CsGy6G033800 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy6G033800
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionvesicle-associated protein 4-2
LocationGy14Chr6: 30287077 .. 30290892 (+)
RNA-Seq ExpressionCsGy6G033800
SyntenyCsGy6G033800
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTCTCTCTCTTTCTCTCTCTCTCTCCACCTCGTCTTCCGCTTCCATTGAACTAAAAAGCGACCCCTCCTCCTCTTAACGCCCATCATCAACTGCCGTGGCTCCTCCTCCTCAATTCCCATCTCCAACCACCACTTTCTTTACTGTCCGATGCTCTGATTGAGTCTCGGAGGTTTCAATGATGGCTGTTGAAGACCAAAAGTCAACGTCCGATGGAAAGGTCTGGGGTTTTTTGAAGCAAACGTTTAAGCATTCCACTGGCGGTGCTACTACTTCCTCTCGTACTTCTCATCAAACTCATCCGCCTGTCGAGGGGACCAATCCTCATACCTCCGCTTCTGTCTCCTCTGTTGCTAGGTCGCTGCTTCCGACGCGGCGCCGGCTCAAGCTTGATCCATCGAATAAGCTTTTCTTTCCTTGTGAGTTTTTTTTTCTTTTTTTTTTTTTTACTTTATTTGTTATATGAATTTTGTTCGATTTTTTTAGTTGGATTTGTTCTGTTCATTCTAGGTCTAGTGCCTGTGATCTCGATCTGCTGTTGACTTTTAGCTATCAGCTACATTTCGGCTTTAGACAGCTGGAATTCTCTAATTGATTTGATTTTCTGGTTGATCTTTCTTTTAATTGATTCGTCTTTCTGTTTTTGAAGATGAGCCTGGTAAGCAGGTTAGGAGTGCCATTGGAATAAAAAACACGAGCAAATCTTATGTTGCATTCAAGGTACGAATGAACGTGCTATTTGAGGCATACTGTTTTGCATATTTGTATCCATATTTCGAGCATACAGAATTTCTCTCTCACCGTTTTGTTAATCAGTGGACACTGGACAGTGAGTTCGTGTGCTAGTATTAATAGCTATAATTTCCAAAAATAGCTTTCCTTTTAATACTGTTTGTAGAATTCGGCTTATCGTGATTATTGATTCATTGTGTTTGAACTTTCATACAGTTCCAAACAACTGCACCAAAAAGCTGCTTCATGCGTCCTCCTGGTGCAATTCTTGCCCCTGGAGAGAGCATCATAGCCACTGGTATTCATTTTGTCTCTGTTATTAAGTTCATATGTAACAGCTTTGATTGTTAATTATGCTTATTTTCTTATATCATCATATTATTATATATATATGTTTTTAACTGCTGTCGGTGTTGGATGGATCTTCTAGTTTTCAAGTTTGTTGAGGTTCCCGAAAGCAACGAGAAACCTGTGGATCAAAAGAACAACAGGGTTAAGTTTAAAATTATGAGCTTGAAGGTGAAAGGACAAATGGATTATGTGCCTGAACTGGTGCGTCTCAATCTTCCCTTAGCTCGCTTTTCCAATTATTTACGTTTTGTGGGATGTTTTATTCAACAAGTTAATTAGGTCTTAGTTACAAATGTTCAGTTTTGTCTTGTTTCACTTTAACTTGATGAGTCCTTAAGAGGAAACGGAATTGTCAATATCTAGACTCTAAAGTAGTTTCAGCCGACACTTGTCAAAATCAGAGGGCTATTTTCATCTGTGAGCCAGGTTTGGATGCTGTTTTAATTACAAGTGCCACACTGAAAGGAGTATTGCTTGCGCACAAGAGTTTCCAATTCCGTACAAGAGTTTAGGCTGATAATTTGATGATCGTTAAACTTTTGTTGGATATTCTCAGCACAATATGCGTAACTTACTGTCGCTGGCATCCTATTGCATACTTTAATTTTGTTTCTAAGAGTCTTGGCAATTTTCTTTTCCTGGTTAGGATGTGTAGTGATAAGTCCATTAAGGTTATATACAAACTTTTTGGCCTTCTCTTGAATGGTGTAAATGTGTTTCTTTCCGATTTTTACAGATTAGTTCTCAAATGACCTAATTAACTGGAGTGCGTTTTTATAACCCCAGTTTTGGTTTTCTGCCTTCCTGTGTATTTCGTTTACCCGATGACTTAAAGATAAACTTCAAATTTATGAATGTAGTATTCATTCATTGCTTGTTCTAAAATGTTCGTATCTGACTAGAAAGTGCAAGTAAAATGAGTACTGATCAAGCATTCTTTTTCTATCTTGTTCTAGTTTGATGAGCAGAAGGACCAAGTGTCTATAGAACAGATATTGCGGGTTGTTTTCCTGGATCCAGAACGTCCCAATGCAGTAAGTTACCTCCTTGGATGATGATAAGAATAAAAATTTCACCAACCCTCCCAATATAATCAAATATTTCGTGTAAAGTTGAAAATTATTTTACCTTTCTGTCCTTTTTGGAATGAGAGATTTTGTGATGAATATAATTAAAACCTCTTTGTTTAGAAGTTCACAGAAAAGGAAAAGAAAATGAGAAACTTACTAGTATAGCCACAATCTAGTTCGAAATATTGTGATTTGAGGCATAAGTAGTAATCCCTTTCATATATGATTCTCTCAAGGCTTTAGAAAAATTGAAGCGCCAATTAGCGGAAGCCGATGCTGCACTTGAGGCACGCAAGAAACCACCGGAGGAATCGGGCCCAAAAATAATTGGAGAAGGGCTGGTGATAGACGAATGGGTGAGCTTATTATCCTTAATTTTATAATTCAAATCTAATAATATTTTGAATGTAATGAATATCACTCTTAATGTTACTTCTTCCATTTTGTGGCTTTTCAGAAAGAACGGAGGGAAAGATATCTGGCAAAACAGCAAGTTGAAGGGGTAGATTCTGTATAGGTTTGGTTTTAGTTTTTTTTTTCTCCCGGCAATGCTTCTGTAATTTGAAAGGGATTTCCAAATGAACTCTCGAGTATCGATTCTACCCTTAAAAAGTCATGTAATCTTTTTCTTTTGGTATATTATCCAAAATATTGTTTAGGTGAAGATGAACTTGTTTTATTTTCATTGAACGAAACCCCCCAAGGTCAAGTCTTGTGTAATTTGGGACACCGACTGGGATTACTCCTTTTATATGTAGATAAAGATAATATGATTTGTTTCTTTCTCGCTAATAAATTGTCTGAGGTG

mRNA sequence

CTCTCTCTCTCTTTCTCTCTCTCTCTCCACCTCGTCTTCCGCTTCCATTGAACTAAAAAGCGACCCCTCCTCCTCTTAACGCCCATCATCAACTGCCGTGGCTCCTCCTCCTCAATTCCCATCTCCAACCACCACTTTCTTTACTGTCCGATGCTCTGATTGAGTCTCGGAGGTTTCAATGATGGCTGTTGAAGACCAAAAGTCAACGTCCGATGGAAAGGTCTGGGGTTTTTTGAAGCAAACGTTTAAGCATTCCACTGGCGGTGCTACTACTTCCTCTCGTACTTCTCATCAAACTCATCCGCCTGTCGAGGGGACCAATCCTCATACCTCCGCTTCTGTCTCCTCTGTTGCTAGGTCGCTGCTTCCGACGCGGCGCCGGCTCAAGCTTGATCCATCGAATAAGCTTTTCTTTCCTTATGAGCCTGGTAAGCAGGTTAGGAGTGCCATTGGAATAAAAAACACGAGCAAATCTTATGTTGCATTCAAGTTCCAAACAACTGCACCAAAAAGCTGCTTCATGCGTCCTCCTGGTGCAATTCTTGCCCCTGGAGAGAGCATCATAGCCACTGTTTTCAAGTTTGTTGAGGTTCCCGAAAGCAACGAGAAACCTGTGGATCAAAAGAACAACAGGGTTAAGTTTAAAATTATGAGCTTGAAGGTGAAAGGACAAATGGATTATGTGCCTGAACTGTTTGATGAGCAGAAGGACCAAGTGTCTATAGAACAGATATTGCGGGTTGTTTTCCTGGATCCAGAACGTCCCAATGCAGCTTTAGAAAAATTGAAGCGCCAATTAGCGGAAGCCGATGCTGCACTTGAGGCACGCAAGAAACCACCGGAGGAATCGGGCCCAAAAATAATTGGAGAAGGGCTGGTGATAGACGAATGGAAAGAACGGAGGGAAAGATATCTGGCAAAACAGCAAGTTGAAGGGGTAGATTCTGTATAGGTTTGGTTTTAGTTTTTTTTTTCTCCCGGCAATGCTTCTGTAATTTGAAAGGGATTTCCAAATGAACTCTCGAGTATCGATTCTACCCTTAAAAAGTCATGTAATCTTTTTCTTTTGGTATATTATCCAAAATATTGTTTAGGTGAAGATGAACTTGTTTTATTTTCATTGAACGAAACCCCCCAAGGTCAAGTCTTGTGTAATTTGGGACACCGACTGGGATTACTCCTTTTATATGTAGATAAAGATAATATGATTTGTTTCTTTCTCGCTAATAAATTGTCTGAGGTG

Coding sequence (CDS)

ATGATGGCTGTTGAAGACCAAAAGTCAACGTCCGATGGAAAGGTCTGGGGTTTTTTGAAGCAAACGTTTAAGCATTCCACTGGCGGTGCTACTACTTCCTCTCGTACTTCTCATCAAACTCATCCGCCTGTCGAGGGGACCAATCCTCATACCTCCGCTTCTGTCTCCTCTGTTGCTAGGTCGCTGCTTCCGACGCGGCGCCGGCTCAAGCTTGATCCATCGAATAAGCTTTTCTTTCCTTATGAGCCTGGTAAGCAGGTTAGGAGTGCCATTGGAATAAAAAACACGAGCAAATCTTATGTTGCATTCAAGTTCCAAACAACTGCACCAAAAAGCTGCTTCATGCGTCCTCCTGGTGCAATTCTTGCCCCTGGAGAGAGCATCATAGCCACTGTTTTCAAGTTTGTTGAGGTTCCCGAAAGCAACGAGAAACCTGTGGATCAAAAGAACAACAGGGTTAAGTTTAAAATTATGAGCTTGAAGGTGAAAGGACAAATGGATTATGTGCCTGAACTGTTTGATGAGCAGAAGGACCAAGTGTCTATAGAACAGATATTGCGGGTTGTTTTCCTGGATCCAGAACGTCCCAATGCAGCTTTAGAAAAATTGAAGCGCCAATTAGCGGAAGCCGATGCTGCACTTGAGGCACGCAAGAAACCACCGGAGGAATCGGGCCCAAAAATAATTGGAGAAGGGCTGGTGATAGACGAATGGAAAGAACGGAGGGAAAGATATCTGGCAAAACAGCAAGTTGAAGGGGTAGATTCTGTATAG

Protein sequence

MMAVEDQKSTSDGKVWGFLKQTFKHSTGGATTSSRTSHQTHPPVEGTNPHTSASVSSVARSLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGAILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQVSIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKERRERYLAKQQVEGVDSV*
Homology
BLAST of CsGy6G033800 vs. ExPASy Swiss-Prot
Match: Q8VYN2 (Vesicle-associated protein 4-2 OS=Arabidopsis thaliana OX=3702 GN=PVA42 PE=1 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 4.1e-99
Identity = 203/296 (68.58%), Postives = 227/296 (76.69%), Query Frame = 0

Query: 2   MAVEDQKSTSDGK-VWGFLKQTFKHSTG---------------GATTSSRTS-------- 61
           M + ++K TSDGK  WGF K  F++S+G               GA++SS +S        
Sbjct: 1   MTMTEEKPTSDGKGGWGFFKIPFRNSSGHRNAASSAATSPFPSGASSSSTSSHLHNHHQH 60

Query: 62  -----HQTHPPVEGTNPH-----------TSASVSSVARSLLPTRRRLKLDPSNKLFFPY 121
                HQ H  +    PH            S SVSSVA+S LPT+RRLKLDPS KL+FPY
Sbjct: 61  HHQHHHQHHHQLGYNGPHGDGSGQNQHPTPSPSVSSVAKSFLPTKRRLKLDPSEKLYFPY 120

Query: 122 EPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGAILAPGESIIATVFKFVEVPES 181
           EPGKQVRSAI IKNTSKS+VAFKFQTTAPKSCFMRPPGAILAPGE+IIATVFKFVE PE+
Sbjct: 121 EPGKQVRSAIKIKNTSKSHVAFKFQTTAPKSCFMRPPGAILAPGETIIATVFKFVEPPEN 180

Query: 182 NEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQVSIEQILRVVFLDPERPNAALE 241
           NEKP+DQ+ +RVKFKIMSLKVKG MDYVPELFDEQKD VS EQILRV+FLDPER N ALE
Sbjct: 181 NEKPMDQR-SRVKFKIMSLKVKGPMDYVPELFDEQKDDVSKEQILRVIFLDPERSNPALE 240

Query: 242 KLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKERRERYLAKQQVEGVDSV 258
           KLKRQLAEADAA+EARKKPPEE+GPK+IGEGLVIDEWKERRERYLA+QQ EG DSV
Sbjct: 241 KLKRQLAEADAAVEARKKPPEETGPKMIGEGLVIDEWKERRERYLAQQQGEGADSV 295

BLAST of CsGy6G033800 vs. ExPASy Swiss-Prot
Match: Q8LPQ7 (Vesicle-associated protein 4-3 OS=Arabidopsis thaliana OX=3702 GN=PVA43 PE=2 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 2.6e-85
Identity = 186/288 (64.58%), Postives = 213/288 (73.96%), Query Frame = 0

Query: 2   MAVEDQKSTSDGKVWGFLKQTFKHSTGGATTSSRTS-------------------HQTH- 61
           MA+ + KS SDG+ WG  K  F++S   A ++S +S                   HQ+  
Sbjct: 1   MALTEDKSDSDGRRWGKFKLPFRNSNSQAPSASSSSSMATSSSSVTSSHLNQNYIHQSRH 60

Query: 62  -----PPVE---GTNPHTSA----SVSSVARSLLPTRRRLKLDPSNKLFFPYEPGKQVRS 121
                PPV    G N H SA    S+SSVARSLLPT+RRLKLDPS KL+FPYEPGKQVRS
Sbjct: 61  FQYHGPPVVEGLGQNHHQSAATIPSMSSVARSLLPTKRRLKLDPSAKLYFPYEPGKQVRS 120

Query: 122 AIGIKNTSKSYVAFKFQTTAPKSCFMRPPGAILAPGESIIATVFKFVEVPESNEKPVDQK 181
           AI IKNTSKS+VAFKFQTT PKSCFMRP GAILAPGE IIATVFKFVE PE+NEKP++QK
Sbjct: 121 AIKIKNTSKSHVAFKFQTTVPKSCFMRPAGAILAPGEEIIATVFKFVEPPENNEKPMEQK 180

Query: 182 NNRVKFKIMSLKVKGQMDYVPELFDEQKDQVSIEQILRVVFLDPERPNAALEKLKRQLAE 241
           +  VKFKIMSLK+K   DY+PELF+EQKD VS EQ++RVVFLDPE PN+ +EKLK QLAE
Sbjct: 181 SG-VKFKIMSLKMKVPTDYMPELFEEQKDHVSEEQVMRVVFLDPENPNSMMEKLKSQLAE 240

Query: 242 ADAALEARKKPPEE-SGPKIIGEGLVIDEWKERRERYLAKQQVEGVDS 257
           ADAA EARKK  E   GPK IGEGLVIDEWK+RRERYLA+QQ  GVD+
Sbjct: 241 ADAADEARKKASEGIVGPKPIGEGLVIDEWKQRRERYLAQQQ-GGVDA 286

BLAST of CsGy6G033800 vs. ExPASy Swiss-Prot
Match: Q1ECE0 (Vesicle-associated protein 4-1 OS=Arabidopsis thaliana OX=3702 GN=PVA41 PE=2 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 5.7e-85
Identity = 163/243 (67.08%), Postives = 198/243 (81.48%), Query Frame = 0

Query: 16  WGFLKQTFKHSTGGATTSSRTSHQTHPPVEGTNPHTSASVSSVARSLLPTRRRLKLDPSN 75
           W     T   ST     + R+ H               ++SSVARSLLP RRRL+LDPS+
Sbjct: 24  WQRRSTTSSSSTQNPNQNYRSRHGNRNTDISAVSKPPLTMSSVARSLLPARRRLRLDPSS 83

Query: 76  KLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGAILAPGESIIATVFKF 135
            L+FPYEPGKQVRSAI +KNTSKS+ AFKFQTTAPKSC+MRPPG +LAPGES+ ATVFKF
Sbjct: 84  YLYFPYEPGKQVRSAIKLKNTSKSHTAFKFQTTAPKSCYMRPPGGVLAPGESVFATVFKF 143

Query: 136 VEVPESNEK-PVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQVSIEQILRVVFLDPE 195
           VE PE+NEK P++QK ++VKFKIMSLKVK  ++YVPELFDEQKDQV++EQ+LRV+F+D +
Sbjct: 144 VEHPENNEKQPLNQK-SKVKFKIMSLKVKPGVEYVPELFDEQKDQVAVEQVLRVIFIDAD 203

Query: 196 RPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKERRERYLAKQQVEGV 255
           RP+AALEKLKRQL EA+AA+EARKKPP E+GP+++GEGLVIDEWKERRE+YLA+QQVE V
Sbjct: 204 RPSAALEKLKRQLDEAEAAVEARKKPPPETGPRVVGEGLVIDEWKERREKYLARQQVESV 263

Query: 256 DSV 258
           DS+
Sbjct: 264 DSL 265

BLAST of CsGy6G033800 vs. ExPASy Swiss-Prot
Match: Q9SHC8 (Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 3.4e-05
Identity = 39/130 (30.00%), Postives = 63/130 (48.46%), Query Frame = 0

Query: 69  LKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGAILAPGESI 128
           L +DP + L FP+E  KQ+  ++ + N + +YVAFK +TT PK   +RP   ++ P  S 
Sbjct: 6   LTIDPVD-LQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHPRSSS 65

Query: 129 IATVFKFVEVPESNEKPVDQ--KNNRVKFKIMSLKVKGQMDYVPELFDEQKDQVSIEQIL 188
                  V +    E P D   K+  +   +++       D   E+F ++      E  L
Sbjct: 66  EV----LVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKL 125

Query: 189 RVVFLDPERP 197
           RVV++ P RP
Sbjct: 126 RVVYVAPPRP 130

BLAST of CsGy6G033800 vs. ExPASy Swiss-Prot
Match: Q9SYC9 (Vesicle-associated protein 1-4 OS=Arabidopsis thaliana OX=3702 GN=PVA14 PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 2.2e-04
Identity = 34/127 (26.77%), Postives = 63/127 (49.61%), Query Frame = 0

Query: 79  FPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGAILAPGESIIATVFKFVEV 138
           FP E  KQ   ++ + N + +YVAFK QTT PK   ++P   ++ P  S        V +
Sbjct: 16  FPIELNKQGSCSLNLTNKTDNYVAFKAQTTKPKMYCVKPSVGVVLPRSSCEV----LVVM 75

Query: 139 PESNEKPVD-QKNNRVKFKIMSLKVKGQMD--YVPELFDEQKDQVSIEQILRVVFLDPER 198
               E P D Q  +++ F+   ++  G MD     E+F ++      E I +++++ P +
Sbjct: 76  QALKEAPADRQCKDKLLFQCKVVE-PGTMDKEVTSEMFSKEAGHRVEETIFKIIYVAPPQ 135

Query: 199 PNAALEK 203
           P + +++
Sbjct: 136 PQSPVQE 137

BLAST of CsGy6G033800 vs. NCBI nr
Match: XP_004134732.2 (vesicle-associated protein 4-2 [Cucumis sativus] >KGN49181.1 hypothetical protein Csa_003551 [Cucumis sativus])

HSP 1 Score: 502 bits (1292), Expect = 6.05e-179
Identity = 257/257 (100.00%), Postives = 257/257 (100.00%), Query Frame = 0

Query: 1   MMAVEDQKSTSDGKVWGFLKQTFKHSTGGATTSSRTSHQTHPPVEGTNPHTSASVSSVAR 60
           MMAVEDQKSTSDGKVWGFLKQTFKHSTGGATTSSRTSHQTHPPVEGTNPHTSASVSSVAR
Sbjct: 1   MMAVEDQKSTSDGKVWGFLKQTFKHSTGGATTSSRTSHQTHPPVEGTNPHTSASVSSVAR 60

Query: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA 120
           SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA
Sbjct: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA 120

Query: 121 ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV 180
           ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV
Sbjct: 121 ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV 180

Query: 181 SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE 240
           SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE
Sbjct: 181 SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE 240

Query: 241 RRERYLAKQQVEGVDSV 257
           RRERYLAKQQVEGVDSV
Sbjct: 241 RRERYLAKQQVEGVDSV 257

BLAST of CsGy6G033800 vs. NCBI nr
Match: XP_008439916.1 (PREDICTED: vesicle-associated protein 4-2 [Cucumis melo])

HSP 1 Score: 496 bits (1278), Expect = 8.24e-177
Identity = 254/257 (98.83%), Postives = 256/257 (99.61%), Query Frame = 0

Query: 1   MMAVEDQKSTSDGKVWGFLKQTFKHSTGGATTSSRTSHQTHPPVEGTNPHTSASVSSVAR 60
           MMAVEDQKSTSDGKVWGFLKQTF+HST GATTSSRTSHQTHPPV+GTNPHTSASVSSVAR
Sbjct: 1   MMAVEDQKSTSDGKVWGFLKQTFRHSTTGATTSSRTSHQTHPPVDGTNPHTSASVSSVAR 60

Query: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA 120
           SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA
Sbjct: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA 120

Query: 121 ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV 180
           ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV
Sbjct: 121 ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV 180

Query: 181 SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE 240
           SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE
Sbjct: 181 SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE 240

Query: 241 RRERYLAKQQVEGVDSV 257
           RRERYLAKQQVEGVDSV
Sbjct: 241 RRERYLAKQQVEGVDSV 257

BLAST of CsGy6G033800 vs. NCBI nr
Match: XP_038883586.1 (vesicle-associated protein 4-2 [Benincasa hispida])

HSP 1 Score: 488 bits (1257), Expect = 1.31e-173
Identity = 249/257 (96.89%), Postives = 253/257 (98.44%), Query Frame = 0

Query: 1   MMAVEDQKSTSDGKVWGFLKQTFKHSTGGATTSSRTSHQTHPPVEGTNPHTSASVSSVAR 60
           MMA++DQKSTSDGKVWGFLKQTF+HS  GATTSSRTSHQTHPPVEGTNPHTSASVSSVAR
Sbjct: 1   MMAIDDQKSTSDGKVWGFLKQTFRHSASGATTSSRTSHQTHPPVEGTNPHTSASVSSVAR 60

Query: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA 120
           SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA
Sbjct: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA 120

Query: 121 ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV 180
           ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKG MDYVPELFDEQKDQV
Sbjct: 121 ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGPMDYVPELFDEQKDQV 180

Query: 181 SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE 240
           SIEQILRVVFLDPERPNAAL+KLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE
Sbjct: 181 SIEQILRVVFLDPERPNAALDKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE 240

Query: 241 RRERYLAKQQVEGVDSV 257
           RRERYLAKQQVE VDSV
Sbjct: 241 RRERYLAKQQVEEVDSV 257

BLAST of CsGy6G033800 vs. NCBI nr
Match: XP_022977981.1 (vesicle-associated protein 4-2-like [Cucurbita maxima] >XP_023543767.1 vesicle-associated protein 4-2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 483 bits (1244), Expect = 1.26e-171
Identity = 246/257 (95.72%), Postives = 252/257 (98.05%), Query Frame = 0

Query: 1   MMAVEDQKSTSDGKVWGFLKQTFKHSTGGATTSSRTSHQTHPPVEGTNPHTSASVSSVAR 60
           MMA++DQKSTSDGKVWGFLKQTF+HS  GATTS RTSHQTHPPVE TNPHTSASVSSVAR
Sbjct: 1   MMAIDDQKSTSDGKVWGFLKQTFRHSASGATTSYRTSHQTHPPVERTNPHTSASVSSVAR 60

Query: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA 120
           SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA
Sbjct: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA 120

Query: 121 ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV 180
           ILAPGES+IATVFKFVEVPE+NEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV
Sbjct: 121 ILAPGESLIATVFKFVEVPENNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV 180

Query: 181 SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE 240
           SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKP EE+GPKIIGEGLVIDEWKE
Sbjct: 181 SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPAEETGPKIIGEGLVIDEWKE 240

Query: 241 RRERYLAKQQVEGVDSV 257
           RRERYLAKQQVEGVDSV
Sbjct: 241 RRERYLAKQQVEGVDSV 257

BLAST of CsGy6G033800 vs. NCBI nr
Match: XP_022142290.1 (vesicle-associated protein 4-2 [Momordica charantia])

HSP 1 Score: 481 bits (1239), Expect = 7.26e-171
Identity = 246/257 (95.72%), Postives = 249/257 (96.89%), Query Frame = 0

Query: 1   MMAVEDQKSTSDGKVWGFLKQTFKHSTGGATTSSRTSHQTHPPVEGTNPHTSASVSSVAR 60
           MMA+EDQKSTSDGKVWGFLK TF+HS   ATTSSRTSH THPPVEGTNPH SASVSSVAR
Sbjct: 1   MMAIEDQKSTSDGKVWGFLKLTFRHSATSATTSSRTSHHTHPPVEGTNPHASASVSSVAR 60

Query: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA 120
           SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSY AFKFQTTAPKSCFMRPPGA
Sbjct: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYAAFKFQTTAPKSCFMRPPGA 120

Query: 121 ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV 180
           ILAPGESIIATVFKFVEVPE+NEKPVDQKNNRVKFKIMSLKVKG MDYVPELFDEQKDQV
Sbjct: 121 ILAPGESIIATVFKFVEVPENNEKPVDQKNNRVKFKIMSLKVKGPMDYVPELFDEQKDQV 180

Query: 181 SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE 240
           SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE
Sbjct: 181 SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE 240

Query: 241 RRERYLAKQQVEGVDSV 257
           RRERYLAKQQVEGVDSV
Sbjct: 241 RRERYLAKQQVEGVDSV 257

BLAST of CsGy6G033800 vs. ExPASy TrEMBL
Match: A0A0A0KN70 (MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G516890 PE=4 SV=1)

HSP 1 Score: 502 bits (1292), Expect = 2.93e-179
Identity = 257/257 (100.00%), Postives = 257/257 (100.00%), Query Frame = 0

Query: 1   MMAVEDQKSTSDGKVWGFLKQTFKHSTGGATTSSRTSHQTHPPVEGTNPHTSASVSSVAR 60
           MMAVEDQKSTSDGKVWGFLKQTFKHSTGGATTSSRTSHQTHPPVEGTNPHTSASVSSVAR
Sbjct: 1   MMAVEDQKSTSDGKVWGFLKQTFKHSTGGATTSSRTSHQTHPPVEGTNPHTSASVSSVAR 60

Query: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA 120
           SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA
Sbjct: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA 120

Query: 121 ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV 180
           ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV
Sbjct: 121 ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV 180

Query: 181 SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE 240
           SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE
Sbjct: 181 SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE 240

Query: 241 RRERYLAKQQVEGVDSV 257
           RRERYLAKQQVEGVDSV
Sbjct: 241 RRERYLAKQQVEGVDSV 257

BLAST of CsGy6G033800 vs. ExPASy TrEMBL
Match: A0A1S3AZX4 (vesicle-associated protein 4-2 OS=Cucumis melo OX=3656 GN=LOC103484561 PE=4 SV=1)

HSP 1 Score: 496 bits (1278), Expect = 3.99e-177
Identity = 254/257 (98.83%), Postives = 256/257 (99.61%), Query Frame = 0

Query: 1   MMAVEDQKSTSDGKVWGFLKQTFKHSTGGATTSSRTSHQTHPPVEGTNPHTSASVSSVAR 60
           MMAVEDQKSTSDGKVWGFLKQTF+HST GATTSSRTSHQTHPPV+GTNPHTSASVSSVAR
Sbjct: 1   MMAVEDQKSTSDGKVWGFLKQTFRHSTTGATTSSRTSHQTHPPVDGTNPHTSASVSSVAR 60

Query: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA 120
           SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA
Sbjct: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA 120

Query: 121 ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV 180
           ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV
Sbjct: 121 ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV 180

Query: 181 SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE 240
           SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE
Sbjct: 181 SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE 240

Query: 241 RRERYLAKQQVEGVDSV 257
           RRERYLAKQQVEGVDSV
Sbjct: 241 RRERYLAKQQVEGVDSV 257

BLAST of CsGy6G033800 vs. ExPASy TrEMBL
Match: A0A6J1ILI2 (vesicle-associated protein 4-2-like OS=Cucurbita maxima OX=3661 GN=LOC111478109 PE=4 SV=1)

HSP 1 Score: 483 bits (1244), Expect = 6.08e-172
Identity = 246/257 (95.72%), Postives = 252/257 (98.05%), Query Frame = 0

Query: 1   MMAVEDQKSTSDGKVWGFLKQTFKHSTGGATTSSRTSHQTHPPVEGTNPHTSASVSSVAR 60
           MMA++DQKSTSDGKVWGFLKQTF+HS  GATTS RTSHQTHPPVE TNPHTSASVSSVAR
Sbjct: 1   MMAIDDQKSTSDGKVWGFLKQTFRHSASGATTSYRTSHQTHPPVERTNPHTSASVSSVAR 60

Query: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA 120
           SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA
Sbjct: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA 120

Query: 121 ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV 180
           ILAPGES+IATVFKFVEVPE+NEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV
Sbjct: 121 ILAPGESLIATVFKFVEVPENNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV 180

Query: 181 SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE 240
           SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKP EE+GPKIIGEGLVIDEWKE
Sbjct: 181 SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPAEETGPKIIGEGLVIDEWKE 240

Query: 241 RRERYLAKQQVEGVDSV 257
           RRERYLAKQQVEGVDSV
Sbjct: 241 RRERYLAKQQVEGVDSV 257

BLAST of CsGy6G033800 vs. ExPASy TrEMBL
Match: A0A6J1CKI9 (vesicle-associated protein 4-2 OS=Momordica charantia OX=3673 GN=LOC111012445 PE=4 SV=1)

HSP 1 Score: 481 bits (1239), Expect = 3.51e-171
Identity = 246/257 (95.72%), Postives = 249/257 (96.89%), Query Frame = 0

Query: 1   MMAVEDQKSTSDGKVWGFLKQTFKHSTGGATTSSRTSHQTHPPVEGTNPHTSASVSSVAR 60
           MMA+EDQKSTSDGKVWGFLK TF+HS   ATTSSRTSH THPPVEGTNPH SASVSSVAR
Sbjct: 1   MMAIEDQKSTSDGKVWGFLKLTFRHSATSATTSSRTSHHTHPPVEGTNPHASASVSSVAR 60

Query: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA 120
           SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSY AFKFQTTAPKSCFMRPPGA
Sbjct: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYAAFKFQTTAPKSCFMRPPGA 120

Query: 121 ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV 180
           ILAPGESIIATVFKFVEVPE+NEKPVDQKNNRVKFKIMSLKVKG MDYVPELFDEQKDQV
Sbjct: 121 ILAPGESIIATVFKFVEVPENNEKPVDQKNNRVKFKIMSLKVKGPMDYVPELFDEQKDQV 180

Query: 181 SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE 240
           SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE
Sbjct: 181 SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE 240

Query: 241 RRERYLAKQQVEGVDSV 257
           RRERYLAKQQVEGVDSV
Sbjct: 241 RRERYLAKQQVEGVDSV 257

BLAST of CsGy6G033800 vs. ExPASy TrEMBL
Match: A0A6J1GCU7 (vesicle-associated protein 4-2-like OS=Cucurbita moschata OX=3662 GN=LOC111453036 PE=4 SV=1)

HSP 1 Score: 481 bits (1237), Expect = 7.09e-171
Identity = 245/257 (95.33%), Postives = 252/257 (98.05%), Query Frame = 0

Query: 1   MMAVEDQKSTSDGKVWGFLKQTFKHSTGGATTSSRTSHQTHPPVEGTNPHTSASVSSVAR 60
           MMA++DQKSTSDGKVWGFLKQTF+HS  GATTS RTSHQT+PPVE TNPHTSASVSSVAR
Sbjct: 1   MMAIDDQKSTSDGKVWGFLKQTFRHSASGATTSYRTSHQTNPPVERTNPHTSASVSSVAR 60

Query: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA 120
           SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA
Sbjct: 61  SLLPTRRRLKLDPSNKLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGA 120

Query: 121 ILAPGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV 180
           ILAPGES+IATVFKFVEVPE+NEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV
Sbjct: 121 ILAPGESLIATVFKFVEVPENNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQV 180

Query: 181 SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKE 240
           SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKP EE+GPKIIGEGLVIDEWKE
Sbjct: 181 SIEQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPAEETGPKIIGEGLVIDEWKE 240

Query: 241 RRERYLAKQQVEGVDSV 257
           RRERYLAKQQVEGVDSV
Sbjct: 241 RRERYLAKQQVEGVDSV 257

BLAST of CsGy6G033800 vs. TAIR 10
Match: AT4G21450.1 (PapD-like superfamily protein )

HSP 1 Score: 362.5 bits (929), Expect = 2.9e-100
Identity = 203/296 (68.58%), Postives = 227/296 (76.69%), Query Frame = 0

Query: 2   MAVEDQKSTSDGK-VWGFLKQTFKHSTG---------------GATTSSRTS-------- 61
           M + ++K TSDGK  WGF K  F++S+G               GA++SS +S        
Sbjct: 1   MTMTEEKPTSDGKGGWGFFKIPFRNSSGHRNAASSAATSPFPSGASSSSTSSHLHNHHQH 60

Query: 62  -----HQTHPPVEGTNPH-----------TSASVSSVARSLLPTRRRLKLDPSNKLFFPY 121
                HQ H  +    PH            S SVSSVA+S LPT+RRLKLDPS KL+FPY
Sbjct: 61  HHQHHHQHHHQLGYNGPHGDGSGQNQHPTPSPSVSSVAKSFLPTKRRLKLDPSEKLYFPY 120

Query: 122 EPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGAILAPGESIIATVFKFVEVPES 181
           EPGKQVRSAI IKNTSKS+VAFKFQTTAPKSCFMRPPGAILAPGE+IIATVFKFVE PE+
Sbjct: 121 EPGKQVRSAIKIKNTSKSHVAFKFQTTAPKSCFMRPPGAILAPGETIIATVFKFVEPPEN 180

Query: 182 NEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQVSIEQILRVVFLDPERPNAALE 241
           NEKP+DQ+ +RVKFKIMSLKVKG MDYVPELFDEQKD VS EQILRV+FLDPER N ALE
Sbjct: 181 NEKPMDQR-SRVKFKIMSLKVKGPMDYVPELFDEQKDDVSKEQILRVIFLDPERSNPALE 240

Query: 242 KLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKERRERYLAKQQVEGVDSV 258
           KLKRQLAEADAA+EARKKPPEE+GPK+IGEGLVIDEWKERRERYLA+QQ EG DSV
Sbjct: 241 KLKRQLAEADAAVEARKKPPEETGPKMIGEGLVIDEWKERRERYLAQQQGEGADSV 295

BLAST of CsGy6G033800 vs. TAIR 10
Match: AT4G21450.3 (PapD-like superfamily protein )

HSP 1 Score: 349.0 bits (894), Expect = 3.3e-96
Identity = 202/315 (64.13%), Postives = 227/315 (72.06%), Query Frame = 0

Query: 2   MAVEDQKSTSDGK-VWGFLKQTFKHSTG---------------GATTSSRTS-------- 61
           M + ++K TSDGK  WGF K  F++S+G               GA++SS +S        
Sbjct: 1   MTMTEEKPTSDGKGGWGFFKIPFRNSSGHRNAASSAATSPFPSGASSSSTSSHLHNHHQH 60

Query: 62  -----HQTHPPVEGTNPH-----------TSASVSSVARSLLPTRRRLKLDPSNKLFFPY 121
                HQ H  +    PH            S SVSSVA+S LPT+RRLKLDPS KL+FP+
Sbjct: 61  HHQHHHQHHHQLGYNGPHGDGSGQNQHPTPSPSVSSVAKSFLPTKRRLKLDPSEKLYFPW 120

Query: 122 -------------------EPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGAIL 181
                              EPGKQVRSAI IKNTSKS+VAFKFQTTAPKSCFMRPPGAIL
Sbjct: 121 FVSEDLRSIDTSLKFLSFNEPGKQVRSAIKIKNTSKSHVAFKFQTTAPKSCFMRPPGAIL 180

Query: 182 APGESIIATVFKFVEVPESNEKPVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQVSI 241
           APGE+IIATVFKFVE PE+NEKP+DQ+ +RVKFKIMSLKVKG MDYVPELFDEQKD VS 
Sbjct: 181 APGETIIATVFKFVEPPENNEKPMDQR-SRVKFKIMSLKVKGPMDYVPELFDEQKDDVSK 240

Query: 242 EQILRVVFLDPERPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKERR 258
           EQILRV+FLDPER N ALEKLKRQLAEADAA+EARKKPPEE+GPK+IGEGLVIDEWKERR
Sbjct: 241 EQILRVIFLDPERSNPALEKLKRQLAEADAAVEARKKPPEETGPKMIGEGLVIDEWKERR 300

BLAST of CsGy6G033800 vs. TAIR 10
Match: AT4G05060.1 (PapD-like superfamily protein )

HSP 1 Score: 316.6 bits (810), Expect = 1.8e-86
Identity = 186/288 (64.58%), Postives = 213/288 (73.96%), Query Frame = 0

Query: 2   MAVEDQKSTSDGKVWGFLKQTFKHSTGGATTSSRTS-------------------HQTH- 61
           MA+ + KS SDG+ WG  K  F++S   A ++S +S                   HQ+  
Sbjct: 1   MALTEDKSDSDGRRWGKFKLPFRNSNSQAPSASSSSSMATSSSSVTSSHLNQNYIHQSRH 60

Query: 62  -----PPVE---GTNPHTSA----SVSSVARSLLPTRRRLKLDPSNKLFFPYEPGKQVRS 121
                PPV    G N H SA    S+SSVARSLLPT+RRLKLDPS KL+FPYEPGKQVRS
Sbjct: 61  FQYHGPPVVEGLGQNHHQSAATIPSMSSVARSLLPTKRRLKLDPSAKLYFPYEPGKQVRS 120

Query: 122 AIGIKNTSKSYVAFKFQTTAPKSCFMRPPGAILAPGESIIATVFKFVEVPESNEKPVDQK 181
           AI IKNTSKS+VAFKFQTT PKSCFMRP GAILAPGE IIATVFKFVE PE+NEKP++QK
Sbjct: 121 AIKIKNTSKSHVAFKFQTTVPKSCFMRPAGAILAPGEEIIATVFKFVEPPENNEKPMEQK 180

Query: 182 NNRVKFKIMSLKVKGQMDYVPELFDEQKDQVSIEQILRVVFLDPERPNAALEKLKRQLAE 241
           +  VKFKIMSLK+K   DY+PELF+EQKD VS EQ++RVVFLDPE PN+ +EKLK QLAE
Sbjct: 181 SG-VKFKIMSLKMKVPTDYMPELFEEQKDHVSEEQVMRVVFLDPENPNSMMEKLKSQLAE 240

Query: 242 ADAALEARKKPPEE-SGPKIIGEGLVIDEWKERRERYLAKQQVEGVDS 257
           ADAA EARKK  E   GPK IGEGLVIDEWK+RRERYLA+QQ  GVD+
Sbjct: 241 ADAADEARKKASEGIVGPKPIGEGLVIDEWKQRRERYLAQQQ-GGVDA 286

BLAST of CsGy6G033800 vs. TAIR 10
Match: AT5G54110.1 (membrane-associated mannitol-induced )

HSP 1 Score: 315.5 bits (807), Expect = 4.1e-86
Identity = 163/243 (67.08%), Postives = 198/243 (81.48%), Query Frame = 0

Query: 16  WGFLKQTFKHSTGGATTSSRTSHQTHPPVEGTNPHTSASVSSVARSLLPTRRRLKLDPSN 75
           W     T   ST     + R+ H               ++SSVARSLLP RRRL+LDPS+
Sbjct: 24  WQRRSTTSSSSTQNPNQNYRSRHGNRNTDISAVSKPPLTMSSVARSLLPARRRLRLDPSS 83

Query: 76  KLFFPYEPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGAILAPGESIIATVFKF 135
            L+FPYEPGKQVRSAI +KNTSKS+ AFKFQTTAPKSC+MRPPG +LAPGES+ ATVFKF
Sbjct: 84  YLYFPYEPGKQVRSAIKLKNTSKSHTAFKFQTTAPKSCYMRPPGGVLAPGESVFATVFKF 143

Query: 136 VEVPESNEK-PVDQKNNRVKFKIMSLKVKGQMDYVPELFDEQKDQVSIEQILRVVFLDPE 195
           VE PE+NEK P++QK ++VKFKIMSLKVK  ++YVPELFDEQKDQV++EQ+LRV+F+D +
Sbjct: 144 VEHPENNEKQPLNQK-SKVKFKIMSLKVKPGVEYVPELFDEQKDQVAVEQVLRVIFIDAD 203

Query: 196 RPNAALEKLKRQLAEADAALEARKKPPEESGPKIIGEGLVIDEWKERRERYLAKQQVEGV 255
           RP+AALEKLKRQL EA+AA+EARKKPP E+GP+++GEGLVIDEWKERRE+YLA+QQVE V
Sbjct: 204 RPSAALEKLKRQLDEAEAAVEARKKPPPETGPRVVGEGLVIDEWKERREKYLARQQVESV 263

Query: 256 DSV 258
           DS+
Sbjct: 264 DSL 265

BLAST of CsGy6G033800 vs. TAIR 10
Match: AT4G21450.2 (PapD-like superfamily protein )

HSP 1 Score: 220.7 bits (561), Expect = 1.4e-57
Identity = 129/211 (61.14%), Postives = 148/211 (70.14%), Query Frame = 0

Query: 2   MAVEDQKSTSDGK-VWGFLKQTFKHSTG---------------GATTSSRTS-------- 61
           M + ++K TSDGK  WGF K  F++S+G               GA++SS +S        
Sbjct: 1   MTMTEEKPTSDGKGGWGFFKIPFRNSSGHRNAASSAATSPFPSGASSSSTSSHLHNHHQH 60

Query: 62  -----HQTHPPVEGTNPH-----------TSASVSSVARSLLPTRRRLKLDPSNKLFFPY 121
                HQ H  +    PH            S SVSSVA+S LPT+RRLKLDPS KL+FPY
Sbjct: 61  HHQHHHQHHHQLGYNGPHGDGSGQNQHPTPSPSVSSVAKSFLPTKRRLKLDPSEKLYFPY 120

Query: 122 EPGKQVRSAIGIKNTSKSYVAFKFQTTAPKSCFMRPPGAILAPGESIIATVFKFVEVPES 173
           EPGKQVRSAI IKNTSKS+VAFKFQTTAPKSCFMRPPGAILAPGE+IIATVFKFVE PE+
Sbjct: 121 EPGKQVRSAIKIKNTSKSHVAFKFQTTAPKSCFMRPPGAILAPGETIIATVFKFVEPPEN 180

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VYN24.1e-9968.58Vesicle-associated protein 4-2 OS=Arabidopsis thaliana OX=3702 GN=PVA42 PE=1 SV=... [more]
Q8LPQ72.6e-8564.58Vesicle-associated protein 4-3 OS=Arabidopsis thaliana OX=3702 GN=PVA43 PE=2 SV=... [more]
Q1ECE05.7e-8567.08Vesicle-associated protein 4-1 OS=Arabidopsis thaliana OX=3702 GN=PVA41 PE=2 SV=... [more]
Q9SHC83.4e-0530.00Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=... [more]
Q9SYC92.2e-0426.77Vesicle-associated protein 1-4 OS=Arabidopsis thaliana OX=3702 GN=PVA14 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
XP_004134732.26.05e-179100.00vesicle-associated protein 4-2 [Cucumis sativus] >KGN49181.1 hypothetical protei... [more]
XP_008439916.18.24e-17798.83PREDICTED: vesicle-associated protein 4-2 [Cucumis melo][more]
XP_038883586.11.31e-17396.89vesicle-associated protein 4-2 [Benincasa hispida][more]
XP_022977981.11.26e-17195.72vesicle-associated protein 4-2-like [Cucurbita maxima] >XP_023543767.1 vesicle-a... [more]
XP_022142290.17.26e-17195.72vesicle-associated protein 4-2 [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A0A0KN702.93e-179100.00MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G516890 PE=4 SV... [more]
A0A1S3AZX43.99e-17798.83vesicle-associated protein 4-2 OS=Cucumis melo OX=3656 GN=LOC103484561 PE=4 SV=1[more]
A0A6J1ILI26.08e-17295.72vesicle-associated protein 4-2-like OS=Cucurbita maxima OX=3661 GN=LOC111478109 ... [more]
A0A6J1CKI93.51e-17195.72vesicle-associated protein 4-2 OS=Momordica charantia OX=3673 GN=LOC111012445 PE... [more]
A0A6J1GCU77.09e-17195.33vesicle-associated protein 4-2-like OS=Cucurbita moschata OX=3662 GN=LOC11145303... [more]
Match NameE-valueIdentityDescription
AT4G21450.12.9e-10068.58PapD-like superfamily protein [more]
AT4G21450.33.3e-9664.13PapD-like superfamily protein [more]
AT4G05060.11.8e-8664.58PapD-like superfamily protein [more]
AT5G54110.14.1e-8667.08membrane-associated mannitol-induced [more]
AT4G21450.21.4e-5761.14PapD-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 197..217
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 26..60
NoneNo IPR availablePANTHERPTHR10809:SF101VESICLE-ASSOCIATED MEMBRANE-PROTEIN-ASSOCIATED PROTEIN-RELATEDcoord: 35..256
IPR000535Major sperm protein (MSP) domainPFAMPF00635Motile_Spermcoord: 69..180
e-value: 2.0E-28
score: 98.4
IPR000535Major sperm protein (MSP) domainPROSITEPS50202MSPcoord: 68..191
score: 20.963341
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 64..195
e-value: 1.8E-26
score: 94.5
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 35..256
IPR008962PapD-like superfamilySUPERFAMILY49354PapD-likecoord: 57..201

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy6G033800.1CsGy6G033800.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090158 endoplasmic reticulum membrane organization
biological_process GO:0061817 endoplasmic reticulum-plasma membrane tethering
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0005886 plasma membrane