CsaV3_1G005550 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_1G005550
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	Calcium-binding EF-hand family protein, putative
Location	chr1: 3614812 .. 3616314 (+)
RNA-Seq Expression	CsaV3_1G005550
Synteny	CsaV3_1G005550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CGAAGACAAAGCTTGACTTGGGATGTCACTGTTTGAACATAAAATTTCATTGCAAAATCCATCGAAACCCTTAGCCGACGGGCCGTTTGGAGAGAAGACTTGAAGAAGAAGATACCACGAGGCACGAAACTTCTTAGAACTTTATACAATTACTTCTTTAACACCAAACAACTATCTAAAGTCATTGCTTTCACTCCCTCCATTTCCCCATTTTCATCTTCTAATTTTCTCGGAAGTTACACTGTTGATTCTCAAAACACGCAAACCCAACCGCCCAATAATTCAGAAACAACAATCACTTTAATCCTTGACCCCAAATACAGACATTCTTTACTCCACATATAAATTGCCAACCCCATCAGTCTTCTTCTCTTATCTGCTTACTAAACTGCCCATTTACATATCTATGCTACTTGTTCTTTTACTCTTTTCAAAATGGAACCCATTGACGTCGTTGATTATACCAACCCCATTTCAGTTTTCCATATTTTGTTTCTGTTTTTTCTCAATTTCATACTTGGACGTTTCTTTCCAGGGTTATTCAACATTTTCATACTCTCTGCTGCATTTATTTTTTATCTCTGGAATTCATGGGTTGCCCAACGTAGCATCATCACACCAAGTGTTCGACGAAATGACCATGAAGAGCATTGCTGCTGCACAAAAATCGTGGGAATGCTTGTTAATGACAAACGGGTCGAGCAAGCGAAACTATCGATTGAAGATGTGAAAACTATGATGGAGGTACTTGATCATGAACATAATAACAATAATAGGGATAGTGATAGTGATAATCTTGAGCTTGAGGGGATGTTTGAGGAAGAAGTGAGTTTGGGGGAAGTTAAGGAAGCTTTTGATTTGTTTGATGAGAATGGTGATGGGTTTATTGATGCAGAAGATTTGAAGAAAGTGTTGTGTGGGTTGGGATTGATTACAGTGGGTTCAGACATTGATGAATGTAAGAGAATGTTAAGTGGGTTTGACAATGATGGAGATGGACGTTTAGATTTTGAAGAATTTGCTAAGCTTGTTGAACAGAGCTTCTGTTGAAAACATCTCTTTGTTTGTTAAAAACAGGACTCTTCCTAAATGTAAATTAGTCTTTGAAAATAAACAAACTGTTTTAGGTACTTGAAGGTTCATTTGTTACAAGACCATTTGTTTAAGTTTGTTTTTTTTGAAACGCTCTTCGTGAAAATGCACGAGGTATTTGTCGATGAACTAGAAGAAATATGTATGACAATATATAAGAAGAATTACTAATGCTCCGGTGGAGTTGATGATTATGTATTGTATTTTTTATTTAAGATTCAAATTGTGAAACCATTTCTATCTTTTTGGAAGAGACCCGTTGGAGTAAGCAAGCAGTGTGAAAGAGAAAGGTTTTGCTTTGAAAGACTTTCTTTCTAAAGCCACACTTTTAATTGAAGAAAAGAAAATGTTGAACTCTTTTCTTTTTGTATTCTTTTGAACAATACTTATGGATGCAAACTTAAAACTGTT

mRNA sequence

ATGGAACCCATTGACGTCGTTGATTATACCAACCCCATTTCAGTTTTCCATATTTTGTTTCTGTTTTTTCTCAATTTCATACTTGGACGTTTCTTTCCAGGGTTATTCAACATTTTCATACTCTCTGCTGCATTTATTTTTTATCTCTGGAATTCATGGGTTGCCCAACGTAGCATCATCACACCAAGTGTTCGACGAAATGACCATGAAGAGCATTGCTGCTGCACAAAAATCGTGGGAATGCTTGTTAATGACAAACGGGTCGAGCAAGCGAAACTATCGATTGAAGATGTGAAAACTATGATGGAGGTACTTGATCATGAACATAATAACAATAATAGGGATAGTGATAGTGATAATCTTGAGCTTGAGGGGATGTTTGAGGAAGAAGTGAGTTTGGGGGAAGTTAAGGAAGCTTTTGATTTGTTTGATGAGAATGGTGATGGGTTTATTGATGCAGAAGATTTGAAGAAAGTGTTGTGTGGGTTGGGATTGATTACAGTGGGTTCAGACATTGATGAATGTAAGAGAATGTTAAGTGGGTTTGACAATGATGGAGATGGACGTTTAGATTTTGAAGAATTTGCTAAGCTTGTTGAACAGAGCTTCTGTTGA

Coding sequence (CDS)

ATGGAACCCATTGACGTCGTTGATTATACCAACCCCATTTCAGTTTTCCATATTTTGTTTCTGTTTTTTCTCAATTTCATACTTGGACGTTTCTTTCCAGGGTTATTCAACATTTTCATACTCTCTGCTGCATTTATTTTTTATCTCTGGAATTCATGGGTTGCCCAACGTAGCATCATCACACCAAGTGTTCGACGAAATGACCATGAAGAGCATTGCTGCTGCACAAAAATCGTGGGAATGCTTGTTAATGACAAACGGGTCGAGCAAGCGAAACTATCGATTGAAGATGTGAAAACTATGATGGAGGTACTTGATCATGAACATAATAACAATAATAGGGATAGTGATAGTGATAATCTTGAGCTTGAGGGGATGTTTGAGGAAGAAGTGAGTTTGGGGGAAGTTAAGGAAGCTTTTGATTTGTTTGATGAGAATGGTGATGGGTTTATTGATGCAGAAGATTTGAAGAAAGTGTTGTGTGGGTTGGGATTGATTACAGTGGGTTCAGACATTGATGAATGTAAGAGAATGTTAAGTGGGTTTGACAATGATGGAGATGGACGTTTAGATTTTGAAGAATTTGCTAAGCTTGTTGAACAGAGCTTCTGTTGA

Protein sequence

MEPIDVVDYTNPISVFHILFLFFLNFILGRFFPGLFNIFILSAAFIFYLWNSWVAQRSIITPSVRRNDHEEHCCCTKIVGMLVNDKRVEQAKLSIEDVKTMMEVLDHEHNNNNRDSDSDNLELEGMFEEEVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITVGSDIDECKRMLSGFDNDGDGRLDFEEFAKLVEQSFC*

Homology

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BLAST of CsaV3_1G005550 vs. NCBI nr
Match: XP_004137586.1 (probable calcium-binding protein CML47 [Cucumis sativus] >KGN64007.1 hypothetical protein Csa_013235 [Cucumis sativus])

HSP 1 Score: 410.2 bits (1053), Expect = 1.0e-110
Identity = 204/204 (100.00%), Postives = 204/204 (100.00%), Query Frame = 0


Query: 1   MEPIDVVDYTNPISVFHILFLFFLNFILGRFFPGLFNIFILSAAFIFYLWNSWVAQRSII 60
           MEPIDVVDYTNPISVFHILFLFFLNFILGRFFPGLFNIFILSAAFIFYLWNSWVAQRSII
Sbjct: 1   MEPIDVVDYTNPISVFHILFLFFLNFILGRFFPGLFNIFILSAAFIFYLWNSWVAQRSII 60

Query: 61  TPSVRRNDHEEHCCCTKIVGMLVNDKRVEQAKLSIEDVKTMMEVLDHEHNNNNRDSDSDN 120
           TPSVRRNDHEEHCCCTKIVGMLVNDKRVEQAKLSIEDVKTMMEVLDHEHNNNNRDSDSDN
Sbjct: 61  TPSVRRNDHEEHCCCTKIVGMLVNDKRVEQAKLSIEDVKTMMEVLDHEHNNNNRDSDSDN 120

Query: 121 LELEGMFEEEVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITVGSDIDECKRMLS 180
           LELEGMFEEEVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITVGSDIDECKRMLS
Sbjct: 121 LELEGMFEEEVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITVGSDIDECKRMLS 180

Query: 181 GFDNDGDGRLDFEEFAKLVEQSFC 205
           GFDNDGDGRLDFEEFAKLVEQSFC
Sbjct: 181 GFDNDGDGRLDFEEFAKLVEQSFC 204

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BLAST of CsaV3_1G005550 vs. NCBI nr
Match: KAA0036089.1 (putative calcium-binding protein CML30 [Cucumis melo var. makuwa] >TYJ98897.1 putative calcium-binding protein CML30 [Cucumis melo var. makuwa])

HSP 1 Score: 310.8 bits (795), Expect = 8.5e-81
Identity = 169/216 (78.24%), Postives = 181/216 (83.80%), Query Frame = 0


Query: 1   MEPIDVVDYTNPISVFHILFLFFLNFILGRFFPGLFNIFILSAAFIFYLWNSWVAQRSII 60
           M P DV+DYTNPISVFHIL LFFLNFIL RFFPGLF IFIL AAF+FYLWNSW AQR+II
Sbjct: 1   MAPTDVIDYTNPISVFHILCLFFLNFILERFFPGLFYIFILYAAFLFYLWNSWAAQRNII 60

Query: 61  ----------TPSVRRNDHEEHCCCTKIVGMLVNDKRVEQAKLSIEDVKTMMEVLDHEHN 120
                     TPSVR+NDHEEH CCTKIVG  VNDKR  + KLSIEDVKTMME LDHEH 
Sbjct: 61  RRLEAADVTCTPSVRQNDHEEH-CCTKIVGTPVNDKR--EVKLSIEDVKTMMETLDHEH- 120

Query: 121 NNNRDSDSDNL--ELEGMFEEEVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITV 180
           NNNRDSD+  L  ELE MFEEE+SLGEV+EAFDLFDENGDGFIDAEDL+KVLCGLG   V
Sbjct: 121 NNNRDSDNLGLSAELEEMFEEELSLGEVQEAFDLFDENGDGFIDAEDLEKVLCGLG-FAV 180

Query: 181 GSDIDECKRMLSGFDNDGDGRLDFEEFAKLVEQSFC 205
            SDID+CK M+SGFDN+GDGRLDFEEFAKLVEQSFC
Sbjct: 181 ASDIDQCKTMISGFDNNGDGRLDFEEFAKLVEQSFC 211

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BLAST of CsaV3_1G005550 vs. NCBI nr
Match: XP_008445901.1 (PREDICTED: probable calcium-binding protein CML30 [Cucumis melo])

HSP 1 Score: 308.1 bits (788), Expect = 5.5e-80
Identity = 168/216 (77.78%), Postives = 180/216 (83.33%), Query Frame = 0


Query: 1   MEPIDVVDYTNPISVFHILFLFFLNFILGRFFPGLFNIFILSAAFIFYLWNSWVAQRSII 60
           M P DV+DYTNPISVFHIL LFFLNFIL RFFPGLF IFIL AAF+FYLWNSW AQR+II
Sbjct: 1   MAPTDVIDYTNPISVFHILCLFFLNFILERFFPGLFYIFILYAAFLFYLWNSWAAQRNII 60

Query: 61  ----------TPSVRRNDHEEHCCCTKIVGMLVNDKRVEQAKLSIEDVKTMMEVLDHEHN 120
                     TPSVR+NDHEEH CCTKIVG  VNDKR  + KLSIEDVKTMME LDHEH 
Sbjct: 61  RRLEAADVTCTPSVRQNDHEEH-CCTKIVGTPVNDKR--EVKLSIEDVKTMMETLDHEH- 120

Query: 121 NNNRDSDSDNL--ELEGMFEEEVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITV 180
           NNNRDSD+  L  ELE MFEEE+SLGEV+EAFDLFDEN DGFIDAEDL+KVLCGLG   V
Sbjct: 121 NNNRDSDNLGLSAELEEMFEEELSLGEVQEAFDLFDENDDGFIDAEDLEKVLCGLG-FAV 180

Query: 181 GSDIDECKRMLSGFDNDGDGRLDFEEFAKLVEQSFC 205
            SDID+CK M+SGFDN+GDGRLDFEEFAKLVEQSFC
Sbjct: 181 ASDIDQCKTMISGFDNNGDGRLDFEEFAKLVEQSFC 211

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BLAST of CsaV3_1G005550 vs. NCBI nr
Match: XP_038895011.1 (probable calcium-binding protein CML45 [Benincasa hispida])

HSP 1 Score: 262.7 bits (670), Expect = 2.7e-66
Identity = 141/213 (66.20%), Postives = 165/213 (77.46%), Query Frame = 0


Query: 1   MEPIDVVDYTNPISVFHILFLFFLNFILGRFFPGLFNIFILSAAFIFYLWNSWVAQRSII 60
           M PID +DY +PISVF ILFL FLNF+L RFFPGL  +FIL AAF+FYL NSW A R+II
Sbjct: 1   MAPIDELDYASPISVFQILFLIFLNFVLERFFPGLLYVFILCAAFLFYLCNSWAAHRNII 60

Query: 61  ----------TPSVRRNDHEEHCCCTKIVGMLVNDKRVEQAKLSIEDVKTMMEVLDHEHN 120
                     TPSVRRN HE H C T IV M VND   ++ KL+IEDVKTMME LDHEHN
Sbjct: 61  KRLEAADATCTPSVRRNAHEGH-CYTDIVVMPVND--TKEVKLTIEDVKTMMETLDHEHN 120

Query: 121 NNNRDSDSDNLELEGMFEEEVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITVGS 180
           N   ++   N E+ G+FEEE+SLGEVKEAFDLFD+NGDGFID EDLKKVLCG+G   V  
Sbjct: 121 NAESNNLELNAEIVGLFEEELSLGEVKEAFDLFDDNGDGFIDVEDLKKVLCGMG-FAVAL 180

Query: 181 DIDECKRMLSGFDNDGDGRLDFEEFAKLVEQSF 204
           ++D+C+RM+SGFD++GDGR+DFEEFAKLVEQSF
Sbjct: 181 EVDQCRRMISGFDSNGDGRIDFEEFAKLVEQSF 209

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BLAST of CsaV3_1G005550 vs. NCBI nr
Match: KAG7020054.1 (putative calcium-binding protein CML45, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 251.5 bits (641), Expect = 6.1e-63
Identity = 140/215 (65.12%), Postives = 160/215 (74.42%), Query Frame = 0


Query: 1   MEPIDVVDYTNPISVFHILFLFFLNFILGRFFPGLFNIFILSAAFIFYLWNSWVAQRSII 60
           M PID +DY NPISVF ILFLFFLNF+L   FPG   +F+L AAF FYLWNSWVA R+II
Sbjct: 1   MAPIDELDYANPISVFQILFLFFLNFLLEYLFPGPLYVFMLCAAFHFYLWNSWVAHRNII 60

Query: 61  -----------TPSVRRNDHEEHCCCTKIVGMLVNDKRVEQAKLSIEDVKTMMEVLDHEH 120
                      TPS RRN H  H C T IVGM VND    + KL+IEDVKTMME L   H
Sbjct: 61  KRFEAADGATCTPSARRNAHVGH-CYTGIVGMPVND--AHEVKLTIEDVKTMMEAL---H 120

Query: 121 NNNNRDSDSDNLELEGMFEEEVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITVG 180
           NN   ++     E+EG+FEEE+S+GEVKEAFDLFDENGDGFIDAEDLKKVL  +G  TV 
Sbjct: 121 NNMETNNLELKAEIEGVFEEELSVGEVKEAFDLFDENGDGFIDAEDLKKVLDAMG-FTVA 180

Query: 181 SDIDECKRMLSGFDNDGDGRLDFEEFAKLVEQSFC 205
           S++DEC+RM+SG+D +GDGRLDFEEFAKLVEQSFC
Sbjct: 181 SEVDECRRMISGYDKNGDGRLDFEEFAKLVEQSFC 208

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BLAST of CsaV3_1G005550 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 4.0e-17
Identity = 52/112 (46.43%), Postives = 70/112 (62.50%), Query Frame = 0


Query: 96  EDVKTMMEV--LDHEHNNNNRDSDSDNLELEGMFEE-EVSLGEVKEAFDLFDENGDGFID 155
           EDV  +M+   L  +  N        + E+  +FEE E SL EVK+AFD+FDEN DGFID
Sbjct: 94  EDVGMVMKSLGLSTDQENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDENRDGFID 153

Query: 156 AEDLKKVLCGLGLITVGSDIDECKRMLSGFDNDGDGRLDFEEFAKLVEQSFC 205
             DL++VL  LGL   GS+++ C+RM+  FD   DGR+DF  F K +E +FC
Sbjct: 154 PIDLQRVLTILGL-KQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFMENNFC 204

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BLAST of CsaV3_1G005550 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.2e-15
Identity = 50/121 (41.32%), Postives = 72/121 (59.50%), Query Frame = 0


Query: 84  NDKRVEQAKLSIEDVKTMMEVLDHEHNNNNRDSDSDNLELEGMFEE-EVSLGEVKEAFDL 143
           +D   +   +S E+ + +M  L   +N++         E+  +FEE E SL EVK+AFD+
Sbjct: 74  DDDDDDDIDISREEAEMVMRSLGLFYNDDQLQEQYSAKEVSSLFEEKEASLEEVKQAFDV 133

Query: 144 FDENGDGFIDAEDLKKVLCGLGLITVGSDIDECKRMLSGFDNDGDGRLDFEEFAKLVEQS 203
           FDEN DGFIDA +L++VL  LG    GS +D C  M+   D + DG++DF EF K +E S
Sbjct: 134 FDENKDGFIDAIELQRVLTILG-FKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETS 193

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BLAST of CsaV3_1G005550 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 6.1e-13
Identity = 35/75 (46.67%), Postives = 55/75 (73.33%), Query Frame = 0


Query: 126 MFEEEVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITVGSDIDECKRMLSGFDND 185
           M EE+    ++KEAF++FD+NGDGFI  ++LK VL  LGL   G  +D+CK+M+   D D
Sbjct: 70  MDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGL-KQGKTLDDCKKMIKKVDVD 129

Query: 186 GDGRLDFEEFAKLVE 201
           GDGR++++EF ++++
Sbjct: 130 GDGRVNYKEFRQMMK 143

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BLAST of CsaV3_1G005550 vs. ExPASy Swiss-Prot
Match: Q9SN89 (Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.0e-12
Identity = 67/194 (34.54%), Postives = 101/194 (52.06%), Query Frame = 0


Query: 11  NPISVFHI-LFLFFLNFILGRFFPGLFNIFILSAAFIFYLWNSWVAQRSIITPSVRRNDH 70
           +PIS+  I +FLF LN ++      +   F  S  F F+L+ S      I+  S+R N  
Sbjct: 8   SPISLLTIVIFLFILNLMM------IIQDFSSSFPFRFHLFFS---NAYILFTSIRNNKQ 67

Query: 71  EEHCCCTKIVGMLVNDKRVEQAKLSIEDVKTMMEVLDHEHNNNNRDSDSDNLELEGMFEE 130
                   I+  +V   R +  K S+E+VK +++  +  +     + +   LE   M  +
Sbjct: 68  NTE---LPIIKKVVVPNRAD-IKTSVEEVKAIIDDSEALYECLIEEGEEYLLEKNEMMGK 127

Query: 131 EVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITVGSDIDECKRMLSGFDNDGDGR 190
           E+    VKEAF LFDEN DGFID  +LK VL  LG         EC++M+  +D + DG+
Sbjct: 128 EI----VKEAFRLFDENQDGFIDENELKHVLSLLGYDECTK--MECRKMVKVYDENRDGK 182

Query: 191 LDFEEFAKLVEQSF 204
           +DF EF KL+E+SF
Sbjct: 188 IDFYEFVKLIEKSF 182

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BLAST of CsaV3_1G005550 vs. ExPASy Swiss-Prot
Match: Q9ZR02 (Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.3e-12
Identity = 34/74 (45.95%), Postives = 54/74 (72.97%), Query Frame = 0


Query: 128 EEEVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITVGSDIDECKRMLSGFDNDGD 187
           E+EV   ++KEAF++FD NGDGFI  ++LK VL  LGL   G  ++EC++M+   D DGD
Sbjct: 74  EDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGL-KQGKTLEECRKMIMQVDVDGD 133

Query: 188 GRLDFEEFAKLVEQ 202
           GR+++ EF +++++
Sbjct: 134 GRVNYMEFRQMMKK 146

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BLAST of CsaV3_1G005550 vs. ExPASy TrEMBL
Match: A0A0A0LTE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G033260 PE=4 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 5.0e-111
Identity = 204/204 (100.00%), Postives = 204/204 (100.00%), Query Frame = 0


Query: 1   MEPIDVVDYTNPISVFHILFLFFLNFILGRFFPGLFNIFILSAAFIFYLWNSWVAQRSII 60
           MEPIDVVDYTNPISVFHILFLFFLNFILGRFFPGLFNIFILSAAFIFYLWNSWVAQRSII
Sbjct: 1   MEPIDVVDYTNPISVFHILFLFFLNFILGRFFPGLFNIFILSAAFIFYLWNSWVAQRSII 60

Query: 61  TPSVRRNDHEEHCCCTKIVGMLVNDKRVEQAKLSIEDVKTMMEVLDHEHNNNNRDSDSDN 120
           TPSVRRNDHEEHCCCTKIVGMLVNDKRVEQAKLSIEDVKTMMEVLDHEHNNNNRDSDSDN
Sbjct: 61  TPSVRRNDHEEHCCCTKIVGMLVNDKRVEQAKLSIEDVKTMMEVLDHEHNNNNRDSDSDN 120

Query: 121 LELEGMFEEEVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITVGSDIDECKRMLS 180
           LELEGMFEEEVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITVGSDIDECKRMLS
Sbjct: 121 LELEGMFEEEVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITVGSDIDECKRMLS 180

Query: 181 GFDNDGDGRLDFEEFAKLVEQSFC 205
           GFDNDGDGRLDFEEFAKLVEQSFC
Sbjct: 181 GFDNDGDGRLDFEEFAKLVEQSFC 204

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BLAST of CsaV3_1G005550 vs. ExPASy TrEMBL
Match: A0A5A7T3X0 (Putative calcium-binding protein CML30 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00940 PE=4 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 4.1e-81
Identity = 169/216 (78.24%), Postives = 181/216 (83.80%), Query Frame = 0


Query: 1   MEPIDVVDYTNPISVFHILFLFFLNFILGRFFPGLFNIFILSAAFIFYLWNSWVAQRSII 60
           M P DV+DYTNPISVFHIL LFFLNFIL RFFPGLF IFIL AAF+FYLWNSW AQR+II
Sbjct: 1   MAPTDVIDYTNPISVFHILCLFFLNFILERFFPGLFYIFILYAAFLFYLWNSWAAQRNII 60

Query: 61  ----------TPSVRRNDHEEHCCCTKIVGMLVNDKRVEQAKLSIEDVKTMMEVLDHEHN 120
                     TPSVR+NDHEEH CCTKIVG  VNDKR  + KLSIEDVKTMME LDHEH 
Sbjct: 61  RRLEAADVTCTPSVRQNDHEEH-CCTKIVGTPVNDKR--EVKLSIEDVKTMMETLDHEH- 120

Query: 121 NNNRDSDSDNL--ELEGMFEEEVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITV 180
           NNNRDSD+  L  ELE MFEEE+SLGEV+EAFDLFDENGDGFIDAEDL+KVLCGLG   V
Sbjct: 121 NNNRDSDNLGLSAELEEMFEEELSLGEVQEAFDLFDENGDGFIDAEDLEKVLCGLG-FAV 180

Query: 181 GSDIDECKRMLSGFDNDGDGRLDFEEFAKLVEQSFC 205
            SDID+CK M+SGFDN+GDGRLDFEEFAKLVEQSFC
Sbjct: 181 ASDIDQCKTMISGFDNNGDGRLDFEEFAKLVEQSFC 211

[Close]

BLAST of CsaV3_1G005550 vs. ExPASy TrEMBL
Match: A0A1S3BEM1 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103488781 PE=4 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 2.7e-80
Identity = 168/216 (77.78%), Postives = 180/216 (83.33%), Query Frame = 0


Query: 1   MEPIDVVDYTNPISVFHILFLFFLNFILGRFFPGLFNIFILSAAFIFYLWNSWVAQRSII 60
           M P DV+DYTNPISVFHIL LFFLNFIL RFFPGLF IFIL AAF+FYLWNSW AQR+II
Sbjct: 1   MAPTDVIDYTNPISVFHILCLFFLNFILERFFPGLFYIFILYAAFLFYLWNSWAAQRNII 60

Query: 61  ----------TPSVRRNDHEEHCCCTKIVGMLVNDKRVEQAKLSIEDVKTMMEVLDHEHN 120
                     TPSVR+NDHEEH CCTKIVG  VNDKR  + KLSIEDVKTMME LDHEH 
Sbjct: 61  RRLEAADVTCTPSVRQNDHEEH-CCTKIVGTPVNDKR--EVKLSIEDVKTMMETLDHEH- 120

Query: 121 NNNRDSDSDNL--ELEGMFEEEVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITV 180
           NNNRDSD+  L  ELE MFEEE+SLGEV+EAFDLFDEN DGFIDAEDL+KVLCGLG   V
Sbjct: 121 NNNRDSDNLGLSAELEEMFEEELSLGEVQEAFDLFDENDDGFIDAEDLEKVLCGLG-FAV 180

Query: 181 GSDIDECKRMLSGFDNDGDGRLDFEEFAKLVEQSFC 205
            SDID+CK M+SGFDN+GDGRLDFEEFAKLVEQSFC
Sbjct: 181 ASDIDQCKTMISGFDNNGDGRLDFEEFAKLVEQSFC 211

[Close]

BLAST of CsaV3_1G005550 vs. ExPASy TrEMBL
Match: A0A6J1KID5 (probable calcium-binding protein CML47 OS=Cucurbita maxima OX=3661 GN=LOC111495509 PE=4 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 8.6e-63
Identity = 139/215 (64.65%), Postives = 160/215 (74.42%), Query Frame = 0


Query: 1   MEPIDVVDYTNPISVFHILFLFFLNFILGRFFPGLFNIFILSAAFIFYLWNSWVAQRSII 60
           M PID +DY NPISVF ILFLFFLNF+L   FPG   +FIL AAF FYLWNSWVA R+II
Sbjct: 12  MAPIDELDYANPISVFQILFLFFLNFLLEYLFPGPLYVFILCAAFHFYLWNSWVAHRNII 71

Query: 61  -----------TPSVRRNDHEEHCCCTKIVGMLVNDKRVEQAKLSIEDVKTMMEVLDHEH 120
                      TPS RRN H  H C T IVGM VND    + KL+IEDVKTMM+ L   H
Sbjct: 72  KRFEAADDATCTPSARRNAHMGH-CYTGIVGMPVND--AHEVKLTIEDVKTMMDAL---H 131

Query: 121 NNNNRDSDSDNLELEGMFEEEVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITVG 180
           NN   ++     E+EG+FEEE+S+GEV+EAFDLFDENGDGFIDAEDLKKVL  +G  TV 
Sbjct: 132 NNMETNNLELKAEIEGVFEEELSVGEVEEAFDLFDENGDGFIDAEDLKKVLDAMG-FTVA 191

Query: 181 SDIDECKRMLSGFDNDGDGRLDFEEFAKLVEQSFC 205
           S++DEC+RM+SG+D +GDGRLDFEEFAKLVEQSFC
Sbjct: 192 SEVDECRRMISGYDKNGDGRLDFEEFAKLVEQSFC 219

[Close]

BLAST of CsaV3_1G005550 vs. ExPASy TrEMBL
Match: A0A6J1ECX5 (probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC111431403 PE=4 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.9e-49
Identity = 114/176 (64.77%), Postives = 131/176 (74.43%), Query Frame = 0


Query: 40  ILSAAFIFYLWNSWVAQRSII-----------TPSVRRNDHEEHCCCTKIVGMLVNDKRV 99
           +L AAF FYLWNSWVA R+II           TPS RRN H  H C T IVGM VND   
Sbjct: 1   MLCAAFHFYLWNSWVAHRNIIKRFEASDGATCTPSARRNAHVGH-CYTGIVGMPVND--A 60

Query: 100 EQAKLSIEDVKTMMEVLDHEHNNNNRDSDSDNLELEGMFEEEVSLGEVKEAFDLFDENGD 159
            + KL+IEDVKTMME L   HNN   ++     E+EG+FEEE+S+GEVKEAFDLFDENGD
Sbjct: 61  HEVKLTIEDVKTMMEAL---HNNMETNNLELKAEIEGVFEEELSVGEVKEAFDLFDENGD 120

Query: 160 GFIDAEDLKKVLCGLGLITVGSDIDECKRMLSGFDNDGDGRLDFEEFAKLVEQSFC 205
           GFIDAEDLKKVL  +G  TV S++DEC+RM+SG+D +GDGRLDFEEFAKLVEQSFC
Sbjct: 121 GFIDAEDLKKVLDAMG-FTVASEVDECRRMISGYDKNGDGRLDFEEFAKLVEQSFC 169

[Close]

BLAST of CsaV3_1G005550 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 89.7 bits (221), Expect = 2.9e-18
Identity = 52/112 (46.43%), Postives = 70/112 (62.50%), Query Frame = 0


Query: 96  EDVKTMMEV--LDHEHNNNNRDSDSDNLELEGMFEE-EVSLGEVKEAFDLFDENGDGFID 155
           EDV  +M+   L  +  N        + E+  +FEE E SL EVK+AFD+FDEN DGFID
Sbjct: 94  EDVGMVMKSLGLSTDQENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDENRDGFID 153

Query: 156 AEDLKKVLCGLGLITVGSDIDECKRMLSGFDNDGDGRLDFEEFAKLVEQSFC 205
             DL++VL  LGL   GS+++ C+RM+  FD   DGR+DF  F K +E +FC
Sbjct: 154 PIDLQRVLTILGL-KQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFMENNFC 204

[Close]

BLAST of CsaV3_1G005550 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 84.0 bits (206), Expect = 1.6e-16
Identity = 50/121 (41.32%), Postives = 72/121 (59.50%), Query Frame = 0


Query: 84  NDKRVEQAKLSIEDVKTMMEVLDHEHNNNNRDSDSDNLELEGMFEE-EVSLGEVKEAFDL 143
           +D   +   +S E+ + +M  L   +N++         E+  +FEE E SL EVK+AFD+
Sbjct: 74  DDDDDDDIDISREEAEMVMRSLGLFYNDDQLQEQYSAKEVSSLFEEKEASLEEVKQAFDV 133

Query: 144 FDENGDGFIDAEDLKKVLCGLGLITVGSDIDECKRMLSGFDNDGDGRLDFEEFAKLVEQS 203
           FDEN DGFIDA +L++VL  LG    GS +D C  M+   D + DG++DF EF K +E S
Sbjct: 134 FDENKDGFIDAIELQRVLTILG-FKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETS 193

[Close]

BLAST of CsaV3_1G005550 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 75.9 bits (185), Expect = 4.3e-14
Identity = 35/75 (46.67%), Postives = 55/75 (73.33%), Query Frame = 0


Query: 126 MFEEEVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITVGSDIDECKRMLSGFDND 185
           M EE+    ++KEAF++FD+NGDGFI  ++LK VL  LGL   G  +D+CK+M+   D D
Sbjct: 70  MDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGL-KQGKTLDDCKKMIKKVDVD 129

Query: 186 GDGRLDFEEFAKLVE 201
           GDGR++++EF ++++
Sbjct: 130 GDGRVNYKEFRQMMK 143

[Close]

BLAST of CsaV3_1G005550 vs. TAIR 10
Match: AT3G47480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 75.1 bits (183), Expect = 7.3e-14
Identity = 67/194 (34.54%), Postives = 101/194 (52.06%), Query Frame = 0


Query: 11  NPISVFHI-LFLFFLNFILGRFFPGLFNIFILSAAFIFYLWNSWVAQRSIITPSVRRNDH 70
           +PIS+  I +FLF LN ++      +   F  S  F F+L+ S      I+  S+R N  
Sbjct: 8   SPISLLTIVIFLFILNLMM------IIQDFSSSFPFRFHLFFS---NAYILFTSIRNNKQ 67

Query: 71  EEHCCCTKIVGMLVNDKRVEQAKLSIEDVKTMMEVLDHEHNNNNRDSDSDNLELEGMFEE 130
                   I+  +V   R +  K S+E+VK +++  +  +     + +   LE   M  +
Sbjct: 68  NTE---LPIIKKVVVPNRAD-IKTSVEEVKAIIDDSEALYECLIEEGEEYLLEKNEMMGK 127

Query: 131 EVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITVGSDIDECKRMLSGFDNDGDGR 190
           E+    VKEAF LFDEN DGFID  +LK VL  LG         EC++M+  +D + DG+
Sbjct: 128 EI----VKEAFRLFDENQDGFIDENELKHVLSLLGYDECTK--MECRKMVKVYDENRDGK 182

Query: 191 LDFEEFAKLVEQSF 204
           +DF EF KL+E+SF
Sbjct: 188 IDFYEFVKLIEKSF 182

[Close]

BLAST of CsaV3_1G005550 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 73.9 bits (180), Expect = 1.6e-13
Identity = 34/74 (45.95%), Postives = 54/74 (72.97%), Query Frame = 0


Query: 128 EEEVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITVGSDIDECKRMLSGFDNDGD 187
           E+EV   ++KEAF++FD NGDGFI  ++LK VL  LGL   G  ++EC++M+   D DGD
Sbjct: 74  EDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGL-KQGKTLEECRKMIMQVDVDGD 133

Query: 188 GRLDFEEFAKLVEQ 202
           GR+++ EF +++++
Sbjct: 134 GRVNYMEFRQMMKK 146

The following BLAST results are available for this feature:

BLAST of CsaV3_1G005550 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137586.1	1.0e-110	100.00	probable calcium-binding protein CML47 [Cucumis sativus] >KGN64007.1 hypothetica...	[more]
KAA0036089.1	8.5e-81	78.24	putative calcium-binding protein CML30 [Cucumis melo var. makuwa] >TYJ98897.1 pu...	[more]
XP_008445901.1	5.5e-80	77.78	PREDICTED: probable calcium-binding protein CML30 [Cucumis melo]	[more]
XP_038895011.1	2.7e-66	66.20	probable calcium-binding protein CML45 [Benincasa hispida]	[more]
KAG7020054.1	6.1e-63	65.12	putative calcium-binding protein CML45, partial [Cucurbita argyrosperma subsp. a...	[more]

BLAST of CsaV3_1G005550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93Z27	4.0e-17	46.43	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q9MBG5	2.2e-15	41.32	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
Q9LNE7	6.1e-13	46.67	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
Q9SN89	1.0e-12	34.54	Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 ...	[more]
Q9ZR02	2.3e-12	45.95	Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1	[more]

BLAST of CsaV3_1G005550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LTE2	5.0e-111	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G033260 PE=4 SV=1	[more]
A0A5A7T3X0	4.1e-81	78.24	Putative calcium-binding protein CML30 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BEM1	2.7e-80	77.78	probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103488781 P...	[more]
A0A6J1KID5	8.6e-63	64.65	probable calcium-binding protein CML47 OS=Cucurbita maxima OX=3661 GN=LOC1114955...	[more]
A0A6J1ECX5	1.9e-49	64.77	probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]

BLAST of CsaV3_1G005550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G39670.1	2.9e-18	46.43	Calcium-binding EF-hand family protein	[more]
AT3G29000.1	1.6e-16	41.32	Calcium-binding EF-hand family protein	[more]
AT1G05990.1	4.3e-14	46.67	EF hand calcium-binding protein family	[more]
AT3G47480.1	7.3e-14	34.54	Calcium-binding EF-hand family protein	[more]
AT4G03290.1	1.6e-13	45.95	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 135..163 e-value: 4.0E-5 score: 33.0 coord: 174..202 e-value: 6.8E-4 score: 28.9
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 136..199 e-value: 3.3E-11 score: 43.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 170..204 score: 10.775421
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 131..166 score: 14.736568
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 135..199 e-value: 6.84482E-16 score: 67.1877
None	No IPR available	GENE3D	1.10.238.10		coord: 63..204 e-value: 7.8E-20 score: 73.5
None	No IPR available	PANTHER	PTHR10891:SF745	CALCIUM-BINDING PROTEIN CML46-RELATED	coord: 15..203
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 15..203
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 144..156
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 183..195
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 92..199

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_1G005550.1	CsaV3_1G005550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding