CsGy7G019260 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy7G019260
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationGy14Chr7: 21893274 .. 21895792 (+)
RNA-Seq ExpressionCsGy7G019260
SyntenyCsGy7G019260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTAAATCTTCTGTCTAATGCTTCTAATTTGAACATAAAACAATAAACCAAAATATCATCAAAGGTTAAAAGGCATCAGAGAGAATAATTAATAAGGTGTTTGGTTAATGGGTTCAGAAACGACGGAGGAGATCAGGCTGCCGGTGATAAATTTCAGTGATGAAAAAGTGAAGGCGGAGGGCGGCGTCGAGTGGCGGAGAACGAGCAAAAATGTCCGGGAAGCCATGGAAGAGTACGGATGTTTTATAGCGAAGTATGAGGGAGTAAGTAAAGAACTTAGGGAGGAAGTGTTGAAGGCAGTGAAAGAACTTTTTGAGCTTCCAATTGAGAGGAAGAAGATGAATAAATATGAAAAGCCTTTTAATGGATACGTTGGAGAGCTTCGTACTCTTCCTCTTCATGAAAGCTTGGGAATTGATGATGCTACTAACTTCAATCAAGTTCAATCCTTCTCTCATCTCATGTGGCCGCCGGAGGGAAATCACCATTTTAGGTCATTTTCTCTTCTTCTTTTACATCTTTCTTTCTTTCAATTTTTCGTTTTTCAGATAGTTAGCAAATTTTACATAAGATGTTCAAATAATCTACGTCTCATGCTCGTTATGCTAAAAAAAAGTTTACGGATAAATCTTATGCTTTTTATACGATTTGATGAACTTATTAGTAATCTTATTGTCAATTGGTATATGAACTAATGTTATTTAATATAAGATATTTTAAAAATGATAAAATATTAAAACTAATTACAAAATATAACAAAATTCATCAAACTCTTAAATACATTTTGTAACTAATTTTAGTATTTTGCTATGGAGAACAATTTTTCTTAATATATGAACCCTAAACCCTTAATATTTTGAGGATGTGAATTGGATCACGTATCATCTGATATGTAAATATTTGTTTTAGCAAGATATAACAAAATTTCACATACCTCAAATAGAAATATGAAATTTTGTTGTACTGTTTTTTTTTATAATTTTTTTTAACACTATTACAACAGAATGTTATTATATATATTTTTTCTGTGGGTGCTGACTTTTATAAATGAGTTGGGATTATTGGATGTTTACTTTGTGGGTTTTTGAGTACTGATCTTGGGAGCTAGCTGCTTTATTATTAGTGGGTTGGTCTCAATAAAGAATTCTTAGCTTCTTGGAGAATGTTGTTCAAATGGATCCAAACAAGAGACAAGACTAGGTAGCCTAGCTAGTTCAATAATAATGAAAGATAAATGAAAGAATTGGACAAAGATAAAGCTTCTTATTTGGAGGGGTTTGGATACATAATTGTTTTGGTTGAAGAGAGAAGTGATAATAATGTTCTTAATTTTTGGTTTGTTTTTTCAGTGCAACTTTGCATTCATTTGCAAAGATAGCAGAAGAATTGGACAAAATGGTGACCAAAATGATATTTGAAAGCTATGGTGTGGAAAAGTATTACAATTCTCATGCTGATTCCATTACTTATCTTCTTAGAGTTTTAAAAAGCAAAGCTCCTCAATCTCCAGACCCTACTTTATGTCTTGTGGATCACACTGATAAGAGCTTCACCACCATGGTCTATCAAGATCATATCAATGGTTTGGAGCTCAAAACCAAAAATGGCCAATGGATTCAAGTTGACAACTCTTCACCCTCAAGCTTCCTCGTCATTGCTGGAGACGCAATCAAGGTTGAATATATCATTATACATATCATACAATTTTTGAATTTTAATGACATTGATGAACTCAAAGTTGAAAGACTAAACTTTTAATAATTTAGTAAAATTCATTCCTACATTCTTATATTATTATCATCAAATCGACAAATAAAGAAAATAAAAATTGTAAACAATATAGAATCGATGAAAAAAGTTAATATAGTGGACTCTAACAAAAGATAAATATATCTATAACGTTAAATAGATATGTAAATTCTCTGCTATTTGTCGATTTCATGATCATTATATTTTGAGAATAAACCCTAATAATTGTATACTGTTTTCTTTTTTTGTTGGGTTGAGAAGGCATGGAGCAATGATAGAATAGAATCACCAACTCATAAAGTGACGATAAGAGGAAATGAAGATAGGTATTCTCTTGCGATGTTTGGATTCAGCAATGGAATTGTGGAAGTGCCAAAAGAACTTGTTGATCAAAATCATCCTTTGCGATATAAACCTTTTGATCATATTGGATTGCTTCATTTCTTCCGTTCTAAAGAGGGCTTCAACTCCAAATCTCCTCTTAAAGACTATTGTGCAATCTAAAATATAATATAACAATGCAATAAGAGTACTCTATCCAGTTTGGTATATGTTTTATATTTGTAAGTTCCTATTATGGGTGTGCTTGTATAAGCTTTGGGTTTTGGTTTGTTTTTCTTTGTTGTGCTTTTAGTTTAAGAACTTTCTCCCCTAATAAACTTCTTGTAACTTTTGTTCTCTTCTTAATATATATGAACAATGTATTCTCTTTTCCTACTCCTCTTACATTTGTGGTTTGTTTTTTAATTCAAAATTTCAAATTAAGAAC

mRNA sequence

TTTTAAATCTTCTGTCTAATGCTTCTAATTTGAACATAAAACAATAAACCAAAATATCATCAAAGGTTAAAAGGCATCAGAGAGAATAATTAATAAGGTGTTTGGTTAATGGGTTCAGAAACGACGGAGGAGATCAGGCTGCCGGTGATAAATTTCAGTGATGAAAAAGTGAAGGCGGAGGGCGGCGTCGAGTGGCGGAGAACGAGCAAAAATGTCCGGGAAGCCATGGAAGAGTACGGATGTTTTATAGCGAAGTATGAGGGAGTAAGTAAAGAACTTAGGGAGGAAGTGTTGAAGGCAGTGAAAGAACTTTTTGAGCTTCCAATTGAGAGGAAGAAGATGAATAAATATGAAAAGCCTTTTAATGGATACGTTGGAGAGCTTCGTACTCTTCCTCTTCATGAAAGCTTGGGAATTGATGATGCTACTAACTTCAATCAAGTTCAATCCTTCTCTCATCTCATGTGGCCGCCGGAGGGAAATCACCATTTTAGTGCAACTTTGCATTCATTTGCAAAGATAGCAGAAGAATTGGACAAAATGGTGACCAAAATGATATTTGAAAGCTATGGTGTGGAAAAGTATTACAATTCTCATGCTGATTCCATTACTTATCTTCTTAGAGTTTTAAAAAGCAAAGCTCCTCAATCTCCAGACCCTACTTTATGTCTTGTGGATCACACTGATAAGAGCTTCACCACCATGGTCTATCAAGATCATATCAATGGTTTGGAGCTCAAAACCAAAAATGGCCAATGGATTCAAGTTGACAACTCTTCACCCTCAAGCTTCCTCGTCATTGCTGGAGACGCAATCAAGGCATGGAGCAATGATAGAATAGAATCACCAACTCATAAAGTGACGATAAGAGGAAATGAAGATAGGTATTCTCTTGCGATGTTTGGATTCAGCAATGGAATTGTGGAAGTGCCAAAAGAACTTGTTGATCAAAATCATCCTTTGCGATATAAACCTTTTGATCATATTGGATTGCTTCATTTCTTCCGTTCTAAAGAGGGCTTCAACTCCAAATCTCCTCTTAAAGACTATTGTGCAATCTAAAATATAATATAACAATGCAATAAGAGTACTCTATCCAGTTTGGTATATGTTTTATATTTGTAAGTTCCTATTATGGGTGTGCTTGTATAAGCTTTGGGTTTTGGTTTGTTTTTCTTTGTTGTGCTTTTAGTTTAAGAACTTTCTCCCCTAATAAACTTCTTGTAACTTTTGTTCTCTTCTTAATATATATGAACAATGTATTCTCTTTTCCTACTCCTCTTACATTTGTGGTTTGTTTTTTAATTCAAAATTTCAAATTAAGAAC

Coding sequence (CDS)

ATGGGTTCAGAAACGACGGAGGAGATCAGGCTGCCGGTGATAAATTTCAGTGATGAAAAAGTGAAGGCGGAGGGCGGCGTCGAGTGGCGGAGAACGAGCAAAAATGTCCGGGAAGCCATGGAAGAGTACGGATGTTTTATAGCGAAGTATGAGGGAGTAAGTAAAGAACTTAGGGAGGAAGTGTTGAAGGCAGTGAAAGAACTTTTTGAGCTTCCAATTGAGAGGAAGAAGATGAATAAATATGAAAAGCCTTTTAATGGATACGTTGGAGAGCTTCGTACTCTTCCTCTTCATGAAAGCTTGGGAATTGATGATGCTACTAACTTCAATCAAGTTCAATCCTTCTCTCATCTCATGTGGCCGCCGGAGGGAAATCACCATTTTAGTGCAACTTTGCATTCATTTGCAAAGATAGCAGAAGAATTGGACAAAATGGTGACCAAAATGATATTTGAAAGCTATGGTGTGGAAAAGTATTACAATTCTCATGCTGATTCCATTACTTATCTTCTTAGAGTTTTAAAAAGCAAAGCTCCTCAATCTCCAGACCCTACTTTATGTCTTGTGGATCACACTGATAAGAGCTTCACCACCATGGTCTATCAAGATCATATCAATGGTTTGGAGCTCAAAACCAAAAATGGCCAATGGATTCAAGTTGACAACTCTTCACCCTCAAGCTTCCTCGTCATTGCTGGAGACGCAATCAAGGCATGGAGCAATGATAGAATAGAATCACCAACTCATAAAGTGACGATAAGAGGAAATGAAGATAGGTATTCTCTTGCGATGTTTGGATTCAGCAATGGAATTGTGGAAGTGCCAAAAGAACTTGTTGATCAAAATCATCCTTTGCGATATAAACCTTTTGATCATATTGGATTGCTTCATTTCTTCCGTTCTAAAGAGGGCTTCAACTCCAAATCTCCTCTTAAAGACTATTGTGCAATCTAA

Protein sequence

MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREEVLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMWPPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQSPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAWSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSKEGFNSKSPLKDYCAI*
Homology
BLAST of CsGy7G019260 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 6.8e-64
Identity = 129/314 (41.08%), Postives = 195/314 (62.10%), Query Frame = 0

Query: 10  RLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREEVLKAVKELF 69
           +LPVI+FSD+ +K  G  +W     +V +A+E+YGCF A ++ +S EL   V +A+++LF
Sbjct: 13  QLPVIDFSDQNLK-PGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLF 72

Query: 70  ELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMWPPEGNHHFS 129
           ELPI  K+ N   KPF+GY+       L+ESLGIDDA    +V  F+  +WP  GN   S
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCH----NLYESLGIDDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 130 ATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQSPD-----P 189
            T+H F++   ELD MV +MI ES+G+E Y + H +S  YL R++K  +P   D      
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEET 192

Query: 190 TLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAWSNDRI 249
            L L  HTDK+  T+++Q  ++GLE+KTK+ +WI+V   S  S LV+ GD++ A  N R+
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKV-KPSQDSVLVMVGDSLCALLNGRL 252

Query: 250 ESPTHKVTIRGNEDRYSLAMFGF--SNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSK 309
            SP H+V + G + RYS  +F    +  I++ P+ELVD+ HP  +KPF++   LHFF+++
Sbjct: 253 HSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTE 312

Query: 310 EGFNSKSPLKDYCA 317
            G  ++S L  + A
Sbjct: 313 AGRIAQSALHAFAA 320

BLAST of CsGy7G019260 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.2e-63
Identity = 128/315 (40.63%), Postives = 196/315 (62.22%), Query Frame = 0

Query: 10  RLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREEVLKAVKELF 69
           +LPVI+FSD+ +K  G  +W   + +V +A+E+YGCF A ++ +S EL   V +A+++LF
Sbjct: 13  QLPVIDFSDQNLK-PGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF 72

Query: 70  ELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMWPPEGNHHFS 129
           ELPI  K+ N   KPF+GY+       L+ESLGI+DA    +V  F+  +WP  GN   S
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 130 ATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQSPD------ 189
            T+H F++   ELD MV +MI ES+G+E Y + H +S  YL R++K  +P   D      
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 190 PTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAWSNDR 249
             L L  HTDK+  T+++Q  ++GLE+KTK+ +WI+V   S  S LV+ GD++ A  N R
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKV-KPSQDSVLVMVGDSLCALLNGR 252

Query: 250 IESPTHKVTIRGNEDRYSLAMFGF--SNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRS 309
           + SP H+V + G + RYS  +F    +  I++ P+ELVD+ HP  +KPF++   LHFF++
Sbjct: 253 LHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQT 312

Query: 310 KEGFNSKSPLKDYCA 317
           + G  ++S L  + A
Sbjct: 313 EAGRIAQSALHAFAA 321

BLAST of CsGy7G019260 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 4.9e-62
Identity = 125/301 (41.53%), Postives = 190/301 (63.12%), Query Frame = 0

Query: 10  RLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREEVLKAVKELF 69
           +LPVI+FSD+ +K  G  +W   + +V +A+E+YGCF A ++ +S EL   V +A+++LF
Sbjct: 6   QLPVIDFSDQNLK-PGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF 65

Query: 70  ELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMWPPEGNHHFS 129
           ELPI  K+ N   K F+GY+       L+ESLGI+DA    +V  F+  +WP  GN   S
Sbjct: 66  ELPIPTKQRNVSSKLFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 125

Query: 130 ATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQSPD----PT 189
            T+H F++   ELD MV +MI ES+G+EKY + H +S  YL R++K  +P   D      
Sbjct: 126 ETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEETK 185

Query: 190 LCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAWSNDRIE 249
           L L  HTDK+  T+++Q  ++GLE+KTK+ +WI+V   S  S LV+ GD++ A  N R+ 
Sbjct: 186 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKV-KPSQDSVLVMVGDSLCALLNGRLH 245

Query: 250 SPTHKVTIRGNEDRYSLAMFGF--SNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSKE 305
           SP H+V + G + RYS  +F    +  I++ P+ELVD+ HP  +KPF++   LHFF+++ 
Sbjct: 246 SPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTEA 300

BLAST of CsGy7G019260 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 4.6e-60
Identity = 125/317 (39.43%), Postives = 188/317 (59.31%), Query Frame = 0

Query: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREE 60
           MGS  T  +RLPVI+ S + +K  G   W      VREA+EEYGCF A  + VS EL++ 
Sbjct: 1   MGS--TAPLRLPVIDLSMKNLK-PGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKA 60

Query: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLP-LHESLGIDDATNFNQVQSFSHLM 120
           V     EL  LP+E K +N  +  ++G+     ++P L+E +G+   T+  +V+ F++LM
Sbjct: 61  VCNKGHELLNLPLETKMLNGNKPEYDGFT----SIPNLNEGMGVGRITDLEKVERFTNLM 120

Query: 121 WPPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAP 180
           W PEGN  F  T++S+ K   E+D ++  M+FES+G+EK+++S  +S  YLL  ++ + P
Sbjct: 121 W-PEGNKDFCETVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQP 180

Query: 181 QSPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAW 240
                +  L  H DKS  T+V Q+ + GLE +TK+G+WI     +  + +V+ GD   AW
Sbjct: 181 GKDGRSPALSLHKDKSILTIVNQNDVKGLEFETKDGEWIL---PTADNHIVLLGDCFMAW 240

Query: 241 SNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFF 300
           SN R+ SP H+VT+  N+ R S + F F   I+E P ELVD+ HPL + PF+   LL + 
Sbjct: 241 SNGRLHSPLHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYC 300

Query: 301 RSKEGFNSKSPLKDYCA 317
            +KEG  +   LK Y A
Sbjct: 301 FTKEGAKAVCDLKQYKA 306

BLAST of CsGy7G019260 vs. ExPASy Swiss-Prot
Match: Q9ZTA1 (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP3 PE=2 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 3.0e-51
Identity = 133/407 (32.68%), Postives = 192/407 (47.17%), Query Frame = 0

Query: 10  RLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREEVLKAVKELF 69
           +LP+I  SD+ +K  G  +W +   +VR+A+E+YGCF AK + VS EL+  VLKA++ELF
Sbjct: 7   QLPLICLSDQTLK-PGSSKWVKVRSDVRKALEDYGCFEAKIDQVSMELQGSVLKAMQELF 66

Query: 70  ELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMWPPEGNHHFS 129
            LP E K+ N   KPF GY   L    L ES GI DA    +   F+  +W PEGN   S
Sbjct: 67  ALPTEAKQRNVCPKPFTGY---LSHNGLSESFGIKDANILEKAHEFTQQLW-PEGNKSIS 126

Query: 130 ATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQSPDPT---- 189
             +  +A+   ELD MV ++I ESYG+E + + H +S  Y +R++K  A    D T    
Sbjct: 127 KMIQLYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIARPDNDITAAVG 186

Query: 190 ------------------------------------------------------------ 249
                                                                       
Sbjct: 187 ANVDNGANDNADGDANVNDDGASIGVKVNVDVGDDVNDNDSVNIGVGVDINVETNVNGDL 246

Query: 250 --------------------------------LCLVDHTDKSFTTMVYQDHINGLELKTK 309
                                           L L  HTDKS T ++YQ  I+GLE+KTK
Sbjct: 247 DAEANGDATAWVVGAVSGNASVGAKEANVDAELGLPSHTDKSLTGIIYQHQIDGLEVKTK 306

Query: 310 NGQWIQVDNSSPSSFLVIAGDAIKAWSNDRIESPTHKVTI-RGNEDRYSLAMFG--FSNG 318
            G+WI+V   +P++ +VIAGDA+ A  N RI SP H+V +    + RY+ A+F       
Sbjct: 307 EGKWIRV-KPAPNTVIVIAGDALCALMNGRIPSPYHRVRVTEKKKTRYAAALFSNPKEGY 366

BLAST of CsGy7G019260 vs. NCBI nr
Match: XP_004136074.1 (2-oxoglutarate-dependent dioxygenase AOP2 isoform X1 [Cucumis sativus] >KAE8646455.1 hypothetical protein Csa_015846 [Cucumis sativus])

HSP 1 Score: 646 bits (1666), Expect = 6.11e-234
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREE 60
           MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREE
Sbjct: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREE 60

Query: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW 120
           VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW
Sbjct: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW 120

Query: 121 PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ 180
           PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ
Sbjct: 121 PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ 180

Query: 181 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAWS 240
           SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAWS
Sbjct: 181 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAWS 240

Query: 241 NDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFR 300
           NDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFR
Sbjct: 241 NDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFR 300

Query: 301 SKEGFNSKSPLKDYCAI 317
           SKEGFNSKSPLKDYCAI
Sbjct: 301 SKEGFNSKSPLKDYCAI 317

BLAST of CsGy7G019260 vs. NCBI nr
Match: XP_038896850.1 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 [Benincasa hispida])

HSP 1 Score: 557 bits (1435), Expect = 8.51e-199
Identity = 274/317 (86.44%), Postives = 292/317 (92.11%), Query Frame = 0

Query: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREE 60
           MGSET     LPVINFS   VK EGG EWRR S+ VREA+EEYGCFIA+Y+GVS+ELR E
Sbjct: 1   MGSETGG---LPVINFSGNGVKTEGGDEWRRRSREVREALEEYGCFIAEYDGVSRELRGE 60

Query: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW 120
           V+KAVK LFELPIERKK NKYEKPFNGYVGELRTLPLHESLGIDDATNF+ VQSF+HLMW
Sbjct: 61  VMKAVKALFELPIERKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVQSFTHLMW 120

Query: 121 PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ 180
           P  GNHHFS TLHSFAKIAEELDKMVTKMIFESYGVEKY+ SHADSITYLLR+LK+KAP 
Sbjct: 121 P-SGNHHFSTTLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAPH 180

Query: 181 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAWS 240
           S +PTLCLVDHTDKSFTTM+YQDHINGLE+KTK+GQWIQVDNSSPS+F+VIAGDA KAWS
Sbjct: 181 SSNPTLCLVDHTDKSFTTMLYQDHINGLEVKTKHGQWIQVDNSSPSTFVVIAGDAFKAWS 240

Query: 241 NDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFR 300
           NDRIESPTHKV IRGNEDRYSLAMFGFSNGIVEVPKELVDQ HPLRYKPFDHIGLLHFFR
Sbjct: 241 NDRIESPTHKVMIRGNEDRYSLAMFGFSNGIVEVPKELVDQTHPLRYKPFDHIGLLHFFR 300

Query: 301 SKEGFNSKSPLKDYCAI 317
           SKEGFNSKSPLKDYCA+
Sbjct: 301 SKEGFNSKSPLKDYCAL 313

BLAST of CsGy7G019260 vs. NCBI nr
Match: XP_031744406.1 (2-oxoglutarate-dependent dioxygenase AOP3 isoform X2 [Cucumis sativus])

HSP 1 Score: 541 bits (1395), Expect = 2.51e-193
Identity = 274/317 (86.44%), Postives = 274/317 (86.44%), Query Frame = 0

Query: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREE 60
           MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREE
Sbjct: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREE 60

Query: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW 120
           VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW
Sbjct: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW 120

Query: 121 PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ 180
           PPEGNHHF                                           RVLKSKAPQ
Sbjct: 121 PPEGNHHF-------------------------------------------RVLKSKAPQ 180

Query: 181 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAWS 240
           SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAWS
Sbjct: 181 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAWS 240

Query: 241 NDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFR 300
           NDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFR
Sbjct: 241 NDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFR 274

Query: 301 SKEGFNSKSPLKDYCAI 317
           SKEGFNSKSPLKDYCAI
Sbjct: 301 SKEGFNSKSPLKDYCAI 274

BLAST of CsGy7G019260 vs. NCBI nr
Match: XP_008461058.1 (PREDICTED: probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X2 [Cucumis melo])

HSP 1 Score: 533 bits (1372), Expect = 3.76e-189
Identity = 263/317 (82.97%), Postives = 282/317 (88.96%), Query Frame = 0

Query: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREE 60
           MGSET EE RLPVINFSDEKVKAEG VEWRRTSK VREA+EEYGCFIAKYEGV KELREE
Sbjct: 1   MGSETMEE-RLPVINFSDEKVKAEGSVEWRRTSKKVREALEEYGCFIAKYEGVGKELREE 60

Query: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW 120
           VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFN VQSFSHLMW
Sbjct: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNGVQSFSHLMW 120

Query: 121 PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ 180
           P EGNHHFSATLHSFAK AEELDKMVTKMIFESYGVEKYYNSHADSITYLLR+LK+KAPQ
Sbjct: 121 P-EGNHHFSATLHSFAKRAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRILKNKAPQ 180

Query: 181 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAWS 240
           SPDPTLCLVDHTDKSFTTM+YQDHINGLELKTKNGQWIQVDNSSPSSF+VIAGDA KAWS
Sbjct: 181 SPDPTLCLVDHTDKSFTTMIYQDHINGLELKTKNGQWIQVDNSSPSSFVVIAGDAFKAWS 240

Query: 241 NDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFR 300
           N+RI S  H+V +RG E RYS+ MF F NG +EVP+  VD  +PLRYKPF+H   L + +
Sbjct: 241 NERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDK 300

Query: 301 SKEGFNSKSPLKDYCAI 317
           +    N+ S +KDYC +
Sbjct: 301 ANASHNTISRIKDYCGL 315

BLAST of CsGy7G019260 vs. NCBI nr
Match: XP_022953974.1 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 [Cucurbita moschata])

HSP 1 Score: 529 bits (1363), Expect = 9.51e-188
Identity = 260/319 (81.50%), Postives = 285/319 (89.34%), Query Frame = 0

Query: 1   MGSETTEEIR--LPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELR 60
           MGSET   +   LPVINFS + VKAEGG  WRR S  VREA+EEYGCFIA+YEG+S ELR
Sbjct: 1   MGSETGPRMDAGLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELR 60

Query: 61  EEVLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHL 120
            EV KAV+ LFELPIE KK N+YEKPFNGYVGELRTLPLHESLGIDDATN + VQ+F+HL
Sbjct: 61  VEVFKAVEALFELPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIDGVQAFTHL 120

Query: 121 MWPPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKA 180
           MWP  GNHHFS+ LHSFAK AEELD+MVTKMIFESYGVEKY+ +HA SITYLLR LK+KA
Sbjct: 121 MWP-NGNHHFSSNLHSFAKKAEELDQMVTKMIFESYGVEKYHETHAASITYLLRALKNKA 180

Query: 181 PQSPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKA 240
           P S +PTLCLVDHTDKSFTTMVYQDH NGLE++TK+G+WIQVDNSSPS+F+VIAGDA KA
Sbjct: 181 PHSQNPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFKA 240

Query: 241 WSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHF 300
           WSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKEL+DQ HPLRYKPFDHIGLLHF
Sbjct: 241 WSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELIDQTHPLRYKPFDHIGLLHF 300

Query: 301 FRSKEGFNSKSPLKDYCAI 317
           FRSKEGF SKSPL+DYCA+
Sbjct: 301 FRSKEGFKSKSPLQDYCAL 318

BLAST of CsGy7G019260 vs. ExPASy TrEMBL
Match: A0A1S3CDV1 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499759 PE=3 SV=1)

HSP 1 Score: 533 bits (1372), Expect = 1.82e-189
Identity = 263/317 (82.97%), Postives = 282/317 (88.96%), Query Frame = 0

Query: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREE 60
           MGSET EE RLPVINFSDEKVKAEG VEWRRTSK VREA+EEYGCFIAKYEGV KELREE
Sbjct: 1   MGSETMEE-RLPVINFSDEKVKAEGSVEWRRTSKKVREALEEYGCFIAKYEGVGKELREE 60

Query: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW 120
           VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFN VQSFSHLMW
Sbjct: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNGVQSFSHLMW 120

Query: 121 PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ 180
           P EGNHHFSATLHSFAK AEELDKMVTKMIFESYGVEKYYNSHADSITYLLR+LK+KAPQ
Sbjct: 121 P-EGNHHFSATLHSFAKRAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRILKNKAPQ 180

Query: 181 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAWS 240
           SPDPTLCLVDHTDKSFTTM+YQDHINGLELKTKNGQWIQVDNSSPSSF+VIAGDA KAWS
Sbjct: 181 SPDPTLCLVDHTDKSFTTMIYQDHINGLELKTKNGQWIQVDNSSPSSFVVIAGDAFKAWS 240

Query: 241 NDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFR 300
           N+RI S  H+V +RG E RYS+ MF F NG +EVP+  VD  +PLRYKPF+H   L + +
Sbjct: 241 NERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDK 300

Query: 301 SKEGFNSKSPLKDYCAI 317
           +    N+ S +KDYC +
Sbjct: 301 ANASHNTISRIKDYCGL 315

BLAST of CsGy7G019260 vs. ExPASy TrEMBL
Match: A0A6J1GRH7 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Cucurbita moschata OX=3662 GN=LOC111456372 PE=4 SV=1)

HSP 1 Score: 529 bits (1363), Expect = 4.60e-188
Identity = 260/319 (81.50%), Postives = 285/319 (89.34%), Query Frame = 0

Query: 1   MGSETTEEIR--LPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELR 60
           MGSET   +   LPVINFS + VKAEGG  WRR S  VREA+EEYGCFIA+YEG+S ELR
Sbjct: 1   MGSETGPRMDAGLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELR 60

Query: 61  EEVLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHL 120
            EV KAV+ LFELPIE KK N+YEKPFNGYVGELRTLPLHESLGIDDATN + VQ+F+HL
Sbjct: 61  VEVFKAVEALFELPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIDGVQAFTHL 120

Query: 121 MWPPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKA 180
           MWP  GNHHFS+ LHSFAK AEELD+MVTKMIFESYGVEKY+ +HA SITYLLR LK+KA
Sbjct: 121 MWP-NGNHHFSSNLHSFAKKAEELDQMVTKMIFESYGVEKYHETHAASITYLLRALKNKA 180

Query: 181 PQSPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKA 240
           P S +PTLCLVDHTDKSFTTMVYQDH NGLE++TK+G+WIQVDNSSPS+F+VIAGDA KA
Sbjct: 181 PHSQNPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFKA 240

Query: 241 WSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHF 300
           WSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKEL+DQ HPLRYKPFDHIGLLHF
Sbjct: 241 WSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELIDQTHPLRYKPFDHIGLLHF 300

Query: 301 FRSKEGFNSKSPLKDYCAI 317
           FRSKEGF SKSPL+DYCA+
Sbjct: 301 FRSKEGFKSKSPLQDYCAL 318

BLAST of CsGy7G019260 vs. ExPASy TrEMBL
Match: A0A6J1JM78 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Cucurbita maxima OX=3661 GN=LOC111488145 PE=4 SV=1)

HSP 1 Score: 525 bits (1353), Expect = 1.54e-186
Identity = 258/319 (80.88%), Postives = 285/319 (89.34%), Query Frame = 0

Query: 1   MGSETTEEIR--LPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELR 60
           MGSET   +   LPVINFS + VKAEGG  WRR S  VREA+EEYGCFIA+YEG+S ELR
Sbjct: 1   MGSETGPRMDAGLPVINFSGDDVKAEGGEVWRRRSGEVREALEEYGCFIAEYEGISPELR 60

Query: 61  EEVLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHL 120
            +V KAV+ LFELPIE KK N+YEKPFNGYVGELRTLPLHESLGIDDATN + VQ+F++L
Sbjct: 61  VQVFKAVEALFELPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIDGVQAFTNL 120

Query: 121 MWPPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKA 180
           MWP  GNHHFS+ LHSFAK AEELD+MVTKMIFESYGVEKY+ +HA SITYLLR LK+KA
Sbjct: 121 MWP-NGNHHFSSNLHSFAKKAEELDQMVTKMIFESYGVEKYHETHAASITYLLRALKNKA 180

Query: 181 PQSPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKA 240
           P S +PTLCLVDHTDKSFTTMVYQDH NGLE++TK+G+WIQVDNSSPS+F+VIAGDA KA
Sbjct: 181 PHSQNPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFKA 240

Query: 241 WSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHF 300
           WSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKEL+DQ HPLRYKPFDHIGLLHF
Sbjct: 241 WSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELIDQTHPLRYKPFDHIGLLHF 300

Query: 301 FRSKEGFNSKSPLKDYCAI 317
           FRSKEGF SKSPL+DYCA+
Sbjct: 301 FRSKEGFKSKSPLQDYCAL 318

BLAST of CsGy7G019260 vs. ExPASy TrEMBL
Match: A0A6J1DBW9 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=3673 GN=LOC111019607 PE=3 SV=1)

HSP 1 Score: 503 bits (1295), Expect = 1.93e-177
Identity = 242/309 (78.32%), Postives = 277/309 (89.64%), Query Frame = 0

Query: 9   IRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREEVLKAVKEL 68
           + LPVIN S + ++  GG  W+ T K VREA+EEYGCF+A+Y GVS ELRE+V KA+++L
Sbjct: 29  VGLPVINLSGDDLRP-GGERWKTTGKKVREAVEEYGCFMAEYNGVSPELREQVFKALEQL 88

Query: 69  FELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMWPPEGNHHF 128
           F+LP+ERK  NKYEKPFNGYVG+LRTLPLHESLGIDDAT F+ V SF++LMWP  GNHHF
Sbjct: 89  FDLPVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWP-SGNHHF 148

Query: 129 SATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQSPDPTLCL 188
           SATLHSFAKIAE LDKMVTKMIFESYGVEKYY+SHA SITYLLRVLK+KAPQS +P+LCL
Sbjct: 149 SATLHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCL 208

Query: 189 VDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAWSNDRIESPT 248
           VDHTDKSFTTM+YQDHINGLE+KTK+GQWI+V+ SSPS+F+VIAGDA KAWSN RIESPT
Sbjct: 209 VDHTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPT 268

Query: 249 HKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSKEGFNSK 308
           HKV  RGNEDRYS+ MFGFSNG VEVPKELVDQNHPL+YKPF+HIGLL FF+S+EGFNSK
Sbjct: 269 HKVMTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSK 328

Query: 309 SPLKDYCAI 317
           SPLKDYCA+
Sbjct: 329 SPLKDYCAL 335

BLAST of CsGy7G019260 vs. ExPASy TrEMBL
Match: A0A0A0K746 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G434950 PE=4 SV=1)

HSP 1 Score: 475 bits (1222), Expect = 1.92e-167
Identity = 237/237 (100.00%), Postives = 237/237 (100.00%), Query Frame = 0

Query: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREE 60
           MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREE
Sbjct: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREE 60

Query: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW 120
           VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW
Sbjct: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW 120

Query: 121 PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ 180
           PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ
Sbjct: 121 PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ 180

Query: 181 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIK 237
           SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIK
Sbjct: 181 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIK 237

BLAST of CsGy7G019260 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 360.9 bits (925), Expect = 1.0e-99
Identity = 164/307 (53.42%), Postives = 231/307 (75.24%), Query Frame = 0

Query: 10  RLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSK-ELREEVLKAVKEL 69
           ++P ++FS E +K  G   W  T +N+R+A+EEYGCFI   +  +  +L + V  ++ +L
Sbjct: 4   KIPTLDFSREDLK-PGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDL 63

Query: 70  FELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMWPPEGNHHF 129
           F+LP + K  NKY+KP NGYVG++  LPLHESLGID+AT+    +SF+ LMW P+GN HF
Sbjct: 64  FDLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMW-PQGNEHF 123

Query: 130 SATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQSPDPTLCL 189
           S  L+ +A+ A ELD+MVT+M+F+SY VEKYY+ + +S TYLLRVLK++AP + +PTL  
Sbjct: 124 SECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPTLGF 183

Query: 190 VDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAWSNDRIESPT 249
           V HTDKSFTT+++QD +NGLE++T+ G+ I ++ SSPS F+V+AGDA+ AWSNDR+ SP 
Sbjct: 184 VTHTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVWSPR 243

Query: 250 HKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSKEGFNSK 309
           H+V + G  DRYSL MF F+NG ++VP+EL+D  HPL YKPFDH+GLL F+R+  G+ S+
Sbjct: 244 HQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGYKSE 303

Query: 310 SPLKDYC 316
            P+K YC
Sbjct: 304 CPIKAYC 308

BLAST of CsGy7G019260 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 317.4 bits (812), Expect = 1.3e-86
Identity = 144/308 (46.75%), Postives = 210/308 (68.18%), Query Frame = 0

Query: 10  RLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREEVLKAVKELF 69
           ++P+++ + ++        WR  S+   EA+EEYGCF+A Y+GV+++L + +  A +ELF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 70  ELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMWPPEGNHHFS 129
           +LP E KK N  EKP++GYVG++  +PLHE LG+D  TN    Q F+HLMW P+GN  F 
Sbjct: 69  DLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMW-PQGNDRFC 128

Query: 130 ATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQSPDPTLCLV 189
            T+H+F+    ELD++V +MIFE+YGVEK+Y SH  S TYLL+ LK  AP          
Sbjct: 129 NTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPESISMPAFP 188

Query: 190 DHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAWSNDRIESPTH 249
            HTDK+F ++++Q+ +NGLE+K+K+G+WI +    P S++V+AGD    WSNDRI S  H
Sbjct: 189 QHTDKTFLSILHQNDVNGLEVKSKDGEWISL-QLPPKSYVVMAGDISMGWSNDRIRSCEH 248

Query: 250 KVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSKEGFNSKS 309
           +VT+ G++ RY+L +F F   +V +P+ELVD  HPL YKPFD+I L++F+ +KEG  + S
Sbjct: 249 RVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREANS 308

Query: 310 PLKDYCAI 318
            LK YC I
Sbjct: 309 TLKAYCGI 314

BLAST of CsGy7G019260 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 280.0 bits (715), Expect = 2.3e-75
Identity = 142/320 (44.38%), Postives = 211/320 (65.94%), Query Frame = 0

Query: 1   MGSET-TEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELRE 60
           MGSET    +RLPVI+FS++ +K  G  EW  T  +V++A+++YG F A ++ +  ELR+
Sbjct: 1   MGSETPLLPLRLPVIDFSNKNLK-PGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRK 60

Query: 61  EVLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLM 120
            V  A++ELF+LP++ K  N  +KPF+GYVG+   +PL+ES+GIDD+    +V +F+  +
Sbjct: 61  SVFGALEELFDLPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKL 120

Query: 121 WPPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAP 180
           W P+GN  FS T+ SF+K   ELD  + +MI ES+G++KY + H  S  YLLRV+K K P
Sbjct: 121 W-PQGNISFSTTIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGP 180

Query: 181 QSPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAW 240
            + +  + L  HTDK+  T++YQ+H+ GLE++TK+  WI+V   +  SF V+ GD++ A 
Sbjct: 181 DTEETKVGLNAHTDKNIVTILYQNHVEGLEVQTKDKNWIKV-KPTQDSFTVMIGDSLYAL 240

Query: 241 SNDRIESPTHKVTIRGNEDRYSLAMFGF--SNGIVEVPKELVDQNHPLRYKPFDHIGLLH 300
            N R+ SP H+V + G E RYSL +F    +  IV  P ELVD+ HP  +KPFDH+  L 
Sbjct: 241 LNGRLHSPYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQ 300

Query: 301 FFRSKEGFNSKSPLKDYCAI 318
           F+ ++ G  S+S LK YC I
Sbjct: 301 FYYTEAGQRSQSALKTYCGI 317

BLAST of CsGy7G019260 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 261.2 bits (666), Expect = 1.1e-69
Identity = 138/323 (42.72%), Postives = 196/323 (60.68%), Query Frame = 0

Query: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREE 60
           MG   T E  LP+IN SD+ +K    + W  T  +VREAME +G F+A+Y      L + 
Sbjct: 1   MGGNETRE--LPIINLSDKNLKQNTEL-WSFTRDSVREAMEHHGWFVAEYNNFPTGLHQS 60

Query: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRT-LPLHESLGIDDATNFNQVQSFSHLM 120
           +L+A KEL +LP+E K  N+  K  +GY+  +    P+HE LGID   +  Q + FS LM
Sbjct: 61  ILEAAKELLDLPVEIKLKNENHKAGHGYITMMSDGQPVHEGLGIDQVNDVQQCRGFSRLM 120

Query: 121 WPP--EGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSK 180
           WP   + N  F  T+H++AK+  EL+++V +M+FESY VEKY   +     YLLR+LK +
Sbjct: 121 WPDDHDDNDRFCETVHAYAKMQAELEQLVIRMLFESYNVEKYTEKYIGGTRYLLRLLKYR 180

Query: 181 APQSPDPTLCLVDHTDKSFTTMVYQDHINGLELKT-KNGQWIQVDNSSPSSFLVIAGDAI 240
              + +P    + HTDKSF ++++Q+HI GL LK+ K   W      SP+ F+VIAGDAI
Sbjct: 181 RLPNGEPNRKFISHTDKSFISILHQNHITGLMLKSEKEDVWYHF-TPSPTRFVVIAGDAI 240

Query: 241 KAWSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLL 300
            AWSNDRI++  HKV +   E RYSL  F F  G++  P+E+VD++HPL Y PF H GLL
Sbjct: 241 MAWSNDRIKACYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGLL 300

Query: 301 HFFRSKEGF--NSKSPLKDYCAI 318
            ++ + E      ++  K YC I
Sbjct: 301 EYYETLEVHLKAHRTMTKAYCGI 319

BLAST of CsGy7G019260 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 260.8 bits (665), Expect = 1.5e-69
Identity = 136/320 (42.50%), Postives = 199/320 (62.19%), Query Frame = 0

Query: 4   ETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREEVLK 63
           ++ EE  LP+++FS +K+   G   W  T  +VR AME  G F+A++ GVS +LR+ +L 
Sbjct: 2   DSHEEQCLPILDFSSDKL-VRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLLA 61

Query: 64  AVKELFELPIERKKMNKYEKPFNGYVG-ELRTLPLHESLGIDDATNFNQVQSFSHLMWPP 123
            +KE++ LP + K  N+  K  +GY+   +    +HESLGID AT     + FS L+W P
Sbjct: 62  GMKEMYYLPDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLW-P 121

Query: 124 EGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVE--KYYNSHADSITYLLRVLKSKAPQ 183
           +GN  F  T H +A    ELD+ V +M++ESYG++  K+  SH++S  YLLR+L  +  Q
Sbjct: 122 QGNDPFCQTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQ 181

Query: 184 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKAWS 243
           + +     V HTDKSF ++++Q+H+ GL+LKT  GQW+   N SP+ F+V++G  + AWS
Sbjct: 182 NGEANTGFVSHTDKSFMSILHQNHVGGLQLKTMTGQWVGF-NPSPTRFVVLSGMGLTAWS 241

Query: 244 NDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFR 303
           NDRI++  HKV +  +E RYSL  F F  G +  P+ELVD  HPLRY PF+H GLL F+ 
Sbjct: 242 NDRIKACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYE 301

Query: 304 SKEGFNSKSP---LKDYCAI 318
           S      KS    L+ YC +
Sbjct: 302 SYINSLKKSSEDLLQIYCGV 318

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q945B66.8e-6441.08Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=... [more]
Q9ZTA31.2e-6340.63Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37... [more]
Q944Z54.9e-6241.53Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
A0A0N9HQ364.6e-6039.43Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=... [more]
Q9ZTA13.0e-5132.682-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP... [more]
Match NameE-valueIdentityDescription
XP_004136074.16.11e-234100.002-oxoglutarate-dependent dioxygenase AOP2 isoform X1 [Cucumis sativus] >KAE86464... [more]
XP_038896850.18.51e-19986.44probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 [Benincasa hispida][more]
XP_031744406.12.51e-19386.442-oxoglutarate-dependent dioxygenase AOP3 isoform X2 [Cucumis sativus][more]
XP_008461058.13.76e-18982.97PREDICTED: probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X... [more]
XP_022953974.19.51e-18881.50probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A1S3CDV11.82e-18982.97probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X2 OS=Cucumi... [more]
A0A6J1GRH74.60e-18881.50probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Cucurbita moschat... [more]
A0A6J1JM781.54e-18680.88probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Cucurbita maxima ... [more]
A0A6J1DBW91.93e-17778.32probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=367... [more]
A0A0A0K7461.92e-167100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G434950 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G52790.11.0e-9953.422-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52800.11.3e-8646.752-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52820.12.3e-7544.382-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G15540.11.1e-6942.722-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G80320.11.5e-6942.502-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 11..90
e-value: 8.9E-13
score: 49.0
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 2..294
e-value: 5.8E-56
score: 192.4
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 178..266
e-value: 1.1E-14
score: 54.7
NoneNo IPR availablePANTHERPTHR47990:SF1532-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEINcoord: 8..316
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 8..316
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 6..299
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 145..268
score: 8.096536

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy7G019260.1CsGy7G019260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding