CsGy7G013035 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy7G013035
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionPectinesterase
LocationGy14Chr7: 16863882 .. 16869437 (-)
RNA-Seq ExpressionCsGy7G013035
SyntenyCsGy7G013035
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCTTTCTTCACAACTCTTCTCATTTCCAAACTATGGAAAACAATAAACTTTCACTCGCTCTCTTAGCTATTTTACTCCTCACAAATGCTATTGTTGTTAGAGCTAATTCTAATGAAAATAGTGTCATAGAAACCTCATGTGCCATTACTCTTTATCCTCAACTCTGTCATTCAACAATATCCTCCATCGTTGGAACGTCTAATTTGTTGAGTTTGAAAGATATTTTTGAAGTGTCATTGAGTGTTGCTATGGATGCAGCTAAGCATAACAATAAAAACATAAAAAAACTTATGGTGTCCACTAACAATGTTAGTAAACGAGACAAGATTGGTCTTCATGATTGTGTTGAAACAACTGACAGGACCATTTATGAGCTTGGGAAAGCTATAGAGGTCTTTCGTGAGTATCCGAACAAGAGATCTCTTACTTTGTACGCCGATGACCTCAAAACCTTCCTCAGTTCTGCCATTACTAATCAGGTTTGCAAATTCGTCTACCATCTTGATTATCTTGCTCATTTTGAAGTTAATAATGTATTGTGTACGTATACCTTTTTATTTTTGGTTCAGGTGACTTGTTTGGATGGATTATCACATGACAAAACAGAGAAAAGGGTTTTGAGACTTATTGAGAACGAACATATTCATGTGACAAAGTTGTGTAGTAATGCATTGGCTTTGGTTCAAAAATTGACAACAGACATTGCCATAACAGATGAGAAAAGCTTGGTGGTCCATGATTTCCCTTATAAGATAACTTCAATACCCTCCCAAATGGATGACCCCAAAATTGTACTCTTTTCCAACCAGGTATAATAGTGTTTTCAACTTTATTTCAAACCCTTTTATTTTGCTTTTATAAATATTTTTACTCCTACTCGTGCATTTTCAATCCTTTTAACTTTCTCAACAGAATATCCTGGTGCAGAAGCAGAACTACTGATTCCAATCACTTTTTTAGCTATGTTGTTTGGGTTCACAACTATAGTTTGTTAACTTAATTACTTACCATGAACATCTGTTGTTTTCATAAATTTATTGTGTTTTCATAACTCTACCGATCATTTTCTTCTAGTATCTTGTTAAAAAAAATTACCTCTAAATCATCCTTAAAAGGTTTAATAAGTCTTTAAACTATCCATTAATTTTGTGTATAAGTTGGTTAACTTTCAAGTTTACATATAATATAGGGATTTTGAAATTTTCAAAATTTCCAAAAATTAGTTGTTCTTCTTAAATATAATTTTCTATATTGGAACCCTATAGTATAAAAATTAATGATATATCAAATAAAATTTTAAAGTATACACTTTTTCATAATTTAAAAGAAATTTTTTTATAAATATAACCAAACATCCAAAACTCTACAAAGGTGGCTATTGTTTTTTAAATATTCCATGTTTATACTTTCTTTTCATCTTCTCCAATTTTTTTTCCTTTCCATTATATTTTTATTTCTTATGCAATTTTTTTCCTCCTATGTTTATTTCAAACTGTTATTTGGTCGTTCAAGATCTATGTCAAATATAAAATATAATGAAAAAATCGTTAGTATATTAGGTATAACCAAATCTAAACGATCATGTATCATATAGTACCAAAATTATCTTTAAAAAAAATTGTATAGATTTGGCTAGCTACCCAAATTTAAACAATCAAATCTAAACAATCATGTACCAAATTTAAACGACCGTGTATGAAAAATCTTTGAAAAAGACTTGTTTACATTTGGTGAAATCTAAATGATCGTGTACTAAAAAATCTTGAAAAGAACTCATTTACATTTGGTGAAATCTAAATGACCGTGTACTAAATTACAATAGCTAAATCTAACAAATCTAAACGATCGTGTACCAAAGAATCTTGAAAAAAATTATTTAAATTTGGTCAAAATCTAAACAATCAAATCTAAACGATCATATATCAAAGAAACAACCAATTCTAAACGATAGTGTACAAAAAAATATTGAAAACGAATATTTAGATTTGGAACGATTGTTTACCCAATATACTGCCAATTAACCACGCAGACATTTTTTTTGTATTTTTTATTATTGGTTTGTGAGTTTTTTCCATTTTCGAAATTGTTCTATATACACCGTAATTATTTTGGTCTTTTGTTATATTTTTTAAAAGATTTCTAATATTTTAAAATGCTCATTTAGTTATCTAAGAACTGGAGTACCAGGACTAAAGAATTAATCTTGCCAAAAATATAATAAGAATACAATTTTTCTAACTTGAAATATGAAAGCAAACTCTTTTAGTTATAATTAATGTAAATAAGTGGAGAACTACAAAATTAATTTTGCCAAAAATCAGTATTCTATATTTGTAGAAATATGACAGCGAAAATTTGTAGTAATAACTAGTGTACTTTGATTCCTATCTTGGCTCTAAATAGGCTTTGTAGTGAACAAATCCAACACGTTTCTAGATCCAATCATAGACCATAACTTCTTCCATGTAGTTTAGTTTTTTTCAATAAGATTCAACCTCTGATCTCAAACGAGTAGGTGTGAGATATATTGATCTATTACTAGTGCAGAAAACAAAAAAGAAAGTATTATATTGATATTATTATTTAAGTTCATGTTGATATCAACAATATGTAGAATATAATTAACAGGAGGAAGATGAGAATAGAAGAAGGGAGGAGTTAGAAGATGGAATAAAGTGGCCAAAGTGGATGTCAATCGAAGACCAGAAGCTTTTGGAATCATCATCAGAAGCAGCAGCGGAGGCTGTGGTGGCGGCGGATGGAAGTGGAAATTATAAAACGGTGGCGGAGGCGGTGGCGGCAGCTCCGAGTAAGAACAGCAAAAGATATATAATAAAAATAAAGGCTGGAGAGTACTGGGAAAATGTGGATGTACCAAGTAGTAAAAGGAATATTATGTTTTGGGGAGATGGAAGATCCAACACCAAAATCATTTCTAATAGAAGTCATGGAACTGGCTGGTCAACCTTCAGATCCGCCACACTTGGTAACACAAACTAAACCACAATACTCTTTTCTTTTCTTTTCTTTTTGTTTGAATTGTAGCTCCATTTTCTTCTTTTTAATTTAAGTGTTTTCTCCAAAAATAAATATAATAAAATAAACTAAAATTTCTTTTTGTTGATTTTAAGATATATATTTTACGTATGTTCTCTAAAAAAAATGTTTTATTTTCTTAATTTCGTTGCATTATATTTGGACATTTATAAGGAATTATAGATCGCAAAAATGCAACTATTTGTTTTGGATAATTGTAATGGATGACTATTTGAGGAATAATAATTAAGAATATAGCAATATTTTAAAAAAATTGTAAATAAAGCAAAACTATCGTTGTTAGACTTGTATCATTGATAGAATTTGACCAATTTCGTTATATTAATAAATTTTTTAAAATTTTGTTATATATTTAATTATTTCTAATCTAATTGCTAAATTTGCAACTATCTCATTTGTTTTTCCTTTTTTTTCATATTTTTTTTGCCGAAGTTGGTCATTCAAAACTTAAAATTGGTAAAGGAAAAACTTGAATGATTTAGGAAAAATTATTAGTTAAACCACTTTCTCTCAAAAACCAATTTTATTGGCCCATGATAACACTTAAAAGTTTAACTTGTTTAAAAAATGAGATAGTTGCAAATTTAGCAATTAGATTTAAAATAATTAAATATATAGCAACATTTAAACAAAATATTCAAATATAGCAAAATTTGTCAAATTCTATCAATGATACAAGTCTATCACCGATAAATCATATGGTAAATATTGGTCTATCACTGATAGACCATACAAGTCTATCAGCAATAGTTTTGCTATATTTGTAAAAAAATTTAAATGTTGTTATATCCTTAATTATTATTCCACAAATTGTCATCCATTACAACTACCCTTAAAAGTTATGTGTTTTGAGTCATTTAAAGTCTAACAGATACGATTTAAACAGTCCGAAAATCACTTTCAAACAAACGAAAAAGTATTTGCTAATATATGTCAAAATAAGTATATAGTTCAATTGACATGTGCTCAACCTCAAAGTTGTTAGAGTTTTGGTTCTCCAACTCATGCATTGTCCCAATAACTCTAGAAAAACAAATGGAAAATTGTCAAAAATAGCAAACTTGACAAAATTTTTACAACATATAACAATTTTTTTTAGATTTTATCAATAATAAACATTAATAGTCACTGATATGCTTCTATTAGTGGCATCGTGACTATTATTGATAGAATCCAAAATTTTGCTATAGCTAATAAATATTTTAATTTATTTTACTATTTTTAAAAATACTCCCAAACAAATTACTTTCGAACCTAACAATTTTTTTTCATATTCTCAAAACCAATTCCAAACAAACTTTTGATTATTATTGATCAAATGAAAATTGCTTCTATAAAAATCAAATTAAAATAAGAACAAAGAAACAATAAAATTGATTTTAAATAATTAAAAGCATATTTCATATCAGTATCCATATGATGATTTTATATATGTTTGGATTGACATTTTAGTACTTATAAACACTAGCTTATTTAACCACTTCAACGATTATAAGTGTGTTTGGATTAACTTTGCAATGGTTTATAAATACTTCTAATTATTTATCAAACCATTGGTCATTACATTAAAAAAAATACCCACATTTTAATAACAATGTGCTGTTTTTCTGTGACAGCTGCGGTGGGGGATGGTTTTTTAGCTCGAGACATAACCTTTCAAAACAAAGCCGGAGCAGCCAACGGCCAAGCTGTAGCTCTCCGTGTTGGCTCAGATCACTCGGCCTTCTACCGATGTAGCATGCTTGCCTACCAAGACACTCTCTACGTTCACTCGAACCGTCAATTCTTCGTCAACTGCATTGTAGCAGGCACAGTCGATTTCATCTTTGGAAATGCAGCAGCCGTATTCCAAAACTCTGACATCACCCCTCGCAAACCAGGGCCTAGTCAAAGGAACATGGTCACAGCACAATCAAGAACCGACATTAATCAAAACACGGGTATCGTCATCCAAAAGTGTAGGATAAAAGCAACGTCAGACTTGGAGCCTGTGATAGAAGAATTCCCAAGCTTTCTCGGGAGACCATGGGAAGAATATGCTAGAGTTGTGGTGATGCAAACGACAATAAGCAATGTGATAGACAAAGAGGGGTGGTCCACATGGAATGGCCAAAGAAAGTCACCATACTATGCGGAATATGATAATAATGGAGCTGGAGCCGATATTTCTGGTAGAGTGCCATGGTCTCTTGTTATTGATGAGGCTCAAGCTAAGACTTTTACGGCTGGACCATTTATTGGTGGAGCTGATTGGCTTTCATCTACTGGCTTTCCCTATCAGCTTAGTCTCTATAAATGATCTAATGCGCCTAATTAATTAGCTTGCAACTCTTGCTTTTGGTTTGGTTGGTCAAAACGATGTATTAGAGTTCTAAGCTATAGTAAATGTCGAATGCTTTAGTTAAATAAAATAAAGTCACTTAATTAAAATCTTTTGTTTTTGTGTCTTGGTAAGTTCGTTTTAATTTTAGCTTTTGATGAACACTCTTCAAGAAAC

mRNA sequence

TTTCTTTCTTCACAACTCTTCTCATTTCCAAACTATGGAAAACAATAAACTTTCACTCGCTCTCTTAGCTATTTTACTCCTCACAAATGCTATTGTTGTTAGAGCTAATTCTAATGAAAATAGTGTCATAGAAACCTCATGTGCCATTACTCTTTATCCTCAACTCTGTCATTCAACAATATCCTCCATCGTTGGAACGTCTAATTTGTTGAGTTTGAAAGATATTTTTGAAGTGTCATTGAGTGTTGCTATGGATGCAGCTAAGCATAACAATAAAAACATAAAAAAACTTATGGTGTCCACTAACAATGTTAGTAAACGAGACAAGATTGGTCTTCATGATTGTGTTGAAACAACTGACAGGACCATTTATGAGCTTGGGAAAGCTATAGAGGTCTTTCGTGAGTATCCGAACAAGAGATCTCTTACTTTGTACGCCGATGACCTCAAAACCTTCCTCAGTTCTGCCATTACTAATCAGGTGACTTGTTTGGATGGATTATCACATGACAAAACAGAGAAAAGGGTTTTGAGACTTATTGAGAACGAACATATTCATGTGACAAAGTTGTGTAGTAATGCATTGGCTTTGGTTCAAAAATTGACAACAGACATTGCCATAACAGATGAGAAAAGCTTGGTGGTCCATGATTTCCCTTATAAGATAACTTCAATACCCTCCCAAATGGATGACCCCAAAATTGTACTCTTTTCCAACCAGGAGGAAGATGAGAATAGAAGAAGGGAGGAGTTAGAAGATGGAATAAAGTGGCCAAAGTGGATGTCAATCGAAGACCAGAAGCTTTTGGAATCATCATCAGAAGCAGCAGCGGAGGCTGTGGTGGCGGCGGATGGAAGTGGAAATTATAAAACGGTGGCGGAGGCGGTGGCGGCAGCTCCGAGTAAGAACAGCAAAAGATATATAATAAAAATAAAGGCTGGAGAGTACTGGGAAAATGTGGATGTACCAAGTAGTAAAAGGAATATTATGTTTTGGGGAGATGGAAGATCCAACACCAAAATCATTTCTAATAGAAGTCATGGAACTGGCTGGTCAACCTTCAGATCCGCCACACTTGCTGCGGTGGGGGATGGTTTTTTAGCTCGAGACATAACCTTTCAAAACAAAGCCGGAGCAGCCAACGGCCAAGCTGTAGCTCTCCGTGTTGGCTCAGATCACTCGGCCTTCTACCGATGTAGCATGCTTGCCTACCAAGACACTCTCTACGTTCACTCGAACCGTCAATTCTTCGTCAACTGCATTGTAGCAGGCACAGTCGATTTCATCTTTGGAAATGCAGCAGCCGTATTCCAAAACTCTGACATCACCCCTCGCAAACCAGGGCCTAGTCAAAGGAACATGGTCACAGCACAATCAAGAACCGACATTAATCAAAACACGGGTATCGTCATCCAAAAGTGTAGGATAAAAGCAACGTCAGACTTGGAGCCTGTGATAGAAGAATTCCCAAGCTTTCTCGGGAGACCATGGGAAGAATATGCTAGAGTTGTGGTGATGCAAACGACAATAAGCAATGTGATAGACAAAGAGGGGTGGTCCACATGGAATGGCCAAAGAAAGTCACCATACTATGCGGAATATGATAATAATGGAGCTGGAGCCGATATTTCTGGTAGAGTGCCATGGTCTCTTGTTATTGATGAGGCTCAAGCTAAGACTTTTACGGCTGGACCATTTATTGGTGGAGCTGATTGGCTTTCATCTACTGGCTTTCCCTATCAGCTTAGTCTCTATAAATGATCTAATGCGCCTAATTAATTAGCTTGCAACTCTTGCTTTTGGTTTGGTTGGTCAAAACGATGTATTAGAGTTCTAAGCTATAGTAAATGTCGAATGCTTTAGTTAAATAAAATAAAGTCACTTAATTAAAATCTTTTGTTTTTGTGTCTTGGTAAGTTCGTTTTAATTTTAGCTTTTGATGAACACTCTTCAAGAAAC

Coding sequence (CDS)

ATGGAAAACAATAAACTTTCACTCGCTCTCTTAGCTATTTTACTCCTCACAAATGCTATTGTTGTTAGAGCTAATTCTAATGAAAATAGTGTCATAGAAACCTCATGTGCCATTACTCTTTATCCTCAACTCTGTCATTCAACAATATCCTCCATCGTTGGAACGTCTAATTTGTTGAGTTTGAAAGATATTTTTGAAGTGTCATTGAGTGTTGCTATGGATGCAGCTAAGCATAACAATAAAAACATAAAAAAACTTATGGTGTCCACTAACAATGTTAGTAAACGAGACAAGATTGGTCTTCATGATTGTGTTGAAACAACTGACAGGACCATTTATGAGCTTGGGAAAGCTATAGAGGTCTTTCGTGAGTATCCGAACAAGAGATCTCTTACTTTGTACGCCGATGACCTCAAAACCTTCCTCAGTTCTGCCATTACTAATCAGGTGACTTGTTTGGATGGATTATCACATGACAAAACAGAGAAAAGGGTTTTGAGACTTATTGAGAACGAACATATTCATGTGACAAAGTTGTGTAGTAATGCATTGGCTTTGGTTCAAAAATTGACAACAGACATTGCCATAACAGATGAGAAAAGCTTGGTGGTCCATGATTTCCCTTATAAGATAACTTCAATACCCTCCCAAATGGATGACCCCAAAATTGTACTCTTTTCCAACCAGGAGGAAGATGAGAATAGAAGAAGGGAGGAGTTAGAAGATGGAATAAAGTGGCCAAAGTGGATGTCAATCGAAGACCAGAAGCTTTTGGAATCATCATCAGAAGCAGCAGCGGAGGCTGTGGTGGCGGCGGATGGAAGTGGAAATTATAAAACGGTGGCGGAGGCGGTGGCGGCAGCTCCGAGTAAGAACAGCAAAAGATATATAATAAAAATAAAGGCTGGAGAGTACTGGGAAAATGTGGATGTACCAAGTAGTAAAAGGAATATTATGTTTTGGGGAGATGGAAGATCCAACACCAAAATCATTTCTAATAGAAGTCATGGAACTGGCTGGTCAACCTTCAGATCCGCCACACTTGCTGCGGTGGGGGATGGTTTTTTAGCTCGAGACATAACCTTTCAAAACAAAGCCGGAGCAGCCAACGGCCAAGCTGTAGCTCTCCGTGTTGGCTCAGATCACTCGGCCTTCTACCGATGTAGCATGCTTGCCTACCAAGACACTCTCTACGTTCACTCGAACCGTCAATTCTTCGTCAACTGCATTGTAGCAGGCACAGTCGATTTCATCTTTGGAAATGCAGCAGCCGTATTCCAAAACTCTGACATCACCCCTCGCAAACCAGGGCCTAGTCAAAGGAACATGGTCACAGCACAATCAAGAACCGACATTAATCAAAACACGGGTATCGTCATCCAAAAGTGTAGGATAAAAGCAACGTCAGACTTGGAGCCTGTGATAGAAGAATTCCCAAGCTTTCTCGGGAGACCATGGGAAGAATATGCTAGAGTTGTGGTGATGCAAACGACAATAAGCAATGTGATAGACAAAGAGGGGTGGTCCACATGGAATGGCCAAAGAAAGTCACCATACTATGCGGAATATGATAATAATGGAGCTGGAGCCGATATTTCTGGTAGAGTGCCATGGTCTCTTGTTATTGATGAGGCTCAAGCTAAGACTTTTACGGCTGGACCATTTATTGGTGGAGCTGATTGGCTTTCATCTACTGGCTTTCCCTATCAGCTTAGTCTCTATAAATGA

Protein sequence

MENNKLSLALLAILLLTNAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENEHIHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFRSATLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADISGRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK*
Homology
BLAST of CsGy7G013035 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 2.6e-154
Identity = 304/592 (51.35%), Postives = 393/592 (66.39%), Query Frame = 0

Query: 2   ENNKLSLALLAILLLTNAIV-------VRANSNEN------SVIETSCAITLYPQLCHST 61
           +  KL LAL A LL+  A++        R NS +N      +++++SC+ T YP LC S 
Sbjct: 26  KKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSA 85

Query: 62  ISSIVGTS-NLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVET 121
           I+++   S  + S KD+ E+SL++   A +HN   I+KL+  T N++KR+K+ LHDC+ET
Sbjct: 86  IAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT-NLTKREKVALHDCLET 145

Query: 122 TDRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLR 181
            D T+ EL KA+E   EYPNK+SL+ +ADDLKT +S+A+TNQ TCLDG SHD   K V  
Sbjct: 146 IDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRD 205

Query: 182 LIENEHIHVTKLCSNALALVQKLT-TDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLF 241
            + +  +HV K+CSNALA+++ +T TD+ I                              
Sbjct: 206 ALSDGQVHVEKMCSNALAMIKNMTDTDMMIM----------------------------- 265

Query: 242 SNQEEDENRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVA 301
             +  +  +  EE      WP W+S  D++LL+SSS     AVVAADGSGN+KTVA AVA
Sbjct: 266 --RTSNNRKLTEETSTVDGWPAWLSPGDRRLLQSSS-VTPNAVVAADGSGNFKTVAAAVA 325

Query: 302 AAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFRSA 361
           AAP   +KRYII+IKAG Y ENV+V    +NIMF GDGR+ T I  +R+   G +TF+SA
Sbjct: 326 AAPQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSA 385

Query: 362 TLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFF 421
           T A VG+GFLARDITFQN AG +  QAVALRVG+D SAFY C MLAYQDTLYVHSNRQFF
Sbjct: 386 TAAVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFF 445

Query: 422 VNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIK 481
           VNC++AGTVDFIFGNAAAV QN DI  RKP   Q+NMVTAQ RTD NQNTGIVIQK RI 
Sbjct: 446 VNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIG 505

Query: 482 ATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQ--RKSPYYAEYD 541
           ATSDL+PV   FP++LGRPW+EY+R V+MQ++I+++I   GW  W+G     + +Y E+ 
Sbjct: 506 ATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQ 565

Query: 542 NNGAGADISGRVPWS---LVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 574
           N+GAGA  SGRV W    ++    +A+ FT G FI G+ WL STGFP+ L L
Sbjct: 566 NSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of CsGy7G013035 vs. ExPASy Swiss-Prot
Match: Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 7.6e-154
Identity = 291/562 (51.78%), Postives = 387/562 (68.86%), Query Frame = 0

Query: 18  NAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTS-NLLSLKDIFEVSLSVAMDAA 77
           +A ++  +S+ +++++++C+ TL+P+LC+S I ++   S  + S KD+ E+SL++ + A 
Sbjct: 52  HADIMAISSSAHAIVKSACSNTLHPELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAV 111

Query: 78  KHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIEVFREYPNKRSLTLYAD 137
           + N   +K+L+ +   ++ R+K+ LHDC+ET D T+ EL  A+E    YPNK+SL  + +
Sbjct: 112 RRNYYAVKELIKTRKGLTPREKVALHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVE 171

Query: 138 DLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENEHIHVTKLCSNALALVQKLTTDIAI 197
           DLKT +SSAITNQ TCLDG SHD+ +K+V +++     HV K+CSNALA++  + TD  I
Sbjct: 172 DLKTLISSAITNQETCLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICNM-TDTDI 231

Query: 198 TDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREELEDGIKWPKWMSIEDQK 257
            +E  L          S P+                    R+ +ED  +WP+W+S  D++
Sbjct: 232 ANEMKL----------SAPA------------------NNRKLVEDNGEWPEWLSAGDRR 291

Query: 258 LLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIKIKAGEYWENVDVPSSKR 317
           LL+SS+    + VVAADGSG+YKTV+EAV  AP K+SKRY+I+IKAG Y ENVDVP  K 
Sbjct: 292 LLQSST-VTPDVVVAADGSGDYKTVSEAVRKAPEKSSKRYVIRIKAGVYRENVDVPKKKT 351

Query: 318 NIMFWGDGRSNTKIISNRSHGTGWSTFRSATLAAVGDGFLARDITFQNKAGAANGQAVAL 377
           NIMF GDG+SNT I ++R+   G +TF SAT+  V    LARDITFQN AGA+  QAVAL
Sbjct: 352 NIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAGKVLARDITFQNTAGASKHQAVAL 411

Query: 378 RVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIFGNAAAVFQNSDITPRKP 437
            VGSD SAFYRC MLAYQDTLYVHSNRQFFV C+VAGTVDFIFGN AAVFQ+ DI  R+P
Sbjct: 412 CVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAGTVDFIFGNGAAVFQDCDIHARRP 471

Query: 438 GPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFPSFLGRPWEEYARVVVMQ 497
           G  Q+NMVTAQ RTD NQNTGIVIQKCRI ATSDL PV + FP++LGRPW+EY+R V+MQ
Sbjct: 472 GSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGRPWKEYSRTVIMQ 531

Query: 498 TTISNVIDKEGWSTWNGQ--RKSPYYAEYDNNGAGADISGRVPW---SLVIDEAQAKTFT 557
           ++I++VI   GW  WNG     + +Y EY N GAGA  SGRV W    ++    +A+ +T
Sbjct: 532 SSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAPTSGRVKWKGHKVITSSTEAQAYT 583

Query: 558 AGPFIGGADWLSSTGFPYQLSL 574
            G FI G  WLSSTGFP+ L L
Sbjct: 592 PGRFIAGGSWLSSTGFPFSLGL 583

BLAST of CsGy7G013035 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 3.8e-153
Identity = 302/592 (51.01%), Postives = 391/592 (66.05%), Query Frame = 0

Query: 2   ENNKLSLALLAILLLTNAIV-------VRANSNEN------SVIETSCAITLYPQLCHST 61
           +  KL LAL A LL+  A++        R NS +N      +++++SC+ T YP LC S 
Sbjct: 26  KKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSA 85

Query: 62  ISSIVGTS-NLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVET 121
           I+++   S  + S KD+ E+SL++   A +HN   I+KL+  T N++KR+K+ LHDC+ET
Sbjct: 86  IAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT-NLTKREKVALHDCLET 145

Query: 122 TDRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLR 181
            D T+ EL KA+E   EYPNK+SL+ +ADDLKT +S+A+TNQ TCLDG SHD   K V  
Sbjct: 146 IDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRD 205

Query: 182 LIENEHIHVTKLCSNALALVQKLT-TDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLF 241
            + +  +HV K+CSNALA+++ +T TD+ I                              
Sbjct: 206 ALSDGQVHVEKMCSNALAMIKNMTDTDMMIM----------------------------- 265

Query: 242 SNQEEDENRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVA 301
             +  +  +  EE      WP W+S  D++LL+SSS      VVAADGSGN+KTVA +VA
Sbjct: 266 --RTSNNRKLIEETSTVDGWPAWLSTGDRRLLQSSS-VTPNVVVAADGSGNFKTVAASVA 325

Query: 302 AAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFRSA 361
           AAP   +KRYII+IKAG Y ENV+V    +NIMF GDGR+ T I  +R+   G +TF+SA
Sbjct: 326 AAPQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSA 385

Query: 362 TLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFF 421
           T+A VG+GFLARDITFQN AG +  QAVALRVG+D SAFY C MLAYQDTLYVHSNRQFF
Sbjct: 386 TVAVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFF 445

Query: 422 VNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIK 481
           VNC++AGTVDFIFGNAAAV QN DI  RKP   Q+NMVTAQ R D NQNTGIVIQK RI 
Sbjct: 446 VNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIG 505

Query: 482 ATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQ--RKSPYYAEYD 541
           ATSDL+PV   FP++LGRPW+EY+R V+MQ++I++VI   GW  W+G     + +Y E+ 
Sbjct: 506 ATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQ 565

Query: 542 NNGAGADISGRVPWS---LVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 574
           N GAGA  SGRV W    ++    +A+ FT G FI G+ WL STGFP+ L L
Sbjct: 566 NAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of CsGy7G013035 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 530.0 bits (1364), Expect = 3.3e-149
Identity = 304/594 (51.18%), Postives = 391/594 (65.82%), Query Frame = 0

Query: 2   ENNKLSLALLAILLLTNAIVV-------RANS------NENSVIETSCAITLYPQLCHST 61
           +N KL L   A+ LL  A V        +AN       + ++V+ +SC+ T YP+LC S 
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISA 75

Query: 62  ISSIVGTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETT 121
           + +  G   L S KD+ E S+++ + A +HN   +KKL+     ++ R+K  LHDC+ET 
Sbjct: 76  VVT-AGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETI 135

Query: 122 DRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRL 181
           D T+ EL + +E    YP K++L  +A DLKT +SSAITNQ TCLDG SHD  +K+V + 
Sbjct: 136 DETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKA 195

Query: 182 IENEHIHVTKLCSNALALVQKLT-TDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFS 241
           +    IHV  +CSNALA+++ +T TDIA  ++K+        KITS   ++ +       
Sbjct: 196 LLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKA--------KITSNNRKLKE------E 255

Query: 242 NQEED---ENRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEA 301
           NQE     +     EL D   WP W+S  D++LL+ S    A+A VAADGSG +KTVA A
Sbjct: 256 NQETTVAVDIAGAGEL-DSEGWPTWLSAGDRRLLQGSG-VKADATVAADGSGTFKTVAAA 315

Query: 302 VAAAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFR 361
           VAAAP  ++KRY+I IKAG Y ENV+V   K+NIMF GDGR+ T I  +R+   G +TF 
Sbjct: 316 VAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFH 375

Query: 362 SATLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQ 421
           SAT+AAVG+ FLARDITFQN AG +  QAVALRVGSD SAFY C MLAYQDTLYVHSNRQ
Sbjct: 376 SATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQ 435

Query: 422 FFVNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCR 481
           FFV C++AGTVDFIFGNAA V Q+ DI  R+P   Q+NMVTAQ RTD NQNTGIVIQKCR
Sbjct: 436 FFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCR 495

Query: 482 IKATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNG--QRKSPYYAE 541
           I ATSDL+ V   FP++LGRPW+EY++ V+MQ+ IS+VI  EGWS W G     +  Y E
Sbjct: 496 IGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYRE 555

Query: 542 YDNNGAGADISGRVPW---SLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 574
           Y N GAGA  + RV W    ++   A+A+ +TAG FIGG  WLSSTGFP+ L L
Sbjct: 556 YSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of CsGy7G013035 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 524.6 bits (1350), Expect = 1.4e-147
Identity = 294/598 (49.16%), Postives = 390/598 (65.22%), Query Frame = 0

Query: 2   ENNK---LSLALLAILLLTNAIVVRA---NSNEN-----------SVIETSCAITLYPQL 61
           +NNK   LS A +A+LLL + + + A   N N+N           +++++ C+ TLYP+L
Sbjct: 15  KNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPEL 74

Query: 62  CHSTISSIVGTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDC 121
           C S +++  G   L S K++ E SL++   A KHN   +KKL+     ++ R+   LHDC
Sbjct: 75  CFSAVAA-TGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDC 134

Query: 122 VETTDRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKR 181
           +ET D T+ EL  A+E   +YP ++SL  +ADDLKT +SSAITNQ TCLDG S+D  +++
Sbjct: 135 LETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRK 194

Query: 182 VLRLIENEHIHVTKLCSNALALVQKLT-TDIA---ITDEKSLVVHDFPYKITSIPSQMDD 241
           V + +    +HV  +CSNALA+++ +T TDIA   + D+ S   ++   K+  +   +D 
Sbjct: 195 VRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDLD- 254

Query: 242 PKIVLFSNQEEDENRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKT 301
                                DG  WPKW+S+ D++LL+ S+   A+A VA DGSG++ T
Sbjct: 255 --------------------SDG--WPKWLSVGDRRLLQGST-IKADATVADDGSGDFTT 314

Query: 302 VAEAVAAAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGW 361
           VA AVAAAP K++KR++I IKAG Y ENV+V   K NIMF GDGR  T I  +R+   G 
Sbjct: 315 VAAAVAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGS 374

Query: 362 STFRSATLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVH 421
           +TF SAT+AAVG+ FLARDITFQN AG +  QAVALRVGSD SAFY+C M AYQDTLYVH
Sbjct: 375 TTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVH 434

Query: 422 SNRQFFVNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVI 481
           SNRQFFV C + GTVDFIFGNAAAV Q+ DI  R+P   Q+NMVTAQ R+D NQNTGIVI
Sbjct: 435 SNRQFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVI 494

Query: 482 QKCRIKATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQ--RKSP 541
           Q CRI  TSDL  V   FP++LGRPW+EY+R V+MQ+ IS+VI  EGW  W+G     + 
Sbjct: 495 QNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTL 554

Query: 542 YYAEYDNNGAGADISGRVPW---SLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 574
            Y EY N G GA  + RV W    ++  + +A+ FTAG FIGG  WL+STGFP+ LSL
Sbjct: 555 TYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of CsGy7G013035 vs. NCBI nr
Match: XP_004139620.2 (pectinesterase [Cucumis sativus] >KGN44621.1 hypothetical protein Csa_015743 [Cucumis sativus])

HSP 1 Score: 1124 bits (2907), Expect = 0.0
Identity = 573/575 (99.65%), Postives = 574/575 (99.83%), Query Frame = 0

Query: 1   MENNKLSLALLAILLLTNAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSNLLS 60
           MENNKLSLALLAILLLTNAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSNLLS
Sbjct: 1   MENNKLSLALLAILLLTNAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSNLLS 60

Query: 61  LKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIE 120
           LKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIE
Sbjct: 61  LKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIE 120

Query: 121 VFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENEHIHVTKLC 180
           VFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIEN HIHVTKLC
Sbjct: 121 VFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAHIHVTKLC 180

Query: 181 SNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREEL 240
           SNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREEL
Sbjct: 181 SNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREEL 240

Query: 241 EDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIKI 300
           EDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIKI
Sbjct: 241 EDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIKI 300

Query: 301 KAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFRSATLAAVGDGFLARDI 360
           KAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTF+SATLAAVGDGFLARDI
Sbjct: 301 KAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAVGDGFLARDI 360

Query: 361 TFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIFG 420
           TFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIFG
Sbjct: 361 TFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIFG 420

Query: 421 NAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFPS 480
           NAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFPS
Sbjct: 421 NAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFPS 480

Query: 481 FLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADISGRVPWSL 540
           FLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADISGRVPWSL
Sbjct: 481 FLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADISGRVPWSL 540

Query: 541 VIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK 575
           VIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK
Sbjct: 541 VIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK 575

BLAST of CsGy7G013035 vs. NCBI nr
Match: XP_011659194.1 (pectinesterase [Cucumis sativus] >KGN44619.1 hypothetical protein Csa_015815 [Cucumis sativus])

HSP 1 Score: 957 bits (2475), Expect = 0.0
Identity = 491/582 (84.36%), Postives = 529/582 (90.89%), Query Frame = 0

Query: 1   MENNKLSLALLAILLLTNAIVVRANSNEN-------SVIETSCAITLYPQLCHSTISSIV 60
           MEN KLSLAL A LLLTNAI+VRANSNEN       SVIETSCAITLYPQLCHSTISSI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMNNILSVIETSCAITLYPQLCHSTISSIL 60

Query: 61  GTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIY 120
           GTSNLLSLKDI E+SLSVAMDAAK NN+NIKKL +S N V KRD+I LHDCV+TTDRTIY
Sbjct: 61  GTSNLLSLKDIVELSLSVAMDAAKLNNENIKKLSMSGNIVGKRDRIALHDCVKTTDRTIY 120

Query: 121 ELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENEH 180
           EL KAI+ F EYPNK+SL  YADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIEN H
Sbjct: 121 ELDKAIQDFSEYPNKKSLASYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENTH 180

Query: 181 IHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDE 240
             VTKLCSNALALV+KLTTD+A+TDEKSLVVHDFPYKITSI SQ+DDPKIVLFSNQE+ E
Sbjct: 181 NQVTKLCSNALALVKKLTTDVALTDEKSLVVHDFPYKITSISSQIDDPKIVLFSNQEDGE 240

Query: 241 NRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNS 300
           +RRRE+LE+GIKWP+WMSI+DQ LL+SSSE AA+ VVAADG+GNYKTV+EAV AAPSKNS
Sbjct: 241 SRRREDLENGIKWPEWMSIQDQMLLDSSSEEAADVVVAADGTGNYKTVSEAVKAAPSKNS 300

Query: 301 KRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFRSATLAAVGD 360
            RYIIKIKAG Y ENVDVPSSKRNIMFWGDGRSNT I ++RSHG+GWSTF SAT+ AVGD
Sbjct: 301 -RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITADRSHGSGWSTFNSATVVAVGD 360

Query: 361 GFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAG 420
           GFLARDITFQN AG+ANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFV C+VAG
Sbjct: 361 GFLARDITFQNTAGSANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVKCVVAG 420

Query: 421 TVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEP 480
           TVDFIFGNAAAV QNSD+TPRKPGP+Q NMVTAQSRTD+NQNTGIVIQKCRIKATSDLEP
Sbjct: 421 TVDFIFGNAAAVIQNSDLTPRKPGPNQNNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEP 480

Query: 481 VIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADIS 540
           VI+EFPSFLGRPWEEYARVVVMQT+I+NVIDKEGWSTWNG  K PY+AEYDNNGAGAD S
Sbjct: 481 VIKEFPSFLGRPWEEYARVVVMQTSITNVIDKEGWSTWNGDIKKPYFAEYDNNGAGADTS 540

Query: 541 GRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK 575
           GRV WSLVI+EA+AKTFTA PFI GA WL STGFPYQL LY 
Sbjct: 541 GRVSWSLVINEAEAKTFTAEPFIDGAGWLPSTGFPYQLGLYN 581

BLAST of CsGy7G013035 vs. NCBI nr
Match: XP_008458040.2 (PREDICTED: LOW QUALITY PROTEIN: pectinesterase-like [Cucumis melo])

HSP 1 Score: 947 bits (2449), Expect = 0.0
Identity = 488/583 (83.70%), Postives = 526/583 (90.22%), Query Frame = 0

Query: 1   MENNKLSLALLAILLLTNAIVVRANSNEN-------SVIETSCAITLYPQLCHSTISSIV 60
           MEN KLSLAL A LLLTNAI+VRANSNEN       S+IETSCAITLYPQLCHSTISSI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60

Query: 61  GTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIY 120
           GTSNLLSLKDI ++SLSVAMDAAK NN NIK LM S NNVSKRD+I LHDCV++TDRTIY
Sbjct: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKNLM-SFNNVSKRDRIPLHDCVKSTDRTIY 120

Query: 121 ELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENEH 180
           EL KAIE FREYPNK+SLT YADDLKTFLSSAITNQVTCLDG   DKTEK VLR+IEN H
Sbjct: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTH 180

Query: 181 IHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDE 240
           IH TKLCSNALALV+KLTT+IA+ DEK+LVVHDFPYKITSI SQ++D KIVLFSNQEE E
Sbjct: 181 IHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGE 240

Query: 241 NRRREELEDGIKWPKWMSIEDQKLLESSS-EAAAEAVVAADGSGNYKTVAEAVAAAPSKN 300
           N RRE+LE+GIKWP+WMSI+DQKLLESSS EAAA+ VVA DGSGNYKTV+EAV AAPSKN
Sbjct: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKN 300

Query: 301 SKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFRSATLAAVG 360
           SKRYIIKIKAG Y ENVDVPSSKRNIMFWGDGRSNT I + RSHG+GWSTF SAT+ AVG
Sbjct: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360

Query: 361 DGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVA 420
           DGFLARDI+F+N AG ANGQAVALRVGSDHSAFYRC ML YQDTLYVHSNRQFFVNC+VA
Sbjct: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420

Query: 421 GTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLE 480
           GTVDF+FGNAAAV QNSD+TPRKPGP+QRNMVTAQSRTD+NQNTGIVIQKCRIKATSDLE
Sbjct: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480

Query: 481 PVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADI 540
           PVI+EFPSFLGRPWEEYARVVVMQTTI+NVIDK+GWSTWNG  K PY+AEYDNNGAGA+ 
Sbjct: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540

Query: 541 SGRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK 575
           SGRVPWSLVI+EA AKTFTA  FIGGADWL STGFPYQL LY 
Sbjct: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 582

BLAST of CsGy7G013035 vs. NCBI nr
Match: TYK30782.1 (pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 909 bits (2348), Expect = 0.0
Identity = 473/583 (81.13%), Postives = 510/583 (87.48%), Query Frame = 0

Query: 1   MENNKLSLALLAILLLTNAIVVRANSNEN-------SVIETSCAITLYPQLCHSTISSIV 60
           MEN KLSLAL A LLLTNAI+VRANSNEN       S+IETSCAITLYPQLCHSTISSI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60

Query: 61  GTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIY 120
           GTSNLLSLKDI ++SLSVAMDAAK NN NIKKL +S NNVSKRD+I LHDCV++TDRTIY
Sbjct: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIY 120

Query: 121 ELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENEH 180
           EL KAIE FREYPNK+SLT YADDLKTFLSSAITNQVTCLDG   DKTEKRVLR+IEN H
Sbjct: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTH 180

Query: 181 IHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDE 240
           IH TKLCSNALALV+KLTT+IA+ DEK+L       K     S       VLFSNQEE E
Sbjct: 181 IHATKLCSNALALVKKLTTEIALIDEKNLK------KSRKARSH------VLFSNQEEGE 240

Query: 241 NRRREELEDGIKWPKWMSIEDQKLLESSS-EAAAEAVVAADGSGNYKTVAEAVAAAPSKN 300
           N RRE+LE+GIKWP+WMSI+DQKLLESSS EAA + VVA DGSGNYKTV+EAV AAPSKN
Sbjct: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKN 300

Query: 301 SKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFRSATLAAVG 360
           SKRYIIKIKAG Y ENVDVPSSKRNIMFWGDGRSNT I + RSHG+GWSTF SAT+ AVG
Sbjct: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360

Query: 361 DGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVA 420
           DGFLARDI+F+N AG ANGQAVALRVGSDHSAFYRC ML YQDTLYVHSNRQFFVNC+VA
Sbjct: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420

Query: 421 GTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLE 480
           GTVDF+FGNAAAV QNSD+TPRKPGP+QRNMVTAQSRTD+NQNTGIVIQKCRIKATSDLE
Sbjct: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480

Query: 481 PVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADI 540
           PVI+EFPSFLGRPWEEYARVVVMQTTI+NVIDK+GWSTWNG  K PY+AEYDNNGAGA+ 
Sbjct: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540

Query: 541 SGRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK 575
           SGRVPWSLVI+EA AKTFTA  FIGGADWL STGFPYQL LY 
Sbjct: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 571

BLAST of CsGy7G013035 vs. NCBI nr
Match: KAA0026177.1 (pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 897 bits (2318), Expect = 0.0
Identity = 466/583 (79.93%), Postives = 504/583 (86.45%), Query Frame = 0

Query: 1   MENNKLSLALLAILLLTNAIVVRANSNEN-------SVIETSCAITLYPQLCHSTISSIV 60
           MEN KLSLAL A LLLTNAI+VRANSNEN       S+IETSCAITLYPQLCHSTISSI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60

Query: 61  GTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIY 120
           GTSNLLSLKDI ++SLSVAMDAAK NN NIKKL +S NNVSKRD+I LHDCV++TDRTIY
Sbjct: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIY 120

Query: 121 ELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENEH 180
           EL KAIE FREYPNK+SLT YADDLKTFLSSAITNQVTCLDG   DKTEKRVLR+IEN H
Sbjct: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTH 180

Query: 181 IHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDE 240
           IH TKLCSNALALV+KLTT+IA+ DEK+L                   K     + EE E
Sbjct: 181 IHATKLCSNALALVKKLTTEIALIDEKNL------------------KKSRKARSHEEGE 240

Query: 241 NRRREELEDGIKWPKWMSIEDQKLLESSS-EAAAEAVVAADGSGNYKTVAEAVAAAPSKN 300
           N RRE+LE+GIKWP+WMSI+DQKLLESSS EAA + VVA DGSGNYKTV+EAV AAPSKN
Sbjct: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKN 300

Query: 301 SKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFRSATLAAVG 360
           SKRYIIKIKAG Y ENVDVPSSKRNIMFWGDGRSNT I + RSHG+GWSTF SAT+ AVG
Sbjct: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360

Query: 361 DGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVA 420
           DGFLARDI+F+N AG ANGQAVALRVGSDHSAFYRC ML YQDTLYVHSNRQFFVNC+VA
Sbjct: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420

Query: 421 GTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLE 480
           GTVDF+FGNAAAV QNSD+TPRKPGP+QRNMVTAQSRTD+NQNTGIVIQKCRIKATSDLE
Sbjct: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480

Query: 481 PVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADI 540
           PVI+EFPSFLGRPWEEYARVVVMQTTI+NVIDK+GWSTWNG  K PY+AEYDNNGAGA+ 
Sbjct: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540

Query: 541 SGRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK 575
           SGRVPWSLVI+EA AKTFTA  FIGGADWL STGFPYQL LY 
Sbjct: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 565

BLAST of CsGy7G013035 vs. ExPASy TrEMBL
Match: A0A0A0K9Y3 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343870 PE=3 SV=1)

HSP 1 Score: 1124 bits (2907), Expect = 0.0
Identity = 573/575 (99.65%), Postives = 574/575 (99.83%), Query Frame = 0

Query: 1   MENNKLSLALLAILLLTNAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSNLLS 60
           MENNKLSLALLAILLLTNAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSNLLS
Sbjct: 1   MENNKLSLALLAILLLTNAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSNLLS 60

Query: 61  LKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIE 120
           LKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIE
Sbjct: 61  LKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIE 120

Query: 121 VFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENEHIHVTKLC 180
           VFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIEN HIHVTKLC
Sbjct: 121 VFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAHIHVTKLC 180

Query: 181 SNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREEL 240
           SNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREEL
Sbjct: 181 SNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREEL 240

Query: 241 EDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIKI 300
           EDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIKI
Sbjct: 241 EDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIKI 300

Query: 301 KAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFRSATLAAVGDGFLARDI 360
           KAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTF+SATLAAVGDGFLARDI
Sbjct: 301 KAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAVGDGFLARDI 360

Query: 361 TFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIFG 420
           TFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIFG
Sbjct: 361 TFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIFG 420

Query: 421 NAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFPS 480
           NAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFPS
Sbjct: 421 NAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFPS 480

Query: 481 FLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADISGRVPWSL 540
           FLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADISGRVPWSL
Sbjct: 481 FLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADISGRVPWSL 540

Query: 541 VIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK 575
           VIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK
Sbjct: 541 VIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK 575

BLAST of CsGy7G013035 vs. ExPASy TrEMBL
Match: A0A0A0K6S6 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343850 PE=3 SV=1)

HSP 1 Score: 957 bits (2475), Expect = 0.0
Identity = 491/582 (84.36%), Postives = 529/582 (90.89%), Query Frame = 0

Query: 1   MENNKLSLALLAILLLTNAIVVRANSNEN-------SVIETSCAITLYPQLCHSTISSIV 60
           MEN KLSLAL A LLLTNAI+VRANSNEN       SVIETSCAITLYPQLCHSTISSI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMNNILSVIETSCAITLYPQLCHSTISSIL 60

Query: 61  GTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIY 120
           GTSNLLSLKDI E+SLSVAMDAAK NN+NIKKL +S N V KRD+I LHDCV+TTDRTIY
Sbjct: 61  GTSNLLSLKDIVELSLSVAMDAAKLNNENIKKLSMSGNIVGKRDRIALHDCVKTTDRTIY 120

Query: 121 ELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENEH 180
           EL KAI+ F EYPNK+SL  YADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIEN H
Sbjct: 121 ELDKAIQDFSEYPNKKSLASYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENTH 180

Query: 181 IHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDE 240
             VTKLCSNALALV+KLTTD+A+TDEKSLVVHDFPYKITSI SQ+DDPKIVLFSNQE+ E
Sbjct: 181 NQVTKLCSNALALVKKLTTDVALTDEKSLVVHDFPYKITSISSQIDDPKIVLFSNQEDGE 240

Query: 241 NRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNS 300
           +RRRE+LE+GIKWP+WMSI+DQ LL+SSSE AA+ VVAADG+GNYKTV+EAV AAPSKNS
Sbjct: 241 SRRREDLENGIKWPEWMSIQDQMLLDSSSEEAADVVVAADGTGNYKTVSEAVKAAPSKNS 300

Query: 301 KRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFRSATLAAVGD 360
            RYIIKIKAG Y ENVDVPSSKRNIMFWGDGRSNT I ++RSHG+GWSTF SAT+ AVGD
Sbjct: 301 -RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITADRSHGSGWSTFNSATVVAVGD 360

Query: 361 GFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAG 420
           GFLARDITFQN AG+ANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFV C+VAG
Sbjct: 361 GFLARDITFQNTAGSANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVKCVVAG 420

Query: 421 TVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEP 480
           TVDFIFGNAAAV QNSD+TPRKPGP+Q NMVTAQSRTD+NQNTGIVIQKCRIKATSDLEP
Sbjct: 421 TVDFIFGNAAAVIQNSDLTPRKPGPNQNNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEP 480

Query: 481 VIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADIS 540
           VI+EFPSFLGRPWEEYARVVVMQT+I+NVIDKEGWSTWNG  K PY+AEYDNNGAGAD S
Sbjct: 481 VIKEFPSFLGRPWEEYARVVVMQTSITNVIDKEGWSTWNGDIKKPYFAEYDNNGAGADTS 540

Query: 541 GRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK 575
           GRV WSLVI+EA+AKTFTA PFI GA WL STGFPYQL LY 
Sbjct: 541 GRVSWSLVINEAEAKTFTAEPFIDGAGWLPSTGFPYQLGLYN 581

BLAST of CsGy7G013035 vs. ExPASy TrEMBL
Match: A0A1S3C861 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497582 PE=3 SV=1)

HSP 1 Score: 947 bits (2449), Expect = 0.0
Identity = 488/583 (83.70%), Postives = 526/583 (90.22%), Query Frame = 0

Query: 1   MENNKLSLALLAILLLTNAIVVRANSNEN-------SVIETSCAITLYPQLCHSTISSIV 60
           MEN KLSLAL A LLLTNAI+VRANSNEN       S+IETSCAITLYPQLCHSTISSI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60

Query: 61  GTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIY 120
           GTSNLLSLKDI ++SLSVAMDAAK NN NIK LM S NNVSKRD+I LHDCV++TDRTIY
Sbjct: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKNLM-SFNNVSKRDRIPLHDCVKSTDRTIY 120

Query: 121 ELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENEH 180
           EL KAIE FREYPNK+SLT YADDLKTFLSSAITNQVTCLDG   DKTEK VLR+IEN H
Sbjct: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTH 180

Query: 181 IHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDE 240
           IH TKLCSNALALV+KLTT+IA+ DEK+LVVHDFPYKITSI SQ++D KIVLFSNQEE E
Sbjct: 181 IHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGE 240

Query: 241 NRRREELEDGIKWPKWMSIEDQKLLESSS-EAAAEAVVAADGSGNYKTVAEAVAAAPSKN 300
           N RRE+LE+GIKWP+WMSI+DQKLLESSS EAAA+ VVA DGSGNYKTV+EAV AAPSKN
Sbjct: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKN 300

Query: 301 SKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFRSATLAAVG 360
           SKRYIIKIKAG Y ENVDVPSSKRNIMFWGDGRSNT I + RSHG+GWSTF SAT+ AVG
Sbjct: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360

Query: 361 DGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVA 420
           DGFLARDI+F+N AG ANGQAVALRVGSDHSAFYRC ML YQDTLYVHSNRQFFVNC+VA
Sbjct: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420

Query: 421 GTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLE 480
           GTVDF+FGNAAAV QNSD+TPRKPGP+QRNMVTAQSRTD+NQNTGIVIQKCRIKATSDLE
Sbjct: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480

Query: 481 PVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADI 540
           PVI+EFPSFLGRPWEEYARVVVMQTTI+NVIDK+GWSTWNG  K PY+AEYDNNGAGA+ 
Sbjct: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540

Query: 541 SGRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK 575
           SGRVPWSLVI+EA AKTFTA  FIGGADWL STGFPYQL LY 
Sbjct: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 582

BLAST of CsGy7G013035 vs. ExPASy TrEMBL
Match: A0A5D3E4E8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold343G001330 PE=3 SV=1)

HSP 1 Score: 909 bits (2348), Expect = 0.0
Identity = 473/583 (81.13%), Postives = 510/583 (87.48%), Query Frame = 0

Query: 1   MENNKLSLALLAILLLTNAIVVRANSNEN-------SVIETSCAITLYPQLCHSTISSIV 60
           MEN KLSLAL A LLLTNAI+VRANSNEN       S+IETSCAITLYPQLCHSTISSI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60

Query: 61  GTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIY 120
           GTSNLLSLKDI ++SLSVAMDAAK NN NIKKL +S NNVSKRD+I LHDCV++TDRTIY
Sbjct: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIY 120

Query: 121 ELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENEH 180
           EL KAIE FREYPNK+SLT YADDLKTFLSSAITNQVTCLDG   DKTEKRVLR+IEN H
Sbjct: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTH 180

Query: 181 IHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDE 240
           IH TKLCSNALALV+KLTT+IA+ DEK+L       K     S       VLFSNQEE E
Sbjct: 181 IHATKLCSNALALVKKLTTEIALIDEKNLK------KSRKARSH------VLFSNQEEGE 240

Query: 241 NRRREELEDGIKWPKWMSIEDQKLLESSS-EAAAEAVVAADGSGNYKTVAEAVAAAPSKN 300
           N RRE+LE+GIKWP+WMSI+DQKLLESSS EAA + VVA DGSGNYKTV+EAV AAPSKN
Sbjct: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKN 300

Query: 301 SKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFRSATLAAVG 360
           SKRYIIKIKAG Y ENVDVPSSKRNIMFWGDGRSNT I + RSHG+GWSTF SAT+ AVG
Sbjct: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360

Query: 361 DGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVA 420
           DGFLARDI+F+N AG ANGQAVALRVGSDHSAFYRC ML YQDTLYVHSNRQFFVNC+VA
Sbjct: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420

Query: 421 GTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLE 480
           GTVDF+FGNAAAV QNSD+TPRKPGP+QRNMVTAQSRTD+NQNTGIVIQKCRIKATSDLE
Sbjct: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480

Query: 481 PVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADI 540
           PVI+EFPSFLGRPWEEYARVVVMQTTI+NVIDK+GWSTWNG  K PY+AEYDNNGAGA+ 
Sbjct: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540

Query: 541 SGRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK 575
           SGRVPWSLVI+EA AKTFTA  FIGGADWL STGFPYQL LY 
Sbjct: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 571

BLAST of CsGy7G013035 vs. ExPASy TrEMBL
Match: A0A5A7SNA2 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G001370 PE=3 SV=1)

HSP 1 Score: 897 bits (2318), Expect = 0.0
Identity = 466/583 (79.93%), Postives = 504/583 (86.45%), Query Frame = 0

Query: 1   MENNKLSLALLAILLLTNAIVVRANSNEN-------SVIETSCAITLYPQLCHSTISSIV 60
           MEN KLSLAL A LLLTNAI+VRANSNEN       S+IETSCAITLYPQLCHSTISSI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60

Query: 61  GTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIY 120
           GTSNLLSLKDI ++SLSVAMDAAK NN NIKKL +S NNVSKRD+I LHDCV++TDRTIY
Sbjct: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIY 120

Query: 121 ELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENEH 180
           EL KAIE FREYPNK+SLT YADDLKTFLSSAITNQVTCLDG   DKTEKRVLR+IEN H
Sbjct: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTH 180

Query: 181 IHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDE 240
           IH TKLCSNALALV+KLTT+IA+ DEK+L                   K     + EE E
Sbjct: 181 IHATKLCSNALALVKKLTTEIALIDEKNL------------------KKSRKARSHEEGE 240

Query: 241 NRRREELEDGIKWPKWMSIEDQKLLESSS-EAAAEAVVAADGSGNYKTVAEAVAAAPSKN 300
           N RRE+LE+GIKWP+WMSI+DQKLLESSS EAA + VVA DGSGNYKTV+EAV AAPSKN
Sbjct: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKN 300

Query: 301 SKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFRSATLAAVG 360
           SKRYIIKIKAG Y ENVDVPSSKRNIMFWGDGRSNT I + RSHG+GWSTF SAT+ AVG
Sbjct: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360

Query: 361 DGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVA 420
           DGFLARDI+F+N AG ANGQAVALRVGSDHSAFYRC ML YQDTLYVHSNRQFFVNC+VA
Sbjct: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420

Query: 421 GTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLE 480
           GTVDF+FGNAAAV QNSD+TPRKPGP+QRNMVTAQSRTD+NQNTGIVIQKCRIKATSDLE
Sbjct: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480

Query: 481 PVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADI 540
           PVI+EFPSFLGRPWEEYARVVVMQTTI+NVIDK+GWSTWNG  K PY+AEYDNNGAGA+ 
Sbjct: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540

Query: 541 SGRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK 575
           SGRVPWSLVI+EA AKTFTA  FIGGADWL STGFPYQL LY 
Sbjct: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 565

BLAST of CsGy7G013035 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 530.0 bits (1364), Expect = 2.3e-150
Identity = 304/594 (51.18%), Postives = 391/594 (65.82%), Query Frame = 0

Query: 2   ENNKLSLALLAILLLTNAIVV-------RANS------NENSVIETSCAITLYPQLCHST 61
           +N KL L   A+ LL  A V        +AN       + ++V+ +SC+ T YP+LC S 
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISA 75

Query: 62  ISSIVGTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETT 121
           + +  G   L S KD+ E S+++ + A +HN   +KKL+     ++ R+K  LHDC+ET 
Sbjct: 76  VVT-AGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETI 135

Query: 122 DRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRL 181
           D T+ EL + +E    YP K++L  +A DLKT +SSAITNQ TCLDG SHD  +K+V + 
Sbjct: 136 DETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKA 195

Query: 182 IENEHIHVTKLCSNALALVQKLT-TDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFS 241
           +    IHV  +CSNALA+++ +T TDIA  ++K+        KITS   ++ +       
Sbjct: 196 LLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKA--------KITSNNRKLKE------E 255

Query: 242 NQEED---ENRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEA 301
           NQE     +     EL D   WP W+S  D++LL+ S    A+A VAADGSG +KTVA A
Sbjct: 256 NQETTVAVDIAGAGEL-DSEGWPTWLSAGDRRLLQGSG-VKADATVAADGSGTFKTVAAA 315

Query: 302 VAAAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFR 361
           VAAAP  ++KRY+I IKAG Y ENV+V   K+NIMF GDGR+ T I  +R+   G +TF 
Sbjct: 316 VAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFH 375

Query: 362 SATLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQ 421
           SAT+AAVG+ FLARDITFQN AG +  QAVALRVGSD SAFY C MLAYQDTLYVHSNRQ
Sbjct: 376 SATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQ 435

Query: 422 FFVNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCR 481
           FFV C++AGTVDFIFGNAA V Q+ DI  R+P   Q+NMVTAQ RTD NQNTGIVIQKCR
Sbjct: 436 FFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCR 495

Query: 482 IKATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNG--QRKSPYYAE 541
           I ATSDL+ V   FP++LGRPW+EY++ V+MQ+ IS+VI  EGWS W G     +  Y E
Sbjct: 496 IGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYRE 555

Query: 542 YDNNGAGADISGRVPW---SLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 574
           Y N GAGA  + RV W    ++   A+A+ +TAG FIGG  WLSSTGFP+ L L
Sbjct: 556 YSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of CsGy7G013035 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 524.6 bits (1350), Expect = 9.8e-149
Identity = 294/598 (49.16%), Postives = 390/598 (65.22%), Query Frame = 0

Query: 2   ENNK---LSLALLAILLLTNAIVVRA---NSNEN-----------SVIETSCAITLYPQL 61
           +NNK   LS A +A+LLL + + + A   N N+N           +++++ C+ TLYP+L
Sbjct: 15  KNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPEL 74

Query: 62  CHSTISSIVGTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDC 121
           C S +++  G   L S K++ E SL++   A KHN   +KKL+     ++ R+   LHDC
Sbjct: 75  CFSAVAA-TGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDC 134

Query: 122 VETTDRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKR 181
           +ET D T+ EL  A+E   +YP ++SL  +ADDLKT +SSAITNQ TCLDG S+D  +++
Sbjct: 135 LETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRK 194

Query: 182 VLRLIENEHIHVTKLCSNALALVQKLT-TDIA---ITDEKSLVVHDFPYKITSIPSQMDD 241
           V + +    +HV  +CSNALA+++ +T TDIA   + D+ S   ++   K+  +   +D 
Sbjct: 195 VRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDLD- 254

Query: 242 PKIVLFSNQEEDENRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKT 301
                                DG  WPKW+S+ D++LL+ S+   A+A VA DGSG++ T
Sbjct: 255 --------------------SDG--WPKWLSVGDRRLLQGST-IKADATVADDGSGDFTT 314

Query: 302 VAEAVAAAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGW 361
           VA AVAAAP K++KR++I IKAG Y ENV+V   K NIMF GDGR  T I  +R+   G 
Sbjct: 315 VAAAVAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGS 374

Query: 362 STFRSATLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVH 421
           +TF SAT+AAVG+ FLARDITFQN AG +  QAVALRVGSD SAFY+C M AYQDTLYVH
Sbjct: 375 TTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVH 434

Query: 422 SNRQFFVNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVI 481
           SNRQFFV C + GTVDFIFGNAAAV Q+ DI  R+P   Q+NMVTAQ R+D NQNTGIVI
Sbjct: 435 SNRQFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVI 494

Query: 482 QKCRIKATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQ--RKSP 541
           Q CRI  TSDL  V   FP++LGRPW+EY+R V+MQ+ IS+VI  EGW  W+G     + 
Sbjct: 495 QNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTL 554

Query: 542 YYAEYDNNGAGADISGRVPW---SLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 574
            Y EY N G GA  + RV W    ++  + +A+ FTAG FIGG  WL+STGFP+ LSL
Sbjct: 555 TYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of CsGy7G013035 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 340.5 bits (872), Expect = 2.6e-93
Identity = 227/592 (38.34%), Postives = 320/592 (54.05%), Query Frame = 0

Query: 2   ENNKLSLALLAIL-----LLTNAIVVRANSNENSVIETSCAITLYPQLCH-----STISS 61
           +N  L L+ +AIL          I V  N+N++S++ TS       Q+CH      +  +
Sbjct: 18  KNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLLTTS-------QICHGAHDQDSCQA 77

Query: 62  IVGTSNLLSLK-----DIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVE 121
           ++     LSL      D+  V L  ++   +     + +  + +N V  RDK G  DC E
Sbjct: 78  LLSEFTTLSLSKLNRLDLLHVFLKNSVWRLESTMTMVSEARIRSNGV--RDKAGFADCEE 137

Query: 122 TTDRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVL 181
             D +   +  ++E  R      +L  Y+ ++ T+LSS +TN +TCL+ +S         
Sbjct: 138 MMDVSKDRMMSSMEELR--GGNYNLESYS-NVHTWLSSVLTNYMTCLESIS--------- 197

Query: 182 RLIENEHIHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLF 241
                       +  N+  +V+    D+      +L +        S+    DD K+++ 
Sbjct: 198 -----------DVSVNSKQIVKPQLEDLVSRARVALAI------FVSVLPARDDLKMII- 257

Query: 242 SNQEEDENRRREELEDGIKWPKWMSIEDQKLLESSS---EAAAEAVVAADGSGNYKTVAE 301
           SN                ++P W++  D+KLLESS    +  A  VVA DG+G +KTV E
Sbjct: 258 SN----------------RFPSWLTALDRKLLESSPKTLKVTANVVVAKDGTGKFKTVNE 317

Query: 302 AVAAAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTF 361
           AVAAAP  ++ RY+I +K G Y E +D+   K+N+M  GDG+  T I  + +   G +TF
Sbjct: 318 AVAAAPENSNTRYVIYVKKGVYKETIDIGKKKKNLMLVGDGKDATIITGSLNVIDGSTTF 377

Query: 362 RSATLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNR 421
           RSAT+AA GDGF+A+DI FQN AG A  QAVALRV +D +   RC + AYQDTLY H+ R
Sbjct: 378 RSATVAANGDGFMAQDIWFQNTAGPAKHQAVALRVSADQTVINRCRIDAYQDTLYTHTLR 437

Query: 422 QFFVNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKC 481
           QF+ +  + GTVDFIFGN+A VFQN DI  R PG  Q+NM+TAQ R D NQNT I IQKC
Sbjct: 438 QFYRDSYITGTVDFIFGNSAVVFQNCDIVARNPGAGQKNMLTAQGREDQNQNTAISIQKC 497

Query: 482 RIKATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQ--RKSPYYA 541
           +I A+SDL PV     +FLGRPW+ Y+R V+MQ+ I N ID  GW  W+G+    + YY 
Sbjct: 498 KITASSDLAPVKGSVKTFLGRPWKLYSRTVIMQSFIDNHIDPAGWFPWDGEFALSTLYYG 554

Query: 542 EYDNNGAGADISGRVPW---SLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQ 571
           EY N G GAD S RV W    ++ D  +A+ FT    I G  WL  TG  +Q
Sbjct: 558 EYANTGPGADTSKRVNWKGFKVIKDSKEAEQFTVAKLIQGGLWLKPTGVTFQ 554

BLAST of CsGy7G013035 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 335.1 bits (858), Expect = 1.1e-91
Identity = 199/488 (40.78%), Postives = 278/488 (56.97%), Query Frame = 0

Query: 92  NVSKRDKIGLHDCVETTDRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVT 151
           N++  ++    DC+   D TI +L  A+       + RS +L  +D+   L++ +T Q T
Sbjct: 85  NLTHYERCAFEDCLGLLDDTISDLETAVS------DLRSSSLEFNDISMLLTNVMTYQDT 144

Query: 152 CLDGLSHDKTEKRVLRLIENEHIHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKI 211
           CLDG S            EN +    +L  N    ++++  DI+     SL      + +
Sbjct: 145 CLDGFSTSDN--------ENNNDMTYELPEN----LKEIILDISNNLSNSL------HML 204

Query: 212 TSIPSQMDDPKIVLFSNQEEDENRRREELEDGIKWPKWMSIEDQKLLESS-SEAAAEAVV 271
             I  +   PK                  E  +++P W+S  DQ+LLE+   E      V
Sbjct: 205 QVISRKKPSPK----------------SSEVDVEYPSWLSENDQRLLEAPVQETNYNLSV 264

Query: 272 AADGSGNYKTVAEAVAAAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKI 331
           A DG+GN+ T+ +AV AAP+ +  R+II IK GEY+ENV++P  K  IMF GDG   T I
Sbjct: 265 AIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVI 324

Query: 332 ISNRSHGTGWSTFRSATLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSM 391
            +NRS   GWSTF++ T+   G G++A+DI+F N AG A  QAVA R GSDHSAFYRC  
Sbjct: 325 KANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEF 384

Query: 392 LAYQDTLYVHSNRQFFVNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRT 451
             YQDTLYVHS +QF+  C + GT+DFIFGNAA VFQNS +  RKP P  +   TAQSR 
Sbjct: 385 DGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRN 444

Query: 452 DINQNTGIVIQKCRIKATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWST 511
             +Q TGI I  CRI A  DL PV E F ++LGRPW +Y+R V++++ I ++I   GW  
Sbjct: 445 QSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGW-- 504

Query: 512 WNGQR----KSPYYAEYDNNGAGADISGRVPW---SLVIDEAQAKTFTAGPFIGGADWLS 571
             G++    ++ YY EY N G GA+++ RV W     + ++ +A  FT GPFI G+ WL+
Sbjct: 505 LEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLN 530

BLAST of CsGy7G013035 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 330.5 bits (846), Expect = 2.7e-90
Identity = 212/539 (39.33%), Postives = 301/539 (55.84%), Query Frame = 0

Query: 32  IETSCAITLYPQLCHSTISSIVGTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTN 91
           ++  C+ T  P+ C   ++       + S  +  ++S+ + +D A     +   L     
Sbjct: 30  VKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHAFTLGPKCR 89

Query: 92  NVSKRDKIGLHDCVETTDRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVT 151
           +   R+K    DC++  D T+ ++ + ++     PN +   L   D +T+LS+A+TN  T
Sbjct: 90  DT--REKAAWEDCIKLYDLTVSKINETMD-----PNVKCSKL---DAQTWLSTALTNLDT 149

Query: 152 CLDGLSHDKTEKRVLRLIENEHIHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKI 211
           C  G         VL L+ N   +V+ L  N LA                         I
Sbjct: 150 CRAGFLELGVTDIVLPLMSN---NVSNLLCNTLA-------------------------I 209

Query: 212 TSIPSQMDDPKIVLFSNQEEDENRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVA 271
             +P     P+                  +DG  +P W+   D+KLL+SS+     AVVA
Sbjct: 210 NKVPFNYTPPE------------------KDG--FPSWVKPGDRKLLQSST-PKDNAVVA 269

Query: 272 ADGSGNYKTVAEAVAAAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKII 331
            DGSGN+KT+ EA+ AA    S R++I +K G Y EN+++   K+N+M  GDG   T I 
Sbjct: 270 KDGSGNFKTIKEAIDAA--SGSGRFVIYVKQGVYSENLEI--RKKNVMLRGDGIGKTIIT 329

Query: 332 SNRSHGTGWSTFRSATLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSML 391
            ++S G G +TF SAT+AAVGDGF+AR ITF+N AGA+N QAVALR GSD S FY+CS  
Sbjct: 330 GSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFE 389

Query: 392 AYQDTLYVHSNRQFFVNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTD 451
           AYQDTLYVHSNRQF+ +C V GTVDFIFGNAAAV QN +I  R+P  S+ N +TAQ R+D
Sbjct: 390 AYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP-RSKTNTITAQGRSD 449

Query: 452 INQNTGIVIQKCRIKATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTW 511
            NQNTGI+I   R+ A SDL PV+    ++LGRPW +Y+R V M+T++ ++ID  GW  W
Sbjct: 450 PNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEW 504

Query: 512 NGQ--RKSPYYAEYDNNGAGADISGRVPW---SLVIDEAQAKTFTAGPFIGGADWLSST 566
           +G    K+ +YAE+ N G GA  SGRV W    ++   ++A  FT G F+ G  W+ S+
Sbjct: 510 DGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS 504

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O048862.6e-15451.35Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1[more]
Q431437.6e-15451.78Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PM... [more]
P839483.8e-15351.01Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1[more]
O490063.3e-14951.18Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... [more]
Q425341.4e-14749.16Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_004139620.20.099.65pectinesterase [Cucumis sativus] >KGN44621.1 hypothetical protein Csa_015743 [Cu... [more]
XP_011659194.10.084.36pectinesterase [Cucumis sativus] >KGN44619.1 hypothetical protein Csa_015815 [Cu... [more]
XP_008458040.20.083.70PREDICTED: LOW QUALITY PROTEIN: pectinesterase-like [Cucumis melo][more]
TYK30782.10.081.13pectinesterase-like [Cucumis melo var. makuwa][more]
KAA0026177.10.079.93pectinesterase-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A0A0K9Y30.099.65Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343870 PE=3 SV=1[more]
A0A0A0K6S60.084.36Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343850 PE=3 SV=1[more]
A0A1S3C8610.083.70Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497582 PE=3 SV=1[more]
A0A5D3E4E80.081.13Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold343G00133... [more]
A0A5A7SNA20.079.93Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G001370... [more]
Match NameE-valueIdentityDescription
AT3G14310.12.3e-15051.18pectin methylesterase 3 [more]
AT1G53830.19.8e-14949.16pectin methylesterase 2 [more]
AT1G11580.12.6e-9338.34methylesterase PCR A [more]
AT4G02300.11.1e-9140.78Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G45220.12.7e-9039.33Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 27..186
e-value: 3.9E-29
score: 112.8
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 6..191
e-value: 2.8E-24
score: 83.4
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 31..186
e-value: 2.9E-25
score: 89.4
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 268..558
e-value: 3.3E-124
score: 413.4
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 261..573
e-value: 2.7E-115
score: 387.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 26..192
e-value: 9.1E-30
score: 105.6
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 30..190
NoneNo IPR availablePANTHERPTHR31707:SF3PECTINESTERASE/PECTINESTERASE INHIBITOR 3coord: 26..571
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 26..571
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 34..192
e-value: 5.40631E-34
score: 124.473
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 411..420
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 263..570

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy7G013035.1CsGy7G013035.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0110165 cellular anatomical entity
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity