CsGy7G004020 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy7G004020
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionLipoyl synthase, mitochondrial
LocationGy14Chr7: 3034607 .. 3036789 (+)
RNA-Seq ExpressionCsGy7G004020
SyntenyCsGy7G004020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACAAGCGCTGCCAAAGCCTTGCTCGAATCCTCAAATCCGTCAGTAGATCATTCTCTTCTTCATCTTCTTCCACCTCCGCCTCTGCTACATCCTCCCAATTCCCCCGCACGTTGGCCGGTCTCCGGGAACGACTCGCCGCGGAGTCCCCAATACTTTCTGATTTCGTTGATCTGCAATCTTCTGATTCGTATTCTGTGGAGGTTGGTACGAAGAAGAAGCCCCTTCCTAAACCTAAATGGATGAGGGAATCGGTGCCTGGAGGCGAGAAGTATGTTCAGATAAAGAAGAAGCTCCGGGAATTGAAGCTTCACACGGTTTGTGAGGAGGCTAAATGCCCTAATTTAGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACAATTATGATTCTTGGTGACACTTGTACTCGTGGTTGCAGGTTAGTAGTAATTCAGTTTATTCACTCCATTTCGTTATGCTTATTTAATTGCCAATTAGCGACTGAAGAACCATGATTTCTTGTTATTTTCCCGTGTGTAATGTCAACGTATATATAATTTAGTGGGGAACGTGAATGGACTTAGAACTGATTGTTTTTGTCATAGAAAATGACCCTGTCGATTACTTTGATATCATCGGGGTGCCTCAGAATTTCTAATTCCATTGCTCTTGTGGGATTGTTTGAACTCCAGGTTCTGTAACGTGAAGACATCACGAACTCCACCTCCACCGGATCCAAATGAGCCCACAAATGTTGCTGAAGCAATTGCATCATGGGGTTTGGATTATGTGGTCATTACCAGCGTTGATCGCGACGATTTACCTGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGCAGAAACTTAAGGTGTTGAAGCCAAATATGCTCATTGAGGCCTTGGGTACTAATTCCCTAGTTTCTATTACGCATAACAGTGTCTAGTTCATTGATGCATTCAAGGATCCTTGATTTAAGGAACCTTATTTTATTTGGTTTCGATGCAACTAAGAAATTTAGTGAAAGCTACTAATTTTACCCTGTACTATTGACGTCGCCAAAATCAGCATATGATATTGATTTGCTTCTGACTATGTTTGTCCAGTGTGATTTTATCTCTTCTTTGATGTTGCTTATGCTTAACTAATTGATTCTGCAGTTCCTGATTTTCGAGGAGACTCTGGCTGTGTGGAGAAAGTTGCAAGGTCGGGCCTTGACGTCTTTGCTCACAATATAGAGACAGTTGAAGAGCTTCAAGGAGTAGTTAGGGATCACCGTGCTAATTTCAAGCAATCATTAGATGTTCTAGTTATGGCCAAAGAGTTTTCTCTATCTGGAACTCTTACAAAGACTTCCATTATGTTAGGCTGTGGGGAAACACCAGATCAAGTTGTTCAAACAATGGAGAAGGTGAGAGCAGCTGGAGTTGATGTGATGACATTTGGGCAGTATATGAGACCTTCAAAACGCCACATGCCTGTATCAGAATACATTACTCCTGAGGCTTTTGAGAAGTATCAAAATATTGGCATGAAAATGGTACTTTCTCTTTCTAATTATTTGAACATTTTGATTGTATACTGGCTTCCCTGTTCTGCTAGTGAAAAATGATGATTGCTCAATCAATACCTTAAAGGCAAGTCGACTTGATTTTTTTATAAAAAAAAGTTATAATATTGTATCATTTCCCATCAGGCTAGGATGAATCAAAGTGCATAAGTTTTTCTCTAACTTAAATATCTATTTTAAGCACACTTCAAAAGTTAATCCAAACAGGTTTTATTGGATTCATTCAGTGTGAATAACTTTCATTTGCATTTTCCTTCTCTCGGATCTGTACCGTTGTAATTTTTCGTGTGTTGATTTGTTAGAGGGAGTCCATGTTTTACCTAAAGCATATACTAATTCACACTTCGATTCATAACCCTCATTTTTAAGTTTATAGGAAATCTCCATCATCAAGTAGTACTTATTATATGATCAATAGTCTTTTAATGGCTGGAGTTACCTGCCAGCCTTGAGGGTGCAACAATTATCTAAAAATCTTTTTTTTGTTTGGTTGTGGCAGGGTTTTCGATATGTGGCTTCTGGCCCAATGGTTAGGTCTTCATACAAGGCAGGAGAATTCTATATCAAGTCAATGATTGAATCAGATCGGGCAGCTTCTAATTCACAGCCTACCCAACTTTGA

mRNA sequence

ATGAACAAGCGCTGCCAAAGCCTTGCTCGAATCCTCAAATCCGTCAGTAGATCATTCTCTTCTTCATCTTCTTCCACCTCCGCCTCTGCTACATCCTCCCAATTCCCCCGCACGTTGGCCGGTCTCCGGGAACGACTCGCCGCGGAGTCCCCAATACTTTCTGATTTCGTTGATCTGCAATCTTCTGATTCGTATTCTGTGGAGGTTGGTACGAAGAAGAAGCCCCTTCCTAAACCTAAATGGATGAGGGAATCGGTGCCTGGAGGCGAGAAGTATGTTCAGATAAAGAAGAAGCTCCGGGAATTGAAGCTTCACACGGTTTGTGAGGAGGCTAAATGCCCTAATTTAGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACAATTATGATTCTTGGTGACACTTGTACTCGTGGTTGCAGGTTCTGTAACGTGAAGACATCACGAACTCCACCTCCACCGGATCCAAATGAGCCCACAAATGTTGCTGAAGCAATTGCATCATGGGGTTTGGATTATGTGGTCATTACCAGCGTTGATCGCGACGATTTACCTGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGCAGAAACTTAAGGTGTTGAAGCCAAATATGCTCATTGAGGCCTTGGTTCCTGATTTTCGAGGAGACTCTGGCTGTGTGGAGAAAGTTGCAAGGTCGGGCCTTGACGTCTTTGCTCACAATATAGAGACAGTTGAAGAGCTTCAAGGAGTAGTTAGGGATCACCGTGCTAATTTCAAGCAATCATTAGATGTTCTAGTTATGGCCAAAGAGTTTTCTCTATCTGGAACTCTTACAAAGACTTCCATTATGTTAGGCTGTGGGGAAACACCAGATCAAGTTGTTCAAACAATGGAGAAGGTGAGAGCAGCTGGAGTTGATGTGATGACATTTGGGCAGTATATGAGACCTTCAAAACGCCACATGCCTGTATCAGAATACATTACTCCTGAGGCTTTTGAGAAGTATCAAAATATTGGCATGAAAATGGGTTTTCGATATGTGGCTTCTGGCCCAATGGTTAGGTCTTCATACAAGGCAGGAGAATTCTATATCAAGTCAATGATTGAATCAGATCGGGCAGCTTCTAATTCACAGCCTACCCAACTTTGA

Coding sequence (CDS)

ATGAACAAGCGCTGCCAAAGCCTTGCTCGAATCCTCAAATCCGTCAGTAGATCATTCTCTTCTTCATCTTCTTCCACCTCCGCCTCTGCTACATCCTCCCAATTCCCCCGCACGTTGGCCGGTCTCCGGGAACGACTCGCCGCGGAGTCCCCAATACTTTCTGATTTCGTTGATCTGCAATCTTCTGATTCGTATTCTGTGGAGGTTGGTACGAAGAAGAAGCCCCTTCCTAAACCTAAATGGATGAGGGAATCGGTGCCTGGAGGCGAGAAGTATGTTCAGATAAAGAAGAAGCTCCGGGAATTGAAGCTTCACACGGTTTGTGAGGAGGCTAAATGCCCTAATTTAGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACAATTATGATTCTTGGTGACACTTGTACTCGTGGTTGCAGGTTCTGTAACGTGAAGACATCACGAACTCCACCTCCACCGGATCCAAATGAGCCCACAAATGTTGCTGAAGCAATTGCATCATGGGGTTTGGATTATGTGGTCATTACCAGCGTTGATCGCGACGATTTACCTGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGCAGAAACTTAAGGTGTTGAAGCCAAATATGCTCATTGAGGCCTTGGTTCCTGATTTTCGAGGAGACTCTGGCTGTGTGGAGAAAGTTGCAAGGTCGGGCCTTGACGTCTTTGCTCACAATATAGAGACAGTTGAAGAGCTTCAAGGAGTAGTTAGGGATCACCGTGCTAATTTCAAGCAATCATTAGATGTTCTAGTTATGGCCAAAGAGTTTTCTCTATCTGGAACTCTTACAAAGACTTCCATTATGTTAGGCTGTGGGGAAACACCAGATCAAGTTGTTCAAACAATGGAGAAGGTGAGAGCAGCTGGAGTTGATGTGATGACATTTGGGCAGTATATGAGACCTTCAAAACGCCACATGCCTGTATCAGAATACATTACTCCTGAGGCTTTTGAGAAGTATCAAAATATTGGCATGAAAATGGGTTTTCGATATGTGGCTTCTGGCCCAATGGTTAGGTCTTCATACAAGGCAGGAGAATTCTATATCAAGTCAATGATTGAATCAGATCGGGCAGCTTCTAATTCACAGCCTACCCAACTTTGA

Protein sequence

MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQSSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNIETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAASNSQPTQL*
Homology
BLAST of CsGy7G004020 vs. ExPASy Swiss-Prot
Match: B9H5L9 (Lipoyl synthase, mitochondrial OS=Populus trichocarpa OX=3694 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 4.8e-181
Identity = 315/366 (86.07%), Postives = 340/366 (92.90%), Query Frame = 0

Query: 13  KSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQSSDSYSVEVGTK 72
           K+ +R+FSSS+   S++    QF +TLAGLR RLA ESP LSDF+ LQS+++YSVEVGTK
Sbjct: 19  KAKNRTFSSSTVE-SSTKQPPQFSQTLAGLRARLAVESPTLSDFIHLQSNNTYSVEVGTK 78

Query: 73  KKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATI 132
           KKPLPKPKWMRE++PGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATI
Sbjct: 79  KKPLPKPKWMREAIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATI 138

Query: 133 MILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGS 192
           MILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDL DQGS
Sbjct: 139 MILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLADQGS 198

Query: 193 GHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNIETVEELQGVVRD 252
           GHFAETV KLK LKPNMLIEALVPDFRGD GCVEKVA+SGLDVFAHNIETVEELQ  VRD
Sbjct: 199 GHFAETVHKLKTLKPNMLIEALVPDFRGDRGCVEKVAKSGLDVFAHNIETVEELQSSVRD 258

Query: 253 HRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQY 312
           HRANFKQSLDVL+MAKE++  GTLTKTSIMLGCGE P+QVV+TMEKVRAAGVDVMTFGQY
Sbjct: 259 HRANFKQSLDVLMMAKEYAPPGTLTKTSIMLGCGEAPEQVVKTMEKVRAAGVDVMTFGQY 318

Query: 313 MRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRA 372
           MRPSKRHMPVSEYITP+AFEKY+ +GM+MGFRYVASGPMVRSSYKAGEFYIKSMIESDR+
Sbjct: 319 MRPSKRHMPVSEYITPDAFEKYKTLGMEMGFRYVASGPMVRSSYKAGEFYIKSMIESDRS 378

Query: 373 ASNSQP 379
            S+  P
Sbjct: 379 VSSQLP 383

BLAST of CsGy7G004020 vs. ExPASy Swiss-Prot
Match: Q3LSN5 (Lipoyl synthase 2, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-2 PE=2 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 4.1e-180
Identity = 311/375 (82.93%), Postives = 346/375 (92.27%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60
           M  R +++A  L   ++  SSSS++T+ ++  S+  + LA LR RLA ESP LSDF+ L+
Sbjct: 2   MYSRFRTVAGNLNCAAKRLSSSSTTTTTTSAPSELQQNLAALRARLAMESPSLSDFISLK 61

Query: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S ++YSVEVGTKKKPLPKPKWM+ES+PGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 62  SDNAYSVEVGTKKKPLPKPKWMKESIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 121

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDP+EPTNVAEAIASWGLDYVVIT
Sbjct: 122 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVIT 181

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHF ETVQKLK LKP+MLIEALVPDFRG++ CVEKV++SGLDVFAHNI
Sbjct: 182 SVDRDDLPDQGSGHFTETVQKLKALKPSMLIEALVPDFRGNAECVEKVSKSGLDVFAHNI 241

Query: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ  VRDHRANFKQSLDVL+MAKE++ +GTLTKTSIMLGCGETPDQ+V+TMEKVR
Sbjct: 242 ETVEELQSAVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLGCGETPDQIVKTMEKVR 301

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQ+MRPSKRHMPVSEYITPEAFEKYQ +GM+MGFRYVASGPMVRSSYKAGE
Sbjct: 302 AAGVDVMTFGQHMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGE 361

Query: 361 FYIKSMIESDRAASN 376
           FYIKSMI+SDRAAS+
Sbjct: 362 FYIKSMIDSDRAASS 376

BLAST of CsGy7G004020 vs. ExPASy Swiss-Prot
Match: Q3LSN4 (Lipoyl synthase 1, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-1 PE=2 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 5.3e-180
Identity = 311/375 (82.93%), Postives = 345/375 (92.00%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60
           M  R +++A  L   ++  SSSS++T+ ++  S+  + LA LR RLA ESP LSDF+ L+
Sbjct: 2   MYSRFRTVAGNLNCAAKRLSSSSTTTTTTSAPSELQQNLAALRARLAMESPSLSDFISLK 61

Query: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S ++YSVEVGTKKKPLPKPKWM+ES+PGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 62  SDNAYSVEVGTKKKPLPKPKWMKESIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 121

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDP+EPTNVAEAIASWGLDYVVIT
Sbjct: 122 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVIT 181

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHF ETVQKLK LKP+ LIEALVPDFRG++ CVEKV++SGLDVFAHNI
Sbjct: 182 SVDRDDLPDQGSGHFTETVQKLKALKPSTLIEALVPDFRGNAECVEKVSKSGLDVFAHNI 241

Query: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ  VRDHRANFKQSLDVL+MAKE++ +GTLTKTSIMLGCGETPDQ+V+TMEKVR
Sbjct: 242 ETVEELQSAVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLGCGETPDQIVKTMEKVR 301

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQ +GM+MGFRYVASGPMVRSSYKAGE
Sbjct: 302 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGE 361

Query: 361 FYIKSMIESDRAASN 376
           FYIKSMI+SDRAAS+
Sbjct: 362 FYIKSMIDSDRAASS 376

BLAST of CsGy7G004020 vs. ExPASy Swiss-Prot
Match: B7FM45 (Lipoyl synthase, mitochondrial OS=Medicago truncatula OX=3880 GN=LIP1 PE=2 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 5.0e-178
Identity = 305/375 (81.33%), Postives = 346/375 (92.27%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60
           M  R +++A+ LKS ++ FS ++++T+ + +SS+FP+ L  LR RLA ESP LSDF+ L+
Sbjct: 2   MYSRFRTVAKNLKSTTKPFSFTTATTTTTVSSSEFPQNLTELRARLARESPSLSDFISLK 61

Query: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S+++YSVEVGTKK PLPKPKWM+ES+PGG KYVQIKKKLRELKLHTVCEEAKCPN+GECW
Sbjct: 62  SNNAYSVEVGTKKNPLPKPKWMKESIPGGWKYVQIKKKLRELKLHTVCEEAKCPNMGECW 121

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDP+EPTNVAEAIASWGLDYVVIT
Sbjct: 122 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVIT 181

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SV RDDLPDQGS HF ETVQKLK+LKP++LIEALVPDFRG++ CVEKV++SGLDVFAHNI
Sbjct: 182 SVGRDDLPDQGSSHFTETVQKLKILKPSILIEALVPDFRGNAECVEKVSKSGLDVFAHNI 241

Query: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ  VRDHRANF QSLDVL MAK+++ +GTLTKTSIMLGCGETPDQ+V+TMEKVR
Sbjct: 242 ETVEELQSAVRDHRANFNQSLDVLRMAKDYAPAGTLTKTSIMLGCGETPDQIVKTMEKVR 301

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQ +GM+MGFRYVASGPMVRSSYKAGE
Sbjct: 302 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGE 361

Query: 361 FYIKSMIESDRAASN 376
           FYIKSMI+SDRA S+
Sbjct: 362 FYIKSMIDSDRAVSS 376

BLAST of CsGy7G004020 vs. ExPASy Swiss-Prot
Match: A5CB81 (Lipoyl synthase, mitochondrial OS=Vitis vinifera OX=29760 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 6.1e-176
Identity = 303/357 (84.87%), Postives = 330/357 (92.44%), Query Frame = 0

Query: 27  SASATSSQFPRTLAGLRERLAAESPILSDFVDLQSSDSYSVEVGTKKKPLPKPKWMRESV 86
           S+  T+ + P+TLA LR RLA ESP LSDFV LQ+SD YSVEVGTKKKPL KPKWM+ES+
Sbjct: 34  SSPPTNPEPPQTLASLRHRLAVESPSLSDFVRLQTSDDYSVEVGTKKKPLSKPKWMKESI 93

Query: 87  PGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFC 146
           PGG KY QIKKKLR+L LHTVCEEA+CPN+GECWSGGETGTATATIMILGDTCTRGCRFC
Sbjct: 94  PGGAKYTQIKKKLRQLNLHTVCEEARCPNMGECWSGGETGTATATIMILGDTCTRGCRFC 153

Query: 147 NVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLK 206
           NVKTSRTPPPPDP+EP+ VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLK+LK
Sbjct: 154 NVKTSRTPPPPDPDEPSKVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKILK 213

Query: 207 PNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNIETVEELQGVVRDHRANFKQSLDVLVM 266
           PNMLIEALVPDFRGD GCVEKV++SGLDVFAHNIETVEELQ  VRDHRANFKQSL+VL +
Sbjct: 214 PNMLIEALVPDFRGDPGCVEKVSKSGLDVFAHNIETVEELQSAVRDHRANFKQSLEVLKL 273

Query: 267 AKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYI 326
           AKE++ +GTLTKTSIMLGCGETPDQVV+TMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYI
Sbjct: 274 AKEYADAGTLTKTSIMLGCGETPDQVVRTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYI 333

Query: 327 TPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAAS--NSQPTQL 382
           TPEAFEKY+ +GM MGFRYVASGPMVRSSYKAGEFYIKSMI++DRA S  +S P+ L
Sbjct: 334 TPEAFEKYRILGMDMGFRYVASGPMVRSSYKAGEFYIKSMIDADRAMSWASSSPSPL 390

BLAST of CsGy7G004020 vs. NCBI nr
Match: XP_004136815.1 (lipoyl synthase 2, mitochondrial [Cucumis sativus] >KGN43590.1 hypothetical protein Csa_020197 [Cucumis sativus])

HSP 1 Score: 749 bits (1934), Expect = 1.26e-272
Identity = 381/381 (100.00%), Postives = 381/381 (100.00%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60
           MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQL 381
           FYIKSMIESDRAASNSQPTQL
Sbjct: 361 FYIKSMIESDRAASNSQPTQL 381

BLAST of CsGy7G004020 vs. NCBI nr
Match: XP_016901725.1 (PREDICTED: lipoyl synthase 2, mitochondrial isoform X1 [Cucumis melo] >KAA0031592.1 lipoyl synthase 2 [Cucumis melo var. makuwa] >TYK07044.1 lipoyl synthase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 741 bits (1912), Expect = 2.85e-269
Identity = 376/381 (98.69%), Postives = 378/381 (99.21%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60
           MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESP LSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWM+ESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQG VRDHRANFKQSLDVLVMAKEFS SGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQN+GMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQL 381
           FYIKSMIESDRAASNSQPTQL
Sbjct: 361 FYIKSMIESDRAASNSQPTQL 381

BLAST of CsGy7G004020 vs. NCBI nr
Match: XP_038887854.1 (lipoyl synthase 2, mitochondrial [Benincasa hispida])

HSP 1 Score: 731 bits (1886), Expect = 2.62e-265
Identity = 369/380 (97.11%), Postives = 375/380 (98.68%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60
           MNKRCQSL RILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESP LSDFVDLQ
Sbjct: 1   MNKRCQSLTRILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWM+ESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETV KLKVLKPNMLIEALVPDFRGD+GCVEKVA+SGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVHKLKVLKPNMLIEALVPDFRGDTGCVEKVAKSGLDVFAHNI 240

Query: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQG VRDHRANFKQSLDVLVMAKEF+ SGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQN+GMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQ 380
           FYIKSMIESDRAASNSQPT+
Sbjct: 361 FYIKSMIESDRAASNSQPTR 380

BLAST of CsGy7G004020 vs. NCBI nr
Match: XP_022147922.1 (lipoyl synthase 2, mitochondrial [Momordica charantia])

HSP 1 Score: 705 bits (1819), Expect = 4.25e-255
Identity = 357/380 (93.95%), Postives = 364/380 (95.79%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60
           M   CQSL RIL+S  RSFSSSSSSTSASATSSQFPRTLA LRERLAAESP LSDFV LQ
Sbjct: 1   MKTPCQSLVRILESFGRSFSSSSSSTSASATSSQFPRTLAALRERLAAESPSLSDFVGLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S+DSYSVEVGTKKKPLPKPKWM+ESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  STDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQ LKVLKPNMLIEALVPDFRGD GCVEKVA+SGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQTLKVLKPNMLIEALVPDFRGDIGCVEKVAKSGLDVFAHNI 240

Query: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQG VRDHRANFKQSLDVL MAK+F+ SGTLTKTSIMLGCGE PDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLAMAKDFAPSGTLTKTSIMLGCGEKPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQN+GMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQ 380
           FYIKSMIESDRAASNSQPTQ
Sbjct: 361 FYIKSMIESDRAASNSQPTQ 380

BLAST of CsGy7G004020 vs. NCBI nr
Match: XP_023531015.1 (lipoyl synthase 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 701 bits (1808), Expect = 2.02e-253
Identity = 357/380 (93.95%), Postives = 363/380 (95.53%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60
           MN R  SLARI +SV RS S SSSSTSASATSSQ PRTLAGLRERLAAESP LSDFV L+
Sbjct: 1   MNTRRHSLARIFESVGRSLSYSSSSTSASATSSQIPRTLAGLRERLAAESPTLSDFVGLK 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWM+ESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPQNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGD G VEKVA+SGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGSVEKVAKSGLDVFAHNI 240

Query: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQG VRDHRANFKQSLDVLVMAKEF+ SGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKY N+GMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQ 380
           FYIKSMIESDRAASNS PTQ
Sbjct: 361 FYIKSMIESDRAASNSHPTQ 380

BLAST of CsGy7G004020 vs. ExPASy TrEMBL
Match: A0A0A0K1R3 (Lipoyl synthase, mitochondrial OS=Cucumis sativus OX=3659 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 749 bits (1934), Expect = 6.12e-273
Identity = 381/381 (100.00%), Postives = 381/381 (100.00%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60
           MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQL 381
           FYIKSMIESDRAASNSQPTQL
Sbjct: 361 FYIKSMIESDRAASNSQPTQL 381

BLAST of CsGy7G004020 vs. ExPASy TrEMBL
Match: A0A5A7SKJ1 (Lipoyl synthase, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 741 bits (1912), Expect = 1.38e-269
Identity = 376/381 (98.69%), Postives = 378/381 (99.21%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60
           MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESP LSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWM+ESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQG VRDHRANFKQSLDVLVMAKEFS SGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQN+GMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQL 381
           FYIKSMIESDRAASNSQPTQL
Sbjct: 361 FYIKSMIESDRAASNSQPTQL 381

BLAST of CsGy7G004020 vs. ExPASy TrEMBL
Match: A0A1S4E162 (Lipoyl synthase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495529 PE=3 SV=1)

HSP 1 Score: 741 bits (1912), Expect = 1.38e-269
Identity = 376/381 (98.69%), Postives = 378/381 (99.21%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60
           MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESP LSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWM+ESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQG VRDHRANFKQSLDVLVMAKEFS SGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQN+GMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQL 381
           FYIKSMIESDRAASNSQPTQL
Sbjct: 361 FYIKSMIESDRAASNSQPTQL 381

BLAST of CsGy7G004020 vs. ExPASy TrEMBL
Match: A0A6J1D3N5 (Lipoyl synthase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111016734 PE=3 SV=1)

HSP 1 Score: 705 bits (1819), Expect = 2.06e-255
Identity = 357/380 (93.95%), Postives = 364/380 (95.79%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60
           M   CQSL RIL+S  RSFSSSSSSTSASATSSQFPRTLA LRERLAAESP LSDFV LQ
Sbjct: 1   MKTPCQSLVRILESFGRSFSSSSSSTSASATSSQFPRTLAALRERLAAESPSLSDFVGLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S+DSYSVEVGTKKKPLPKPKWM+ESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  STDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQ LKVLKPNMLIEALVPDFRGD GCVEKVA+SGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQTLKVLKPNMLIEALVPDFRGDIGCVEKVAKSGLDVFAHNI 240

Query: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQG VRDHRANFKQSLDVL MAK+F+ SGTLTKTSIMLGCGE PDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLAMAKDFAPSGTLTKTSIMLGCGEKPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQN+GMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQ 380
           FYIKSMIESDRAASNSQPTQ
Sbjct: 361 FYIKSMIESDRAASNSQPTQ 380

BLAST of CsGy7G004020 vs. ExPASy TrEMBL
Match: A0A6J1ERM2 (Lipoyl synthase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111437162 PE=3 SV=1)

HSP 1 Score: 698 bits (1802), Expect = 8.02e-253
Identity = 356/380 (93.68%), Postives = 363/380 (95.53%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60
           MN R  SLARI +SV RS S SSSSTSASATSSQ PRTLAGLRERLAAESP LSDFV L+
Sbjct: 1   MNARRHSLARIFESVGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVGLK 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWM+ESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPQNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGD G VEKVA+SGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGSVEKVAKSGLDVFAHNI 240

Query: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQG VRDHRANFKQSLDVLVMAKEF+ SGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKY N+GMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQ 380
           FYIKSMIESDRAAS+S PTQ
Sbjct: 361 FYIKSMIESDRAASSSHPTQ 380

BLAST of CsGy7G004020 vs. TAIR 10
Match: AT2G20860.1 (lipoic acid synthase 1 )

HSP 1 Score: 616.7 bits (1589), Expect = 1.3e-176
Identity = 307/376 (81.65%), Postives = 338/376 (89.89%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60
           M+ R   L R L+  SR FSSSS+ T  + T S  P++L  LR RLA ESP L+DF+   
Sbjct: 1   MHSRSALLYRFLRPASRCFSSSSAVTPVTVTQS--PKSLEALRARLANESPSLTDFI--- 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
             D+YSVEVGTKKKPLPKPKWM+ES+PGGE+YVQIKKKLR+LKLHTVCEEAKCPNLGECW
Sbjct: 61  HGDTYSVEVGTKKKPLPKPKWMKESIPGGERYVQIKKKLRDLKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWG+DYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPNNVAEAIASWGVDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQ+LK LKP MLIEALVPDFRGD GCVEKV++SGLDV AHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQRLKFLKPEMLIEALVPDFRGDGGCVEKVSKSGLDVLAHNI 240

Query: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ  VRDHRANFKQSLDVL MAKE++ +GTLTKTS+MLGCGETPDQVV+TMEKVR
Sbjct: 241 ETVEELQSFVRDHRANFKQSLDVLRMAKEYAPAGTLTKTSVMLGCGETPDQVVKTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPV+EY+TP+AFE+Y+ +GM+MGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVAEYVTPDAFERYRLLGMEMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNS 377
           +YIKSMIE+DR AS S
Sbjct: 361 YYIKSMIEADRVASPS 371

BLAST of CsGy7G004020 vs. TAIR 10
Match: AT5G08415.1 (Radical SAM superfamily protein )

HSP 1 Score: 378.3 bits (970), Expect = 7.5e-105
Identity = 198/385 (51.43%), Postives = 262/385 (68.05%), Query Frame = 0

Query: 18  SFSSSSSSTSASATSSQFPRTLAGLR-ERLAAESPILSDFVDLQS--SDSYSVE------ 77
           +FS S S+      SS+F      +R E     SP+ +  V L S   DS S++      
Sbjct: 11  TFSISISTQKLHHHSSKFLNLGFRIRCESGDVSSPLRTKAVSLSSEMEDSSSLKKSLMEL 70

Query: 78  VGTKKKPLP-----------------KPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEA 137
            G K +P P                 KP W+R+  P GE++ ++K+ L  L L+TVCEEA
Sbjct: 71  EGKKSEPYPGGMPKMGPFTGRDPNVKKPAWLRQKAPQGERFQEVKESLSRLNLNTVCEEA 130

Query: 138 KCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIAS 197
           +CPN+GECW+GG  G ATATIM+LGDTCTRGCRFC VKTSR PPPPDP EP N A+AIAS
Sbjct: 131 QCPNIGECWNGGGDGVATATIMVLGDTCTRGCRFCAVKTSRNPPPPDPMEPENTAKAIAS 190

Query: 198 WGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARS 257
           WG+DY+VITSVDRDD+PD GSGHFA+TV+ +K  KP+++IE L  DFRGD   V+ +  S
Sbjct: 191 WGVDYIVITSVDRDDIPDGGSGHFAQTVKAMKRHKPDIMIECLTSDFRGDLEAVDTLVHS 250

Query: 258 GLDVFAHNIETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQ 317
           GLDVFAHN+ETV+ LQ +VRD RA ++QS+ VL  AK  S  G +TKTSIMLG GET ++
Sbjct: 251 GLDVFAHNVETVKRLQRLVRDPRAGYEQSMSVLKHAK-ISKPGMITKTSIMLGLGETDEE 310

Query: 318 VVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPM 377
           + + M  +RA  VD++T GQY++P+  H+ V EY+TPE F+ ++  G  +GFRYVASGP+
Sbjct: 311 LKEAMADLRAIDVDILTLGQYLQPTPLHLTVKEYVTPEKFDFWKTYGESIGFRYVASGPL 370

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B9H5L94.8e-18186.07Lipoyl synthase, mitochondrial OS=Populus trichocarpa OX=3694 GN=LIP1 PE=3 SV=1[more]
Q3LSN54.1e-18082.93Lipoyl synthase 2, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-2 PE=2 SV=1[more]
Q3LSN45.3e-18082.93Lipoyl synthase 1, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-1 PE=2 SV=1[more]
B7FM455.0e-17881.33Lipoyl synthase, mitochondrial OS=Medicago truncatula OX=3880 GN=LIP1 PE=2 SV=1[more]
A5CB816.1e-17684.87Lipoyl synthase, mitochondrial OS=Vitis vinifera OX=29760 GN=LIP1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004136815.11.26e-272100.00lipoyl synthase 2, mitochondrial [Cucumis sativus] >KGN43590.1 hypothetical prot... [more]
XP_016901725.12.85e-26998.69PREDICTED: lipoyl synthase 2, mitochondrial isoform X1 [Cucumis melo] >KAA003159... [more]
XP_038887854.12.62e-26597.11lipoyl synthase 2, mitochondrial [Benincasa hispida][more]
XP_022147922.14.25e-25593.95lipoyl synthase 2, mitochondrial [Momordica charantia][more]
XP_023531015.12.02e-25393.95lipoyl synthase 2, mitochondrial [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0K1R36.12e-273100.00Lipoyl synthase, mitochondrial OS=Cucumis sativus OX=3659 GN=LIP1 PE=3 SV=1[more]
A0A5A7SKJ11.38e-26998.69Lipoyl synthase, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=LIP1 PE... [more]
A0A1S4E1621.38e-26998.69Lipoyl synthase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495529 PE=3 SV=1[more]
A0A6J1D3N52.06e-25593.95Lipoyl synthase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111016734 PE... [more]
A0A6J1ERM28.02e-25393.68Lipoyl synthase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111437162 PE=... [more]
Match NameE-valueIdentityDescription
AT2G20860.11.3e-17681.65lipoic acid synthase 1 [more]
AT5G08415.17.5e-10551.43Radical SAM superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006638Elp3/MiaB/NifBSMARTSM00729MiaBcoord: 129..336
e-value: 5.2E-8
score: 42.6
IPR007197Radical SAMPFAMPF04055Radical_SAMcoord: 136..298
e-value: 2.9E-11
score: 44.0
IPR007197Radical SAMPROSITEPS51918RADICAL_SAMcoord: 124..344
score: 27.702848
IPR003698Lipoyl synthaseTIGRFAMTIGR00510TIGR00510coord: 72..366
e-value: 2.7E-120
score: 399.2
IPR003698Lipoyl synthasePIRSFPIRSF005963Lipoyl_synthcoord: 17..380
e-value: 2.5E-146
score: 485.2
IPR003698Lipoyl synthaseSFLDSFLDF00271lipoyl_synthasecoord: 70..366
e-value: 0.0
score: 435.2
IPR003698Lipoyl synthasePANTHERPTHR10949LIPOYL SYNTHASEcoord: 34..375
IPR003698Lipoyl synthaseHAMAPMF_00206Lipoyl_synthcoord: 78..372
score: 131.906616
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 122..347
e-value: 1.2E-13
score: 52.8
IPR031691Lipoyl synthase, N-terminalPFAMPF16881LIAS_Ncoord: 5..113
e-value: 3.0E-16
score: 59.8
NoneNo IPR availableSFLDSFLDG01058lipoyl_synthase_likecoord: 70..366
e-value: 0.0
score: 435.2
NoneNo IPR availablePANTHERPTHR10949:SF0LIPOYL SYNTHASE, MITOCHONDRIALcoord: 34..375
NoneNo IPR availableCDDcd01335Radical_SAMcoord: 133..313
e-value: 5.94383E-7
score: 47.7134
NoneNo IPR availableSUPERFAMILY102114Radical SAM enzymescoord: 94..369
IPR027527Lipoyl synthase, mitochondrialHAMAPMF_03128Lipoyl_synth_plantMcoord: 20..375
score: 61.664967

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy7G004020.1CsGy7G004020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009107 lipoate biosynthetic process
biological_process GO:0009249 protein lipoylation
cellular_component GO:0005739 mitochondrion
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0016992 lipoate synthase activity
molecular_function GO:0102552 lipoyl synthase activity (acting on glycine-cleavage complex H protein
molecular_function GO:0102553 lipoyl synthase activity (acting on pyruvate dehydrogenase E2 protein)
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0051536 iron-sulfur cluster binding