CsGy7G004550 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy7G004550
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	Diacylglycerol O-acyltransferase
Location	Gy14Chr7: 3412603 .. 3413760 (+)
RNA-Seq Expression	CsGy7G004550
Synteny	CsGy7G004550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAAAGGAGAGAAGAAGTGGAAAAGGTAAACATAGAAGTGGAAGAACATGTGAAAGATCCAATCTATCCACCTGGTTTAACTATATAGAATCATACAAAGCTTATTGTGATGAGTACTTAACGAAGATATCCATGGAAGAATTTCGACCCGACAAACCGCTATGGGAAGCTCATTTGTTCAAGTATCCAACATCCCCGACCGTCGCTGGAACCATGGTTCTAAAGGTCCACCACTCCCTCGGCGATGGCTATTCTCTCATGGGTGTCGTTCTTTCTTGTCTTCACACAGCTTCAGACCTAACGTTCCTCTCACTTTTCCTTCCCGACGTCAACGACGATTCAAGGACGGTGTTGTGAAGTCTTTGACTGGAATTGTTTCCGCTGTTTGTAGAACGGCGTCGAATTTGAGGTAGAGTGTTTTGAAAGGAATAGATGAGAAAACTTCTATAAGGTCAGGACGTGAAGGGCTACAATTCATGCCGATGAATACTTTTACGGTGTCCTTTTCGCTCAATTACATAAAGAAAATCAAGACGAAGCTCGACGTGGTGAGTTTTGTAATGAAAAACGCATAACTCTATATTGAAGCTGCGAAAAAAAATATTCATATCTGTGTGTCCATGCCTGCATACACCTACCTCAACTGAATGTCAAGAAAATTTATAAAACGATATCATCATGAATTAATTGAATTCATCCTTCTCAATTATGTATTAAACTCGCATAACCCTTTTAACCACTAGAGTTATAACAATTTATTTTAGTCTTTCAGTTTTCGAAATGCCCAATTTAGCCATTAGTTCAGCTTTTTAAGAAAAAAAAAAGTACCATTGTTTGGTCGTTACCATATGATCTTGTCACGTCAAATTTGCCTCAATGGCTTGAATACATATTCATATTAGGTACATGACTATGCATATATGTTATTTATTTTATACTAACGAGTGTTATTTTTTTTTTCACTGAAAAATGCAGACAGTAAATGATGTGATAACAGGGATAACCTTCATGGGACTAAGGTTGTACATGGAAGAAATGGAGAAAAATTCCGGTGAAGTCGCAGCGACTGCATTGACAATAGTGAACACGAGAGTGATTGGAACCTACCGGCCGGCGACAGAGATGGCAAAACCAGAGAGCAAAGGACTATGGTGA

mRNA sequence

ATGAGAAAGGAGAGAAGAAGTGGAAAAGGTAAACATAGAAGTGGAAGAACATGTGAAAGATCCAATCTATCCACCTGGTTTAACTATATAGAATCATACAAAGCTTATTGTGATGAGTACTTAACGAAGATATCCATGGAAGAATTTCGACCCGACAAACCGCTATGGGAAGCTCATTTGTTCAAGTATCCAACATCCCCGACCGTCGCTGGAACCATGGTTCTAAAGGTCCACCACTCCCTCGGCGATGGCTATTCTCTCATGGGTGTCGTTCTTTCTTGTCTTCACACAGCTTCAGACCTAACGTTCCTCTCACTTTTCCTTCCCGACGTCAACGACGATTCAAGGACGAGTGTTTTGAAAGGAATAGATGAGAAAACTTCTATAAGGTCAGGACGTGAAGGGCTACAATTCATGCCGATGAATACTTTTACGGTGTCCTTTTCGCTCAATTACATAAAGAAAATCAAGACGAAGCTCGACGTGACAGTAAATGATGTGATAACAGGGATAACCTTCATGGGACTAAGGTTGTACATGGAAGAAATGGAGAAAAATTCCGGTGAAGTCGCAGCGACTGCATTGACAATAGTGAACACGAGAGTGATTGGAACCTACCGGCCGGCGACAGAGATGGCAAAACCAGAGAGCAAAGGACTATGGTGA

Coding sequence (CDS)

ATGAGAAAGGAGAGAAGAAGTGGAAAAGGTAAACATAGAAGTGGAAGAACATGTGAAAGATCCAATCTATCCACCTGGTTTAACTATATAGAATCATACAAAGCTTATTGTGATGAGTACTTAACGAAGATATCCATGGAAGAATTTCGACCCGACAAACCGCTATGGGAAGCTCATTTGTTCAAGTATCCAACATCCCCGACCGTCGCTGGAACCATGGTTCTAAAGGTCCACCACTCCCTCGGCGATGGCTATTCTCTCATGGGTGTCGTTCTTTCTTGTCTTCACACAGCTTCAGACCTAACGTTCCTCTCACTTTTCCTTCCCGACGTCAACGACGATTCAAGGACGAGTGTTTTGAAAGGAATAGATGAGAAAACTTCTATAAGGTCAGGACGTGAAGGGCTACAATTCATGCCGATGAATACTTTTACGGTGTCCTTTTCGCTCAATTACATAAAGAAAATCAAGACGAAGCTCGACGTGACAGTAAATGATGTGATAACAGGGATAACCTTCATGGGACTAAGGTTGTACATGGAAGAAATGGAGAAAAATTCCGGTGAAGTCGCAGCGACTGCATTGACAATAGTGAACACGAGAGTGATTGGAACCTACCGGCCGGCGACAGAGATGGCAAAACCAGAGAGCAAAGGACTATGGTGA

Protein sequence

MRKERRSGKGKHRSGRTCERSNLSTWFNYIESYKAYCDEYLTKISMEEFRPDKPLWEAHLFKYPTSPTVAGTMVLKVHHSLGDGYSLMGVVLSCLHTASDLTFLSLFLPDVNDDSRTSVLKGIDEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYMEEMEKNSGEVAATALTIVNTRVIGTYRPATEMAKPESKGLW*

Homology

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BLAST of CsGy7G004550 vs. ExPASy Swiss-Prot
Match: Q9M3B3 (Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=WSD4 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 65/242 (26.86%), Postives = 102/242 (42.15%), Query Frame = 0


Query: 30  IESYKAYCDEYLTKISMEEFRPDKPLWEAHLFKYPTSPTVAGTMVLKVHHSLGDGYSLMG 89
           IE+  A+ ++Y++ +        +PLWE HL    TS       VLK+HHS+GDG S+M 
Sbjct: 101 IENSNAFLEDYVSDLMKIPLDTSRPLWELHLLDLKTS-DAENVAVLKIHHSVGDGMSIMS 160

Query: 90  VVLSCLHTASDLTFL-------------SLFLPDVNDDSR---------TSVLKGI---- 149
           +VL+C+   S+   L             SL       DSR         T+V+ G+    
Sbjct: 161 LVLACMRKTSNPDELPSLPYQYRSSSRSSLLTTGSRSDSRLLWLVKVIWTAVILGLNTVC 220

Query: 150 -------------DEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITG 209
                        D +T I+      +   +     + SL+ IK  K  +++T+NDV+ G
Sbjct: 221 DALEFIVTTLFVKDTETPIKGDFLSTKSKQLRLVHRTVSLDDIKLTKNAMNMTINDVVLG 280

Query: 210 ITFMGL-----RLYMEEMEKNSGE------VAATALTIVNTRVIGTYRPATEMAKPESKG 222
           +T  GL     R Y EE  KN  +      +   +  +VN R     +   +M +  SK 
Sbjct: 281 VTQAGLSRYLARRYGEEETKNRKQKKLPKRIRLRSALLVNLRPTTGIQDLADMMEKGSKC 340

[Close]

BLAST of CsGy7G004550 vs. ExPASy Swiss-Prot
Match: Q94CK0 (Wax ester synthase/diacylglycerol acyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=WSD7 PE=1 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 5.5e-12
Identity = 61/242 (25.21%), Postives = 102/242 (42.15%), Query Frame = 0


Query: 31  ESYKAYCDEYLTKISMEEFRPDKPLWEAHLFKYPTSPTVAGTMVLKVHHSLGDGYSLMGV 90
           E  +++ D+Y+++++M      +PLW+ H+    TS   A    ++ HHSLGDG SL+ +
Sbjct: 88  EGGQSFIDDYMSRLTMIPLDRSRPLWDIHILNVKTSEAEA-VGFIRSHHSLGDGMSLISL 147

Query: 91  VLSCLHTASDLTFLSLFLPDVNDDSRTS-------------------------------- 150
           +L+C H  SD    S  +P +   +  S                                
Sbjct: 148 MLACTHKTSDPDMFSNAIPSMKRRATMSHSLKTKGWFLRSIFTIGSTMRLLWNTTIDMLL 207

Query: 151 ----VLKGIDEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFM 210
               VL   D KT +++G + ++  P   +    SL+ IK IK  +++T+NDV+ GIT  
Sbjct: 208 LLATVLFLKDTKTPLKAGAD-VRSNPKRFYHRIISLDDIKLIKNAMNMTINDVLFGITQA 267

Query: 211 GLRLYMEEM--EKNSGEVAATA-------------LTIVNTRVIGTYRPATEMAKPESKG 222
            L  Y+      K   + A T+                VN R    ++P  +M   +SK 
Sbjct: 268 SLSHYLNRQYGTKKEEDGALTSYRNNLPDGIRFRVACTVNLRSDIGFKPLADMMVKDSKC 327

[Close]

BLAST of CsGy7G004550 vs. ExPASy Swiss-Prot
Match: Q9FFE8 (Wax ester synthase/diacylglycerol acyltransferase 8 OS=Arabidopsis thaliana OX=3702 GN=WSD8 PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 5.5e-12
Identity = 62/244 (25.41%), Postives = 103/244 (42.21%), Query Frame = 0


Query: 31  ESYKAYCDEYLTKISMEEFRPDKPLWEAHLFKYPTSPTVAGTMVLKVHHSLGDGYSLMGV 90
           E  +++ D+Y++++++      +PLW+ H+    TS   A   V++ +H+L DG S + +
Sbjct: 88  EGGQSFVDDYISRLTLIPLDRSRPLWDIHILNVKTSYAEA-VGVIRFNHALADGMSFISL 147

Query: 91  VLSCLHTASDLTFLSLFLPDVNDDSRTS-------------------------------- 150
           VL+C H  S+   LS  +P V   S  S                                
Sbjct: 148 VLACTHKTSNPDMLSTAIPSVKRRSTVSHSLKKTGWFLTAIFTIGSTMRLIWNTLVDMFL 207

Query: 151 ----VLKGIDEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFM 210
               +L   D KT ++ G   ++  P   +  + SL+ IK IK  +++T+NDV+ GIT  
Sbjct: 208 LFATMLFLKDTKTPLKGG-ANVRSNPKTFYHRNISLDDIKLIKNAMNMTINDVLLGITQA 267

Query: 211 GLRLYME---EMEKNSGE--------------VAATALTIVNTRVIGTYRPATEMAKPES 222
            L  Y+    E E N+ E              +   A   VN R    ++P  EM   +S
Sbjct: 268 ALSSYLNRRYEHENNNEEDGVLTSYTNNLPDRIRFRAGCTVNLRSDIGFKPLAEMMVKDS 327

[Close]

BLAST of CsGy7G004550 vs. ExPASy Swiss-Prot
Match: Q5KS41 (Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=3702 GN=WSD11 PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 4.7e-11
Identity = 62/236 (26.27%), Postives = 96/236 (40.68%), Query Frame = 0


Query: 30  IESYKAYCDEYLTKISMEEFRPDKPLWEAHLFKYPTSPTVAGTMVLKVHHSLGDGYSLMG 89
           IE+   Y ++Y++K++       KPLWE HL    TS       +LK+HHSLGDG SLM 
Sbjct: 96  IENPDQYLEDYISKLTTIPMDLSKPLWEMHLLGVKTS-NAESYAILKIHHSLGDGMSLMS 155

Query: 90  VVLSCLHTASDLTFL-------SLFLPDVNDDSRTSV---LKGI---------------- 149
           ++L+C    SD   L         F P  N      +     G+                
Sbjct: 156 LLLACTRKTSDPEALPTVAVHKKRFGPSCNSGFFNKIWWLFVGLWFILRLLFNTFVDILM 215

Query: 150 ---------DEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFM 209
                    D +T + + + G + +P        S + +K +K  + +TVNDV+ G+T  
Sbjct: 216 FALTIFVLRDTETPLLA-KPGSELIPKRFVHRIISFDDVKLVKNAMKMTVNDVLLGVTQA 275

Query: 210 GL-----RLYMEEMEKNSGE----VAATALTIVNTRVIGTYRPATEMAKPESKGLW 222
           GL     R Y +E    S E    +   +  ++N R         +M   +SK  W
Sbjct: 276 GLSRYLSRKYDQEATPKSKESMRRIRLRSAIMINLRPNAGIEALADMMAKKSKCRW 329

[Close]

BLAST of CsGy7G004550 vs. ExPASy Swiss-Prot
Match: Q9M3B2 (Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=WSD5 PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 5.2e-10
Identity = 64/245 (26.12%), Postives = 98/245 (40.00%), Query Frame = 0


Query: 29  YIESYKA--YCDEYLTKISMEEFRPDKPLWEAHLFKYPTSPTVAGTMVLKVHHSLGDGYS 88
           +IE+  A  + + Y++ ++       KPLWE HL    TS       VL++HHSLGDG S
Sbjct: 110 HIENANADVFLESYVSDLTTIPLDTSKPLWEVHLLDLKTS-DAENVAVLRIHHSLGDGMS 169

Query: 89  LMGVVLSCLHTASDLTFLSLFLPDVNDDSRTSVLKGIDEKTS----------------IR 148
           +M +VL+C    S+   L          S +S LK      S                + 
Sbjct: 170 MMSLVLACTRKTSNPNELPSLPYQNRPSSGSSSLKTSSRCYSRFFWLVMVLWSAALLVLN 229

Query: 149 SGREGLQFMPMNTFTV-----------------------SFSLNYIKKIKTKLDVTVNDV 208
           +  + L+F+    F                         + SL+ IK IK  + +TVNDV
Sbjct: 230 TVCDALEFIATALFLKDTETPIKGDFKLSKGKRMCMVHRTVSLDDIKLIKNAMKMTVNDV 289

Query: 209 ITGITFMGLRLYM-------EEMEKNSGE----VAATALTIVNTRVIGTYRPATEMAKPE 222
           + G++  GL  Y+       EE ++NS      +   A  +VN R     +   +M    
Sbjct: 290 VLGVSQAGLSQYLKRRYGEQEESKRNSSNIPKGIRLRAALLVNLRPTTGIQDLADMMTKG 349

[Close]

BLAST of CsGy7G004550 vs. NCBI nr
Match: XP_008455536.1 (PREDICTED: O-acyltransferase WSD1-like [Cucumis melo] >KAA0031532.1 O-acyltransferase WSD1-like [Cucumis melo var. makuwa] >TYK06985.1 O-acyltransferase WSD1-like [Cucumis melo var. makuwa])

HSP 1 Score: 313 bits (801), Expect = 5.35e-102
Identity = 168/214 (78.50%), Postives = 173/214 (80.84%), Query Frame = 0


Query: 30  IESYKAYCDEYLTKISMEEFRPDKPLWEAHLFKYPTSPTVAGTMVLKVHHSLGDGYSLMG 89
           IESYKA+ DEYLTKISMEEFRPDKPLWE HLFKYPTSPTVAGTM+LK+HHSLGDGYSLMG
Sbjct: 92  IESYKAHFDEYLTKISMEEFRPDKPLWETHLFKYPTSPTVAGTMILKLHHSLGDGYSLMG 151

Query: 90  VVLSCLHTASD----LTFLSLFLPDVND------------------DSRTSVLKGIDEKT 149
           VVLSCLHTASD    LTF S      N+                  D   SV KGIDEKT
Sbjct: 152 VVLSCLHTASDPNVPLTFPSRRQRRFNNGVVKSLTGIVSAVCRTASDLGWSVFKGIDEKT 211

Query: 150 SIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYMEEMEKNS 209
            IRSGREGLQFMPMNTFTVSFSLN IKKIKTKL VTVNDVITGI FMGLRLYMEE+EKNS
Sbjct: 212 PIRSGREGLQFMPMNTFTVSFSLNDIKKIKTKLQVTVNDVITGIIFMGLRLYMEEIEKNS 271

Query: 210 GEVAATALTIVNTRVIGTYRPATEMAKPESKGLW 221
           GEVAATALTIVNTRVIG YR ATEMAKPESKGLW
Sbjct: 272 GEVAATALTIVNTRVIGNYRSATEMAKPESKGLW 305

[Close]

BLAST of CsGy7G004550 vs. NCBI nr
Match: KAE8645787.1 (hypothetical protein Csa_017378, partial [Cucumis sativus])

HSP 1 Score: 244 bits (623), Expect = 9.06e-80
Identity = 117/117 (100.00%), Postives = 117/117 (100.00%), Query Frame = 0


Query: 1   MRKERRSGKGKHRSGRTCERSNLSTWFNYIESYKAYCDEYLTKISMEEFRPDKPLWEAHL 60
           MRKERRSGKGKHRSGRTCERSNLSTWFNYIESYKAYCDEYLTKISMEEFRPDKPLWEAHL
Sbjct: 1   MRKERRSGKGKHRSGRTCERSNLSTWFNYIESYKAYCDEYLTKISMEEFRPDKPLWEAHL 60

Query: 61  FKYPTSPTVAGTMVLKVHHSLGDGYSLMGVVLSCLHTASDLTFLSLFLPDVNDDSRT 117
           FKYPTSPTVAGTMVLKVHHSLGDGYSLMGVVLSCLHTASDLTFLSLFLPDVNDDSRT
Sbjct: 61  FKYPTSPTVAGTMVLKVHHSLGDGYSLMGVVLSCLHTASDLTFLSLFLPDVNDDSRT 117

[Close]

BLAST of CsGy7G004550 vs. NCBI nr
Match: XP_031260551.1 (LOW QUALITY PROTEIN: O-acyltransferase WSD1-like [Pistacia vera])

HSP 1 Score: 196 bits (498), Expect = 2.10e-56
Identity = 104/222 (46.85%), Postives = 142/222 (63.96%), Query Frame = 0


Query: 31  ESYKAYCDEYLTKISMEEFRPDKPLWEAHLFKYPTSPTVAGTMVLKVHHSLGDGYSLMGV 90
           ESY  Y DEY++KI MEEF   +PLWE H+FKYPT+ T  G ++ K+HHSLGDG+SLMG 
Sbjct: 91  ESYDNYLDEYISKIGMEEFAKSRPLWEIHIFKYPTT-TAPGVLIFKLHHSLGDGFSLMGA 150

Query: 91  VLSCLHTASD----LTFLSLFLP--DVNDDSRTSVLKGI--------------------- 150
           +LS L  A D    LTF S+ LP  + N D+  S+LK +                     
Sbjct: 151 LLSYLQRADDPSLPLTFPSVQLPSHNRNGDNNNSILKILPKIFSVVSKTVSDFCWSVTKS 210

Query: 151 ----DEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLY 210
               D+KT+IRSG +G++F P+N  T+SFSL++IKKIK  +  T+NDVITG  F+G RLY
Sbjct: 211 TVVEDDKTTIRSGDDGVEFRPINMATMSFSLDHIKKIKANVGATLNDVITGTIFLGTRLY 270

Query: 211 MEEMEKNSGEVAATALTIVNTRVIGTYRPATEMAKPESKGLW 221
           M+EM K S +  +TAL ++NTRV+ +Y+P  +M +P +K  W
Sbjct: 271 MQEMSKESSKAQSTALVLLNTRVLRSYKPVKDMVEPNAKTPW 311

[Close]

BLAST of CsGy7G004550 vs. NCBI nr
Match: XP_031280043.1 (O-acyltransferase WSD1-like [Pistacia vera])

HSP 1 Score: 191 bits (486), Expect = 1.66e-54
Identity = 102/222 (45.95%), Postives = 139/222 (62.61%), Query Frame = 0


Query: 31  ESYKAYCDEYLTKISMEEFRPDKPLWEAHLFKYPTSPTVAGTMVLKVHHSLGDGYSLMGV 90
           ESY  Y DEY++KI MEEF   +PLWE H+FKYPT+ +  G ++ K+HHSLGDG SL G 
Sbjct: 104 ESYDNYLDEYISKIGMEEFAKSRPLWEIHIFKYPTT-SAPGVLIFKLHHSLGDGLSLRGA 163

Query: 91  VLSCLHTASD----LTFLSLFLPDVNDD--SRTSVLKGI--------------------- 150
           +LSCL  A D    LTF S+ LP  N +  +  S+LK +                     
Sbjct: 164 LLSCLQRADDPSLPLTFPSVQLPSHNRNGGNNNSILKILPKIFSVVSKTVSDFSWSVTKS 223

Query: 151 ----DEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLY 210
               D+KT IRSG +G++F P+N  T+SFSL++IKKIK  +  T+NDVITG  F+G RLY
Sbjct: 224 TVVEDDKTPIRSGDDGVEFRPINMATMSFSLDHIKKIKANVGATLNDVITGTIFLGTRLY 283

Query: 211 MEEMEKNSGEVAATALTIVNTRVIGTYRPATEMAKPESKGLW 221
           M+EM K S +  +TAL ++NTRV+ +Y+P  +M +P +K  W
Sbjct: 284 MQEMSKESSKAQSTALVLLNTRVLRSYKPVKDMVEPNAKTPW 324

[Close]

BLAST of CsGy7G004550 vs. NCBI nr
Match: XP_031280304.1 (O-acyltransferase WSD1-like [Pistacia vera])

HSP 1 Score: 183 bits (464), Expect = 2.37e-51
Identity = 98/221 (44.34%), Postives = 136/221 (61.54%), Query Frame = 0


Query: 31  ESYKAYCDEYLTKISMEEFRPDKPLWEAHLFKYPTSPTVAGTMVLKVHHSLGDGYSLMGV 90
           E Y  Y D+Y++KI ME+FR  +PLWE H+ KYPT+ T A  M+ K+HHSLGDG+SLMG 
Sbjct: 92  EFYDKYFDDYISKIGMEQFRRTRPLWEIHVIKYPTT-TAAAVMIFKLHHSLGDGFSLMGA 151

Query: 91  VLSCLHTASD----LTFLSLFL-PDVNDDSRTSVLKGI---------------------- 150
           +LSCL  A +    LTF S+ L  + N  +  ++LK +                      
Sbjct: 152 LLSCLQRADNPSLPLTFPSVHLHSNRNGSNNNNILKSVSKVLSVISMTVSDFSWSIIKST 211

Query: 151 ---DEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYM 210
              D+KT IRSG  G++F P  T T+ FSL+ I++IKTKL  T+NDVITGI F+G RLYM
Sbjct: 212 LVEDDKTPIRSGDGGIEFRPTTTATMIFSLDKIRQIKTKLRATINDVITGIIFLGTRLYM 271

Query: 211 EEMEKNSGEVAATALTIVNTRVIGTYRPATEMAKPESKGLW 221
           +EM ++S +  +TA+ ++NTRV  +Y    +M KP +K  W
Sbjct: 272 QEMSQDSSKAQSTAVILLNTRVFRSYESVKDMVKPNAKAPW 311

[Close]

BLAST of CsGy7G004550 vs. ExPASy TrEMBL
Match: A0A0A0K3V2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G051340 PE=4 SV=1)

HSP 1 Score: 445 bits (1145), Expect = 4.44e-157
Identity = 221/221 (100.00%), Postives = 221/221 (100.00%), Query Frame = 0


Query: 1   MRKERRSGKGKHRSGRTCERSNLSTWFNYIESYKAYCDEYLTKISMEEFRPDKPLWEAHL 60
           MRKERRSGKGKHRSGRTCERSNLSTWFNYIESYKAYCDEYLTKISMEEFRPDKPLWEAHL
Sbjct: 63  MRKERRSGKGKHRSGRTCERSNLSTWFNYIESYKAYCDEYLTKISMEEFRPDKPLWEAHL 122

Query: 61  FKYPTSPTVAGTMVLKVHHSLGDGYSLMGVVLSCLHTASDLTFLSLFLPDVNDDSRTSVL 120
           FKYPTSPTVAGTMVLKVHHSLGDGYSLMGVVLSCLHTASDLTFLSLFLPDVNDDSRTSVL
Sbjct: 123 FKYPTSPTVAGTMVLKVHHSLGDGYSLMGVVLSCLHTASDLTFLSLFLPDVNDDSRTSVL 182

Query: 121 KGIDEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYM 180
           KGIDEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYM
Sbjct: 183 KGIDEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYM 242

Query: 181 EEMEKNSGEVAATALTIVNTRVIGTYRPATEMAKPESKGLW 221
           EEMEKNSGEVAATALTIVNTRVIGTYRPATEMAKPESKGLW
Sbjct: 243 EEMEKNSGEVAATALTIVNTRVIGTYRPATEMAKPESKGLW 283

[Close]

BLAST of CsGy7G004550 vs. ExPASy TrEMBL
Match: A0A5A7SKE3 (Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003010 PE=4 SV=1)

HSP 1 Score: 313 bits (801), Expect = 2.59e-102
Identity = 168/214 (78.50%), Postives = 173/214 (80.84%), Query Frame = 0


Query: 30  IESYKAYCDEYLTKISMEEFRPDKPLWEAHLFKYPTSPTVAGTMVLKVHHSLGDGYSLMG 89
           IESYKA+ DEYLTKISMEEFRPDKPLWE HLFKYPTSPTVAGTM+LK+HHSLGDGYSLMG
Sbjct: 92  IESYKAHFDEYLTKISMEEFRPDKPLWETHLFKYPTSPTVAGTMILKLHHSLGDGYSLMG 151

Query: 90  VVLSCLHTASD----LTFLSLFLPDVND------------------DSRTSVLKGIDEKT 149
           VVLSCLHTASD    LTF S      N+                  D   SV KGIDEKT
Sbjct: 152 VVLSCLHTASDPNVPLTFPSRRQRRFNNGVVKSLTGIVSAVCRTASDLGWSVFKGIDEKT 211

Query: 150 SIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYMEEMEKNS 209
            IRSGREGLQFMPMNTFTVSFSLN IKKIKTKL VTVNDVITGI FMGLRLYMEE+EKNS
Sbjct: 212 PIRSGREGLQFMPMNTFTVSFSLNDIKKIKTKLQVTVNDVITGIIFMGLRLYMEEIEKNS 271

Query: 210 GEVAATALTIVNTRVIGTYRPATEMAKPESKGLW 221
           GEVAATALTIVNTRVIG YR ATEMAKPESKGLW
Sbjct: 272 GEVAATALTIVNTRVIGNYRSATEMAKPESKGLW 305

[Close]

BLAST of CsGy7G004550 vs. ExPASy TrEMBL
Match: A0A1S3C1V4 (Diacylglycerol O-acyltransferase OS=Cucumis melo OX=3656 GN=LOC103495688 PE=4 SV=1)

HSP 1 Score: 313 bits (801), Expect = 2.59e-102
Identity = 168/214 (78.50%), Postives = 173/214 (80.84%), Query Frame = 0


Query: 30  IESYKAYCDEYLTKISMEEFRPDKPLWEAHLFKYPTSPTVAGTMVLKVHHSLGDGYSLMG 89
           IESYKA+ DEYLTKISMEEFRPDKPLWE HLFKYPTSPTVAGTM+LK+HHSLGDGYSLMG
Sbjct: 92  IESYKAHFDEYLTKISMEEFRPDKPLWETHLFKYPTSPTVAGTMILKLHHSLGDGYSLMG 151

Query: 90  VVLSCLHTASD----LTFLSLFLPDVND------------------DSRTSVLKGIDEKT 149
           VVLSCLHTASD    LTF S      N+                  D   SV KGIDEKT
Sbjct: 152 VVLSCLHTASDPNVPLTFPSRRQRRFNNGVVKSLTGIVSAVCRTASDLGWSVFKGIDEKT 211

Query: 150 SIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYMEEMEKNS 209
            IRSGREGLQFMPMNTFTVSFSLN IKKIKTKL VTVNDVITGI FMGLRLYMEE+EKNS
Sbjct: 212 PIRSGREGLQFMPMNTFTVSFSLNDIKKIKTKLQVTVNDVITGIIFMGLRLYMEEIEKNS 271

Query: 210 GEVAATALTIVNTRVIGTYRPATEMAKPESKGLW 221
           GEVAATALTIVNTRVIG YR ATEMAKPESKGLW
Sbjct: 272 GEVAATALTIVNTRVIGNYRSATEMAKPESKGLW 305

[Close]

BLAST of CsGy7G004550 vs. ExPASy TrEMBL
Match: A0A438JK81 (Diacylglycerol O-acyltransferase OS=Vitis vinifera OX=29760 GN=WSD1_5 PE=4 SV=1)

HSP 1 Score: 182 bits (463), Expect = 2.60e-51
Identity = 102/219 (46.58%), Postives = 133/219 (60.73%), Query Frame = 0


Query: 31  ESYKAYCDEYLTKISMEEFRPDKPLWEAHLFKYPTSPTVAGTMVLKVHHSLGDGYSLMGV 90
           ESY  Y D+YLTKI+M+EF   +PLWE H+ KYPTS   AGT+V K+HH+LGDG+SLMG 
Sbjct: 98  ESYDDYFDDYLTKIAMKEFPQSRPLWEIHIIKYPTS-NAAGTVVFKLHHALGDGFSLMGA 157

Query: 91  VLSCLHTASD----LTFLSLFLPDVNDDSRT---------------------SVLKGI-- 150
           +LSCL  A +    +TF SL      D  R+                     S+LK    
Sbjct: 158 LLSCLQRADNPNLPITFPSLKASSNLDKGRSIIRAVPRALSAAFNTVSDFGWSLLKSTVV 217

Query: 151 -DEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYMEE 210
            D++T IRSG  G++F P++  T+SFSL+YI+KIK KL VT+NDV+TGI F G RLYM+ 
Sbjct: 218 EDDRTPIRSGDPGVEFRPLSIATISFSLDYIQKIKAKLGVTINDVLTGIIFFGTRLYMQS 277

Query: 211 MEKNSGEVAATALTIVNTRVIGTYRPATEMAKPESKGLW 221
           M   S    +TAL ++NTRVI  Y+   EM   +S   W
Sbjct: 278 MNHASRNANSTALVLLNTRVISGYKSLKEMTASDSSSQW 315

[Close]

BLAST of CsGy7G004550 vs. ExPASy TrEMBL
Match: F6H5E6 (Diacylglycerol O-acyltransferase OS=Vitis vinifera OX=29760 GN=VIT_12s0028g03480 PE=4 SV=1)

HSP 1 Score: 182 bits (461), Expect = 3.18e-51
Identity = 101/220 (45.91%), Postives = 133/220 (60.45%), Query Frame = 0


Query: 31  ESYKAYCDEYLTKISMEEFRPDKPLWEAHLFKYPTSPTVAGTMVLKVHHSLGDGYSLMGV 90
           ESY  Y D+YLTKI+M+EF   +PLWE H+ KYPTS   AGT+V K+HH+LGDG+SLMG 
Sbjct: 98  ESYDDYFDDYLTKIAMKEFPQSRPLWEIHIIKYPTS-NAAGTVVFKLHHALGDGFSLMGA 157

Query: 91  VLSCLHTASD----LTFLSLFLPDVNDDSRTSVLKGI----------------------- 150
           +LSCL  A +    +TF S F    N DS  S+++ +                       
Sbjct: 158 LLSCLQRADNPNLPITFPS-FKVSSNLDSGRSIIRAVPRALSAAFNTVSDFGWGLLKSTA 217

Query: 151 --DEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYME 210
             D++T IRSG  G++F PM+  T+SFSL+ I+KIK KL VT+NDV+TGI F G RLYM+
Sbjct: 218 VEDDRTPIRSGDPGVEFRPMSIATISFSLDNIQKIKAKLGVTINDVLTGIIFFGTRLYMQ 277

Query: 211 EMEKNSGEVAATALTIVNTRVIGTYRPATEMAKPESKGLW 221
            M   S    +TAL ++NTRVI  Y+   EM   +S   W
Sbjct: 278 SMNHASRNANSTALVLLNTRVISGYKSIKEMTASDSSSQW 315

[Close]

BLAST of CsGy7G004550 vs. TAIR 10
Match: AT3G49190.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 75.1 bits (183), Expect = 7.9e-14
Identity = 65/242 (26.86%), Postives = 102/242 (42.15%), Query Frame = 0


Query: 30  IESYKAYCDEYLTKISMEEFRPDKPLWEAHLFKYPTSPTVAGTMVLKVHHSLGDGYSLMG 89
           IE+  A+ ++Y++ +        +PLWE HL    TS       VLK+HHS+GDG S+M 
Sbjct: 101 IENSNAFLEDYVSDLMKIPLDTSRPLWELHLLDLKTS-DAENVAVLKIHHSVGDGMSIMS 160

Query: 90  VVLSCLHTASDLTFL-------------SLFLPDVNDDSR---------TSVLKGI---- 149
           +VL+C+   S+   L             SL       DSR         T+V+ G+    
Sbjct: 161 LVLACMRKTSNPDELPSLPYQYRSSSRSSLLTTGSRSDSRLLWLVKVIWTAVILGLNTVC 220

Query: 150 -------------DEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITG 209
                        D +T I+      +   +     + SL+ IK  K  +++T+NDV+ G
Sbjct: 221 DALEFIVTTLFVKDTETPIKGDFLSTKSKQLRLVHRTVSLDDIKLTKNAMNMTINDVVLG 280

Query: 210 ITFMGL-----RLYMEEMEKNSGE------VAATALTIVNTRVIGTYRPATEMAKPESKG 222
           +T  GL     R Y EE  KN  +      +   +  +VN R     +   +M +  SK 
Sbjct: 281 VTQAGLSRYLARRYGEEETKNRKQKKLPKRIRLRSALLVNLRPTTGIQDLADMMEKGSKC 340

[Close]

BLAST of CsGy7G004550 vs. TAIR 10
Match: AT5G16350.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 72.8 bits (177), Expect = 3.9e-13
Identity = 62/244 (25.41%), Postives = 103/244 (42.21%), Query Frame = 0


Query: 31  ESYKAYCDEYLTKISMEEFRPDKPLWEAHLFKYPTSPTVAGTMVLKVHHSLGDGYSLMGV 90
           E  +++ D+Y++++++      +PLW+ H+    TS   A   V++ +H+L DG S + +
Sbjct: 88  EGGQSFVDDYISRLTLIPLDRSRPLWDIHILNVKTSYAEA-VGVIRFNHALADGMSFISL 147

Query: 91  VLSCLHTASDLTFLSLFLPDVNDDSRTS-------------------------------- 150
           VL+C H  S+   LS  +P V   S  S                                
Sbjct: 148 VLACTHKTSNPDMLSTAIPSVKRRSTVSHSLKKTGWFLTAIFTIGSTMRLIWNTLVDMFL 207

Query: 151 ----VLKGIDEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFM 210
               +L   D KT ++ G   ++  P   +  + SL+ IK IK  +++T+NDV+ GIT  
Sbjct: 208 LFATMLFLKDTKTPLKGG-ANVRSNPKTFYHRNISLDDIKLIKNAMNMTINDVLLGITQA 267

Query: 211 GLRLYME---EMEKNSGE--------------VAATALTIVNTRVIGTYRPATEMAKPES 222
            L  Y+    E E N+ E              +   A   VN R    ++P  EM   +S
Sbjct: 268 ALSSYLNRRYEHENNNEEDGVLTSYTNNLPDRIRFRAGCTVNLRSDIGFKPLAEMMVKDS 327

[Close]

BLAST of CsGy7G004550 vs. TAIR 10
Match: AT5G12420.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 72.8 bits (177), Expect = 3.9e-13
Identity = 61/242 (25.21%), Postives = 102/242 (42.15%), Query Frame = 0


Query: 31  ESYKAYCDEYLTKISMEEFRPDKPLWEAHLFKYPTSPTVAGTMVLKVHHSLGDGYSLMGV 90
           E  +++ D+Y+++++M      +PLW+ H+    TS   A    ++ HHSLGDG SL+ +
Sbjct: 88  EGGQSFIDDYMSRLTMIPLDRSRPLWDIHILNVKTSEAEA-VGFIRSHHSLGDGMSLISL 147

Query: 91  VLSCLHTASDLTFLSLFLPDVNDDSRTS-------------------------------- 150
           +L+C H  SD    S  +P +   +  S                                
Sbjct: 148 MLACTHKTSDPDMFSNAIPSMKRRATMSHSLKTKGWFLRSIFTIGSTMRLLWNTTIDMLL 207

Query: 151 ----VLKGIDEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFM 210
               VL   D KT +++G + ++  P   +    SL+ IK IK  +++T+NDV+ GIT  
Sbjct: 208 LLATVLFLKDTKTPLKAGAD-VRSNPKRFYHRIISLDDIKLIKNAMNMTINDVLFGITQA 267

Query: 211 GLRLYMEEM--EKNSGEVAATA-------------LTIVNTRVIGTYRPATEMAKPESKG 222
            L  Y+      K   + A T+                VN R    ++P  +M   +SK 
Sbjct: 268 SLSHYLNRQYGTKKEEDGALTSYRNNLPDGIRFRVACTVNLRSDIGFKPLADMMVKDSKC 327

[Close]

BLAST of CsGy7G004550 vs. TAIR 10
Match: AT5G53390.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 69.7 bits (169), Expect = 3.3e-12
Identity = 62/236 (26.27%), Postives = 96/236 (40.68%), Query Frame = 0


Query: 30  IESYKAYCDEYLTKISMEEFRPDKPLWEAHLFKYPTSPTVAGTMVLKVHHSLGDGYSLMG 89
           IE+   Y ++Y++K++       KPLWE HL    TS       +LK+HHSLGDG SLM 
Sbjct: 96  IENPDQYLEDYISKLTTIPMDLSKPLWEMHLLGVKTS-NAESYAILKIHHSLGDGMSLMS 155

Query: 90  VVLSCLHTASDLTFL-------SLFLPDVNDDSRTSV---LKGI---------------- 149
           ++L+C    SD   L         F P  N      +     G+                
Sbjct: 156 LLLACTRKTSDPEALPTVAVHKKRFGPSCNSGFFNKIWWLFVGLWFILRLLFNTFVDILM 215

Query: 150 ---------DEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFM 209
                    D +T + + + G + +P        S + +K +K  + +TVNDV+ G+T  
Sbjct: 216 FALTIFVLRDTETPLLA-KPGSELIPKRFVHRIISFDDVKLVKNAMKMTVNDVLLGVTQA 275

Query: 210 GL-----RLYMEEMEKNSGE----VAATALTIVNTRVIGTYRPATEMAKPESKGLW 222
           GL     R Y +E    S E    +   +  ++N R         +M   +SK  W
Sbjct: 276 GLSRYLSRKYDQEATPKSKESMRRIRLRSAIMINLRPNAGIEALADMMAKKSKCRW 329

[Close]

BLAST of CsGy7G004550 vs. TAIR 10
Match: AT3G49200.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 66.2 bits (160), Expect = 3.7e-11
Identity = 64/245 (26.12%), Postives = 98/245 (40.00%), Query Frame = 0


Query: 29  YIESYKA--YCDEYLTKISMEEFRPDKPLWEAHLFKYPTSPTVAGTMVLKVHHSLGDGYS 88
           +IE+  A  + + Y++ ++       KPLWE HL    TS       VL++HHSLGDG S
Sbjct: 110 HIENANADVFLESYVSDLTTIPLDTSKPLWEVHLLDLKTS-DAENVAVLRIHHSLGDGMS 169

Query: 89  LMGVVLSCLHTASDLTFLSLFLPDVNDDSRTSVLKGIDEKTS----------------IR 148
           +M +VL+C    S+   L          S +S LK      S                + 
Sbjct: 170 MMSLVLACTRKTSNPNELPSLPYQNRPSSGSSSLKTSSRCYSRFFWLVMVLWSAALLVLN 229

Query: 149 SGREGLQFMPMNTFTV-----------------------SFSLNYIKKIKTKLDVTVNDV 208
           +  + L+F+    F                         + SL+ IK IK  + +TVNDV
Sbjct: 230 TVCDALEFIATALFLKDTETPIKGDFKLSKGKRMCMVHRTVSLDDIKLIKNAMKMTVNDV 289

Query: 209 ITGITFMGLRLYM-------EEMEKNSGE----VAATALTIVNTRVIGTYRPATEMAKPE 222
           + G++  GL  Y+       EE ++NS      +   A  +VN R     +   +M    
Sbjct: 290 VLGVSQAGLSQYLKRRYGEQEESKRNSSNIPKGIRLRAALLVNLRPTTGIQDLADMMTKG 349

The following BLAST results are available for this feature:

BLAST of CsGy7G004550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M3B3	1.1e-12	26.86	Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3...	[more]
Q94CK0	5.5e-12	25.21	Wax ester synthase/diacylglycerol acyltransferase 7 OS=Arabidopsis thaliana OX=3...	[more]
Q9FFE8	5.5e-12	25.41	Wax ester synthase/diacylglycerol acyltransferase 8 OS=Arabidopsis thaliana OX=3...	[more]
Q5KS41	4.7e-11	26.27	Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=...	[more]
Q9M3B2	5.2e-10	26.12	Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of CsGy7G004550 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008455536.1	5.35e-102	78.50	PREDICTED: O-acyltransferase WSD1-like [Cucumis melo] >KAA0031532.1 O-acyltransf...	[more]
KAE8645787.1	9.06e-80	100.00	hypothetical protein Csa_017378, partial [Cucumis sativus]	[more]
XP_031260551.1	2.10e-56	46.85	LOW QUALITY PROTEIN: O-acyltransferase WSD1-like [Pistacia vera]	[more]
XP_031280043.1	1.66e-54	45.95	O-acyltransferase WSD1-like [Pistacia vera]	[more]
XP_031280304.1	2.37e-51	44.34	O-acyltransferase WSD1-like [Pistacia vera]	[more]

BLAST of CsGy7G004550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K3V2	4.44e-157	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G051340 PE=4 SV=1	[more]
A0A5A7SKE3	2.59e-102	78.50	Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3C1V4	2.59e-102	78.50	Diacylglycerol O-acyltransferase OS=Cucumis melo OX=3656 GN=LOC103495688 PE=4 SV...	[more]
A0A438JK81	2.60e-51	46.58	Diacylglycerol O-acyltransferase OS=Vitis vinifera OX=29760 GN=WSD1_5 PE=4 SV=1	[more]
F6H5E6	3.18e-51	45.91	Diacylglycerol O-acyltransferase OS=Vitis vinifera OX=29760 GN=VIT_12s0028g03480...	[more]

BLAST of CsGy7G004550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G49190.1	7.9e-14	26.86	O-acyltransferase (WSD1-like) family protein	[more]
AT5G16350.1	3.9e-13	25.41	O-acyltransferase (WSD1-like) family protein	[more]
AT5G12420.1	3.9e-13	25.21	O-acyltransferase (WSD1-like) family protein	[more]
AT5G53390.1	3.3e-12	26.27	O-acyltransferase (WSD1-like) family protein	[more]
AT3G49200.1	3.7e-11	26.12	O-acyltransferase (WSD1-like) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR31650:SF56	SUBFAMILY NOT NAMED	coord: 2..214
IPR045034	O-acyltransferase WSD1-like	PANTHER	PTHR31650	O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN	coord: 2..214

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy7G004550.2	CsGy7G004550.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019432	triglyceride biosynthetic process
biological_process	GO:0045017	glycerolipid biosynthetic process
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0004144	diacylglycerol O-acyltransferase activity
molecular_function	GO:0008374	O-acyltransferase activity