CsaV3_1G015110 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_1G015110
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionNitrilase/cyanide hydratase
Locationchr1: 10725091 .. 10730232 (-)
RNA-Seq ExpressionCsaV3_1G015110
SyntenyCsaV3_1G015110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CACAAATTTCCACCTCCACTCGAAAACTTAGTTTCTAAGTTTTCTCTCCTCATCCACTTGCATTCGCAAATCTCGCCATTCCGTTTGGACGATACTATCATCATCGTTTGTATTAATCAAGCAACTTGACCATCCTGAATAATGAAGTGAATATATATATATATCCTTGTTGCTTCTTCTAGCAACTTCCACAAATTTCATCTATCTACAACGAACGGAGATAAATAATAAAAAGTGTAAAACTCGAGTCCATACATTGCGTTGCCATTGATAGCCCTGTTGCTCGCCGGCTGAAGCTCAAGCTGAGAGAGAGAGAAAAAAAAAAGGGAGATTCAAGCAGCAACCATGGCGCTTGTGCCAACCACGGCCCCTACCGACTCCCAACTGATAGCGGAGGTTGACATGGGCTCCGATTCATCTGTAATGAATGTTCGAGCCACCGTCGTTCAGGCCTCCACCATCTTCTATGACACTCCGGCCACTCTAGGTTCCTTTTCCTTGCTTCTATTTTCAATTTACTCCTTATGTACTACTCTTGCTCTCGCTGTTGCTTTGCTCACTAGCTCTTGGATCGCAATGGTCAGTTCAGAGTTTGGAATGAGTTCCAGATTGTTTTTGCTTGTGTTCTTCGAAGCAAGGTTATTAGTTGTTTTGGCTGTTTTTTTGCTTGGTTTTCTTTAATCTTACAAGTTGCTTTTCTTTCATCAGCTGATTTTGGTTGAATAATTTAATTAGTTTAAACTATTACATCCTCGATTACTTTTCCTGGTTTTAATTGTCTCTCTTGTCTACGATCAATATGAATTTGCTGCTGTGGCCTAACATTGTGCTCCTGATTGTTCTTTTCTCCCTTAATTTTACTCGATTGGAGACATTGAAGAGAAAATGTACACGTGAAATACCACGGCTTAAAATGATTTAATACATGTGGGCCTTTTTTATTCTTCCTCTGTAATGGTAAAAAGAAATCTAACTCTGTTTTACATGAACAGATAAGGCTGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGTTCCCAGCTAGTTGTATTTCCAGAAGCCTTTGTTGGTGGTTATCCTCGTGGATCAAATTTTGGTGTTTCCATTGGGAATAGAACTCCTAAGGGGAAAGAGGAATTTCGTAAATATCATGCCTCAGCGATTGATGTACCTGGTTAGTTTTTGTCTCCAGGTGCACTTTGTTTGACTCAATGTGAGTTGCTACTGTAAAGAGAAAAATCTTAGTGTCTTACCATGGATAATTTCAGGTTAAGTAAGTTTTGCTAGGCTCGTTGTATAATTCTCATTCTAGAGGGCTGACTTAATTTCTCCTTGAGTTTACCAAACTTGATTTCTGGTGATATATTAGTATTAAATTCTTTCATCGTAACATACACCTTCTGTTCTTTATCATCTTTCATTCCTAAATGGATGTTACCTCATATTAGGACAGTACAATAAGGAGCTACTGATGTCAGTCTATTCAAAGTGAAATACGAGTTTGTTGCTCATGAAATATGAAATGGGACTATGGTCTGTTTTAATGGCTCTTGCAGTTTGCAGTTAATGAATGACAAACAAGATTGCTTCAACTGCTGGCATTTCTTAGTTCTATTAATTTCAAAATCTTCCTACTCTTGCTTGCATCTAAAACCTAGACATAATATTGTAAAGTTCTGAAAGCTATAGCCAGTTCCTGTCTTAGTTGCTGTTTAGGTTTTTTTTTAAAACTCTATTTTGATAGGCTTCAGTTCATGTATGAGGTTGCTTTACTTCCATTCATCCTAATACAATCTGGTTGACCTTGTGTTTACTTCCATTCATCTAAGTAATATCTTGTTTTCTCATAAAAACCATTTTTATTCATCTATAAGATCATATGCCACATTCACAAAATAAACTGCTTGAAGTGCACCGGCTGTGGTTCTAATATATCAAATGATTGTTATTTTGATGACTATAGACGGTAATACATGCAGGTCCTGAAGTTGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTACATTTGGTAATGGGGGTGATAGAGAGAGATGGCTACACACTGTACTGTACTGTCCTATTTTTTGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAAGTCATGCCAACTGCATTGGAGCGAATAATCTGGGGCTTTGGTGATGGATCAACAATTCCTGTGTTTGAAACTTCAATTGGAAAGATAGGTGCTGCCATTTGTTGGGAAAACAGAATGCCACTCCTAAGAACAGCTATGTATGCAAAAGGTTAGTTATTCATTGCTGGCAATAATGTCATTCCTGACGTAATTGCGTATTTCAAATCTAGTTTTATTCTTTCTGTTTGACTGTATGATGTTTATTAAACTTAAAATAGATAGCATAGCACTACAAAAATGTGATGGGAATGGTATTAGAGGGATATTAAGGGTAAATTAGTAGTAAGTTAAAGAGTTTGTCAGGGAAGTTTGGTTATAAATATAATGAGTAAGGAAGAAAGGGTTTGACAATATTTTGGGAGTAAATTAGGGCTTTGAGTGACCTCAAGATTGGGAGGGTCCAAGTACCTCAAATTACATGGTCTATTTTGTATTTCTCTTATCTTTTATAGTTCAATATATTTGGGTTCTACCAAAAGACTGACGAGAAACTATTACAGACTAAAGAGGCTCTAGTTGTTAAGAAATTGTAAAACGATGGTCAAACTCCAGAGTTGGGGATTATTGTGTCACTGCTCACTATTTACAGACACTGGTCTATCATTTTTAGCTTCTTTTCTCAGAATATACTTGTTTGGTTAATCATATTGTGCACAACTTTTTTGTTTTATGTTTCTCTTTCAACTACATCAAGTTAAAAAGTACTTTACACCGAAACCAAATGGAAAAATCATATAGAAGAAGTTTTTCATATGCTTAAAGTTCTACTGCTATGTTAAGGATAGAAGTTGGTATTTCTTCAAACTTGCCTAACTTCCGTCTAAACAGTCCTTTTCTTTTGCTTAACCGAATTTGCTCACTAGTAATTGACTTATCATGTTTGTAGGCATTGAAATTTATTGTGCACCTACTGCTGATTCTCGGGATACATGGCAAGCATCAATGACCCACATTGCTTTGGAGGGTGGATGTTTTGTTTTGTCAGCCAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCAGAATATGTATTTTCTGGCACAGAAGAGGAACTGACCCCGGATTCTGTTGTTTGTGCTGGAGGTAGTGCGATCATATCACCTTCAGGGACAATCCTAGCTGGACCCAATTACGATGGAGAGGCACTTATTTCAGCAGATCTGGGTATGTCATCTCATCCTTCTGCTTATGGTCATCAGAGCTTTAGTATGTACTCCAACATTATCATTTTAAGTGGTTGATATAAGAAGGTTTTATCTTGAGGAACCTACTATTTTATTTGAGTCTCTTTCTTCTTCAAGTGAATACTAGGATTCTCTCTCCTGGATGATCTGATGCTCCTGACAGCCAACGATTCTGAAAACCTTTATTCCAAGCTCTTATTCTAAGCTTAGCCATTTTATTGTCAATATAAATGAACGGCCACTAGGATTTGGTTTCCAGTAAAATGGTTAAGGATCCATCAACCATTAAAGAGAGGAAGGGCCTCTCTTGCTGTCTAAAAAGTTCCTTGGAGAATGTGGGGATAAACCGAAGTGGTTTTTTTTAACCTTATGTGCCTTGAGTTATGAAACTTTTTCATCTAGATCTTTGTGAGCAAAGGTCGAACTAGGAATTCCTAAGCTAAGCCTCTTACCAAGGTAGAAGGGTTTTTGTGAAGGTTCTACTTGATAATATAAATAGAAAGCACAAAATTACATGGACAAGGCAATAACAACAATCTAACCACGAAAGGCCGAATAAAGGCCCAATCAAATCCCTTGAGGGAAAATTTTGATTTACCCCTTCTAACATGTCTGTGCACTATGTGGTCCTCACAGTTCGAATATTGAAGAATGTTAGATACCAAAGAGAGCTCCTAATATTTGACAGGCCAGGAAGACAGGAAGCAATAAAGCAAGAAAGAGTTATTGGCTTATCGCTATCGGATTTTTAATCTCCTATGACAGTCCCTCTTAGAAAAGCACAAATACAAAAAAGAACCCCTCCTAAGATCGGTCACTAATGCCAGCTACTTAACCATAAGAGATTGGTCTTCAAGCCAAACATATTCTTAAATGAATAAAGGATTGAAGACGGATACTCAAATCTCCCAATCATATGCCTGCAAATAAAGGTTAGAGAGATAAACCTGGTTCCAACTGATTTTTGGCACGGTTCAGTATGAATGATTATTGATCCATCCACACCAACTCTTAACAAATTAAACTTTTTGGAGCATATGTTTTATTGACTATTTACCCCTCCCTTCATTGATTGTTATGGAAAGCTTTTTTCTACAACTAGCATTTTTAGATGAAAAATAATTTCGATGAATATCTGAAACAGATCTTGGAGAAATAGCACGGGCAAAATTTGATTTCGATGTGGTTGGTCATTATGCAAGGCCTGAAGTTCTTAGCTTGGTTGTGAGGGATCATCCTACAACCCCGGTTACTTTCACATCTACATCTACAAAAGTCGAAGATTCTTGTAAAAAGTAGTACGTATTCTTCGTTCACACTTCTATTTAGTTCCATTAATTGTTTTAGCCAACATGCGTCCATGTTTTACTGTTTCCTCCGCGTTCAGTGTTGTATTATATTCATACTAAGAATTCAAAACTGTGAATATTTCTGTTAATTTTTATCAGAATTATATTTGCAGCAATTTGTCACGGTGGGTTTCTGTTCACATCATTTCCCCTCTATTTCTCTAACCTAGCTTGCTTATGATTCTGGATTTAGCTGTATCACTGATGCTAGTGTAAGAAATGTGGCCAATTAAGTTTTAGTATATTTGTGTTTGATACTTTGACAAGAGCATATAGGTTATTTTATTTTATTTGATACCTTCCTCAAAAAATCATATCATTCCTCTTCCTCTTCCTCTAATATCTCAATCCATCCAACACATCGTATTAAGCCACAAGAAACGGTCAAGTGACTTGTTTTATTTTATTTTGTAACCTTATTTATGTTAAAGTTCTGAAAAAAC

mRNA sequence

ATGGCGCTTGTGCCAACCACGGCCCCTACCGACTCCCAACTGATAGCGGAGGTTGACATGGGCTCCGATTCATCTGTAATGAATGTTCGAGCCACCGTCGTTCAGGCCTCCACCATCTTCTATGACACTCCGGCCACTCTAGATAAGGCTGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGTTCCCAGCTAGTTGTATTTCCAGAAGCCTTTGTTGGTGGTTATCCTCGTGGATCAAATTTTGGTGTTTCCATTGGGAATAGAACTCCTAAGGGGAAAGAGGAATTTCGTAAATATCATGCCTCAGCGATTGATGTACCTGGTCCTGAAGTTGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTACATTTGGTAATGGGGGTGATAGAGAGAGATGGCTACACACTGTACTGTACTGTCCTATTTTTTGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAAGTCATGCCAACTGCATTGGAGCGAATAATCTGGGGCTTTGGTGATGGATCAACAATTCCTGTGTTTGAAACTTCAATTGGAAAGATAGGTGCTGCCATTTGTTGGGAAAACAGAATGCCACTCCTAAGAACAGCTATGTATGCAAAAGGCATTGAAATTTATTGTGCACCTACTGCTGATTCTCGGGATACATGGCAAGCATCAATGACCCACATTGCTTTGGAGGGTGGATGTTTTGTTTTGTCAGCCAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCAGAATATGTATTTTCTGGCACAGAAGAGGAACTGACCCCGGATTCTGTTGTTTGTGCTGGAGGTAGTGCGATCATATCACCTTCAGGGACAATCCTAGCTGGACCCAATTACGATGGAGAGGCACTTATTTCAGCAGATCTGGATCTTGGAGAAATAGCACGGGCAAAATTTGATTTCGATGTGGTTGGTCATTATGCAAGGCCTGAAGTTCTTAGCTTGGTTGTGAGGGATCATCCTACAACCCCGGTTACTTTCACATCTACATCTACAAAAGTCGAAGATTCTTGTAAAAAGTAG

Coding sequence (CDS)

ATGGCGCTTGTGCCAACCACGGCCCCTACCGACTCCCAACTGATAGCGGAGGTTGACATGGGCTCCGATTCATCTGTAATGAATGTTCGAGCCACCGTCGTTCAGGCCTCCACCATCTTCTATGACACTCCGGCCACTCTAGATAAGGCTGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGTTCCCAGCTAGTTGTATTTCCAGAAGCCTTTGTTGGTGGTTATCCTCGTGGATCAAATTTTGGTGTTTCCATTGGGAATAGAACTCCTAAGGGGAAAGAGGAATTTCGTAAATATCATGCCTCAGCGATTGATGTACCTGGTCCTGAAGTTGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTACATTTGGTAATGGGGGTGATAGAGAGAGATGGCTACACACTGTACTGTACTGTCCTATTTTTTGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAAGTCATGCCAACTGCATTGGAGCGAATAATCTGGGGCTTTGGTGATGGATCAACAATTCCTGTGTTTGAAACTTCAATTGGAAAGATAGGTGCTGCCATTTGTTGGGAAAACAGAATGCCACTCCTAAGAACAGCTATGTATGCAAAAGGCATTGAAATTTATTGTGCACCTACTGCTGATTCTCGGGATACATGGCAAGCATCAATGACCCACATTGCTTTGGAGGGTGGATGTTTTGTTTTGTCAGCCAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCAGAATATGTATTTTCTGGCACAGAAGAGGAACTGACCCCGGATTCTGTTGTTTGTGCTGGAGGTAGTGCGATCATATCACCTTCAGGGACAATCCTAGCTGGACCCAATTACGATGGAGAGGCACTTATTTCAGCAGATCTGGATCTTGGAGAAATAGCACGGGCAAAATTTGATTTCGATGTGGTTGGTCATTATGCAAGGCCTGAAGTTCTTAGCTTGGTTGTGAGGGATCATCCTACAACCCCGGTTACTTTCACATCTACATCTACAAAAGTCGAAGATTCTTGTAAAAAGTAG

Protein sequence

MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK*
Homology
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BLAST of CsaV3_1G015110 vs. NCBI nr
Match: XP_004139500.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis sativus] >KGN64959.1 hypothetical protein Csa_022889 [Cucumis sativus])

HSP 1 Score: 713.8 bits (1841), Expect = 7.5e-202
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0 

Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 351
           LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 350

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BLAST of CsaV3_1G015110 vs. NCBI nr
Match: XP_008463648.1 (PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis melo] >KAA0035558.1 bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis melo var. makuwa] >TYK30976.1 bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis melo var. makuwa])

HSP 1 Score: 701.4 bits (1809), Expect = 3.8e-198
Identity = 343/350 (98.00%), Postives = 345/350 (98.57%), Query Frame = 0 

Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAPTD QLIAEVDMG+DSSVM VRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 351
           LGEIARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTSTSTK EDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 350

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BLAST of CsaV3_1G015110 vs. NCBI nr
Match: XP_023520715.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 692.2 bits (1785), Expect = 2.3e-195
Identity = 338/350 (96.57%), Postives = 343/350 (98.00%), Query Frame = 0 

Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA TD+QLIAEVDMGSD SV  VRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 351
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTS STK EDSCKK
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSASTKTEDSCKK 350

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BLAST of CsaV3_1G015110 vs. NCBI nr
Match: KAG6583706.1 (Bifunctional nitrilase/nitrile hydratase NIT4A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019353.1 Bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 689.9 bits (1779), Expect = 1.2e-194
Identity = 336/350 (96.00%), Postives = 343/350 (98.00%), Query Frame = 0 

Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA TD+QLIAEVDMGSD SV  VRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 351
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTS +TK EDSCKK
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK 350

[Close]
BLAST of CsaV3_1G015110 vs. NCBI nr
Match: XP_022973146.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita maxima])

HSP 1 Score: 686.0 bits (1769), Expect = 1.7e-193
Identity = 335/350 (95.71%), Postives = 340/350 (97.14%), Query Frame = 0 

Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA  D+QLIAEVDMGSD SV  VRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAANDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 351
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTS S K EDSC K
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSASKKTEDSCNK 350

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BLAST of CsaV3_1G015110 vs. ExPASy Swiss-Prot
Match: Q42965 (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Nicotiana tabacum OX=4097 GN=NIT4A PE=2 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 4.1e-179
Identity = 304/349 (87.11%), Postives = 323/349 (92.55%), Query Frame = 0 

Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPT A  +  L AEVDMG +SS   VRATVVQASTIFYDTPATL KAERLLAEAA +
Sbjct: 1   MALVPTPAVNEGPLFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLVKAERLLAEAASY 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           G+QLVVFPEAF+GGYPRGS FGVSIGNRT KGKEEFRKYHASAIDVPGPEVDRLAAMAGK
Sbjct: 61  GAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           Y+V+LVMGVIERDGYTLYCTVLFFD QG +LGKHRK+MPTALERIIWGFGDGSTIPV++T
Sbjct: 121 YKVYLVMGVIERDGYTLYCTVLFFDSQGHFLGKHRKIMPTALERIIWGFGDGSTIPVYDT 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
            +GKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRD WQASMTHIALEGGCFVLSAN
Sbjct: 181 PLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGTEE+LTPDS+VCAGGS IISPSG +LAGPNY GEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEDLTPDSIVCAGGSVIISPSGAVLAGPNYVGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCK 350
           LGEIARAKFDFDVVGHYARPEVLSL+VRDH  +PV+FTSTS+K E S K
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESSPK 349

[Close]
BLAST of CsaV3_1G015110 vs. ExPASy Swiss-Prot
Match: Q42966 (Bifunctional nitrilase/nitrile hydratase NIT4B OS=Nicotiana tabacum OX=4097 GN=NIT4B PE=2 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 1.6e-178
Identity = 303/349 (86.82%), Postives = 323/349 (92.55%), Query Frame = 0 

Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPT    +  + AEVDMG +SS   VRATVVQASTIFYDTPATLDKAERLLAEAA +
Sbjct: 1   MALVPTPVVNEGPMFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLDKAERLLAEAASY 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           G+QLVVFPEAF+GGYPRGS FGVSIGNRT KGKEEFRKYHASAIDVPGPEVDRLAAMAGK
Sbjct: 61  GAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           Y+V+LVMGVIERDGYTLYCTVLFFD QG YLGKHRK+MPTALERIIWGFGDGSTIPV++T
Sbjct: 121 YKVYLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKIMPTALERIIWGFGDGSTIPVYDT 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
            +GKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRD WQASMTHIALEGGCFVLSAN
Sbjct: 181 PLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGT E+LTPDS+VCAGGS IISPSG +LAGPNY+GEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGT-EDLTPDSIVCAGGSVIISPSGAVLAGPNYEGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCK 350
           LGEIARAKFDFDVVGHYARPEVLSL+VRDH  +PV+FTSTS+K E S K
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESSPK 348

[Close]
BLAST of CsaV3_1G015110 vs. ExPASy Swiss-Prot
Match: Q5QGZ8 (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Lupinus angustifolius OX=3871 GN=NIT4A PE=1 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 4.6e-178
Identity = 299/349 (85.67%), Postives = 322/349 (92.26%), Query Frame = 0 

Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALV T    D  L AEV+M SD +   VRATVVQASTIFYDTPATLDKAERLLAEAA +
Sbjct: 1   MALVTTPTVNDGPLFAEVNMSSDFNAPTVRATVVQASTIFYDTPATLDKAERLLAEAASY 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           G+Q+VVFPEAF+GGYPRGSNFGVSIGNRT KGKE+FRKYH++AIDVPGPEVDRLAA+AGK
Sbjct: 61  GAQIVVFPEAFIGGYPRGSNFGVSIGNRTAKGKEDFRKYHSAAIDVPGPEVDRLAALAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           Y+V+LVMGVIERDGYTLYCTVLFF  QGRYLGKHRK+MPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YKVYLVMGVIERDGYTLYCTVLFFGAQGRYLGKHRKLMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
            IGKIGAAICWEN+MPLLRTAMYAKG+EIYCAPTADSR+ WQASMTHIALEGGCFVLSAN
Sbjct: 181 PIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSREVWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRR+DYPPPPEYVF GTEE LTPDSVVCAGGS IISPSG +LAGP+Y+GEALISADLD
Sbjct: 241 QFCRRRDYPPPPEYVFEGTEENLTPDSVVCAGGSVIISPSGAVLAGPSYEGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCK 350
           LGEIARAKFDFDVVGHY+RPEVLSLVV+DHPT PVTFTS STK+ED  K
Sbjct: 301 LGEIARAKFDFDVVGHYSRPEVLSLVVKDHPTNPVTFTSASTKIEDKTK 349

[Close]
BLAST of CsaV3_1G015110 vs. ExPASy Swiss-Prot
Match: Q3LRV4 (Bifunctional nitrilase/nitrile hydratase NIT4B OS=Lupinus angustifolius OX=3871 GN=NIT4B PE=1 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 5.1e-177
Identity = 303/351 (86.32%), Postives = 324/351 (92.31%), Query Frame = 0 

Query: 1   MALVPTTAPT--DSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAA 60
           MALV TT PT  D  L AEVDM S  +   VRATVVQASTIFYDTPATLDKAERLL +AA
Sbjct: 1   MALV-TTTPTVNDEPLFAEVDMASYFTSTTVRATVVQASTIFYDTPATLDKAERLLVQAA 60

Query: 61  GFGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMA 120
            +G+Q+VVFPEAF+GGYPRGSNFGVSIGNRT KGKEEFRKYH++AIDVPGPEVDRL+AMA
Sbjct: 61  SYGAQIVVFPEAFIGGYPRGSNFGVSIGNRTAKGKEEFRKYHSAAIDVPGPEVDRLSAMA 120

Query: 121 GKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVF 180
           GKY+V+LVMGVIERDGYTLYCTVLFFD QGRYLGKHRKVMPTALERIIWGFGDGSTIPVF
Sbjct: 121 GKYKVYLVMGVIERDGYTLYCTVLFFDSQGRYLGKHRKVMPTALERIIWGFGDGSTIPVF 180

Query: 181 ETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLS 240
           +T IGKIGAAICWEN+MPLLRTAMYAKG+EIYCAPTADSRD WQAS THIALEGGCFVLS
Sbjct: 181 QTPIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSRDLWQASTTHIALEGGCFVLS 240

Query: 241 ANQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISAD 300
           ANQFCRRKDYPPPPEYVFSGTEE+LTPDSVV AGGS IISPSG +LAGPNY+GEALISAD
Sbjct: 241 ANQFCRRKDYPPPPEYVFSGTEEDLTPDSVVSAGGSVIISPSGAVLAGPNYEGEALISAD 300

Query: 301 LDLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCK 350
           LDLGEIARAKFDFDVVGHY+R EVLSL+V+DHPT PVTFTSTSTK+ED  K
Sbjct: 301 LDLGEIARAKFDFDVVGHYSRSEVLSLIVKDHPTNPVTFTSTSTKIEDQTK 350

[Close]
BLAST of CsaV3_1G015110 vs. ExPASy Swiss-Prot
Match: Q6H849 (Bifunctional nitrilase/nitrile hydratase NIT4 OS=Oryza sativa subsp. japonica OX=39947 GN=NIT4 PE=2 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 2.7e-162
Identity = 279/349 (79.94%), Postives = 307/349 (87.97%), Query Frame = 0 

Query: 1   MALVPTTAPTDSQLIAEVDM--GSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAA 60
           MA+VP+ +     +IAEV+M  G+DS    VRATVVQAST+FYDTPATLDKAERL+ EAA
Sbjct: 1   MAMVPSGSGGGPPVIAEVEMNGGADSGAATVRATVVQASTVFYDTPATLDKAERLIEEAA 60

Query: 61  GFGSQLVVFPEAFVGGYPRGSNFG----VSIGNRTPKGKEEFRKYHASAIDVPGPEVDRL 120
           G+GSQLVVFPEAFVGGYPRGS FG    +SIGN   KGKEEFRKYHA+AI+VPGPEV RL
Sbjct: 61  GYGSQLVVFPEAFVGGYPRGSTFGFGANISIGNPKDKGKEEFRKYHAAAIEVPGPEVTRL 120

Query: 121 AAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGST 180
           AAMAGKY+V LVMGVIER+GYTLYC+VLFFDP GRYLGKHRK+MPTALERIIWGFGDGST
Sbjct: 121 AAMAGKYKVFLVMGVIEREGYTLYCSVLFFDPLGRYLGKHRKLMPTALERIIWGFGDGST 180

Query: 181 IPVFETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGC 240
           IPV++T +GKIGA ICWEN+MPLLRTA+Y KGIEIYCAPTADSR  WQASMTHIALEGGC
Sbjct: 181 IPVYDTPLGKIGALICWENKMPLLRTALYGKGIEIYCAPTADSRQVWQASMTHIALEGGC 240

Query: 241 FVLSANQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEAL 300
           FVLSANQFCRRKDYPPPPEYVFSG  EE +PD+VVC GGS IISPSG +LAGPNY+GEAL
Sbjct: 241 FVLSANQFCRRKDYPPPPEYVFSGLGEEPSPDTVVCPGGSVIISPSGEVLAGPNYEGEAL 300

Query: 301 ISADLDLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTK 344
           I+ADLDLGEI RAKFDFDVVGHYARPEVLSLVV D P  PV+FTS + K
Sbjct: 301 ITADLDLGEIVRAKFDFDVVGHYARPEVLSLVVNDQPHLPVSFTSAAEK 349

[Close]
BLAST of CsaV3_1G015110 vs. ExPASy TrEMBL
Match: A0A0A0LVI0 (CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G168360 PE=3 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 3.6e-202
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0 

Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 351
           LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 350

[Close]
BLAST of CsaV3_1G015110 vs. ExPASy TrEMBL
Match: A0A5A7SY83 (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G002030 PE=3 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 1.9e-198
Identity = 343/350 (98.00%), Postives = 345/350 (98.57%), Query Frame = 0 

Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAPTD QLIAEVDMG+DSSVM VRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 351
           LGEIARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTSTSTK EDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 350

[Close]
BLAST of CsaV3_1G015110 vs. ExPASy TrEMBL
Match: A0A1S3CLB4 (bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo OX=3656 GN=LOC103501743 PE=3 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 1.9e-198
Identity = 343/350 (98.00%), Postives = 345/350 (98.57%), Query Frame = 0 

Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAPTD QLIAEVDMG+DSSVM VRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 351
           LGEIARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTSTSTK EDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 350

[Close]
BLAST of CsaV3_1G015110 vs. ExPASy TrEMBL
Match: A0A6J1I7W0 (bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita maxima OX=3661 GN=LOC111471660 PE=3 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 8.1e-194
Identity = 335/350 (95.71%), Postives = 340/350 (97.14%), Query Frame = 0 

Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA  D+QLIAEVDMGSD SV  VRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAANDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 351
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTS S K EDSC K
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSASKKTEDSCNK 350

[Close]
BLAST of CsaV3_1G015110 vs. ExPASy TrEMBL
Match: A0A6J1EHI6 (bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita moschata OX=3662 GN=LOC111434356 PE=3 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 1.4e-193
Identity = 334/349 (95.70%), Postives = 340/349 (97.42%), Query Frame = 0 

Query: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA  D+QLIAEVDMGSD SV  VRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAAADTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120
           GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCK 350
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTS + K EDSCK
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATAKTEDSCK 349

[Close]
BLAST of CsaV3_1G015110 vs. TAIR 10
Match: AT5G22300.1 (nitrilase 4 )

HSP 1 Score: 556.2 bits (1432), Expect = 1.9e-158
Identity = 271/343 (79.01%), Postives = 303/343 (88.34%), Query Frame = 0 

Query: 6   TTAPTDS--QLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQ 65
           T AP  +  Q+  E+DM +  S   VRATVVQAST+FYDTPATLDKAERLL+EAA  GSQ
Sbjct: 11  TAAPQTNGHQIFPEIDMSAGDSSSIVRATVVQASTVFYDTPATLDKAERLLSEAAENGSQ 70

Query: 66  LVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYRV 125
           LVVFPEAF+GGYPRGS F ++IG+RT KG+++FRKYHASAIDVPGPEV+RLA MA KY+V
Sbjct: 71  LVVFPEAFIGGYPRGSTFELAIGSRTAKGRDDFRKYHASAIDVPGPEVERLALMAKKYKV 130

Query: 126 HLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIG 185
           +LVMGVIER+GYTLYCTVLFFD QG +LGKHRK+MPTALER IWGFGDGSTIPVF+T IG
Sbjct: 131 YLVMGVIEREGYTLYCTVLFFDSQGLFLGKHRKLMPTALERCIWGFGDGSTIPVFDTPIG 190

Query: 186 KIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFC 245
           KIGAAICWENRMP LRTAMYAKGIEIYCAPTADSR+TW ASMTHIALEGGCFVLSANQFC
Sbjct: 191 KIGAAICWENRMPSLRTAMYAKGIEIYCAPTADSRETWLASMTHIALEGGCFVLSANQFC 250

Query: 246 RRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLGE 305
           RRKDYP PPEY+FSG+EE LTPDSVVCAGGS+IISP G +LAGPNY GEALI+ADLDLG+
Sbjct: 251 RRKDYPSPPEYMFSGSEESLTPDSVVCAGGSSIISPLGIVLAGPNYRGEALITADLDLGD 310

Query: 306 IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVED 347
           IARAKFDFDVVGHY+RPEV SL +R+HP   V+F  TS  +ED
Sbjct: 311 IARAKFDFDVVGHYSRPEVFSLNIREHPRKAVSF-KTSKVMED 352

[Close]
BLAST of CsaV3_1G015110 vs. TAIR 10
Match: AT3G44300.1 (nitrilase 2 )

HSP 1 Score: 469.5 bits (1207), Expect = 2.3e-132
Identity = 216/322 (67.08%), Postives = 261/322 (81.06%), Query Frame = 0 

Query: 29  VRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVSIGNR 88
           VRAT+VQAST++ DTPATL+KA + + EAA  GS+LVVFPEAF+GGYPRG  FG+ +G  
Sbjct: 18  VRATIVQASTVYNDTPATLEKANKFIVEAASKGSELVVFPEAFIGGYPRGFRFGLGVGVH 77

Query: 89  TPKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQG 148
             +G++EFRKYHASAI VPGPEV++LA +AGK  V+LVMG IE+DGYTLYCT LFF PQG
Sbjct: 78  NEEGRDEFRKYHASAIKVPGPEVEKLAELAGKNNVYLVMGAIEKDGYTLYCTALFFSPQG 137

Query: 149 RYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGIE 208
           ++LGKHRK+MPT+LER IWG GDGSTIPV++T IGK+GAAICWENRMPL RTA+YAKGIE
Sbjct: 138 QFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIE 197

Query: 209 IYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELTPDSV 268
           +YCAPTAD    WQ+SM HIA+EGGCFVLSA QFC RKD+P  P+Y+F+   ++  PDS+
Sbjct: 198 LYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCLRKDFPDHPDYLFTDWYDDKEPDSI 257

Query: 269 VCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIARAKFDFDVVGHYARPEVLSLVVR 328
           V  GGS IISP G +LAGPN++ E LI+ADLDLG++ARAK  FD VGHY+RP+VL L V 
Sbjct: 258 VSQGGSVIISPLGQVLAGPNFESEGLITADLDLGDVARAKLYFDSVGHYSRPDVLHLTVN 317

Query: 329 DHPTTPVTFTSTSTKVEDSCKK 351
           +HP  PVTF S   K ED   K
Sbjct: 318 EHPKKPVTFISKVEKAEDDSNK 339

[Close]
BLAST of CsaV3_1G015110 vs. TAIR 10
Match: AT3G44320.1 (nitrilase 3 )

HSP 1 Score: 464.9 bits (1195), Expect = 5.6e-131
Identity = 216/331 (65.26%), Postives = 264/331 (79.76%), Query Frame = 0 

Query: 20  MGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVVFPEAFVGGYPRGS 79
           +G D S   VR T+VQ+ST++ DTPATLDKAE+ + EAA  G++LV+FPEAF+GGYPRG 
Sbjct: 17  IGVDPS-STVRVTIVQSSTVYNDTPATLDKAEKFIVEAASKGAKLVLFPEAFIGGYPRGF 76

Query: 80  NFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYC 139
            FG+++G    +G++EFR YHASAI VPGPEV+RLA +AGK  VHLVMG IE+DGYTLYC
Sbjct: 77  RFGLAVGVHNEEGRDEFRNYHASAIKVPGPEVERLAELAGKNNVHLVMGAIEKDGYTLYC 136

Query: 140 TVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLR 199
           T LFF PQG++LGKHRKVMPT+LER IWG GDGSTIPV++T IGKIGAAICWENRMPL R
Sbjct: 137 TALFFSPQGQFLGKHRKVMPTSLERCIWGQGDGSTIPVYDTPIGKIGAAICWENRMPLYR 196

Query: 200 TAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGT 259
           TA+YAKGIEIYCAPTAD    WQASM HIA+EGGCFVLSA+QFC+R+++P  P+Y+F+  
Sbjct: 197 TALYAKGIEIYCAPTADYSLEWQASMIHIAVEGGCFVLSAHQFCKRREFPEHPDYLFNDI 256

Query: 260 EEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIARAKFDFDVVGHYAR 319
            +    D  V  GGS IISP G +LAGPNY+ E L++ADLDLG+IARAK  FDVVGHY++
Sbjct: 257 VDTKEHDPTVSGGGSVIISPLGKVLAGPNYESEGLVTADLDLGDIARAKLYFDVVGHYSK 316

Query: 320 PEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 351
           P++ +L V +HP  PVTF +   K ED   K
Sbjct: 317 PDIFNLTVNEHPKKPVTFMTKVEKAEDESNK 346

[Close]
BLAST of CsaV3_1G015110 vs. TAIR 10
Match: AT3G44310.1 (nitrilase 1 )

HSP 1 Score: 451.8 bits (1161), Expect = 4.9e-127
Identity = 207/322 (64.29%), Postives = 258/322 (80.12%), Query Frame = 0 

Query: 29  VRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVSIGNR 88
           VR T+VQ+ST++ DTPAT+DKAE+ + EAA  G++LV+FPE F+GGYPRG  FG+++G  
Sbjct: 25  VRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVGVH 84

Query: 89  TPKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQG 148
             +G++EFRKYHASAI VPGPEV RLA +A K  V+LVMG IE++GYTLYCTVLFF PQG
Sbjct: 85  NEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIEKEGYTLYCTVLFFSPQG 144

Query: 149 RYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGIE 208
           ++LGKHRK+MPT+LER IWG GDGSTIPV++T IGK+GAAICWENRMPL RTA+YAKGIE
Sbjct: 145 QFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIE 204

Query: 209 IYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELTPDSV 268
           +YCAPTAD    WQ+SM HIA+EGGCFVLSA QFC+RK +P  P+Y+F+   ++   DS+
Sbjct: 205 LYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCQRKHFPDHPDYLFTDWYDDKEHDSI 264

Query: 269 VCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIARAKFDFDVVGHYARPEVLSLVVR 328
           V  GGS IISP G +LAGPN++ E L++AD+DLG+IARAK  FD VGHY+RP+VL L V 
Sbjct: 265 VSQGGSVIISPLGQVLAGPNFESEGLVTADIDLGDIARAKLYFDSVGHYSRPDVLHLTVN 324

Query: 329 DHPTTPVTFTSTSTKVEDSCKK 351
           +HP   VTF +   K ED   K
Sbjct: 325 EHPRKSVTFVTKVEKAEDDSNK 346

[Close]
BLAST of CsaV3_1G015110 vs. TAIR 10
Match: AT3G44310.3 (nitrilase 1 )

HSP 1 Score: 451.8 bits (1161), Expect = 4.9e-127
Identity = 207/322 (64.29%), Postives = 258/322 (80.12%), Query Frame = 0 

Query: 29  VRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVSIGNR 88
           VR T+VQ+ST++ DTPAT+DKAE+ + EAA  G++LV+FPE F+GGYPRG  FG+++G  
Sbjct: 25  VRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVGVH 84

Query: 89  TPKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQG 148
             +G++EFRKYHASAI VPGPEV RLA +A K  V+LVMG IE++GYTLYCTVLFF PQG
Sbjct: 85  NEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIEKEGYTLYCTVLFFSPQG 144

Query: 149 RYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGIE 208
           ++LGKHRK+MPT+LER IWG GDGSTIPV++T IGK+GAAICWENRMPL RTA+YAKGIE
Sbjct: 145 QFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIE 204

Query: 209 IYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELTPDSV 268
           +YCAPTAD    WQ+SM HIA+EGGCFVLSA QFC+RK +P  P+Y+F+   ++   DS+
Sbjct: 205 LYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCQRKHFPDHPDYLFTDWYDDKEHDSI 264

Query: 269 VCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIARAKFDFDVVGHYARPEVLSLVVR 328
           V  GGS IISP G +LAGPN++ E L++AD+DLG+IARAK  FD VGHY+RP+VL L V 
Sbjct: 265 VSQGGSVIISPLGQVLAGPNFESEGLVTADIDLGDIARAKLYFDSVGHYSRPDVLHLTVN 324

Query: 329 DHPTTPVTFTSTSTKVEDSCKK 351
           +HP   VTF +   K ED   K
Sbjct: 325 EHPRKSVTFVTKVEKAEDDSNK 346

The following BLAST results are available for this feature:
BLAST of CsaV3_1G015110 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004139500.17.5e-202100.00bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis sativus] >KGN64959.1 hyp... [more]
XP_008463648.13.8e-19898.00PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis melo] >KAA003... [more]
XP_023520715.12.3e-19596.57bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita pepo subsp. pepo][more]
KAG6583706.11.2e-19496.00Bifunctional nitrilase/nitrile hydratase NIT4A, partial [Cucurbita argyrosperma ... [more]
XP_022973146.11.7e-19395.71bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita maxima][more]
BLAST of CsaV3_1G015110 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q429654.1e-17987.11Bifunctional nitrilase/nitrile hydratase NIT4A OS=Nicotiana tabacum OX=4097 GN=N... [more]
Q429661.6e-17886.82Bifunctional nitrilase/nitrile hydratase NIT4B OS=Nicotiana tabacum OX=4097 GN=N... [more]
Q5QGZ84.6e-17885.67Bifunctional nitrilase/nitrile hydratase NIT4A OS=Lupinus angustifolius OX=3871 ... [more]
Q3LRV45.1e-17786.32Bifunctional nitrilase/nitrile hydratase NIT4B OS=Lupinus angustifolius OX=3871 ... [more]
Q6H8492.7e-16279.94Bifunctional nitrilase/nitrile hydratase NIT4 OS=Oryza sativa subsp. japonica OX... [more]
BLAST of CsaV3_1G015110 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LVI03.6e-202100.00CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G16836... [more]
A0A5A7SY831.9e-19898.00Bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3CLB41.9e-19898.00bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1I7W08.1e-19495.71bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1EHI61.4e-19395.70bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita moschata OX=3662 GN=... [more]
BLAST of CsaV3_1G015110 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G22300.11.9e-15879.01nitrilase 4 [more]
AT3G44300.12.3e-13267.08nitrilase 2 [more]
AT3G44320.15.6e-13165.26nitrilase 3 [more]
AT3G44310.14.9e-12764.29nitrilase 1 [more]
AT3G44310.34.9e-12764.29nitrilase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036526Carbon-nitrogen hydrolase superfamilyGENE3D3.60.110.10coord: 19..313
e-value: 1.8E-86
score: 291.6
IPR036526Carbon-nitrogen hydrolase superfamilySUPERFAMILY56317Carbon-nitrogen hydrolasecoord: 29..327
IPR003010Carbon-nitrogen hydrolasePFAMPF00795CN_hydrolasecoord: 30..308
e-value: 1.1E-69
score: 234.7
IPR003010Carbon-nitrogen hydrolasePROSITEPS50263CN_HYDROLASEcoord: 29..301
score: 58.056446
NoneNo IPR availablePANTHERPTHR46044:SF1NITRILASEcoord: 13..343
IPR044149Nitrilase/Cyanide hydratasePANTHERPTHR46044NITRILASEcoord: 13..343
IPR044149Nitrilase/Cyanide hydrataseCDDcd07564nitrilases_CHscoord: 29..329
e-value: 4.91292E-163
score: 455.409
IPR000132Nitrilase/cyanide hydratase, conserved sitePROSITEPS00921NITRIL_CHT_2coord: 186..199
IPR000132Nitrilase/cyanide hydratase, conserved sitePROSITEPS00920NITRIL_CHT_1coord: 61..76

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_1G015110.1CsaV3_1G015110.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006807 nitrogen compound metabolic process
molecular_function GO:0003824 catalytic activity