CsaV3_1G037100 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_1G037100 vs. NCBI nr
Match: XP_004135725.2 (berberine bridge enzyme-like 15 [Cucumis sativus] >KAE8653307.1 hypothetical protein Csa_023228 [Cucumis sativus])

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 546/546 (100.00%), Postives = 546/546 (100.00%), Query Frame = 0

Query: 1   MMGIPQCRIRHYLFPLFYLSFICLSSSSHSLQADSFVECFNSTSYYYSPQSIPISKVVFT 60
           MMGIPQCRIRHYLFPLFYLSFICLSSSSHSLQADSFVECFNSTSYYYSPQSIPISKVVFT
Sbjct: 1   MMGIPQCRIRHYLFPLFYLSFICLSSSSHSLQADSFVECFNSTSYYYSPQSIPISKVVFT 60

Query: 61  NKSAIFSSLLQSSIKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGH 120
           NKSAIFSSLLQSSIKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGH
Sbjct: 61  NKSAIFSSLLQSSIKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGH 120

Query: 121 DYEGISYVSSDQSQFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFP 180
           DYEGISYVSSDQSQFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFP
Sbjct: 121 DYEGISYVSSDQSQFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFP 180

Query: 181 AGTCPTVGMGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRG 240
           AGTCPTVGMGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRG
Sbjct: 181 AGTCPTVGMGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRG 240

Query: 241 GGGASFGVILSWKLKLVYVPSNVTVFLVQKTLEQGAIHLFQKWQTIAHKLHEDLFLHVTI 300
           GGGASFGVILSWKLKLVYVPSNVTVFLVQKTLEQGAIHLFQKWQTIAHKLHEDLFLHVTI
Sbjct: 241 GGGASFGVILSWKLKLVYVPSNVTVFLVQKTLEQGAIHLFQKWQTIAHKLHEDLFLHVTI 300

Query: 301 GVIDEQNKTPNMSSKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVL 360
           GVIDEQNKTPNMSSKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVL
Sbjct: 301 GVIDEQNKTPNMSSKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVL 360

Query: 361 YFAGISIEAPPEILLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILS 420
           YFAGISIEAPPEILLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILS
Sbjct: 361 YFAGISIEAPPEILLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILS 420

Query: 421 PYGGKMRQISDLETPFPHRKGNTFGIQYLVTWENANETYRHLSWIREVYDYMEPYVSKYP 480
           PYGGKMRQISDLETPFPHRKGNTFGIQYLVTWENANETYRHLSWIREVYDYMEPYVSKYP
Sbjct: 421 PYGGKMRQISDLETPFPHRKGNTFGIQYLVTWENANETYRHLSWIREVYDYMEPYVSKYP 480

Query: 481 RAAYLNYRDLDLGRNCGRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIP 540
           RAAYLNYRDLDLGRNCGRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIP
Sbjct: 481 RAAYLNYRDLDLGRNCGRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIP 540

Query: 541 TSIYHS 547
           TSIYHS
Sbjct: 541 TSIYHS 546

BLAST of CsaV3_1G037100 vs. NCBI nr
Match: XP_008446963.1 (PREDICTED: flavin-dependent oxidoreductase FOX5-like [Cucumis melo])

HSP 1 Score: 1029.2 bits (2660), Expect = 1.3e-296
Identity = 500/546 (91.58%), Postives = 524/546 (95.97%), Query Frame = 0

Query: 2   MGIPQCRIRHYLFPLFYLSFICLSSSSHSLQADSFVECFNSTSYYYSPQSIPISKVVFTN 61
           MGIPQCRIRHYLF LFYL FI LSSSS+SLQA+SF+ECFNSTSYYYSPQSIPISKVVFT+
Sbjct: 1   MGIPQCRIRHYLFLLFYLPFIWLSSSSYSLQAESFLECFNSTSYYYSPQSIPISKVVFTS 60

Query: 62  K-SAIFSSLLQSSIKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGH 121
           K SA+FSSLLQSSIKNLRF NTSL KPLFLVTPFHQSHVQ+AIVCANKKG QIR+RSGGH
Sbjct: 61  KSSALFSSLLQSSIKNLRFLNTSLQKPLFLVTPFHQSHVQSAIVCANKKGLQIRVRSGGH 120

Query: 122 DYEGISYVSSDQSQFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFP 181
           DYEGISYVSSDQSQFILLD  N+RSIDIDMKTETA VEAGATLGELYYRIAEKSP HGFP
Sbjct: 121 DYEGISYVSSDQSQFILLDFINFRSIDIDMKTETAIVEAGATLGELYYRIAEKSPIHGFP 180

Query: 182 AGTCPTVGMGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRG 241
           AG+CPTVGMGGHV GGGFGTLFRKYGLAADNVIDAKIVD NGRIMDRNSMGEDLFWAIRG
Sbjct: 181 AGSCPTVGMGGHVGGGGFGTLFRKYGLAADNVIDAKIVDVNGRIMDRNSMGEDLFWAIRG 240

Query: 242 GGGASFGVILSWKLKLVYVPSNVTVFLVQKTLEQGAIHLFQKWQTIAHKLHEDLFLHVTI 301
           GGGASFGVILSWKLKLVYVPSNVTVF+VQKTLEQGAIHLFQKWQ IAHKLHEDLFLHVT+
Sbjct: 241 GGGASFGVILSWKLKLVYVPSNVTVFVVQKTLEQGAIHLFQKWQRIAHKLHEDLFLHVTM 300

Query: 302 GVIDEQNKTPNMSSKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVL 361
           GVIDEQNKTPNMSSKTILISFVSLFLG +ERLIPLMNSHFPELGLERNNCTEMSWIQSVL
Sbjct: 301 GVIDEQNKTPNMSSKTILISFVSLFLGRIERLIPLMNSHFPELGLERNNCTEMSWIQSVL 360

Query: 362 YFAGISIEAPPEILLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILS 421
           +FAGISIE PP++LLKRPP S +LFFKAKSDFVISPIPQIG EGLWTKMLEE AS+LIL+
Sbjct: 361 FFAGISIETPPKVLLKRPPTSKMLFFKAKSDFVISPIPQIGFEGLWTKMLEEEASYLILT 420

Query: 422 PYGGKMRQISDLETPFPHRKGNTFGIQYLVTWENANETYRHLSWIREVYDYMEPYVSKYP 481
           PYGGKMRQISD +TPFPHRKGN FGIQY+VTWENANETYRHLSWIREVYDYMEPYVSK+P
Sbjct: 421 PYGGKMRQISDFDTPFPHRKGNIFGIQYMVTWENANETYRHLSWIREVYDYMEPYVSKHP 480

Query: 482 RAAYLNYRDLDLGRNCGRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIP 541
           RAAYLNYRDLDLGRNCGRNTSYEEAKVWGLKY+K+NFDRLVRVKTKVDPLNFFWNEQSIP
Sbjct: 481 RAAYLNYRDLDLGRNCGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKVDPLNFFWNEQSIP 540

Query: 542 TSIYHS 547
           TS+YHS
Sbjct: 541 TSVYHS 546

BLAST of CsaV3_1G037100 vs. NCBI nr
Match: XP_022139883.1 (berberine bridge enzyme-like 13 [Momordica charantia])

HSP 1 Score: 826.2 bits (2133), Expect = 1.6e-235
Identity = 416/550 (75.64%), Postives = 469/550 (85.27%), Query Frame = 0

Query: 1   MMGIPQCRIRHYLFPLFYLSFICLSSSSHSLQADSFVECFNSTSYYYSPQS---IPISKV 60
           MMGI Q   R +LF LF++  I   SSS   Q  SF  CFNSTSYYYS  S   IPIS+V
Sbjct: 1   MMGILQ-YWRAHLFFLFFVVSIFFPSSSDYSQPQSFQXCFNSTSYYYSSHSIPIIPISEV 60

Query: 61  VFTNKSAIFSSLLQSSIKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRS 120
           +FTNKSA FSS+LQ SI+N RFFNTS PKPLFLVTPFH+ HVQAAIVCANKKG Q+R+RS
Sbjct: 61  LFTNKSASFSSVLQFSIRNFRFFNTSSPKPLFLVTPFHEFHVQAAIVCANKKGLQVRVRS 120

Query: 121 GGHDYEGISYVSSDQSQFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTH 180
           GGHDYEG+SY SS Q+ FI++DL + R+IDID+KTETA+VE GATLGELYYRIAEKSP H
Sbjct: 121 GGHDYEGLSYTSS-QAPFIVIDLIHLRAIDIDIKTETASVETGATLGELYYRIAEKSPIH 180

Query: 181 GFPAGTCPTVGMGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWA 240
           GFPAG+CPTVG+GGHVSGGGFGTLFRKYGLAADN+IDAKIVDF GRIMDRNSMGEDLFWA
Sbjct: 181 GFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNIIDAKIVDFKGRIMDRNSMGEDLFWA 240

Query: 241 IRGGGGASFGVILSWKLKLVYVPSNVTVFLVQKTLEQGAIHLFQKWQTIAHKLHEDLFLH 300
           IRGGG ASFGVILSWKLKLV VPS VTVFLVQKTLEQGA  LFQKWQTIA KL E LFLH
Sbjct: 241 IRGGGXASFGVILSWKLKLVSVPSKVTVFLVQKTLEQGATQLFQKWQTIADKLDEHLFLH 300

Query: 301 VTIGVIDEQNKTP-NMSSKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWI 360
           V IGV + QNK+P   S+KTIL+SF SLFLGP+++L  +M++HFPELGL+R N TEMSWI
Sbjct: 301 VIIGVTENQNKSPKEPSTKTILLSFESLFLGPIDKLSLIMSTHFPELGLQRENYTEMSWI 360

Query: 361 QSVLYFAGISIEAPPEILLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASF 420
           QSVL+F G+S EAP EILL+RPP+S++ FFKAKSD+V SPIPQ GLE LW+K+LEE AS 
Sbjct: 361 QSVLFFTGLSTEAPLEILLERPPLSDI-FFKAKSDYVTSPIPQTGLEALWSKVLEEEASL 420

Query: 421 LILSPYGGKMRQISDLETPFPHRKGNTFGIQYLVTWENANETYRHLSWIREVYDYMEPYV 480
           LIL+PYGGKM QIS+ ETPFPHR+G  FGIQY+VTWEN NET +HLSW+REVY  ME YV
Sbjct: 421 LILTPYGGKMSQISESETPFPHRRGIIFGIQYMVTWENGNETEKHLSWMREVYTCMELYV 480

Query: 481 SKYPRAAYLNYRDLDLGRNCGRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNE 540
           SK PR+AYL+YRDLDLGRN GRN SYEEAKVWGLKYFK NF+RLVRVKTKVDP N FWNE
Sbjct: 481 SKCPRSAYLSYRDLDLGRNYGRNRSYEEAKVWGLKYFKGNFERLVRVKTKVDPSNLFWNE 540

Query: 541 QSIPTSIYHS 547
           QSIP +++HS
Sbjct: 541 QSIPPTLHHS 547

BLAST of CsaV3_1G037100 vs. NCBI nr
Match: XP_038895849.1 (berberine bridge enzyme-like 8 [Benincasa hispida])

HSP 1 Score: 818.9 bits (2114), Expect = 2.6e-233
Identity = 415/541 (76.71%), Postives = 465/541 (85.95%), Query Frame = 0

Query: 7   CRIRHYLFPLFYLSFICLSSSSHSLQADSFVECFNSTSYYYSPQSIPISKVVFTNKSAIF 66
           C  R +LF LF++S I L SSS SLQ + F+ECFNSTS  YS  SIPIS+VVFTN+SA F
Sbjct: 3   CLRRVHLFLLFFVSLIWLCSSSDSLQ-ERFLECFNSTS--YSKHSIPISEVVFTNESASF 62

Query: 67  SSLLQSSIKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGHDYEGIS 126
           SSLLQ SI+NLRF  T++PKPLFLVTPFH+SHVQAAIVCA KKG Q+R+RSGGHDYEG+S
Sbjct: 63  SSLLQLSIRNLRFLTTTMPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLS 122

Query: 127 YVSSDQSQFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFPAGTCPT 186
           YVSS Q+ FI++DL N  SI ID++TET +VE GATLGELYY+IAEKSP HGFPAG+CPT
Sbjct: 123 YVSS-QAPFIVIDLINLHSITIDIETETTSVETGATLGELYYKIAEKSPIHGFPAGSCPT 182

Query: 187 VGMGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASF 246
           VG+GGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASF
Sbjct: 183 VGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASF 242

Query: 247 GVILSWKLKLVYVPSNVTVFLVQKTLEQGAIHLFQKWQTIAHKLHEDLFLHVTIGVIDEQ 306
           GVILSWKLKLV VPS VTVF VQ+TLEQGA HLFQKWQTIAHKL +D+FLHVT  VI   
Sbjct: 243 GVILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQTIAHKLDQDIFLHVTTKVI--- 302

Query: 307 NKTPNMSSKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVLYFAGIS 366
             T   S KTI +SF SLFLGP+ERLIP+M +HF ELGL+RN+CTEMSWIQSVL+FA  S
Sbjct: 303 --TNFSSKKTIRLSFTSLFLGPIERLIPIMKTHFSELGLKRNDCTEMSWIQSVLFFADFS 362

Query: 367 IEAPPEILLKR--PPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILSPYGG 426
           IE P E+L++R  PPIS+  FF AKSD+V SPI Q GLEGLWTK+LEE  S LIL+PYGG
Sbjct: 363 IETPLEVLMERSSPPISDA-FFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGG 422

Query: 427 KMRQISDLETPFPHRKGNTFGIQYLVTWENANETYRHLSWIREVYDYMEPYVSKYPRAAY 486
           KM QIS+ +TPFPHR+G+ FGIQYL TW+NANET +HLSWIREVY YMEPYVSK PRAAY
Sbjct: 423 KMSQISESQTPFPHRRGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAY 482

Query: 487 LNYRDLDLGRNCGRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIPTSIY 546
           LNYRDLDLGRN  +NTSYEEAKVWGLKYF +NF+RLVRVKTKVDPLNFFWNEQSIP  +Y
Sbjct: 483 LNYRDLDLGRNYRKNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPLNFFWNEQSIPL-LY 532

BLAST of CsaV3_1G037100 vs. NCBI nr
Match: KAG7019453.1 (Berberine bridge enzyme-like 15, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 810.4 bits (2092), Expect = 9.1e-231
Identity = 409/533 (76.74%), Postives = 460/533 (86.30%), Query Frame = 0

Query: 16  LFYLSFICLSSS--SHSLQADSFVECFNSTSYYYSPQSIPISKVVFTNKSAIFSSLLQSS 75
           LFYL  I LSSS  SH+LQ  SFVECF+S S   SPQSIPISKVVFTN+SA FSSLL  S
Sbjct: 5   LFYLPLIWLSSSSPSHALQ-QSFVECFSSHS---SPQSIPISKVVFTNESASFSSLLHQS 64

Query: 76  IKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGHDYEGISYVSSDQS 135
           I+NLRF NTS  KPLFLVTPFH+SHVQAA+VCA KKG Q+R+RSGGHDYEG+SYVSS QS
Sbjct: 65  IRNLRFLNTS-SKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSS-QS 124

Query: 136 QFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFPAGTCPTVGMGGHV 195
           QFILLDLSN  SIDID++TETA+VE GATLGELYYRIAEKS  HGFPAG+CPTVG+GGH+
Sbjct: 125 QFILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHI 184

Query: 196 SGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWK 255
           SGGGFGTLFRK+GLAADNVIDAKIVDFNGRIMDRN+MGE+LFWAIRGGGGASFGVILSWK
Sbjct: 185 SGGGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWK 244

Query: 256 LKLVYVPSNVTVFLVQKTLEQGAIHLFQKWQTIAHKLHEDLFLHVTIGVIDEQNKTPNMS 315
           LKLV +P  +T+F VQKT +QGAI LFQKWQ IA+KL EDLFLHVTIGV           
Sbjct: 245 LKLVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGV----------Q 304

Query: 316 SKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVLYFAGISIEAPPEI 375
            +TI +SFV+LFLGP+E+LIPLMN+ FPELGL+R N TEM+WIQS+L+FAG  I+ PP+I
Sbjct: 305 EQTISLSFVALFLGPIEKLIPLMNTQFPELGLQRENYTEMNWIQSILFFAGFPIQTPPQI 364

Query: 376 LLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILSPYGGKMRQISDLE 435
           LLK+  +SN  FFKAKSDFV  PIPQ GLEGLW K+LEE  S+LIL+PYGGKM QIS+LE
Sbjct: 365 LLKK-SLSNA-FFKAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELE 424

Query: 436 TPFPHRKGNTFGIQYLVTWENANETYRHLSWIREVYDYMEPYVSKYPRAAYLNYRDLDLG 495
           TPFPHRKGN FGIQY+V+WEN +E+Y+HLSWIREVY YMEPYVSK PR AYLNYRDLDLG
Sbjct: 425 TPFPHRKGNIFGIQYMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLG 484

Query: 496 RNCGRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIPTSIYHS 547
           RN GRN  YEEAKVWGLKY+++NF+RLVRVKTKVDP NFFWNEQSIPTS+Y S
Sbjct: 485 RNYGRNVRYEEAKVWGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPTSLYRS 519

BLAST of CsaV3_1G037100 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 1.3e-155
Identity = 278/531 (52.35%), Postives = 366/531 (68.93%), Query Frame = 0

Query: 16  LFYLSFICLSSSSHSLQADSFVECFNSTSYYYSPQSIPISKVVFT--NKSAIFSSLLQSS 75
           L  L  I +  SS +LQ D FV+C        S  S PI+   F+    + +F   L+S+
Sbjct: 14  LVTLLLISVPLSSSTLQQD-FVKCLVDN----SDVSFPITASFFSPDQNATLFKEELEST 73

Query: 76  IKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGHDYEGISYVSSDQS 135
            +NLR+   S PKP+F+  P +++HVQAA+VCA K    +RLRSGGHDYEG+S+V+ D++
Sbjct: 74  AQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDET 133

Query: 136 QFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFPAGTCPTVGMGGHV 195
            F+++DLS  R +D+D+ + +A   AGAT+GE+YYRI EKS THGFPAG C ++G+GGH+
Sbjct: 134 PFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHL 193

Query: 196 SGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWK 255
            GG +G++ RK+GL ADNV+DA+IVD NG+I+DR +MGED+FWAIRGGGG SFGVIL+WK
Sbjct: 194 VGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWK 253

Query: 256 LKLVYVPSNVTVFLVQKTLEQGAIHLFQKWQTIAHKLHEDLFLHVTIGVIDEQNKTPNMS 315
           +KLV VP+ VTVF V KTLEQ    +  KW+ IA KL +DLF+ V   +I   +KT    
Sbjct: 254 IKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRV---IISPASKTTKPG 313

Query: 316 SKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVLYFAGISIEAPPEI 375
           ++TI +S+ + FLG   RL+ +M   FPELGL + +CTEMSWI+SV+Y AG    A PE 
Sbjct: 314 NRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEA 373

Query: 376 LLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILSPYGGKMRQISDLE 435
           LL    +     FKAKSDFV  PIP  GLEGLW + LEE +   I +PYGG M +IS+ E
Sbjct: 374 LLAGKSLFK-NHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESE 433

Query: 436 TPFPHRKGNTFGIQYLVTWENAN-ETYRHLSWIREVYDYMEPYVSKYPRAAYLNYRDLDL 495
            PFPHR G  F IQ+L TW++      RH+ WIRE+Y YME YVSK PR AY+NYRDLDL
Sbjct: 434 IPFPHRNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDL 493

Query: 496 GRNCGRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIPTSI 544
           G N G      +A+ WG KY+K NF+RLV++K + DP NFF +EQS+PT I
Sbjct: 494 GTNEGET----DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPTKI 531

BLAST of CsaV3_1G037100 vs. ExPASy Swiss-Prot
Match: Q9FKU9 (Berberine bridge enzyme-like 25 OS=Arabidopsis thaliana OX=3702 GN=At5g44390 PE=2 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 4.2e-154
Identity = 279/537 (51.96%), Postives = 374/537 (69.65%), Query Frame = 0

Query: 13  LFPLFYLSFICLSSSSHSLQADSFVECFNSTSYYYSPQSIPISKVVFT--NKSAIFSSLL 72
           +F L++  +    +SS SLQ D F++C     Y  +     + K  FT    ++IF+ +L
Sbjct: 15  VFALYFSFYTITLTSSTSLQ-DDFIKCL----YRNTNVRFTLDKTFFTPERNASIFTEVL 74

Query: 73  QSSIKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGHDYEGISYVSS 132
           +S+ +N R+   ++PKP F+  P H+SHVQA+++C+ K     R+RSGGHDYEG+SYVS 
Sbjct: 75  ESTAQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQ 134

Query: 133 DQSQFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFPAGTCPTVGMG 192
            +  F+L+DLS  R I++D+K  +A VEAGAT+GELYYRIAEKS  HGFPAG  P++G+G
Sbjct: 135 IEKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIG 194

Query: 193 GHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVIL 252
           GH++GG +G+L RKYGLAADNV+DAKIVD NG+++DR SMGEDLFWAIRGG G SFG+IL
Sbjct: 195 GHITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIIL 254

Query: 253 SWKLKLVYVPSNVTVFLVQKTLEQG-AIHLFQKWQTIAHKLHEDLFLHVTIGVIDEQNKT 312
           SWK+KLV VP  +TVF V KT EQ  +  +  KWQ IA  L ++LFL V   V      +
Sbjct: 255 SWKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTV------S 314

Query: 313 PNMSSKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVLYFAGISIEA 372
            N ++KT+ ++++  FLG    L+ +M   FPELGL + +C EMSWI S++Y +G     
Sbjct: 315 GNKANKTVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNP 374

Query: 373 PP--EILLK-RPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILSPYGGKM 432
           PP  EILL+ + PI  V +FK KSDF   PIP +GLEG++ K+LEE A+ +I +PYGGKM
Sbjct: 375 PPPIEILLQAKSPIGKV-YFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGGKM 434

Query: 433 RQISDLETPFPHRKGNTFGIQYLVTWENANE-TYRHLSWIREVYDYMEPYVSKYPRAAYL 492
            +I + E PFPHR G  F IQY  +W ++ +   R   WIRE+Y YM PYVS  PR AY+
Sbjct: 435 DKIPESEIPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAYV 494

Query: 493 NYRDLDLGRNCGRNTS-YEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIPT 542
           NYRDLDLG+N   + S + EAK+WG  YFK+NF+RLVR+K+KVDP NFF +EQSIPT
Sbjct: 495 NYRDLDLGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPT 539

BLAST of CsaV3_1G037100 vs. ExPASy Swiss-Prot
Match: Q9FKV0 (Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=2 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 5.2e-152
Identity = 271/536 (50.56%), Postives = 368/536 (68.66%), Query Frame = 0

Query: 16  LFYLSFICL--SSSSHSLQADSFVECFNSTSYYYSPQSIPISKVVFT--NKSAIFSSLLQ 75
           + Y SF C+  +SSS S+Q D F+ C    ++     S P+ K +FT     ++F+ +L+
Sbjct: 17  VLYFSFYCITPTSSSASIQ-DQFINCVKRNTHV----SFPLEKTLFTPAKNVSLFNQVLE 76

Query: 76  SSIKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGHDYEGISYVSSD 135
           S+ +NL+F   S+PKP F+  P HQS VQA+I+C+ K G   R+RSGGHD+E +SYVS  
Sbjct: 77  STAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRI 136

Query: 136 QSQFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFPAGTCPTVGMGG 195
           +  FILLDLS  + I++D+++ +A V+ GATLGELYYRIAEKS  HGFPAG C +VG+GG
Sbjct: 137 EKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGG 196

Query: 196 HVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILS 255
           +++GGG+GTL RKYGLA DNV+D K+VD NG+++DR +MGEDLFWAIRGGGGASFG++L+
Sbjct: 197 YMTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLA 256

Query: 256 WKLKLVYVPSNVTVFLVQKTLEQGA-IHLFQKWQTIAHKLHEDLFLHVTIGVIDEQNKTP 315
           WK+KLV VP  VTVF V KTLEQ A +    KWQ I+ K+ E++ + V +          
Sbjct: 257 WKIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVL------RAAG 316

Query: 316 NMSSKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVLYFAGISIEAP 375
           N  +KT+ ++++  FLG    L+ +M   FPELGL + +CTEMSWI++ L+  G    +P
Sbjct: 317 NDGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSP 376

Query: 376 PEILLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILSPYGGKMRQIS 435
            EILL+        +FKA SDFV  PIP IGL+G++ +++E   +FL  +PYGG M +I 
Sbjct: 377 IEILLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTFLNWTPYGGMMSKIP 436

Query: 436 DLETPFPHRKGNTFGIQYLVTW-ENANETYRHLSWIREVYDYMEPYVSKYPRAAYLNYRD 495
           +   PFPHR G  F I Y   W EN   + R ++WI+E+Y+YM PYVS  PR AY+NYRD
Sbjct: 437 ESAIPFPHRNGTLFKILYYANWLENDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRD 496

Query: 496 LDLGRNCGR-NTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIPTSIY 545
           LD G+N      ++ EAK+WG KYFK NFDRLV++KTKVDP NFF +EQSIP   Y
Sbjct: 497 LDFGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPMPY 541

BLAST of CsaV3_1G037100 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 9.7e-151
Identity = 271/535 (50.65%), Postives = 363/535 (67.85%), Query Frame = 0

Query: 12  YLFPLFYLSFICLSSSSHSLQADSFVECFNSTSYYYSPQSIPISKVVFTN--KSAIFSSL 71
           +L  L  LS   +  S  ++Q D FV C        S  S P+    FT+   ++ F   
Sbjct: 12  FLVTLLLLSLSYIPLSFSTIQQD-FVMCLVDN----SDASFPMDSSFFTHDLNASSFKLA 71

Query: 72  LQSSIKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGHDYEGISYVS 131
           L++S +NLR+   S PKP F+  P +++HVQAA++CA K    +RLRSGGHDYEG+SYVS
Sbjct: 72  LETSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVS 131

Query: 132 SDQSQFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFPAGTCPTVGM 191
             ++ F+++DLS  R I +D+++ +A V AGA++GE+YYRI EKS  HGFPAG C ++G+
Sbjct: 132 EMETAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGI 191

Query: 192 GGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVI 251
           GGH+ GG +G++ RK+GL ADNV+DA+IVD +G+I++R +MGED+FWAIRGGGG SFGVI
Sbjct: 192 GGHIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVI 251

Query: 252 LSWKLKLVYVPSNVTVFLVQKTLEQGAIHLFQKWQTIAHKLHEDLFLHVTIGVIDEQNKT 311
           L+WK+KLV VP  VTVF V +TLEQ    L  KWQ +A KL EDLF+ V   +I   +KT
Sbjct: 252 LAWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRV---IIQPTSKT 311

Query: 312 PNMSSKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVLYFAGISIEA 371
           P    +TI  S+   FLG   RL+ +M   FP+LGL + +C E SWI+SV+Y AG    A
Sbjct: 312 PKSKERTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTA 371

Query: 372 PPEILLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILSPYGGKMRQI 431
           P E LL    +    +FKAKSD+V  PIP  GLEGLW K+LEE +   I +PYGG M +I
Sbjct: 372 PSEALLDGKSLFK-NYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKI 431

Query: 432 SDLETPFPHRKGNTFGIQYLVTWENANET-YRHLSWIREVYDYMEPYVSKYPRAAYLNYR 491
            + ETPFPHR G  F IQ+L  W++   +  +H+ W+RE+Y YME YVSK PR+AY+NYR
Sbjct: 432 PETETPFPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYR 491

Query: 492 DLDLGRNCGRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIPTSI 544
           DLDLG N G+ +   +A+ WG +YFK NF+RLV +K K DP NFF +EQSIPT +
Sbjct: 492 DLDLGMN-GKGS---DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPTEL 533

BLAST of CsaV3_1G037100 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 2.2e-150
Identity = 267/529 (50.47%), Postives = 363/529 (68.62%), Query Frame = 0

Query: 17  FYLSFICLSSSSHSLQADSFVECFNSTSYYYSPQSIPISKVVF--TNKSAIFSSLLQSSI 76
           F L  I  +SS  SLQ D F+ C    ++ Y     P+ K  F  T   ++FS +L+S+ 
Sbjct: 17  FSLYTITPTSSLASLQ-DQFINCVQRNTHVY----FPLEKTFFAPTKNVSMFSQVLESTA 76

Query: 77  KNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGHDYEGISYVSSDQSQ 136
           +NLRF   S+PKP F+ +P H+SHVQA+I+C+ K    +R+RSGGHDYEG+SYVS     
Sbjct: 77  QNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKP 136

Query: 137 FILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFPAGTCPTVGMGGHVS 196
           FIL+DLS  R ++I+++  +A V++GAT+GELYYRIAEKS  HGFPAG C ++G+GGH++
Sbjct: 137 FILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHIT 196

Query: 197 GGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKL 256
           GG +G++ RKYGL ADNV+DAKIVD NG+++DR +MGED FWAIRGG G SFG+IL+WK+
Sbjct: 197 GGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKI 256

Query: 257 KLVYVPSNVTVFLVQKTLEQGAIH-LFQKWQTIAHKLHEDLFLHVTIGVIDEQNKTPNMS 316
           KLV VP  VTVF V KTL+Q   + +  KWQ +A KL E+LF+ V        N      
Sbjct: 257 KLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF------NVAGTGG 316

Query: 317 SKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVLYFAGISIEAPPEI 376
           +KT+  S+ +LFLG    L+ +M   FPELGL   +C EMSW++S+ Y +G     P  +
Sbjct: 317 NKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNV 376

Query: 377 LLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILSPYGGKMRQISDLE 436
           LL+       + FKAKSDFV +PIP+ GL+G++ K+L+E    +I +PYGG M +I + +
Sbjct: 377 LLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWNPYGGMMAKIPESQ 436

Query: 437 TPFPHRKGNTFGIQYLVTW-ENANETYRHLSWIREVYDYMEPYVSKYPRAAYLNYRDLDL 496
            PFPHRKG  F +QY+ +W ++     RH++WIR++Y YM PYVS  PR AY+NYRDLDL
Sbjct: 437 IPFPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDL 496

Query: 497 GRNC-GRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIP 541
           GRN     T  ++A+VWG  YFKNNF+RL+ +K KVDP NFF +EQSIP
Sbjct: 497 GRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 534

BLAST of CsaV3_1G037100 vs. ExPASy TrEMBL
Match: A0A1S3BH69 (flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo OX=3656 GN=LOC103489514 PE=3 SV=1)

HSP 1 Score: 1029.2 bits (2660), Expect = 6.1e-297
Identity = 500/546 (91.58%), Postives = 524/546 (95.97%), Query Frame = 0

Query: 2   MGIPQCRIRHYLFPLFYLSFICLSSSSHSLQADSFVECFNSTSYYYSPQSIPISKVVFTN 61
           MGIPQCRIRHYLF LFYL FI LSSSS+SLQA+SF+ECFNSTSYYYSPQSIPISKVVFT+
Sbjct: 1   MGIPQCRIRHYLFLLFYLPFIWLSSSSYSLQAESFLECFNSTSYYYSPQSIPISKVVFTS 60

Query: 62  K-SAIFSSLLQSSIKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGH 121
           K SA+FSSLLQSSIKNLRF NTSL KPLFLVTPFHQSHVQ+AIVCANKKG QIR+RSGGH
Sbjct: 61  KSSALFSSLLQSSIKNLRFLNTSLQKPLFLVTPFHQSHVQSAIVCANKKGLQIRVRSGGH 120

Query: 122 DYEGISYVSSDQSQFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFP 181
           DYEGISYVSSDQSQFILLD  N+RSIDIDMKTETA VEAGATLGELYYRIAEKSP HGFP
Sbjct: 121 DYEGISYVSSDQSQFILLDFINFRSIDIDMKTETAIVEAGATLGELYYRIAEKSPIHGFP 180

Query: 182 AGTCPTVGMGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRG 241
           AG+CPTVGMGGHV GGGFGTLFRKYGLAADNVIDAKIVD NGRIMDRNSMGEDLFWAIRG
Sbjct: 181 AGSCPTVGMGGHVGGGGFGTLFRKYGLAADNVIDAKIVDVNGRIMDRNSMGEDLFWAIRG 240

Query: 242 GGGASFGVILSWKLKLVYVPSNVTVFLVQKTLEQGAIHLFQKWQTIAHKLHEDLFLHVTI 301
           GGGASFGVILSWKLKLVYVPSNVTVF+VQKTLEQGAIHLFQKWQ IAHKLHEDLFLHVT+
Sbjct: 241 GGGASFGVILSWKLKLVYVPSNVTVFVVQKTLEQGAIHLFQKWQRIAHKLHEDLFLHVTM 300

Query: 302 GVIDEQNKTPNMSSKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVL 361
           GVIDEQNKTPNMSSKTILISFVSLFLG +ERLIPLMNSHFPELGLERNNCTEMSWIQSVL
Sbjct: 301 GVIDEQNKTPNMSSKTILISFVSLFLGRIERLIPLMNSHFPELGLERNNCTEMSWIQSVL 360

Query: 362 YFAGISIEAPPEILLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILS 421
           +FAGISIE PP++LLKRPP S +LFFKAKSDFVISPIPQIG EGLWTKMLEE AS+LIL+
Sbjct: 361 FFAGISIETPPKVLLKRPPTSKMLFFKAKSDFVISPIPQIGFEGLWTKMLEEEASYLILT 420

Query: 422 PYGGKMRQISDLETPFPHRKGNTFGIQYLVTWENANETYRHLSWIREVYDYMEPYVSKYP 481
           PYGGKMRQISD +TPFPHRKGN FGIQY+VTWENANETYRHLSWIREVYDYMEPYVSK+P
Sbjct: 421 PYGGKMRQISDFDTPFPHRKGNIFGIQYMVTWENANETYRHLSWIREVYDYMEPYVSKHP 480

Query: 482 RAAYLNYRDLDLGRNCGRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIP 541
           RAAYLNYRDLDLGRNCGRNTSYEEAKVWGLKY+K+NFDRLVRVKTKVDPLNFFWNEQSIP
Sbjct: 481 RAAYLNYRDLDLGRNCGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKVDPLNFFWNEQSIP 540

Query: 542 TSIYHS 547
           TS+YHS
Sbjct: 541 TSVYHS 546

BLAST of CsaV3_1G037100 vs. ExPASy TrEMBL
Match: A0A6J1CGQ8 (berberine bridge enzyme-like 13 OS=Momordica charantia OX=3673 GN=LOC111010686 PE=3 SV=1)

HSP 1 Score: 826.2 bits (2133), Expect = 7.8e-236
Identity = 416/550 (75.64%), Postives = 469/550 (85.27%), Query Frame = 0

Query: 1   MMGIPQCRIRHYLFPLFYLSFICLSSSSHSLQADSFVECFNSTSYYYSPQS---IPISKV 60
           MMGI Q   R +LF LF++  I   SSS   Q  SF  CFNSTSYYYS  S   IPIS+V
Sbjct: 1   MMGILQ-YWRAHLFFLFFVVSIFFPSSSDYSQPQSFQXCFNSTSYYYSSHSIPIIPISEV 60

Query: 61  VFTNKSAIFSSLLQSSIKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRS 120
           +FTNKSA FSS+LQ SI+N RFFNTS PKPLFLVTPFH+ HVQAAIVCANKKG Q+R+RS
Sbjct: 61  LFTNKSASFSSVLQFSIRNFRFFNTSSPKPLFLVTPFHEFHVQAAIVCANKKGLQVRVRS 120

Query: 121 GGHDYEGISYVSSDQSQFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTH 180
           GGHDYEG+SY SS Q+ FI++DL + R+IDID+KTETA+VE GATLGELYYRIAEKSP H
Sbjct: 121 GGHDYEGLSYTSS-QAPFIVIDLIHLRAIDIDIKTETASVETGATLGELYYRIAEKSPIH 180

Query: 181 GFPAGTCPTVGMGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWA 240
           GFPAG+CPTVG+GGHVSGGGFGTLFRKYGLAADN+IDAKIVDF GRIMDRNSMGEDLFWA
Sbjct: 181 GFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNIIDAKIVDFKGRIMDRNSMGEDLFWA 240

Query: 241 IRGGGGASFGVILSWKLKLVYVPSNVTVFLVQKTLEQGAIHLFQKWQTIAHKLHEDLFLH 300
           IRGGG ASFGVILSWKLKLV VPS VTVFLVQKTLEQGA  LFQKWQTIA KL E LFLH
Sbjct: 241 IRGGGXASFGVILSWKLKLVSVPSKVTVFLVQKTLEQGATQLFQKWQTIADKLDEHLFLH 300

Query: 301 VTIGVIDEQNKTP-NMSSKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWI 360
           V IGV + QNK+P   S+KTIL+SF SLFLGP+++L  +M++HFPELGL+R N TEMSWI
Sbjct: 301 VIIGVTENQNKSPKEPSTKTILLSFESLFLGPIDKLSLIMSTHFPELGLQRENYTEMSWI 360

Query: 361 QSVLYFAGISIEAPPEILLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASF 420
           QSVL+F G+S EAP EILL+RPP+S++ FFKAKSD+V SPIPQ GLE LW+K+LEE AS 
Sbjct: 361 QSVLFFTGLSTEAPLEILLERPPLSDI-FFKAKSDYVTSPIPQTGLEALWSKVLEEEASL 420

Query: 421 LILSPYGGKMRQISDLETPFPHRKGNTFGIQYLVTWENANETYRHLSWIREVYDYMEPYV 480
           LIL+PYGGKM QIS+ ETPFPHR+G  FGIQY+VTWEN NET +HLSW+REVY  ME YV
Sbjct: 421 LILTPYGGKMSQISESETPFPHRRGIIFGIQYMVTWENGNETEKHLSWMREVYTCMELYV 480

Query: 481 SKYPRAAYLNYRDLDLGRNCGRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNE 540
           SK PR+AYL+YRDLDLGRN GRN SYEEAKVWGLKYFK NF+RLVRVKTKVDP N FWNE
Sbjct: 481 SKCPRSAYLSYRDLDLGRNYGRNRSYEEAKVWGLKYFKGNFERLVRVKTKVDPSNLFWNE 540

Query: 541 QSIPTSIYHS 547
           QSIP +++HS
Sbjct: 541 QSIPPTLHHS 547

BLAST of CsaV3_1G037100 vs. ExPASy TrEMBL
Match: A0A6J1KH65 (berberine bridge enzyme-like 15 OS=Cucurbita maxima OX=3661 GN=LOC111495709 PE=3 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 2.2e-230
Identity = 405/533 (75.98%), Postives = 460/533 (86.30%), Query Frame = 0

Query: 16  LFYLSFICLSSS--SHSLQADSFVECFNSTSYYYSPQSIPISKVVFTNKSAIFSSLLQSS 75
           LFYL  I LSSS  SH+LQ  SFVECF+S   Y SPQSIPISKVVFTN+SA FSSLL  S
Sbjct: 5   LFYLPLIWLSSSSPSHALQ-QSFVECFSS---YSSPQSIPISKVVFTNESASFSSLLHQS 64

Query: 76  IKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGHDYEGISYVSSDQS 135
           I+NLRF NTS  KPLFLVTPFH+SHVQAA+VCA KKG Q+R+RSGGHDYEG+SY+SS QS
Sbjct: 65  IRNLRFLNTS-SKPLFLVTPFHESHVQAAVVCAKKKGLQLRVRSGGHDYEGLSYLSS-QS 124

Query: 136 QFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFPAGTCPTVGMGGHV 195
           QFI+LDL+N RSID+D++TETA+VE GATLGE YYRIAEKS  HGFPAG+CPTVG+GGH+
Sbjct: 125 QFIVLDLTNLRSIDVDIETETASVETGATLGEFYYRIAEKSLIHGFPAGSCPTVGVGGHI 184

Query: 196 SGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWK 255
           SGGGFGTLFRK+GLAAD VIDAKIVDFNGRIMDRN+MGE+LFWAIRGGGGASFGVILSWK
Sbjct: 185 SGGGFGTLFRKHGLAADKVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWK 244

Query: 256 LKLVYVPSNVTVFLVQKTLEQGAIHLFQKWQTIAHKLHEDLFLHVTIGVIDEQNKTPNMS 315
           LKLV +P  +T+F VQKT +QGA+ LFQKWQ IA+KL EDLFLHVTIGV           
Sbjct: 245 LKLVSLPPKLTIFHVQKTPKQGAMQLFQKWQRIAYKLDEDLFLHVTIGV----------Q 304

Query: 316 SKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVLYFAGISIEAPPEI 375
            +TI +SFV+LFLGP+E+LIPLMN+ FPELGL+R N TEM+WIQS+L+FAG  I+ PP+I
Sbjct: 305 EQTISLSFVALFLGPIEKLIPLMNTQFPELGLQRENYTEMNWIQSILFFAGFPIQTPPQI 364

Query: 376 LLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILSPYGGKMRQISDLE 435
           LLK+ P+SN  FFKAKSDFV  PIPQ GLEGLW K+LEE  S+LIL+PYGGKM QIS+LE
Sbjct: 365 LLKK-PLSNA-FFKAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELE 424

Query: 436 TPFPHRKGNTFGIQYLVTWENANETYRHLSWIREVYDYMEPYVSKYPRAAYLNYRDLDLG 495
           TPFPHRKGN FGIQY+V+WEN +E+Y+HLSWIREVY YMEPYVSK PR AYLNYRDLDLG
Sbjct: 425 TPFPHRKGNIFGIQYMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLG 484

Query: 496 RNCGRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIPTSIYHS 547
           RN GRN  YEEAKVWGLKY+K+NF+RLVRVKTKVDP NFFWNEQSIPTS+Y S
Sbjct: 485 RNYGRNVRYEEAKVWGLKYYKSNFERLVRVKTKVDPSNFFWNEQSIPTSLYRS 519

BLAST of CsaV3_1G037100 vs. ExPASy TrEMBL
Match: A0A6J1EGS9 (berberine bridge enzyme-like 15 OS=Cucurbita moschata OX=3662 GN=LOC111434055 PE=3 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 8.3e-230
Identity = 407/533 (76.36%), Postives = 459/533 (86.12%), Query Frame = 0

Query: 16  LFYLSFICLSSS--SHSLQADSFVECFNSTSYYYSPQSIPISKVVFTNKSAIFSSLLQSS 75
           LFYL  I LSSS  SH+LQ  SFVECF+S   Y SPQSIPISKVVFTN+SA FSSLLQ S
Sbjct: 5   LFYLPLIWLSSSSPSHALQ-QSFVECFSS---YSSPQSIPISKVVFTNESASFSSLLQQS 64

Query: 76  IKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGHDYEGISYVSSDQS 135
           I+NLRF NTS  KPLFLVTPFH+SHVQAA+VCA KKG Q+R+RSGGHDYEG+SYVSS QS
Sbjct: 65  IRNLRFLNTS-SKPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSS-QS 124

Query: 136 QFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFPAGTCPTVGMGGHV 195
           QFILLDLSN  SIDID++TETA+VE GATLGELYYRIAEKS  HGFPAG+CPTVG+GGH+
Sbjct: 125 QFILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHI 184

Query: 196 SGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWK 255
           SGGGFGTLFRK+GLAADNVIDAKIVDFNGRIMDRN+MGE+LFWAIRGGGGASFGVILSWK
Sbjct: 185 SGGGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWK 244

Query: 256 LKLVYVPSNVTVFLVQKTLEQGAIHLFQKWQTIAHKLHEDLFLHVTIGVIDEQNKTPNMS 315
           LKLV +P  +T+F VQKT +QGAI LFQKWQ IA+KL EDLFLHVTIGV           
Sbjct: 245 LKLVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGV----------Q 304

Query: 316 SKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVLYFAGISIEAPPEI 375
            +TI +SFV+LFLGP+E+LIPLMN+ FPEL L+R N TEM+WIQS+L+FAG  I+ PP+I
Sbjct: 305 EQTISLSFVALFLGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQI 364

Query: 376 LLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILSPYGGKMRQISDLE 435
           LLK+  +SN  FFKAKSDFV  PIPQ GLEGLW K+LEE  S+LIL+PYGGKM QIS+LE
Sbjct: 365 LLKK-SLSNA-FFKAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELE 424

Query: 436 TPFPHRKGNTFGIQYLVTWENANETYRHLSWIREVYDYMEPYVSKYPRAAYLNYRDLDLG 495
           TPFPHRKGN FGIQY+V+WEN +E+Y+HLSWIREVY YMEPYVSK PR AYLNYRDLDLG
Sbjct: 425 TPFPHRKGNIFGIQYMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLG 484

Query: 496 RNCGRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIPTSIYHS 547
           RN GRN  YEEA+VWGLKY+++NF+RLVRVKTKVDP NFFWNEQSIP S+Y S
Sbjct: 485 RNYGRNVRYEEAEVWGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSLYRS 519

BLAST of CsaV3_1G037100 vs. ExPASy TrEMBL
Match: A0A6J1CHF0 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111010911 PE=3 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 1.3e-227
Identity = 399/534 (74.72%), Postives = 454/534 (85.02%), Query Frame = 0

Query: 16  LFYLSFICLSSSSHSLQADSFVECFNSTSYYYSPQSIPISKVVFTNKSAIFSSLLQSSIK 75
           LF + F C+SSS   LQ +SF+ECFNST+  YS  S+PIS+VVFTN+SA FSSLL+ SI+
Sbjct: 11  LFLIFFHCVSSSD-CLQ-ESFMECFNSTT--YSKHSVPISEVVFTNESASFSSLLRLSIR 70

Query: 76  NLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGHDYEGISYVSSDQSQF 135
           NLRF   S PKP FLVTPF  SHVQAA+VCA KK  Q+R+RSGGHDYEG+SYVSS Q+ F
Sbjct: 71  NLRFLTNSSPKPQFLVTPFRASHVQAAVVCAKKKSLQVRVRSGGHDYEGLSYVSS-QTPF 130

Query: 136 ILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFPAGTCPTVGMGGHVSG 195
           I++DL N  SI ID++TETA+VE GATLGE+YYRIAEKSP HGFPAG+CPTVG+GGHVSG
Sbjct: 131 IVIDLINLSSIHIDIETETASVETGATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGHVSG 190

Query: 196 GGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLK 255
           GGFGTLFRKYGLAADNVIDAKIVDFNGRI DRNSMGEDLFWAIRGGGGASFGVILSWKLK
Sbjct: 191 GGFGTLFRKYGLAADNVIDAKIVDFNGRIRDRNSMGEDLFWAIRGGGGASFGVILSWKLK 250

Query: 256 LVYVPSNVTVFLVQKTLEQGAIHLFQKWQTIAHKLHEDLFLHVTIGVIDEQNK---TPNM 315
           LV VP +VTVF V +TL+QGA HLFQKWQ IAHK+ +DLFLHVT  V+D + K   +   
Sbjct: 251 LVSVPPDVTVFNVHRTLDQGATHLFQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSSKEA 310

Query: 316 SSKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVLYFAGISIEAPPE 375
           SSKTI +SF SLFLGP+ERLIPLMN+HF ELGL+RN+CTEMSWIQSVL+FA +S E PPE
Sbjct: 311 SSKTIKLSFTSLFLGPIERLIPLMNTHFHELGLKRNDCTEMSWIQSVLFFADLSTETPPE 370

Query: 376 ILLKR-PPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILSPYGGKMRQISD 435
           IL++R PPIS+  FF AKSD+V SPI Q GLE LW+K+LEE  S LIL+PYGGKM QIS+
Sbjct: 371 ILMERSPPISDT-FFTAKSDYVTSPISQTGLEALWSKLLEEEKSELILTPYGGKMDQISE 430

Query: 436 LETPFPHRKGNTFGIQYLVTWENANETYRHLSWIREVYDYMEPYVSKYPRAAYLNYRDLD 495
            ETPFPHR+GN +GIQYL  WENANET +HLSW+REVY YM+P+VSKYPR AYLNYRDLD
Sbjct: 431 SETPFPHRRGNIYGIQYLAAWENANETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRDLD 490

Query: 496 LGRNCGRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIPTSIYH 546
           LGRN  RNTSY+EAKVWGLKYFK NF+RLVRVKTKVDPLNFFWNEQSIP   +H
Sbjct: 491 LGRNYERNTSYDEAKVWGLKYFKGNFERLVRVKTKVDPLNFFWNEQSIPLCHHH 538

BLAST of CsaV3_1G037100 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 551.2 bits (1419), Expect = 9.3e-157
Identity = 278/531 (52.35%), Postives = 366/531 (68.93%), Query Frame = 0

Query: 16  LFYLSFICLSSSSHSLQADSFVECFNSTSYYYSPQSIPISKVVFT--NKSAIFSSLLQSS 75
           L  L  I +  SS +LQ D FV+C        S  S PI+   F+    + +F   L+S+
Sbjct: 14  LVTLLLISVPLSSSTLQQD-FVKCLVDN----SDVSFPITASFFSPDQNATLFKEELEST 73

Query: 76  IKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGHDYEGISYVSSDQS 135
            +NLR+   S PKP+F+  P +++HVQAA+VCA K    +RLRSGGHDYEG+S+V+ D++
Sbjct: 74  AQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDET 133

Query: 136 QFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFPAGTCPTVGMGGHV 195
            F+++DLS  R +D+D+ + +A   AGAT+GE+YYRI EKS THGFPAG C ++G+GGH+
Sbjct: 134 PFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHL 193

Query: 196 SGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWK 255
            GG +G++ RK+GL ADNV+DA+IVD NG+I+DR +MGED+FWAIRGGGG SFGVIL+WK
Sbjct: 194 VGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWK 253

Query: 256 LKLVYVPSNVTVFLVQKTLEQGAIHLFQKWQTIAHKLHEDLFLHVTIGVIDEQNKTPNMS 315
           +KLV VP+ VTVF V KTLEQ    +  KW+ IA KL +DLF+ V   +I   +KT    
Sbjct: 254 IKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRV---IISPASKTTKPG 313

Query: 316 SKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVLYFAGISIEAPPEI 375
           ++TI +S+ + FLG   RL+ +M   FPELGL + +CTEMSWI+SV+Y AG    A PE 
Sbjct: 314 NRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEA 373

Query: 376 LLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILSPYGGKMRQISDLE 435
           LL    +     FKAKSDFV  PIP  GLEGLW + LEE +   I +PYGG M +IS+ E
Sbjct: 374 LLAGKSLFK-NHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESE 433

Query: 436 TPFPHRKGNTFGIQYLVTWENAN-ETYRHLSWIREVYDYMEPYVSKYPRAAYLNYRDLDL 495
            PFPHR G  F IQ+L TW++      RH+ WIRE+Y YME YVSK PR AY+NYRDLDL
Sbjct: 434 IPFPHRNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDL 493

Query: 496 GRNCGRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIPTSI 544
           G N G      +A+ WG KY+K NF+RLV++K + DP NFF +EQS+PT I
Sbjct: 494 GTNEGET----DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPTKI 531

BLAST of CsaV3_1G037100 vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 546.2 bits (1406), Expect = 3.0e-155
Identity = 279/537 (51.96%), Postives = 374/537 (69.65%), Query Frame = 0

Query: 13  LFPLFYLSFICLSSSSHSLQADSFVECFNSTSYYYSPQSIPISKVVFT--NKSAIFSSLL 72
           +F L++  +    +SS SLQ D F++C     Y  +     + K  FT    ++IF+ +L
Sbjct: 15  VFALYFSFYTITLTSSTSLQ-DDFIKCL----YRNTNVRFTLDKTFFTPERNASIFTEVL 74

Query: 73  QSSIKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGHDYEGISYVSS 132
           +S+ +N R+   ++PKP F+  P H+SHVQA+++C+ K     R+RSGGHDYEG+SYVS 
Sbjct: 75  ESTAQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQ 134

Query: 133 DQSQFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFPAGTCPTVGMG 192
            +  F+L+DLS  R I++D+K  +A VEAGAT+GELYYRIAEKS  HGFPAG  P++G+G
Sbjct: 135 IEKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIG 194

Query: 193 GHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVIL 252
           GH++GG +G+L RKYGLAADNV+DAKIVD NG+++DR SMGEDLFWAIRGG G SFG+IL
Sbjct: 195 GHITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIIL 254

Query: 253 SWKLKLVYVPSNVTVFLVQKTLEQG-AIHLFQKWQTIAHKLHEDLFLHVTIGVIDEQNKT 312
           SWK+KLV VP  +TVF V KT EQ  +  +  KWQ IA  L ++LFL V   V      +
Sbjct: 255 SWKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTV------S 314

Query: 313 PNMSSKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVLYFAGISIEA 372
            N ++KT+ ++++  FLG    L+ +M   FPELGL + +C EMSWI S++Y +G     
Sbjct: 315 GNKANKTVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNP 374

Query: 373 PP--EILLK-RPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILSPYGGKM 432
           PP  EILL+ + PI  V +FK KSDF   PIP +GLEG++ K+LEE A+ +I +PYGGKM
Sbjct: 375 PPPIEILLQAKSPIGKV-YFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGGKM 434

Query: 433 RQISDLETPFPHRKGNTFGIQYLVTWENANE-TYRHLSWIREVYDYMEPYVSKYPRAAYL 492
            +I + E PFPHR G  F IQY  +W ++ +   R   WIRE+Y YM PYVS  PR AY+
Sbjct: 435 DKIPESEIPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAYV 494

Query: 493 NYRDLDLGRNCGRNTS-YEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIPT 542
           NYRDLDLG+N   + S + EAK+WG  YFK+NF+RLVR+K+KVDP NFF +EQSIPT
Sbjct: 495 NYRDLDLGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPT 539

BLAST of CsaV3_1G037100 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 539.3 bits (1388), Expect = 3.7e-153
Identity = 271/536 (50.56%), Postives = 368/536 (68.66%), Query Frame = 0

Query: 16  LFYLSFICL--SSSSHSLQADSFVECFNSTSYYYSPQSIPISKVVFT--NKSAIFSSLLQ 75
           + Y SF C+  +SSS S+Q D F+ C    ++     S P+ K +FT     ++F+ +L+
Sbjct: 17  VLYFSFYCITPTSSSASIQ-DQFINCVKRNTHV----SFPLEKTLFTPAKNVSLFNQVLE 76

Query: 76  SSIKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGHDYEGISYVSSD 135
           S+ +NL+F   S+PKP F+  P HQS VQA+I+C+ K G   R+RSGGHD+E +SYVS  
Sbjct: 77  STAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRI 136

Query: 136 QSQFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFPAGTCPTVGMGG 195
           +  FILLDLS  + I++D+++ +A V+ GATLGELYYRIAEKS  HGFPAG C +VG+GG
Sbjct: 137 EKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGG 196

Query: 196 HVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILS 255
           +++GGG+GTL RKYGLA DNV+D K+VD NG+++DR +MGEDLFWAIRGGGGASFG++L+
Sbjct: 197 YMTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLA 256

Query: 256 WKLKLVYVPSNVTVFLVQKTLEQGA-IHLFQKWQTIAHKLHEDLFLHVTIGVIDEQNKTP 315
           WK+KLV VP  VTVF V KTLEQ A +    KWQ I+ K+ E++ + V +          
Sbjct: 257 WKIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVL------RAAG 316

Query: 316 NMSSKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVLYFAGISIEAP 375
           N  +KT+ ++++  FLG    L+ +M   FPELGL + +CTEMSWI++ L+  G    +P
Sbjct: 317 NDGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSP 376

Query: 376 PEILLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILSPYGGKMRQIS 435
            EILL+        +FKA SDFV  PIP IGL+G++ +++E   +FL  +PYGG M +I 
Sbjct: 377 IEILLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTFLNWTPYGGMMSKIP 436

Query: 436 DLETPFPHRKGNTFGIQYLVTW-ENANETYRHLSWIREVYDYMEPYVSKYPRAAYLNYRD 495
           +   PFPHR G  F I Y   W EN   + R ++WI+E+Y+YM PYVS  PR AY+NYRD
Sbjct: 437 ESAIPFPHRNGTLFKILYYANWLENDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRD 496

Query: 496 LDLGRNCGR-NTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIPTSIY 545
           LD G+N      ++ EAK+WG KYFK NFDRLV++KTKVDP NFF +EQSIP   Y
Sbjct: 497 LDFGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPMPY 541

BLAST of CsaV3_1G037100 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 535.0 bits (1377), Expect = 6.9e-152
Identity = 271/535 (50.65%), Postives = 363/535 (67.85%), Query Frame = 0

Query: 12  YLFPLFYLSFICLSSSSHSLQADSFVECFNSTSYYYSPQSIPISKVVFTN--KSAIFSSL 71
           +L  L  LS   +  S  ++Q D FV C        S  S P+    FT+   ++ F   
Sbjct: 12  FLVTLLLLSLSYIPLSFSTIQQD-FVMCLVDN----SDASFPMDSSFFTHDLNASSFKLA 71

Query: 72  LQSSIKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGHDYEGISYVS 131
           L++S +NLR+   S PKP F+  P +++HVQAA++CA K    +RLRSGGHDYEG+SYVS
Sbjct: 72  LETSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVS 131

Query: 132 SDQSQFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFPAGTCPTVGM 191
             ++ F+++DLS  R I +D+++ +A V AGA++GE+YYRI EKS  HGFPAG C ++G+
Sbjct: 132 EMETAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGI 191

Query: 192 GGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVI 251
           GGH+ GG +G++ RK+GL ADNV+DA+IVD +G+I++R +MGED+FWAIRGGGG SFGVI
Sbjct: 192 GGHIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVI 251

Query: 252 LSWKLKLVYVPSNVTVFLVQKTLEQGAIHLFQKWQTIAHKLHEDLFLHVTIGVIDEQNKT 311
           L+WK+KLV VP  VTVF V +TLEQ    L  KWQ +A KL EDLF+ V   +I   +KT
Sbjct: 252 LAWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRV---IIQPTSKT 311

Query: 312 PNMSSKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVLYFAGISIEA 371
           P    +TI  S+   FLG   RL+ +M   FP+LGL + +C E SWI+SV+Y AG    A
Sbjct: 312 PKSKERTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTA 371

Query: 372 PPEILLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILSPYGGKMRQI 431
           P E LL    +    +FKAKSD+V  PIP  GLEGLW K+LEE +   I +PYGG M +I
Sbjct: 372 PSEALLDGKSLFK-NYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKI 431

Query: 432 SDLETPFPHRKGNTFGIQYLVTWENANET-YRHLSWIREVYDYMEPYVSKYPRAAYLNYR 491
            + ETPFPHR G  F IQ+L  W++   +  +H+ W+RE+Y YME YVSK PR+AY+NYR
Sbjct: 432 PETETPFPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYR 491

Query: 492 DLDLGRNCGRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIPTSI 544
           DLDLG N G+ +   +A+ WG +YFK NF+RLV +K K DP NFF +EQSIPT +
Sbjct: 492 DLDLGMN-GKGS---DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPTEL 533

BLAST of CsaV3_1G037100 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 533.9 bits (1374), Expect = 1.5e-151
Identity = 267/529 (50.47%), Postives = 363/529 (68.62%), Query Frame = 0

Query: 17  FYLSFICLSSSSHSLQADSFVECFNSTSYYYSPQSIPISKVVF--TNKSAIFSSLLQSSI 76
           F L  I  +SS  SLQ D F+ C    ++ Y     P+ K  F  T   ++FS +L+S+ 
Sbjct: 17  FSLYTITPTSSLASLQ-DQFINCVQRNTHVY----FPLEKTFFAPTKNVSMFSQVLESTA 76

Query: 77  KNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGHDYEGISYVSSDQSQ 136
           +NLRF   S+PKP F+ +P H+SHVQA+I+C+ K    +R+RSGGHDYEG+SYVS     
Sbjct: 77  QNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKP 136

Query: 137 FILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFPAGTCPTVGMGGHVS 196
           FIL+DLS  R ++I+++  +A V++GAT+GELYYRIAEKS  HGFPAG C ++G+GGH++
Sbjct: 137 FILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHIT 196

Query: 197 GGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKL 256
           GG +G++ RKYGL ADNV+DAKIVD NG+++DR +MGED FWAIRGG G SFG+IL+WK+
Sbjct: 197 GGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKI 256

Query: 257 KLVYVPSNVTVFLVQKTLEQGAIH-LFQKWQTIAHKLHEDLFLHVTIGVIDEQNKTPNMS 316
           KLV VP  VTVF V KTL+Q   + +  KWQ +A KL E+LF+ V        N      
Sbjct: 257 KLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF------NVAGTGG 316

Query: 317 SKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVLYFAGISIEAPPEI 376
           +KT+  S+ +LFLG    L+ +M   FPELGL   +C EMSW++S+ Y +G     P  +
Sbjct: 317 NKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNV 376

Query: 377 LLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILSPYGGKMRQISDLE 436
           LL+       + FKAKSDFV +PIP+ GL+G++ K+L+E    +I +PYGG M +I + +
Sbjct: 377 LLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWNPYGGMMAKIPESQ 436

Query: 437 TPFPHRKGNTFGIQYLVTW-ENANETYRHLSWIREVYDYMEPYVSKYPRAAYLNYRDLDL 496
            PFPHRKG  F +QY+ +W ++     RH++WIR++Y YM PYVS  PR AY+NYRDLDL
Sbjct: 437 IPFPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDL 496

Query: 497 GRNC-GRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIP 541
           GRN     T  ++A+VWG  YFKNNF+RL+ +K KVDP NFF +EQSIP
Sbjct: 497 GRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 534