CsGy6G036237 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy6G036237
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionUnknown protein
LocationGy14Chr6: 31847199 .. 31847957 (-)
RNA-Seq ExpressionCsGy6G036237
SyntenyCsGy6G036237
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTTGTCCAAAGTACCAAACAGAAAATAAAGTTTCCGGAAACAAAAAAGAAAAAACAAGATTTTAAAACCGCTTTCCTTTGCTTCTCCTTTACGCCAAATCCCAATCTTCTTCCCATGATTATCCCTAACATCTCTCAATCACTCTCTCCCCTTTCTTATTCCCTATCCCTTCCCTAATTCTCCACTACTACTTTCTAATCCAACCAAATCCCCCCCCACTGGAATCACCCACAATGATTCCCATCAAATCCTCGGCGGCCATCGTCGCCTTCTTCTCACTGATCGCATCGATCGCGGCAGTGTCGGAGATACGCCCGTCAGAGCATGGGTTGGAGTTTCAGAGCCCTCCACCGGTTGGAGACAAATCGTCACCGGAGATGCGATCGTTTTTCGGAGGAATAGCGTCGCCGACACCGGAAGTGGCATTGCCGATACCGAAGACCTTGAATTCGAGTGAATCGCCGGGATGGTGGAACCACCATGACGGTGGAAATAAACGACTGAGAAATGCATTATTGGTGGCGACGGCGGCTTGTGGAATTACAGGTGTCACTTTATTAGTGGGTTCTACGTTATTCTACATTTTTAAGGCCAAAAATAAAAGATCAATGCCGTTATCACCAAATAATAATCACAAATAAAAGTATGATTATCATCATAATCATAATTGATGATAACTTTACACCAAGAATTTAGCTGTCATGAAATAGCCATATTTTTCTTAGTTCCAATTTTTTCTTACCGTTTGAGAATTCAC

mRNA sequence

CTTTTGTCCAAAGTACCAAACAGAAAATAAAGTTTCCGGAAACAAAAAAGAAAAAACAAGATTTTAAAACCGCTTTCCTTTGCTTCTCCTTTACGCCAAATCCCAATCTTCTTCCCATGATTATCCCTAACATCTCTCAATCACTCTCTCCCCTTTCTTATTCCCTATCCCTTCCCTAATTCTCCACTACTACTTTCTAATCCAACCAAATCCCCCCCCACTGGAATCACCCACAATGATTCCCATCAAATCCTCGGCGGCCATCGTCGCCTTCTTCTCACTGATCGCATCGATCGCGGCAGTGTCGGAGATACGCCCGTCAGAGCATGGGTTGGAGTTTCAGAGCCCTCCACCGGTTGGAGACAAATCGTCACCGGAGATGCGATCGTTTTTCGGAGGAATAGCGTCGCCGACACCGGAAGTGGCATTGCCGATACCGAAGACCTTGAATTCGAGTGAATCGCCGGGATGGTGGAACCACCATGACGGTGGAAATAAACGACTGAGAAATGCATTATTGGTGGCGACGGCGGCTTGTGGAATTACAGGTGTCACTTTATTAGTGGGTTCTACGTTATTCTACATTTTTAAGGCCAAAAATAAAAGATCAATGCCGTTATCACCAAATAATAATCACAAATAAAAGTATGATTATCATCATAATCATAATTGATGATAACTTTACACCAAGAATTTAGCTGTCATGAAATAGCCATATTTTTCTTAGTTCCAATTTTTTCTTACCGTTTGAGAATTCAC

Coding sequence (CDS)

ATGATTCCCATCAAATCCTCGGCGGCCATCGTCGCCTTCTTCTCACTGATCGCATCGATCGCGGCAGTGTCGGAGATACGCCCGTCAGAGCATGGGTTGGAGTTTCAGAGCCCTCCACCGGTTGGAGACAAATCGTCACCGGAGATGCGATCGTTTTTCGGAGGAATAGCGTCGCCGACACCGGAAGTGGCATTGCCGATACCGAAGACCTTGAATTCGAGTGAATCGCCGGGATGGTGGAACCACCATGACGGTGGAAATAAACGACTGAGAAATGCATTATTGGTGGCGACGGCGGCTTGTGGAATTACAGGTGTCACTTTATTAGTGGGTTCTACGTTATTCTACATTTTTAAGGCCAAAAATAAAAGATCAATGCCGTTATCACCAAATAATAATCACAAATAA

Protein sequence

MIPIKSSAAIVAFFSLIASIAAVSEIRPSEHGLEFQSPPPVGDKSSPEMRSFFGGIASPTPEVALPIPKTLNSSESPGWWNHHDGGNKRLRNALLVATAACGITGVTLLVGSTLFYIFKAKNKRSMPLSPNNNHK*
Homology
BLAST of CsGy6G036237 vs. NCBI nr
Match: KAE8647762.1 (hypothetical protein Csa_003695 [Cucumis sativus])

HSP 1 Score: 218 bits (555), Expect = 7.58e-71
Identity = 109/110 (99.09%), Postives = 110/110 (100.00%), Query Frame = 0

Query: 1   MIPIKSSAAIVAFFSLIASIAAVSEIRPSEHGLEFQSPPPVGDKSSPEMRSFFGGIASPT 60
           MIPIKSSAAIVAFFSLIASIAAVSEIRPSEHGLEFQSPPPVGDKSSPEMRSFFGGIASPT
Sbjct: 1   MIPIKSSAAIVAFFSLIASIAAVSEIRPSEHGLEFQSPPPVGDKSSPEMRSFFGGIASPT 60

Query: 61  PEVALPIPKTLNSSESPGWWNHHDGGNKRLRNALLVATAACGITGVTLLV 110
           PEVALPIPKTLNSSESPGWWNHHDGGNKRLRNALLVATAACGITGVTLL+
Sbjct: 61  PEVALPIPKTLNSSESPGWWNHHDGGNKRLRNALLVATAACGITGVTLLI 110

BLAST of CsGy6G036237 vs. NCBI nr
Match: XP_023518243.1 (uncharacterized protein LOC111781779 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 213 bits (543), Expect = 9.23e-69
Identity = 106/136 (77.94%), Postives = 117/136 (86.03%), Query Frame = 0

Query: 1   MIPIKSSAAIVAFFSLIASIAAVSEIRPSEHGLEFQSPPPVGDKSSPEMRSFFGGIASPT 60
           MI IKS AA+  +   I +IAA  EIRPSEHGLEFQSPP  GDKSSPEMRSFFGG +SPT
Sbjct: 1   MITIKSMAAMAVYLLFIVAIAAALEIRPSEHGLEFQSPPAAGDKSSPEMRSFFGGTSSPT 60

Query: 61  PEVALPIPKTLNSSESPGWWNHHDGGNKRLRNALLVATAACGITGVTLLVGSTLFYIFKA 120
           PEVALP+PK +NSSE+PGWW H DGG+KRLRNALLVATAACGITGVTLLVGSTL+YIFK 
Sbjct: 61  PEVALPLPKAMNSSEAPGWWTHRDGGDKRLRNALLVATAACGITGVTLLVGSTLYYIFKV 120

Query: 121 KNKRSMPLSPNN-NHK 135
           KN+RS+PLS NN NHK
Sbjct: 121 KNQRSLPLSSNNSNHK 136

BLAST of CsGy6G036237 vs. NCBI nr
Match: XP_023003567.1 (uncharacterized protein LOC111497130 [Cucurbita maxima])

HSP 1 Score: 210 bits (535), Expect = 1.53e-67
Identity = 102/134 (76.12%), Postives = 115/134 (85.82%), Query Frame = 0

Query: 1   MIPIKSSAAIVAFFSLIASIAAVSEIRPSEHGLEFQSPPPVGDKSSPEMRSFFGGIASPT 60
           MI IKS AA+  +   I +I A  EIRPSEHGLEFQSPP  GDKSSPEMRSFFGG +SPT
Sbjct: 1   MITIKSMAAMAVYLLFIVAIEAALEIRPSEHGLEFQSPPAAGDKSSPEMRSFFGGTSSPT 60

Query: 61  PEVALPIPKTLNSSESPGWWNHHDGGNKRLRNALLVATAACGITGVTLLVGSTLFYIFKA 120
           PEVALP+PK +NSSE+PGWW H DGG+KRLRNALLVATAACG+TGVTLLVGSTL+YIFK 
Sbjct: 61  PEVALPLPKAMNSSEAPGWWTHRDGGDKRLRNALLVATAACGMTGVTLLVGSTLYYIFKV 120

Query: 121 KNKRSMPLSPNNNH 134
           KN+RS+PLS NNN+
Sbjct: 121 KNQRSLPLSSNNNN 134

BLAST of CsGy6G036237 vs. NCBI nr
Match: XP_022926736.1 (uncharacterized protein LOC111433768 [Cucurbita moschata])

HSP 1 Score: 209 bits (533), Expect = 3.08e-67
Identity = 105/136 (77.21%), Postives = 116/136 (85.29%), Query Frame = 0

Query: 1   MIPIKSSAAIVAFFSLIASIAAVSEIRPSEHGLEFQSPPPVGDKSSPEMRSFFGGIASPT 60
           MI IKS AA+  +   I +IAA  EIRPSEHGLEFQS P  GDKSSPEMRSFFGG +SPT
Sbjct: 1   MITIKSMAAMAVYLLFIVAIAAALEIRPSEHGLEFQSLPAAGDKSSPEMRSFFGGTSSPT 60

Query: 61  PEVALPIPKTLNSSESPGWWNHHDGGNKRLRNALLVATAACGITGVTLLVGSTLFYIFKA 120
           PEVALP+PK +NSSE+PGWW H DGG+KRLRNALLVATAACGITGVTLLVGSTL+YIFK 
Sbjct: 61  PEVALPLPKAMNSSEAPGWWTHRDGGDKRLRNALLVATAACGITGVTLLVGSTLYYIFKV 120

Query: 121 KNKRSMPLSPNN-NHK 135
           KN+RS+PLS NN NHK
Sbjct: 121 KNQRSLPLSSNNSNHK 136

BLAST of CsGy6G036237 vs. NCBI nr
Match: XP_022925668.1 (uncharacterized protein LOC111433017 [Cucurbita moschata])

HSP 1 Score: 208 bits (530), Expect = 5.51e-66
Identity = 99/135 (73.33%), Postives = 114/135 (84.44%), Query Frame = 0

Query: 1   MIPIKSSAAIVAFFSLIASIAAVSEIRPSEHGLEFQSPPPVGDKSSPEMRSFFGGIASPT 60
           +IPIK  AA+V F  LI ++A+  EIRPSEHGLEFQSPPP G+KSSPEMRSFF G +SP 
Sbjct: 58  IIPIKPVAAMVVFLPLIVAVASALEIRPSEHGLEFQSPPPAGEKSSPEMRSFFVGTSSPI 117

Query: 61  PEVALPIPKTLNSSESPGWWNHHDGGNKRLRNALLVATAACGITGVTLLVGSTLFYIFKA 120
           P+  LP+PK +NSSE+PGWW H DGGNKR+RNALLVATAACGITGVTLLVGSTLFYI+K 
Sbjct: 118 PDTTLPLPKAMNSSEAPGWWTHRDGGNKRVRNALLVATAACGITGVTLLVGSTLFYIYKV 177

Query: 121 KNKRSMPLSPNNNHK 135
           KN+  +PLS NNNHK
Sbjct: 178 KNQTPLPLSSNNNHK 192

BLAST of CsGy6G036237 vs. ExPASy TrEMBL
Match: A0A0A0KM65 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G525480 PE=4 SV=1)

HSP 1 Score: 269 bits (688), Expect = 3.36e-91
Identity = 135/135 (100.00%), Postives = 135/135 (100.00%), Query Frame = 0

Query: 1   MIPIKSSAAIVAFFSLIASIAAVSEIRPSEHGLEFQSPPPVGDKSSPEMRSFFGGIASPT 60
           MIPIKSSAAIVAFFSLIASIAAVSEIRPSEHGLEFQSPPPVGDKSSPEMRSFFGGIASPT
Sbjct: 1   MIPIKSSAAIVAFFSLIASIAAVSEIRPSEHGLEFQSPPPVGDKSSPEMRSFFGGIASPT 60

Query: 61  PEVALPIPKTLNSSESPGWWNHHDGGNKRLRNALLVATAACGITGVTLLVGSTLFYIFKA 120
           PEVALPIPKTLNSSESPGWWNHHDGGNKRLRNALLVATAACGITGVTLLVGSTLFYIFKA
Sbjct: 61  PEVALPIPKTLNSSESPGWWNHHDGGNKRLRNALLVATAACGITGVTLLVGSTLFYIFKA 120

Query: 121 KNKRSMPLSPNNNHK 135
           KNKRSMPLSPNNNHK
Sbjct: 121 KNKRSMPLSPNNNHK 135

BLAST of CsGy6G036237 vs. ExPASy TrEMBL
Match: A0A6J1KMY3 (uncharacterized protein LOC111497130 OS=Cucurbita maxima OX=3661 GN=LOC111497130 PE=4 SV=1)

HSP 1 Score: 210 bits (535), Expect = 7.40e-68
Identity = 102/134 (76.12%), Postives = 115/134 (85.82%), Query Frame = 0

Query: 1   MIPIKSSAAIVAFFSLIASIAAVSEIRPSEHGLEFQSPPPVGDKSSPEMRSFFGGIASPT 60
           MI IKS AA+  +   I +I A  EIRPSEHGLEFQSPP  GDKSSPEMRSFFGG +SPT
Sbjct: 1   MITIKSMAAMAVYLLFIVAIEAALEIRPSEHGLEFQSPPAAGDKSSPEMRSFFGGTSSPT 60

Query: 61  PEVALPIPKTLNSSESPGWWNHHDGGNKRLRNALLVATAACGITGVTLLVGSTLFYIFKA 120
           PEVALP+PK +NSSE+PGWW H DGG+KRLRNALLVATAACG+TGVTLLVGSTL+YIFK 
Sbjct: 61  PEVALPLPKAMNSSEAPGWWTHRDGGDKRLRNALLVATAACGMTGVTLLVGSTLYYIFKV 120

Query: 121 KNKRSMPLSPNNNH 134
           KN+RS+PLS NNN+
Sbjct: 121 KNQRSLPLSSNNNN 134

BLAST of CsGy6G036237 vs. ExPASy TrEMBL
Match: A0A6J1EFQ9 (uncharacterized protein LOC111433768 OS=Cucurbita moschata OX=3662 GN=LOC111433768 PE=4 SV=1)

HSP 1 Score: 209 bits (533), Expect = 1.49e-67
Identity = 105/136 (77.21%), Postives = 116/136 (85.29%), Query Frame = 0

Query: 1   MIPIKSSAAIVAFFSLIASIAAVSEIRPSEHGLEFQSPPPVGDKSSPEMRSFFGGIASPT 60
           MI IKS AA+  +   I +IAA  EIRPSEHGLEFQS P  GDKSSPEMRSFFGG +SPT
Sbjct: 1   MITIKSMAAMAVYLLFIVAIAAALEIRPSEHGLEFQSLPAAGDKSSPEMRSFFGGTSSPT 60

Query: 61  PEVALPIPKTLNSSESPGWWNHHDGGNKRLRNALLVATAACGITGVTLLVGSTLFYIFKA 120
           PEVALP+PK +NSSE+PGWW H DGG+KRLRNALLVATAACGITGVTLLVGSTL+YIFK 
Sbjct: 61  PEVALPLPKAMNSSEAPGWWTHRDGGDKRLRNALLVATAACGITGVTLLVGSTLYYIFKV 120

Query: 121 KNKRSMPLSPNN-NHK 135
           KN+RS+PLS NN NHK
Sbjct: 121 KNQRSLPLSSNNSNHK 136

BLAST of CsGy6G036237 vs. ExPASy TrEMBL
Match: A0A6J1EFW4 (uncharacterized protein LOC111433017 OS=Cucurbita moschata OX=3662 GN=LOC111433017 PE=4 SV=1)

HSP 1 Score: 208 bits (530), Expect = 2.67e-66
Identity = 99/135 (73.33%), Postives = 114/135 (84.44%), Query Frame = 0

Query: 1   MIPIKSSAAIVAFFSLIASIAAVSEIRPSEHGLEFQSPPPVGDKSSPEMRSFFGGIASPT 60
           +IPIK  AA+V F  LI ++A+  EIRPSEHGLEFQSPPP G+KSSPEMRSFF G +SP 
Sbjct: 58  IIPIKPVAAMVVFLPLIVAVASALEIRPSEHGLEFQSPPPAGEKSSPEMRSFFVGTSSPI 117

Query: 61  PEVALPIPKTLNSSESPGWWNHHDGGNKRLRNALLVATAACGITGVTLLVGSTLFYIFKA 120
           P+  LP+PK +NSSE+PGWW H DGGNKR+RNALLVATAACGITGVTLLVGSTLFYI+K 
Sbjct: 118 PDTTLPLPKAMNSSEAPGWWTHRDGGNKRVRNALLVATAACGITGVTLLVGSTLFYIYKV 177

Query: 121 KNKRSMPLSPNNNHK 135
           KN+  +PLS NNNHK
Sbjct: 178 KNQTPLPLSSNNNHK 192

BLAST of CsGy6G036237 vs. ExPASy TrEMBL
Match: A0A5D3CP09 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009600 PE=4 SV=1)

HSP 1 Score: 198 bits (503), Expect = 2.05e-63
Identity = 97/104 (93.27%), Postives = 101/104 (97.12%), Query Frame = 0

Query: 1   MIPIKSSAAIVAFFSLIASIAAVSEIRPSEHGLEFQSPPPVGDKSSPEMRSFFGGIASPT 60
           MIPIKS+AAIVAFFSLIA+IAAVSEIRPSEHGLEFQSPPP G+KSSPEMRSFFGGI SPT
Sbjct: 1   MIPIKSAAAIVAFFSLIAAIAAVSEIRPSEHGLEFQSPPPAGEKSSPEMRSFFGGITSPT 60

Query: 61  PEVALPIPKTLNSSESPGWWNHHDGGNKRLRNALLVATAACGIT 104
           PEVALPIPKT+NSSESPGWWNH DGGNKRLRNALLVATAACGIT
Sbjct: 61  PEVALPIPKTMNSSESPGWWNHRDGGNKRLRNALLVATAACGIT 104

BLAST of CsGy6G036237 vs. TAIR 10
Match: AT4G21740.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G30515.1); Has 20 Blast hits to 20 proteins in 4 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 20; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 69.3 bits (168), Expect = 2.7e-12
Identity = 47/133 (35.34%), Postives = 69/133 (51.88%), Query Frame = 0

Query: 11  VAFFSLIASIAAVSEIRPSEHGLEFQ-SPPPVGDKSSP-EMRSFFGGIASPTPEVALP-- 70
           + F  +    +   E+RPS+HGL++Q S PP    S P +M+SFFG   S +P  + P  
Sbjct: 20  ITFLVIFTGNSLAGELRPSDHGLQYQFSSPPTESHSPPGKMKSFFGDSHSSSPPPSHPQL 79

Query: 71  IPK--TLNSSESPGWWNHHDGGNKR----LRNALLVATAACGITGVTLLVGSTLFYIFKA 130
           +PK    +  +   WW   DG   R    +R+  L A+  CG++GV LLV  TL Y F+ 
Sbjct: 80  LPKATAADGGDDDSWW--RDGAGIRRDHVMRHVFLAASIICGVSGVALLVVFTLIYFFRY 139

Query: 131 KNKRSMPLSPNNN 134
           + K +   SP  N
Sbjct: 140 R-KHNHSNSPTGN 149

BLAST of CsGy6G036237 vs. TAIR 10
Match: AT1G30515.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 16 plant structures; EXPRESSED DURING: 6 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G21740.1); Has 20 Blast hits to 20 proteins in 4 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 20; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 49.7 bits (117), Expect = 2.2e-06
Identity = 40/113 (35.40%), Postives = 58/113 (51.33%), Query Frame = 0

Query: 7   SAAIVAFFSLIASIAAVSEIRPSEHGLEFQSPPPVGDKSSPEMRSFFGGIAS-PTPEVAL 66
           S  I+    L ++I    E+RPS+HGLE+   P      S EM SFFG  +S     ++ 
Sbjct: 14  SMLIMFIIVLESTIINARELRPSDHGLEYYYEP----GESSEMTSFFGPPSSNDLTSISS 73

Query: 67  PIPKTLNSS-ESPGWWNHHDGGNKRLRN-ALLVATAACGITGVTLLVGSTLFY 117
           P    L S+ +SP      D  + R+ N  L+V +  CG++GV L+V S L Y
Sbjct: 74  PSSSILPSAVKSPMKTLSKDQDDDRVMNHVLVVGSLVCGLSGVALMVASALIY 122

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
KAE8647762.17.58e-7199.09hypothetical protein Csa_003695 [Cucumis sativus][more]
XP_023518243.19.23e-6977.94uncharacterized protein LOC111781779 [Cucurbita pepo subsp. pepo][more]
XP_023003567.11.53e-6776.12uncharacterized protein LOC111497130 [Cucurbita maxima][more]
XP_022926736.13.08e-6777.21uncharacterized protein LOC111433768 [Cucurbita moschata][more]
XP_022925668.15.51e-6673.33uncharacterized protein LOC111433017 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0KM653.36e-91100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G525480 PE=4 SV=1[more]
A0A6J1KMY37.40e-6876.12uncharacterized protein LOC111497130 OS=Cucurbita maxima OX=3661 GN=LOC111497130... [more]
A0A6J1EFQ91.49e-6777.21uncharacterized protein LOC111433768 OS=Cucurbita moschata OX=3662 GN=LOC1114337... [more]
A0A6J1EFW42.67e-6673.33uncharacterized protein LOC111433017 OS=Cucurbita moschata OX=3662 GN=LOC1114330... [more]
A0A5D3CP092.05e-6393.27Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
Match NameE-valueIdentityDescription
AT4G21740.12.7e-1235.34unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G30515.12.2e-0635.40unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR37189:SF4CONCANAVALIN A-LIKE LECTIN/GLUCANASE DOMAIN-CONTAINING PROTEIN-RELATEDcoord: 5..129
NoneNo IPR availablePANTHERPTHR37189CONCANAVALIN A-LIKE LECTIN/GLUCANASE DOMAIN-CONTAINING PROTEIN-RELATEDcoord: 5..129

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy6G036237.1CsGy6G036237.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane