CsaV3_1G024440 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_1G024440
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Descriptionprotein NRT1/ PTR FAMILY 1.2-like
Locationchr1: 14040878 .. 14042885 (+)
RNA-Seq ExpressionCsaV3_1G024440
SyntenyCsaV3_1G024440
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAACCAGAATTCAGAGAAAATGGAAACCACCCAAAAGGTTGAAAGAAAAAAGGGTGGTCTTCGAACTATGCCTTTTATTATAGGTAATTTTCAACAAGTTCTAAAAGTTCTGAAGCATAAATTAGTAATATGATCACTAACAAAAACTCCAAATCTGCAGCAAACGAGATATTTGAGAAGATTTCAAATGTTGGTCTTCATGCAAATATGATATTTTACTTGATGAATGAGTATCATCTGGATAGTGCTAAAGGAGCTATCGTCTTGTTTCTGTGGTCTGCATTGACAAACTTATTGCCCATTGGTGGGGCTTTCCTCTCTGATTCTTACTTGGGTCGATTTAAGGTCATCGCCATGGGGACAGTGGTTACACTTGTTGTAAGCAAGGATTCATTTGCTTAGTTCCAACCTGAATATCTGTTTGTTTCATCTGTTTTTCAACTTTGATCTTTCTTCGGACTTCAGGGAATGGTGGTTTTATGGTTAACAGCCATATTTCCAAAAGCAAGGCCTCCACACTGTACACCAGCTGGGGAATTTTGTGTTTCAGCAAACACTGCTCAGCTGATGCTTCTGTATTTTGCATTTCTTCTTATGGCAGCCGGAGCTGGGGGAATTCGACCATGTTCCTTGGCGTTTGGAGCAGATCAACTTGAAAATCCAGGCAACCCCAGAAACCAGAGGACTATGCAGAGCTTCTTCAACTGGTACTATGCTTCAGTTGGAATTTCAGTCACCATTTCGGTGGTCTTTATGGTTTATCTCCAGAATGCTACCGGTTGGGTTGTGGGATTTGGAGTTCCTGTTGGACTTATGCTGTTTTCCACAGCCATGTTCTTTCTTGGCTCTTCATTGTATGTTAAACTGATGGCGAACAAGAGTCTGCTTAGCAGTTTGACTCAGGTAATCGTTGCAGCTTGGAGAAATAGACACTTGGAATTTCCCCCACAAGCTTCTGATAAATGGTTTTACCATAAAGGTTCAAAGCTTGTCACCCCAACGCCTAAACTGAGGTATGTTAATAATCAAAACGTTCCATAATTATTGCTTGTTATGCTTTTAGATTTTCTAAAATGATCCAGGTTGATTCTTCTGTCTTGCTTTCAAGGTTTCTAAACAAGGCTTGCATAATAAGAAACATAGAAACAGATCTAGATTCTAATGGAATGGCCAAATTCCCATGGAGATTAAGCACGATTCAACGTGTAGAAGAACTAAAAGCAGTAATCAGAGTACTTCCGATTTGGTCCACTGGAATTGTAATCAGTGCCACCATTAACCAATTCACCTTCTATTCTCTCCAAGCTAGCACAATGGATAGACATATAACACCTCACTTTCAATTCCCAGCGGCTTCCTTCGCTGTTTTCACAATCCTAACCCTTACAATCTGGGTCGCAATATACGATCAAATAATCGTTCCACTACTAGCGAAGTTCACCAAAAAATCGAATGGACTCACTTTAAAGCAGAGAATGGGCATCGGACTGGTAATCTCATGCTTGGCCTCAATAGTGTCGGCAGAAATAGAGAGAAAACGAAGAAACAGAGCAATTCATGAAGGTCTAGCCAACGTACCAGGAGGAATTGTGCAGTTGTCAGCGATGTGGCTGGTTCCACAACACTGCCTGGCGGGGTTGGCTGAAGCGTTGAACGCAATAGGACAGATTCAATTCTTCTATTCGCAGCTGCCGAGGAGCATGGCCAGCATTGCAGTAGCGCTGTTCTCGCTAGGAATGGGCGGAGGGAGCTTGGTGGCAGCGCTGACTGTAAGCGTGGTGAAAAAGAGGACAAAGAAGAATGGGAAGGGATGGCTGTCAAATAATCTGAACCAGGGGCATTATGATTACTACTACTGGGTGTTGAGCTTGATGGGCGTCGCTAATTTGTTGTACTATTTGATCTGCAGTTGGTTCTATGGAGATGAGAAAGAGGGAATGGAACAGAGTAGGATTTGGGATGATAAGGAAGCCATTGAAGCGGAGGCCACCTTCAATTCTTGA

mRNA sequence

ATGGAGAACCAGAATTCAGAGAAAATGGAAACCACCCAAAAGGTTGAAAGAAAAAAGGGTGGTCTTCGAACTATGCCTTTTATTATAGCAAACGAGATATTTGAGAAGATTTCAAATGTTGGTCTTCATGCAAATATGATATTTTACTTGATGAATGAGTATCATCTGGATAGTGCTAAAGGAGCTATCGTCTTGTTTCTGTGGTCTGCATTGACAAACTTATTGCCCATTGGTGGGGCTTTCCTCTCTGATTCTTACTTGGGTCGATTTAAGGTCATCGCCATGGGGACAGTGGTTACACTTGTTGGAATGGTGGTTTTATGGTTAACAGCCATATTTCCAAAAGCAAGGCCTCCACACTGTACACCAGCTGGGGAATTTTGTGTTTCAGCAAACACTGCTCAGCTGATGCTTCTGTATTTTGCATTTCTTCTTATGGCAGCCGGAGCTGGGGGAATTCGACCATGTTCCTTGGCGTTTGGAGCAGATCAACTTGAAAATCCAGGCAACCCCAGAAACCAGAGGACTATGCAGAGCTTCTTCAACTGGTACTATGCTTCAGTTGGAATTTCAGTCACCATTTCGGTGGTCTTTATGGTTTATCTCCAGAATGCTACCGGTTGGGTTGTGGGATTTGGAGTTCCTGTTGGACTTATGCTGTTTTCCACAGCCATGTTCTTTCTTGGCTCTTCATTGTATGTTAAACTGATGGCGAACAAGAGTCTGCTTAGCAGTTTGACTCAGGTAATCGTTGCAGCTTGGAGAAATAGACACTTGGAATTTCCCCCACAAGCTTCTGATAAATGGTTTTACCATAAAGGTTCAAAGCTTGTCACCCCAACGCCTAAACTGAGGTTTCTAAACAAGGCTTGCATAATAAGAAACATAGAAACAGATCTAGATTCTAATGGAATGGCCAAATTCCCATGGAGATTAAGCACGATTCAACGTGTAGAAGAACTAAAAGCAGTAATCAGAGTACTTCCGATTTGGTCCACTGGAATTGTAATCAGTGCCACCATTAACCAATTCACCTTCTATTCTCTCCAAGCTAGCACAATGGATAGACATATAACACCTCACTTTCAATTCCCAGCGGCTTCCTTCGCTGTTTTCACAATCCTAACCCTTACAATCTGGGTCGCAATATACGATCAAATAATCGTTCCACTACTAGCGAAGTTCACCAAAAAATCGAATGGACTCACTTTAAAGCAGAGAATGGGCATCGGACTGGTAATCTCATGCTTGGCCTCAATAGTGTCGGCAGAAATAGAGAGAAAACGAAGAAACAGAGCAATTCATGAAGGTCTAGCCAACGTACCAGGAGGAATTGTGCAGTTGTCAGCGATGTGGCTGGTTCCACAACACTGCCTGGCGGGGTTGGCTGAAGCGTTGAACGCAATAGGACAGATTCAATTCTTCTATTCGCAGCTGCCGAGGAGCATGGCCAGCATTGCAGTAGCGCTGTTCTCGCTAGGAATGGGCGGAGGGAGCTTGGTGGCAGCGCTGACTGTAAGCGTGGTGAAAAAGAGGACAAAGAAGAATGGGAAGGGATGGCTGTCAAATAATCTGAACCAGGGGCATTATGATTACTACTACTGGGTGTTGAGCTTGATGGGCGTCGCTAATTTGTTGTACTATTTGATCTGCAGTTGGTTCTATGGAGATGAGAAAGAGGGAATGGAACAGAGTAGGATTTGGGATGATAAGGAAGCCATTGAAGCGGAGGCCACCTTCAATTCTTGA

Coding sequence (CDS)

ATGGAGAACCAGAATTCAGAGAAAATGGAAACCACCCAAAAGGTTGAAAGAAAAAAGGGTGGTCTTCGAACTATGCCTTTTATTATAGCAAACGAGATATTTGAGAAGATTTCAAATGTTGGTCTTCATGCAAATATGATATTTTACTTGATGAATGAGTATCATCTGGATAGTGCTAAAGGAGCTATCGTCTTGTTTCTGTGGTCTGCATTGACAAACTTATTGCCCATTGGTGGGGCTTTCCTCTCTGATTCTTACTTGGGTCGATTTAAGGTCATCGCCATGGGGACAGTGGTTACACTTGTTGGAATGGTGGTTTTATGGTTAACAGCCATATTTCCAAAAGCAAGGCCTCCACACTGTACACCAGCTGGGGAATTTTGTGTTTCAGCAAACACTGCTCAGCTGATGCTTCTGTATTTTGCATTTCTTCTTATGGCAGCCGGAGCTGGGGGAATTCGACCATGTTCCTTGGCGTTTGGAGCAGATCAACTTGAAAATCCAGGCAACCCCAGAAACCAGAGGACTATGCAGAGCTTCTTCAACTGGTACTATGCTTCAGTTGGAATTTCAGTCACCATTTCGGTGGTCTTTATGGTTTATCTCCAGAATGCTACCGGTTGGGTTGTGGGATTTGGAGTTCCTGTTGGACTTATGCTGTTTTCCACAGCCATGTTCTTTCTTGGCTCTTCATTGTATGTTAAACTGATGGCGAACAAGAGTCTGCTTAGCAGTTTGACTCAGGTAATCGTTGCAGCTTGGAGAAATAGACACTTGGAATTTCCCCCACAAGCTTCTGATAAATGGTTTTACCATAAAGGTTCAAAGCTTGTCACCCCAACGCCTAAACTGAGGTTTCTAAACAAGGCTTGCATAATAAGAAACATAGAAACAGATCTAGATTCTAATGGAATGGCCAAATTCCCATGGAGATTAAGCACGATTCAACGTGTAGAAGAACTAAAAGCAGTAATCAGAGTACTTCCGATTTGGTCCACTGGAATTGTAATCAGTGCCACCATTAACCAATTCACCTTCTATTCTCTCCAAGCTAGCACAATGGATAGACATATAACACCTCACTTTCAATTCCCAGCGGCTTCCTTCGCTGTTTTCACAATCCTAACCCTTACAATCTGGGTCGCAATATACGATCAAATAATCGTTCCACTACTAGCGAAGTTCACCAAAAAATCGAATGGACTCACTTTAAAGCAGAGAATGGGCATCGGACTGGTAATCTCATGCTTGGCCTCAATAGTGTCGGCAGAAATAGAGAGAAAACGAAGAAACAGAGCAATTCATGAAGGTCTAGCCAACGTACCAGGAGGAATTGTGCAGTTGTCAGCGATGTGGCTGGTTCCACAACACTGCCTGGCGGGGTTGGCTGAAGCGTTGAACGCAATAGGACAGATTCAATTCTTCTATTCGCAGCTGCCGAGGAGCATGGCCAGCATTGCAGTAGCGCTGTTCTCGCTAGGAATGGGCGGAGGGAGCTTGGTGGCAGCGCTGACTGTAAGCGTGGTGAAAAAGAGGACAAAGAAGAATGGGAAGGGATGGCTGTCAAATAATCTGAACCAGGGGCATTATGATTACTACTACTGGGTGTTGAGCTTGATGGGCGTCGCTAATTTGTTGTACTATTTGATCTGCAGTTGGTTCTATGGAGATGAGAAAGAGGGAATGGAACAGAGTAGGATTTGGGATGATAAGGAAGCCATTGAAGCGGAGGCCACCTTCAATTCTTGA

Protein sequence

MENQNSEKMETTQKVERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSAKGAIVLFLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPPHCTPAGEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQSFFNWYYASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMANKSLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNIETDLDSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQFTFYSLQASTMDRHITPHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVISCLASIVSAEIERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLPRSMASIAVALFSLGMGGGSLVAALTVSVVKKRTKKNGKGWLSNNLNQGHYDYYYWVLSLMGVANLLYYLICSWFYGDEKEGMEQSRIWDDKEAIEAEATFNS*
Homology
BLAST of CsaV3_1G024440 vs. NCBI nr
Match: KAE8652969.1 (hypothetical protein Csa_017739 [Cucumis sativus])

HSP 1 Score: 1146.3 bits (2964), Expect = 0.0e+00
Identity = 582/582 (100.00%), Postives = 582/582 (100.00%), Query Frame = 0

Query: 1   MENQNSEKMETTQKVERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSAK 60
           MENQNSEKMETTQKVERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSAK
Sbjct: 1   MENQNSEKMETTQKVERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSAK 60

Query: 61  GAIVLFLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPPH 120
           GAIVLFLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPPH
Sbjct: 61  GAIVLFLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPPH 120

Query: 121 CTPAGEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQSF 180
           CTPAGEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQSF
Sbjct: 121 CTPAGEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQSF 180

Query: 181 FNWYYASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMANK 240
           FNWYYASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMANK
Sbjct: 181 FNWYYASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMANK 240

Query: 241 SLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNIETDL 300
           SLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNIETDL
Sbjct: 241 SLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNIETDL 300

Query: 301 DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQFTFYSLQASTMDRHITP 360
           DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQFTFYSLQASTMDRHITP
Sbjct: 301 DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQFTFYSLQASTMDRHITP 360

Query: 361 HFQFPAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVISCLASI 420
           HFQFPAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVISCLASI
Sbjct: 361 HFQFPAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVISCLASI 420

Query: 421 VSAEIERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLP 480
           VSAEIERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLP
Sbjct: 421 VSAEIERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLP 480

Query: 481 RSMASIAVALFSLGMGGGSLVAALTVSVVKKRTKKNGKGWLSNNLNQGHYDYYYWVLSLM 540
           RSMASIAVALFSLGMGGGSLVAALTVSVVKKRTKKNGKGWLSNNLNQGHYDYYYWVLSLM
Sbjct: 481 RSMASIAVALFSLGMGGGSLVAALTVSVVKKRTKKNGKGWLSNNLNQGHYDYYYWVLSLM 540

Query: 541 GVANLLYYLICSWFYGDEKEGMEQSRIWDDKEAIEAEATFNS 583
           GVANLLYYLICSWFYGDEKEGMEQSRIWDDKEAIEAEATFNS
Sbjct: 541 GVANLLYYLICSWFYGDEKEGMEQSRIWDDKEAIEAEATFNS 582

BLAST of CsaV3_1G024440 vs. NCBI nr
Match: XP_008462682.1 (PREDICTED: protein NRT1/ PTR FAMILY 1.2-like [Cucumis melo] >KAA0046539.1 protein NRT1/ PTR FAMILY 1.2-like [Cucumis melo var. makuwa] >TYK28719.1 protein NRT1/ PTR FAMILY 1.2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1084.7 bits (2804), Expect = 0.0e+00
Identity = 550/583 (94.34%), Postives = 562/583 (96.40%), Query Frame = 0

Query: 1   MENQNSEKMETTQKVERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSAK 60
           MENQNSEKMETTQKVERKKGGLRTMPFIIANE FEKISNVGLHANMIFYLMNEYH+D AK
Sbjct: 1   MENQNSEKMETTQKVERKKGGLRTMPFIIANETFEKISNVGLHANMIFYLMNEYHMDKAK 60

Query: 61  GAIVLFLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPPH 120
           GAIVLFLWSALTN LPIGGAFLSDSYLGRFKVIAMGTVVTL+GMVVLWLTAIFPKARPPH
Sbjct: 61  GAIVLFLWSALTNFLPIGGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPPH 120

Query: 121 CTPAGEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQSF 180
           C P  EFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNP+NQRTMQSF
Sbjct: 121 CRPPSEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPKNQRTMQSF 180

Query: 181 FNWYYASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMANK 240
           FNWYYASVGISVTISVVFMVYLQNA GW+VGFGVPVGLMLFST MFFLGSSLYVKLMANK
Sbjct: 181 FNWYYASVGISVTISVVFMVYLQNAAGWIVGFGVPVGLMLFSTVMFFLGSSLYVKLMANK 240

Query: 241 SLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNIETDL 300
           SLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRN ETD+
Sbjct: 241 SLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETDV 300

Query: 301 DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQFTFYSLQASTMDRHITP 360
           DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGI+ISATINQFTF SLQASTMDRHITP
Sbjct: 301 DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIIISATINQFTFSSLQASTMDRHITP 360

Query: 361 HFQFPAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVISCLASI 420
           HFQFPAASFAVF ILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLV+SCL SI
Sbjct: 361 HFQFPAASFAVFAILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVVSCLTSI 420

Query: 421 VSAEIERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLP 480
           VSA IERKRRNRAIHEGLANVPGGIVQ+SAMWLVPQHCLAGLAEALNAIGQIQFFYSQLP
Sbjct: 421 VSAGIERKRRNRAIHEGLANVPGGIVQMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLP 480

Query: 481 RSMASIAVALFSLGMGGGSLVAALTVSVVKKRTKKNGK-GWLSNNLNQGHYDYYYWVLSL 540
           RSMASIAVALFSLGMGGGSLVAALTVSVVKKRT KNG  GWLS+NLNQGHYDYYYW+LSL
Sbjct: 481 RSMASIAVALFSLGMGGGSLVAALTVSVVKKRTTKNGNVGWLSDNLNQGHYDYYYWLLSL 540

Query: 541 MGVANLLYYLICSWFYGDEKEGMEQSRIWDDKEAIEAEATFNS 583
           MGVANLL+YLI SWFYGDEKEG EQSRIWDDKEAIE E T N+
Sbjct: 541 MGVANLLHYLIFSWFYGDEKEGEEQSRIWDDKEAIEVEGTSNA 583

BLAST of CsaV3_1G024440 vs. NCBI nr
Match: XP_038879889.1 (protein NRT1/ PTR FAMILY 1.2-like [Benincasa hispida])

HSP 1 Score: 1055.0 bits (2727), Expect = 2.3e-304
Identity = 533/580 (91.90%), Postives = 556/580 (95.86%), Query Frame = 0

Query: 1   MENQNSEK-METTQKVERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSA 60
           ME QNSE+ METTQKV RKKGGLRTMPFI+ANEIFEKISN+GLHANMIFYL+NEYHL SA
Sbjct: 1   MEKQNSEEVMETTQKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSA 60

Query: 61  KGAIVLFLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPP 120
           KGAIVLFLWSALTN LPI GAFLSDSYLGRFKVIAMGTVVTL+GMVVLWLTAIFPKARPP
Sbjct: 61  KGAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPP 120

Query: 121 HCTPAGEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQS 180
           HC P  EFCVSANTAQLMLLYFAFLLMA GAGGIRPCSLAFGADQLE PGNP+NQRTMQS
Sbjct: 121 HCKPPEEFCVSANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS 180

Query: 181 FFNWYYASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMAN 240
           FFNWYYA+VGISVTISVVFMVYLQNA GWVVG+GVPVGLM+ STAMFFLGSSLY+KLMAN
Sbjct: 181 FFNWYYATVGISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMAN 240

Query: 241 KSLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNIETD 300
           KSLLSSL QVIVAAWRNR+LEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRN ETD
Sbjct: 241 KSLLSSLAQVIVAAWRNRNLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETD 300

Query: 301 LDSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQFTFYSLQASTMDRHIT 360
           +DSNGMAKFPWRLSTIQRVEELKA+IR+LPIWSTGI+ISATINQFTF +LQA+TMDRHIT
Sbjct: 301 MDSNGMAKFPWRLSTIQRVEELKALIRLLPIWSTGIIISATINQFTFSALQANTMDRHIT 360

Query: 361 PHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVISCLAS 420
           PHFQFPAASFAVFTILTLTIWVAIYDQIIVPLL KFTKKSNGLTLKQRMGIGL ISCLAS
Sbjct: 361 PHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLAS 420

Query: 421 IVSAEIERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL 480
           +VSAEIERKRRNRAIHEGLANVP GIV++SAMWLVPQHCLAGLAEALNAIGQIQFFYSQL
Sbjct: 421 VVSAEIERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL 480

Query: 481 PRSMASIAVALFSLGMGGGSLVAALTVSVVKKRTKKNGK-GWLSNNLNQGHYDYYYWVLS 540
           PRSMASIAVA+FSLGMGGGSLVAAL VSVVKKRT KNG  GWLSNNLNQGHYDYYYWVLS
Sbjct: 481 PRSMASIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLS 540

Query: 541 LMGVANLLYYLICSWFYGDEKEGMEQSRIWDDKEAIEAEA 579
           LMGVANLLYYLICSWFYGDE+EGMEQSR+WDDKEAIE EA
Sbjct: 541 LMGVANLLYYLICSWFYGDEEEGMEQSRVWDDKEAIEEEA 580

BLAST of CsaV3_1G024440 vs. NCBI nr
Match: XP_022987664.1 (protein NRT1/ PTR FAMILY 1.2-like [Cucurbita maxima])

HSP 1 Score: 1005.7 bits (2599), Expect = 1.6e-289
Identity = 509/583 (87.31%), Postives = 535/583 (91.77%), Query Frame = 0

Query: 1   MENQNSEKMETTQKVERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSAK 60
           ME Q SEKMETTQK   KKGGLRTMPFI+ANEIFEKIS+ GLH NMIFYL NEYHLDSAK
Sbjct: 1   MEKQASEKMETTQKEAGKKGGLRTMPFIMANEIFEKISSFGLHPNMIFYLTNEYHLDSAK 60

Query: 61  GAIVLFLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPPH 120
            AIVLFLWSALTN LP  GAFLSDSYLGRFKVIAMGT+VTL+GMVVLWLTAIFPKARPPH
Sbjct: 61  AAIVLFLWSALTNFLPFVGAFLSDSYLGRFKVIAMGTLVTLLGMVVLWLTAIFPKARPPH 120

Query: 121 CTPAGEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQSF 180
           C   GEFCVSAN AQLMLLYFAFLLM+ GAGGIRPCSLAFGADQLE PGNP+NQRTMQSF
Sbjct: 121 CKSPGEFCVSANFAQLMLLYFAFLLMSIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQSF 180

Query: 181 FNWYYASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMANK 240
           FNWYYA+VG+SVTISV+FMVYLQNA GWVVG+GVPVGLMLFST MFFLGSSLYVKLMANK
Sbjct: 181 FNWYYATVGVSVTISVLFMVYLQNAAGWVVGYGVPVGLMLFSTVMFFLGSSLYVKLMANK 240

Query: 241 SLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNIETDL 300
           SLL SL QVIVAAW+NRHLE PPQ SDKWFYHKGSKL  PTPKLRFLNKAC+IRN ETD+
Sbjct: 241 SLLRSLAQVIVAAWKNRHLELPPQISDKWFYHKGSKLFIPTPKLRFLNKACMIRNKETDV 300

Query: 301 DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQFTFYSLQASTMDRHITP 360
           DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQ+TF +LQA TMDR ITP
Sbjct: 301 DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQYTFSALQAITMDRQITP 360

Query: 361 HFQFPAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVISCLASI 420
           HFQFPAASFAVFTILTLT WVAIYDQIIV LLAKFTK+SNGLT KQRMGIGL ISCLAS 
Sbjct: 361 HFQFPAASFAVFTILTLTFWVAIYDQIIVRLLAKFTKRSNGLTFKQRMGIGLAISCLASA 420

Query: 421 VSAEIERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLP 480
           VSAEIERKRRNRA HEGLANVPG IV++SAMWLVPQHCLAGLAEA +AIGQIQFFYSQLP
Sbjct: 421 VSAEIERKRRNRAFHEGLANVPGAIVKMSAMWLVPQHCLAGLAEAFHAIGQIQFFYSQLP 480

Query: 481 RSMASIAVALFSLGMGGGSLVAALTVSVVKKRTKKNGK-GWLSNNLNQGHYDYYYWVLSL 540
           RSMASIAVALFSLGMGGGSL+AAL VSVVKK+T KNG  GWLSNN NQGHYDYYYWVL+L
Sbjct: 481 RSMASIAVALFSLGMGGGSLLAALIVSVVKKKTTKNGNAGWLSNNPNQGHYDYYYWVLTL 540

Query: 541 MGVANLLYYLICSWFYGDEKEGMEQSRIWDDKEAIEAEATFNS 583
           MGVANL YYLICSW YGDEKE ME+SRIWDDKEAIE + T+N+
Sbjct: 541 MGVANLFYYLICSWLYGDEKEIMEESRIWDDKEAIEEDGTYNA 583

BLAST of CsaV3_1G024440 vs. NCBI nr
Match: KAG6589921.1 (Protein NRT1/ PTR FAMILY 1.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1005.4 bits (2598), Expect = 2.1e-289
Identity = 509/584 (87.16%), Postives = 539/584 (92.29%), Query Frame = 0

Query: 1   MENQNSEKMETTQKVERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSAK 60
           ME + SEKMETTQK   KKGGLRTMPFI+ANEIFEKIS+ GLH NMIFYL NEYHLDSAK
Sbjct: 1   MEKEPSEKMETTQKEAGKKGGLRTMPFIMANEIFEKISSFGLHTNMIFYLTNEYHLDSAK 60

Query: 61  GAIVLFLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPPH 120
           GAIVLFLWSALTN LPI GAFLSDSYLGRFKVIAMGT+VTL+GMVVLWLTAIFPKARPPH
Sbjct: 61  GAIVLFLWSALTNFLPIVGAFLSDSYLGRFKVIAMGTLVTLLGMVVLWLTAIFPKARPPH 120

Query: 121 CTPAGEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQSF 180
           C   GEFCVSAN AQLMLLYFAFLLM+ GAGGIRPCSLAFGADQLE PGNP+NQRTMQSF
Sbjct: 121 CKSPGEFCVSANFAQLMLLYFAFLLMSIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQSF 180

Query: 181 FNWYYASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMANK 240
           FNWYYA+VG+SVTISV+FMVYLQNA GWVVG+GVPVGLM+FST MFFLGSSLYVKLMANK
Sbjct: 181 FNWYYATVGVSVTISVLFMVYLQNAAGWVVGYGVPVGLMMFSTVMFFLGSSLYVKLMANK 240

Query: 241 SLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNIETDL 300
           SLL SL QVIVAAW+NRHLE PPQ SDKWFYHKGSKLV PTPKLRFLNKAC+IRN ETD+
Sbjct: 241 SLLRSLAQVIVAAWKNRHLELPPQTSDKWFYHKGSKLVNPTPKLRFLNKACMIRNKETDV 300

Query: 301 DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQFTFYSLQASTMDRHITP 360
           DSNGMAKFPWRL+TIQRVEELKAVIRVLPIWSTGIVISATI+Q+TF +LQA TMDR ITP
Sbjct: 301 DSNGMAKFPWRLTTIQRVEELKAVIRVLPIWSTGIVISATISQYTFSALQAITMDRQITP 360

Query: 361 HFQFPAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVISCLASI 420
           HFQFPAASFAVFTILTLT WVAIYDQIIV LLAKFTK+SNGLT KQRMGIGL ISCLAS 
Sbjct: 361 HFQFPAASFAVFTILTLTFWVAIYDQIIVRLLAKFTKRSNGLTFKQRMGIGLAISCLASA 420

Query: 421 VSAEIERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLP 480
           VSAEIERKRRNRA HEGLANVPGGIV++SAMWLVPQHCLAGLAEA +AIGQIQFFYSQLP
Sbjct: 421 VSAEIERKRRNRAFHEGLANVPGGIVKMSAMWLVPQHCLAGLAEAFHAIGQIQFFYSQLP 480

Query: 481 RSMASIAVALFSLGMGGGSLVAALTVSVVKKRTKKNGK-GWLSNNLNQGHYDYYYWVLSL 540
           RSMASIAVALFSLGMGGGSL+AAL VSVVKK+T KNG  GWLSNN NQGHYDYYYWVL+L
Sbjct: 481 RSMASIAVALFSLGMGGGSLLAALIVSVVKKKTTKNGNAGWLSNNPNQGHYDYYYWVLAL 540

Query: 541 MGVANLLYYLICSWFYGDEKEGMEQSRIW-DDKEAIEAEATFNS 583
           MGVANL YYLICSW YGDEKE ME+SRIW DDKEAIE + T+N+
Sbjct: 541 MGVANLFYYLICSWLYGDEKEIMEESRIWDDDKEAIEEDGTYNA 584

BLAST of CsaV3_1G024440 vs. ExPASy Swiss-Prot
Match: Q9M817 (Protein NRT1/ PTR FAMILY 1.2 OS=Arabidopsis thaliana OX=3702 GN=NPF1.2 PE=1 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 6.5e-161
Identity = 288/573 (50.26%), Postives = 401/573 (69.98%), Query Frame = 0

Query: 3   NQNSEKMETTQKVERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSAKGA 62
           N+   K   T + ++ KGG+ TMPFIIANE FEK+++ GL  NMI YL+ +Y    AKG 
Sbjct: 6   NETEAKQIQTNEGKKTKGGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYRFGVAKGT 65

Query: 63  IVLFLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPPHCT 122
            VLF+WSA +N  P+ GAFLSDSYLGRF  I++ ++ + +GMV+LWLTA+ P+ +P  C 
Sbjct: 66  NVLFMWSAASNFTPLLGAFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQVKPSPCD 125

Query: 123 P--AGEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQSF 182
           P  AG  C S+  +QL LLY AF L++ G+GGIRPCSLAFGADQL+N  NP+N+R ++SF
Sbjct: 126 PTAAGSHCGSSTASQLALLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESF 185

Query: 183 FNWYYASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMANK 242
           F WYYAS  ++V I+   +VY+Q   GW +GFGVP  LML +  +F L S LYV     K
Sbjct: 186 FGWYYASSAVAVLIAFTGIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLYVTRGVTK 245

Query: 243 SLLSSLTQVIVAAWRNRHLEFPP--QASDKWFYHKGSKLVTPTPKLRFLNKACIIRNIET 302
           SL + L Q IVAA++ R L  P    + D +++ K S++  P+ KLRFLNKAC+I N E 
Sbjct: 246 SLFTGLAQAIVAAYKKRKLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKACLISNREE 305

Query: 303 DLDSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQFTFYSLQASTMDRHI 362
           ++ S+G A  PWRL T  +VEELKA+I+V+PIWSTGI++S   +Q +F  LQA++MDR +
Sbjct: 306 EIGSDGFALNPWRLCTTDKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQATSMDRRL 365

Query: 363 TPH---FQFPAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVIS 422
           + H   FQ PA SF +FTI+ L +WV +YD+ ++PL +K   +   L++K RMG+GL +S
Sbjct: 366 SRHGSSFQVPAGSFGMFTIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRMGLGLFMS 425

Query: 423 CLASIVSAEIERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFF 482
            LA  +SA +E  RR +AI +G AN    +V +SAMWLVPQ+ L GLAEAL AIGQ +FF
Sbjct: 426 FLAMAISAMVESFRRKKAISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTAIGQTEFF 485

Query: 483 YSQLPRSMASIAVALFSLGMGGGSLVAALTVSVVKKRTKKNGK-GWLSNNLNQGHYDYYY 542
           Y++ P+SM+SIA +LF LGM   SL+A++ ++ V + T +NGK  W+S+N+N+GHY+YYY
Sbjct: 486 YTEFPKSMSSIAASLFGLGMAVASLLASVVLNAVNELTSRNGKESWVSDNINKGHYNYYY 545

Query: 543 WVLSLMGVANLLYYLICSWFYGDEKEGMEQSRI 568
           WVL++M   N++YY+ICSW YG   + +   R+
Sbjct: 546 WVLAIMSFINVIYYVICSWSYGPLVDQVRNGRV 578

BLAST of CsaV3_1G024440 vs. ExPASy Swiss-Prot
Match: Q8LPL2 (Protein NRT1/ PTR FAMILY 1.1 OS=Arabidopsis thaliana OX=3702 GN=NPF1.1 PE=1 SV=2)

HSP 1 Score: 556.2 bits (1432), Expect = 4.3e-157
Identity = 287/577 (49.74%), Postives = 398/577 (68.98%), Query Frame = 0

Query: 1   MEN--QNSEKMETTQKV---ERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYH 60
           MEN    +E  ET Q+     R KGGL TMPFIIANE FEK+++ GL  NMI YLM++Y 
Sbjct: 1   MENPPDQTESKETLQQPITRRRTKGGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYR 60

Query: 61  LDSAKGAIVLFLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPK 120
           L   KG  VLF+W A TN +P+ GAFLSDSYLGRF  I + ++ +L+GMVVLWLTA+ P+
Sbjct: 61  LGLVKGQTVLFMWVAATNFMPLVGAFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQ 120

Query: 121 ARPPHC-TPAGEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQ 180
            +P  C   AG  C SA ++QL LLY AF L++ G+GGIRPCSLAFGADQL+N  NP+N+
Sbjct: 121 VKPSPCVATAGTNCSSATSSQLALLYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNE 180

Query: 181 RTMQSFFNWYYASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYV 240
           R ++SFF WYYAS  ++V I+   +VY+Q+  GW +GFG+P  LML +  +F   S LYV
Sbjct: 181 RVLESFFGWYYASSSVAVLIAFTVIVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYV 240

Query: 241 KLMANKSLLSSLTQVIVAAWRNRHLEFPP--QASDKWFYHKGSKLVTPTPKLRFLNKACI 300
           K   +KSL + L QV+ AA+  R+L  P    + D ++  K S+L  P+ KLRFLNKAC 
Sbjct: 241 KRDVSKSLFTGLAQVVAAAYVKRNLTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACA 300

Query: 301 IRNIETDLDSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQFTFYSLQAS 360
           I N + DL S+G+A   WRL T  +VE+LKA+++V+P+WSTGI++S  ++Q +F  LQA 
Sbjct: 301 ISNRDEDLGSDGLALNQWRLCTTDQVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAK 360

Query: 361 TMDRHITPH--FQFPAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGI 420
           +MDR ++ +  FQ PA SF +FTI+ L  WV +YD+ I+PL +K   +   + +K RMG+
Sbjct: 361 SMDRRLSSNSTFQIPAGSFGMFTIIALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGL 420

Query: 421 GLVISCLASIVSAEIERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIG 480
           GL IS LA  VSA +E  RR  AI +GLAN     V +SAMWLVPQ+ L GLAEAL  IG
Sbjct: 421 GLFISFLAMAVSATVEHYRRKTAISQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIG 480

Query: 481 QIQFFYSQLPRSMASIAVALFSLGMGGGSLVAALTVSVVKKRTKKNGKGWLSNNLNQGHY 540
           Q +FFY++ P+SM+SIA +LF LGM   +++A++ ++ VK  +K+    W+ +N+N+GHY
Sbjct: 481 QTEFFYTEFPKSMSSIAASLFGLGMAVANILASVILNAVKNSSKQGNVSWIEDNINKGHY 540

Query: 541 DYYYWVLSLMGVANLLYYLICSWFYGDEKEGMEQSRI 568
           DYYYWVL+++   N++YY++CSW YG   + +   ++
Sbjct: 541 DYYYWVLAILSFVNVIYYVVCSWSYGPTVDQVRNDKV 577

BLAST of CsaV3_1G024440 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 2.9e-113
Identity = 246/570 (43.16%), Postives = 343/570 (60.18%), Query Frame = 0

Query: 1   MENQNSEKMETTQK-----VERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYH 60
           ME Q+  K+   +K       R KGGL TMPFI ANEI EK++ VG HANMI YL  + H
Sbjct: 1   MEEQSKNKISEEEKQLHGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLH 60

Query: 61  LDSAKGAIVLFLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPK 120
           L   K A  L  ++  ++L P+ GAF++DS+ GRF  I   +++  +GM +L ++AI P 
Sbjct: 61  LPLTKAANTLTNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPT 120

Query: 121 ARPPHCTPAGEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQR 180
            RPP C    E CV A+TAQL +LY A LL A G+GGIRPC +AFGADQ +   +P    
Sbjct: 121 LRPPPC-KGEEVCVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDE-SDPNQTT 180

Query: 181 TMQSFFNWYYASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVK 240
              ++FNWYY  +G +V ++V  +V++Q+  GW +G G+P   M  S   F  G  LY  
Sbjct: 181 KTWNYFNWYYFCMGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRH 240

Query: 241 LMANKSLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHK-------GSKLVTPTPKLRFLN 300
           L+   S  + L QV VAA+R R L      S  +F  +       G KL T T  + FL+
Sbjct: 241 LVPAGSPFTRLIQVGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGKL-THTKHMSFLD 300

Query: 301 KACIIRNIETDLDSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGI-VISATINQFTFY 360
           KA I+   E D    G     WRLST+ RVEELK+VIR+ PI ++GI +I+A   Q TF 
Sbjct: 301 KAAIV--TEEDNLKPGQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFS 360

Query: 361 SLQASTMDRHITPHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQR 420
             QA TM+RH+T  FQ PA S +VFT + +   +  YD++ V +  KFT    G+T   R
Sbjct: 361 LQQAKTMNRHLTNSFQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHR 420

Query: 421 MGIGLVISCLASIVSAEIERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALN 480
           MGIG VIS +A++V+  +E KR++ AI  GL + P  IV +S +WL+PQ+ L G+AEA  
Sbjct: 421 MGIGFVISIIATLVAGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFM 480

Query: 481 AIGQIQFFYSQLPRSMASIAVALFSLGMGGGSLVAALTVSVVKK-RTKKNGKGWL-SNNL 540
           +IG ++FFY Q P SM S A ALF + +  G+ V+ L V++V K   K +G  WL  NNL
Sbjct: 481 SIGHLEFFYDQAPESMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNL 540

Query: 541 NQGHYDYYYWVLSLMGVANLLYYLICSWFY 556
           N+G  +Y+YW+++++   NL+YYL C+  Y
Sbjct: 541 NRGRLEYFYWLITVLQAVNLVYYLWCAKIY 565

BLAST of CsaV3_1G024440 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 7.3e-112
Identity = 227/576 (39.41%), Postives = 342/576 (59.38%), Query Frame = 0

Query: 13  QKVERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSAKGAIVLFLWSALT 72
           +KVE+K GG R + FI+ NE  E++ ++GL AN + YL   +HL+    A V+ +WS  T
Sbjct: 46  EKVEKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFT 105

Query: 73  NLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPPHCTPAGEF-CVSA 132
           NL P+ GA++SD+Y+GRFK IA  +  TL+G++ + LTA FP+  P  C       C   
Sbjct: 106 NLTPLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGP 165

Query: 133 NTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQSFFNWYYASVGIS 192
           N  Q+ +L      ++ G+GGIRPCS+ FG DQ +        + + SFFNWYY +  + 
Sbjct: 166 NKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQ-RTEEGVKGVASFFNWYYMTFTVV 225

Query: 193 VTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMANKSLLSSLTQVIV 252
           + I+   +VY+Q+   W++GF +P GLM  +  MFF G   YV +    S+ S + QVIV
Sbjct: 226 LIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIV 285

Query: 253 AAWRNRHLEFPPQASDKWFYH----KGSKL--VTPTPKLRFLNKACIIRNIETDLDSNGM 312
           AA + R L+ P +      Y+    K S L  +  + + R L+KA ++  IE DL   G 
Sbjct: 286 AARKKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGP 345

Query: 313 AKFPWRLSTIQRVEELKAVIRVLPIWSTGIV-ISATINQFTFYSLQASTMDRHITPHFQF 372
               WRL ++Q VEE+K +IR++PIWS GI+ ++A   Q TF   QA  MDR++ P F+ 
Sbjct: 346 PADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEI 405

Query: 373 PAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVISCLASIVSAE 432
           PA S +V ++LT+ I++  YD++ VP + + T   +G+TL QR+G G+V +  + IV+  
Sbjct: 406 PAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGI 465

Query: 433 IERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLPRSMA 492
           +ER RR R+I+ G    P G+  +S  WL PQ  L GL EA N IGQI+FF SQ P  M 
Sbjct: 466 VERMRRIRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMR 525

Query: 493 SIAVALFSLGMGGGSLVAALTVSVVKKRTKKNGK-GWLSNNLNQGHYDYYYWVLSLMGVA 552
           SIA +LFSL   G S +++  V+VV K +  + +  WL+ NLN G  DY+Y++++++GV 
Sbjct: 526 SIANSLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVV 585

Query: 553 NLLYYLICSWFYGDEKEGMEQSRIWDDKEAIEAEAT 580
           NL+Y+  C+  Y   K G+      +DK + + E T
Sbjct: 586 NLVYFWYCARGY-RYKVGLPIEDFEEDKSSDDVEMT 614

BLAST of CsaV3_1G024440 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 371.7 bits (953), Expect = 1.5e-101
Identity = 217/572 (37.94%), Postives = 333/572 (58.22%), Query Frame = 0

Query: 16  ERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSAKGAIVLFLWSALTNLL 75
           E+K GG R + FI+ NE  EK+ ++G+ AN + YL N +H++  +   V +LW  LTN  
Sbjct: 12  EKKLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFA 71

Query: 76  PIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPPHC-TPAGEFCVSANTA 135
           P+ GA +SD+Y+GRFK IA  ++ +++G++ + LTA  P+  PP C  P  + C   N  
Sbjct: 72  PLLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKL 131

Query: 136 QLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQSFFNWYYASVGISVTI 195
           QL +L+     ++ G+GGIRPCS+ FG DQ +     +  + + SFFNWYY ++ + +  
Sbjct: 132 QLGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQ-RTEQGLKGVASFFNWYYLTLTMVLIF 191

Query: 196 SVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMANKSLLSSLTQVIVAAW 255
           S   +VYLQ  + WV+GF +P  LM  +  +FF+G   YV +    S+ S + +VIVAA 
Sbjct: 192 SHTVVVYLQTVS-WVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAAR 251

Query: 256 RNRHLEFPPQASDKWFYHKG-------SKLVTPTPKLRFLNKACIIRNIETDLDSNGMAK 315
           + R L+          Y++        SKL   T + +FL+KA +I  ++ DL S G+  
Sbjct: 252 KKRDLKISLVDDGTEEYYEPPVKPGVLSKLPL-TDQFKFLDKAAVI--LDGDLTSEGVPA 311

Query: 316 FPWRLSTIQRVEELKAVIRVLPIWSTGIV-ISATINQFTFYSLQASTMDRHITPHFQFPA 375
             WRL +IQ VEE+K +IRV+P+WS GI+ I A   Q TF   QA+ MDRH+ PHF+ PA
Sbjct: 312 NKWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPA 371

Query: 376 ASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVISCLASIVSAEIE 435
           AS  V + +T+ IWV IY+ ++VP L +  K    +TL QRMGIG+V + L+   +  +E
Sbjct: 372 ASITVISYITIGIWVPIYEHLLVPFLWRMRKFR--VTLLQRMGIGIVFAILSMFTAGFVE 431

Query: 436 RKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLPRSMASI 495
             RR RA           + Q+S  WL     L GL E+ N IG I+FF SQ P  M SI
Sbjct: 432 GVRRTRATE---------MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSI 491

Query: 496 AVALFSLGMGGGSLVAALTVSVVKKRT-KKNGKGWLSNNLNQGHYDYYYWVLSLMGVANL 555
           A +LF L     + +++L V+ V K +  K+   WL+ +L++G  DY+Y++++++GV NL
Sbjct: 492 ANSLFPLSFAAANYLSSLLVTTVHKVSGTKDHPDWLNKDLDRGKLDYFYYLIAVLGVVNL 551

Query: 556 LYYLICSWFYGDEKEGMEQSRIWDDKEAIEAE 578
           +Y+  C+  Y   K G +     ++K  ++ E
Sbjct: 552 VYFWYCAHRY-QYKAGSQIEDFNEEKSLLDIE 566

BLAST of CsaV3_1G024440 vs. ExPASy TrEMBL
Match: A0A5A7TZ76 (Protein NRT1/ PTR FAMILY 1.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00380 PE=3 SV=1)

HSP 1 Score: 1084.7 bits (2804), Expect = 0.0e+00
Identity = 550/583 (94.34%), Postives = 562/583 (96.40%), Query Frame = 0

Query: 1   MENQNSEKMETTQKVERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSAK 60
           MENQNSEKMETTQKVERKKGGLRTMPFIIANE FEKISNVGLHANMIFYLMNEYH+D AK
Sbjct: 1   MENQNSEKMETTQKVERKKGGLRTMPFIIANETFEKISNVGLHANMIFYLMNEYHMDKAK 60

Query: 61  GAIVLFLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPPH 120
           GAIVLFLWSALTN LPIGGAFLSDSYLGRFKVIAMGTVVTL+GMVVLWLTAIFPKARPPH
Sbjct: 61  GAIVLFLWSALTNFLPIGGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPPH 120

Query: 121 CTPAGEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQSF 180
           C P  EFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNP+NQRTMQSF
Sbjct: 121 CRPPSEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPKNQRTMQSF 180

Query: 181 FNWYYASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMANK 240
           FNWYYASVGISVTISVVFMVYLQNA GW+VGFGVPVGLMLFST MFFLGSSLYVKLMANK
Sbjct: 181 FNWYYASVGISVTISVVFMVYLQNAAGWIVGFGVPVGLMLFSTVMFFLGSSLYVKLMANK 240

Query: 241 SLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNIETDL 300
           SLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRN ETD+
Sbjct: 241 SLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETDV 300

Query: 301 DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQFTFYSLQASTMDRHITP 360
           DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGI+ISATINQFTF SLQASTMDRHITP
Sbjct: 301 DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIIISATINQFTFSSLQASTMDRHITP 360

Query: 361 HFQFPAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVISCLASI 420
           HFQFPAASFAVF ILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLV+SCL SI
Sbjct: 361 HFQFPAASFAVFAILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVVSCLTSI 420

Query: 421 VSAEIERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLP 480
           VSA IERKRRNRAIHEGLANVPGGIVQ+SAMWLVPQHCLAGLAEALNAIGQIQFFYSQLP
Sbjct: 421 VSAGIERKRRNRAIHEGLANVPGGIVQMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLP 480

Query: 481 RSMASIAVALFSLGMGGGSLVAALTVSVVKKRTKKNGK-GWLSNNLNQGHYDYYYWVLSL 540
           RSMASIAVALFSLGMGGGSLVAALTVSVVKKRT KNG  GWLS+NLNQGHYDYYYW+LSL
Sbjct: 481 RSMASIAVALFSLGMGGGSLVAALTVSVVKKRTTKNGNVGWLSDNLNQGHYDYYYWLLSL 540

Query: 541 MGVANLLYYLICSWFYGDEKEGMEQSRIWDDKEAIEAEATFNS 583
           MGVANLL+YLI SWFYGDEKEG EQSRIWDDKEAIE E T N+
Sbjct: 541 MGVANLLHYLIFSWFYGDEKEGEEQSRIWDDKEAIEVEGTSNA 583

BLAST of CsaV3_1G024440 vs. ExPASy TrEMBL
Match: A0A1S3CHG5 (protein NRT1/ PTR FAMILY 1.2-like OS=Cucumis melo OX=3656 GN=LOC103500984 PE=3 SV=1)

HSP 1 Score: 1084.7 bits (2804), Expect = 0.0e+00
Identity = 550/583 (94.34%), Postives = 562/583 (96.40%), Query Frame = 0

Query: 1   MENQNSEKMETTQKVERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSAK 60
           MENQNSEKMETTQKVERKKGGLRTMPFIIANE FEKISNVGLHANMIFYLMNEYH+D AK
Sbjct: 1   MENQNSEKMETTQKVERKKGGLRTMPFIIANETFEKISNVGLHANMIFYLMNEYHMDKAK 60

Query: 61  GAIVLFLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPPH 120
           GAIVLFLWSALTN LPIGGAFLSDSYLGRFKVIAMGTVVTL+GMVVLWLTAIFPKARPPH
Sbjct: 61  GAIVLFLWSALTNFLPIGGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPPH 120

Query: 121 CTPAGEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQSF 180
           C P  EFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNP+NQRTMQSF
Sbjct: 121 CRPPSEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPKNQRTMQSF 180

Query: 181 FNWYYASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMANK 240
           FNWYYASVGISVTISVVFMVYLQNA GW+VGFGVPVGLMLFST MFFLGSSLYVKLMANK
Sbjct: 181 FNWYYASVGISVTISVVFMVYLQNAAGWIVGFGVPVGLMLFSTVMFFLGSSLYVKLMANK 240

Query: 241 SLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNIETDL 300
           SLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRN ETD+
Sbjct: 241 SLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETDV 300

Query: 301 DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQFTFYSLQASTMDRHITP 360
           DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGI+ISATINQFTF SLQASTMDRHITP
Sbjct: 301 DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIIISATINQFTFSSLQASTMDRHITP 360

Query: 361 HFQFPAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVISCLASI 420
           HFQFPAASFAVF ILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLV+SCL SI
Sbjct: 361 HFQFPAASFAVFAILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVVSCLTSI 420

Query: 421 VSAEIERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLP 480
           VSA IERKRRNRAIHEGLANVPGGIVQ+SAMWLVPQHCLAGLAEALNAIGQIQFFYSQLP
Sbjct: 421 VSAGIERKRRNRAIHEGLANVPGGIVQMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLP 480

Query: 481 RSMASIAVALFSLGMGGGSLVAALTVSVVKKRTKKNGK-GWLSNNLNQGHYDYYYWVLSL 540
           RSMASIAVALFSLGMGGGSLVAALTVSVVKKRT KNG  GWLS+NLNQGHYDYYYW+LSL
Sbjct: 481 RSMASIAVALFSLGMGGGSLVAALTVSVVKKRTTKNGNVGWLSDNLNQGHYDYYYWLLSL 540

Query: 541 MGVANLLYYLICSWFYGDEKEGMEQSRIWDDKEAIEAEATFNS 583
           MGVANLL+YLI SWFYGDEKEG EQSRIWDDKEAIE E T N+
Sbjct: 541 MGVANLLHYLIFSWFYGDEKEGEEQSRIWDDKEAIEVEGTSNA 583

