CsGy7G019270 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy7G019270
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationGy14Chr7: 21897622 .. 21900306 (+)
RNA-Seq ExpressionCsGy7G019270
SyntenyCsGy7G019270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTGACAAAAAAACACAACGAATCAGATCAATCATTCACAAATAGAGCCAAAATCAAACTCACATTTTTTTTATAAAAAAATTGTTCTCTCCTACATAACGAAAAAAATGGTAGCCGAGAGTTATTGAGGTCGTAAGAAACAAAATGGTTTGTGAAGCAAAATCCAAAGTGGTGATTCCCATAATAGATTTGTCAAAATTAGAGGAAGGCACAAAAGAATGGGGTTTGGCATGCAGAGAGATGAGATTTGGTTTAGAACAATTTGGTTGTTTTTCAGTGGTTTATGACAAAGTTTCTTTGGAGCTTCATAACTCCATTTTTGAAGCAACAAAAGAGCTTTTTCATCTCCCAATTGAAACGAGAATGAAGAACACAAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCATTTCTTCCTCTCTATGAATCCATGGCTATTGATAATCCAATTCAGCTACAACCAACTCAGAGTTTTGCCAATCTCATGTGGCCTACTACAGAAAACAATCACTTCTGGTAATTTCATTATTAACATAGATACGTACACACTATATATAGTACTTATTTTTGTTGTCCACCATCTGTATATATTTAGTAAATTCAGTTTGATTCTTATGATATTTGGAAGAGTCATTAAACTTCACCATATATGATTAATTCCTCGATGATTTATTTAGAATATTATATCACATATGTGTGATTGAAAATTTAAGACTTCTTTTTAAACATAACTAAAATGATCAAATTCACATGTCGGTTGTGCATAAAGATGATTTAATTGTTTAATTATTTGTTGATTTCTGAAGAAAAAAATTATTATTTTGGAAAATCACAGTGCCTAACAAATTGTGTTTATTTTCTTCTTAAACTTTAAAAAGTGTCTAATGAGTAATTAATTTTTTTTTCATATATAGGTCTGTCTAATATTTAATTCTTTTCCTTTTAATTTTATAATAATTTAGTCTCTAAATTTTGTAATTTTAATCTAACTTTTAAAACAATTCATCAAAATTAAAGGTTAATTTTTAGAAGTATAAGAATTTAGACTTTATAGCTTCTCACGTTAATAATTAGTTTATTGATCTGCCTAATGCAAGACATCTCACGTTAAAACTTAACTCTGATTTTCACTTAAATCGCAACAAATCTTTTAGAGAAACAAAAATTGTTACTTCAATGACCAAAAGTGATTTTGAACCAATTTTAATAATACCTCAAACTTTCAATTCGGTGTCTATGTTGATGACAGTGTAGCTGTTGAACGTTTCTCGACATTGGTGGCGAAATTGGAGCATATGGTAACAAAAATGGTGTTGGAAAGCTATGGAGTATCAAATAAGGTTGTGAGTGAACCAATAATGGAGTCCACAAATTACCTTCTTCGACTCTTCAAATACAGAGTAGCTGAGAAAGATGAAAACGATGTTGGTTTGCATTCCCATACTGATCTAACTTTCTTATCCATAATTCATCAGCATCTCATTTCTGGTCTTCAAATTCAATTACTTGATGATCAATGGCTCGAAGTTCACCCCTCATCTCATTGCTCCTTCACTGTCATGGCTGGCGATGCACTCATGGTAACTACACACCAATCCTTTCTTTTTGTTTTCTTTTCCCACAAAATTTAGGTCATGAACGTTAGTTATCGGATTAGAGAATATAATTATAAGTTTGTCTCGTGAAACCATATGATTGGAGGCCACAAGGTTGGATTTGCAAAAAAAATGAACTTTTAATTAAGAGGTTATACCCATATCTGTTATTTCTTAAAAACATGCATATATGAAAATATAAATTTAGTCTATCAACTTTATGATAGTTGTGTCAATCTTTTTATTTAATATCTAAGAGGTTAATATAGGTTTGGTTTTAACCATTCTATGAACTTTAAAAGTATATCTATTGTTGTCTTCTCTTATTGATCAACTCCACATGTTCAAGTTTCTGTTCTTTCATTTATGATTATTTTACTAACCCCAACTCCGTCATATATGTTCACATTTTTTCGTTACTCAAGCTTTATCGTCTCATCCATAAATTTAACAAGAGAAGCGGCCTTCATCAGTTACTTTCAAAGGAAAAAACTTTCAACTTTTCAAAGTTTGAGGAGTTATATAATTAACCATACATTACTTGATTGGTGGGTGTAGGCATGGAGCAACGAGAGAATAAGGTCTTGCAGGCATAGAGTGATAATGAGAGGAGAGGAAACAAGATATTCAATTGGGATGTTTTCATTCAAGAATGGGAGAATTGAAGTACCTCAAGAGTTTGTGGACGATGCCAACCCACTACGTTACAAGCCATTTCATCATTATGATTTCCTTACTTATGACAAAGCTAATGCTTCTCATAAAACTATCTCTCGTATCAAAGATTATTGTGGCCTTTGAATTCAACAAAGCGAAGCTTCCAGCTCATGCATGCACTCAATACTCAACAATCCTATATTTGATCTTTCACAATACTCCCTGAATATTTAGTATAATTATTTACTTATCTGTTAAGGTATGGTAAATGCTACGTTTTTCTTTCTTTCTTATGCAAAGTTTACACATATGGCTTCACATATGTAGTGTGGTTTTTCTAAATTGTTAAGGTATCATTTTGTAACAATTTGCTTTCATAGTCTAAACGTTGTTGCATATATATATAGTTGCTTCATCTTTTTTAAGGG

mRNA sequence

CTTGACAAAAAAACACAACGAATCAGATCAATCATTCACAAATAGAGCCAAAATCAAACTCACATTTTTTTTATAAAAAAATTGTTCTCTCCTACATAACGAAAAAAATGGTAGCCGAGAGTTATTGAGGTCGTAAGAAACAAAATGGTTTGTGAAGCAAAATCCAAAGTGGTGATTCCCATAATAGATTTGTCAAAATTAGAGGAAGGCACAAAAGAATGGGGTTTGGCATGCAGAGAGATGAGATTTGGTTTAGAACAATTTGGTTGTTTTTCAGTGGTTTATGACAAAGTTTCTTTGGAGCTTCATAACTCCATTTTTGAAGCAACAAAAGAGCTTTTTCATCTCCCAATTGAAACGAGAATGAAGAACACAAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCATTTCTTCCTCTCTATGAATCCATGGCTATTGATAATCCAATTCAGCTACAACCAACTCAGAGTTTTGCCAATCTCATGTGGCCTACTACAGAAAACAATCACTTCTGTGTAGCTGTTGAACGTTTCTCGACATTGGTGGCGAAATTGGAGCATATGGTAACAAAAATGGTGTTGGAAAGCTATGGAGTATCAAATAAGGTTGTGAGTGAACCAATAATGGAGTCCACAAATTACCTTCTTCGACTCTTCAAATACAGAGTAGCTGAGAAAGATGAAAACGATGTTGGTTTGCATTCCCATACTGATCTAACTTTCTTATCCATAATTCATCAGCATCTCATTTCTGGTCTTCAAATTCAATTACTTGATGATCAATGGCTCGAAGTTCACCCCTCATCTCATTGCTCCTTCACTGTCATGGCTGGCGATGCACTCATGGCATGGAGCAACGAGAGAATAAGGTCTTGCAGGCATAGAGTGATAATGAGAGGAGAGGAAACAAGATATTCAATTGGGATGTTTTCATTCAAGAATGGGAGAATTGAAGTACCTCAAGAGTTTGTGGACGATGCCAACCCACTACGTTACAAGCCATTTCATCATTATGATTTCCTTACTTATGACAAAGCTAATGCTTCTCATAAAACTATCTCTCGTATCAAAGATTATTGTGGCCTTTGAATTCAACAAAGCGAAGCTTCCAGCTCATGCATGCACTCAATACTCAACAATCCTATATTTGATCTTTCACAATACTCCCTGAATATTTAGTATAATTATTTACTTATCTGTTAAGGTATGGTAAATGCTACGTTTTTCTTTCTTTCTTATGCAAAGTTTACACATATGGCTTCACATATGTAGTGTGGTTTTTCTAAATTGTTAAGGTATCATTTTGTAACAATTTGCTTTCATAGTCTAAACGTTGTTGCATATATATATAGTTGCTTCATCTTTTTTAAGGG

Coding sequence (CDS)

ATGGTTTGTGAAGCAAAATCCAAAGTGGTGATTCCCATAATAGATTTGTCAAAATTAGAGGAAGGCACAAAAGAATGGGGTTTGGCATGCAGAGAGATGAGATTTGGTTTAGAACAATTTGGTTGTTTTTCAGTGGTTTATGACAAAGTTTCTTTGGAGCTTCATAACTCCATTTTTGAAGCAACAAAAGAGCTTTTTCATCTCCCAATTGAAACGAGAATGAAGAACACAAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCATTTCTTCCTCTCTATGAATCCATGGCTATTGATAATCCAATTCAGCTACAACCAACTCAGAGTTTTGCCAATCTCATGTGGCCTACTACAGAAAACAATCACTTCTGTGTAGCTGTTGAACGTTTCTCGACATTGGTGGCGAAATTGGAGCATATGGTAACAAAAATGGTGTTGGAAAGCTATGGAGTATCAAATAAGGTTGTGAGTGAACCAATAATGGAGTCCACAAATTACCTTCTTCGACTCTTCAAATACAGAGTAGCTGAGAAAGATGAAAACGATGTTGGTTTGCATTCCCATACTGATCTAACTTTCTTATCCATAATTCATCAGCATCTCATTTCTGGTCTTCAAATTCAATTACTTGATGATCAATGGCTCGAAGTTCACCCCTCATCTCATTGCTCCTTCACTGTCATGGCTGGCGATGCACTCATGGCATGGAGCAACGAGAGAATAAGGTCTTGCAGGCATAGAGTGATAATGAGAGGAGAGGAAACAAGATATTCAATTGGGATGTTTTCATTCAAGAATGGGAGAATTGAAGTACCTCAAGAGTTTGTGGACGATGCCAACCCACTACGTTACAAGCCATTTCATCATTATGATTTCCTTACTTATGACAAAGCTAATGCTTCTCATAAAACTATCTCTCGTATCAAAGATTATTGTGGCCTTTGA

Protein sequence

MVCEAKSKVVIPIIDLSKLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTTENNHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKDENDVGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSNERIRSCRHRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKANASHKTISRIKDYCGL*
Homology
BLAST of CsGy7G019270 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.2e-57
Identity = 120/301 (39.87%), Postives = 180/301 (59.80%), Query Frame = 0

Query: 11  IPIIDLS--KLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEATKELFHL 70
           +P+ID S   L+ G+ +W     ++   LE +GCF   +DK+S+EL+ S+FEA ++LF L
Sbjct: 14  LPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLFEL 73

Query: 71  PIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTTENNHFCVA 130
           PI T+ +N S KP+HGY        LYES+ ID+   L+    F   +WP   N      
Sbjct: 74  PIPTKQRNVSSKPFHGYL----CHNLYESLGIDDANVLEKVNDFTQQLWPDHGNKSISET 133

Query: 131 VERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKDEND---- 190
           +  FS  + +L+ MV +M++ES+G+ N +  +  + ST YL RL KY     D++D    
Sbjct: 134 IHLFSEQLVELDLMVRRMIMESFGIENYI--DEHLNSTYYLTRLMKYTSPPDDDDDDEET 193

Query: 191 -VGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSNERI 250
            +GL SHTD   ++I+HQ+ + GL+++  DD+W++V PS   S  VM GD+L A  N R+
Sbjct: 194 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQD-SVLVMVGDSLCALLNGRL 253

Query: 251 RSCRHRVIMRGEETRYSIGMFSF-KNG-RIEVPQEFVDDANPLRYKPFHHYDFLTYDKAN 303
            S  HRVIM G++TRYS G+FS  K G  I+ P+E VD  +P  +KPF + DFL + +  
Sbjct: 254 HSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTE 307

BLAST of CsGy7G019270 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 4.7e-57
Identity = 119/302 (39.40%), Postives = 180/302 (59.60%), Query Frame = 0

Query: 11  IPIIDLS--KLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEATKELFHL 70
           +P+ID S   L+ G+ +W     ++   LE +GCF   +DK+S+EL+ S+FEA ++LF L
Sbjct: 14  LPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFEL 73

Query: 71  PIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTTENNHFCVA 130
           PI T+ +N S KP+HGY        LYES+ I++   L+    F   +WP   N      
Sbjct: 74  PIPTKQRNVSSKPFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSISET 133

Query: 131 VERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKDEND---- 190
           +  FS  + +L+ MV +M++ES+G+ N +  +  + ST YL RL KY     D++D    
Sbjct: 134 IHLFSEQLVELDLMVRRMIMESFGIENYI--DEHLNSTYYLTRLMKYTSPPDDDDDDDEE 193

Query: 191 --VGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSNER 250
             +GL SHTD   ++I+HQ+ + GL+++  DD+W++V PS   S  VM GD+L A  N R
Sbjct: 194 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQD-SVLVMVGDSLCALLNGR 253

Query: 251 IRSCRHRVIMRGEETRYSIGMFSF-KNG-RIEVPQEFVDDANPLRYKPFHHYDFLTYDKA 303
           + S  HRVIM G++TRYS G+FS  K G  I+ P+E VD  +P  +KPF + DFL + + 
Sbjct: 254 LHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQT 308

BLAST of CsGy7G019270 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 2.0e-55
Identity = 117/300 (39.00%), Postives = 178/300 (59.33%), Query Frame = 0

Query: 11  IPIIDLS--KLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEATKELFHL 70
           +P+ID S   L+ G+ +W     ++   LE +GCF   +DK+S+EL+ S+FEA ++LF L
Sbjct: 7   LPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFEL 66

Query: 71  PIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTTENNHFCVA 130
           PI T+ +N S K +HGY        LYES+ I++   L+    F   +WP   N      
Sbjct: 67  PIPTKQRNVSSKLFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSISET 126

Query: 131 VERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKDEND---- 190
           +  FS  + +L+ MV +M++ES+G+   +  +  + ST YL RL KY     D++D    
Sbjct: 127 IHLFSEQLVELDLMVRRMIMESFGIEKYI--DEHLNSTYYLTRLMKYTSPPDDDDDEETK 186

Query: 191 VGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSNERIR 250
           +GL SHTD   ++I+HQ+ + GL+++  DD+W++V PS   S  VM GD+L A  N R+ 
Sbjct: 187 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQD-SVLVMVGDSLCALLNGRLH 246

Query: 251 SCRHRVIMRGEETRYSIGMFSF-KNG-RIEVPQEFVDDANPLRYKPFHHYDFLTYDKANA 303
           S  HRVIM G++TRYS G+FS  K G  I+ P+E VD  +P  +KPF + DFL + +  A
Sbjct: 247 SPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTEA 299

BLAST of CsGy7G019270 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.1e-48
Identity = 107/305 (35.08%), Postives = 158/305 (51.80%), Query Frame = 0

Query: 11  IPIIDLS--KLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEATKELFHL 70
           +P+IDLS   L+ GT  W     ++R  LE++GCF  V D VS EL  ++     EL +L
Sbjct: 9   LPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCNKGHELLNL 68

Query: 71  PIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTTENNHFCVA 130
           P+ET+M N ++  Y G+    S   L E M +     L+  + F NLMWP   N  FC  
Sbjct: 69  PLETKMLNGNKPEYDGF---TSIPNLNEGMGVGRITDLEKVERFTNLMWP-EGNKDFCET 128

Query: 131 VERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKDENDVGLH 190
           V  +   +A+++H++  MV ES+G+      +   ESTNYLL   +Y+   KD     L 
Sbjct: 129 VYSYGKRMAEVDHILKMMVFESFGMEKHF--DSFCESTNYLLHFMRYQQPGKDGRSPALS 188

Query: 191 SHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSNERIRSCRH 250
            H D + L+I++Q+ + GL+ +  D +W+     +H    V+ GD  MAWSN R+ S  H
Sbjct: 189 LHKDKSILTIVNQNDVKGLEFETKDGEWILPTADNH---IVLLGDCFMAWSNGRLHSPLH 248

Query: 251 RVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKANASHKTIS 310
           RV +   + R S   FSF    IE P E VD+ +PL + PF   + L Y       K + 
Sbjct: 249 RVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKEGAKAVC 304

Query: 311 RIKDY 314
            +K Y
Sbjct: 309 DLKQY 304

BLAST of CsGy7G019270 vs. ExPASy Swiss-Prot
Match: Q9ZTA2 (Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 4.9e-38
Identity = 109/393 (27.74%), Postives = 176/393 (44.78%), Query Frame = 0

Query: 11  IPIIDLS--KLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEATKELFHL 70
           +P+I+L+   LE G+ +W     ++R  LE FGCF   YDKVSLEL  SI +  +ELF L
Sbjct: 8   LPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELFAL 67

Query: 71  PIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTTE-NNHFCV 130
           P+ET+ +N   KPY GY    +   L ES+ I N   L+    F   +WP  + N +   
Sbjct: 68  PVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENINEFTQQLWPHGDGNENISK 127

Query: 131 AVERFSTLVAKLEHMVTKMVLESYGVSNKV--------------------------VSEP 190
            ++ F+  + +++ MV +MV+ES+G+   +                          V+  
Sbjct: 128 TIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKSTAYATDELNIVGVEPNVGVKVNAD 187

Query: 191 IMESTNYLLRL-----------------------------------------FKYRVA-- 250
           I +  N    +                                             VA  
Sbjct: 188 ISDDVNANASVNAGVGANVNADTGVNDNLNVDANVAVGGGVNANTDLGVGVNVNSNVAVN 247

Query: 251 ------------EKDENDVGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCS 310
                       + +E  +GL  HTD    +++ QH I GL+++  D++W+ V PS + +
Sbjct: 248 AKTGGDDVEANDDNEEKKLGLPCHTDKNLFTVLFQHEIEGLEVKTKDEKWIRVKPSPN-T 307

Query: 311 FTVMAGDALMAWSNERIRSCRHRV-IMRGEETRYSIGMFSFKNGR--IEVPQEFVDDANP 317
           F V+AGD+L A  N RIR+  HRV +   + TRY+  +F+       IE P+E VD+ +P
Sbjct: 308 FIVIAGDSLCALMNGRIRAPYHRVRVTEKKRTRYTAAIFTCPKPDYVIEAPKELVDEKHP 367

BLAST of CsGy7G019270 vs. NCBI nr
Match: XP_004136073.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis sativus])

HSP 1 Score: 652 bits (1682), Expect = 2.05e-236
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MVCEAKSKVVIPIIDLSKLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFE 60
           MVCEAKSKVVIPIIDLSKLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFE
Sbjct: 1   MVCEAKSKVVIPIIDLSKLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFE 60

Query: 61  ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTT 120
           ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTT
Sbjct: 61  ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTT 120

Query: 121 ENNHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEK 180
           ENNHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEK
Sbjct: 121 ENNHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEK 180

Query: 181 DENDVGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSN 240
           DENDVGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSN
Sbjct: 181 DENDVGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSN 240

Query: 241 ERIRSCRHRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKA 300
           ERIRSCRHRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKA
Sbjct: 241 ERIRSCRHRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKA 300

Query: 301 NASHKTISRIKDYCGL 316
           NASHKTISRIKDYCGL
Sbjct: 301 NASHKTISRIKDYCGL 316

BLAST of CsGy7G019270 vs. NCBI nr
Match: XP_008461057.1 (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo])

HSP 1 Score: 597 bits (1540), Expect = 9.45e-215
Identity = 294/317 (92.74%), Postives = 301/317 (94.95%), Query Frame = 0

Query: 1   MVCEAKSKVVIPIIDLSKLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFE 60
           MVCEAKSKVVIP IDLSKLE GTKEWGLAC+EMRFGLE+FGCFSVVYDKVSLELHNSI E
Sbjct: 1   MVCEAKSKVVIPTIDLSKLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVE 60

Query: 61  ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTT 120
           ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQL+ TQSFANLMWPT 
Sbjct: 61  ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTR 120

Query: 121 ENNHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEK 180
           +NNHFCV VERFSTLVAKLE MVTKMVLESYGVS KVV+EPIMESTNYLLRLFKYRVAEK
Sbjct: 121 DNNHFCVTVERFSTLVAKLEKMVTKMVLESYGVS-KVVNEPIMESTNYLLRLFKYRVAEK 180

Query: 181 DENDVGLHSHTDLTFLSIIHQHLISGLQIQLL-DDQWLEVHPSSHCSFTVMAGDALMAWS 240
           DE DVGLHSHTDLTF SIIHQHLISGLQIQ L DDQWL+VHPSSHCSF VMAGDALMAWS
Sbjct: 181 DETDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWS 240

Query: 241 NERIRSCRHRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDK 300
           NERIRSCRHRVIMRGEETRYSIGMFSFKNG IEVPQ FVDDANPLRYKPF+HYDFLTYDK
Sbjct: 241 NERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDK 300

Query: 301 ANASHKTISRIKDYCGL 316
           ANASH TISRIKDYCGL
Sbjct: 301 ANASHNTISRIKDYCGL 316

BLAST of CsGy7G019270 vs. NCBI nr
Match: XP_031744410.1 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X2 [Cucumis sativus] >KAE8646456.1 hypothetical protein Csa_016394 [Cucumis sativus])

HSP 1 Score: 541 bits (1395), Expect = 2.16e-193
Identity = 271/316 (85.76%), Postives = 271/316 (85.76%), Query Frame = 0

Query: 1   MVCEAKSKVVIPIIDLSKLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFE 60
           MVCEAKSKVVIPIIDLSKLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFE
Sbjct: 1   MVCEAKSKVVIPIIDLSKLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFE 60

Query: 61  ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTT 120
           ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTT
Sbjct: 61  ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTT 120

Query: 121 ENNHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEK 180
           ENNHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNK                        
Sbjct: 121 ENNHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNK------------------------ 180

Query: 181 DENDVGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSN 240
                                HLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSN
Sbjct: 181 ---------------------HLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSN 240

Query: 241 ERIRSCRHRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKA 300
           ERIRSCRHRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKA
Sbjct: 241 ERIRSCRHRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKA 271

Query: 301 NASHKTISRIKDYCGL 316
           NASHKTISRIKDYCGL
Sbjct: 301 NASHKTISRIKDYCGL 271

BLAST of CsGy7G019270 vs. NCBI nr
Match: XP_038898684.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 543 bits (1398), Expect = 3.96e-193
Identity = 262/314 (83.44%), Postives = 284/314 (90.45%), Query Frame = 0

Query: 4   EAKSKVVIPIIDLSKLEEG-TKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEAT 63
           EAK+KV +P IDLSKLE G T+EWGLAC+EMR GLE+FGCFSVVYD+VSLELHNSIFEAT
Sbjct: 5   EAKAKV-LPTIDLSKLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEAT 64

Query: 64  KELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTTEN 123
           KELFHLP+ETR+KNTSEKPYHGYFGGYS LPLYESM IDNPI+LQ TQSFANLMWPT EN
Sbjct: 65  KELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKEN 124

Query: 124 NHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKDE 183
           NHFC  VERFSTLVA+LE MVTKMVLESYG+S K+V+EP MES NYLLR FKYR  EKDE
Sbjct: 125 NHFCGTVERFSTLVAELEKMVTKMVLESYGLS-KLVNEPNMESANYLLRCFKYRAPEKDE 184

Query: 184 NDVGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSNER 243
            DVGLHSHTDLTF SIIHQHLISGLQ+Q LDDQW+++ PS HC F VMAGDALMAWSNER
Sbjct: 185 TDVGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNER 244

Query: 244 IRSCRHRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKANA 303
           IRSCRHRVIMRGEETRYSIGMFSFKNG I VP+E VDDANPLRYKPF+HYDFLTYDKA A
Sbjct: 245 IRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKAKA 304

Query: 304 SHKTISRIKDYCGL 316
           SH+ +SRIKDYCG+
Sbjct: 305 SHQNVSRIKDYCGI 316

BLAST of CsGy7G019270 vs. NCBI nr
Match: KAA0058792.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo var. makuwa] >TYK10586.1 putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 459 bits (1182), Expect = 2.28e-161
Identity = 225/244 (92.21%), Postives = 231/244 (94.67%), Query Frame = 0

Query: 74  MKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTTENNHFCVAVERFS 133
           MKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQL+ TQSFANLMWPT +NNHFCV VERFS
Sbjct: 1   MKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCVTVERFS 60

Query: 134 TLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKDENDVGLHSHTDL 193
           TLVAKLE MVTKMVLESYGVS KVV+EPIMESTNYLLRLFKYRVAEKDE DVGLHSHTDL
Sbjct: 61  TLVAKLEKMVTKMVLESYGVS-KVVNEPIMESTNYLLRLFKYRVAEKDETDVGLHSHTDL 120

Query: 194 TFLSIIHQHLISGLQIQLL-DDQWLEVHPSSHCSFTVMAGDALMAWSNERIRSCRHRVIM 253
           TF SIIHQHLISGLQIQ L DDQWL+VHPSSHCSF VMAGDALMAWSNERIRSCRH+VIM
Sbjct: 121 TFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCRHKVIM 180

Query: 254 RGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKANASHKTISRIKD 313
           RGEETRYSIGMFSFKNG IEVPQ FVDDANPLRYKPF+HYDFLTYDKANASH TISRIKD
Sbjct: 181 RGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTISRIKD 240

Query: 314 YCGL 316
           YCGL
Sbjct: 241 YCGL 243

BLAST of CsGy7G019270 vs. ExPASy TrEMBL
Match: A0A1S3CDV2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499759 PE=3 SV=1)

HSP 1 Score: 597 bits (1540), Expect = 4.58e-215
Identity = 294/317 (92.74%), Postives = 301/317 (94.95%), Query Frame = 0

Query: 1   MVCEAKSKVVIPIIDLSKLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFE 60
           MVCEAKSKVVIP IDLSKLE GTKEWGLAC+EMRFGLE+FGCFSVVYDKVSLELHNSI E
Sbjct: 1   MVCEAKSKVVIPTIDLSKLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVE 60

Query: 61  ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTT 120
           ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQL+ TQSFANLMWPT 
Sbjct: 61  ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTR 120

Query: 121 ENNHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEK 180
           +NNHFCV VERFSTLVAKLE MVTKMVLESYGVS KVV+EPIMESTNYLLRLFKYRVAEK
Sbjct: 121 DNNHFCVTVERFSTLVAKLEKMVTKMVLESYGVS-KVVNEPIMESTNYLLRLFKYRVAEK 180

Query: 181 DENDVGLHSHTDLTFLSIIHQHLISGLQIQLL-DDQWLEVHPSSHCSFTVMAGDALMAWS 240
           DE DVGLHSHTDLTF SIIHQHLISGLQIQ L DDQWL+VHPSSHCSF VMAGDALMAWS
Sbjct: 181 DETDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWS 240

Query: 241 NERIRSCRHRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDK 300
           NERIRSCRHRVIMRGEETRYSIGMFSFKNG IEVPQ FVDDANPLRYKPF+HYDFLTYDK
Sbjct: 241 NERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDK 300

Query: 301 ANASHKTISRIKDYCGL 316
           ANASH TISRIKDYCGL
Sbjct: 301 ANASHNTISRIKDYCGL 316

BLAST of CsGy7G019270 vs. ExPASy TrEMBL
Match: A0A0A0K8Y2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G434960 PE=4 SV=1)

HSP 1 Score: 482 bits (1240), Expect = 2.16e-170
Identity = 236/241 (97.93%), Postives = 239/241 (99.17%), Query Frame = 0

Query: 1   MVCEAKSKVVIPIIDLSKLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFE 60
           MVCEAKSKVVIPIIDLSKLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFE
Sbjct: 1   MVCEAKSKVVIPIIDLSKLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFE 60

Query: 61  ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTT 120
           ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTT
Sbjct: 61  ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTT 120

Query: 121 ENNHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEK 180
           ENNHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEK
Sbjct: 121 ENNHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEK 180

Query: 181 DENDVGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSN 240
           DENDVGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALM  ++
Sbjct: 181 DENDVGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMVTTH 240

BLAST of CsGy7G019270 vs. ExPASy TrEMBL
Match: A0A5A7USF0 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G002400 PE=3 SV=1)

HSP 1 Score: 459 bits (1182), Expect = 1.10e-161
Identity = 225/244 (92.21%), Postives = 231/244 (94.67%), Query Frame = 0

Query: 74  MKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTTENNHFCVAVERFS 133
           MKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQL+ TQSFANLMWPT +NNHFCV VERFS
Sbjct: 1   MKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCVTVERFS 60

Query: 134 TLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKDENDVGLHSHTDL 193
           TLVAKLE MVTKMVLESYGVS KVV+EPIMESTNYLLRLFKYRVAEKDE DVGLHSHTDL
Sbjct: 61  TLVAKLEKMVTKMVLESYGVS-KVVNEPIMESTNYLLRLFKYRVAEKDETDVGLHSHTDL 120

Query: 194 TFLSIIHQHLISGLQIQLL-DDQWLEVHPSSHCSFTVMAGDALMAWSNERIRSCRHRVIM 253
           TF SIIHQHLISGLQIQ L DDQWL+VHPSSHCSF VMAGDALMAWSNERIRSCRH+VIM
Sbjct: 121 TFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCRHKVIM 180

Query: 254 RGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKANASHKTISRIKD 313
           RGEETRYSIGMFSFKNG IEVPQ FVDDANPLRYKPF+HYDFLTYDKANASH TISRIKD
Sbjct: 181 RGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTISRIKD 240

Query: 314 YCGL 316
           YCGL
Sbjct: 241 YCGL 243

BLAST of CsGy7G019270 vs. ExPASy TrEMBL
Match: A0A6J1GPR2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111456352 PE=3 SV=1)

HSP 1 Score: 459 bits (1180), Expect = 2.99e-160
Identity = 221/315 (70.16%), Postives = 256/315 (81.27%), Query Frame = 0

Query: 5   AKSKVVIPIIDLSKLEE---GTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEA 64
           ++++  +P+IDLSKLE+   GT  WG  C+ MR GLE+FGCF VV+++VSLELHNS F A
Sbjct: 3   SETQAGVPVIDLSKLEKLEAGTDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGA 62

Query: 65  TKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTTE 124
             ELF LPIETR+KNTSEK YHGYFG YSFLPLYESMAIDNP QL   QSFANLMWP   
Sbjct: 63  VDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQG 122

Query: 125 NNHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKD 184
           NN F   ++RFSTLVAKL+ +VT+MVLESYGVS +++   I +ST  LLR FKYRV E+ 
Sbjct: 123 NNRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNI-DSTANLLRFFKYRVPEQH 182

Query: 185 ENDVGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSNE 244
           E DVGLH+HTDLTF SIIHQHLI GLQIQ LDDQW+++ PSSHCSF VMAGDALMAWSN+
Sbjct: 183 ETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSND 242

Query: 245 RIRSCRHRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKAN 304
           RIRSCRHRV M+ EETRYS GMFSFKNG I VP+E VDDANPLRYKPF+HYDFLT+DKA 
Sbjct: 243 RIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDKAK 302

Query: 305 ASHKTISRIKDYCGL 316
            S K + RI+DYC +
Sbjct: 303 GSKKFLYRIRDYCSI 316

BLAST of CsGy7G019270 vs. ExPASy TrEMBL
Match: A0A6J1JXN6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111488804 PE=3 SV=1)

HSP 1 Score: 457 bits (1176), Expect = 1.22e-159
Identity = 217/315 (68.89%), Postives = 256/315 (81.27%), Query Frame = 0

Query: 5   AKSKVVIPIIDLSKLEE---GTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEA 64
           ++++  +P+IDLSKLE    GT+ WG  C+ MR GLE+FGCF VV+++VSLELHNS F A
Sbjct: 3   SETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGA 62

Query: 65  TKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTTE 124
             ELF LPIETR+KNTSEK YHGYFG YSFLPLYESMAI+NP QL   QSFANLMWP   
Sbjct: 63  VDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPAQG 122

Query: 125 NNHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKD 184
           NNHF   ++RFSTL+AKL+ ++T+MVLESYGV+ +++   I +ST  LLR FKYRV E+ 
Sbjct: 123 NNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNI-DSTANLLRFFKYRVPEEH 182

Query: 185 ENDVGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSNE 244
           E DVGLH+HTDLTF SIIHQHLI GLQIQ LDDQW+++ PS HCSF VMAGDALMAWSN+
Sbjct: 183 ETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSND 242

Query: 245 RIRSCRHRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKAN 304
           RIRSCRHRV M+ EETRYS GMFSFKNG I VP+E VDDANPLRYKPF+H+DFLTYDKA 
Sbjct: 243 RIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKAK 302

Query: 305 ASHKTISRIKDYCGL 316
            S K + RI+DYC +
Sbjct: 303 GSKKFLYRIRDYCSI 316

BLAST of CsGy7G019270 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 265.4 bits (677), Expect = 5.9e-71
Identity = 130/308 (42.21%), Postives = 196/308 (63.64%), Query Frame = 0

Query: 11  IPIIDLSK--LEEGTKEWGLACREMRFGLEQFGCFSV-VYDKVSLELHNSIFEATKELFH 70
           IP +D S+  L+ GTK W      +R  LE++GCF + + DK  L+L + +F +  +LF 
Sbjct: 5   IPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDLFD 64

Query: 71  LPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTTENNHFCV 130
           LP +T+MKN  +KP +GY G    LPL+ES+ IDN   L+ T+SF  LMWP   N HF  
Sbjct: 65  LPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWP-QGNEHFSE 124

Query: 131 AVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKDENDVGL 190
            + +++   A+L+ MVT+MV +SY V      +P +EST YLLR+ K R    +   +G 
Sbjct: 125 CLYKYAEFAAELDQMVTRMVFQSYNVEK--YYDPYIESTTYLLRVLKNRAPNNENPTLGF 184

Query: 191 HSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSNERIRSCR 250
            +HTD +F +I+HQ  ++GL+++  + + + ++ SS   F V+AGDALMAWSN+R+ S R
Sbjct: 185 VTHTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVWSPR 244

Query: 251 HRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKANASHKTI 310
           H+V++ GE  RYS+GMF+F NG ++VP+E +D  +PL YKPF H   L + + +  +K+ 
Sbjct: 245 HQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGYKSE 304

Query: 311 SRIKDYCG 316
             IK YCG
Sbjct: 305 CPIKAYCG 309

BLAST of CsGy7G019270 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 263.8 bits (673), Expect = 1.7e-70
Identity = 129/309 (41.75%), Postives = 186/309 (60.19%), Query Frame = 0

Query: 11  IPIIDLS---KLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEATKELFH 70
           +PI+DL+   +L+  T  W    RE    LE++GCF  VYD V+ +L +SIF A +ELF 
Sbjct: 10  VPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELFD 69

Query: 71  LPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTTENNHFCV 130
           LP ET+ KN +EKPYHGY G    +PL+E + +D     +  Q F +LMWP   N+ FC 
Sbjct: 70  LPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWP-QGNDRFCN 129

Query: 131 AVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKDENDVGL 190
            V  FS  VA+L+ +V +M+ E+YGV     S   + S  YLL+  KY    +  +    
Sbjct: 130 TVHTFSNAVAELDRLVVRMIFENYGVEKHYESH--VGSKTYLLKFLKYLAPPESISMPAF 189

Query: 191 HSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSNERIRSCR 250
             HTD TFLSI+HQ+ ++GL+++  D +W+ +      S+ VMAGD  M WSN+RIRSC 
Sbjct: 190 PQHTDKTFLSILHQNDVNGLEVKSKDGEWISLQLPPK-SYVVMAGDISMGWSNDRIRSCE 249

Query: 251 HRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKANASHKTI 310
           HRV M G++TRY++G+FSF    + +P+E VDD +PL YKPF +   + +       +  
Sbjct: 250 HRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREAN 309

Query: 311 SRIKDYCGL 317
           S +K YCG+
Sbjct: 310 STLKAYCGI 314

BLAST of CsGy7G019270 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 251.5 bits (641), Expect = 8.8e-67
Identity = 125/310 (40.32%), Postives = 196/310 (63.23%), Query Frame = 0

Query: 11  IPIIDLS--KLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEATKELFHL 70
           +P+ID S   L+ G  EW L   +++  L+ +G F   +D++  EL  S+F A +ELF L
Sbjct: 12  LPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELFDL 71

Query: 71  PIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTTENNHFCVA 130
           P++T+++N S+KP+HGY G Y  +PLYESM ID+    +   +F   +WP   N  F   
Sbjct: 72  PLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWP-QGNISFSTT 131

Query: 131 VERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKDENDVGLH 190
           ++ FS  +++L+  + +M++ES+G+ +K + E  + STNYLLR+ KY+  + +E  VGL+
Sbjct: 132 IQSFSKKLSELDITIRRMIMESFGL-DKYIDEH-LHSTNYLLRVMKYKGPDTEETKVGLN 191

Query: 191 SHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSNERIRSCRH 250
           +HTD   ++I++Q+ + GL++Q  D  W++V P+   SFTVM GD+L A  N R+ S  H
Sbjct: 192 AHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPTQD-SFTVMIGDSLYALLNGRLHSPYH 251

Query: 251 RVIMRGEETRYSIGMFSF-KNGRI-EVPQEFVDDANPLRYKPFHHYDFLTYDKANASHKT 310
           RV+M G ETRYS+G+FS  K G I   P E VD+ +P  +KPF H +FL +    A  ++
Sbjct: 252 RVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAGQRS 311

Query: 311 ISRIKDYCGL 317
            S +K YCG+
Sbjct: 312 QSALKTYCGI 317

BLAST of CsGy7G019270 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 231.5 bits (589), Expect = 9.5e-61
Identity = 128/314 (40.76%), Postives = 178/314 (56.69%), Query Frame = 0

Query: 11  IPIIDLS--KLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEATKELFHL 70
           +PII+LS   L++ T+ W      +R  +E  G F   Y+     LH SI EA KEL  L
Sbjct: 9   LPIINLSDKNLKQNTELWSFTRDSVREAMEHHGWFVAEYNNFPTGLHQSILEAAKELLDL 68

Query: 71  PIETRMKNTSEKPYHGYFGGYS-FLPLYESMAIDNPIQLQPTQSFANLMWPT--TENNHF 130
           P+E ++KN + K  HGY    S   P++E + ID    +Q  + F+ LMWP    +N+ F
Sbjct: 69  PVEIKLKNENHKAGHGYITMMSDGQPVHEGLGIDQVNDVQQCRGFSRLMWPDDHDDNDRF 128

Query: 131 CVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKDENDV 190
           C  V  ++ + A+LE +V +M+ ESY V     +E  +  T YLLRL KYR     E + 
Sbjct: 129 CETVHAYAKMQAELEQLVIRMLFESYNVEK--YTEKYIGGTRYLLRLLKYRRLPNGEPNR 188

Query: 191 GLHSHTDLTFLSIIHQHLISGLQIQL-LDDQWLEVHPSSHCSFTVMAGDALMAWSNERIR 250
              SHTD +F+SI+HQ+ I+GL ++   +D W    PS    F V+AGDA+MAWSN+RI+
Sbjct: 189 KFISHTDKSFISILHQNHITGLMLKSEKEDVWYHFTPSP-TRFVVIAGDAIMAWSNDRIK 248

Query: 251 SCRHRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKANASH 310
           +C H+V M   E RYS+G FSF+ G I  P+E VD  +PL Y PFHH   L Y +    H
Sbjct: 249 ACYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGLLEYYETLEVH 308

Query: 311 KTISR--IKDYCGL 317
               R   K YCG+
Sbjct: 309 LKAHRTMTKAYCGI 319

BLAST of CsGy7G019270 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 226.9 bits (577), Expect = 2.3e-59
Identity = 120/312 (38.46%), Postives = 181/312 (58.01%), Query Frame = 0

Query: 11  IPIIDLS--KLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEATKELFHL 70
           +PI+D S  KL  GT  W     ++R  +E  G F   +  VS +L +++    KE+++L
Sbjct: 9   LPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLLAGMKEMYYL 68

Query: 71  PIETRMKNTSEKPYHGYFG-GYSFLPLYESMAIDNPIQLQPTQSFANLMWPTTENNHFCV 130
           P + ++KN + K  HGY         ++ES+ ID   +LQ  + F+ L+WP   N+ FC 
Sbjct: 69  PDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWP-QGNDPFCQ 128

Query: 131 AVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKDENDVGL 190
               ++  +A+L+  V +M+ ESYG+  K  S    EST YLLR+  YR  +  E + G 
Sbjct: 129 TTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGEANTGF 188

Query: 191 HSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSNERIRSCR 250
            SHTD +F+SI+HQ+ + GLQ++ +  QW+  +PS    F V++G  L AWSN+RI++C 
Sbjct: 189 VSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNPSP-TRFVVLSGMGLTAWSNDRIKACY 248

Query: 251 HRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLT-YDKANASHKT 310
           H+V+M  +E RYS+G FSF  G I  P+E VDD +PLRY PF H   L  Y+    S K 
Sbjct: 249 HKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYESYINSLKK 308

Query: 311 ISR--IKDYCGL 317
            S   ++ YCG+
Sbjct: 309 SSEDLLQIYCGV 318

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q945B61.2e-5739.87Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=... [more]
Q9ZTA34.7e-5739.40Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37... [more]
Q944Z52.0e-5539.00Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
A0A0N9HQ361.1e-4835.08Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=... [more]
Q9ZTA24.9e-3827.74Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thali... [more]
Match NameE-valueIdentityDescription
XP_004136073.12.05e-236100.00probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis sativus][more]
XP_008461057.19.45e-21592.74PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumi... [more]
XP_031744410.12.16e-19385.76probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X2 [Cucumis ... [more]
XP_038898684.13.96e-19383.44probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida][more]
KAA0058792.12.28e-16192.21putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo var.... [more]
Match NameE-valueIdentityDescription
A0A1S3CDV24.58e-21592.74probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX... [more]
A0A0A0K8Y22.16e-17097.93Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G434960 PE=4 SV=1[more]
A0A5A7USF01.10e-16192.21Putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo va... [more]
A0A6J1GPR22.99e-16070.16probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1JXN61.22e-15968.89probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G... [more]
Match NameE-valueIdentityDescription
AT1G52790.15.9e-7142.212-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52800.11.7e-7041.752-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52820.18.8e-6740.322-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G15540.19.5e-6140.762-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G80320.12.3e-5938.462-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 169..266
e-value: 4.2E-16
score: 59.3
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 2..304
e-value: 3.3E-59
score: 202.8
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 11..87
e-value: 3.4E-12
score: 47.2
NoneNo IPR availablePANTHERPTHR47990:SF1692-OXOGLUTARATE-DEPENDENT DIOXYGENASEcoord: 4..315
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 4..315
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 10..297
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 165..269
score: 10.317716

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy7G019270.1CsGy7G019270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding