CsGy7G001070 (gene) Cucumber (Gy14) v2.1

BLAST of CsGy7G001070 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 947.6 bits (2448), Expect = 1.1e-274
Identity = 515/922 (55.86%), Postives = 649/922 (70.39%), Query Frame = 0

Query: 59  DTSDRTEVILKIDG-GGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIG------- 118
           D +DR EVI+KIDG  G+++ VS      G   G +WR+  YDFW + E  +        
Sbjct: 4   DAADRREVIVKIDGENGNNNGVS------GETVGKIWRDGSYDFWTDGEGNLNKGHNAAA 63

Query: 119 -ESARMGDNSG-VDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEEL 178
            +S R    +G   ++EGFEF +     EDPPTKLIG FL KQ+  GE  LDMDL M+EL
Sbjct: 64  VDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGMDEL 123

Query: 179 KPNRIVPPLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPR 238
           + +R + P++ESP   T +D                  + RR+  SN             
Sbjct: 124 Q-SRGLTPVSESPRVSTKRD-----------------PVGRRDSRSN------------- 183

Query: 239 QQPHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSN-LLRAKTKSRLIDPPA 298
                            N    E ++C+ N         QR S+ LL+ +T+SRL DPP 
Sbjct: 184 ---------------TNNNDDGEVVKCSGN-----NAPIQRSSSTLLKMRTRSRLSDPPT 243

Query: 299 ------EPDRLSGLIPKSGQLRSGFLGKI-----EDDDDDPFLEDDLPDEFKRGNFSALT 358
                   D  SG IPKSGQ++SGF GK      E+++DDPF  +DLP+E+++   S   
Sbjct: 244 PQLPPQTADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWI 303

Query: 359 VLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFF 418
           VL+W+SLI+I A  +CTL++P LR+K LWEL +WKWE M+ +LICGRLVS W ++I VFF
Sbjct: 304 VLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFF 363

Query: 419 IERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLV 478
           IERNFLLRKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+++V K  NT  L  V+++ V
Sbjct: 364 IERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFV 423

Query: 479 CLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEE 538
           CLL+  L+WLVKTL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPPL+EI+KNEEEE
Sbjct: 424 CLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEE 483

Query: 539 ERIADEVQKLQNAGIIIPPDLKAATFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRN 598
           ERI+ EV+K QN G +            I+SG +    +T KS        +       N
Sbjct: 484 ERISVEVKKFQNPGGV-----------EIQSGAQKSPMKTGKSPFLSHVLSNGGGGGGEN 543

Query: 599 DGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAK 658
            GITID LHKL+ KNVSAW MKRL+NI+R GS++TLDEQ++ P LDD+   +I+SE EAK
Sbjct: 544 KGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEAK 603

Query: 659 AAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNA 718
            AA+KIF NVA  G K+IY +D++RF+ +DE LKT+SLFEGA+E  RISKS+LKNWVVNA
Sbjct: 604 LAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNA 663

Query: 719 FRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVA 778
           FRERRALALTLNDTKTAV++LH MVN++ GI+IL++WLI+LGI S+KF + +SSQ+VVVA
Sbjct: 664 FRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVA 723

Query: 779 FIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPN 838
           FIFGN CK +FE+II+LFV+HPFDVGDRCEIDG+QMVVEEMNILTTVFLR+DN KV+ PN
Sbjct: 724 FIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPN 783

Query: 839 SVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVF 898
           S+L TK I N+YRSPDMG+ IEF +HI TPAEKI  +K RI SYIEG K+HWYPAPMIVF
Sbjct: 784 SLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVF 843

Query: 899 KDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRS 956
           KD++ LN V++AVW +HRMNHQD GE+WARRS LVEE+ K+C+ELDI+YRL P+DIN+R+
Sbjct: 844 KDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINVRN 853

BLAST of CsGy7G001070 vs. ExPASy Swiss-Prot
Match: F4IME2 (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 PE=2 SV=2)

HSP 1 Score: 892.5 bits (2305), Expect = 4.2e-258
Identity = 502/979 (51.28%), Postives = 668/979 (68.23%), Query Frame = 0

Query: 5   KKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDTSDRT 64
           + SFK + S+K   +I + G  SE + E LPIL +H        + D+ P  DS  S   
Sbjct: 4   RNSFKSHSSYK---QIRSPGDQSEPSPEHLPILHDHHPDHSGMVVDDQKP--DSTRS--- 63

Query: 65  EVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIG---ESARMGDNSGV 124
                            SLD   N    V R++ Y FW ++  G      + R  D   +
Sbjct: 64  -----------------SLDDGRN--APVERDASYKFWQDNTTGTSTDHTAVRTSDKDPI 123

Query: 125 ------DRNEG-FEFVQTGYGMEDPPTKLI-GDFLRKQ---KIEGETTLDMDLEMEELKP 184
                 DR  G F+FV     +++ PTK++ G+ + +Q   +   E TLD+D E +++  
Sbjct: 124 AISRKGDRLSGSFDFVHGKLPVDESPTKMVAGEPVNRQWRGRNNEEITLDVDQENDDVSH 183

Query: 185 NRIVPPLAESPLS-QTSKDLKVSFQ--------------HDSTEISSNDQSIRRRNRDSN 244
             +  P + +  S   S++++VSF                 S+  SS+  +  R N+D  
Sbjct: 184 QTMPTPTSTARTSFDASREMRVSFNVRRAGGAFVAGSVPSSSSHSSSSSSATMRTNQDQP 243

Query: 245 DLKEESKGGQSPRQQPHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLL- 304
            L+EE                              E +RC SN      +SFQRKS L+ 
Sbjct: 244 QLQEE------------------------------EVVRCTSN------MSFQRKSELIS 303

Query: 305 RAKTKSRLIDPPAEPDR-LSGLIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFS 364
           R KT+SRL DPP E +   SG   +SGQL+SG L  I D++DDP  E+D+PDE+KRG   
Sbjct: 304 RVKTRSRLQDPPREEETPYSGW--RSGQLKSGLLADI-DEEDDPLAEEDVPDEYKRGKLD 363

Query: 365 ALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIG 424
           A+T+LQW+SL+ I AAL C+LS+   ++  +W L +WKWEV + +LICGRLVSGWGIRI 
Sbjct: 364 AITLLQWLSLVAIIAALACSLSIQSWKKVRVWNLHLWKWEVFLLVLICGRLVSGWGIRIV 423

Query: 425 VFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSR 484
           VFFIERNFLLRKRVLYFVYGVR+ VQNCLWLGLVL+AWH LF+K+V+++T +  L YV++
Sbjct: 424 VFFIERNFLLRKRVLYFVYGVRRAVQNCLWLGLVLLAWHFLFDKKVQRETRSRFLPYVTK 483

Query: 485 VLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNE 544
           +LVC L+ST++WL+KTL+VKVLASSFHVSTYFDRIQE+LFNQYVIETLSGPP++E+ + E
Sbjct: 484 ILVCFLLSTILWLIKTLVVKVLASSFHVSTYFDRIQEALFNQYVIETLSGPPMIEMSRIE 543

Query: 545 EEEERIADEVQKLQNAGIIIPPDLKAATFASIKSGREIGSGRTHKSFCAKSCKLSRALTK 604
           EEEER  DE+ K+QNAG  +PPDL AA F   KSGR +              KLS  + K
Sbjct: 544 EEEERAQDEIFKMQNAGANLPPDLCAAAFPPGKSGRVMNP------------KLSPIIPK 603

Query: 605 NRND-GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSE 664
           +  D GI+++HLH+++ KN+SAWNMKRL+ IVR  S++TLDEQ+     +DEST +I+SE
Sbjct: 604 STTDNGISMEHLHRMNHKNISAWNMKRLMKIVRNVSLTTLDEQMLESTYEDESTRQIRSE 663

Query: 665 REAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNW 724
           +EAKAAA+KIF+NV  RG KYIYL+DL+RF+REDE +KTM LFEGA EN+RISKSALKNW
Sbjct: 664 KEAKAAARKIFKNVEQRGAKYIYLEDLMRFLREDEAMKTMGLFEGAPENKRISKSALKNW 723

Query: 725 VVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQI 784
           +VNAFRERRALALTLNDTKTAV+KLHHM+N++  I+I+++WL++L IASSK  +F+SSQ+
Sbjct: 724 LVNAFRERRALALTLNDTKTAVNKLHHMINIVTAIVIVVIWLVLLEIASSKVLLFVSSQV 783

Query: 785 VVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKV 844
           V++AFIFGNT KT+FE+IIFLF++HP+DVGDRCEID +Q+VVEEMNILTTVFLRYDNLK+
Sbjct: 784 VLLAFIFGNTVKTVFESIIFLFIVHPYDVGDRCEIDSVQLVVEEMNILTTVFLRYDNLKI 843

Query: 845 IIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAP 904
           + PNS+L  K I+N+YRSPDMG++IEF VHI TP EKI+ +K RI +YI+   E+WYP  
Sbjct: 844 MYPNSLLWQKSINNYYRSPDMGDAIEFCVHITTPLEKISVIKQRISNYIDNKPEYWYPQA 903

Query: 905 MIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDI 952
            I+ KD++ L+ V+LA+W  HR+NHQD  ERW RR+VLVEEV+K+  ELDIQ+R  P+DI
Sbjct: 904 KIIVKDLEDLHIVRLAIWPCHRINHQDMAERWTRRAVLVEEVIKILLELDIQHRFYPLDI 903

BLAST of CsGy7G001070 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 891.0 bits (2301), Expect = 1.2e-257
Identity = 487/924 (52.71%), Postives = 653/924 (70.67%), Query Frame = 0

Query: 59  DTSDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDN 118
           D++DR + I+ I+ G  S AV  +  S+   GGN+W+ES YDFW+ ++   G++ + GD+
Sbjct: 5   DSTDRRDFIVNIN-GQESGAVGATGSSSNAEGGNIWKESSYDFWDGEK---GKNDKKGDD 64

Query: 119 SGVDRNEGFEFVQTG------YGMEDPPTKLIGDFLRKQKIEG-ETTLDMDLEMEELKPN 178
              D    F F Q G        + DPP+KLIG FL KQ+  G E +LD++L M EL+ N
Sbjct: 65  EDED-GGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSN 124

Query: 179 RIVPPLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQP 238
              PP   +  +   + L        T IS +   ++ + +             + R++ 
Sbjct: 125 --TPPRPATASNTPRRGL--------TTISESSSPVKTKVK-----------ADAVRRRQ 184

Query: 239 HHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPA---- 298
           +   LG  +    +N   AE ++C S           +K  L R KTKSRL DPP     
Sbjct: 185 NRTSLGGSSDEEGRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTHP 244

Query: 299 ---EPDRLSGLIPKSGQLRSGFLGKI------------EDDDDDPFLEDDLPDEFKRGNF 358
              + +  SG   +SG  +SGFLGK             E++++DPFL++DLP+EFKR   
Sbjct: 245 AIDKTEMKSG--RRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKL 304

Query: 359 SALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRI 418
           S    L+W+SL++I  +L+C+L++  L+ K+ W+LD+WKWEV + +LICGRLVS W +RI
Sbjct: 305 SFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRI 364

Query: 419 GVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVS 478
            VF +E+NF  RKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+K+VE++T ++ L YV+
Sbjct: 365 IVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVT 424

Query: 479 RVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKN 538
           RVLVCLL++ +IWLVKT++VKVLASSFH+STYFDRIQESLF QYVIETLSGPPL+EI++ 
Sbjct: 425 RVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRM 484

Query: 539 EEEEERIADEVQKLQN-AGIIIPPDLKAATFASIKSGREIGSGRTHKSFCAKSCKLSRAL 598
           EEEE+++A++V+ L+  AG  +PP LK    A++KS  ++G          KS  L+R  
Sbjct: 485 EEEEQQVAEDVKSLEKLAGAKLPPALK----ATVKSFMKVG----------KSPGLNRIG 544

Query: 599 TKNRND--GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEI 658
           +K   D  GI ID L +++TKNVSAWNMKRL+NI+  G+ISTLD+ ++    +DE  T I
Sbjct: 545 SKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHI 604

Query: 659 KSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSAL 718
           +SE EAK AA+KIF NV   G +YIYL+D +RF+ E+E  + M+LFEGA+E+ +ISKS L
Sbjct: 605 RSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCL 664

Query: 719 KNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLS 778
           KNWVV AFRERRALALTLNDTKTAVD+LH ++NV+ GI+I+I+WL++LGIA+++F + LS
Sbjct: 665 KNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLS 724

Query: 779 SQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDN 838
           SQ+++VAF+FGN+CKTIFEAIIFLFVMHPFDVGDRCEIDG+Q+VVEEMNILTTVFLRYDN
Sbjct: 725 SQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDN 784

Query: 839 LKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWY 898
            K+I PNSVL TK I N+YRSPDMG+++EF VHIATP EKITA+K RI+SY++  K++WY
Sbjct: 785 QKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWY 844

Query: 899 PAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLP 951
           PAPMIVF  +D LN VK+AVWL+HRMNHQD GER+ RR +L+EEV K C+ELDI+YRL P
Sbjct: 845 PAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYP 875

BLAST of CsGy7G001070 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 847.4 bits (2188), Expect = 1.5e-244
Identity = 461/922 (50.00%), Postives = 635/922 (68.87%), Query Frame = 0

Query: 60  TSDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNS 119
           T  R + +++IDG                +    WRES  +FW+ND+          D S
Sbjct: 6   TDQRRDFVVRIDG------------EDNGDSEKFWRESSINFWHNDKSSKPPGGEEDDGS 65

Query: 120 GVDRNEGFEFVQTGYGME---DPPTKLIGDFLRKQKIEG-ETTLDMDLEMEELKPNRIVP 179
                  F+F++         DPP+KLI  FL KQK  G E +LDM+  M EL+ N  VP
Sbjct: 66  -------FDFMRRSSEKSEEPDPPSKLINQFLNKQKASGDEISLDMEANMPELQKN-TVP 125

Query: 180 PLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHER 239
           PL+ + +S ++  +       S    + D   RR+NR +  L    K G S   + +   
Sbjct: 126 PLSSTAVSGSASPVTAPVT-ASYRNGTGDAIRRRQNRVT--LSPSVKDGDSSEDEENR-- 185

Query: 240 LGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAE--PDRLS 299
                + G      +E ++C SN       S  R   L++ KT+SRL+DPP    PD +S
Sbjct: 186 -----VDG------SEVVKCTSN------RSTMRTKTLMKMKTRSRLMDPPTPTYPDMVS 245

Query: 300 GLIPKSGQLRSGFLG-------------KIEDDDDDPFLEDDLPDEFKRGNFSALTVLQW 359
           G  P+SG L  GF G             K  ++++DPF E+DLP+  ++       +++W
Sbjct: 246 GRTPRSGNLNPGFSGRNTKPGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEW 305

Query: 360 VSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERN 419
           + LI+I A+LIC+L +PYLR K+LW+L +WKWEVM+ +LICGRLVS W +++ V+F+E N
Sbjct: 306 IFLILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESN 365

Query: 420 FLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLI 479
           FL RK+VLYFVYG+RKPVQNCLWLGLVLIAWH LF+K+VE++  +++L YV++VL+CLL+
Sbjct: 366 FLWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLV 425

Query: 480 STLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIA 539
           + +IWL+KTL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPP +EI     EEE++A
Sbjct: 426 AVIIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEI---HIEEEKVA 485

Query: 540 DEVQKLQNAGIIIPPDLKAATFASIKSGREIGSGRTHK--SFCAKSCKLSRALTKNR--N 599
           ++V+  +  G  + P        S      +GSGR  K  S   KS  LSR+ +K     
Sbjct: 486 NDVKTFEIVGRKLSP--LGPKAVSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGE 545

Query: 600 DGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCL-DDESTTEIKSEREA 659
           +GI IDHL +++TKNVSAW MK+L+N+++ G++STLDEQI+     +D+  T+I+SE EA
Sbjct: 546 EGIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEA 605

Query: 660 KAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVN 719
           K AA+KIFQNVA  G +YIY++D +RF+ EDE  + M LFEGA+E  +ISKS LKNWVVN
Sbjct: 606 KLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVN 665

Query: 720 AFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVV 779
           AFRERRALALTLNDTKTAV++LH +V+V+  I+ILI+WL++LGIA++KF + +SSQ+++V
Sbjct: 666 AFRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLV 725

Query: 780 AFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIP 839
            F+FGN+CKTIFEA+IF+FVMHPFDVGDRCEIDG+QM+VEEMNILTTVFLR+DN K++ P
Sbjct: 726 VFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYP 785

Query: 840 NSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIV 899
           NS+L TK I N+YRSPDM ++IEF VHIATP EK TA++ RI+SY++  K+HW+P+PMIV
Sbjct: 786 NSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIV 845

Query: 900 FKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIR 956
           F+D+ GLN VK+A+W +H+MNHQ+ GER+ RR  L+EE+ ++C+ELDI+YRL P++IN++
Sbjct: 846 FRDMCGLNSVKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVK 880

BLAST of CsGy7G001070 vs. ExPASy Swiss-Prot
Match: F4IME1 (Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana OX=3702 GN=MSL7 PE=2 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 6.1e-225
Identity = 399/689 (57.91%), Postives = 532/689 (77.21%), Query Frame = 0

Query: 269 RKSNLL-RAKTKSRLIDPPAEPD-RLSGLIPKSGQLRSGFLGKIEDD---DDDPFLEDDL 328
           RK+ L+ RAK +SRLIDPP E + + S  I  S QLRSG LG+  DD   +DD   E+D+
Sbjct: 161 RKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSAEEDV 220

Query: 329 PDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGR 388
           P E+++    A+T+LQW+SLI +  AL+ +L +   R  +LW L +WKWEV++ +LICGR
Sbjct: 221 PVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGR 280

Query: 389 LVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQT 448
           LVSG GIRI VFFIERNFLLRKRVLYFVYGV+  VQNCLWLGLVL+AWH LF+K+VEK+T
Sbjct: 281 LVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKET 340

Query: 449 NTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSG 508
            + +L  +S++LVC L+ST++WL+KTL+VKVLASSFHVSTYFDRIQE+LF+ Y+IETLSG
Sbjct: 341 QSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLSG 400

Query: 509 PPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASIKSGREIGSGRTHKSFCAK 568
           PP++E+ + EEEE+R  DE+ K+Q  G  + P+L +A F   KSG  +            
Sbjct: 401 PPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTM------------ 460

Query: 569 SCKLSRALTKNRND-GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLD 628
           + K S  + K  +D GIT+D LHK++ KNVSAWNMKRL+ IVR  S+STLDEQ      +
Sbjct: 461 NMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCE 520

Query: 629 DESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQ 688
           DEST +I+SE+EAKAAA+KIF+NVA  G K+IYL+DL+RF+R DE +KTM LFEGA   +
Sbjct: 521 DESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTK 580

Query: 689 RISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASS 748
           +I+KSALKNW+VNAFRERRALALTLNDTKTAV+KLHHM++ +  I+I+++WLI+L IA+S
Sbjct: 581 KITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATS 640

Query: 749 KFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTT 808
           K+ +FL+SQ+V++AF+FGN+ KT+FE+IIFLF++HP+DVGDR  ID ++MVVEEMNILTT
Sbjct: 641 KYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTT 700

Query: 809 VFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIE 868
           VFLR DNLK++ PN +L  K IHN+ RSPDMG+ +   VHI TP EKI A+K RI SYI+
Sbjct: 701 VFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYID 760

Query: 869 GNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELD 928
              E+WYP   ++ KD++ LN V++A+WL H++NHQ+ GER+ RR++L+EEV+K+  ELD
Sbjct: 761 SKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELD 820

Query: 929 IQYRLLPIDINIRSLPSSAPSIGFPSNWT 952
           IQYR  P+DIN++++P+   S   P  W+
Sbjct: 821 IQYRFHPLDINVKTMPTVVSS-RVPPAWS 836

BLAST of CsGy7G001070 vs. NCBI nr
Match: XP_004144925.1 (mechanosensitive ion channel protein 6 isoform X1 [Cucumis sativus] >KGN43281.1 hypothetical protein Csa_020565 [Cucumis sativus])

HSP 1 Score: 1858 bits (4812), Expect = 0.0
Identity = 954/955 (99.90%), Postives = 954/955 (99.90%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60
           MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60

Query: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG 120
           SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG
Sbjct: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG 120

Query: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRIVPPLAES 180
           VDRNEGFEFVQTGYGMEDPPTKLIGDFL KQKIEGETTLDMDLEMEELKPNRIVPPLAES
Sbjct: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLCKQKIEGETTLDMDLEMEELKPNRIVPPLAES 180

Query: 181 PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPT 240
           PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPT
Sbjct: 181 PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPT 240

Query: 241 ISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSG 300
           ISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSG
Sbjct: 241 ISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSG 300

Query: 301 QLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR 360
           QLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR
Sbjct: 301 QLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR 360

Query: 361 EKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN 420
           EKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN
Sbjct: 361 EKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN 420

Query: 421 CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH 480
           CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH
Sbjct: 421 CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH 480

Query: 481 VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAA 540
           VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAA
Sbjct: 481 VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAA 540

Query: 541 TFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRL 600
           TFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRL
Sbjct: 541 TFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRL 600

Query: 601 LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLV 660
           LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLV
Sbjct: 601 LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLV 660

Query: 661 RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM 720
           RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM
Sbjct: 661 RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM 720

Query: 721 VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD 780
           VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD
Sbjct: 721 VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD 780

Query: 781 VGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFL 840
           VGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFL
Sbjct: 781 VGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFL 840

Query: 841 VHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDS 900
           VHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDS
Sbjct: 841 VHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDS 900

Query: 901 GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 955
           GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS
Sbjct: 901 GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 955

BLAST of CsGy7G001070 vs. NCBI nr
Match: XP_008448113.1 (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis melo])

HSP 1 Score: 1697 bits (4394), Expect = 0.0
Identity = 888/961 (92.40%), Postives = 902/961 (93.86%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60
           MDTLKKSFKGNVSFKHTRKISAGG  SEINHEELPILLNHQ+TDH       H VNDSD 
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDH-------HLVNDSDP 60

Query: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESA------R 120
           SDRTEVILKID GGSS AVSRSLDS  NNGG VWRESRYDFWNNDE GIGESA      R
Sbjct: 61  SDRTEVILKIDDGGSS-AVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGAR 120

Query: 121 MGDNSGVDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRIV 180
           M D SG D NEGF+FVQTGYGMEDPPTKLIGDFL KQKI GETTLDMDLEMEELKPNRI+
Sbjct: 121 MSD-SGDDGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRII 180

Query: 181 PPLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHE 240
           PPLAESPLSQTSKDLKVSFQ DSTEISSNDQS+RRR RDS+DLKEE KG Q P QQ HHE
Sbjct: 181 PPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHE 240

Query: 241 RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSG 300
           RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRL DPPAEPDRLSG
Sbjct: 241 RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSG 300

Query: 301 LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTL 360
           LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPD+FKRGNF+ALTVLQW SLI+ITAA ICTL
Sbjct: 301 LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTL 360

Query: 361 SVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGV 420
           SVPYLREKSLWELDIWKWEVMI ILICGRLVSGWGIRI VFFIERNFLLRKRVLYFVYGV
Sbjct: 361 SVPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGV 420

Query: 421 RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV 480
           RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV
Sbjct: 421 RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV 480

Query: 481 LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIP 540
           LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAG+ IP
Sbjct: 481 LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIP 540

Query: 541 PDLKAATFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSA 600
           PDLKA TFASIKSGR I S RTHKSFCAKS K SRALTKN NDGITIDHLHKLS KNVSA
Sbjct: 541 PDLKA-TFASIKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSA 600

Query: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYI 660
           WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVA RGYKYI
Sbjct: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYI 660

Query: 661 YLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAV 720
           YLDDL+RFMREDEVLKT SLFEGAAEN+RISKS LKNWVVN FRERRALALTLNDTKTAV
Sbjct: 661 YLDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAV 720

Query: 721 DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF 780
           DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF
Sbjct: 721 DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF 780

Query: 781 VMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMG 840
           VMHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYRSPDMG
Sbjct: 781 VMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMG 840

Query: 841 ESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHR 900
           ES+EF VHIATPAEKITAMK RIISYIEGNKEHW PAPMIVFKDIDGLNK+KLAVWLSHR
Sbjct: 841 ESVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHR 900

Query: 901 MNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA 955
           MNHQDS ERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA
Sbjct: 901 MNHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA 951

BLAST of CsGy7G001070 vs. NCBI nr
Match: KAA0040324.1 (mechanosensitive ion channel protein 6-like [Cucumis melo var. makuwa] >TYK23381.1 mechanosensitive ion channel protein 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 1694 bits (4388), Expect = 0.0
Identity = 887/961 (92.30%), Postives = 901/961 (93.76%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60
           MDTLKKSFKGNVSFKHTRKISAGG  SEINHEELPILLNHQ+TDH       H VNDSD 
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDH-------HLVNDSDP 60

Query: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESA------R 120
           SDRTEVILKID  GSS AVSRSLDS  NNGG VWRESRYDFWNNDE GIGESA      R
Sbjct: 61  SDRTEVILKIDDSGSS-AVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGAR 120

Query: 121 MGDNSGVDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRIV 180
           M D SG D NEGF+FVQTGYGMEDPPTKLIGDFL KQKI GETTLDMDLEMEELKPNRI+
Sbjct: 121 MSD-SGDDGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRII 180

Query: 181 PPLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHE 240
           PPLAESPLSQTSKDLKVSFQ DSTEISSNDQS+RRR RDS+DLKEE KG Q P QQ HHE
Sbjct: 181 PPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHE 240

Query: 241 RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSG 300
           RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRL DPPAEPDRLSG
Sbjct: 241 RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSG 300

Query: 301 LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTL 360
           LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPD+FKRGNF+ALTVLQW SLI+ITAA ICTL
Sbjct: 301 LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTL 360

Query: 361 SVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGV 420
           SVPYLREKSLWELDIWKWEVMI ILICGRLVSGWGIRI VFFIERNFLLRKRVLYFVYGV
Sbjct: 361 SVPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGV 420

Query: 421 RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV 480
           RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV
Sbjct: 421 RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV 480

Query: 481 LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIP 540
           LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAG+ IP
Sbjct: 481 LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIP 540

Query: 541 PDLKAATFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSA 600
           PDLKA TFASIKSGR I S RTHKSFCAKS K SRALTKN NDGITIDHLHKLS KNVSA
Sbjct: 541 PDLKA-TFASIKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSA 600

Query: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYI 660
           WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVA RGYKYI
Sbjct: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYI 660

Query: 661 YLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAV 720
           YLDDL+RFMREDEVLKT SLFEGAAEN+RISKS LKNWVVN FRERRALALTLNDTKTAV
Sbjct: 661 YLDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAV 720

Query: 721 DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF 780
           DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF
Sbjct: 721 DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF 780

Query: 781 VMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMG 840
           VMHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYRSPDMG
Sbjct: 781 VMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMG 840

Query: 841 ESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHR 900
           ES+EF VHIATPAEKITAMK RIISYIEGNKEHW PAPMIVFKDIDGLNK+KLAVWLSHR
Sbjct: 841 ESVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHR 900

Query: 901 MNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA 955
           MNHQDS ERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA
Sbjct: 901 MNHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA 951

BLAST of CsGy7G001070 vs. NCBI nr
Match: XP_038887578.1 (mechanosensitive ion channel protein 6-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1669 bits (4322), Expect = 0.0
Identity = 865/961 (90.01%), Postives = 901/961 (93.76%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60
           MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNH+  DH R LRDRHPVNDSD+
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHEPADHRRRLRDRHPVNDSDS 60

Query: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESA------R 120
           SD TEVILKID GGSS+ VSRSLDS G+NGG VWRESRY FWNN+ IGIGESA      R
Sbjct: 61  SDGTEVILKIDDGGSST-VSRSLDSVGSNGGKVWRESRYGFWNNNAIGIGESASRVSGAR 120

Query: 121 MGDNSGVDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRIV 180
           M D SGVDRNEGFEFVQ GYGMEDPPTKLIG+FL KQKI GETTLDMDLEMEELKP+R +
Sbjct: 121 MSD-SGVDRNEGFEFVQPGYGMEDPPTKLIGEFLHKQKIRGETTLDMDLEMEELKPDRSM 180

Query: 181 PPLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHE 240
            PL ESPL+QTSKDLKVSFQ DSTEISSNDQS+RRR RDS DL EE KGGQ P QQ HHE
Sbjct: 181 TPLPESPLNQTSKDLKVSFQQDSTEISSNDQSVRRRCRDSRDLPEEFKGGQPPWQQSHHE 240

Query: 241 RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSG 300
           RLGSPTISGVQNES AEAMR ASNLSFHSELSFQRKSNLLRAKTKSRLID PAEPDRLSG
Sbjct: 241 RLGSPTISGVQNESSAEAMRSASNLSFHSELSFQRKSNLLRAKTKSRLIDSPAEPDRLSG 300

Query: 301 LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTL 360
           LIPKSGQLRSGFLGKI+DDDDDPFLE+DLPD+FKRGNF+ALTVLQW+SLI+ITAAL+CTL
Sbjct: 301 LIPKSGQLRSGFLGKIDDDDDDPFLEEDLPDDFKRGNFNALTVLQWISLILITAALVCTL 360

Query: 361 SVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGV 420
           S+PYLREKSLWELDIWKWEVMI ILICGRLVSGWGI+I VFFIERNFLLRK+VLYFVYGV
Sbjct: 361 SIPYLREKSLWELDIWKWEVMILILICGRLVSGWGIKIVVFFIERNFLLRKKVLYFVYGV 420

Query: 421 RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV 480
           RKPVQNCLWLGLVLIAWHLLFNKRVEKQTN SILNYV RVLVCLLIST +WLVKTLMVKV
Sbjct: 421 RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNASILNYVRRVLVCLLISTFLWLVKTLMVKV 480

Query: 481 LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIP 540
           LASSFHVSTYFDRIQESLFNQYVIETLSGPPL+EI KNEEEEER+ADEVQKLQNAGI IP
Sbjct: 481 LASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEILKNEEEEERVADEVQKLQNAGINIP 540

Query: 541 PDLKAATFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSA 600
            DLKAATF+S+KSGR IGSGRTHKSFCAKS KLSRALTKN NDGITIDHLHKLS KNVSA
Sbjct: 541 SDLKAATFSSVKSGRVIGSGRTHKSFCAKSSKLSRALTKNGNDGITIDHLHKLSPKNVSA 600

Query: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYI 660
           WNMKRLLNIVRYGSISTLDEQI+GP  DDESTTEIKSEREAKAAAKKIFQNVA RGYKYI
Sbjct: 601 WNMKRLLNIVRYGSISTLDEQIQGPGRDDESTTEIKSEREAKAAAKKIFQNVARRGYKYI 660

Query: 661 YLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAV 720
           YLDDL+RFMREDEV KTMSLFEGAAEN+RISKSALKNWVVNAFRERRALALTLNDTKTAV
Sbjct: 661 YLDDLMRFMREDEVFKTMSLFEGAAENRRISKSALKNWVVNAFRERRALALTLNDTKTAV 720

Query: 721 DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF 780
           DKLHHMVNVIF ILILILWLIVLGIASSKFF+FLSSQIVVVAFIFGNTCKTIFEAIIFLF
Sbjct: 721 DKLHHMVNVIFSILILILWLIVLGIASSKFFVFLSSQIVVVAFIFGNTCKTIFEAIIFLF 780

Query: 781 VMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMG 840
           VMHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYRSP+MG
Sbjct: 781 VMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPEMG 840

Query: 841 ESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHR 900
           ES+EF VHIATPAEKITAMK RIISYIEGNKEHW P+PM+VF DID LNK+KLAVWLSHR
Sbjct: 841 ESVEFYVHIATPAEKITAMKQRIISYIEGNKEHWCPSPMVVFMDIDDLNKLKLAVWLSHR 900

Query: 901 MNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA 955
           MNHQD+GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTS A
Sbjct: 901 MNHQDAGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSSA 959

BLAST of CsGy7G001070 vs. NCBI nr
Match: XP_031744328.1 (mechanosensitive ion channel protein 6 isoform X2 [Cucumis sativus])

HSP 1 Score: 1532 bits (3967), Expect = 0.0
Identity = 790/791 (99.87%), Postives = 790/791 (99.87%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60
           MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60

Query: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG 120
           SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG
Sbjct: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG 120

Query: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRIVPPLAES 180
           VDRNEGFEFVQTGYGMEDPPTKLIGDFL KQKIEGETTLDMDLEMEELKPNRIVPPLAES
Sbjct: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLCKQKIEGETTLDMDLEMEELKPNRIVPPLAES 180

Query: 181 PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPT 240
           PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPT
Sbjct: 181 PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPT 240

Query: 241 ISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSG 300
           ISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSG
Sbjct: 241 ISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSG 300

Query: 301 QLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR 360
           QLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR
Sbjct: 301 QLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR 360

Query: 361 EKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN 420
           EKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN
Sbjct: 361 EKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN 420

Query: 421 CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH 480
           CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH
Sbjct: 421 CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH 480

Query: 481 VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAA 540
           VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAA
Sbjct: 481 VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAA 540

Query: 541 TFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRL 600
           TFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRL
Sbjct: 541 TFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRL 600

Query: 601 LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLV 660
           LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLV
Sbjct: 601 LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLV 660

Query: 661 RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM 720
           RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM
Sbjct: 661 RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM 720

Query: 721 VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD 780
           VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD
Sbjct: 721 VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD 780

Query: 781 VGDRCEIDGMQ 791
           VGDRCEIDGMQ
Sbjct: 781 VGDRCEIDGMQ 791

BLAST of CsGy7G001070 vs. ExPASy TrEMBL
Match: A0A0A0K619 (Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_7G017150 PE=3 SV=1)

HSP 1 Score: 1858 bits (4812), Expect = 0.0
Identity = 954/955 (99.90%), Postives = 954/955 (99.90%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60
           MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60

Query: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG 120
           SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG
Sbjct: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG 120

Query: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRIVPPLAES 180
           VDRNEGFEFVQTGYGMEDPPTKLIGDFL KQKIEGETTLDMDLEMEELKPNRIVPPLAES
Sbjct: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLCKQKIEGETTLDMDLEMEELKPNRIVPPLAES 180

Query: 181 PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPT 240
           PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPT
Sbjct: 181 PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPT 240

Query: 241 ISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSG 300
           ISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSG
Sbjct: 241 ISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSG 300

Query: 301 QLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR 360
           QLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR
Sbjct: 301 QLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR 360

Query: 361 EKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN 420
           EKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN
Sbjct: 361 EKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN 420

Query: 421 CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH 480
           CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH
Sbjct: 421 CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH 480

Query: 481 VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAA 540
           VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAA
Sbjct: 481 VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAA 540

Query: 541 TFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRL 600
           TFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRL
Sbjct: 541 TFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRL 600

Query: 601 LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLV 660
           LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLV
Sbjct: 601 LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLV 660

Query: 661 RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM 720
           RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM
Sbjct: 661 RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM 720

Query: 721 VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD 780
           VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD
Sbjct: 721 VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD 780

Query: 781 VGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFL 840
           VGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFL
Sbjct: 781 VGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFL 840

Query: 841 VHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDS 900
           VHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDS
Sbjct: 841 VHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDS 900

Query: 901 GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 955
           GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS
Sbjct: 901 GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 955

BLAST of CsGy7G001070 vs. ExPASy TrEMBL
Match: A0A1S3BID3 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103490400 PE=3 SV=1)

HSP 1 Score: 1697 bits (4394), Expect = 0.0
Identity = 888/961 (92.40%), Postives = 902/961 (93.86%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60
           MDTLKKSFKGNVSFKHTRKISAGG  SEINHEELPILLNHQ+TDH       H VNDSD 
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDH-------HLVNDSDP 60

Query: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESA------R 120
           SDRTEVILKID GGSS AVSRSLDS  NNGG VWRESRYDFWNNDE GIGESA      R
Sbjct: 61  SDRTEVILKIDDGGSS-AVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGAR 120

Query: 121 MGDNSGVDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRIV 180
           M D SG D NEGF+FVQTGYGMEDPPTKLIGDFL KQKI GETTLDMDLEMEELKPNRI+
Sbjct: 121 MSD-SGDDGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRII 180

Query: 181 PPLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHE 240
           PPLAESPLSQTSKDLKVSFQ DSTEISSNDQS+RRR RDS+DLKEE KG Q P QQ HHE
Sbjct: 181 PPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHE 240

Query: 241 RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSG 300
           RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRL DPPAEPDRLSG
Sbjct: 241 RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSG 300

Query: 301 LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTL 360
           LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPD+FKRGNF+ALTVLQW SLI+ITAA ICTL
Sbjct: 301 LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTL 360

Query: 361 SVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGV 420
           SVPYLREKSLWELDIWKWEVMI ILICGRLVSGWGIRI VFFIERNFLLRKRVLYFVYGV
Sbjct: 361 SVPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGV 420

Query: 421 RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV 480
           RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV
Sbjct: 421 RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV 480

Query: 481 LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIP 540
           LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAG+ IP
Sbjct: 481 LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIP 540

Query: 541 PDLKAATFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSA 600
           PDLKA TFASIKSGR I S RTHKSFCAKS K SRALTKN NDGITIDHLHKLS KNVSA
Sbjct: 541 PDLKA-TFASIKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSA 600

Query: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYI 660
           WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVA RGYKYI
Sbjct: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYI 660

Query: 661 YLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAV 720
           YLDDL+RFMREDEVLKT SLFEGAAEN+RISKS LKNWVVN FRERRALALTLNDTKTAV
Sbjct: 661 YLDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAV 720

Query: 721 DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF 780
           DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF
Sbjct: 721 DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF 780

Query: 781 VMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMG 840
           VMHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYRSPDMG
Sbjct: 781 VMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMG 840

Query: 841 ESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHR 900
           ES+EF VHIATPAEKITAMK RIISYIEGNKEHW PAPMIVFKDIDGLNK+KLAVWLSHR
Sbjct: 841 ESVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHR 900

Query: 901 MNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA 955
           MNHQDS ERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA
Sbjct: 901 MNHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA 951

BLAST of CsGy7G001070 vs. ExPASy TrEMBL
Match: A0A5D3DI81 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004470 PE=3 SV=1)

HSP 1 Score: 1694 bits (4388), Expect = 0.0
Identity = 887/961 (92.30%), Postives = 901/961 (93.76%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60
           MDTLKKSFKGNVSFKHTRKISAGG  SEINHEELPILLNHQ+TDH       H VNDSD 
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDH-------HLVNDSDP 60

Query: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESA------R 120
           SDRTEVILKID  GSS AVSRSLDS  NNGG VWRESRYDFWNNDE GIGESA      R
Sbjct: 61  SDRTEVILKIDDSGSS-AVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGAR 120

Query: 121 MGDNSGVDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRIV 180
           M D SG D NEGF+FVQTGYGMEDPPTKLIGDFL KQKI GETTLDMDLEMEELKPNRI+
Sbjct: 121 MSD-SGDDGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRII 180

Query: 181 PPLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHE 240
           PPLAESPLSQTSKDLKVSFQ DSTEISSNDQS+RRR RDS+DLKEE KG Q P QQ HHE
Sbjct: 181 PPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHE 240

Query: 241 RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSG 300
           RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRL DPPAEPDRLSG
Sbjct: 241 RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSG 300

Query: 301 LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTL 360
           LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPD+FKRGNF+ALTVLQW SLI+ITAA ICTL
Sbjct: 301 LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTL 360

Query: 361 SVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGV 420
           SVPYLREKSLWELDIWKWEVMI ILICGRLVSGWGIRI VFFIERNFLLRKRVLYFVYGV
Sbjct: 361 SVPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGV 420

Query: 421 RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV 480
           RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV
Sbjct: 421 RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV 480

Query: 481 LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIP 540
           LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAG+ IP
Sbjct: 481 LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIP 540

Query: 541 PDLKAATFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSA 600
           PDLKA TFASIKSGR I S RTHKSFCAKS K SRALTKN NDGITIDHLHKLS KNVSA
Sbjct: 541 PDLKA-TFASIKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSA 600

Query: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYI 660
           WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVA RGYKYI
Sbjct: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYI 660

Query: 661 YLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAV 720
           YLDDL+RFMREDEVLKT SLFEGAAEN+RISKS LKNWVVN FRERRALALTLNDTKTAV
Sbjct: 661 YLDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAV 720

Query: 721 DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF 780
           DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF
Sbjct: 721 DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF 780

Query: 781 VMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMG 840
           VMHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYRSPDMG
Sbjct: 781 VMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMG 840

Query: 841 ESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHR 900
           ES+EF VHIATPAEKITAMK RIISYIEGNKEHW PAPMIVFKDIDGLNK+KLAVWLSHR
Sbjct: 841 ESVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHR 900

Query: 901 MNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA 955
           MNHQDS ERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA
Sbjct: 901 MNHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA 951

BLAST of CsGy7G001070 vs. ExPASy TrEMBL
Match: A0A6J1C288 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111007608 PE=3 SV=1)

HSP 1 Score: 1447 bits (3745), Expect = 0.0
Identity = 763/961 (79.40%), Postives = 837/961 (87.10%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60
           MDT+KKSFK NVSFKHTRKISAGG G+EI+H+ELPILL+H+     R  R R  +ND D 
Sbjct: 1   MDTIKKSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHRH-RGRRSMNDCDP 60

Query: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG 120
           SDRTEVILKID GGS+ AVSR ++    NGG VWRE+ YDFWNND  G   ++R    S 
Sbjct: 61  SDRTEVILKIDDGGSA-AVSRPVEG---NGGKVWRETNYDFWNNDGKGENGASRASGASR 120

Query: 121 V---------DRNEGFEFVQTGYGME--DPPTKLIGDFLRKQKIEGETTLDMDLEMEELK 180
           V         DRNEGFEFVQ GYG+E  DPP KLIG+FL KQK+ GETTLDMDLEMEEL+
Sbjct: 121 VSGARASDNGDRNEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEELQ 180

Query: 181 PNRIVPPLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQ 240
            +RI+PPLAESPLSQTSKDLKVSFQ DSTE SSND SIRRR RDS +L++E KG QSP Q
Sbjct: 181 QDRIIPPLAESPLSQTSKDLKVSFQQDSTESSSND-SIRRRYRDSRELQDEYKGQQSPWQ 240

Query: 241 QPHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEP 300
           Q HHER GSPTISGVQN+  AEA RC SNLSF  ELSFQR S+LLRAKTKSRL+DPP EP
Sbjct: 241 QSHHERHGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDPPEEP 300

Query: 301 DRLSGLIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAA 360
             LSGLIPKSG LRSGFLGK E++DDDPFLE+DLP++FKR +FS LT+LQW+SL++IT A
Sbjct: 301 ALLSGLIPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGA 360

Query: 361 LICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLY 420
           L+CTL +PYLR  SLWEL+IWKWEVM+ ILICGRLVSGWGIRI VF IERNFLLRKR+LY
Sbjct: 361 LVCTLYIPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLY 420

Query: 421 FVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKT 480
           FVYGVRKPVQNC+WLGLVLIAW LLFNKRVE +TN ++L YV RVLV LL+STLIWLVKT
Sbjct: 421 FVYGVRKPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKT 480

Query: 481 LMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNA 540
           LMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPL+EIRKNEE EER+ADEVQKLQNA
Sbjct: 481 LMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNA 540

Query: 541 GIIIPPDLKAATFASIKSGREIGSGRTHKSFCAKSC--KLSRALTKNRNDGITIDHLHKL 600
           G+ IPPDLKA T +S+KSGR IG G   KS   KS   K SR LTKN NDGITIDHLHKL
Sbjct: 541 GVSIPPDLKADT-SSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGNDGITIDHLHKL 600

Query: 601 STKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVA 660
           S KNVSAWNMKRLLN+VRYGSISTLDEQI GPCL+DEST EI+SEREAKAAAKKIFQNVA
Sbjct: 601 SPKNVSAWNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIFQNVA 660

Query: 661 HRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTL 720
            R  KYIY +DL+RFMREDEVL+TMSLFEGA E++RISKS+LKNWVVNAFRERR+LALTL
Sbjct: 661 RRELKYIYPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLALTL 720

Query: 721 NDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIF 780
           NDTKTAVD+LHHMVNVIFGILILILWLI+LGIASSKFF F+SSQIVVVAFIFGNTCKTIF
Sbjct: 721 NDTKTAVDRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTIF 780

Query: 781 EAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNF 840
           EAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNF
Sbjct: 781 EAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNF 840

Query: 841 YRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKL 900
           YRSPDMGES+EF +HIATPAE I  MK RIISYIEGNK HW P+PM VFKD++ LN+++L
Sbjct: 841 YRSPDMGESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRLRL 900

Query: 901 AVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFP 948
           AVWLSHRMNHQDSGERWARRSVLVE+VVKVCQELDIQYRLLPIDIN+ SLPSSAPS+GF 
Sbjct: 901 AVWLSHRMNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAPSMGFT 954

BLAST of CsGy7G001070 vs. ExPASy TrEMBL
Match: A0A6J1EJZ4 (mechanosensitive ion channel protein 6-like OS=Cucurbita moschata OX=3662 GN=LOC111435046 PE=4 SV=1)

HSP 1 Score: 1292 bits (3343), Expect = 0.0
Identity = 674/936 (72.01%), Postives = 788/936 (84.19%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60
           MD+ KKS K N  FKH+RKISAGGAGSEIN EELPILL+HQ +D     + R P ND + 
Sbjct: 1   MDSFKKSLKHNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRPKVRRPANDGEP 60

Query: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFW-NNDEIGIGESARMGDNS 120
           S  +EVILK+D GGSS AVSRS +SAG  GG VWRESRYDFW NND IG G SA     +
Sbjct: 61  SGHSEVILKVDDGGSS-AVSRSQESAGY-GGKVWRESRYDFWSNNDGIGTGGSASWASGA 120

Query: 121 GV----DRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEELKPNRIVP 180
                 DRNEGFEFV+ G G +DPPTKLIGDFL KQK+ GETTLD+DLEMEEL+ +R   
Sbjct: 121 RASDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKT 180

Query: 181 PLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHER 240
           P+ +SPLS+ SKDLKVSFQHDSTEISSN+ S+RRR ++  +++EESK GQ    Q HHE 
Sbjct: 181 PMMDSPLSRASKDLKVSFQHDSTEISSNE-SVRRRYKEFREMQEESKRGQPSWHQSHHEH 240

Query: 241 LGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGL 300
            GS +ISGVQN+S+AEAMR ASNLSF S LS  RKS+LL+AK KSRL DPPAEPDRLSGL
Sbjct: 241 GGS-SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGL 300

Query: 301 IPKSGQLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLS 360
           + KSGQLRSGFLG+ +D++DDPFLE+D PD+F+RG   ALTVLQW+SL +ITAAL+CTLS
Sbjct: 301 VNKSGQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLS 360

Query: 361 VPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVR 420
           + +LRE SLWE  IWKWEVM+ +LICGRLVSGWGI IGVFFIERNFLLRK+VLYFVYGV+
Sbjct: 361 IRHLRENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGVK 420

Query: 421 KPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVL 480
           +PVQNCLWLGLVLIAWHLLF+KRV+ QTN+ IL+YV + LVCLL+ TLIWL KTLM+KVL
Sbjct: 421 RPVQNCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLGTLIWLAKTLMLKVL 480

Query: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPP 540
           ASSFHVSTYFDRIQESLFNQYVIETLSGPP +E++K+EEE+++IADEVQ+LQ+AG+ +PP
Sbjct: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPP 540

Query: 541 DLKAATFASIKSGREIGSG-RTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSA 600
           DL+AA  +SIKSG  + S   + +S   K  KLSR  TKN ++GI IDHLHKL++KNVSA
Sbjct: 541 DLRAAALSSIKSGERVRSNVGSQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSA 600

Query: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTT-EIKSEREAKAAAKKIFQNVAHRGYKY 660
           WNMKRLL IVRYGSI+TLDEQI+GP LDDESTT EIKSEREAKAAAKKIFQNVA  G+KY
Sbjct: 601 WNMKRLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGFKY 660

Query: 661 IYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTA 720
           IYLDDL+RFM+E+EVLKT+S FEGA E +RISKSALKNWVVNAFRERRAL+LTLNDTKTA
Sbjct: 661 IYLDDLMRFMKEEEVLKTLSRFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTA 720

Query: 721 VDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFL 780
           V++L  +VNVIF I IL+LW+I+LGIAS K  +++SSQIV+VAFIFGNT K IFEAIIFL
Sbjct: 721 VERLGLVVNVIFSIFILVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFL 780

Query: 781 FVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDM 840
           FVMHPFDVGDRCEID +QMVVEEMNILTTVFLR+DNLK+I+PNSVLATK+IHN YRSPDM
Sbjct: 781 FVMHPFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDM 840

Query: 841 GESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSH 900
           GE IE  VHI TP EKITAMK RIIS+IE +KEHW P+P+I+ KDID  +K+ +++WLSH
Sbjct: 841 GEFIEICVHIMTPLEKITAMKQRIISFIEADKEHWCPSPIILMKDIDSADKLAMSIWLSH 900

Query: 901 RMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLP 929
            MNHQD  ERWARRSV+VEEV+K+CQE DI   L+P
Sbjct: 901 TMNHQDIRERWARRSVVVEEVLKICQEFDITPTLVP 932

BLAST of CsGy7G001070 vs. TAIR 10
Match: AT1G78610.1 (mechanosensitive channel of small conductance-like 6 )

HSP 1 Score: 947.6 bits (2448), Expect = 7.8e-276
Identity = 515/922 (55.86%), Postives = 649/922 (70.39%), Query Frame = 0

Query: 59  DTSDRTEVILKIDG-GGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIG------- 118
           D +DR EVI+KIDG  G+++ VS      G   G +WR+  YDFW + E  +        
Sbjct: 4   DAADRREVIVKIDGENGNNNGVS------GETVGKIWRDGSYDFWTDGEGNLNKGHNAAA 63

Query: 119 -ESARMGDNSG-VDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGETTLDMDLEMEEL 178
            +S R    +G   ++EGFEF +     EDPPTKLIG FL KQ+  GE  LDMDL M+EL
Sbjct: 64  VDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGMDEL 123

Query: 179 KPNRIVPPLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPR 238
           + +R + P++ESP   T +D                  + RR+  SN             
Sbjct: 124 Q-SRGLTPVSESPRVSTKRD-----------------PVGRRDSRSN------------- 183

Query: 239 QQPHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSN-LLRAKTKSRLIDPPA 298
                            N    E ++C+ N         QR S+ LL+ +T+SRL DPP 
Sbjct: 184 ---------------TNNNDDGEVVKCSGN-----NAPIQRSSSTLLKMRTRSRLSDPPT 243

Query: 299 ------EPDRLSGLIPKSGQLRSGFLGKI-----EDDDDDPFLEDDLPDEFKRGNFSALT 358
                   D  SG IPKSGQ++SGF GK      E+++DDPF  +DLP+E+++   S   
Sbjct: 244 PQLPPQTADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWI 303

Query: 359 VLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFF 418
           VL+W+SLI+I A  +CTL++P LR+K LWEL +WKWE M+ +LICGRLVS W ++I VFF
Sbjct: 304 VLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFF 363

Query: 419 IERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLV 478
           IERNFLLRKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+++V K  NT  L  V+++ V
Sbjct: 364 IERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFV 423

Query: 479 CLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEE 538
           CLL+  L+WLVKTL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPPL+EI+KNEEEE
Sbjct: 424 CLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEE 483

Query: 539 ERIADEVQKLQNAGIIIPPDLKAATFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRN 598
           ERI+ EV+K QN G +            I+SG +    +T KS        +       N
Sbjct: 484 ERISVEVKKFQNPGGV-----------EIQSGAQKSPMKTGKSPFLSHVLSNGGGGGGEN 543

Query: 599 DGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAK 658
            GITID LHKL+ KNVSAW MKRL+NI+R GS++TLDEQ++ P LDD+   +I+SE EAK
Sbjct: 544 KGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEAK 603

Query: 659 AAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNA 718
            AA+KIF NVA  G K+IY +D++RF+ +DE LKT+SLFEGA+E  RISKS+LKNWVVNA
Sbjct: 604 LAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNA 663

Query: 719 FRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVA 778
           FRERRALALTLNDTKTAV++LH MVN++ GI+IL++WLI+LGI S+KF + +SSQ+VVVA
Sbjct: 664 FRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVA 723

Query: 779 FIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPN 838
           FIFGN CK +FE+II+LFV+HPFDVGDRCEIDG+QMVVEEMNILTTVFLR+DN KV+ PN
Sbjct: 724 FIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPN 783

Query: 839 SVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVF 898
           S+L TK I N+YRSPDMG+ IEF +HI TPAEKI  +K RI SYIEG K+HWYPAPMIVF
Sbjct: 784 SLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVF 843

Query: 899 KDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRS 956
           KD++ LN V++AVW +HRMNHQD GE+WARRS LVEE+ K+C+ELDI+YRL P+DIN+R+
Sbjct: 844 KDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINVRN 853

BLAST of CsGy7G001070 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 891.0 bits (2301), Expect = 8.7e-259
Identity = 487/924 (52.71%), Postives = 653/924 (70.67%), Query Frame = 0

Query: 59  DTSDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDN 118
           D++DR + I+ I+ G  S AV  +  S+   GGN+W+ES YDFW+ ++   G++ + GD+
Sbjct: 5   DSTDRRDFIVNIN-GQESGAVGATGSSSNAEGGNIWKESSYDFWDGEK---GKNDKKGDD 64

Query: 119 SGVDRNEGFEFVQTG------YGMEDPPTKLIGDFLRKQKIEG-ETTLDMDLEMEELKPN 178
              D    F F Q G        + DPP+KLIG FL KQ+  G E +LD++L M EL+ N
Sbjct: 65  EDED-GGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSN 124

Query: 179 RIVPPLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQP 238
              PP   +  +   + L        T IS +   ++ + +             + R++ 
Sbjct: 125 --TPPRPATASNTPRRGL--------TTISESSSPVKTKVK-----------ADAVRRRQ 184

Query: 239 HHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPA---- 298
           +   LG  +    +N   AE ++C S           +K  L R KTKSRL DPP     
Sbjct: 185 NRTSLGGSSDEEGRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTHP 244

Query: 299 ---EPDRLSGLIPKSGQLRSGFLGKI------------EDDDDDPFLEDDLPDEFKRGNF 358
              + +  SG   +SG  +SGFLGK             E++++DPFL++DLP+EFKR   
Sbjct: 245 AIDKTEMKSG--RRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKL 304

Query: 359 SALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRI 418
           S    L+W+SL++I  +L+C+L++  L+ K+ W+LD+WKWEV + +LICGRLVS W +RI
Sbjct: 305 SFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRI 364

Query: 419 GVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVS 478
            VF +E+NF  RKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+K+VE++T ++ L YV+
Sbjct: 365 IVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVT 424

Query: 479 RVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKN 538
           RVLVCLL++ +IWLVKT++VKVLASSFH+STYFDRIQESLF QYVIETLSGPPL+EI++ 
Sbjct: 425 RVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRM 484

Query: 539 EEEEERIADEVQKLQN-AGIIIPPDLKAATFASIKSGREIGSGRTHKSFCAKSCKLSRAL 598
           EEEE+++A++V+ L+  AG  +PP LK    A++KS  ++G          KS  L+R  
Sbjct: 485 EEEEQQVAEDVKSLEKLAGAKLPPALK----ATVKSFMKVG----------KSPGLNRIG 544

Query: 599 TKNRND--GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEI 658
           +K   D  GI ID L +++TKNVSAWNMKRL+NI+  G+ISTLD+ ++    +DE  T I
Sbjct: 545 SKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHI 604

Query: 659 KSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSAL 718
           +SE EAK AA+KIF NV   G +YIYL+D +RF+ E+E  + M+LFEGA+E+ +ISKS L
Sbjct: 605 RSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCL 664

Query: 719 KNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLS 778
           KNWVV AFRERRALALTLNDTKTAVD+LH ++NV+ GI+I+I+WL++LGIA+++F + LS
Sbjct: 665 KNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLS 724

Query: 779 SQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDN 838
           SQ+++VAF+FGN+CKTIFEAIIFLFVMHPFDVGDRCEIDG+Q+VVEEMNILTTVFLRYDN
Sbjct: 725 SQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDN 784

Query: 839 LKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWY 898
            K+I PNSVL TK I N+YRSPDMG+++EF VHIATP EKITA+K RI+SY++  K++WY
Sbjct: 785 QKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWY 844

Query: 899 PAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLP 951
           PAPMIVF  +D LN VK+AVWL+HRMNHQD GER+ RR +L+EEV K C+ELDI+YRL P
Sbjct: 845 PAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYP 875

BLAST of CsGy7G001070 vs. TAIR 10
Match: AT1G53470.1 (mechanosensitive channel of small conductance-like 4 )

HSP 1 Score: 847.4 bits (2188), Expect = 1.1e-245
Identity = 461/922 (50.00%), Postives = 635/922 (68.87%), Query Frame = 0

Query: 60  TSDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNS 119
           T  R + +++IDG                +    WRES  +FW+ND+          D S
Sbjct: 6   TDQRRDFVVRIDG------------EDNGDSEKFWRESSINFWHNDKSSKPPGGEEDDGS 65

Query: 120 GVDRNEGFEFVQTGYGME---DPPTKLIGDFLRKQKIEG-ETTLDMDLEMEELKPNRIVP 179
                  F+F++         DPP+KLI  FL KQK  G E +LDM+  M EL+ N  VP
Sbjct: 66  -------FDFMRRSSEKSEEPDPPSKLINQFLNKQKASGDEISLDMEANMPELQKN-TVP 125

Query: 180 PLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHER 239
           PL+ + +S ++  +       S    + D   RR+NR +  L    K G S   + +   
Sbjct: 126 PLSSTAVSGSASPVTAPVT-ASYRNGTGDAIRRRQNRVT--LSPSVKDGDSSEDEENR-- 185

Query: 240 LGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAE--PDRLS 299
                + G      +E ++C SN       S  R   L++ KT+SRL+DPP    PD +S
Sbjct: 186 -----VDG------SEVVKCTSN------RSTMRTKTLMKMKTRSRLMDPPTPTYPDMVS 245

Query: 300 GLIPKSGQLRSGFLG-------------KIEDDDDDPFLEDDLPDEFKRGNFSALTVLQW 359
           G  P+SG L  GF G             K  ++++DPF E+DLP+  ++       +++W
Sbjct: 246 GRTPRSGNLNPGFSGRNTKPGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEW 305

Query: 360 VSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERN 419
           + LI+I A+LIC+L +PYLR K+LW+L +WKWEVM+ +LICGRLVS W +++ V+F+E N
Sbjct: 306 IFLILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESN 365

Query: 420 FLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLI 479
           FL RK+VLYFVYG+RKPVQNCLWLGLVLIAWH LF+K+VE++  +++L YV++VL+CLL+
Sbjct: 366 FLWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLV 425

Query: 480 STLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIA 539
           + +IWL+KTL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPP +EI     EEE++A
Sbjct: 426 AVIIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEI---HIEEEKVA 485

Query: 540 DEVQKLQNAGIIIPPDLKAATFASIKSGREIGSGRTHK--SFCAKSCKLSRALTKNR--N 599
           ++V+  +  G  + P        S      +GSGR  K  S   KS  LSR+ +K     
Sbjct: 486 NDVKTFEIVGRKLSP--LGPKAVSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGE 545

Query: 600 DGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCL-DDESTTEIKSEREA 659
           +GI IDHL +++TKNVSAW MK+L+N+++ G++STLDEQI+     +D+  T+I+SE EA
Sbjct: 546 EGIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEA 605

Query: 660 KAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVN 719
           K AA+KIFQNVA  G +YIY++D +RF+ EDE  + M LFEGA+E  +ISKS LKNWVVN
Sbjct: 606 KLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVN 665

Query: 720 AFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVV 779
           AFRERRALALTLNDTKTAV++LH +V+V+  I+ILI+WL++LGIA++KF + +SSQ+++V
Sbjct: 666 AFRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLV 725

Query: 780 AFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIP 839
            F+FGN+CKTIFEA+IF+FVMHPFDVGDRCEIDG+QM+VEEMNILTTVFLR+DN K++ P
Sbjct: 726 VFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYP 785

Query: 840 NSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIV 899
           NS+L TK I N+YRSPDM ++IEF VHIATP EK TA++ RI+SY++  K+HW+P+PMIV
Sbjct: 786 NSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIV 845

Query: 900 FKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIR 956
           F+D+ GLN VK+A+W +H+MNHQ+ GER+ RR  L+EE+ ++C+ELDI+YRL P++IN++
Sbjct: 846 FRDMCGLNSVKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVK 880

BLAST of CsGy7G001070 vs. TAIR 10
Match: AT3G14810.2 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 820.8 bits (2119), Expect = 1.1e-237
Identity = 461/924 (49.89%), Postives = 620/924 (67.10%), Query Frame = 0

Query: 59  DTSDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDN 118
           D++DR + I+ I+ G  S AV  +  S+   GGN+W+ES YDFW+ ++   G++ + GD+
Sbjct: 5   DSTDRRDFIVNIN-GQESGAVGATGSSSNAEGGNIWKESSYDFWDGEK---GKNDKKGDD 64

Query: 119 SGVDRNEGFEFVQTG------YGMEDPPTKLIGDFLRKQKIEG-ETTLDMDLEMEELKPN 178
              D    F F Q G        + DPP+KLIG FL KQ+  G E +LD++L M EL+ N
Sbjct: 65  EDED-GGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSN 124

Query: 179 RIVPPLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQP 238
              PP   +  +   + L        T IS +   ++ + +             + R++ 
Sbjct: 125 --TPPRPATASNTPRRGL--------TTISESSSPVKTKVK-----------ADAVRRRQ 184

Query: 239 HHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPA---- 298
           +   LG  +    +N   AE ++C S           +K  L R KTKSRL DPP     
Sbjct: 185 NRTSLGGSSDEEGRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTHP 244

Query: 299 ---EPDRLSGLIPKSGQLRSGFLGKI------------EDDDDDPFLEDDLPDEFKRGNF 358
              + +  SG   +SG  +SGFLGK             E++++DPFL++DLP+EFKR   
Sbjct: 245 AIDKTEMKSG--RRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKL 304

Query: 359 SALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRI 418
           S    L+W+SL++I  +L+C+L++  L+ K+ W+LD+WKWEV + +LICGRLVS W +RI
Sbjct: 305 SFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRI 364

Query: 419 GVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVS 478
            VF +E+NF  RKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+K+VE++T ++ L    
Sbjct: 365 IVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALR--- 424

Query: 479 RVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKN 538
                                         TYFDRIQESLF QYVIETLSGPPL+EI++ 
Sbjct: 425 ------------------------------TYFDRIQESLFTQYVIETLSGPPLMEIQRM 484

Query: 539 EEEEERIADEVQKLQN-AGIIIPPDLKAATFASIKSGREIGSGRTHKSFCAKSCKLSRAL 598
           EEEE+++A++V+ L+  AG  +PP LK    A++KS  ++G          KS  L+R  
Sbjct: 485 EEEEQQVAEDVKSLEKLAGAKLPPALK----ATVKSFMKVG----------KSPGLNRIG 544

Query: 599 TKNRND--GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEI 658
           +K   D  GI ID L +++TKNVSAWNMKRL+NI+  G+ISTLD+ ++    +DE  T I
Sbjct: 545 SKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHI 604

Query: 659 KSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSAL 718
           +SE EAK AA+KIF NV   G +YIYL+D +RF+ E+E  + M+LFEGA+E+ +ISKS L
Sbjct: 605 RSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCL 664

Query: 719 KNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLS 778
           KNWV  AFRERRALALTLNDTKTAVD+LH ++NV+ GI+I+I+WL++LGIA+++F + LS
Sbjct: 665 KNWV--AFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLS 724

Query: 779 SQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDN 838
           SQ+++VAF+FGN+CKTIFEAIIFLFVMHPFDVGDRCEIDG+Q+VVEEMNILTTVFLRYDN
Sbjct: 725 SQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDN 784

Query: 839 LKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWY 898
            K+I PNSVL TK I N+YRSPDMG+++EF VHIATP EKITA+K RI+SY++  K++WY
Sbjct: 785 QKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWY 840

Query: 899 PAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLP 951
           PAPMIVF  +D LN VK+AVWL+HRMNHQD GER+ RR +L+EEV K C+ELDI+YRL P
Sbjct: 845 PAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYP 840

BLAST of CsGy7G001070 vs. TAIR 10
Match: AT2G17000.1 (Mechanosensitive ion channel family protein )

HSP 1 Score: 782.3 bits (2019), Expect = 4.3e-226
Identity = 399/689 (57.91%), Postives = 532/689 (77.21%), Query Frame = 0

Query: 269 RKSNLL-RAKTKSRLIDPPAEPD-RLSGLIPKSGQLRSGFLGKIEDD---DDDPFLEDDL 328
           RK+ L+ RAK +SRLIDPP E + + S  I  S QLRSG LG+  DD   +DD   E+D+
Sbjct: 161 RKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSAEEDV 220

Query: 329 PDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIFILICGR 388
           P E+++    A+T+LQW+SLI +  AL+ +L +   R  +LW L +WKWEV++ +LICGR
Sbjct: 221 PVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGR 280

Query: 389 LVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQT 448
           LVSG GIRI VFFIERNFLLRKRVLYFVYGV+  VQNCLWLGLVL+AWH LF+K+VEK+T
Sbjct: 281 LVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKET 340

Query: 449 NTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSG 508
            + +L  +S++LVC L+ST++WL+KTL+VKVLASSFHVSTYFDRIQE+LF+ Y+IETLSG
Sbjct: 341 QSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLSG 400

Query: 509 PPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAATFASIKSGREIGSGRTHKSFCAK 568
           PP++E+ + EEEE+R  DE+ K+Q  G  + P+L +A F   KSG  +            
Sbjct: 401 PPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTM------------ 460

Query: 569 SCKLSRALTKNRND-GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLD 628
           + K S  + K  +D GIT+D LHK++ KNVSAWNMKRL+ IVR  S+STLDEQ      +
Sbjct: 461 NMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCE 520

Query: 629 DESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQ 688
           DEST +I+SE+EAKAAA+KIF+NVA  G K+IYL+DL+RF+R DE +KTM LFEGA   +
Sbjct: 521 DESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTK 580

Query: 689 RISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASS 748
           +I+KSALKNW+VNAFRERRALALTLNDTKTAV+KLHHM++ +  I+I+++WLI+L IA+S
Sbjct: 581 KITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATS 640

Query: 749 KFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTT 808
           K+ +FL+SQ+V++AF+FGN+ KT+FE+IIFLF++HP+DVGDR  ID ++MVVEEMNILTT
Sbjct: 641 KYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTT 700

Query: 809 VFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIE 868
           VFLR DNLK++ PN +L  K IHN+ RSPDMG+ +   VHI TP EKI A+K RI SYI+
Sbjct: 701 VFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYID 760

Query: 869 GNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELD 928
              E+WYP   ++ KD++ LN V++A+WL H++NHQ+ GER+ RR++L+EEV+K+  ELD
Sbjct: 761 SKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELD 820

Query: 929 IQYRLLPIDINIRSLPSSAPSIGFPSNWT 952
           IQYR  P+DIN++++P+   S   P  W+
Sbjct: 821 IQYRFHPLDINVKTMPTVVSS-RVPPAWS 836