BLAST of CsaV3_1G024440 vs. ExPASy TrEMBL
Match: A0A6J1JJI0 (protein NRT1/ PTR FAMILY 1.2-like OS=Cucurbita maxima OX=3661 GN=LOC111485150 PE=3 SV=1)

HSP 1 Score: 1005.7 bits (2599), Expect = 7.7e-290
Identity = 509/583 (87.31%), Postives = 535/583 (91.77%), Query Frame = 0

Query: 1   MENQNSEKMETTQKVERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSAK 60
           ME Q SEKMETTQK   KKGGLRTMPFI+ANEIFEKIS+ GLH NMIFYL NEYHLDSAK
Sbjct: 1   MEKQASEKMETTQKEAGKKGGLRTMPFIMANEIFEKISSFGLHPNMIFYLTNEYHLDSAK 60

Query: 61  GAIVLFLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPPH 120
            AIVLFLWSALTN LP  GAFLSDSYLGRFKVIAMGT+VTL+GMVVLWLTAIFPKARPPH
Sbjct: 61  AAIVLFLWSALTNFLPFVGAFLSDSYLGRFKVIAMGTLVTLLGMVVLWLTAIFPKARPPH 120

Query: 121 CTPAGEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQSF 180
           C   GEFCVSAN AQLMLLYFAFLLM+ GAGGIRPCSLAFGADQLE PGNP+NQRTMQSF
Sbjct: 121 CKSPGEFCVSANFAQLMLLYFAFLLMSIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQSF 180

Query: 181 FNWYYASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMANK 240
           FNWYYA+VG+SVTISV+FMVYLQNA GWVVG+GVPVGLMLFST MFFLGSSLYVKLMANK
Sbjct: 181 FNWYYATVGVSVTISVLFMVYLQNAAGWVVGYGVPVGLMLFSTVMFFLGSSLYVKLMANK 240

Query: 241 SLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNIETDL 300
           SLL SL QVIVAAW+NRHLE PPQ SDKWFYHKGSKL  PTPKLRFLNKAC+IRN ETD+
Sbjct: 241 SLLRSLAQVIVAAWKNRHLELPPQISDKWFYHKGSKLFIPTPKLRFLNKACMIRNKETDV 300

Query: 301 DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQFTFYSLQASTMDRHITP 360
           DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQ+TF +LQA TMDR ITP
Sbjct: 301 DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQYTFSALQAITMDRQITP 360

Query: 361 HFQFPAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVISCLASI 420
           HFQFPAASFAVFTILTLT WVAIYDQIIV LLAKFTK+SNGLT KQRMGIGL ISCLAS 
Sbjct: 361 HFQFPAASFAVFTILTLTFWVAIYDQIIVRLLAKFTKRSNGLTFKQRMGIGLAISCLASA 420

Query: 421 VSAEIERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLP 480
           VSAEIERKRRNRA HEGLANVPG IV++SAMWLVPQHCLAGLAEA +AIGQIQFFYSQLP
Sbjct: 421 VSAEIERKRRNRAFHEGLANVPGAIVKMSAMWLVPQHCLAGLAEAFHAIGQIQFFYSQLP 480

Query: 481 RSMASIAVALFSLGMGGGSLVAALTVSVVKKRTKKNGK-GWLSNNLNQGHYDYYYWVLSL 540
           RSMASIAVALFSLGMGGGSL+AAL VSVVKK+T KNG  GWLSNN NQGHYDYYYWVL+L
Sbjct: 481 RSMASIAVALFSLGMGGGSLLAALIVSVVKKKTTKNGNAGWLSNNPNQGHYDYYYWVLTL 540

Query: 541 MGVANLLYYLICSWFYGDEKEGMEQSRIWDDKEAIEAEATFNS 583
           MGVANL YYLICSW YGDEKE ME+SRIWDDKEAIE + T+N+
Sbjct: 541 MGVANLFYYLICSWLYGDEKEIMEESRIWDDKEAIEEDGTYNA 583

BLAST of CsaV3_1G024440 vs. ExPASy TrEMBL
Match: A0A6J1HA09 (protein NRT1/ PTR FAMILY 1.1-like OS=Cucurbita moschata OX=3662 GN=LOC111461844 PE=3 SV=1)

HSP 1 Score: 1002.7 bits (2591), Expect = 6.5e-289
Identity = 508/584 (86.99%), Postives = 538/584 (92.12%), Query Frame = 0

Query: 1   MENQNSEKMETTQKVERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSAK 60
           M  + SEKMETTQK   KKGGLRTMPFI+ANEIFEKIS+ GLH NMIFYL NEYHLDSAK
Sbjct: 36  MGKEPSEKMETTQKEAGKKGGLRTMPFIMANEIFEKISSFGLHTNMIFYLTNEYHLDSAK 95

Query: 61  GAIVLFLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPPH 120
           GAIVLFLWSALTN LPI GAFLSDSYLGRFKVIAMGT+VTL+GMVVLWLTAIFPKARPPH
Sbjct: 96  GAIVLFLWSALTNFLPIVGAFLSDSYLGRFKVIAMGTLVTLLGMVVLWLTAIFPKARPPH 155

Query: 121 CTPAGEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQSF 180
           C   GEFCVSAN AQLMLLYFAFLLM+ GAGGIRPCSLAFGADQLE PGNP+NQRTMQSF
Sbjct: 156 CKSPGEFCVSANFAQLMLLYFAFLLMSIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQSF 215

Query: 181 FNWYYASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMANK 240
           FNWYYA+VG+SVTISV+FMVYLQNA GWVVG+GVPVGLM+FST MFFLGSSLYVKLMANK
Sbjct: 216 FNWYYATVGVSVTISVLFMVYLQNAAGWVVGYGVPVGLMMFSTVMFFLGSSLYVKLMANK 275

Query: 241 SLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNIETDL 300
           SLL SL QVIVAAW+NRHLE PPQ SDKWFYHKGSKLV PTPKLRFLNKAC+IRN ETD+
Sbjct: 276 SLLRSLAQVIVAAWKNRHLELPPQTSDKWFYHKGSKLVNPTPKLRFLNKACMIRNKETDV 335

Query: 301 DSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQFTFYSLQASTMDRHITP 360
           DSNGMAKFPWRL+TIQRVEELKAVIRVLPIWSTGIVISATI+Q+TF +LQA TMDR ITP
Sbjct: 336 DSNGMAKFPWRLTTIQRVEELKAVIRVLPIWSTGIVISATISQYTFSALQAITMDRQITP 395

Query: 361 HFQFPAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVISCLASI 420
           HFQFPAASFAVFTILTLT WVAIYDQIIV LLAKFTK+SNGLT KQRMGIGL ISCLAS 
Sbjct: 396 HFQFPAASFAVFTILTLTFWVAIYDQIIVRLLAKFTKRSNGLTFKQRMGIGLAISCLASA 455

Query: 421 VSAEIERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLP 480
           VSAEIERKRRNRA HEGLANVPGGIV++SAMWLVPQHCLAGLAEA +AIGQIQFFYSQLP
Sbjct: 456 VSAEIERKRRNRAFHEGLANVPGGIVKMSAMWLVPQHCLAGLAEAFHAIGQIQFFYSQLP 515

Query: 481 RSMASIAVALFSLGMGGGSLVAALTVSVVKKRTKKNGK-GWLSNNLNQGHYDYYYWVLSL 540
           RSMASIAVALFSLGMGGGSL+AAL VSVVKK+T KNG  GWLSNN NQGHYDYYYWVL+L
Sbjct: 516 RSMASIAVALFSLGMGGGSLLAALIVSVVKKKTTKNGNAGWLSNNPNQGHYDYYYWVLAL 575

Query: 541 MGVANLLYYLICSWFYGDEKEGMEQSRIW-DDKEAIEAEATFNS 583
           MGVANL YYLICSW YGDEKE ME+SRIW DDKEAIE + T+N+
Sbjct: 576 MGVANLFYYLICSWLYGDEKEIMEESRIWDDDKEAIEEDGTYNA 619

BLAST of CsaV3_1G024440 vs. ExPASy TrEMBL
Match: A0A6J1CUG1 (protein NRT1/ PTR FAMILY 1.2-like OS=Momordica charantia OX=3673 GN=LOC111014453 PE=3 SV=1)

HSP 1 Score: 980.3 bits (2533), Expect = 3.4e-282
Identity = 495/578 (85.64%), Postives = 528/578 (91.35%), Query Frame = 0

Query: 6   SEKMETTQKVERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSAKGAIVL 65
           SEKME TQKV RKKGGLRTMPFI+ANEIFEKIS++GLHANMIFYL NEYH DSAKGAIVL
Sbjct: 5   SEKMEATQKVGRKKGGLRTMPFIMANEIFEKISSIGLHANMIFYLTNEYHFDSAKGAIVL 64

Query: 66  FLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPPHCTPAG 125
           FLWSALTN LPI GAFLSDSYLGRFKVI+MGTVV+L+GMVVL LTAIFPKARPPHC    
Sbjct: 65  FLWSALTNFLPIAGAFLSDSYLGRFKVISMGTVVSLLGMVVLLLTAIFPKARPPHCKLPP 124

Query: 126 EFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQSFFNWYY 185
           E CV AN  QL+LL+F+FLLMA GAGGIRPCSLAFGADQ E PGNP+N+RT+QSFFNWYY
Sbjct: 125 EICVHANGGQLLLLFFSFLLMAIGAGGIRPCSLAFGADQFEKPGNPKNERTLQSFFNWYY 184

Query: 186 ASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMANKSLLSS 245
           A+VG+SVTISV FMVYLQNA GWVVG+GVPVGLMLFST MFFLGSSLYVK+ ANKSLL S
Sbjct: 185 ATVGVSVTISVTFMVYLQNAAGWVVGYGVPVGLMLFSTIMFFLGSSLYVKIKANKSLLGS 244

Query: 246 LTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNIETDLDSNGM 305
           L QVIVAAWRNRHL+ PPQ SDKWFYHKGSKLV+PTPKLRFLNKACIIRN ETD+D+NGM
Sbjct: 245 LAQVIVAAWRNRHLDSPPQTSDKWFYHKGSKLVSPTPKLRFLNKACIIRNRETDVDANGM 304

Query: 306 AKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQFTFYSLQASTMDRHITPHFQFP 365
           A+FPW LSTIQRVEELKAVIRVLPIWSTGIVISA I QFTF +LQASTMDRHITP FQFP
Sbjct: 305 ARFPWSLSTIQRVEELKAVIRVLPIWSTGIVISAAITQFTFAALQASTMDRHITPRFQFP 364

Query: 366 AASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVISCLASIVSAEI 425
           AASFAVFTI+TLTIWVAIYDQIIV LLAKFTK SNGL+LKQRMGIGL ISCLAS VSAEI
Sbjct: 365 AASFAVFTIITLTIWVAIYDQIIVRLLAKFTKLSNGLSLKQRMGIGLAISCLASAVSAEI 424

Query: 426 ERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLPRSMAS 485
           ER RRNRAI EGL NVP GIV++SAMWLVPQHCLAGLAEALNAIGQIQFFYSQLPRSMAS
Sbjct: 425 ERTRRNRAIDEGLGNVPNGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLPRSMAS 484

Query: 486 IAVALFSLGMGGGSLVAALTVSVVKKRTKKNGK-GWLSNNLNQGHYDYYYWVLSLMGVAN 545
           IAVALFSLGMGGGSL+ AL VSV+KK T+KNG  GWLSNNLNQGHYDYYYWVLS+MGV N
Sbjct: 485 IAVALFSLGMGGGSLLGALVVSVIKKTTEKNGNVGWLSNNLNQGHYDYYYWVLSMMGVVN 544

Query: 546 LLYYLICSWFYGDEKEGMEQSRIWDDKEAIEAEATFNS 583
            LYYLICSWFYGDEKEGME SR+WD+KEAIE EA FN+
Sbjct: 545 FLYYLICSWFYGDEKEGMEASRVWDEKEAIE-EAPFNA 581

BLAST of CsaV3_1G024440 vs. TAIR 10
Match: AT1G52190.1 (Major facilitator superfamily protein )

HSP 1 Score: 568.9 bits (1465), Expect = 4.6e-162
Identity = 288/573 (50.26%), Postives = 401/573 (69.98%), Query Frame = 0

Query: 3   NQNSEKMETTQKVERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSAKGA 62
           N+   K   T + ++ KGG+ TMPFIIANE FEK+++ GL  NMI YL+ +Y    AKG 
Sbjct: 6   NETEAKQIQTNEGKKTKGGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYRFGVAKGT 65

Query: 63  IVLFLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPPHCT 122
            VLF+WSA +N  P+ GAFLSDSYLGRF  I++ ++ + +GMV+LWLTA+ P+ +P  C 
Sbjct: 66  NVLFMWSAASNFTPLLGAFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQVKPSPCD 125

Query: 123 P--AGEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQSF 182
           P  AG  C S+  +QL LLY AF L++ G+GGIRPCSLAFGADQL+N  NP+N+R ++SF
Sbjct: 126 PTAAGSHCGSSTASQLALLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESF 185

Query: 183 FNWYYASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMANK 242
           F WYYAS  ++V I+   +VY+Q   GW +GFGVP  LML +  +F L S LYV     K
Sbjct: 186 FGWYYASSAVAVLIAFTGIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLYVTRGVTK 245

Query: 243 SLLSSLTQVIVAAWRNRHLEFPP--QASDKWFYHKGSKLVTPTPKLRFLNKACIIRNIET 302
           SL + L Q IVAA++ R L  P    + D +++ K S++  P+ KLRFLNKAC+I N E 
Sbjct: 246 SLFTGLAQAIVAAYKKRKLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKACLISNREE 305

Query: 303 DLDSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQFTFYSLQASTMDRHI 362
           ++ S+G A  PWRL T  +VEELKA+I+V+PIWSTGI++S   +Q +F  LQA++MDR +
Sbjct: 306 EIGSDGFALNPWRLCTTDKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQATSMDRRL 365

Query: 363 TPH---FQFPAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVIS 422
           + H   FQ PA SF +FTI+ L +WV +YD+ ++PL +K   +   L++K RMG+GL +S
Sbjct: 366 SRHGSSFQVPAGSFGMFTIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRMGLGLFMS 425

Query: 423 CLASIVSAEIERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFF 482
            LA  +SA +E  RR +AI +G AN    +V +SAMWLVPQ+ L GLAEAL AIGQ +FF
Sbjct: 426 FLAMAISAMVESFRRKKAISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTAIGQTEFF 485

Query: 483 YSQLPRSMASIAVALFSLGMGGGSLVAALTVSVVKKRTKKNGK-GWLSNNLNQGHYDYYY 542
           Y++ P+SM+SIA +LF LGM   SL+A++ ++ V + T +NGK  W+S+N+N+GHY+YYY
Sbjct: 486 YTEFPKSMSSIAASLFGLGMAVASLLASVVLNAVNELTSRNGKESWVSDNINKGHYNYYY 545

Query: 543 WVLSLMGVANLLYYLICSWFYGDEKEGMEQSRI 568
           WVL++M   N++YY+ICSW YG   + +   R+
Sbjct: 546 WVLAIMSFINVIYYVICSWSYGPLVDQVRNGRV 578

BLAST of CsaV3_1G024440 vs. TAIR 10
Match: AT3G16180.1 (Major facilitator superfamily protein )

HSP 1 Score: 556.2 bits (1432), Expect = 3.1e-158
Identity = 287/577 (49.74%), Postives = 398/577 (68.98%), Query Frame = 0

Query: 1   MEN--QNSEKMETTQKV---ERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYH 60
           MEN    +E  ET Q+     R KGGL TMPFIIANE FEK+++ GL  NMI YLM++Y 
Sbjct: 1   MENPPDQTESKETLQQPITRRRTKGGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYR 60

Query: 61  LDSAKGAIVLFLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPK 120
           L   KG  VLF+W A TN +P+ GAFLSDSYLGRF  I + ++ +L+GMVVLWLTA+ P+
Sbjct: 61  LGLVKGQTVLFMWVAATNFMPLVGAFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQ 120

Query: 121 ARPPHC-TPAGEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQ 180
            +P  C   AG  C SA ++QL LLY AF L++ G+GGIRPCSLAFGADQL+N  NP+N+
Sbjct: 121 VKPSPCVATAGTNCSSATSSQLALLYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNE 180

Query: 181 RTMQSFFNWYYASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYV 240
           R ++SFF WYYAS  ++V I+   +VY+Q+  GW +GFG+P  LML +  +F   S LYV
Sbjct: 181 RVLESFFGWYYASSSVAVLIAFTVIVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYV 240

Query: 241 KLMANKSLLSSLTQVIVAAWRNRHLEFPP--QASDKWFYHKGSKLVTPTPKLRFLNKACI 300
           K   +KSL + L QV+ AA+  R+L  P    + D ++  K S+L  P+ KLRFLNKAC 
Sbjct: 241 KRDVSKSLFTGLAQVVAAAYVKRNLTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACA 300

Query: 301 IRNIETDLDSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQFTFYSLQAS 360
           I N + DL S+G+A   WRL T  +VE+LKA+++V+P+WSTGI++S  ++Q +F  LQA 
Sbjct: 301 ISNRDEDLGSDGLALNQWRLCTTDQVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAK 360

Query: 361 TMDRHITPH--FQFPAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGI 420
           +MDR ++ +  FQ PA SF +FTI+ L  WV +YD+ I+PL +K   +   + +K RMG+
Sbjct: 361 SMDRRLSSNSTFQIPAGSFGMFTIIALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGL 420

Query: 421 GLVISCLASIVSAEIERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIG 480
           GL IS LA  VSA +E  RR  AI +GLAN     V +SAMWLVPQ+ L GLAEAL  IG
Sbjct: 421 GLFISFLAMAVSATVEHYRRKTAISQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIG 480

Query: 481 QIQFFYSQLPRSMASIAVALFSLGMGGGSLVAALTVSVVKKRTKKNGKGWLSNNLNQGHY 540
           Q +FFY++ P+SM+SIA +LF LGM   +++A++ ++ VK  +K+    W+ +N+N+GHY
Sbjct: 481 QTEFFYTEFPKSMSSIAASLFGLGMAVANILASVILNAVKNSSKQGNVSWIEDNINKGHY 540

Query: 541 DYYYWVLSLMGVANLLYYLICSWFYGDEKEGMEQSRI 568
           DYYYWVL+++   N++YY++CSW YG   + +   ++
Sbjct: 541 DYYYWVLAILSFVNVIYYVVCSWSYGPTVDQVRNDKV 577

BLAST of CsaV3_1G024440 vs. TAIR 10
Match: AT1G68570.1 (Major facilitator superfamily protein )

HSP 1 Score: 410.6 bits (1054), Expect = 2.1e-114
Identity = 246/570 (43.16%), Postives = 343/570 (60.18%), Query Frame = 0

Query: 1   MENQNSEKMETTQK-----VERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYH 60
           ME Q+  K+   +K       R KGGL TMPFI ANEI EK++ VG HANMI YL  + H
Sbjct: 1   MEEQSKNKISEEEKQLHGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLH 60

Query: 61  LDSAKGAIVLFLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPK 120
           L   K A  L  ++  ++L P+ GAF++DS+ GRF  I   +++  +GM +L ++AI P 
Sbjct: 61  LPLTKAANTLTNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPT 120

Query: 121 ARPPHCTPAGEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQR 180
            RPP C    E CV A+TAQL +LY A LL A G+GGIRPC +AFGADQ +   +P    
Sbjct: 121 LRPPPC-KGEEVCVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDE-SDPNQTT 180

Query: 181 TMQSFFNWYYASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVK 240
              ++FNWYY  +G +V ++V  +V++Q+  GW +G G+P   M  S   F  G  LY  
Sbjct: 181 KTWNYFNWYYFCMGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRH 240

Query: 241 LMANKSLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHK-------GSKLVTPTPKLRFLN 300
           L+   S  + L QV VAA+R R L      S  +F  +       G KL T T  + FL+
Sbjct: 241 LVPAGSPFTRLIQVGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGKL-THTKHMSFLD 300

Query: 301 KACIIRNIETDLDSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGI-VISATINQFTFY 360
           KA I+   E D    G     WRLST+ RVEELK+VIR+ PI ++GI +I+A   Q TF 
Sbjct: 301 KAAIV--TEEDNLKPGQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFS 360

Query: 361 SLQASTMDRHITPHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQR 420
             QA TM+RH+T  FQ PA S +VFT + +   +  YD++ V +  KFT    G+T   R
Sbjct: 361 LQQAKTMNRHLTNSFQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHR 420

Query: 421 MGIGLVISCLASIVSAEIERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALN 480
           MGIG VIS +A++V+  +E KR++ AI  GL + P  IV +S +WL+PQ+ L G+AEA  
Sbjct: 421 MGIGFVISIIATLVAGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFM 480

Query: 481 AIGQIQFFYSQLPRSMASIAVALFSLGMGGGSLVAALTVSVVKK-RTKKNGKGWL-SNNL 540
           +IG ++FFY Q P SM S A ALF + +  G+ V+ L V++V K   K +G  WL  NNL
Sbjct: 481 SIGHLEFFYDQAPESMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNL 540

Query: 541 NQGHYDYYYWVLSLMGVANLLYYLICSWFY 556
           N+G  +Y+YW+++++   NL+YYL C+  Y
Sbjct: 541 NRGRLEYFYWLITVLQAVNLVYYLWCAKIY 565

BLAST of CsaV3_1G024440 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 406.0 bits (1042), Expect = 5.2e-113
Identity = 227/576 (39.41%), Postives = 342/576 (59.38%), Query Frame = 0

Query: 13  QKVERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSAKGAIVLFLWSALT 72
           +KVE+K GG R + FI+ NE  E++ ++GL AN + YL   +HL+    A V+ +WS  T
Sbjct: 46  EKVEKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFT 105

Query: 73  NLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPPHCTPAGEF-CVSA 132
           NL P+ GA++SD+Y+GRFK IA  +  TL+G++ + LTA FP+  P  C       C   
Sbjct: 106 NLTPLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGP 165

Query: 133 NTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQSFFNWYYASVGIS 192
           N  Q+ +L      ++ G+GGIRPCS+ FG DQ +        + + SFFNWYY +  + 
Sbjct: 166 NKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQ-RTEEGVKGVASFFNWYYMTFTVV 225

Query: 193 VTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMANKSLLSSLTQVIV 252
           + I+   +VY+Q+   W++GF +P GLM  +  MFF G   YV +    S+ S + QVIV
Sbjct: 226 LIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIV 285

Query: 253 AAWRNRHLEFPPQASDKWFYH----KGSKL--VTPTPKLRFLNKACIIRNIETDLDSNGM 312
           AA + R L+ P +      Y+    K S L  +  + + R L+KA ++  IE DL   G 
Sbjct: 286 AARKKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGP 345

Query: 313 AKFPWRLSTIQRVEELKAVIRVLPIWSTGIV-ISATINQFTFYSLQASTMDRHITPHFQF 372
               WRL ++Q VEE+K +IR++PIWS GI+ ++A   Q TF   QA  MDR++ P F+ 
Sbjct: 346 PADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEI 405

Query: 373 PAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVISCLASIVSAE 432
           PA S +V ++LT+ I++  YD++ VP + + T   +G+TL QR+G G+V +  + IV+  
Sbjct: 406 PAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGI 465

Query: 433 IERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLPRSMA 492
           +ER RR R+I+ G    P G+  +S  WL PQ  L GL EA N IGQI+FF SQ P  M 
Sbjct: 466 VERMRRIRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMR 525

Query: 493 SIAVALFSLGMGGGSLVAALTVSVVKKRTKKNGK-GWLSNNLNQGHYDYYYWVLSLMGVA 552
           SIA +LFSL   G S +++  V+VV K +  + +  WL+ NLN G  DY+Y++++++GV 
Sbjct: 526 SIANSLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVV 585

Query: 553 NLLYYLICSWFYGDEKEGMEQSRIWDDKEAIEAEAT 580
           NL+Y+  C+  Y   K G+      +DK + + E T
Sbjct: 586 NLVYFWYCARGY-RYKVGLPIEDFEEDKSSDDVEMT 614

BLAST of CsaV3_1G024440 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 371.7 bits (953), Expect = 1.1e-102
Identity = 217/572 (37.94%), Postives = 333/572 (58.22%), Query Frame = 0

Query: 16  ERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSAKGAIVLFLWSALTNLL 75
           E+K GG R + FI+ NE  EK+ ++G+ AN + YL N +H++  +   V +LW  LTN  
Sbjct: 12  EKKLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFA 71

Query: 76  PIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPPHC-TPAGEFCVSANTA 135
           P+ GA +SD+Y+GRFK IA  ++ +++G++ + LTA  P+  PP C  P  + C   N  
Sbjct: 72  PLLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKL 131

Query: 136 QLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQSFFNWYYASVGISVTI 195
           QL +L+     ++ G+GGIRPCS+ FG DQ +     +  + + SFFNWYY ++ + +  
Sbjct: 132 QLGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQ-RTEQGLKGVASFFNWYYLTLTMVLIF 191

Query: 196 SVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMANKSLLSSLTQVIVAAW 255
           S   +VYLQ  + WV+GF +P  LM  +  +FF+G   YV +    S+ S + +VIVAA 
Sbjct: 192 SHTVVVYLQTVS-WVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAAR 251

Query: 256 RNRHLEFPPQASDKWFYHKG-------SKLVTPTPKLRFLNKACIIRNIETDLDSNGMAK 315
           + R L+          Y++        SKL   T + +FL+KA +I  ++ DL S G+  
Sbjct: 252 KKRDLKISLVDDGTEEYYEPPVKPGVLSKLPL-TDQFKFLDKAAVI--LDGDLTSEGVPA 311

Query: 316 FPWRLSTIQRVEELKAVIRVLPIWSTGIV-ISATINQFTFYSLQASTMDRHITPHFQFPA 375
             WRL +IQ VEE+K +IRV+P+WS GI+ I A   Q TF   QA+ MDRH+ PHF+ PA
Sbjct: 312 NKWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPA 371

Query: 376 ASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVISCLASIVSAEIE 435
           AS  V + +T+ IWV IY+ ++VP L +  K    +TL QRMGIG+V + L+   +  +E
Sbjct: 372 ASITVISYITIGIWVPIYEHLLVPFLWRMRKFR--VTLLQRMGIGIVFAILSMFTAGFVE 431

Query: 436 RKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLPRSMASI 495
             RR RA           + Q+S  WL     L GL E+ N IG I+FF SQ P  M SI
Sbjct: 432 GVRRTRATE---------MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSI 491

Query: 496 AVALFSLGMGGGSLVAALTVSVVKKRT-KKNGKGWLSNNLNQGHYDYYYWVLSLMGVANL 555
           A +LF L     + +++L V+ V K +  K+   WL+ +L++G  DY+Y++++++GV NL
Sbjct: 492 ANSLFPLSFAAANYLSSLLVTTVHKVSGTKDHPDWLNKDLDRGKLDYFYYLIAVLGVVNL 551

Query: 556 LYYLICSWFYGDEKEGMEQSRIWDDKEAIEAE 578
           +Y+  C+  Y   K G +     ++K  ++ E
Sbjct: 552 VYFWYCAHRY-QYKAGSQIEDFNEEKSLLDIE 566

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAE8652969.10.0e+00100.00hypothetical protein Csa_017739 [Cucumis sativus][more]
XP_008462682.10.0e+0094.34PREDICTED: protein NRT1/ PTR FAMILY 1.2-like [Cucumis melo] >KAA0046539.1 protei... [more]
XP_038879889.12.3e-30491.90protein NRT1/ PTR FAMILY 1.2-like [Benincasa hispida][more]
XP_022987664.11.6e-28987.31protein NRT1/ PTR FAMILY 1.2-like [Cucurbita maxima][more]
KAG6589921.12.1e-28987.16Protein NRT1/ PTR FAMILY 1.2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9M8176.5e-16150.26Protein NRT1/ PTR FAMILY 1.2 OS=Arabidopsis thaliana OX=3702 GN=NPF1.2 PE=1 SV=1[more]
Q8LPL24.3e-15749.74Protein NRT1/ PTR FAMILY 1.1 OS=Arabidopsis thaliana OX=3702 GN=NPF1.1 PE=1 SV=2[more]
Q9SX202.9e-11343.16Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1[more]
Q8RX777.3e-11239.41Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV... [more]
Q9LFX91.5e-10137.94Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A5A7TZ760.0e+0094.34Protein NRT1/ PTR FAMILY 1.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3CHG50.0e+0094.34protein NRT1/ PTR FAMILY 1.2-like OS=Cucumis melo OX=3656 GN=LOC103500984 PE=3 S... [more]
A0A6J1JJI07.7e-29087.31protein NRT1/ PTR FAMILY 1.2-like OS=Cucurbita maxima OX=3661 GN=LOC111485150 PE... [more]
A0A6J1HA096.5e-28986.99protein NRT1/ PTR FAMILY 1.1-like OS=Cucurbita moschata OX=3662 GN=LOC111461844 ... [more]
A0A6J1CUG13.4e-28285.64protein NRT1/ PTR FAMILY 1.2-like OS=Momordica charantia OX=3673 GN=LOC111014453... [more]
Match NameE-valueIdentityDescription
AT1G52190.14.6e-16250.26Major facilitator superfamily protein [more]
AT3G16180.13.1e-15849.74Major facilitator superfamily protein [more]
AT1G68570.12.1e-11443.16Major facilitator superfamily protein [more]
AT1G69870.15.2e-11339.41nitrate transporter 1.7 [more]
AT1G27080.11.1e-10237.94nitrate transporter 1.6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..564
e-value: 7.5E-157
score: 524.7
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 44..552
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 90..521
e-value: 7.9E-73
score: 245.6
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 15..562
NoneNo IPR availablePANTHERPTHR11654:SF150OS01G0761500 PROTEINcoord: 15..562
NoneNo IPR availableCDDcd17416MFS_NPF1_2coord: 25..555
e-value: 0.0
score: 534.923
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 79..103

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_1G024440.1CsaV3_1G024440.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity