CsGy6G034860 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy6G034860
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionUbiquitin-like modifier-activating enzyme ATG7
LocationGy14Chr6: 30916146 .. 30920912 (-)
RNA-Seq ExpressionCsGy6G034860
SyntenyCsGy6G034860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAGACGGGAATCTACTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTACTTCTTCTTCAAGAATTCCTCCTCACCGGCATCTATACAGAAATTTGAGCCACAAGAACAAGAATATGGCCTACGATTACGGATCTATATTGCAATTCGCACCACTTCAAAGCGCTGTCGACGAAGGGTTCTGGCATAGACTCTCTTCCTTGAAGCTCAACCAGCTAGGCATCGACGATTCCCCTATCCCCATCACTGGTCTGCTTCTCTCTCTCTCTCGCTCTTTCTTTCTTTCTTTCTTTCTTTCTTCCTTCCTTTCTTCCTTTCTTTCTTTCTTCTATGTAATTCACGATTACTAGCCACCAGCAACTATCCTGTTCTTGGTTTTTTGGATTTCTTTTATATGATCTTATGACCAAAATTAAGTTTCTGGCGATCAGTAAAACTCAAACCCTTGAACCAGGAAAGAATGTTGTTGATCAAACGGAGTAGTATATTTAATGAGTTTTGAGTTTGAATTTGTATAATTTGTTTTGGCATCCTAAGATTCTAACTCCTAAAGTACATGGTTGGTTTTGTTTATATTTTGTTATTTTTCTCTGCAATCAGAAATGGAAATAAAAGATACATAACTATGATCACTATCTGTTGTTCATGCACATAAACAAGATAATCAAGAACCAACTCCTTGTAGGTTTCTTTGCTCCTTGTTCACATTCCCTACTGTCCAATCATTTGACACTTCTATCTGAGTCATTGCCTATCGAAGTAAGGAGGGACTCATCGACACCATTAACTACTAAAGGCAATAGGAATAGGTGCGCTGTTCCTGGAATTTTATACAATACTAATACTGTAGAGAGCTTCCATGCTCTTGACAGGCTTAGCTTACTAAAGTCTGAAGCAAATAAGGTATATTATCTCAAATGATTTTCATATCTTGTTACAGTACACGGGCATTGGAAATTCCAATTTCTTGTTTATAGTAAGATCAGTTTTCCGTTGTTCTGAATAGATTTGGGAGGATATTCGCAGCGGCAAAGCTTTAGAGGATAGTTCAGTACTTGCAAGGTTCCTTTTAATCTCATTCGCAGACTTGAAAATATGGAACTTTCACTATTGCTTTGCTTTCCCTGCTCTTGTTCTTGATCCTCCAGCAACTGTAGTTGGACTGACATCTGCTTCACAGTGGTTTAACTTCAAAGAGGTATGCTGGATTTTTATCATAATCTATATTATGTTGTTCAAGAGTCGAAGAGATGTTTTGATTTTTCTCTCTACTTTTTGTTTCATTGCTTAGGCTGAATCATTGTTCACAGCTTTTGGTCAGTGGCGCAGCTCAGATCTAACTTCTGGTAGGCTTGCATTCCTTCACTGTTCGGAGTTTTGTAAATTAAAAGTCAATCTTTGGTTCATTACAGTTTTTTTTGCATCCTACTGCAGACATACCTTTTTTCTTGGTGAATATTGATTCCAGTTCTCAAGCTAGCATTAAGCATCTGAGAGATTTTGAAACCTGCCAAAACAATGGGGGAAAGGTCTGTTGGGGTTACAACTCTAGTCAGTGAGGAAGTAGCCAAATTGTCTTTCAAGTCTTTACTACAGAGATAGTTTGGTGATTCATAGTGTTATGAAGTCCCATGACCATAAAATTATTAATGAAACTTTCTTATAGCTAGCTTAGTCTTTTTTTGCAGCTACTTTTTGGCTTTTATGACCCTTGTCATCTTCCAAGTAATCCCGGCTGGCCTCTTCGCAACTTCTTAGCTCTGATTTATTCGAAGTGGAATCTGAAGTCAGTTGACTTTTTATGCTATAGAGAGAACCGTGGTTTTGCTGATCTTAGGTTATCCCTTGTTGGTACAGCCCTGATTGATGATCCCAAAGGTACTAGAAGAAATTTGTAACAGCACGAGCTTTTTCTCTACTCCTTTGCAAAGTAGCGCAGCTAACTCTCTGTCTCTCTGTCTCTCTCTTTCTACAGGGTTCAGAGATCCATCCTGTATGCCTAATCCGGTTGGATGGGAACTGAAAAGAGGGAAGAAATTCTACAAAACTATCAACCTTGCAAAATCCATGGACCCAACTCGGTATATTTGTATGCACCTAGCATTTTAATGGAGGAAGATGAGGTCCAGTTATTAAGTAATATGTACTTTCTTGACAGGTTAGCTATATCAGCAGCAGATTTGAATTTGAGACTAATGAGGTGGCGTGCTTTGCCTTCTTTAAACATAAACATGTTGTCTTCTCTCAAGTGTCTCTTGCTAGGGGCAGGTACTCTTGGCTGTCAGGTTGCTCGTATGCTTATGGTGAGTGCCTTCTAGAAAGTTGTGGTCAAGTTTCAATCTATTCCTTAACAAAGGTACCTTGTAGGCATGGGGTGTACGAAAAATTACACTAGTGGACAGTGGAAGGGTAGCTATGTCTAATCCATTGAGGCAATCACTTTACACATTGGAGGACTGCCTTAATGGTGGTAACTTTAAAGCTGAAGCAGCAGTTAAAAGTCTCAATCGAATTTTTCCCGCCATGGTAAAATTTGATGCATGACCCCAATACAGAAAACTGCCTTATTTTGTCTTCTATTTCTTTCTTGCTTCTTCCTTTTTAATTATTAATAAAAAATACCTAGGAAGCTGAAGGTGTTGTGATTTCCATACCAATGCCTGGGCATCCTGTTCCAGACCATGAAGCAGCCAGCACAATTGATGATTGCAGACGACTTGATGACTTAATTAATTCTCATGATGCAATTTTCTTGTTGACTGATACTAGGGAAAGTAGGTGGCTTCCTACACTTCTATGTGCCAATGCAAACAAGGTATATTTTTGTCGTACAAGTTGTGTGCATAGGTTTCTCATACTTGGTGTCTCAATTTAATGTCTGCTTCTGATTTGAAGGTTACTATCACCGCAGCATTGGGGTTTGACAGTTTCTTAGTTATGCGCCATGGAGCTGGTCCTTCTGGTTCCTGGCATGACTCCACATCTCAAACTAATATAGCTAACCTTTCCTTAAATACTACAAACACGAGGCAGAGATTGGGCTGTTACTTCTGTAATGATGTTGTTGCGCCTATTGATGTAATATATCTAAAGCTCACGTGATGTATTTTGGTTGATTTATTTGTTTTGTGTTTATTCTCGAGTTACATGCTGTTCTTGCAGTCAACTGCCAATCGGACATTGGACCAGCAGTGCACTGTTACTCGCCCAGGGCTTGCTCCCATTGCATCAGCGATTGCAGTTGAACTTCTTGTTGGAATTTTACATCATCCTGAAGGGTAAGTCCTCGCAGACAGTGGTGATAAAGGCTTGAGATTTCTTTGTGGCTTGACTACTGAAAAGATGCTTAGGTGTGATAGTTGAAGTGGAGTTAATTGAGGGTTCAAAGTTCAAACTGAGTTTCAAAGGGGCTTGAGCATGTCGTATTGATCTTTACTCTAAACTGTGCAGAATATATGCTGAAGGTGAACTTTTGAACTCTGGGATTGCTGGAGCTTCTGAGCAGCCTCTTGGAATCCTGCCTCATCAAATTCGAGGTTTCTTCTCACAGTTTTCTCAGATGACACTTGTTGGCCGCTCATCAGATAGTTGTACAGCTTGCTCCAGCATGGTAAGTTATGCTGCTCATTTGTCCCATATTTTCTTATTTGTTCCTTTGCACTTAACATCTTTTAATTTAAATTTCTTATTTCACTTCGGGTTGAGCATTGACGCTTAACAATTCAACTTCATATTAATTCGAATACTTGAATACTTCAAACAGATTTTATTTTAATAAGTACTTACAGCAAATGTCTATGTGAGAACTTCTTCAAACAATATCATTGCTCACCTAAACTCTCCAATCGTACCTATTCAAAGAATTATTGAATTTATCTGAAGATGCAAGGGCTATAGCTCAAGTCCTTTCAGAATACAGCCGTGTAGAAACAATGATATCAAATATGTAGAGTGTTTTAAGTTTTAGAACCGCTGAATTGTGCAATATGATGATTCAATAATAGTCTAGAAAAACGGGGAGAGAGTGTAAGCCTAAATCTGTTAGAAGTTTCTTCTCGCATTTTGCCTAACCTGTTTCGTTTTGACGAAGATTAATATCCTTGAATTTTCTTGGGTTAGTTTCCCTTCCTTTTAATTTCAAAATACGGTGGAATTGTGCAGGTTGTATCTGAATACCGAAATAGGGGGATGGATTTTATTCTTCAAGCGATTAACCATCCAACTTATTTGGAGGATTTGACCGGACTTACTGAGTTAATGAAGTCAACTAGCTCTTTTCAATTGGACTGGGACAATGACTCAGATGGTAGTGGTGATGATGATGGATGTATTGAAATTTGATACAAACTTTCATCTTGCTTGTATACAAATTACATCAATTCAACAACATTTTTTTGTACATAAATACTGAACAAATGAAATTCCAAATTTGGTATGTCTTCAGTTTTACTTGGTAGGTAAAAATCTGGTTGCTTCCAGATCGCTGAATACATAACTTTCTCTGGTATTTTGTTTATTATCGTTTTAATGCTCGGTGATGTGCATAGATAATATATCAAAGATGAAATTAATATTTTAACATATTACATTAATAAACAGAACAACACAACCCAACGAAATGTCCACTTTTTACGATACCTTTTAGGTGCCTCTGCCCATAAGGCCCAATGGAAATCGATTTGTTTGTCCTTGGAATTGATAAAAACGCAACTTGTTCTCAACTAGCACCAGTGGTCTAGTGGTAGAATAGTACCCTGCCAC

mRNA sequence

TAAGACGGGAATCTACTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTACTTCTTCTTCAAGAATTCCTCCTCACCGGCATCTATACAGAAATTTGAGCCACAAGAACAAGAATATGGCCTACGATTACGGATCTATATTGCAATTCGCACCACTTCAAAGCGCTGTCGACGAAGGGTTCTGGCATAGACTCTCTTCCTTGAAGCTCAACCAGCTAGGCATCGACGATTCCCCTATCCCCATCACTGGTTTCTTTGCTCCTTGTTCACATTCCCTACTGTCCAATCATTTGACACTTCTATCTGAGTCATTGCCTATCGAAGTAAGGAGGGACTCATCGACACCATTAACTACTAAAGGCAATAGGAATAGGTGCGCTGTTCCTGGAATTTTATACAATACTAATACTGTAGAGAGCTTCCATGCTCTTGACAGGCTTAGCTTACTAAAGTCTGAAGCAAATAAGATTTGGGAGGATATTCGCAGCGGCAAAGCTTTAGAGGATAGTTCAGTACTTGCAAGGTTCCTTTTAATCTCATTCGCAGACTTGAAAATATGGAACTTTCACTATTGCTTTGCTTTCCCTGCTCTTGTTCTTGATCCTCCAGCAACTGTAGTTGGACTGACATCTGCTTCACAGTGGTTTAACTTCAAAGAGGCTGAATCATTGTTCACAGCTTTTGGTCAGTGGCGCAGCTCAGATCTAACTTCTGACATACCTTTTTTCTTGGTGAATATTGATTCCAGTTCTCAAGCTAGCATTAAGCATCTGAGAGATTTTGAAACCTGCCAAAACAATGGGGGAAAGCTACTTTTTGGCTTTTATGACCCTTGTCATCTTCCAAGTAATCCCGGCTGGCCTCTTCGCAACTTCTTAGCTCTGATTTATTCGAAGTGGAATCTGAAGTCAGTTGACTTTTTATGCTATAGAGAGAACCGTGGTTTTGCTGATCTTAGGTTATCCCTTGTTGGTACAGCCCTGATTGATGATCCCAAAGGGTTCAGAGATCCATCCTGTATGCCTAATCCGGTTGGATGGGAACTGAAAAGAGGGAAGAAATTCTACAAAACTATCAACCTTGCAAAATCCATGGACCCAACTCGGTTAGCTATATCAGCAGCAGATTTGAATTTGAGACTAATGAGGTGGCGTGCTTTGCCTTCTTTAAACATAAACATGTTGTCTTCTCTCAAGTGTCTCTTGCTAGGGGCAGGTACTCTTGGCTGTCAGGTTGCTCGTATGCTTATGGCATGGGGTGTACGAAAAATTACACTAGTGGACAGTGGAAGGGTAGCTATGTCTAATCCATTGAGGCAATCACTTTACACATTGGAGGACTGCCTTAATGGTGGTAACTTTAAAGCTGAAGCAGCAGTTAAAAGTCTCAATCGAATTTTTCCCGCCATGGAAGCTGAAGGTGTTGTGATTTCCATACCAATGCCTGGGCATCCTGTTCCAGACCATGAAGCAGCCAGCACAATTGATGATTGCAGACGACTTGATGACTTAATTAATTCTCATGATGCAATTTTCTTGTTGACTGATACTAGGGAAAGTAGGTGGCTTCCTACACTTCTATGTGCCAATGCAAACAAGGTTACTATCACCGCAGCATTGGGGTTTGACAGTTTCTTAGTTATGCGCCATGGAGCTGGTCCTTCTGGTTCCTGGCATGACTCCACATCTCAAACTAATATAGCTAACCTTTCCTTAAATACTACAAACACGAGGCAGAGATTGGGCTGTTACTTCTGTAATGATGTTGTTGCGCCTATTGATTCAACTGCCAATCGGACATTGGACCAGCAGTGCACTGTTACTCGCCCAGGGCTTGCTCCCATTGCATCAGCGATTGCAGTTGAACTTCTTGTTGGAATTTTACATCATCCTGAAGGAATATATGCTGAAGGTGAACTTTTGAACTCTGGGATTGCTGGAGCTTCTGAGCAGCCTCTTGGAATCCTGCCTCATCAAATTCGAGGTTTCTTCTCACAGTTTTCTCAGATGACACTTGTTGGCCGCTCATCAGATAGTTGTACAGCTTGCTCCAGCATGGTTGTATCTGAATACCGAAATAGGGGGATGGATTTTATTCTTCAAGCGATTAACCATCCAACTTATTTGGAGGATTTGACCGGACTTACTGAGTTAATGAAGTCAACTAGCTCTTTTCAATTGGACTGGGACAATGACTCAGATGGTAGTGGTGATGATGATGGATGTATTGAAATTTGATACAAACTTTCATCTTGCTTGTATACAAATTACATCAATTCAACAACATTTTTTTGTACATAAATACTGAACAAATGAAATTCCAAATTTGGTATGTCTTCAGTTTTACTTGGTAGGTAAAAATCTGGTTGCTTCCAGATCGCTGAATACATAACTTTCTCTGGTATTTTGTTTATTATCGTTTTAATGCTCGGTGATGTGCATAGATAATATATCAAAGATGAAATTAATATTTTAACATATTACATTAATAAACAGAACAACACAACCCAACGAAATGTCCACTTTTTACGATACCTTTTAGGTGCCTCTGCCCATAAGGCCCAATGGAAATCGATTTGTTTGTCCTTGGAATTGATAAAAACGCAACTTGTTCTCAACTAGCACCAGTGGTCTAGTGGTAGAATAGTACCCTGCCAC

Coding sequence (CDS)

ATGGCCTACGATTACGGATCTATATTGCAATTCGCACCACTTCAAAGCGCTGTCGACGAAGGGTTCTGGCATAGACTCTCTTCCTTGAAGCTCAACCAGCTAGGCATCGACGATTCCCCTATCCCCATCACTGGTTTCTTTGCTCCTTGTTCACATTCCCTACTGTCCAATCATTTGACACTTCTATCTGAGTCATTGCCTATCGAAGTAAGGAGGGACTCATCGACACCATTAACTACTAAAGGCAATAGGAATAGGTGCGCTGTTCCTGGAATTTTATACAATACTAATACTGTAGAGAGCTTCCATGCTCTTGACAGGCTTAGCTTACTAAAGTCTGAAGCAAATAAGATTTGGGAGGATATTCGCAGCGGCAAAGCTTTAGAGGATAGTTCAGTACTTGCAAGGTTCCTTTTAATCTCATTCGCAGACTTGAAAATATGGAACTTTCACTATTGCTTTGCTTTCCCTGCTCTTGTTCTTGATCCTCCAGCAACTGTAGTTGGACTGACATCTGCTTCACAGTGGTTTAACTTCAAAGAGGCTGAATCATTGTTCACAGCTTTTGGTCAGTGGCGCAGCTCAGATCTAACTTCTGACATACCTTTTTTCTTGGTGAATATTGATTCCAGTTCTCAAGCTAGCATTAAGCATCTGAGAGATTTTGAAACCTGCCAAAACAATGGGGGAAAGCTACTTTTTGGCTTTTATGACCCTTGTCATCTTCCAAGTAATCCCGGCTGGCCTCTTCGCAACTTCTTAGCTCTGATTTATTCGAAGTGGAATCTGAAGTCAGTTGACTTTTTATGCTATAGAGAGAACCGTGGTTTTGCTGATCTTAGGTTATCCCTTGTTGGTACAGCCCTGATTGATGATCCCAAAGGGTTCAGAGATCCATCCTGTATGCCTAATCCGGTTGGATGGGAACTGAAAAGAGGGAAGAAATTCTACAAAACTATCAACCTTGCAAAATCCATGGACCCAACTCGGTTAGCTATATCAGCAGCAGATTTGAATTTGAGACTAATGAGGTGGCGTGCTTTGCCTTCTTTAAACATAAACATGTTGTCTTCTCTCAAGTGTCTCTTGCTAGGGGCAGGTACTCTTGGCTGTCAGGTTGCTCGTATGCTTATGGCATGGGGTGTACGAAAAATTACACTAGTGGACAGTGGAAGGGTAGCTATGTCTAATCCATTGAGGCAATCACTTTACACATTGGAGGACTGCCTTAATGGTGGTAACTTTAAAGCTGAAGCAGCAGTTAAAAGTCTCAATCGAATTTTTCCCGCCATGGAAGCTGAAGGTGTTGTGATTTCCATACCAATGCCTGGGCATCCTGTTCCAGACCATGAAGCAGCCAGCACAATTGATGATTGCAGACGACTTGATGACTTAATTAATTCTCATGATGCAATTTTCTTGTTGACTGATACTAGGGAAAGTAGGTGGCTTCCTACACTTCTATGTGCCAATGCAAACAAGGTTACTATCACCGCAGCATTGGGGTTTGACAGTTTCTTAGTTATGCGCCATGGAGCTGGTCCTTCTGGTTCCTGGCATGACTCCACATCTCAAACTAATATAGCTAACCTTTCCTTAAATACTACAAACACGAGGCAGAGATTGGGCTGTTACTTCTGTAATGATGTTGTTGCGCCTATTGATTCAACTGCCAATCGGACATTGGACCAGCAGTGCACTGTTACTCGCCCAGGGCTTGCTCCCATTGCATCAGCGATTGCAGTTGAACTTCTTGTTGGAATTTTACATCATCCTGAAGGAATATATGCTGAAGGTGAACTTTTGAACTCTGGGATTGCTGGAGCTTCTGAGCAGCCTCTTGGAATCCTGCCTCATCAAATTCGAGGTTTCTTCTCACAGTTTTCTCAGATGACACTTGTTGGCCGCTCATCAGATAGTTGTACAGCTTGCTCCAGCATGGTTGTATCTGAATACCGAAATAGGGGGATGGATTTTATTCTTCAAGCGATTAACCATCCAACTTATTTGGAGGATTTGACCGGACTTACTGAGTTAATGAAGTCAACTAGCTCTTTTCAATTGGACTGGGACAATGACTCAGATGGTAGTGGTGATGATGATGGATGTATTGAAATTTGA

Protein sequence

MAYDYGSILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSLLSNHLTLLSESLPIEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKIWEDIRSGKALEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVVGLTSASQWFNFKEAESLFTAFGQWRSSDLTSDIPFFLVNIDSSSQASIKHLRDFETCQNNGGKLLFGFYDPCHLPSNPGWPLRNFLALIYSKWNLKSVDFLCYRENRGFADLRLSLVGTALIDDPKGFRDPSCMPNPVGWELKRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSSLKCLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAEAAVKSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAIFLLTDTRESRWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSWHDSTSQTNIANLSLNTTNTRQRLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGELLNSGIAGASEQPLGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMDFILQAINHPTYLEDLTGLTELMKSTSSFQLDWDNDSDGSGDDDGCIEI*
Homology
BLAST of CsGy6G034860 vs. ExPASy Swiss-Prot
Match: Q94CD5 (Ubiquitin-like modifier-activating enzyme atg7 OS=Arabidopsis thaliana OX=3702 GN=ATG7 PE=1 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 2.4e-279
Identity = 477/702 (67.95%), Postives = 564/702 (80.34%), Query Frame = 0

Query: 8   ILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSLLSNHLTLLSESLP 67
           ILQFAPL S+VDEGFWH  SSLKL++LGIDDSPI ITGF+ PC H  +SNHLTLLSESLP
Sbjct: 10  ILQFAPLNSSVDEGFWHSFSSLKLDKLGIDDSPISITGFYGPCGHPQVSNHLTLLSESLP 69

Query: 68  IEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKIWEDIRSGKA 127
           ++    S    T+ GNRN+C VPGILYNTNTVESF+ LD+ SLLK+EANKIWEDI+SGKA
Sbjct: 70  LD--EQSLIASTSHGNRNKCPVPGILYNTNTVESFNKLDKQSLLKAEANKIWEDIQSGKA 129

Query: 128 LEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVVGLTSASQWFNFKEAESLFT 187
           LED SVL RFL+ISFADLK W+F Y FAFPA VLDPP +++ L  AS++F+ +EAES+  
Sbjct: 130 LEDPSVLPRFLVISFADLKKWSFRYWFAFPAFVLDPPVSLIELKPASEYFSSEEAESVSA 189

Query: 188 AFGQWRSSDLTSDIPFFLVNIDSSSQASIKHLRDFETCQNNGGKLLFGFYDPCHLPSNPG 247
           A   WR SDLT+D+PFFLV++ S S+ASI+HL+D E CQ +  KLLFGFYDPCHLPSNPG
Sbjct: 190 ACNDWRDSDLTTDVPFFLVSVSSDSKASIRHLKDLEACQGDHQKLLFGFYDPCHLPSNPG 249

Query: 248 WPLRNFLALIYSKWNLKSVDFLCYRENRGFADLRLSLVGTALIDDPKGFRDPSCMPNPVG 307
           WPLRN+LALI S+WNL++V F CYRE+RGFADL LSLVG A I    G      +PN VG
Sbjct: 250 WPLRNYLALIRSRWNLETVWFFCYRESRGFADLNLSLVGQASITLSSG-ESAETVPNSVG 309

Query: 308 WELKRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSSLKCLLLGAG 367
           WEL +GK+  ++I+LA SMDPTRLA+SA DLNL+LMRWRALPSLN+N+LSS+KCLLLGAG
Sbjct: 310 WELNKGKRVPRSISLANSMDPTRLAVSAVDLNLKLMRWRALPSLNLNVLSSVKCLLLGAG 369

Query: 368 TLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAEAAVKSLNRI 427
           TLGCQVAR LM WG+R IT VD G+VAMSNP+RQSLY  EDCL  G FKA AAVKSL +I
Sbjct: 370 TLGCQVARTLMGWGIRNITFVDYGKVAMSNPVRQSLYNFEDCLGRGEFKAVAAVKSLKQI 429

Query: 428 FPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAIFLLTDTRESRWLPTL 487
           FPAME  GVV++IPMPGHP+   E  S + DC+RL +LI SHDA+FLLTDTRESRWLP+L
Sbjct: 430 FPAMETSGVVMAIPMPGHPISSQEEDSVLGDCKRLSELIESHDAVFLLTDTRESRWLPSL 489

Query: 488 LCANANKVTITAALGFDSFLVMRHGAGPSGSWHDSTSQTNIANLSLNTTNTRQRLGCYFC 547
           LCANANK+ I AALGFDS++VMRHGAGP+    D      + NL +N TNT QRLGCYFC
Sbjct: 490 LCANANKIAINAALGFDSYMVMRHGAGPTSLSDD------MQNLDINKTNT-QRLGCYFC 549

Query: 548 NDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGE---LLNS 607
           NDVVAP DS  +RTLDQQCTVTRPGLAPIA A+AVELLVG+L HP GI A+G+   L N+
Sbjct: 550 NDVVAPQDSMTDRTLDQQCTVTRPGLAPIAGALAVELLVGVLQHPLGINAKGDNSSLSNT 609

Query: 608 GIAGASEQPLGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMDFILQAI 667
           G     + PLGILPHQIRG  SQFSQ+TL+G++S+SCTACS  V+SEYR RG  FIL+AI
Sbjct: 610 G--NNDDSPLGILPHQIRGSVSQFSQITLLGQASNSCTACSETVISEYRERGNSFILEAI 669

Query: 668 NHPTYLEDLTGLTELMKSTSSFQLDWDNDSDGSGDDDGCIEI 707
           NHPTYLEDLTGLTEL K+ +SF LDW++D   + DDD  +++
Sbjct: 670 NHPTYLEDLTGLTELKKAANSFNLDWEDDD--TDDDDVAVDL 697

BLAST of CsGy6G034860 vs. ExPASy Swiss-Prot
Match: O95352 (Ubiquitin-like modifier-activating enzyme ATG7 OS=Homo sapiens OX=9606 GN=ATG7 PE=1 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 3.3e-159
Identity = 322/712 (45.22%), Postives = 440/712 (61.80%), Query Frame = 0

Query: 7   SILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSLLSNHLTLLSESL 66
           S LQFAP  SA+D GFWH L+  KLN+  +D++P  I G++     + L   LTL   + 
Sbjct: 11  SKLQFAPFSSALDVGFWHELTQKKLNEYRLDEAPKDIKGYYYNGDSAGLPARLTLEFSAF 70

Query: 67  PIEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKIWEDIRSGK 126
                 D S P   +     C   G LYNTNT+ESF   D+  LL+  AN+IWE I+SG 
Sbjct: 71  ------DMSAPTPAR----CCPAIGTLYNTNTLESFKTADKKLLLEQAANEIWESIKSGT 130

Query: 127 ALEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVV-GLTSASQWFNFKEAESL 186
           ALE+  +L +FLL++FADLK ++F+Y F +PAL L     ++ G     Q F+ K+ E+L
Sbjct: 131 ALENPVLLNKFLLLTFADLKKYHFYYWFCYPALCLPESLPLIQGPVGLDQRFSLKQIEAL 190

Query: 187 FTAFGQWRSSDLTSDIPFFLVNIDSSS--QASIKHLRDFETCQNNGGKLLFGFYDPCHLP 246
             A+     ++  + +P+FL+  D +    + +KH  DF   Q    K+  G YDPC+L 
Sbjct: 191 ECAYDNLCQTEGVTALPYFLIKYDENMVLVSLLKHYSDF--FQGQRTKITIGVYDPCNLA 250

Query: 247 SNPGWPLRNFLALIYSKW--NLKSVDFLCYREN--RGFADLRLSLVGTALIDDPKGFRDP 306
             PGWPLRNFL L   +W  + +SV+ +C+R+   +G  D+  S++    +  P+    P
Sbjct: 251 QYPGWPLRNFLVLAAHRWSSSFQSVEVVCFRDRTMQGARDVAHSIIFEVKL--PEMAFSP 310

Query: 307 SCMPNPVGWEL-KRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSS 366
            C P  VGWE  ++G    + +NL++ MDP RLA S+ DLNL+LM WR +P+L+++ + S
Sbjct: 311 DC-PKAVGWEKNQKGGMGPRMVNLSECMDPKRLAESSVDLNLKLMCWRLVPTLDLDKVVS 370

Query: 367 LKCLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAE 426
           +KCLLLGAGTLGC VAR LM WGVR IT VD+ +++ SNP+RQ LY  EDCL GG  KA 
Sbjct: 371 VKCLLLGAGTLGCNVARTLMGWGVRHITFVDNAKISYSNPVRQPLYEFEDCLGGGKPKAL 430

Query: 427 AAVKSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRR----LDDLINSHDAIFL 486
           AA   L +IFP + A G  +SIPMPGHPV  + ++ T++  RR    L+ LI SHD +FL
Sbjct: 431 AAADRLQKIFPGVNARGFNMSIPMPGHPV--NFSSVTLEQARRDVEQLEQLIESHDVVFL 490

Query: 487 LTDTRESRWLPTLLCANANKVTITAALGFDSFLVMRH--------GAGPSGSWHDSTSQT 546
           L DTRESRWLP ++ A+  K+ I AALGFD+F+VMRH        GAG     H   S  
Sbjct: 491 LMDTRESRWLPAVIAASKRKLVINAALGFDTFVVMRHGLKKPKQQGAGDLCPNHPVAS-A 550

Query: 547 NIANLSLNTTNTRQRLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLV 606
           ++   SL       +LGCYFCNDVVAP DST +RTLDQQCTV+RPGLA IA A+AVEL+V
Sbjct: 551 DLLGSSLFANIPGYKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAVIAGALAVELMV 610

Query: 607 GILHHPEGIYAEGELLNSGIAGASEQP--LGILPHQIRGFFSQFSQMTLVGRSSDSCTAC 666
            +L HPEG YA   + +S     +E P  LG++PHQIRGF S+F  +  V  + D CTAC
Sbjct: 611 SVLQHPEGGYA---IASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFDKCTAC 670

Query: 667 SSMVVSEYRNRGMDFILQAINHP-TYLEDLTGLTELMKSTSSFQLDWDNDSD 696
           SS V+ +Y   G +F+ +  N   ++LEDLTGLT L + T + ++ WD   D
Sbjct: 671 SSKVLDQYEREGFNFLAKVFNSSHSFLEDLTGLTLLHQETQAAEI-WDMSDD 700

BLAST of CsGy6G034860 vs. ExPASy Swiss-Prot
Match: Q5ZKY2 (Ubiquitin-like modifier-activating enzyme ATG7 OS=Gallus gallus OX=9031 GN=ATG7 PE=2 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 5.6e-159
Identity = 318/708 (44.92%), Postives = 440/708 (62.15%), Query Frame = 0

Query: 7   SILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSLLSNHLTLLSESL 66
           S LQFAP  SA++ GFWH L+  KLN+  +D++P  I G++     S     LTL   + 
Sbjct: 17  SKLQFAPFSSALNVGFWHELTQKKLNEYRLDETPKVIKGYYYNGDPSGFPARLTLEYSAF 76

Query: 67  PIEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKIWEDIRSGK 126
            I    ++S P         C   G LYNTNT E+F + D+ SLL+ EAN+IWE I+SG 
Sbjct: 77  DI----NASIPARC------CPAFGTLYNTNTFETFKSCDKKSLLEKEANEIWESIKSGA 136

Query: 127 ALEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVVGL-TSASQWFNFKEAESL 186
           ALE+  +L RFLL++FADLK ++F+Y F +PAL       V+         F+  + ++L
Sbjct: 137 ALENPMLLNRFLLLTFADLKKYHFYYWFCYPALCFPDGIHVIQKPVCLGDRFSLNQIQAL 196

Query: 187 FTAFGQWRSSDLTSDIPFFLVNIDSSSQASIKHLRDFE-TCQNNGGKLLFGFYDPCHLPS 246
             A+ +   ++  +  P+FL+    +S   +  L+ ++   Q+ GGK+  G YDPC+L  
Sbjct: 197 QKAYDELCQTEGVTAFPYFLIKYHDNS-VVVSPLKKWDGFFQDQGGKVTVGVYDPCNLSH 256

Query: 247 NPGWPLRNFLALIYSKWN--LKSVDFLCYREN--RGFADLRLSLVGTALIDDPKGFRDPS 306
            PGWPLRNFL L   KW   L+S++ LC+R+   +G  D+  S++    I  P+G   P 
Sbjct: 257 YPGWPLRNFLILASHKWGNILQSIEVLCFRDRTMQGVRDITHSIIFE--IKLPQGAFGPD 316

Query: 307 CMPNPVGWEL-KRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSSL 366
           C P  VGWE  ++G    + +NL++ MDP RLA S+ DLNL+LM WR +P+L++  + S 
Sbjct: 317 C-PKAVGWEKNQKGGMGPRVVNLSECMDPKRLAESSVDLNLKLMCWRLVPTLDLEKIVSA 376

Query: 367 KCLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAEA 426
           KCLLLGAGTLGC VAR LM WGVRKIT VD+ R++ SNP+RQ LY  EDCL+GG  KA A
Sbjct: 377 KCLLLGAGTLGCSVARTLMGWGVRKITFVDNARISYSNPVRQPLYEFEDCLSGGKPKALA 436

Query: 427 AVKSLNRIFPAMEAEGVVISIPMPGHPVPDHEA--ASTIDDCRRLDDLINSHDAIFLLTD 486
           A + L +IFP + +EG  +SIPMPGHPV   E   A    D   L++LI++HD +FLL D
Sbjct: 437 AAERLQKIFPGVNSEGYNMSIPMPGHPVNFSEVTMAQARKDVATLEELIDAHDVVFLLMD 496

Query: 487 TRESRWLPTLLCANANKVTITAALGFDSFLVMRHGAGP-------SGSWHDSTSQTNIAN 546
           TRESRWLP ++ A+  K+ I AALGFD+F+VMRHG          +  +  +   +++  
Sbjct: 497 TRESRWLPAVIAASKRKLVINAALGFDTFVVMRHGLKKPKQQETGNACFSTAPGPSDLLG 556

Query: 547 LSLNTTNTRQRLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILH 606
            SL +     +LGCYFCNDVVAP DST +RTLDQQCTV+RPGLA IA A+AVEL+V +L 
Sbjct: 557 SSLFSNIPGYKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAMIAGALAVELMVSVLQ 616

Query: 607 HPEGIYAEGELLNSGIAGASEQP--LGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMV 666
           HPEG YA   + +S     +E P  LG++PHQIRGF S+F  +  V  + D CTACS  V
Sbjct: 617 HPEGGYA---VASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFDKCTACSPKV 676

Query: 667 VSEYRNRGMDFILQAINHP-TYLEDLTGLTELMKSTSSFQLDWDNDSD 696
           + +Y   G +F+ +  N   ++LEDLTGLT L + T + ++ WD   D
Sbjct: 677 LDQYEREGFNFLAKVFNSSHSFLEDLTGLTLLHQETQAAEI-WDMSDD 706

BLAST of CsGy6G034860 vs. ExPASy Swiss-Prot
Match: Q641Y5 (Ubiquitin-like modifier-activating enzyme ATG7 OS=Rattus norvegicus OX=10116 GN=Atg7 PE=2 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 7.6e-156
Identity = 316/704 (44.89%), Postives = 432/704 (61.36%), Query Frame = 0

Query: 7   SILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSLLSNHLTLLSESL 66
           S LQFAP  SA+D GFWH L+  KLN+  +D++P  I G++     + L   LTL   + 
Sbjct: 7   SKLQFAPFNSALDVGFWHELTQKKLNEYRLDEAPKDIKGYYYNGDSAGLPTRLTLEFSAF 66

Query: 67  PIEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKIWEDIRSGK 126
                 D S P   +     C   G L+NTNT+E+F   D+  LL+  AN+IWE I+SG 
Sbjct: 67  ------DMSAPTPAR----CCPAMGTLHNTNTLEAFKTADKKLLLEQSANEIWEAIKSGA 126

Query: 127 ALEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVV-GLTSASQWFNFKEAESL 186
           ALE+  +L +FLL++FADLK ++F+Y F  PAL L     ++ G     Q  + K+ ++L
Sbjct: 127 ALENPMLLNKFLLLTFADLKKYHFYYWFCCPALCLPESIPLIRGPVGLDQRLSPKQIQAL 186

Query: 187 FTAFGQWRSSDLTSDIPFFLVNIDSSS--QASIKHLRDFETCQNNGGKLLFGFYDPCHLP 246
             A+     ++  + +P+FL   D  +   + +KH  DF   Q    KL  G YDPC+L 
Sbjct: 187 EHAYDDLCRTEGVTALPYFLFKYDDDTVLVSLLKHYSDF--FQGQRTKLTVGVYDPCNLT 246

Query: 247 SNPGWPLRNFLALIYSKW--NLKSVDFLCYREN--RGFADLRLSLVGTALIDDPKGFRDP 306
            +PGWPLRNFL L   +W  + +SV+ LC+R+   +G  D+  S++    +  P+    P
Sbjct: 247 QHPGWPLRNFLVLAAHRWSGSFQSVEVLCFRDRTMQGARDVTHSIIFEVKL--PEMAFSP 306

Query: 307 SCMPNPVGWEL-KRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSS 366
            C P  VGWE  ++G    + +NL+  MDP RLA S+ DLNL+LM WR +P+L+++ + S
Sbjct: 307 DC-PKAVGWEKNQKGGMGPRMVNLSGCMDPKRLAESSVDLNLKLMCWRLVPTLDLDKVVS 366

Query: 367 LKCLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAE 426
           +KCLLLGAGTLGC VAR LM WGVR +T VD+ +++ SNP+RQ LY  EDCL GG  KA 
Sbjct: 367 VKCLLLGAGTLGCNVARTLMGWGVRHVTFVDNAKISYSNPVRQPLYEFEDCLGGGKPKAL 426

Query: 427 AAVKSLNRIFPAMEAEGVVISIPMPGHPV--PDHEAASTIDDCRRLDDLINSHDAIFLLT 486
           AA + L +IFP + A G  +SIPMPGHPV   D        D  +L++LI+SHD IFLL 
Sbjct: 427 AAAERLQKIFPGVNASGFNMSIPMPGHPVNFSDVTMEQARRDVEQLEELIDSHDVIFLLM 486

Query: 487 DTRESRWLPTLLCANANKVTITAALGFDSFLVMRHG-AGPSGSWHDSTSQTNIA-----N 546
           DTRESRWLPT++ A+  K+ I AALGFD+F+VMRHG   P          +++       
Sbjct: 487 DTRESRWLPTVIAASKRKLVINAALGFDTFVVMRHGLKKPKQQGAGDLCPSHLVAPADLG 546

Query: 547 LSLNTTNTRQRLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILH 606
            SL       +LGCYFCNDVVAP DST +RTLDQQCTV+RPGLA IA A+AVEL+V +L 
Sbjct: 547 SSLFANIPGYKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAVIAGALAVELMVSVLQ 606

Query: 607 HPEGIYAEGELLNSGIAGASEQP--LGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMV 666
           HPEG YA   + +S     +E P  LG++PHQIRGF S+F  +  V  + D CTACSS V
Sbjct: 607 HPEGGYA---IASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFDKCTACSSKV 666

Query: 667 VSEYRNRGMDFILQAINHP-TYLEDLTGLTELMKSTSSFQLDWD 692
           + +Y   G  F+ +  N   ++LEDLTGLT L + T + ++ WD
Sbjct: 667 LDQYEQEGFTFLAKVFNSSHSFLEDLTGLTLLHQETQAAEI-WD 691

BLAST of CsGy6G034860 vs. ExPASy Swiss-Prot
Match: Q9D906 (Ubiquitin-like modifier-activating enzyme ATG7 OS=Mus musculus OX=10090 GN=Atg7 PE=1 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 1.4e-154
Identity = 313/702 (44.59%), Postives = 431/702 (61.40%), Query Frame = 0

Query: 9   LQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSLLSNHLTLLSESLPI 68
           LQFAP  SA+D GFWH L+  KLN+  +D++P  I G++     + L   LTL   +  +
Sbjct: 9   LQFAPFNSALDVGFWHELTQKKLNEYRLDEAPKDIKGYYYNGDSAGLPTRLTLEFSAFDM 68

Query: 69  EVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKIWEDIRSGKAL 128
                +STP       + C   G L+NTNT+E+F   D+  LL+  AN+IWE I+SG AL
Sbjct: 69  ----SASTP------AHCCPAMGTLHNTNTLEAFKTADKKLLLEQSANEIWEAIKSGAAL 128

Query: 129 EDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVV-GLTSASQWFNFKEAESLFT 188
           E+  +L +FLL++FADLK ++F+Y F  PAL L     ++ G  S  Q  + K+ ++L  
Sbjct: 129 ENPMLLNKFLLLTFADLKKYHFYYWFCCPALCLPESIPLIRGPVSLDQRLSPKQIQALEH 188

Query: 189 AFGQWRSSDLTSDIPFFLVNIDSSS--QASIKHLRDFETCQNNGGKLLFGFYDPCHLPSN 248
           A+     ++  + +P+FL   D  +   + +KH  DF   Q    K+  G YDPC+L   
Sbjct: 189 AYDDLCRAEGVTALPYFLFKYDDDTVLVSLLKHYSDF--FQGQRTKITVGVYDPCNLAQY 248

Query: 249 PGWPLRNFLALIYSKW--NLKSVDFLCYREN--RGFADLRLSLVGTALIDDPKGFRDPSC 308
           PGWPLRNFL L   +W  + +SV+ LC+R+   +G  D+  S++    +  P+    P C
Sbjct: 249 PGWPLRNFLVLAAHRWSGSFQSVEVLCFRDRTMQGARDVTHSIIFEVKL--PEMAFSPDC 308

Query: 309 MPNPVGWEL-KRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSSLK 368
            P  VGWE  ++G    + +NL+  MDP RLA S+ DLNL+LM WR +P+L+++ + S+K
Sbjct: 309 -PKAVGWEKNQKGGMGPRMVNLSGCMDPKRLAESSVDLNLKLMCWRLVPTLDLDKVVSVK 368

Query: 369 CLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAEAA 428
           CLLLGAGTLGC VAR LM WGVR +T VD+ +++ SNP+RQ LY  EDCL GG  KA AA
Sbjct: 369 CLLLGAGTLGCNVARTLMGWGVRHVTFVDNAKISYSNPVRQPLYEFEDCLGGGKPKALAA 428

Query: 429 VKSLNRIFPAMEAEGVVISIPMPGHPV--PDHEAASTIDDCRRLDDLINSHDAIFLLTDT 488
            + L +IFP + A G  +SIPMPGHPV   D        D  +L+ LI++HD IFLL DT
Sbjct: 429 AERLQKIFPGVNARGFNMSIPMPGHPVNFSDVTMEQARRDVEQLEQLIDNHDVIFLLMDT 488

Query: 489 RESRWLPTLLCANANKVTITAALGFDSFLVMRHG-AGPSGSWHDSTSQTNIA-----NLS 548
           RESRWLPT++ A+  K+ I AALGFD+F+VMRHG   P          +++        S
Sbjct: 489 RESRWLPTVIAASKRKLVINAALGFDTFVVMRHGLKKPKQQGAGDLCPSHLVAPADLGSS 548

Query: 549 LNTTNTRQRLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHP 608
           L       +LGCYFCNDVVAP DST +RTLDQQCTV+RPGLA IA A+AVEL+V +L HP
Sbjct: 549 LFANIPGYKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAVIAGALAVELMVSVLQHP 608

Query: 609 EGIYAEGELLNSGIAGASEQP--LGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMVVS 668
           EG YA   + +S     +E P  LG++PHQIRGF S+F  +  V  + D CTACS  V+ 
Sbjct: 609 EGGYA---IASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFDKCTACSPKVLD 668

Query: 669 EYRNRGMDFILQAINHP-TYLEDLTGLTELMKSTSSFQLDWD 692
           +Y   G  F+ +  N   ++LEDLTGLT L + T + ++ WD
Sbjct: 669 QYEREGFTFLAKVFNSSHSFLEDLTGLTLLHQETQAAEI-WD 691

BLAST of CsGy6G034860 vs. NCBI nr
Match: XP_011658247.1 (ubiquitin-like modifier-activating enzyme atg7 [Cucumis sativus] >KGN49301.1 hypothetical protein Csa_002926 [Cucumis sativus])

HSP 1 Score: 1428 bits (3696), Expect = 0.0
Identity = 706/706 (100.00%), Postives = 706/706 (100.00%), Query Frame = 0

Query: 1   MAYDYGSILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSLLSNHLT 60
           MAYDYGSILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSLLSNHLT
Sbjct: 1   MAYDYGSILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSLLSNHLT 60

Query: 61  LLSESLPIEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKIWE 120
           LLSESLPIEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKIWE
Sbjct: 61  LLSESLPIEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKIWE 120

Query: 121 DIRSGKALEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVVGLTSASQWFNFK 180
           DIRSGKALEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVVGLTSASQWFNFK
Sbjct: 121 DIRSGKALEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVVGLTSASQWFNFK 180

Query: 181 EAESLFTAFGQWRSSDLTSDIPFFLVNIDSSSQASIKHLRDFETCQNNGGKLLFGFYDPC 240
           EAESLFTAFGQWRSSDLTSDIPFFLVNIDSSSQASIKHLRDFETCQNNGGKLLFGFYDPC
Sbjct: 181 EAESLFTAFGQWRSSDLTSDIPFFLVNIDSSSQASIKHLRDFETCQNNGGKLLFGFYDPC 240

Query: 241 HLPSNPGWPLRNFLALIYSKWNLKSVDFLCYRENRGFADLRLSLVGTALIDDPKGFRDPS 300
           HLPSNPGWPLRNFLALIYSKWNLKSVDFLCYRENRGFADLRLSLVGTALIDDPKGFRDPS
Sbjct: 241 HLPSNPGWPLRNFLALIYSKWNLKSVDFLCYRENRGFADLRLSLVGTALIDDPKGFRDPS 300

Query: 301 CMPNPVGWELKRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSSLK 360
           CMPNPVGWELKRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSSLK
Sbjct: 301 CMPNPVGWELKRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSSLK 360

Query: 361 CLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAEAA 420
           CLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAEAA
Sbjct: 361 CLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAEAA 420

Query: 421 VKSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAIFLLTDTRE 480
           VKSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAIFLLTDTRE
Sbjct: 421 VKSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAIFLLTDTRE 480

Query: 481 SRWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSWHDSTSQTNIANLSLNTTNTRQ 540
           SRWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSWHDSTSQTNIANLSLNTTNTRQ
Sbjct: 481 SRWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSWHDSTSQTNIANLSLNTTNTRQ 540

Query: 541 RLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGE 600
           RLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGE
Sbjct: 541 RLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGE 600

Query: 601 LLNSGIAGASEQPLGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMDFI 660
           LLNSGIAGASEQPLGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMDFI
Sbjct: 601 LLNSGIAGASEQPLGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMDFI 660

Query: 661 LQAINHPTYLEDLTGLTELMKSTSSFQLDWDNDSDGSGDDDGCIEI 706
           LQAINHPTYLEDLTGLTELMKSTSSFQLDWDNDSDGSGDDDGCIEI
Sbjct: 661 LQAINHPTYLEDLTGLTELMKSTSSFQLDWDNDSDGSGDDDGCIEI 706

BLAST of CsGy6G034860 vs. NCBI nr
Match: XP_008439746.1 (PREDICTED: ubiquitin-like modifier-activating enzyme atg7 [Cucumis melo])

HSP 1 Score: 1337 bits (3459), Expect = 0.0
Identity = 665/705 (94.33%), Postives = 679/705 (96.31%), Query Frame = 0

Query: 2   AYDYGSILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSLLSNHLTL 61
           A D GSILQFAP QSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHS +SNHLTL
Sbjct: 3   ANDKGSILQFAPFQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSQVSNHLTL 62

Query: 62  LSESLPIEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKIWED 121
           LSESLP EV+RDSSTPLTTKGNRNRCAVPGILYNTNTVESFHAL++LSLLKSEA KIWED
Sbjct: 63  LSESLPTEVKRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALEKLSLLKSEAKKIWED 122

Query: 122 IRSGKALEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVVGLTSASQWFNFKE 181
           I SGKALEDSSVLARFLLISFADLK WNFHY FAFPALVLDPPATVV L SASQWFN +E
Sbjct: 123 ILSGKALEDSSVLARFLLISFADLKKWNFHYWFAFPALVLDPPATVVELKSASQWFNLEE 182

Query: 182 AESLFTAFGQWRSSDLTSDIPFFLVNIDSSSQASIKHLRDFETCQNNGGKLLFGFYDPCH 241
           AESL TAFGQWRSSDLTSDIPFFLV IDS+SQASIKHLRDFETCQNNG KLLFGFYDPCH
Sbjct: 183 AESLSTAFGQWRSSDLTSDIPFFLVIIDSNSQASIKHLRDFETCQNNGEKLLFGFYDPCH 242

Query: 242 LPSNPGWPLRNFLALIYSKWNLKSVDFLCYRENRGFADLRLSLVGTALIDDPKGFRDPSC 301
           LPSNPGWPLRNFLALIYS+WNLKSV+FLCYRENRGFADL LSLVG ALIDDPKG RD  C
Sbjct: 243 LPSNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDDPKGLRDQCC 302

Query: 302 MPNPVGWELKRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSSLKC 361
           MPN VGWEL +GKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNIN+LSSLKC
Sbjct: 303 MPNAVGWELNKGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINILSSLKC 362

Query: 362 LLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAEAAV 421
           LLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGG+FKAEAAV
Sbjct: 363 LLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAEAAV 422

Query: 422 KSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAIFLLTDTRES 481
           KSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDA+FLLTDTRES
Sbjct: 423 KSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAVFLLTDTRES 482

Query: 482 RWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSWHDSTSQTNIANLSLNTTNTRQR 541
           RWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGS HDSTSQTNIANLSLN TNTRQR
Sbjct: 483 RWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSCHDSTSQTNIANLSLNATNTRQR 542

Query: 542 LGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGEL 601
           LGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGEL
Sbjct: 543 LGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGEL 602

Query: 602 LNSGIAGASEQPLGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMDFIL 661
           LNSGI GASEQPLGILPHQIRGF SQFSQMTLVGRSS+SCTACSSMVVSEYRNRGMDFIL
Sbjct: 603 LNSGIDGASEQPLGILPHQIRGFLSQFSQMTLVGRSSNSCTACSSMVVSEYRNRGMDFIL 662

Query: 662 QAINHPTYLEDLTGLTELMKSTSSFQLDWDNDSDGSGDDDGCIEI 706
           QAINHPTYLEDLTGLTELMKSTSSFQLDWDND+DGSGDDDGCIEI
Sbjct: 663 QAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSGDDDGCIEI 707

BLAST of CsGy6G034860 vs. NCBI nr
Match: XP_038880974.1 (ubiquitin-like modifier-activating enzyme atg7 [Benincasa hispida])

HSP 1 Score: 1294 bits (3349), Expect = 0.0
Identity = 642/701 (91.58%), Postives = 661/701 (94.29%), Query Frame = 0

Query: 6   GSILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSLLSNHLTLLSES 65
           GSILQFAP QSAVDEGFWHRLSSLKLN+LGIDDSPIPITGFFAPCSHS +SNHL LLSES
Sbjct: 7   GSILQFAPFQSAVDEGFWHRLSSLKLNELGIDDSPIPITGFFAPCSHSQVSNHLILLSES 66

Query: 66  LPIEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKIWEDIRSG 125
           LP +V R+SS P TTKGNRNRC+VPG LYNTNTVESFHALD+LSLLKSEA KIWEDI SG
Sbjct: 67  LPAKVTRESSAPSTTKGNRNRCSVPGTLYNTNTVESFHALDKLSLLKSEAKKIWEDILSG 126

Query: 126 KALEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVVGLTSASQWFNFKEAESL 185
           KALEDSSVLARFLLISFADLK WNFHY FAFPALVL PPATVV L  AS WFN +EAESL
Sbjct: 127 KALEDSSVLARFLLISFADLKKWNFHYWFAFPALVLHPPATVVELKPASHWFNLEEAESL 186

Query: 186 FTAFGQWRSSDLTSDIPFFLVNIDSSSQASIKHLRDFETCQNNGGKLLFGFYDPCHLPSN 245
            TAFGQWRSSDLT DIPFFLVNIDSSSQA IKHLRDFETCQN G KLLFGFYDPCHLP+N
Sbjct: 187 STAFGQWRSSDLTCDIPFFLVNIDSSSQAIIKHLRDFETCQNTGEKLLFGFYDPCHLPNN 246

Query: 246 PGWPLRNFLALIYSKWNLKSVDFLCYRENRGFADLRLSLVGTALIDDPKGFRDPSCMPNP 305
           PGWPLRNFLALIYS+WNLKSV+FLCYRENRGF DL LSLVG ALIDDPKG RD  CMPN 
Sbjct: 247 PGWPLRNFLALIYSRWNLKSVNFLCYRENRGFPDLTLSLVGRALIDDPKGLRDQCCMPNA 306

Query: 306 VGWELKRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSSLKCLLLG 365
           VGWEL +GKK Y+TINLAKSMDPTRLAISAADLNLRLMRWRALPSLNIN+LSSLKCLLLG
Sbjct: 307 VGWELNKGKKCYRTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINVLSSLKCLLLG 366

Query: 366 AGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAEAAVKSLN 425
           AGTLGCQVARMLMAWG+RKITLVDSGRVAMSNP+RQSLYTLEDCLNGG+FKAEAAVKSLN
Sbjct: 367 AGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPMRQSLYTLEDCLNGGDFKAEAAVKSLN 426

Query: 426 RIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAIFLLTDTRESRWLP 485
           RIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDA+FLLTDTRESRWLP
Sbjct: 427 RIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAVFLLTDTRESRWLP 486

Query: 486 TLLCANANKVTITAALGFDSFLVMRHGAGPSGSWHDSTSQTNIANLSLNTTNTRQRLGCY 545
           TLLCAN NKVTITAALGFDSFLVMRHGAGPSGS HDS SQTNIANLSL+ TNTRQRLGCY
Sbjct: 487 TLLCANTNKVTITAALGFDSFLVMRHGAGPSGSCHDSISQTNIANLSLSATNTRQRLGCY 546

Query: 546 FCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGELLNSG 605
           FCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGELLNSG
Sbjct: 547 FCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGELLNSG 606

Query: 606 IAGASEQPLGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMDFILQAIN 665
           I GASEQPLGILPHQIRG  SQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMDFILQAIN
Sbjct: 607 INGASEQPLGILPHQIRGSLSQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMDFILQAIN 666

Query: 666 HPTYLEDLTGLTELMKSTSSFQLDWDNDSDGSGDDDGCIEI 706
           HPTYLEDLTGLTELMKSTSSFQLDWDND+DGSGDDD CIEI
Sbjct: 667 HPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSGDDDECIEI 707

BLAST of CsGy6G034860 vs. NCBI nr
Match: KAA0052623.1 (ubiquitin-like modifier-activating enzyme atg7 [Cucumis melo var. makuwa] >TYK13205.1 ubiquitin-like modifier-activating enzyme atg7 [Cucumis melo var. makuwa])

HSP 1 Score: 1294 bits (3348), Expect = 0.0
Identity = 652/708 (92.09%), Postives = 665/708 (93.93%), Query Frame = 0

Query: 2   AYDYGSILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITG---FFAPCSHSLLSNH 61
           A D GSILQFAP QSAVDEGFWHRLSSLKLNQLGIDDSPIPITG   FF P S       
Sbjct: 3   ANDKGSILQFAPFQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGLFLFFFPFSFFY---- 62

Query: 62  LTLLSESLPIEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKI 121
                 SLP EV+RDSSTPLTTKGNRNRCAVPGILYNTNTVESFHAL++LSLLKSEA KI
Sbjct: 63  ------SLPTEVKRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALEKLSLLKSEAKKI 122

Query: 122 WEDIRSGKALEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVVGLTSASQWFN 181
           WEDI SGKALEDSSVLARFLLISFADLK WNFHY FAFPALVLDPPATVV L SASQWFN
Sbjct: 123 WEDILSGKALEDSSVLARFLLISFADLKKWNFHYWFAFPALVLDPPATVVELKSASQWFN 182

Query: 182 FKEAESLFTAFGQWRSSDLTSDIPFFLVNIDSSSQASIKHLRDFETCQNNGGKLLFGFYD 241
            +EAESL TAFGQWRSSDLTSDIPFFLV IDS+SQASIKHLRDFETCQNNG KLLFGFYD
Sbjct: 183 LEEAESLSTAFGQWRSSDLTSDIPFFLVIIDSNSQASIKHLRDFETCQNNGEKLLFGFYD 242

Query: 242 PCHLPSNPGWPLRNFLALIYSKWNLKSVDFLCYRENRGFADLRLSLVGTALIDDPKGFRD 301
           PCHLPSNPGWPLRNFLALIYS+WNLKSV+FLCYRENRGFADL LSLVG ALIDDPKG RD
Sbjct: 243 PCHLPSNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDDPKGLRD 302

Query: 302 PSCMPNPVGWELKRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSS 361
             CMPN VGWEL +GKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNIN+LSS
Sbjct: 303 QCCMPNAVGWELNKGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINILSS 362

Query: 362 LKCLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAE 421
           LKCLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGG+FKAE
Sbjct: 363 LKCLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAE 422

Query: 422 AAVKSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAIFLLTDT 481
           AAVKSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDA+FLLTDT
Sbjct: 423 AAVKSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAVFLLTDT 482

Query: 482 RESRWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSWHDSTSQTNIANLSLNTTNT 541
           RESRWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGS HDSTSQTNIANLSLN TNT
Sbjct: 483 RESRWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSCHDSTSQTNIANLSLNATNT 542

Query: 542 RQRLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAE 601
           RQRLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAE
Sbjct: 543 RQRLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAE 602

Query: 602 GELLNSGIAGASEQPLGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMD 661
           GELLNSGI GASEQPLGILPHQIRGF SQFSQMTLVGRSS+SCTACSSMVVSEYRNRGMD
Sbjct: 603 GELLNSGIDGASEQPLGILPHQIRGFLSQFSQMTLVGRSSNSCTACSSMVVSEYRNRGMD 662

Query: 662 FILQAINHPTYLEDLTGLTELMKSTSSFQLDWDNDSDGSGDDDGCIEI 706
           FILQAINHPTYLEDLTGLTELMKSTSSFQLDWDND+DGSGDDDGCIEI
Sbjct: 663 FILQAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSGDDDGCIEI 700

BLAST of CsGy6G034860 vs. NCBI nr
Match: XP_022926466.1 (ubiquitin-like modifier-activating enzyme atg7 [Cucurbita moschata])

HSP 1 Score: 1262 bits (3266), Expect = 0.0
Identity = 630/706 (89.24%), Postives = 661/706 (93.63%), Query Frame = 0

Query: 2   AYDYGSILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSLLSNHLTL 61
           A D GSILQFAP QS VDEGFWHRLSSLKLN+LGIDDSPIPITGFFAPC+HS +SNHLTL
Sbjct: 3   ANDKGSILQFAPFQSLVDEGFWHRLSSLKLNELGIDDSPIPITGFFAPCAHSQVSNHLTL 62

Query: 62  LSESLPIEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKIWED 121
           LSESLP EV  +SS PLTTKGNRNRC+VPGILYNTNTVESFHALD+LSLLKSEA KIWED
Sbjct: 63  LSESLPSEVSSESSAPLTTKGNRNRCSVPGILYNTNTVESFHALDKLSLLKSEAKKIWED 122

Query: 122 IRSGKALEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVVGLTSASQWFNFKE 181
           I SGKA+EDSSVLARFLLISFADLK WNFHY FAFPALVL+PPATVV L  AS WFN +E
Sbjct: 123 ILSGKAIEDSSVLARFLLISFADLKKWNFHYWFAFPALVLNPPATVVELKPASHWFNLEE 182

Query: 182 AESLFTAFGQWRSSDLTSDIPFFLVNIDSSSQASIKHLRDFETCQNNGGKLLFGFYDPCH 241
           AES+ ++F QWRSS+LTSDIPFFLVNIDSSSQASIKHLRDFE CQNNG KLLFGFYDPCH
Sbjct: 183 AESVSSSFAQWRSSNLTSDIPFFLVNIDSSSQASIKHLRDFEACQNNGEKLLFGFYDPCH 242

Query: 242 LPSNPGWPLRNFLALIYSKWNLKSVDFLCYRENRGFADLRLSLVGTALIDDPKGFRDPSC 301
           LP+NPGWPLRNFLALIYS+WNLKSV+FLCYRENRGFADL LSLVG ALIDD +GFRD  C
Sbjct: 243 LPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDDTQGFRDQQC 302

Query: 302 MPNPVGWELKRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSSLKC 361
           MPN VGWEL RGKK +K+INLAKSMDPTRLAISAADLNLRLMRWRALPSLNIN+LSSLKC
Sbjct: 303 MPNVVGWELNRGKKCHKSINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINILSSLKC 362

Query: 362 LLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAEAAV 421
           LLLGAGTLGCQVARMLMAWG+RKITLVDSGRVAMSNPLRQSLYT+EDCLNGG+FKAEAAV
Sbjct: 363 LLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTMEDCLNGGDFKAEAAV 422

Query: 422 KSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAIFLLTDTRES 481
           KSLNRIFPAMEAEGVVISIPMPGHPV D+EAAS +DDCRRLDDLINSHDA+FLLTDTRES
Sbjct: 423 KSLNRIFPAMEAEGVVISIPMPGHPVSDNEAASMLDDCRRLDDLINSHDAVFLLTDTRES 482

Query: 482 RWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSWHDSTSQTNIANLSLNTTNTRQR 541
           RWLPTLLCAN NKVTITAALGFDSFLVMRHGAGPSGS HDSTSQTNIANLSLN  NT+QR
Sbjct: 483 RWLPTLLCANTNKVTITAALGFDSFLVMRHGAGPSGSCHDSTSQTNIANLSLNAANTKQR 542

Query: 542 LGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGEL 601
           LGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAE EL
Sbjct: 543 LGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAECEL 602

Query: 602 LNSGIAGASEQPLGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMDFIL 661
            NSG   ASEQPLGILPHQIRG  SQFSQMTLVGRSS++CTACSS+VVSEYRNRGMDFIL
Sbjct: 603 SNSG---ASEQPLGILPHQIRGSLSQFSQMTLVGRSSNTCTACSSLVVSEYRNRGMDFIL 662

Query: 662 QAINHPTYLEDLTGLTELMKSTSSFQLDWDNDSDGSGDDD-GCIEI 706
           QAINHPTYLEDLTGLTELMKSTSSFQLDWDN +DGSGDDD GCIEI
Sbjct: 663 QAINHPTYLEDLTGLTELMKSTSSFQLDWDNYTDGSGDDDDGCIEI 705

BLAST of CsGy6G034860 vs. ExPASy TrEMBL
Match: A0A0A0KNJ9 (Ubiquitin-like modifier-activating enzyme ATG7 OS=Cucumis sativus OX=3659 GN=Csa_6G519540 PE=3 SV=1)

HSP 1 Score: 1428 bits (3696), Expect = 0.0
Identity = 706/706 (100.00%), Postives = 706/706 (100.00%), Query Frame = 0

Query: 1   MAYDYGSILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSLLSNHLT 60
           MAYDYGSILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSLLSNHLT
Sbjct: 1   MAYDYGSILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSLLSNHLT 60

Query: 61  LLSESLPIEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKIWE 120
           LLSESLPIEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKIWE
Sbjct: 61  LLSESLPIEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKIWE 120

Query: 121 DIRSGKALEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVVGLTSASQWFNFK 180
           DIRSGKALEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVVGLTSASQWFNFK
Sbjct: 121 DIRSGKALEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVVGLTSASQWFNFK 180

Query: 181 EAESLFTAFGQWRSSDLTSDIPFFLVNIDSSSQASIKHLRDFETCQNNGGKLLFGFYDPC 240
           EAESLFTAFGQWRSSDLTSDIPFFLVNIDSSSQASIKHLRDFETCQNNGGKLLFGFYDPC
Sbjct: 181 EAESLFTAFGQWRSSDLTSDIPFFLVNIDSSSQASIKHLRDFETCQNNGGKLLFGFYDPC 240

Query: 241 HLPSNPGWPLRNFLALIYSKWNLKSVDFLCYRENRGFADLRLSLVGTALIDDPKGFRDPS 300
           HLPSNPGWPLRNFLALIYSKWNLKSVDFLCYRENRGFADLRLSLVGTALIDDPKGFRDPS
Sbjct: 241 HLPSNPGWPLRNFLALIYSKWNLKSVDFLCYRENRGFADLRLSLVGTALIDDPKGFRDPS 300

Query: 301 CMPNPVGWELKRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSSLK 360
           CMPNPVGWELKRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSSLK
Sbjct: 301 CMPNPVGWELKRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSSLK 360

Query: 361 CLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAEAA 420
           CLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAEAA
Sbjct: 361 CLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAEAA 420

Query: 421 VKSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAIFLLTDTRE 480
           VKSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAIFLLTDTRE
Sbjct: 421 VKSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAIFLLTDTRE 480

Query: 481 SRWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSWHDSTSQTNIANLSLNTTNTRQ 540
           SRWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSWHDSTSQTNIANLSLNTTNTRQ
Sbjct: 481 SRWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSWHDSTSQTNIANLSLNTTNTRQ 540

Query: 541 RLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGE 600
           RLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGE
Sbjct: 541 RLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGE 600

Query: 601 LLNSGIAGASEQPLGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMDFI 660
           LLNSGIAGASEQPLGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMDFI
Sbjct: 601 LLNSGIAGASEQPLGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMDFI 660

Query: 661 LQAINHPTYLEDLTGLTELMKSTSSFQLDWDNDSDGSGDDDGCIEI 706
           LQAINHPTYLEDLTGLTELMKSTSSFQLDWDNDSDGSGDDDGCIEI
Sbjct: 661 LQAINHPTYLEDLTGLTELMKSTSSFQLDWDNDSDGSGDDDGCIEI 706

BLAST of CsGy6G034860 vs. ExPASy TrEMBL
Match: A0A1S3AZ28 (Ubiquitin-like modifier-activating enzyme ATG7 OS=Cucumis melo OX=3656 GN=LOC103484451 PE=3 SV=1)

HSP 1 Score: 1337 bits (3459), Expect = 0.0
Identity = 665/705 (94.33%), Postives = 679/705 (96.31%), Query Frame = 0

Query: 2   AYDYGSILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSLLSNHLTL 61
           A D GSILQFAP QSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHS +SNHLTL
Sbjct: 3   ANDKGSILQFAPFQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSQVSNHLTL 62

Query: 62  LSESLPIEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKIWED 121
           LSESLP EV+RDSSTPLTTKGNRNRCAVPGILYNTNTVESFHAL++LSLLKSEA KIWED
Sbjct: 63  LSESLPTEVKRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALEKLSLLKSEAKKIWED 122

Query: 122 IRSGKALEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVVGLTSASQWFNFKE 181
           I SGKALEDSSVLARFLLISFADLK WNFHY FAFPALVLDPPATVV L SASQWFN +E
Sbjct: 123 ILSGKALEDSSVLARFLLISFADLKKWNFHYWFAFPALVLDPPATVVELKSASQWFNLEE 182

Query: 182 AESLFTAFGQWRSSDLTSDIPFFLVNIDSSSQASIKHLRDFETCQNNGGKLLFGFYDPCH 241
           AESL TAFGQWRSSDLTSDIPFFLV IDS+SQASIKHLRDFETCQNNG KLLFGFYDPCH
Sbjct: 183 AESLSTAFGQWRSSDLTSDIPFFLVIIDSNSQASIKHLRDFETCQNNGEKLLFGFYDPCH 242

Query: 242 LPSNPGWPLRNFLALIYSKWNLKSVDFLCYRENRGFADLRLSLVGTALIDDPKGFRDPSC 301
           LPSNPGWPLRNFLALIYS+WNLKSV+FLCYRENRGFADL LSLVG ALIDDPKG RD  C
Sbjct: 243 LPSNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDDPKGLRDQCC 302

Query: 302 MPNPVGWELKRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSSLKC 361
           MPN VGWEL +GKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNIN+LSSLKC
Sbjct: 303 MPNAVGWELNKGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINILSSLKC 362

Query: 362 LLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAEAAV 421
           LLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGG+FKAEAAV
Sbjct: 363 LLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAEAAV 422

Query: 422 KSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAIFLLTDTRES 481
           KSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDA+FLLTDTRES
Sbjct: 423 KSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAVFLLTDTRES 482

Query: 482 RWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSWHDSTSQTNIANLSLNTTNTRQR 541
           RWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGS HDSTSQTNIANLSLN TNTRQR
Sbjct: 483 RWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSCHDSTSQTNIANLSLNATNTRQR 542

Query: 542 LGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGEL 601
           LGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGEL
Sbjct: 543 LGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGEL 602

Query: 602 LNSGIAGASEQPLGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMDFIL 661
           LNSGI GASEQPLGILPHQIRGF SQFSQMTLVGRSS+SCTACSSMVVSEYRNRGMDFIL
Sbjct: 603 LNSGIDGASEQPLGILPHQIRGFLSQFSQMTLVGRSSNSCTACSSMVVSEYRNRGMDFIL 662

Query: 662 QAINHPTYLEDLTGLTELMKSTSSFQLDWDNDSDGSGDDDGCIEI 706
           QAINHPTYLEDLTGLTELMKSTSSFQLDWDND+DGSGDDDGCIEI
Sbjct: 663 QAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSGDDDGCIEI 707

BLAST of CsGy6G034860 vs. ExPASy TrEMBL
Match: A0A5D3CNQ1 (Ubiquitin-like modifier-activating enzyme ATG7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008180 PE=3 SV=1)

HSP 1 Score: 1294 bits (3348), Expect = 0.0
Identity = 652/708 (92.09%), Postives = 665/708 (93.93%), Query Frame = 0

Query: 2   AYDYGSILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITG---FFAPCSHSLLSNH 61
           A D GSILQFAP QSAVDEGFWHRLSSLKLNQLGIDDSPIPITG   FF P S       
Sbjct: 3   ANDKGSILQFAPFQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGLFLFFFPFSFFY---- 62

Query: 62  LTLLSESLPIEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKI 121
                 SLP EV+RDSSTPLTTKGNRNRCAVPGILYNTNTVESFHAL++LSLLKSEA KI
Sbjct: 63  ------SLPTEVKRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALEKLSLLKSEAKKI 122

Query: 122 WEDIRSGKALEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVVGLTSASQWFN 181
           WEDI SGKALEDSSVLARFLLISFADLK WNFHY FAFPALVLDPPATVV L SASQWFN
Sbjct: 123 WEDILSGKALEDSSVLARFLLISFADLKKWNFHYWFAFPALVLDPPATVVELKSASQWFN 182

Query: 182 FKEAESLFTAFGQWRSSDLTSDIPFFLVNIDSSSQASIKHLRDFETCQNNGGKLLFGFYD 241
            +EAESL TAFGQWRSSDLTSDIPFFLV IDS+SQASIKHLRDFETCQNNG KLLFGFYD
Sbjct: 183 LEEAESLSTAFGQWRSSDLTSDIPFFLVIIDSNSQASIKHLRDFETCQNNGEKLLFGFYD 242

Query: 242 PCHLPSNPGWPLRNFLALIYSKWNLKSVDFLCYRENRGFADLRLSLVGTALIDDPKGFRD 301
           PCHLPSNPGWPLRNFLALIYS+WNLKSV+FLCYRENRGFADL LSLVG ALIDDPKG RD
Sbjct: 243 PCHLPSNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDDPKGLRD 302

Query: 302 PSCMPNPVGWELKRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSS 361
             CMPN VGWEL +GKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNIN+LSS
Sbjct: 303 QCCMPNAVGWELNKGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINILSS 362

Query: 362 LKCLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAE 421
           LKCLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGG+FKAE
Sbjct: 363 LKCLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGDFKAE 422

Query: 422 AAVKSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAIFLLTDT 481
           AAVKSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDA+FLLTDT
Sbjct: 423 AAVKSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAVFLLTDT 482

Query: 482 RESRWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSWHDSTSQTNIANLSLNTTNT 541
           RESRWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGS HDSTSQTNIANLSLN TNT
Sbjct: 483 RESRWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSCHDSTSQTNIANLSLNATNT 542

Query: 542 RQRLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAE 601
           RQRLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAE
Sbjct: 543 RQRLGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAE 602

Query: 602 GELLNSGIAGASEQPLGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMD 661
           GELLNSGI GASEQPLGILPHQIRGF SQFSQMTLVGRSS+SCTACSSMVVSEYRNRGMD
Sbjct: 603 GELLNSGIDGASEQPLGILPHQIRGFLSQFSQMTLVGRSSNSCTACSSMVVSEYRNRGMD 662

Query: 662 FILQAINHPTYLEDLTGLTELMKSTSSFQLDWDNDSDGSGDDDGCIEI 706
           FILQAINHPTYLEDLTGLTELMKSTSSFQLDWDND+DGSGDDDGCIEI
Sbjct: 663 FILQAINHPTYLEDLTGLTELMKSTSSFQLDWDNDTDGSGDDDGCIEI 700

BLAST of CsGy6G034860 vs. ExPASy TrEMBL
Match: A0A6J1EF00 (Ubiquitin-like modifier-activating enzyme ATG7 OS=Cucurbita moschata OX=3662 GN=LOC111433608 PE=3 SV=1)

HSP 1 Score: 1262 bits (3266), Expect = 0.0
Identity = 630/706 (89.24%), Postives = 661/706 (93.63%), Query Frame = 0

Query: 2   AYDYGSILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSLLSNHLTL 61
           A D GSILQFAP QS VDEGFWHRLSSLKLN+LGIDDSPIPITGFFAPC+HS +SNHLTL
Sbjct: 3   ANDKGSILQFAPFQSLVDEGFWHRLSSLKLNELGIDDSPIPITGFFAPCAHSQVSNHLTL 62

Query: 62  LSESLPIEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKIWED 121
           LSESLP EV  +SS PLTTKGNRNRC+VPGILYNTNTVESFHALD+LSLLKSEA KIWED
Sbjct: 63  LSESLPSEVSSESSAPLTTKGNRNRCSVPGILYNTNTVESFHALDKLSLLKSEAKKIWED 122

Query: 122 IRSGKALEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVVGLTSASQWFNFKE 181
           I SGKA+EDSSVLARFLLISFADLK WNFHY FAFPALVL+PPATVV L  AS WFN +E
Sbjct: 123 ILSGKAIEDSSVLARFLLISFADLKKWNFHYWFAFPALVLNPPATVVELKPASHWFNLEE 182

Query: 182 AESLFTAFGQWRSSDLTSDIPFFLVNIDSSSQASIKHLRDFETCQNNGGKLLFGFYDPCH 241
           AES+ ++F QWRSS+LTSDIPFFLVNIDSSSQASIKHLRDFE CQNNG KLLFGFYDPCH
Sbjct: 183 AESVSSSFAQWRSSNLTSDIPFFLVNIDSSSQASIKHLRDFEACQNNGEKLLFGFYDPCH 242

Query: 242 LPSNPGWPLRNFLALIYSKWNLKSVDFLCYRENRGFADLRLSLVGTALIDDPKGFRDPSC 301
           LP+NPGWPLRNFLALIYS+WNLKSV+FLCYRENRGFADL LSLVG ALIDD +GFRD  C
Sbjct: 243 LPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDDTQGFRDQQC 302

Query: 302 MPNPVGWELKRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSSLKC 361
           MPN VGWEL RGKK +K+INLAKSMDPTRLAISAADLNLRLMRWRALPSLNIN+LSSLKC
Sbjct: 303 MPNVVGWELNRGKKCHKSINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINILSSLKC 362

Query: 362 LLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAEAAV 421
           LLLGAGTLGCQVARMLMAWG+RKITLVDSGRVAMSNPLRQSLYT+EDCLNGG+FKAEAAV
Sbjct: 363 LLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTMEDCLNGGDFKAEAAV 422

Query: 422 KSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAIFLLTDTRES 481
           KSLNRIFPAMEAEGVVISIPMPGHPV D+EAAS +DDCRRLDDLINSHDA+FLLTDTRES
Sbjct: 423 KSLNRIFPAMEAEGVVISIPMPGHPVSDNEAASMLDDCRRLDDLINSHDAVFLLTDTRES 482

Query: 482 RWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSWHDSTSQTNIANLSLNTTNTRQR 541
           RWLPTLLCAN NKVTITAALGFDSFLVMRHGAGPSGS HDSTSQTNIANLSLN  NT+QR
Sbjct: 483 RWLPTLLCANTNKVTITAALGFDSFLVMRHGAGPSGSCHDSTSQTNIANLSLNAANTKQR 542

Query: 542 LGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGEL 601
           LGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAE EL
Sbjct: 543 LGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAECEL 602

Query: 602 LNSGIAGASEQPLGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMDFIL 661
            NSG   ASEQPLGILPHQIRG  SQFSQMTLVGRSS++CTACSS+VVSEYRNRGMDFIL
Sbjct: 603 SNSG---ASEQPLGILPHQIRGSLSQFSQMTLVGRSSNTCTACSSLVVSEYRNRGMDFIL 662

Query: 662 QAINHPTYLEDLTGLTELMKSTSSFQLDWDNDSDGSGDDD-GCIEI 706
           QAINHPTYLEDLTGLTELMKSTSSFQLDWDN +DGSGDDD GCIEI
Sbjct: 663 QAINHPTYLEDLTGLTELMKSTSSFQLDWDNYTDGSGDDDDGCIEI 705

BLAST of CsGy6G034860 vs. ExPASy TrEMBL
Match: A0A6J1KW24 (Ubiquitin-like modifier-activating enzyme ATG7 OS=Cucurbita maxima OX=3661 GN=LOC111496942 PE=3 SV=1)

HSP 1 Score: 1257 bits (3252), Expect = 0.0
Identity = 628/707 (88.83%), Postives = 659/707 (93.21%), Query Frame = 0

Query: 2   AYDYGSILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSLLSNHLTL 61
           A D GSILQFAP QS VDEGFWHRLSSLKLN+LGIDDSPIPITGFFAPC+HS +SNHLTL
Sbjct: 3   ANDKGSILQFAPFQSLVDEGFWHRLSSLKLNELGIDDSPIPITGFFAPCAHSQVSNHLTL 62

Query: 62  LSESLPIEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKIWED 121
           LSESL  EV  +SS PLTTKGNRNRC+VPGILYNTNTVESFHALD+LSLLKSEA KIWED
Sbjct: 63  LSESLASEVSSESSAPLTTKGNRNRCSVPGILYNTNTVESFHALDKLSLLKSEAKKIWED 122

Query: 122 IRSGKALEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVVGLTSASQWFNFKE 181
           I SGKA+EDSSVLARFLLISFADLK WNFHY FAFPALVL+PPATVV L  AS WFN +E
Sbjct: 123 ILSGKAIEDSSVLARFLLISFADLKKWNFHYWFAFPALVLNPPATVVELKPASHWFNLEE 182

Query: 182 AESLFTAFGQWRSSDLTSDIPFFLVNIDSSSQASIKHLRDFETCQNNGGKLLFGFYDPCH 241
           AES+ ++F QWRSS+LTSD PFFLVNIDSSSQASIKHLRDFE C+NNG KLLFGFYDPCH
Sbjct: 183 AESVSSSFAQWRSSNLTSDTPFFLVNIDSSSQASIKHLRDFEACKNNGEKLLFGFYDPCH 242

Query: 242 LPSNPGWPLRNFLALIYSKWNLKSVDFLCYRENRGFADLRLSLVGTALIDDPKGFRDPSC 301
           LP+NPGWPLRNFLALIYS+WNLKSV+FLCYRENRGFADL LSLVG ALIDD +GFRD  C
Sbjct: 243 LPNNPGWPLRNFLALIYSRWNLKSVNFLCYRENRGFADLTLSLVGKALIDDTQGFRDQQC 302

Query: 302 MPNPVGWELKRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSSLKC 361
           MPN VGWEL RGKK +K+INLAKSMDPTRLAISAADLNLRLMRWRALPSLNIN+LSSLKC
Sbjct: 303 MPNVVGWELNRGKKCHKSINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINILSSLKC 362

Query: 362 LLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAEAAV 421
           LLLGAGTLGCQVARMLMAWG+RKITLVDSGRVAMSNPLRQSLYT+EDCLNGG FKAEAAV
Sbjct: 363 LLLGAGTLGCQVARMLMAWGIRKITLVDSGRVAMSNPLRQSLYTMEDCLNGGEFKAEAAV 422

Query: 422 KSLNRIFPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAIFLLTDTRES 481
           KSLNRIFPAMEAEGVVISIPMPGHPV D+EAAS +DDCRRLDDLINSHDA+FLLTDTRES
Sbjct: 423 KSLNRIFPAMEAEGVVISIPMPGHPVSDNEAASMLDDCRRLDDLINSHDAVFLLTDTRES 482

Query: 482 RWLPTLLCANANKVTITAALGFDSFLVMRHGAGPSGSWHDSTSQTNIANLSLNTTNTRQR 541
           RWLPTLLCAN NKVTITAALGFDSFLVMRHGAGPSGS HDSTSQTNIANLSLN TNT+QR
Sbjct: 483 RWLPTLLCANTNKVTITAALGFDSFLVMRHGAGPSGSCHDSTSQTNIANLSLNATNTKQR 542

Query: 542 LGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGEL 601
           LGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAE EL
Sbjct: 543 LGCYFCNDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAECEL 602

Query: 602 LNSGIAGASEQPLGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMDFIL 661
            NSG   ASEQPLGILPHQIRG  SQFSQMTLVGRSS++CTACSS+VVSEYRNRGMDFIL
Sbjct: 603 SNSG---ASEQPLGILPHQIRGSLSQFSQMTLVGRSSNTCTACSSLVVSEYRNRGMDFIL 662

Query: 662 QAINHPTYLEDLTGLTELMKSTSSFQLDWDNDSDGSGDDD--GCIEI 706
           QAINHPTYLEDLTGLTELMKSTSSFQLDWDN +DGSGDDD  GCIEI
Sbjct: 663 QAINHPTYLEDLTGLTELMKSTSSFQLDWDNYTDGSGDDDDDGCIEI 706

BLAST of CsGy6G034860 vs. TAIR 10
Match: AT5G45900.1 (ThiF family protein )

HSP 1 Score: 962.6 bits (2487), Expect = 1.7e-280
Identity = 477/702 (67.95%), Postives = 564/702 (80.34%), Query Frame = 0

Query: 8   ILQFAPLQSAVDEGFWHRLSSLKLNQLGIDDSPIPITGFFAPCSHSLLSNHLTLLSESLP 67
           ILQFAPL S+VDEGFWH  SSLKL++LGIDDSPI ITGF+ PC H  +SNHLTLLSESLP
Sbjct: 10  ILQFAPLNSSVDEGFWHSFSSLKLDKLGIDDSPISITGFYGPCGHPQVSNHLTLLSESLP 69

Query: 68  IEVRRDSSTPLTTKGNRNRCAVPGILYNTNTVESFHALDRLSLLKSEANKIWEDIRSGKA 127
           ++    S    T+ GNRN+C VPGILYNTNTVESF+ LD+ SLLK+EANKIWEDI+SGKA
Sbjct: 70  LD--EQSLIASTSHGNRNKCPVPGILYNTNTVESFNKLDKQSLLKAEANKIWEDIQSGKA 129

Query: 128 LEDSSVLARFLLISFADLKIWNFHYCFAFPALVLDPPATVVGLTSASQWFNFKEAESLFT 187
           LED SVL RFL+ISFADLK W+F Y FAFPA VLDPP +++ L  AS++F+ +EAES+  
Sbjct: 130 LEDPSVLPRFLVISFADLKKWSFRYWFAFPAFVLDPPVSLIELKPASEYFSSEEAESVSA 189

Query: 188 AFGQWRSSDLTSDIPFFLVNIDSSSQASIKHLRDFETCQNNGGKLLFGFYDPCHLPSNPG 247
           A   WR SDLT+D+PFFLV++ S S+ASI+HL+D E CQ +  KLLFGFYDPCHLPSNPG
Sbjct: 190 ACNDWRDSDLTTDVPFFLVSVSSDSKASIRHLKDLEACQGDHQKLLFGFYDPCHLPSNPG 249

Query: 248 WPLRNFLALIYSKWNLKSVDFLCYRENRGFADLRLSLVGTALIDDPKGFRDPSCMPNPVG 307
           WPLRN+LALI S+WNL++V F CYRE+RGFADL LSLVG A I    G      +PN VG
Sbjct: 250 WPLRNYLALIRSRWNLETVWFFCYRESRGFADLNLSLVGQASITLSSG-ESAETVPNSVG 309

Query: 308 WELKRGKKFYKTINLAKSMDPTRLAISAADLNLRLMRWRALPSLNINMLSSLKCLLLGAG 367
           WEL +GK+  ++I+LA SMDPTRLA+SA DLNL+LMRWRALPSLN+N+LSS+KCLLLGAG
Sbjct: 310 WELNKGKRVPRSISLANSMDPTRLAVSAVDLNLKLMRWRALPSLNLNVLSSVKCLLLGAG 369

Query: 368 TLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLEDCLNGGNFKAEAAVKSLNRI 427
           TLGCQVAR LM WG+R IT VD G+VAMSNP+RQSLY  EDCL  G FKA AAVKSL +I
Sbjct: 370 TLGCQVARTLMGWGIRNITFVDYGKVAMSNPVRQSLYNFEDCLGRGEFKAVAAVKSLKQI 429

Query: 428 FPAMEAEGVVISIPMPGHPVPDHEAASTIDDCRRLDDLINSHDAIFLLTDTRESRWLPTL 487
           FPAME  GVV++IPMPGHP+   E  S + DC+RL +LI SHDA+FLLTDTRESRWLP+L
Sbjct: 430 FPAMETSGVVMAIPMPGHPISSQEEDSVLGDCKRLSELIESHDAVFLLTDTRESRWLPSL 489

Query: 488 LCANANKVTITAALGFDSFLVMRHGAGPSGSWHDSTSQTNIANLSLNTTNTRQRLGCYFC 547
           LCANANK+ I AALGFDS++VMRHGAGP+    D      + NL +N TNT QRLGCYFC
Sbjct: 490 LCANANKIAINAALGFDSYMVMRHGAGPTSLSDD------MQNLDINKTNT-QRLGCYFC 549

Query: 548 NDVVAPIDSTANRTLDQQCTVTRPGLAPIASAIAVELLVGILHHPEGIYAEGE---LLNS 607
           NDVVAP DS  +RTLDQQCTVTRPGLAPIA A+AVELLVG+L HP GI A+G+   L N+
Sbjct: 550 NDVVAPQDSMTDRTLDQQCTVTRPGLAPIAGALAVELLVGVLQHPLGINAKGDNSSLSNT 609

Query: 608 GIAGASEQPLGILPHQIRGFFSQFSQMTLVGRSSDSCTACSSMVVSEYRNRGMDFILQAI 667
           G     + PLGILPHQIRG  SQFSQ+TL+G++S+SCTACS  V+SEYR RG  FIL+AI
Sbjct: 610 G--NNDDSPLGILPHQIRGSVSQFSQITLLGQASNSCTACSETVISEYRERGNSFILEAI 669

Query: 668 NHPTYLEDLTGLTELMKSTSSFQLDWDNDSDGSGDDDGCIEI 707
           NHPTYLEDLTGLTEL K+ +SF LDW++D   + DDD  +++
Sbjct: 670 NHPTYLEDLTGLTELKKAANSFNLDWEDDD--TDDDDVAVDL 697

BLAST of CsGy6G034860 vs. TAIR 10
Match: AT5G19180.1 (E1 C-terminal related 1 )

HSP 1 Score: 47.4 bits (111), Expect = 5.6e-05
Identity = 26/74 (35.14%), Postives = 43/74 (58.11%), Query Frame = 0

Query: 349 PSLNINMLSSLKCLLLGAGTLGCQVARMLMAWGVRKITLVDSGRVAMSNPLRQSLYTLED 408
           P L  ++   ++ L++GAG LGC++ + L   G R + ++D  R+ ++N  RQ L+ +ED
Sbjct: 37  PGLRDDIRDYVRILVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRIED 96

Query: 409 CLNGGNFKAEAAVK 423
               G  KAE A K
Sbjct: 97  V---GKPKAEVAAK 107

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94CD52.4e-27967.95Ubiquitin-like modifier-activating enzyme atg7 OS=Arabidopsis thaliana OX=3702 G... [more]
O953523.3e-15945.22Ubiquitin-like modifier-activating enzyme ATG7 OS=Homo sapiens OX=9606 GN=ATG7 P... [more]
Q5ZKY25.6e-15944.92Ubiquitin-like modifier-activating enzyme ATG7 OS=Gallus gallus OX=9031 GN=ATG7 ... [more]
Q641Y57.6e-15644.89Ubiquitin-like modifier-activating enzyme ATG7 OS=Rattus norvegicus OX=10116 GN=... [more]
Q9D9061.4e-15444.59Ubiquitin-like modifier-activating enzyme ATG7 OS=Mus musculus OX=10090 GN=Atg7 ... [more]
Match NameE-valueIdentityDescription
XP_011658247.10.0100.00ubiquitin-like modifier-activating enzyme atg7 [Cucumis sativus] >KGN49301.1 hyp... [more]
XP_008439746.10.094.33PREDICTED: ubiquitin-like modifier-activating enzyme atg7 [Cucumis melo][more]
XP_038880974.10.091.58ubiquitin-like modifier-activating enzyme atg7 [Benincasa hispida][more]
KAA0052623.10.092.09ubiquitin-like modifier-activating enzyme atg7 [Cucumis melo var. makuwa] >TYK13... [more]
XP_022926466.10.089.24ubiquitin-like modifier-activating enzyme atg7 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0KNJ90.0100.00Ubiquitin-like modifier-activating enzyme ATG7 OS=Cucumis sativus OX=3659 GN=Csa... [more]
A0A1S3AZ280.094.33Ubiquitin-like modifier-activating enzyme ATG7 OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A5D3CNQ10.092.09Ubiquitin-like modifier-activating enzyme ATG7 OS=Cucumis melo var. makuwa OX=11... [more]
A0A6J1EF000.089.24Ubiquitin-like modifier-activating enzyme ATG7 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1KW240.088.83Ubiquitin-like modifier-activating enzyme ATG7 OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT5G45900.11.7e-28067.95ThiF family protein [more]
AT5G19180.15.6e-0535.14E1 C-terminal related 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000594THIF-type NAD/FAD binding foldPFAMPF00899ThiFcoord: 354..641
e-value: 1.6E-40
score: 139.0
IPR006285Ubiquitin-like modifier-activating enzyme Atg7TIGRFAMTIGR01381TIGR01381coord: 11..695
e-value: 3.7E-271
score: 899.3
IPR032197Ubiquitin-like modifier-activating enzyme Atg7, N-terminalPFAMPF16420ATG7_Ncoord: 9..326
e-value: 7.2E-89
score: 298.4
NoneNo IPR availableGENE3D3.40.50.720coord: 326..701
e-value: 1.5E-138
score: 463.1
NoneNo IPR availablePIRSRPIRSR606285-1PIRSR606285-1coord: 8..695
e-value: 2.9E-231
score: 767.3
NoneNo IPR availablePANTHERPTHR10953:SF3UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME ATG7coord: 9..693
NoneNo IPR availablePANTHERPTHR10953UBIQUITIN-ACTIVATING ENZYME E1coord: 9..693
NoneNo IPR availableCDDcd01486Apg7coord: 360..676
e-value: 0.0
score: 516.926
IPR042522Ubiquitin-like modifier-activating enzyme Atg7, N-terminal, subdomain 1GENE3D3.40.140.70coord: 14..322
e-value: 1.5E-114
score: 384.3
IPR042523Ubiquitin-like modifier-activating enzyme Atg7, N-terminal, subdomain 2GENE3D3.40.140.100coord: 9..292
e-value: 1.5E-114
score: 384.3
IPR035985Ubiquitin-activating enzymeSUPERFAMILY69572Activating enzymes of the ubiquitin-like proteinscoord: 348..671

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy6G034860.2CsGy6G034860.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000045 autophagosome assembly
biological_process GO:0000422 autophagy of mitochondrion
biological_process GO:0006995 cellular response to nitrogen starvation
biological_process GO:0006501 C-terminal protein lipidation
biological_process GO:0050832 defense response to fungus
biological_process GO:0044805 late nucleophagy
biological_process GO:0010150 leaf senescence
biological_process GO:0034727 piecemeal microautophagy of the nucleus
biological_process GO:0032446 protein modification by small protein conjugation
biological_process GO:0015031 protein transport
cellular_component GO:0000407 phagophore assembly site
cellular_component GO:0005737 cytoplasm
molecular_function GO:0019778 Atg12 activating enzyme activity
molecular_function GO:0019779 Atg8 activating enzyme activity
molecular_function GO:0008641 ubiquitin-like modifier activating enzyme activity