEGVNLGLIPYGGRMSQISESEIPFPHRIGNLYK 445

Query: 422 IAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIV 481
           I Y+ GWE+  +D E RHLNWIREIYSYM PFVSKSPR+AYVNYRDLDIGSN DKYG   
Sbjct: 446 IGYIIGWEEQGVDAEKRHLNWIREIYSYMTPFVSKSPRAAYVNYRDLDIGSN-DKYGK-- 505

Query: 482 TNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
           T++ +A  WG KY+GNNFNRLV +K +VDPY+FFRHEQSIP
Sbjct: 506 TSYKRACIWGKKYFGNNFNRLVHVKNEVDPYDFFRHEQSIP 537

BLAST of CsaV3_1G037510 vs. NCBI nr
Match: XP_038895262.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 739.6 bits (1908), Expect = 1.9e-209
Identity = 362/523 (69.22%), Postives = 430/523 (82.22%), Query Frame = 0

Query: 2   TKASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPIL 61
           T +S  KHEEFLQCLLHHSP++ SI KL+Y   NSSYSS+LN   RN RFSTPN P P+L
Sbjct: 26  TSSSYIKHEEFLQCLLHHSPNTYSITKLVYAPINSSYSSLLNFPSRNSRFSTPNIPNPLL 85

Query: 62  IITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVK 121
           IITP+NISHIQAA+ICSKS GLQIRIRSGGHDFEGLSYVAY+PFIVVDLINLRS+TVD +
Sbjct: 86  IITPTNISHIQAAIICSKSHGLQIRIRSGGHDFEGLSYVAYVPFIVVDLINLRSVTVDAE 145

Query: 122 RRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADN 181
           + +AWVQS ATLGELYYRIAEKS TL FP G CPTV  GG+ SGGGYG+LLRKYGLAADN
Sbjct: 146 KSSAWVQSGATLGELYYRIAEKSQTLAFPAGVCPTVGVGGHFSGGGYGILLRKYGLAADN 205

Query: 182 VIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRT 241
           VIDAYLVDANGEFHDRESMGEDLFWAIRGGGGG+FG+VVAWKVKLVPVP TVT C++ RT
Sbjct: 206 VIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGNFGVVVAWKVKLVPVPETVTICAAKRT 265

Query: 242 FEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAV 301
            EE AI LI QWQYVG KLE+++   +   GG+ +++  GKV P A+F++ FLGKA++ V
Sbjct: 266 LEEGAIELIDQWQYVGNKLEEDLFLSIFLNGGKNSAQGGGKVNPTALFFSLFLGKADELV 325

Query: 302 KILKEKFPQLGLKKEECKEASWVESVVIAANDFTV-GEPVEALLNRSALIPPITSKKVKI 361
            ILK+KFP+LGL KE+CKE SW+ S V AAN F +  +P+EALLNR+    P+ +  +KI
Sbjct: 326 AILKKKFPELGLTKEDCKEKSWIGSAVSAANRFQIEDQPLEALLNRT----PLANGSLKI 385

Query: 362 KSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFK 421
           KSDYVKE + KV IEGIW R+  SQDI G+ +  +PYGGRM +IS+SEI FSHRAG  ++
Sbjct: 386 KSDYVKELITKVGIEGIWERL-KSQDIEGVTLALIPYGGRMGQISDSEIPFSHRAGIRYQ 445

Query: 422 IAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIV 481
           I Y+ GW++  +D E RH+NWIREIYSYM PFVSKSPR+AYVNYRD DIGSN +KYG + 
Sbjct: 446 IGYIVGWKEQGLDAEKRHINWIREIYSYMTPFVSKSPRAAYVNYRDHDIGSN-NKYGKV- 505

Query: 482 TNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIA 524
            ++ +A  WGLKY+GNNFNRLV++KTKVDP NFFRHEQSIP++
Sbjct: 506 -SYKRARVWGLKYFGNNFNRLVRLKTKVDPSNFFRHEQSIPLS 540

BLAST of CsaV3_1G037510 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 3.4e-140
Identity = 250/520 (48.08%), Postives = 350/520 (67.31%), Query Frame = 0

Query: 6   SDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILIITP 65
           ++  E FL+C   + P++++  KL+YTQNN  Y SVLN +I N RF++  TPKP++I+TP
Sbjct: 28  ANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVTP 87

Query: 66  SNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRRTA 125
           S++SHIQ  ++CSK  GLQIR RSGGHD EG+SY++ +PF++VDL N+RSI +DV  +TA
Sbjct: 88  SHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTA 147

Query: 126 WVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVIDA 185
           WV++ ATLGE+YY + EK+  L+   G CPTVC GG+  GGGYG L+R YGLAADN+IDA
Sbjct: 148 WVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNIIDA 207

Query: 186 YLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFEED 245
           +LV+ +G+  DR+SMGEDLFWA+RGGG  SFGI+VAWK++LV VP +  F         +
Sbjct: 208 HLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPKSTMFSVKKIMEIHE 267

Query: 246 AINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTS-FLGKANKAVKIL 305
            + L+++WQ + YK +K++      I   +T         +  +++S FLG  +  V ++
Sbjct: 268 LVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYFSSVFLGGVDSLVDLM 327

Query: 306 KEKFPQLGLKKEECKEASWVESVVI---AANDFTVGEPVEALLNRSALIPPITSKKVKIK 365
            + FP+LG+KK +C++ SW+++++      N  T     E LL+RSA      +   KIK
Sbjct: 328 NKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLDRSA----GQNGAFKIK 387

Query: 366 SDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKI 425
            DYVK+P+P+     I  ++   +DIG       PYGG M EISES I F HRAG L+++
Sbjct: 388 LDYVKKPIPESVFVQILEKL-YEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYEL 447

Query: 426 AYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVT 485
            Y+  WE    +   +HLNWIR IY++M P+VSK+PR AY+NYRDLDIG N  K  N   
Sbjct: 448 WYICSWE--KQEDNEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPN--- 507

Query: 486 NHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
           N+ QA  WG KY+G NF+RLV++KT VDP NFFR+EQSIP
Sbjct: 508 NYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIP 537

BLAST of CsaV3_1G037510 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 7.6e-140
Identity = 256/524 (48.85%), Postives = 351/524 (66.98%), Query Frame = 0

Query: 6   SDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILIITP 65
           ++  E FL+C   H P++ +  KL+YTQ++  Y S+LN +I+N RF +  TPKP++I+TP
Sbjct: 28  ANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTP 87

Query: 66  SNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRRTA 125
           SN SHIQA ++CSK  GLQIR RSGGHD EG+SY++ +PF+VVDL N+ SI +DV  +TA
Sbjct: 88  SNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTA 147

Query: 126 WVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVIDA 185
           WV++ ATLGE+YY I EK+  L+FPGG CPTV  GG+ SGGGYG L+R YGLAADN+IDA
Sbjct: 148 WVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 186 YLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE-E 245
           +LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+  T  S  +  E  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIH 267

Query: 246 DAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTS--FLGKANKAVK 305
             + L ++WQ + YK +K++      I   +T    GK K     Y S  F G  +  V 
Sbjct: 268 GLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDN-HGKNKTTVHGYFSSIFHGGVDSLVD 327

Query: 306 ILKEKFPQLGLKKEECKEASWVESVVI---AANDFTVGEPVEALLNRSALIPPITSKKV- 365
           ++ + FP+LG+KK +CKE SW+++ +      N  T     E LL+RSA       KK  
Sbjct: 328 LMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSA------GKKTA 387

Query: 366 -KIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGN 425
             IK DYVK+P+P+ A+  I  ++   +D+G    +  PYGG M EISES I F HRAG 
Sbjct: 388 FSIKLDYVKKPIPETAMVKILEKL-YEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGI 447

Query: 426 LFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYG 485
           ++++ Y   WE    +   +H+NW+R +Y++  P+VS++PR AY+NYRDLD+G  +    
Sbjct: 448 MYELWYTASWE--KQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASP 507

Query: 486 NIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
           N   N+ QA  WG KY+G NFNRLV++KTKVDP NFFR+EQSIP
Sbjct: 508 N---NYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIP 538

BLAST of CsaV3_1G037510 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 7.1e-138
Identity = 248/522 (47.51%), Postives = 350/522 (67.05%), Query Frame = 0

Query: 6   SDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILIITP 65
           ++  E FL+CL  + P + + AKL+YTQ++  Y S+LN +++N RF++  TPKP++I TP
Sbjct: 28  ANPQENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTP 87

Query: 66  SNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRRTA 125
            N+SHIQ  ++CSK  GLQIR RSGGHD EG+SY++ +PF++VDL N+ S+ +DV  +TA
Sbjct: 88  LNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTA 147

Query: 126 WVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVIDA 185
           WV+S ATLGE+YY I E +  L+FP G CPTV  GG+ SGGGYG L+R YGLAADN+IDA
Sbjct: 148 WVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 186 YLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE-E 245
           +LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  T  S  +  E  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIH 267

Query: 246 DAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPV--AIFYTSFLGKANKAVK 305
           + + L+++WQ + Y  EK +      I   +T    GK K    + F + F G  +  V 
Sbjct: 268 ELVKLVNKWQNIAYMYEKELLLFTHFITRNITDN-QGKNKTTIHSYFSSIFHGGVDSLVD 327

Query: 306 ILKEKFPQLGLKKEECKEASWVESVVI---AANDFTVGEPVEALLNRSALIPPITSKKVK 365
           ++ + FP+LG+KK +CK+ SW+++++      N  T     E LL+RS            
Sbjct: 328 LMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSG----GRKAAFS 387

Query: 366 IKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLF 425
           IK DYVK+P+P+ A+  I  ++   +D+G    +F PYGG M EISES I F HRAG  +
Sbjct: 388 IKLDYVKKPIPETAMVTILEKL-YEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITY 447

Query: 426 KIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNI 485
           +I Y+  WE    +   +H+NWIR +Y++  P+VS++PR AY+NYRDLD+G  + +  N 
Sbjct: 448 EIWYIASWE--KQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPN- 507

Query: 486 VTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
             N+ QA  WG KY+G NFNRLV++KTKVDP NFFR+EQSIP
Sbjct: 508 --NYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIP 538

BLAST of CsaV3_1G037510 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 9.2e-138
Identity = 248/522 (47.51%), Postives = 351/522 (67.24%), Query Frame = 0

Query: 6   SDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILIITP 65
           ++  E FL+C   + P + + AKL+YTQ++  Y S+LN +I+N RF++  TPKP++IITP
Sbjct: 28  ANPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITP 87

Query: 66  SNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRRTA 125
            N+SHIQ  ++CSK  GLQIR RSGGHD EG+SY++ +PF++VDL N+ S+ +DV  +TA
Sbjct: 88  LNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTA 147

Query: 126 WVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVIDA 185
           WV++ ATLGE+YY I E +  L+FP G CPTV  GG+ SGGGYG L+R YGLAADN+IDA
Sbjct: 148 WVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 186 YLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE-E 245
           +LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  T  S  +  E  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIH 267

Query: 246 DAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPV--AIFYTSFLGKANKAVK 305
           + + L+++WQ + Y  EK +      I   +T    GK K    + F + F G  +  V 
Sbjct: 268 ELVKLVNKWQNIAYMYEKELLLFTHFITRNITDN-QGKNKTTIHSYFSSIFHGGVDSLVD 327

Query: 306 ILKEKFPQLGLKKEECKEASWVESVVI---AANDFTVGEPVEALLNRSALIPPITSKKVK 365
           ++ + FP+LG+KK +CK+ SW+++++      N  T     E LL+RS            
Sbjct: 328 LMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSG----GRKAAFS 387

Query: 366 IKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLF 425
           IK DYVK+P+P+ A+  I  ++   +D+G    +F PYGG M EISES I F HRAG ++
Sbjct: 388 IKLDYVKKPIPETAMVTILEKL-YEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMY 447

Query: 426 KIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNI 485
           +I Y+  WE    +   +H+NWIR +Y++  P+VS++PR AY+NYRDLD+G  + +  N 
Sbjct: 448 EIWYIASWE--KQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPN- 507

Query: 486 VTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
             N+ QA  WG KY+G NFNRLV++KTKVDP NFFR+EQSIP
Sbjct: 508 --NYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIP 538

BLAST of CsaV3_1G037510 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 3.5e-137
Identity = 253/526 (48.10%), Postives = 354/526 (67.30%), Query Frame = 0

Query: 6   SDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILIITP 65
           ++  E FL+C   + P++ +  K IYTQ++  Y SVLN +I+N RF++  TPKP++I+TP
Sbjct: 28  ANPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTP 87

Query: 66  SNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRRTA 125
           SN+SHIQA+++CSK  GLQIR RSGGHD EGLSY++ +PF +VDL N+ ++ VD+  +TA
Sbjct: 88  SNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTA 147

Query: 126 WVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVIDA 185
           WV++ ATLGE+YY I E +   +FPGG CPTV  GG+ SGGGYG L+R YGLAADN+IDA
Sbjct: 148 WVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 186 YLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE-E 245
           +LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+  T  S  +  E  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIH 267

Query: 246 DAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVD--GKVKPVAIFYTS--FLGKANKA 305
             + L ++WQ + YK +K++   +LT   +  +  D  GK K     Y S  FLG  +  
Sbjct: 268 GLVKLFNKWQNIAYKYDKDL---MLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSL 327

Query: 306 VKILKEKFPQLGLKKEECKEASWVESVVI---AANDFTVGEPVEALLNRSALIPPITSKK 365
           V ++ + FP+LG+KK +CKE SW+++ +      N  T     E LL+RSA       KK
Sbjct: 328 VDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDRSA------GKK 387

Query: 366 V--KIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRA 425
               IK DYVK+ +P+ A+  I  ++   +   G+ VL+ PYGG M EISES I F HRA
Sbjct: 388 TAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLY-PYGGIMDEISESAIPFPHRA 447

Query: 426 GNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDK 485
           G ++++ Y   WE    +   +H+NW+R +Y++  P+VS++PR AY+NYRDLD+G  + +
Sbjct: 448 GIMYELWYTATWE--KQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPE 507

Query: 486 YGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
             N   N+ QA  WG KY+G NFNRLV++KTK DP NFFR+EQSIP
Sbjct: 508 SPN---NYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIP 538

BLAST of CsaV3_1G037510 vs. ExPASy TrEMBL
Match: A0A5A7TYU2 (Cannabidiolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00260 PE=3 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 9.4e-210
Identity = 361/524 (68.89%), Postives = 432/524 (82.44%), Query Frame = 0

Query: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 63
           +S   HEEFL CLLHHSP S SI KL+YT  N+SYS VLN SIRN RFSTPNTPKP+LII
Sbjct: 27  SSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTSYSPVLNFSIRNLRFSTPNTPKPLLII 86

Query: 64  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 123
           TPSNISH+QAAVICSKS G+QIRIRSGGHDFEGLSYVAY PF+VVDLINL S+T++V++ 
Sbjct: 87  TPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGLSYVAYHPFVVVDLINLSSVTIEVEQS 146

Query: 124 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 183
           TAWVQS ATLGELYYRIAEKS TL FP G CPTV  GG+ SGGG+G LLRKYGLAADNVI
Sbjct: 147 TAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTVGVGGHFSGGGFGTLLRKYGLAADNVI 206

Query: 184 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 243
           DAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVT C+ SRT E
Sbjct: 207 DAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTISRTLE 266

Query: 244 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI 303
           E+AI LI QWQYV  KL++++  G+  +GG++ ++ D K+ P+A+F++ FLGKA++ + I
Sbjct: 267 EEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVAQGD-KINPIALFFSLFLGKADELMAI 326

Query: 304 LKEKFPQLGLKKEECKEASWVESVVIAANDFTV-GEPVEALLNRSALIPPITSKKVKIKS 363
           L + FPQLGL KEECKE SW+ESVV   N   +  +P+E LLNR+    P+ +  +K+KS
Sbjct: 327 LNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPLEVLLNRT----PLANGNIKMKS 386

Query: 364 DYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIA 423
           DYVKEP+ K  I+ +W R+  SQDI G N++F+PYGGRMS+ISESEI FSHRAGNL+KI 
Sbjct: 387 DYVKEPISKATIKEVWQRL-KSQDIEGANLVFIPYGGRMSQISESEIPFSHRAGNLYKIG 446

Query: 424 YLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTN 483
           YLTGW +P ++ E  HLNWIR+IYSYM PFV+KSPR+AYVNYRDLDIGSNS KYG   T+
Sbjct: 447 YLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAYVNYRDLDIGSNS-KYGK--TS 506

Query: 484 HDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQ 527
           + +A  WG KY+G+NF+RLV +K +VDPYNFFRHEQSIP+ L +
Sbjct: 507 YKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPVLLKR 541

BLAST of CsaV3_1G037510 vs. ExPASy TrEMBL
Match: A0A1S3BCV7 (cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488658 PE=3 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 9.4e-210
Identity = 361/524 (68.89%), Postives = 432/524 (82.44%), Query Frame = 0

Query: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 63
           +S   HEEFL CLLHHSP S SI KL+YT  N+SYS VLN SIRN RFSTPNTPKP+LII
Sbjct: 27  SSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTSYSPVLNFSIRNLRFSTPNTPKPLLII 86

Query: 64  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 123
           TPSNISH+QAAVICSKS G+QIRIRSGGHDFEGLSYVAY PF+VVDLINL S+T++V++ 
Sbjct: 87  TPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGLSYVAYHPFVVVDLINLSSVTIEVEQS 146

Query: 124 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 183
           TAWVQS ATLGELYYRIAEKS TL FP G CPTV  GG+ SGGG+G LLRKYGLAADNVI
Sbjct: 147 TAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTVGVGGHFSGGGFGTLLRKYGLAADNVI 206

Query: 184 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 243
           DAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVT C+ SRT E
Sbjct: 207 DAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTISRTLE 266

Query: 244 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI 303
           E+AI LI QWQYV  KL++++  G+  +GG++ ++ D K+ P+A+F++ FLGKA++ + I
Sbjct: 267 EEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVAQGD-KINPIALFFSLFLGKADELMAI 326

Query: 304 LKEKFPQLGLKKEECKEASWVESVVIAANDFTV-GEPVEALLNRSALIPPITSKKVKIKS 363
           L + FPQLGL KEECKE SW+ESVV   N   +  +P+E LLNR+    P+ +  +K+KS
Sbjct: 327 LNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPLEVLLNRT----PLANGNIKMKS 386

Query: 364 DYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIA 423
           DYVKEP+ K  I+ +W R+  SQDI G N++F+PYGGRMS+ISESEI FSHRAGNL+KI 
Sbjct: 387 DYVKEPISKATIKEVWQRL-KSQDIEGANLVFIPYGGRMSQISESEIPFSHRAGNLYKIG 446

Query: 424 YLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTN 483
           YLTGW +P ++ E  HLNWIR+IYSYM PFV+KSPR+AYVNYRDLDIGSNS KYG   T+
Sbjct: 447 YLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAYVNYRDLDIGSNS-KYGK--TS 506

Query: 484 HDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQ 527
           + +A  WG KY+G+NF+RLV +K +VDPYNFFRHEQSIP+ L +
Sbjct: 507 YKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPVLLKR 541

BLAST of CsaV3_1G037510 vs. ExPASy TrEMBL
Match: A0A1S3BDD9 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488665 PE=3 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 1.7e-206
Identity = 361/521 (69.29%), Postives = 425/521 (81.57%), Query Frame = 0

Query: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 63
           +SS KHEEFLQCLLHHSP +SSI KL++T  NSS+S++ N   RN RFSTPNTPKP+LII
Sbjct: 40  SSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSSFSTLFNFPTRNSRFSTPNTPKPLLII 99

Query: 64  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 123
           TPSNISHIQAAVICSKS GLQIRIRSGGHDFEGLSYVAY  FIVVDLINLRS+TVDV++ 
Sbjct: 100 TPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHRFIVVDLINLRSVTVDVEQS 159

Query: 124 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 183
           TAWVQS ATLGELYY+I +KS TL FP G CPTV  GG+ SGGGYG LLRKYG+AADNVI
Sbjct: 160 TAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVI 219

Query: 184 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 243
           DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG+VVAWKVKLVPVPA+VT CS+ R+ E
Sbjct: 220 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPASVTICSAKRSVE 279

Query: 244 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI 303
           E+AI LI +WQYV  KLE+++   +L  GG+++++  GK+ P+A+F+  FLG  N+ + I
Sbjct: 280 EEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSAQGGGKINPIALFFPLFLGNVNELMAI 339

Query: 304 LKEKFPQLGLKKEECKEASWVESVVIAANDFTV-GEPVEALLNRSALIPPITSKKVKIKS 363
           L + FP+LG  KEECKE SW+ES   AAN F +  +P+EALLNR+   P       K+KS
Sbjct: 340 LNKTFPELGFTKEECKEKSWIESAASAANRFQIEDQPLEALLNRT---PINHGSSFKLKS 399

Query: 364 DYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIA 423
           DYVKEPM K AIEGIW R+  SQDI G+ +  +PYGGRMS ISES I FSHRAG L++I 
Sbjct: 400 DYVKEPMTKTAIEGIWKRL-ESQDIEGVTLALIPYGGRMSRISESNIPFSHRAGILYQIG 459

Query: 424 YLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTN 483
           Y+ GW++  ++ E RHLNWIREIYSYM PFVSKSPR+AYVNYRDLDIGSN +KYG I  N
Sbjct: 460 YILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTAYVNYRDLDIGSN-NKYGKI--N 519

Query: 484 HDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIA 524
           + QA  +G KY+GNNFNRLV++KTKVDPYNFF HEQSIP++
Sbjct: 520 YKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPLS 553

BLAST of CsaV3_1G037510 vs. ExPASy TrEMBL
Match: A0A6J1KJP1 (berberine bridge enzyme-like 27 OS=Cucurbita maxima OX=3661 GN=LOC111495223 PE=3 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 2.4e-205
Identity = 356/530 (67.17%), Postives = 428/530 (80.75%), Query Frame = 0

Query: 5   SSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILIIT 64
           SS+++++FLQCLLHHSP + SI KL+YT  N+SYSS+LN SI+N RFSTP TPKP+ IIT
Sbjct: 26  SSNQNKDFLQCLLHHSPDTHSITKLVYTPINASYSSLLNFSIKNLRFSTPKTPKPLAIIT 85

Query: 65  PSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRRT 124
           PSNI HIQAA+ICSKS GLQIRIRSGGHD EGLSYVAY+PFIVVDLINL  + VDV++ T
Sbjct: 86  PSNIHHIQAAIICSKSHGLQIRIRSGGHDLEGLSYVAYVPFIVVDLINLSLVEVDVEKNT 145

Query: 125 AWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVID 184
           AWVQS A+LGELYYRIAEKS TL FP G CPTV  GG  SGGGYG+L+RKYGLAADNV+D
Sbjct: 146 AWVQSGASLGELYYRIAEKSRTLAFPAGLCPTVGVGGQFSGGGYGMLMRKYGLAADNVVD 205

Query: 185 AYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFEE 244
           AYLVDANGEF DRE MGE+LFWAIRGGGGGSFG+VVAWKVKLVPVPATVT C++S++  +
Sbjct: 206 AYLVDANGEFFDREGMGEELFWAIRGGGGGSFGVVVAWKVKLVPVPATVTVCATSKSLVD 265

Query: 245 DAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKIL 304
            AINLI +WQYV  KL++N+  G++  GG       GK  P+AIF++ FLGKA++ V IL
Sbjct: 266 GAINLIDKWQYVADKLDENLFLGIILAGG-------GKTNPLAIFFSLFLGKADELVTIL 325

Query: 305 KEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKSDY 364
              FP+LGL KE+C E SW+E+ +IAAN F  GE +EALLNRSALIPPI S + K KSDY
Sbjct: 326 NNPFPELGLSKEDCIETSWIEAALIAANGFQTGEVLEALLNRSALIPPINSGRSKTKSDY 385

Query: 365 VKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIAYL 424
           VKEPM + AIEG+WNR + SQ+I   +++F+PYGG+MSEISESEI F HRAGN ++I Y+
Sbjct: 386 VKEPMTRTAIEGMWNR-SKSQEIERASLVFIPYGGKMSEISESEIPFPHRAGNRYEIDYI 445

Query: 425 TGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNHD 484
            GW++  +  E RHLNWIRE+YSYM PFVSKSPR+ YVN RDLDIGSN D YG   T++ 
Sbjct: 446 VGWDEQGVHAEKRHLNWIRELYSYMTPFVSKSPRAVYVNCRDLDIGSN-DMYGR--TSYK 505

Query: 485 QASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQEDNLVKLV 535
           +A  WGLKY+GNNF+RLV +KT+VDPY+FFRHEQSIP  LS      K++
Sbjct: 506 RARVWGLKYFGNNFDRLVHVKTEVDPYDFFRHEQSIP-TLSPTKEFSKII 543

BLAST of CsaV3_1G037510 vs. ExPASy TrEMBL
Match: A0A6J1EMV2 (berberine bridge enzyme-like 27 OS=Cucurbita moschata OX=3662 GN=LOC111434009 PE=3 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 3.1e-205
Identity = 357/530 (67.36%), Postives = 429/530 (80.94%), Query Frame = 0

Query: 5   SSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILIIT 64
           SS+++++FLQCLLHHSP + SI KL+YT  N+SYSS+LN SI+N RFSTP TPKP+ IIT
Sbjct: 26  SSNQNKDFLQCLLHHSPDTHSITKLVYTPINASYSSLLNFSIKNLRFSTPKTPKPLAIIT 85

Query: 65  PSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRRT 124
           PSNI HIQAA+ICSKS GLQIRIRSGGHD EGLSYVA++PFIVVDLINL S+ VDV++ T
Sbjct: 86  PSNIPHIQAAIICSKSHGLQIRIRSGGHDLEGLSYVAHVPFIVVDLINLSSVEVDVEKNT 145

Query: 125 AWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVID 184
           AWVQS A+LGELYYRIAEKS TLTFP G CPTV  GG  SGGGYG+L+RKYGLAADNV+D
Sbjct: 146 AWVQSGASLGELYYRIAEKSRTLTFPAGLCPTVGVGGQFSGGGYGMLMRKYGLAADNVVD 205

Query: 185 AYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFEE 244
           AYLVD NGEF DRE MGE+LFWAIRGGGGGSFG+VVAWKVKLVPVPATVT C++S++  +
Sbjct: 206 AYLVDGNGEFFDREGMGEELFWAIRGGGGGSFGVVVAWKVKLVPVPATVTVCATSKSLVD 265

Query: 245 DAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKIL 304
            AI+LI +WQYV  KL++N+  G++  GG       GK  P+AIF++ FLGKA++ V IL
Sbjct: 266 GAISLIDKWQYVADKLDENLFLGIILAGG-------GKTNPLAIFFSLFLGKADELVTIL 325

Query: 305 KEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKSDY 364
              FP+LGL K +C E SW+E+ +IAAN F  GE +EALLNRSALIPPI S K K KSDY
Sbjct: 326 NNAFPELGLPKGDCIETSWIEAALIAANGFQTGEVLEALLNRSALIPPINSGKSKTKSDY 385

Query: 365 VKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIAYL 424
           VKEPM + AIEG+WNR + SQ+I   +++F+PYGG+MSEISESEI F HRAGN ++I Y+
Sbjct: 386 VKEPMTRTAIEGMWNR-SKSQEIERASLVFIPYGGKMSEISESEIPFPHRAGNRYEIDYI 445

Query: 425 TGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNHD 484
            GW++  +  E RHLNWIRE+YSYMAPFVSKSPR+AYVN RDLDIGSN D YG   T++ 
Sbjct: 446 VGWDEQGVHAEKRHLNWIRELYSYMAPFVSKSPRAAYVNCRDLDIGSN-DMYGR--TSYQ 505

Query: 485 QASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQEDNLVKLV 535
           +A  WGLKY+GNNF+RL  +KT+VDPY+FFRHEQSIP  LS      K+V
Sbjct: 506 RARVWGLKYFGNNFDRLAHVKTEVDPYDFFRHEQSIP-TLSPTQEFSKIV 543

BLAST of CsaV3_1G037510 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 486.1 bits (1250), Expect = 3.6e-137
Identity = 245/526 (46.58%), Postives = 346/526 (65.78%), Query Frame = 0

Query: 1   MTKASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPI 60
           +  A    HE+FL+CL +    ++   K+I+T  +SS+ S+L+ SI+N RFS   TPKP+
Sbjct: 20  LVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSETPKPV 79

Query: 61  LIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAY-LPFIVVDLINLRSITVD 120
            IITP   S +Q  + C++  G+ +R RS GH +EGLSY+AY  PF V+DL NLRSI++D
Sbjct: 80  SIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLRSISLD 139

Query: 121 VKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAA 180
           V  RT WVQ+ AT GELYY I + + +L FP G  PTV  GG  SGGGYG LLRKYGLAA
Sbjct: 140 VDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKYGLAA 199

Query: 181 DNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSS 240
           DN+IDA +VDA+G   DR++MGED FWAIRGGGG SFG++++WKVKLV VP+T+T     
Sbjct: 200 DNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITVFKVQ 259

Query: 241 RTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANK 300
           +T +++A+ +I +WQY   K+  ++         + T E   K    A+F   ++G  N 
Sbjct: 260 KTSKKEAVRIIKKWQYAADKVPDDLFI-------RTTLERSNKNAVHALFTGLYIGPVNN 319

Query: 301 AVKILKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVK 360
            + +++EKFP+LGL+KE C+E SW+ESV+  A DF  GE +  L NR       TS   K
Sbjct: 320 LLALMEEKFPELGLEKEGCEEMSWIESVLWFA-DFPKGESLGVLTNRER-----TSLSFK 379

Query: 361 IKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLF 420
            K D+V+EP+P+ AI+ IW R+   +   G  ++  P+GG+MSE++E E  F HR GNL+
Sbjct: 380 GKDDFVQEPIPEAAIQEIWRRLEAPEARLG-KIILTPFGGKMSEMAEYETPFPHRGGNLY 439

Query: 421 KIAYLTGW---EDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKY 480
           +I Y+  W   ED +     ++L W+  +Y +M P+VSKSPR AYVN++D+D+G      
Sbjct: 440 EIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGM---YL 499

Query: 481 GNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPI 523
           G   T +++  SWG+KY+ NNF RLV++KT+VDP +FF  EQSIP+
Sbjct: 500 GKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPL 528

BLAST of CsaV3_1G037510 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 483.4 bits (1243), Expect = 2.3e-136
Identity = 246/523 (47.04%), Postives = 344/523 (65.77%), Query Frame = 0

Query: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 63
           A    HE FL+CL H      S  ++I+T  + SY S+LN SI+N RF    TPKP+ II
Sbjct: 29  AQRTNHENFLKCLSHRINEDDS--RIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSII 88

Query: 64  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVA-YLPFIVVDLINLRSITVDVKR 123
           TP   + +Q+ + C++  G+ IR RSGGHD+EGLSY+A   PF+V+DL NLRSIT+DV  
Sbjct: 89  TPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDN 148

Query: 124 RTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNV 183
           RT WVQS AT+GELYY I + S +L FP G  PTV  GG   GGGYG L+RKYGL+ADNV
Sbjct: 149 RTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNV 208

Query: 184 IDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTF 243
           IDA++VDANG F DR+ MGED FWAIRGGGG SF +V++WK++L+ VP+ VT     +T 
Sbjct: 209 IDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTS 268

Query: 244 EEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVK 303
           E++A+++I++WQY+  K+  +     L I   +  E +      A F   +LG  +  + 
Sbjct: 269 EKEAVSIINKWQYIADKVPND-----LFIRAMLQKETE----VYASFPGLYLGPVSDLLA 328

Query: 304 ILKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKS 363
           ++K+KFP+LGL+   C+E SW+ESV+     F  GE +E L  R       TS+  K K 
Sbjct: 329 LMKDKFPELGLEIGNCREMSWIESVLW----FIKGESMEILAKRKR-----TSRSFKGKD 388

Query: 364 DYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIA 423
           D+++EP+PK AI+ +W R   + +     ++  P+GG+MSEI+++EI F HR GNL++I 
Sbjct: 389 DFIEEPIPKTAIQYLWRRF-EAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQ 448

Query: 424 YLTGW---EDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNI 483
           YL  W   ED +     ++L W+  +Y +M P+VSKSPR AYVN+RD+D+G       N+
Sbjct: 449 YLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGL--NM 508

Query: 484 VTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPI 523
            T +++A  WG+KY+ NNF+RLV++KT VDP +FF  EQSIPI
Sbjct: 509 KTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPI 528

BLAST of CsaV3_1G037510 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 481.1 bits (1237), Expect = 1.2e-135
Identity = 244/518 (47.10%), Postives = 343/518 (66.22%), Query Frame = 0

Query: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 63
           A+      FLQCL      S+ ++K+I+T N++S+SSVL  SI+N RFS P+ PKP+LI+
Sbjct: 29  ANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLIL 88

Query: 64  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 123
           TP   S +Q+AV C++  G+ IR RSGGHD+EGLSYV + PF+++DL NLRSITVDV  R
Sbjct: 89  TPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNR 148

Query: 124 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 183
           + WVQ+ AT+GELYY I +K+ TL FP G CPTV  GG+ SGGGYG LLRK+GLAAD+VI
Sbjct: 149 SVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVI 208

Query: 184 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 243
           DA +VDA G   +R  MGED FWAIRGGGG SF +V++WK+ L+ VP+TVT  + ++  E
Sbjct: 209 DARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSE 268

Query: 244 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI 303
           + A+ +IH+WQ+V  K+  ++   V      M       V+  A F   +LG     +K+
Sbjct: 269 QSALKIIHRWQFVADKVSDDLFIRV------MLQRYKNMVR--ASFPGLYLGSVKNLLKM 328

Query: 304 LKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKSD 363
           + ++FP+LGL++++C E SW+ESV+  A      EP+  L  R+       S   K KSD
Sbjct: 329 VNKEFPELGLEEDDCTEMSWIESVIWFAE--LGEEPINVLTKRTR-----ASLAFKAKSD 388

Query: 364 YVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIAY 423
           +V+EPMPK AI  +W R+    +     ++F P+GG+MSEI++ E  F HR GN+++I Y
Sbjct: 389 FVQEPMPKTAISKLWRRL-QEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQY 448

Query: 424 LTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNH 483
           L  W     DV+ +++ W+  +Y  M+ FV+KSPR AY+N RDLD+G      G   + +
Sbjct: 449 LNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGM---YVGVKRSKY 508

Query: 484 DQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
           ++  SWG+KY+ NNF RLV++KT VDP +FF  EQSIP
Sbjct: 509 EEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of CsaV3_1G037510 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 475.3 bits (1222), Expect = 6.4e-134
Identity = 240/522 (45.98%), Postives = 341/522 (65.33%), Query Frame = 0

Query: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYT--QNNSSYSSVLNLSIRNHRFSTPNTPKPIL 63
           +SS   ++F++CL+ +S  S  I    ++  QN + +   L  + +N R+ TP+ PKP+ 
Sbjct: 25  SSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQNLRYLTPSNPKPVF 84

Query: 64  IITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVA--YLPFIVVDLINLRSITVD 123
           I  P   +H+QAAV+C+K   L +R+RSGGHD+EGLS+VA    PF++VDL  LR + VD
Sbjct: 85  IFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFVIVDLSKLRQVDVD 144

Query: 124 VKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAA 183
           +   +AW  + AT+GE+YYRI EKS T  FP G C ++  GG+L GG YG ++RK+GL A
Sbjct: 145 LDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGSMMRKFGLGA 204

Query: 184 DNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSS 243
           DNV+DA +VDANG+  DR +MGED+FWAIRGGGGGSFG+++AWK+KLVPVPATVT  + +
Sbjct: 205 DNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPATVTVFTVT 264

Query: 244 RTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANK 303
           +T E+D   ++++W+ +  KL+ ++   V+      T++   +   ++ +   FLG +N+
Sbjct: 265 KTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISMS-YQAQFLGDSNR 324

Query: 304 AVKILKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVK 363
            ++++++ FP+LGL K++C E SW++SV+  A  F      EALL   +L         K
Sbjct: 325 LLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAG-FPNSAAPEALLAGKSLF----KNHFK 384

Query: 364 IKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLF 423
            KSD+VKEP+P   +EG+W R            ++ PYGG MS ISESEI F HR G LF
Sbjct: 385 AKSDFVKEPIPVEGLEGLWERFLEED---SPLTIWNPYGGMMSRISESEIPFPHRNGTLF 444

Query: 424 KIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNI 483
           KI +L+ W+D  +  E RH+ WIRE+YSYM  +VSK+PR AYVNYRDLD+G+N       
Sbjct: 445 KIQWLSTWQDGKVS-EERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNE------ 504

Query: 484 VTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
                 A  WG KYY  NF RLV+IK + DP NFFRHEQS+P
Sbjct: 505 --GETDAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of CsaV3_1G037510 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 474.2 bits (1219), Expect = 1.4e-133
Identity = 252/527 (47.82%), Postives = 341/527 (64.71%), Query Frame = 0

Query: 2   TKASSDKHEEFLQCLLHHSPHSSSIAKLIYT--QNNSSYSSVLNLSIRNHRFSTPNTPKP 61
           T +S+   ++F+ C+  ++  S  + K ++T  +N S ++ VL  + +N +F   + PKP
Sbjct: 28  TSSSASIQDQFINCVKRNTHVSFPLEKTLFTPAKNVSLFNQVLESTAQNLQFLAKSMPKP 87

Query: 62  ILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYL--PFIVVDLINLRSIT 121
             I  P + S +QA++ICSK  G+  R+RSGGHDFE LSYV+ +  PFI++DL  L+ I 
Sbjct: 88  GFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIEKPFILLDLSKLKQIN 147

Query: 122 VDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGL 181
           VD++  +AWVQ  ATLGELYYRIAEKS    FP G C +V  GGY++GGGYG L+RKYGL
Sbjct: 148 VDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYMTGGGYGTLMRKYGL 207

Query: 182 AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCS 241
           A DNV+D  +VDANG+  DR +MGEDLFWAIRGGGG SFGIV+AWK+KLVPVP TVT  +
Sbjct: 208 AGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLVPVPKTVTVFT 267

Query: 242 SSRTFEEDA-INLIHQWQYVGYKLEKNI-AHGVLTIGGQMTSEVDGKVKPVAIFYTSFLG 301
            ++T E+DA +  I +WQ +  K+ + I    VL   G      DG       +   FLG
Sbjct: 268 VTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAAGN-----DGNKTVTMTYLGQFLG 327

Query: 302 KANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITS 361
           +    +K++++ FP+LGL +++C E SW+E+ +     F  G P+E LL    L  P+  
Sbjct: 328 EKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGG-FPTGSPIEILLQ---LKSPLGK 387

Query: 362 KKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVL-FVPYGGRMSEISESEISFSHR 421
              K  SD+VKEP+P + ++GI+ R+      G    L + PYGG MS+I ES I F HR
Sbjct: 388 DYFKATSDFVKEPIPVIGLKGIFKRLIE----GNTTFLNWTPYGGMMSKIPESAIPFPHR 447

Query: 422 AGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSD 481
            G LFKI Y   W + +    +R +NWI+EIY+YMAP+VS +PR AYVNYRDLD G N +
Sbjct: 448 NGTLFKILYYANWLE-NDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQNKN 507

Query: 482 KYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
              N   N  +A  WG KY+  NF+RLV+IKTKVDP NFFRHEQSIP
Sbjct: 508 ---NAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIP 537

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAE8653320.10.0e+00100.00hypothetical protein Csa_023331 [Cucumis sativus][more]
XP_004135739.18.2e-310100.00berberine bridge enzyme-like 28 [Cucumis sativus][more]
XP_011659204.29.3e-21269.90berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653317.1 hypothetical pro... [more]
XP_038895343.13.9e-21070.25cannabidiolic acid synthase-like [Benincasa hispida][more]
XP_038895262.11.9e-20969.22tetrahydrocannabinolic acid synthase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A6P6V93.4e-14048.08Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1[more]
Q8GTB67.6e-14048.85Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV... [more]
A6P6W17.1e-13847.51Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=... [more]
A6P6W09.2e-13847.51Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=... [more]
Q33DQ23.5e-13748.10Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7TYU29.4e-21068.89Cannabidiolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3BCV79.4e-21068.89cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488658 PE=3 SV... [more]
A0A1S3BDD91.7e-20669.29tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10348866... [more]
A0A6J1KJP12.4e-20567.17berberine bridge enzyme-like 27 OS=Cucurbita maxima OX=3661 GN=LOC111495223 PE=3... [more]
A0A6J1EMV23.1e-20567.36berberine bridge enzyme-like 27 OS=Cucurbita moschata OX=3662 GN=LOC111434009 PE... [more]
Match NameE-valueIdentityDescription
AT5G44440.13.6e-13746.58FAD-binding Berberine family protein [more]
AT5G44410.12.3e-13647.04FAD-binding Berberine family protein [more]
AT4G20820.11.2e-13547.10FAD-binding Berberine family protein [more]
AT2G34790.16.4e-13445.98FAD-binding Berberine family protein [more]
AT5G44380.11.4e-13347.82FAD-binding Berberine family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012951Berberine/berberine-likePFAMPF08031BBEcoord: 460..521
e-value: 6.7E-22
score: 77.3
NoneNo IPR availableGENE3D3.40.462.20coord: 232..456
e-value: 5.3E-149
score: 498.7
NoneNo IPR availablePANTHERPTHR32448:SF113BERBERINE BRIDGE ENZYME-LIKE 18coord: 7..522
NoneNo IPR availablePANTHERPTHR32448OS08G0158400 PROTEINcoord: 7..522
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 59..195
e-value: 3.3E-25
score: 88.5
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 117..521
e-value: 5.3E-149
score: 498.7
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 6..113
e-value: 3.0E-44
score: 151.2
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 55..229
score: 19.158665
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 9..230

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_1G037510.1CsaV3_1G037510.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0071949 FAD binding
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity