CsGy7G018320 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy7G018320
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionpre-mRNA-splicing factor ISY1 homolog
LocationGy14Chr7: 21401183 .. 21404736 (-)
RNA-Seq ExpressionCsGy7G018320
SyntenyCsGy7G018320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GATTGGGTTTGCGCTCGGCGTGCTAAAGTTAGAGCTTCTAGTTCGAGTCTGCCTATTGCCATTTCACCGTCTAAGGAGGAAGAAAAGGAATCGAGGTTTCTTCCGGTGCTACTCGTCGGCCAAATTTTGGTCAGGATATTGTTCGACGGTTAAATTATTCTGGTGCAGGTAGGTTTTCTTCAGCTTAATTTTTATTTCTGTAATTTTTTTCCATTCGAATTGCTCTTCGATTTCGACTTTCAAATCATAGATGACTGATTGATAAACCCTAGCGGCGGCACTTTTATACTTCATCGCAATGTCTCTAGTTTTTCGGTGTTTCTGCTTTCTTTCTTCACCTCTATTCGCTAGGCTCCTAACATCTCTCTCTCGGTCTGGATATCAGCTATGGTTTCATATACCTCGTGGTTGTGCTTACGCTATGATGTTTGTAGGTTCTGACAGTGGTTATTTCTTTTTATGTATCGATTTCTTGATGTTATTTTTCAGATTTGAGGCCAAGTTTCTTGTCTATTTGATATTCGGTTTTCAATTCACTCATACAATGTTGTTTACTTTGATTATTTGTATGTCATTTGGTCAGTGGATTCATGTGTGTATACATATTTGGTGTTATGTTTCACTGAAAGCTAGATAGTCAATTGCTAATGTCGCTGGCTAAAAATTTCTCTAATTAATAGTTCTTTAGCTGTAAAATATTTCTACATTTTCATTTCGTTGTTGATTTACCTGCCTTAGAACTGAGAGGCTGTATGCTATTGACATGAAGCTGTAAGTGTGTGAGCGTGCATGCAAGTTGTGGTGACTGAGATTTAGCTTTTTTGTCACAACAGTCGAACAAATTTACTTTGATCTTATTTATTGAATGGGTTGCTTTGGAAATATGTTGCCTATAAAGTCTATAATTTTAGTTGAACTCAAAGCTGTATGCCAGTTTTGGTCTAGCAATGAATGGATCTGATCTCGGATCACAGATAACAAATATATTTTGGGCTGTCAAGGTGATGGAACAATTATTGATGAAATGAACTGAAACAAATGCATGATGACCATCTTTTTTTTTCTTTTTCTTTTTCTTTTGAGATACTGACTATTTTTTTAACTTTTGGTTGGTTTAACAATTCTTTTTCCATATAGTTCATCGATGAGTTCTCTGTTTTTATTTTTTTAGTGAAATTCTAAACTGTTATTTTCCTTCAAAAAATTGACATGGAAAAACTGGTTCTTGGTGATTGCGATAGACATTATTAGGCGGTATATGAGAAAGAGAAAAAGAGAGAGCAAGTAAAGCATTATAGAATTATGTGGACAATTGGAAAGAAAGGAATAGTAGAATTTTTGAAGATAGATATAATTCTTTTGATTCTTTTTGGACTGTGGTACAATACACAGCTTCTTGGTGGAGTACGAATTACACCAAACACTTTTGTAATTATAGCCTTTCAATGATTTTCAACAATTGGAAGGCTATCATTTCTTAGTTCCTTAGCTTCTTCCAGGGAGGGCCCTCTTGTCCCTCGCCCTTAGGTTGTTCTATTTTGTTATATGAATATACTTGTTTCTTATAAAAAAAAGGAATTATGTGGGCCCCTTTATAAGATTCAGTTTGTTATGAATTAGGCTTTTTTTAAGTCTGGGGAGGGGAGTCTTGTTCATCTTTTTGCACTGTATCGAGTGTAGCTATCAGAGCATTTGAAGGGAGGTAGCCTTGGCGTCCTTGGCATTTTGGCCTAAAGGGTAAATAGATAATCCCAGTTCTATCAGATTGGTTCTGTTACAATTGTCTAAGAAATACCATTCTGTAGGGAAAAAAAAACCTTCTGTATCCAAGTTGCTGCATTATACAGCATTATAGGTTGGTTGGAATCTCTTTTATTAGCCAGTTTCTAACTATTGCTAGATGCAGGTATTCTTTTCTTAGTTTGAATCTTTACTTAGGTTTTGGGGGATTGATAGTCTTATACTGTTTTTTGTAGAGGTACAGCCAGAGAGCCCATCTTTGGAATTTCCTTTGAATTAGGGTAAATGATAGTCCATTTGGAACTTTGTTCCCTCGTTGAGTTTAGAGAGGAAGCCCAGTTCTTCCTTATCAGAGAAGGCTAATTTTTCCTAGTTCGTGGTGTATTTGTAGGGTAGGATACGATGGCTCGAAATGAGGAGAAAGCACAGTCTATGCTGAATCGTTTTATAGCCTTAAAAGCTGAGGAGAAGAAGAAACCCAAGGAACGTCGACCCTACCTTGCCTCTGAATGTCGTGACCTTGCAGAAGCTGACAAATGGCGTCAACAGATCATGCGTGAGATTGGTCGCAAGGTTGCTGAGATCCAAAACGAAGGCCTTGGTGAGCACCGTTTGCGTGATCTCAATGATGAGATTAACAAGCTCATTAGAGAGAAGTCACATTGGGAGCGTCGTATAATTGAGCTTGGTGGCCCCAATTATACCAAACACTCGGCCAAAATGACTGATTTAGATGGGAATATTGTTGATGTACCAAACCCCAGTGGTCGTGGTCCTGGATATCGGTATTTTGGTGCTGCAAAGAAGTTACCAGGTGTTAGGGAATTGTTTGAGAAGCCACCTGAACTGCGGAAGCGCAGGACTCGTTATGACATATACAAGAGGATCGACGCAAGCTACTATGGCTACAGGGACGACGAGGATGGGGTTTTGGAAAGAGTGGAAGGTCCAAGTGAAGAGCGAATGAGAGCTGAGGCAGTTGAAGAGTGGCAAAGAATGGAAGAGATTAGGAAAGAAGCAAAGAGGGCAGTAAAGAGCGGGGAGGTAGCCAGCGTGGTGAATGTTGCAGCAAAGGAGGTACTGTTTGAAGAAGAGGAAGAGGTTGTTGAAGAAGAGAGGAAAAGAGAGAGGGAAATGAAGGAGAATTTGGACAAGGAGAGGGAATTTGTGGTGCATGTGCCATTACCTGATGAAAAGGAGATTGAGAAGATGGTATTGGAGAAGAAGAAGATGGAACTGTTGAGTAAGTATGCGAGTGATATGCTTTTAGAGGAACAGAGTGAAGCCAAATCCATGCTCAATATACAAAGATAACTCCCAAAATCGTCATAGATTTGTTTAATCTATTTTCCTTACTCAAATTCTCCATGCCTATACTATGCACCACACACAGCTTCTGTGGTCAAATGGCGAAGTCATATTCTGGAATCAAGCAGAGTGATGCAAAAATTGAGGTATATGAGGAACTACGTTGTAATGTTTGTTCAGATCTCTCTATATTTTTGGTTTAAACATCTCTTAGAGATACAATCAACGTTGATAACTGATGTAAAACCTTAGATAATAGGATGTTTGAATTTTCCAAACTGATGTAACTTTGAGAGATGTTTTGGGAGCCGCCCTCTCAAGAGTTGATTTACATTTAGATAAAGACACATATTTAGGCTCTTTCCATAAGGTTCTTTGTATTTCCATTTTGCTTCTTTCGGGTTTAGAAACAAGTTTTCTCCAAGCAGAATGTTGACTTCCCATTCTATTACTTTAGGTGCATGAAACGTCTAGCGAAAACATACAACCATATAACAACGAATGCT

mRNA sequence

GATTGGGTTTGCGCTCGGCGTGCTAAAGTTAGAGCTTCTAGTTCGAGTCTGCCTATTGCCATTTCACCGTCTAAGGAGGAAGAAAAGGAATCGAGGTTTCTTCCGGTGCTACTCGTCGGCCAAATTTTGGTCAGGATATTGTTCGACGGTTAAATTATTCTGGTGCAGGGTAGGATACGATGGCTCGAAATGAGGAGAAAGCACAGTCTATGCTGAATCGTTTTATAGCCTTAAAAGCTGAGGAGAAGAAGAAACCCAAGGAACGTCGACCCTACCTTGCCTCTGAATGTCGTGACCTTGCAGAAGCTGACAAATGGCGTCAACAGATCATGCGTGAGATTGGTCGCAAGGTTGCTGAGATCCAAAACGAAGGCCTTGGTGAGCACCGTTTGCGTGATCTCAATGATGAGATTAACAAGCTCATTAGAGAGAAGTCACATTGGGAGCGTCGTATAATTGAGCTTGGTGGCCCCAATTATACCAAACACTCGGCCAAAATGACTGATTTAGATGGGAATATTGTTGATGTACCAAACCCCAGTGGTCGTGGTCCTGGATATCGGTATTTTGGTGCTGCAAAGAAGTTACCAGGTGTTAGGGAATTGTTTGAGAAGCCACCTGAACTGCGGAAGCGCAGGACTCGTTATGACATATACAAGAGGATCGACGCAAGCTACTATGGCTACAGGGACGACGAGGATGGGGTTTTGGAAAGAGTGGAAGGTCCAAGTGAAGAGCGAATGAGAGCTGAGGCAGTTGAAGAGTGGCAAAGAATGGAAGAGATTAGGAAAGAAGCAAAGAGGGCAGTAAAGAGCGGGGAGGTAGCCAGCGTGGTGAATGTTGCAGCAAAGGAGGTACTGTTTGAAGAAGAGGAAGAGGTTGTTGAAGAAGAGAGGAAAAGAGAGAGGGAAATGAAGGAGAATTTGGACAAGGAGAGGGAATTTGTGGTGCATGTGCCATTACCTGATGAAAAGGAGATTGAGAAGATGGTATTGGAGAAGAAGAAGATGGAACTGTTGAGTAAGTATGCGAGTGATATGCTTTTAGAGGAACAGAGTGAAGCCAAATCCATGCTCAATATACAAAGATAACTCCCAAAATCGTCATAGATTTGTTTAATCTATTTTCCTTACTCAAATTCTCCATGCCTATACTATGCACCACACACAGCTTCTGTGGTCAAATGGCGAAGTCATATTCTGGAATCAAGCAGAGTGATGCAAAAATTGAGGTATATGAGGAACTACGTTGTAATGTTTGTTCAGATCTCTCTATATTTTTGGTTTAAACATCTCTTAGAGATACAATCAACGTTGATAACTGATGTAAAACCTTAGATAATAGGATGTTTGAATTTTCCAAACTGATGTAACTTTGAGAGATGTTTTGGGAGCCGCCCTCTCAAGAGTTGATTTACATTTAGATAAAGACACATATTTAGGCTCTTTCCATAAGGTTCTTTGTATTTCCATTTTGCTTCTTTCGGGTTTAGAAACAAGTTTTCTCCAAGCAGAATGTTGACTTCCCATTCTATTACTTTAGGTGCATGAAACGTCTAGCGAAAACATACAACCATATAACAACGAATGCT

Coding sequence (CDS)

ATGGCTCGAAATGAGGAGAAAGCACAGTCTATGCTGAATCGTTTTATAGCCTTAAAAGCTGAGGAGAAGAAGAAACCCAAGGAACGTCGACCCTACCTTGCCTCTGAATGTCGTGACCTTGCAGAAGCTGACAAATGGCGTCAACAGATCATGCGTGAGATTGGTCGCAAGGTTGCTGAGATCCAAAACGAAGGCCTTGGTGAGCACCGTTTGCGTGATCTCAATGATGAGATTAACAAGCTCATTAGAGAGAAGTCACATTGGGAGCGTCGTATAATTGAGCTTGGTGGCCCCAATTATACCAAACACTCGGCCAAAATGACTGATTTAGATGGGAATATTGTTGATGTACCAAACCCCAGTGGTCGTGGTCCTGGATATCGGTATTTTGGTGCTGCAAAGAAGTTACCAGGTGTTAGGGAATTGTTTGAGAAGCCACCTGAACTGCGGAAGCGCAGGACTCGTTATGACATATACAAGAGGATCGACGCAAGCTACTATGGCTACAGGGACGACGAGGATGGGGTTTTGGAAAGAGTGGAAGGTCCAAGTGAAGAGCGAATGAGAGCTGAGGCAGTTGAAGAGTGGCAAAGAATGGAAGAGATTAGGAAAGAAGCAAAGAGGGCAGTAAAGAGCGGGGAGGTAGCCAGCGTGGTGAATGTTGCAGCAAAGGAGGTACTGTTTGAAGAAGAGGAAGAGGTTGTTGAAGAAGAGAGGAAAAGAGAGAGGGAAATGAAGGAGAATTTGGACAAGGAGAGGGAATTTGTGGTGCATGTGCCATTACCTGATGAAAAGGAGATTGAGAAGATGGTATTGGAGAAGAAGAAGATGGAACTGTTGAGTAAGTATGCGAGTGATATGCTTTTAGAGGAACAGAGTGAAGCCAAATCCATGCTCAATATACAAAGATAA

Protein sequence

MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLNIQR*
Homology
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BLAST of CsGy7G018320 vs. ExPASy Swiss-Prot
Match: Q69ZQ2 (Pre-mRNA-splicing factor ISY1 homolog OS=Mus musculus OX=10090 GN=Isy1 PE=1 SV=2)

HSP 1 Score: 246.9 bits (629), Expect = 2.9e-64
Identity = 151/299 (50.50%), Postives = 198/299 (66.22%), Query Frame = 0 

Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARN EKA + L RF   + EE  K KERRP+LASEC +L +A+KWR+QI+ EI +KVA+
Sbjct: 1   MARNAEKAMTALARFRQAQLEE-GKVKERRPFLASECTELPKAEKWRRQIIGEISKKVAQ 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQN GLGE R+RDLNDEINKL+REK HWE RI ELGGP+Y K   KM D +G  V    P
Sbjct: 61  IQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEV----P 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
             R  GY+YFGAAK LPGVRELFEK P    R+TR ++ K ID  YYGY D++DGV+  +
Sbjct: 121 GNR--GYKYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPL 180

Query: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           E   E+++RAE VE+W+   E R       +  E    +N+ A  V  EE +E   +E+ 
Sbjct: 181 EQEYEKKLRAELVEKWKAEREARLARGEKEEEEEEEEEINIYA--VTEEESDEEGNQEKA 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSML 300
            E       D +++F+ HVP+P ++EIE+ ++ +KKMELL KYAS+ L  +  EAK +L
Sbjct: 241 GE-------DGQQKFIAHVPVPSQQEIEEALVRRKKMELLQKYASETLQAQSEEAKRLL 283

[Close]
BLAST of CsGy7G018320 vs. ExPASy Swiss-Prot
Match: Q6AYB3 (Pre-mRNA-splicing factor ISY1 homolog OS=Rattus norvegicus OX=10116 GN=Isy1 PE=2 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 2.9e-64
Identity = 153/299 (51.17%), Postives = 200/299 (66.89%), Query Frame = 0 

Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARN EKA + L RF   + EE  K KERRP+LASEC +L +A+KWR+QI+ EI +KVA+
Sbjct: 1   MARNAEKAMTALARFRQAQLEE-GKVKERRPFLASECTELPKAEKWRRQIIGEISKKVAQ 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQN GLGE R+RDLNDEINKL+REK HWE RI ELGGP+Y K   KM D +G  V    P
Sbjct: 61  IQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEV----P 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
             R  GY+YFGAAK LPGVRELFEK P    R+TR ++ K ID  YYGY D++DGV+  +
Sbjct: 121 GNR--GYKYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPL 180

Query: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           E   E+R+RAE VE+W+   E R  A+   +  E    +N+ A  V  EE +E   +E+ 
Sbjct: 181 EQEYEKRLRAELVEKWKAEREARL-ARGEKEEEEEEEEINIYA--VTEEESDEEGSQEKA 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSML 300
            E       D +++F+ HVP+P ++EIE+ ++ +KKMELL KYAS+ L  +  EAK +L
Sbjct: 241 GE-------DGQQKFIAHVPVPSQQEIEEALVRRKKMELLQKYASETLQAQSEEAKRLL 282

[Close]
BLAST of CsGy7G018320 vs. ExPASy Swiss-Prot
Match: Q9ULR0 (Pre-mRNA-splicing factor ISY1 homolog OS=Homo sapiens OX=9606 GN=ISY1 PE=1 SV=3)

HSP 1 Score: 244.6 bits (623), Expect = 1.5e-63
Identity = 156/305 (51.15%), Postives = 200/305 (65.57%), Query Frame = 0 

Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARN EKA + L RF   + EE  K KERRP+LASEC +L +A+KWR+QI+ EI +KVA+
Sbjct: 1   MARNAEKAMTALARFRQAQLEE-GKVKERRPFLASECTELPKAEKWRRQIIGEISKKVAQ 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQN GLGE R+RDLNDEINKL+REK HWE RI ELGGP+Y K   KM D +G  V    P
Sbjct: 61  IQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEV----P 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
             R  GY+YFGAAK LPGVRELFEK P    R+TR ++ K ID  YYGY D++DGV+  +
Sbjct: 121 GNR--GYKYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPL 180

Query: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEE-----VV 240
           E   E+++RAE VE+W+   E R      +  GE         KE   EEEEE     V 
Sbjct: 181 EQEYEKKLRAELVEKWKAEREAR------LARGE---------KEEEEEEEEEINIYAVT 240

Query: 241 EEERKRE-REMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSE 300
           EEE   E  + K   D +++F+ HVP+P ++EIE+ ++ +KKMELL KYAS+ L  +  E
Sbjct: 241 EEESDEEGSQEKGGDDSQQKFIAHVPVPSQQEIEEALVRRKKMELLQKYASETLQAQSEE 283

[Close]
BLAST of CsGy7G018320 vs. ExPASy Swiss-Prot
Match: Q54N41 (Pre-mRNA-splicing factor ISY1 homolog OS=Dictyostelium discoideum OX=44689 GN=isy1 PE=3 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 2.8e-54
Identity = 133/306 (43.46%), Postives = 187/306 (61.11%), Query Frame = 0 

Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKA+SMLNR++ LK  E K+ +ERRPYL++EC  L +A+KWR+QI++EI R + E
Sbjct: 1   MARNEEKAKSMLNRYLQLKGTESKQ-EERRPYLSNECDSLVDAEKWRRQILKEITRGITE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQN  L E+++RDLND INKL+REK HWERRI++LGGPN+   + K+ D DG       P
Sbjct: 61  IQNSALDEYKIRDLNDHINKLVREKGHWERRILQLGGPNHRALAPKLFDADGK-----EP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPEL---RKRRTRYDIYKRIDASYYGYRDDEDGVL 180
            G G  YRY+G AK LPGV ELFEKP +     +++TR D+YK ID+ YYGYRDDEDG L
Sbjct: 121 LGTGT-YRYYGEAKNLPGVAELFEKPEQTLHGTEKKTRQDLYKYIDSDYYGYRDDEDGKL 180

Query: 181 ERVEGPSEERMRAEAVEEWQRMEEIRKEA------KRAVKSGEVASVVNVAAKEVLFEEE 240
           E +E   E+    + VE W++ +  + +          +K+    +       E+  +++
Sbjct: 181 EEIEREYEKYAIEQNVENWKKQQRDKFQINTNRFNNNNIKNSIAINKNTENKDEINIDDD 240

Query: 241 EEVVEEERKRER------EMKENLDKERE-----FVVHVPLPDEKEIEKMVLEKKKMELL 287
           EE  +             E  EN+ +E E     F  +V LP   +IE ++LEK+K EL 
Sbjct: 241 EENDDNNNNNNNNNTENTENIENIQEEEEKEDIKFKSYVSLPSTSQIETILLEKRKEELR 299

[Close]
BLAST of CsGy7G018320 vs. ExPASy Swiss-Prot
Match: Q20716 (Protein isy-1 OS=Caenorhabditis elegans OX=6239 GN=isy-1 PE=1 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.0e-45
Identity = 121/284 (42.61%), Postives = 177/284 (62.32%), Query Frame = 0 

Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARN EKA + L R+  +K EE++ P  RRP+   +CR+L++A+++R++I+R+  +K+  
Sbjct: 1   MARNAEKAMTALARWRRMKEEEERGPIARRPHDVKDCRNLSDAERFRREIVRDASKKITA 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQN GLGE +LRDLNDE+N+LI+ K  WE+RI ELGG +Y K++ K  D  G        
Sbjct: 61  IQNPGLGEFKLRDLNDEVNRLIKLKHAWEQRIRELGGTDYRKYAQKELDAIG------RE 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPE-LRKRRTRYDIYKRIDASYYGYRDDEDGVLER 180
           +G   GY+YFGAAK LPGVRELFEK  E   +RR R D+ + IDA Y+GY DDEDG L  
Sbjct: 121 TGNSRGYKYFGAAKDLPGVRELFEKSTEGEEQRRHRADLLRNIDAHYFGYLDDEDGRLIP 180

Query: 181 VEGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEER 240
           +E   EE+           +E I KE   A K  +     + AA E +++ EE+  +++ 
Sbjct: 181 LEKLIEEK----------NIERINKEF--AEKQAQKQQTASDAAPENIYKVEED-DDDDL 240

Query: 241 KREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKY 284
           + +       D     + HV LP +++IE+M+LE+KK EL++KY
Sbjct: 241 ETQESTVIGEDGRPMTIRHVLLPTQQDIEEMLLEQKKQELMAKY 265

[Close]
BLAST of CsGy7G018320 vs. NCBI nr
Match: XP_011659495.1 (pre-mRNA-splicing factor ISY1 homolog [Cucumis sativus] >KGN45211.1 hypothetical protein Csa_016162 [Cucumis sativus])

HSP 1 Score: 566 bits (1460), Expect = 5.20e-203
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0 

Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK
Sbjct: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]
BLAST of CsGy7G018320 vs. NCBI nr
Match: XP_008461190.1 (PREDICTED: pre-mRNA-splicing factor ISY1 homolog [Cucumis melo] >XP_038906088.1 pre-mRNA-splicing factor ISY1 homolog [Benincasa hispida] >XP_038906089.1 pre-mRNA-splicing factor ISY1 homolog [Benincasa hispida] >KAA0058702.1 pre-mRNA-splicing factor ISY1-like protein [Cucumis melo var. makuwa] >TYK10508.1 pre-mRNA-splicing factor ISY1-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 565 bits (1456), Expect = 2.12e-202
Identity = 302/303 (99.67%), Postives = 303/303 (100.00%), Query Frame = 0 

Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDL+GNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLEGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK
Sbjct: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]
BLAST of CsGy7G018320 vs. NCBI nr
Match: XP_022152151.1 (pre-mRNA-splicing factor ISY1 homolog [Momordica charantia])

HSP 1 Score: 562 bits (1449), Expect = 2.47e-201
Identity = 299/303 (98.68%), Postives = 302/303 (99.67%), Query Frame = 0 

Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRI+ELGGPNYTKHSAKMTDLDGNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEERMR EAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK
Sbjct: 181 EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLL+EQSEAK+MLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]
BLAST of CsGy7G018320 vs. NCBI nr
Match: XP_023004198.1 (pre-mRNA-splicing factor ISY1 homolog [Cucurbita maxima] >XP_023514817.1 pre-mRNA-splicing factor ISY1 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 558 bits (1439), Expect = 8.25e-200
Identity = 297/303 (98.02%), Postives = 303/303 (100.00%), Query Frame = 0 

Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRI+ELGGPNYTK+SAKMTDL+GNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKYSAKMTDLEGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEER+RAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEER+
Sbjct: 181 EGPSEERIRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERR 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAK+MLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKTMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]
BLAST of CsGy7G018320 vs. NCBI nr
Match: XP_022960451.1 (pre-mRNA-splicing factor ISY1 homolog [Cucurbita moschata] >KAG6593361.1 Pre-mRNA-splicing factor ISY1-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025707.1 Pre-mRNA-splicing factor ISY1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 557 bits (1435), Expect = 3.36e-199
Identity = 296/303 (97.69%), Postives = 303/303 (100.00%), Query Frame = 0 

Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRI+ELGGPNY+K+SAKMTDL+GNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYSKYSAKMTDLEGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEER+RAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEER+
Sbjct: 181 EGPSEERIRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERR 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAK+MLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKTMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]
BLAST of CsGy7G018320 vs. ExPASy TrEMBL
Match: A0A0A0KBN6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G431430 PE=3 SV=1)

HSP 1 Score: 566 bits (1460), Expect = 2.52e-203
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0 

Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK
Sbjct: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]
BLAST of CsGy7G018320 vs. ExPASy TrEMBL
Match: A0A5D3CH05 (Pre-mRNA-splicing factor ISY1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001580 PE=3 SV=1)

HSP 1 Score: 565 bits (1456), Expect = 1.02e-202
Identity = 302/303 (99.67%), Postives = 303/303 (100.00%), Query Frame = 0 

Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDL+GNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLEGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK
Sbjct: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]
BLAST of CsGy7G018320 vs. ExPASy TrEMBL
Match: A0A1S3CE48 (pre-mRNA-splicing factor ISY1 homolog OS=Cucumis melo OX=3656 GN=LOC103499848 PE=3 SV=1)

HSP 1 Score: 565 bits (1456), Expect = 1.02e-202
Identity = 302/303 (99.67%), Postives = 303/303 (100.00%), Query Frame = 0 

Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDL+GNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLEGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK
Sbjct: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]
BLAST of CsGy7G018320 vs. ExPASy TrEMBL
Match: A0A6J1DGR8 (pre-mRNA-splicing factor ISY1 homolog OS=Momordica charantia OX=3673 GN=LOC111019937 PE=3 SV=1)

HSP 1 Score: 562 bits (1449), Expect = 1.19e-201
Identity = 299/303 (98.68%), Postives = 302/303 (99.67%), Query Frame = 0 

Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRI+ELGGPNYTKHSAKMTDLDGNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKHSAKMTDLDGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEERMR EAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK
Sbjct: 181 EGPSEERMRTEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLL+EQSEAK+MLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLDEQSEAKTMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]
BLAST of CsGy7G018320 vs. ExPASy TrEMBL
Match: A0A6J1KYR8 (pre-mRNA-splicing factor ISY1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111497599 PE=3 SV=1)

HSP 1 Score: 558 bits (1439), Expect = 3.99e-200
Identity = 297/303 (98.02%), Postives = 303/303 (100.00%), Query Frame = 0 

Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE
Sbjct: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKLIREKSHWERRI+ELGGPNYTK+SAKMTDL+GNIVDVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIVELGGPNYTKYSAKMTDLEGNIVDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180

Query: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           EGPSEER+RAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEER+
Sbjct: 181 EGPSEERIRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERR 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300
           REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAK+MLN
Sbjct: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKTMLN 300

Query: 301 IQR 303
           IQR
Sbjct: 301 IQR 303

[Close]
BLAST of CsGy7G018320 vs. TAIR 10
Match: AT3G18790.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Isy1-like splicing (InterPro:IPR009360); Has 1147 Blast hits to 965 proteins in 236 species: Archae - 12; Bacteria - 13; Metazoa - 351; Fungi - 230; Plants - 49; Viruses - 9; Other Eukaryotes - 483 (source: NCBI BLink). )

HSP 1 Score: 460.3 bits (1183), Expect = 1.2e-129
Identity = 243/303 (80.20%), Postives = 270/303 (89.11%), Query Frame = 0 

Query: 1   MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAE 60
           MARNEEKAQSMLNRFI  K  EKKKPKERRPYLASECRDLAEADKWRQQI+REIG KVAE
Sbjct: 1   MARNEEKAQSMLNRFITQKESEKKKPKERRPYLASECRDLAEADKWRQQILREIGSKVAE 60

Query: 61  IQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDLDGNIVDVPNP 120
           IQNEGLGEHRLRDLNDEINKL+RE+ HWERRI+ELGG NY+KHSAKMTDL+GNI+DVPNP
Sbjct: 61  IQNEGLGEHRLRDLNDEINKLLRERYHWERRIVELGGHNYSKHSAKMTDLEGNIIDVPNP 120

Query: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERV 180
           SGRGPGYRYFGAAKKLPGVRELFEKPPELRKR+TRYDIYKRIDASYYGYRDDEDG+LE++
Sbjct: 121 SGRGPGYRYFGAAKKLPGVRELFEKPPELRKRKTRYDIYKRIDASYYGYRDDEDGILEKL 180

Query: 181 EGPSEERMRAEAVEEWQRMEEIRKEAKRAVKSGEVASVVNVAAKEVLFEEEEEVVEEERK 240
           E  SE  MR  +VEEW+R++E+RKEA++             AA+EVLFEEEE+VVEEER 
Sbjct: 181 ERKSEGGMRKRSVEEWRRLDEVRKEARKGASEVVSVGAAAAAAREVLFEEEEDVVEEER- 240

Query: 241 REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN 300
            ERE +E  +KEREFVVHVPLPDEKEIEKMVLEKKKM+LLSKYAS+ L+E+Q+EAKSMLN
Sbjct: 241 MEREKEE--EKEREFVVHVPLPDEKEIEKMVLEKKKMDLLSKYASEDLVEQQTEAKSMLN 300

Query: 301 IQR 304
           I R
Sbjct: 301 IHR 300

The following BLAST results are available for this feature:
BLAST of CsGy7G018320 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q69ZQ22.9e-6450.50Pre-mRNA-splicing factor ISY1 homolog OS=Mus musculus OX=10090 GN=Isy1 PE=1 SV=2[more]
Q6AYB32.9e-6451.17Pre-mRNA-splicing factor ISY1 homolog OS=Rattus norvegicus OX=10116 GN=Isy1 PE=2... [more]
Q9ULR01.5e-6351.15Pre-mRNA-splicing factor ISY1 homolog OS=Homo sapiens OX=9606 GN=ISY1 PE=1 SV=3[more]
Q54N412.8e-5443.46Pre-mRNA-splicing factor ISY1 homolog OS=Dictyostelium discoideum OX=44689 GN=is... [more]
Q207164.0e-4542.61Protein isy-1 OS=Caenorhabditis elegans OX=6239 GN=isy-1 PE=1 SV=1[more]
BLAST of CsGy7G018320 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_011659495.15.20e-203100.00pre-mRNA-splicing factor ISY1 homolog [Cucumis sativus] >KGN45211.1 hypothetical... [more]
XP_008461190.12.12e-20299.67PREDICTED: pre-mRNA-splicing factor ISY1 homolog [Cucumis melo] >XP_038906088.1 ... [more]
XP_022152151.12.47e-20198.68pre-mRNA-splicing factor ISY1 homolog [Momordica charantia][more]
XP_023004198.18.25e-20098.02pre-mRNA-splicing factor ISY1 homolog [Cucurbita maxima] >XP_023514817.1 pre-mRN... [more]
XP_022960451.13.36e-19997.69pre-mRNA-splicing factor ISY1 homolog [Cucurbita moschata] >KAG6593361.1 Pre-mRN... [more]
BLAST of CsGy7G018320 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KBN62.52e-203100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G431430 PE=3 SV=1[more]
A0A5D3CH051.02e-20299.67Pre-mRNA-splicing factor ISY1-like protein OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3CE481.02e-20299.67pre-mRNA-splicing factor ISY1 homolog OS=Cucumis melo OX=3656 GN=LOC103499848 PE... [more]
A0A6J1DGR81.19e-20198.68pre-mRNA-splicing factor ISY1 homolog OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A6J1KYR83.99e-20098.02pre-mRNA-splicing factor ISY1 homolog OS=Cucurbita maxima OX=3661 GN=LOC11149759... [more]
BLAST of CsGy7G018320 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G18790.11.2e-12980.20FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 227..254
NoneNo IPR availableCOILSCoilCoilcoord: 71..91
IPR009360Pre-mRNA-splicing factor Isy1PFAMPF06246Isy1coord: 1..282
e-value: 3.8E-90
score: 301.9
IPR009360Pre-mRNA-splicing factor Isy1PANTHERPTHR13021PRE-MRNA-SPLICING FACTOR ISY1coord: 1..300
IPR029012Helix hairpin bin domain superfamilyGENE3D1.10.287.660Helix hairpin bincoord: 39..96
e-value: 3.2E-29
score: 102.5
IPR037200Pre-mRNA-splicing factor Isy1 superfamilySUPERFAMILY140102ISY1 domain-likecoord: 27..102

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy7G018320.1CsGy7G018320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000350 generation of catalytic spliceosome for second transesterification step
biological_process GO:0000389 mRNA 3'-splice site recognition
cellular_component GO:0071013 catalytic step 2 spliceosome
cellular_component GO:0071014 post-mRNA release spliceosomal complex
cellular_component GO:0071020 post-spliceosomal complex
cellular_component GO:0000974 Prp19 complex