CsGy6G033260 (gene) Cucumber (Gy14) v2.1

BLAST of CsGy6G033260 vs. ExPASy Swiss-Prot
Match: P83947 (Pectinesterase/pectinesterase inhibitor OS=Ficus pumila var. awkeotsang OX=204231 PE=1 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 2.5e-170
Identity = 311/559 (55.64%), Postives = 393/559 (70.30%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           M  +QP  LL+  K+  SK    I  F  V+  A  ++  F  P     +L     IC +
Sbjct: 1   MEINQP-NLLEASKSCYSK----ITFFLLVISFAALVSTGFSSP-----ELSLHHKICDQ 60

Query: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPRGE 120
           + +  SC+ M +    L++    N    L+SFL K+ P++    E A D  +RIN+P+  
Sbjct: 61  SVNKESCLAMISEVTGLNMADHRN---LLKSFLEKTTPRIQKAFETANDASRRINNPQER 120

Query: 121 AALADCIELMEISNGRIMDSVLAL--KNRTNGSIENSHTWLSSVLTNHVTCWDEVESSLS 180
            AL DC ELM++S  R++DS+  L  +N T  S E+ H WLS VLTNHVTC D +E   +
Sbjct: 121 TALLDCAELMDLSKERVVDSISILFHQNLTTRSHEDLHVWLSGVLTNHVTCLDGLEEGST 180

Query: 181 R--AAAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGV 240
                 M+  L ELI+R R SL + V+++         +E     +P+W+  GDRRLL  
Sbjct: 181 DYIKTLMESHLNELILRARTSLAIFVTLFP---AKSNVIEPVTGNFPTWVTAGDRRLLQT 240

Query: 241 LGREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVM 300
           LG+++EP+IVVAKDGSG+++T+ EAV ++PD SK R+++ V+ G YEENV+ G +KKNVM
Sbjct: 241 LGKDIEPDIVVAKDGSGDYETLNEAVAAIPDNSKKRVIVLVRTGIYEENVDFGYQKKNVM 300

Query: 301 IVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVG 360
           +VG+GMD TIITGS NVVDGSTTF SATVAAVGDGFIAQDI FQNTAGPEK+QAVALR+G
Sbjct: 301 LVGEGMDYTIITGSRNVVDGSTTFDSATVAAVGDGFIAQDICFQNTAGPEKYQAVALRIG 360

Query: 361 ADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNN 420
           AD++VINRCRIDAYQDTLY H+ RQFYRD  ITGTVDFIFGNAAVV QNC + PR+ M  
Sbjct: 361 ADETVINRCRIDAYQDTLYPHNYRQFYRDRNITGTVDFIFGNAAVVFQNCNLIPRKQMKG 420

Query: 421 QANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRI 480
           Q N +TAQGR DPNQNTGTSIQ C+I AS+DLEPV+ + K+YLGRPWKEYSRTV+M+S I
Sbjct: 421 QENTITAQGRTDPNQNTGTSIQNCEIFASADLEPVEDTFKSYLGRPWKEYSRTVVMESYI 480

Query: 481 GDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDS 540
            D+I PAGW EW+ DFALKTL+YGEY N GPGS  S+RVKW GYH+ITSP  A++FTV  
Sbjct: 481 SDVIDPAGWLEWDRDFALKTLFYGEYRNGGPGSGTSERVKWPGYHVITSPEVAEQFTVAE 540

Query: 541 LIQGGEWLGPSGATFIPGL 556
           LIQGG WLG +G  +  GL
Sbjct: 541 LIQGGSWLGSTGVDYTAGL 543

BLAST of CsGy6G033260 vs. ExPASy Swiss-Prot
Match: Q1JPL7 (Pectinesterase/pectinesterase inhibitor 18 OS=Arabidopsis thaliana OX=3702 GN=PME18 PE=1 SV=3)

HSP 1 Score: 579.3 bits (1492), Expect = 4.6e-164
Identity = 309/558 (55.38%), Postives = 387/558 (69.35%), Query Frame = 0

Query: 3   THQPLLLLQPPKTSRSKTIPTILCFAAVLCSAIFITNKFIKPNPSENDLPFL--QNICHK 62
           ++QP  LL  PK+ + K +  +L F A+L S  F T + I  N + ND   L    ICH 
Sbjct: 4   SNQP--LLSKPKSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLLTTSQICHG 63

Query: 63  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPRGE 122
           A+D  SC +   SEF    +   N +D L  FL+ S+ ++ ST+    + R R N  R +
Sbjct: 64  AHDQDSC-QALLSEFTTLSLSKLNRLDLLHVFLKNSVWRLESTMTMVSEARIRSNGVRDK 123

Query: 123 AALADCIELMEISNGRIMDSVLALK--NRTNGSIENSHTWLSSVLTNHVTCWDEV-ESSL 182
           A  ADC E+M++S  R+M S+  L+  N    S  N HTWLSSVLTN++TC + + + S+
Sbjct: 124 AGFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYMTCLESISDVSV 183

Query: 183 SRAAAMDLGLEELIMRGRNSLGMLVSIWGL--DIKNLGELEKKGNGYPSWLKKGDRRLLG 242
           +    +   LE+L+ R R +L + VS+     D+K +       N +PSWL   DR+LL 
Sbjct: 184 NSKQIVKPQLEDLVSRARVALAIFVSVLPARDDLKMI-----ISNRFPSWLTALDRKLLE 243

Query: 243 VLGREME--PNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKK 302
              + ++   N+VVAKDG+G FKTV EAV + P+ S  R VIYVK+G Y+E +++GKKKK
Sbjct: 244 SSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYVIYVKKGVYKETIDIGKKKK 303

Query: 303 NVMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVAL 362
           N+M+VGDG D+TIITGSLNV+DGSTTF+SATVAA GDGF+AQDIWFQNTAGP KHQAVAL
Sbjct: 304 NLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQDIWFQNTAGPAKHQAVAL 363

Query: 363 RVGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRP 422
           RV ADQ+VINRCRIDAYQDTLYTH+ RQFYRDS ITGTVDFIFGN+AVV QNC I  R P
Sbjct: 364 RVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNSAVVFQNCDIVARNP 423

Query: 423 MNNQANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQ 482
              Q NM+TAQGR D NQNT  SIQ+C I ASSDL PVK S+KT+LGRPWK YSRTVIMQ
Sbjct: 424 GAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFLGRPWKLYSRTVIMQ 483

Query: 483 SRIGDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFT 542
           S I + I PAGW  W+G+FAL TLYYGEY+NTGPG+D SKRV W G+ +I    EA++FT
Sbjct: 484 SFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKRVNWKGFKVIKDSKEAEQFT 543

Query: 543 VDSLIQGGEWLGPSGATF 552
           V  LIQGG WL P+G TF
Sbjct: 544 VAKLIQGGLWLKPTGVTF 553

BLAST of CsGy6G033260 vs. ExPASy Swiss-Prot
Match: P09607 (Pectinesterase 2.1 OS=Solanum lycopersicum OX=4081 GN=PME2.1 PE=2 SV=2)

HSP 1 Score: 571.2 bits (1471), Expect = 1.2e-161
Identity = 302/559 (54.03%), Postives = 384/559 (68.69%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPT--ILCFAAVLCSAIFITNKFIKPNPSENDLPFLQNIC 60
           MAT Q  LL    KT +  +I +  IL F   L  A+F+    + P   E       N+C
Sbjct: 1   MATPQQPLL---TKTHKQNSIISFKILTFVVTLFVALFLVVFLVAPYQFEIK---HSNLC 60

Query: 61  HKAYDPSSCIEMAASEFPLSIIKTTNE-VDFLQSFLRKSMPKVISTIERAKDIRQRINSP 120
             A D   C+   +      I+ + ++ +  L+ FL  S+ ++ + I   + I+ +IN  
Sbjct: 61  KTAQDSQLCLSYVSDLISNEIVTSDSDGLSILKKFLVYSVHQMNNAIPVVRKIKNQINDI 120

Query: 121 RGEAALADCIELMEISNGRIMDSVLALKNRTNGSIENSHTWLSSVLTNHVTCWDEVESSL 180
           R + AL DC+EL+++S   + DS+ A+  R+     N+ +WLS VLTNHVTC DE++ S 
Sbjct: 121 REQGALTDCLELLDLSVDLVCDSIAAIDKRSRSEHANAQSWLSGVLTNHVTCLDELD-SF 180

Query: 181 SRAAAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNG-YPSWLKKGDRRLLGV 240
           ++A      L+ELI R + +L ML S+   +     E+ + G G  PSW+   DR+L+  
Sbjct: 181 TKAMINGTNLDELISRAKVALAMLASVTTPN----DEVLRPGLGKMPSWVSSRDRKLMES 240

Query: 241 LGREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVM 300
            G+++  N VVAKDG+G ++T+ EAV + PDKSK R VIYVKRGTY+ENVEV  +K N+M
Sbjct: 241 SGKDIGANAVVAKDGTGKYRTLAEAVAAAPDKSKTRYVIYVKRGTYKENVEVSSRKMNLM 300

Query: 301 IVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVG 360
           I+GDGM +TIITGSLNVVDGSTTF SAT+AAVG GFI QDI  QNTAGP KHQAVALRVG
Sbjct: 301 IIGDGMYATIITGSLNVVDGSTTFHSATLAAVGKGFILQDICIQNTAGPAKHQAVALRVG 360

Query: 361 ADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNN 420
           AD+SVINRCRIDAYQDTLY HS RQFYRDS +TGT+DFIFGNAAVV Q C++  R+P   
Sbjct: 361 ADKSVINRCRIDAYQDTLYAHSQRQFYRDSYVTGTIDFIFGNAAVVFQKCQLVARKPGKY 420

Query: 421 QANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRI 480
           Q NMVTAQGR DPNQ TGTSIQ CDI+AS DL+PV K   TYLGRPWK+YSRTV+M+S +
Sbjct: 421 QQNMVTAQGRTDPNQATGTSIQFCDIIASPDLKPVVKEFPTYLGRPWKKYSRTVVMESYL 480

Query: 481 GDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDS 540
           G LI P+GWAEW+GDFALKTLYYGE+ N GPG+  SKRVKW GYH+IT P+EA  FTV  
Sbjct: 481 GGLIDPSGWAEWHGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAEAMSFTVAK 540

Query: 541 LIQGGEWLGPSGATFIPGL 556
           LIQGG WL  +   ++ GL
Sbjct: 541 LIQGGSWLRSTDVAYVDGL 548

BLAST of CsGy6G033260 vs. ExPASy Swiss-Prot
Match: Q96575 (Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 2.8e-161
Identity = 304/558 (54.48%), Postives = 376/558 (67.38%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPT--ILCFAAVLCSAIFITNKFIKPNPSENDLPFLQNIC 60
           MAT Q  LL    KT +  +I +  IL F   L  A+F+    + P   E       N+C
Sbjct: 1   MATPQQPLL---TKTHKQNSIISFKILTFVVTLFVALFLVVFLVAPYQFEIK---HSNLC 60

Query: 61  HKAYDPSSCIEMAASEFPLSIIKTTNE-VDFLQSFLRKSMPKVISTIERAKDIRQRINSP 120
             A D   C+   +      I+ T ++ +  L  FL   + ++ + I     ++ +IN  
Sbjct: 61  KTAQDSQLCLSYVSDLMSNEIVTTDSDGLSILMKFLVNYVHQMNNAIPVVSKMKNQINDI 120

Query: 121 RGEAALADCIELMEISNGRIMDSVLALKNRTNGSIENSHTWLSSVLTNHVTCWDEVESSL 180
           R E AL DC+EL++ S   + DS+ A+  RT+    N+ +WLS VLTNHVTC DE++ S 
Sbjct: 121 RQEGALTDCLELLDQSVDLVSDSIAAIDKRTHSEHANAQSWLSGVLTNHVTCLDELD-SF 180

Query: 181 SRAAAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVL 240
           ++A      L+ELI R + +L ML S+      N   L       PSW+   DR+L+   
Sbjct: 181 TKAMINGTNLDELISRAKVALAMLASV---TTPNDDVLRPGLGKMPSWVSSRDRKLMESS 240

Query: 241 GREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMI 300
           G+++  N VVAKDG+G ++T+ EAV + PDKSK R VIYVKRG Y+ENVEV  +K  +MI
Sbjct: 241 GKDIGANAVVAKDGTGKYRTLAEAVAAAPDKSKTRYVIYVKRGIYKENVEVSSRKMKLMI 300

Query: 301 VGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGA 360
           VGDGM +TIITG+LNVVDGSTTF SAT+AAVG GFI QDI  QNTAGP KHQAVALRVGA
Sbjct: 301 VGDGMHATIITGNLNVVDGSTTFHSATLAAVGKGFILQDICIQNTAGPAKHQAVALRVGA 360

Query: 361 DQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQ 420
           D+SVINRCRIDAYQDTLY HS RQFYRDS +TGT+DFIFGNAAVV Q CK+  R+P   Q
Sbjct: 361 DKSVINRCRIDAYQDTLYAHSQRQFYRDSYVTGTIDFIFGNAAVVFQKCKLVARKPGKYQ 420

Query: 421 ANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIG 480
            NMVTAQGR DPNQ TGTSIQ C+I+ASSDLEPV K   TYLGRPWK+YSRTV+M+S +G
Sbjct: 421 QNMVTAQGRTDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKKYSRTVVMESYLG 480

Query: 481 DLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSL 540
            LI PAGWAEW+GDFALKTLYYGE+ N GPG+  SKRVKW GYH IT P+EA  FTV  L
Sbjct: 481 GLINPAGWAEWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHCITDPAEAMPFTVAKL 540

Query: 541 IQGGEWLGPSGATFIPGL 556
           IQGG WL  +G  ++ GL
Sbjct: 541 IQGGSWLRSTGVAYVDGL 548

BLAST of CsGy6G033260 vs. ExPASy Swiss-Prot
Match: P14280 (Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5)

HSP 1 Score: 558.9 bits (1439), Expect = 6.4e-158
Identity = 301/558 (53.94%), Postives = 377/558 (67.56%), Query Frame = 0

Query: 5   QPLLLLQPPKTSRSKTIPT--ILCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHKAY 64
           QPLL+    KT +   I +  IL F   L  A+F+        P + ++    N+C  A 
Sbjct: 6   QPLLI----KTHKQNPIISFKILSFVITLFVALFLV------APYQVEIKH-SNLCKTAQ 65

Query: 65  DPSSCIEMAASEFPLSIIKTTNE-VDFLQSFLRKSMPKVISTIERAKDIRQRINSPRGEA 124
           D   C+   +      I+ T ++    L  FL   + ++ + I   + ++ +IN  R   
Sbjct: 66  DSQLCLSYVSDLISNEIVTTESDGHSILMKFLVNYVHQMNNAIPVVRKMKNQINDIRQHG 125

Query: 125 ALADCIELMEISNGRIMDSVLALKNRTNGSIENSHTWLSSVLTNHVTCWDEVESSLSRAA 184
           AL DC+EL++ S     DS+ A+  R+     N+ +WLS VLTNHVTC DE++ S ++A 
Sbjct: 126 ALTDCLELLDQSVDFASDSIAAIDKRSRSEHANAQSWLSGVLTNHVTCLDELD-SFTKAM 185

Query: 185 AMDLGLEELIMRGRNSLGMLVSIWGLD----IKNLGELEKKGNGYPSWLKKGDRRLLGVL 244
                LEELI R + +L ML S+   D    +  LG++       PSW+   DR+L+   
Sbjct: 186 INGTNLEELISRAKVALAMLASLTTQDEDVFMTVLGKM-------PSWVSSMDRKLMESS 245

Query: 245 GREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMI 304
           G+++  N VVA+DG+G+++T+ EAV + PDKSK R VIYVKRGTY+ENVEV   K N+MI
Sbjct: 246 GKDIIANAVVAQDGTGDYQTLAEAVAAAPDKSKTRYVIYVKRGTYKENVEVASNKMNLMI 305

Query: 305 VGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGA 364
           VGDGM +T ITGSLNVVDGSTTF+SAT+AAVG GFI QDI  QNTAGP K QAVALRVGA
Sbjct: 306 VGDGMYATTITGSLNVVDGSTTFRSATLAAVGQGFILQDICIQNTAGPAKDQAVALRVGA 365

Query: 365 DQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQ 424
           D SVINRCRIDAYQDTLY HS RQFYRDS +TGTVDFIFGNAAVV Q C++  R+P   Q
Sbjct: 366 DMSVINRCRIDAYQDTLYAHSQRQFYRDSYVTGTVDFIFGNAAVVFQKCQLVARKPGKYQ 425

Query: 425 ANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIG 484
            NMVTAQGR DPNQ TGTSIQ C+I+ASSDLEPV K   TYLGRPWKEYSRTV+M+S +G
Sbjct: 426 QNMVTAQGRTDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKEYSRTVVMESYLG 485

Query: 485 DLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSL 544
            LI PAGWAEW+GDFALKTLYYGE+ N GPG+  SKRVKW GYH+IT P++A  FTV  L
Sbjct: 486 GLINPAGWAEWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAKAMPFTVAKL 544

Query: 545 IQGGEWLGPSGATFIPGL 556
           IQGG WL  +G  ++ GL
Sbjct: 546 IQGGSWLRSTGVAYVDGL 544

BLAST of CsGy6G033260 vs. NCBI nr
Match: XP_004134752.1 (pectinesterase [Cucumis sativus] >KGN49128.1 hypothetical protein Csa_004290 [Cucumis sativus])

HSP 1 Score: 1107 bits (2864), Expect = 0.0
Identity = 552/555 (99.46%), Postives = 554/555 (99.82%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLLLLQPPKTSRSKTIPT+LCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK
Sbjct: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60

Query: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPRGE 120
           AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPRGE
Sbjct: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPRGE 120

Query: 121 AALADCIELMEISNGRIMDSVLALKNRTNGSIENSHTWLSSVLTNHVTCWDEVESSLSRA 180
           AALADCIELMEISNGRIMDSVLALKNRT+GSIENSHTWLSSVLTNHVTCWDEVESSLSRA
Sbjct: 121 AALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLSRA 180

Query: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240
           A MDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE
Sbjct: 181 APMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240

Query: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300
           MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD
Sbjct: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI 480

Query: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLIQG 540
           QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLIQG
Sbjct: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLIQG 540

Query: 541 GEWLGPSGATFIPGL 555
           GEWLGPSGATFIPGL
Sbjct: 541 GEWLGPSGATFIPGL 555

BLAST of CsGy6G033260 vs. NCBI nr
Match: TYK13062.1 (pectinesterase [Cucumis melo var. makuwa])

HSP 1 Score: 1051 bits (2718), Expect = 0.0
Identity = 522/555 (94.05%), Postives = 541/555 (97.48%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLL LQPPKTS SKTI TILCFA VLCSAIF+TNKFIK NP ENDLPFL NICHK
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYENDLPFLHNICHK 60

Query: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPRGE 120
           AY+PSSCIEMAASEFPLSIIKTTNE  FLQSFL+KSMPKVI+TIERAKD+RQRINSPRGE
Sbjct: 61  AYNPSSCIEMAASEFPLSIIKTTNEEHFLQSFLKKSMPKVINTIERAKDLRQRINSPRGE 120

Query: 121 AALADCIELMEISNGRIMDSVLALKNRTNGSIENSHTWLSSVLTNHVTCWDEVESSLSRA 180
            AL DCIELMEISNGRIMDSVLALKNRT+GSIE+SHTWLSSVLTNHVTCWDEVE+SLSRA
Sbjct: 121 GALDDCIELMEISNGRIMDSVLALKNRTSGSIESSHTWLSSVLTNHVTCWDEVETSLSRA 180

Query: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240
           AAMDLGLEELIMRGRNSLGMLVSIWGL+IKNLGEL K+GNGYPSWLKKGDRRLLGVLGRE
Sbjct: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGNGYPSWLKKGDRRLLGVLGRE 240

Query: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300
           MEPNIVVAKDGSGNFKTV+EAVESVPDKSK+RIVIYVK+GTY+ENVEVGKKKKNVMIVGD
Sbjct: 241 MEPNIVVAKDGSGNFKTVQEAVESVPDKSKDRIVIYVKKGTYKENVEVGKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGP KHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTV+MQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI 480

Query: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLIQG 540
           QPAGWAEW+GDFALKTLYYGEYSNTGPGSDVSKRVKW GYH+ITSPSEAQKFTV+SLIQG
Sbjct: 481 QPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTVNSLIQG 540

Query: 541 GEWLGPSGATFIPGL 555
           GEWLGPSGA+FIPGL
Sbjct: 541 GEWLGPSGASFIPGL 555

BLAST of CsGy6G033260 vs. NCBI nr
Match: XP_008439965.1 (PREDICTED: pectinesterase [Cucumis melo])

HSP 1 Score: 1050 bits (2714), Expect = 0.0
Identity = 522/555 (94.05%), Postives = 539/555 (97.12%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLL LQPPKTS SKTI TILCFA VLCSAIF+TNKFIK NP ENDLPFL NICHK
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYENDLPFLHNICHK 60

Query: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPRGE 120
           AY+PSSCIEMAASEFPLSIIKTTNE  FLQSFL+KSMPKVI+TIERAKD+RQRINSPRGE
Sbjct: 61  AYNPSSCIEMAASEFPLSIIKTTNEEHFLQSFLKKSMPKVINTIERAKDLRQRINSPRGE 120

Query: 121 AALADCIELMEISNGRIMDSVLALKNRTNGSIENSHTWLSSVLTNHVTCWDEVESSLSRA 180
            AL DCIELMEISNGRIMDSVLALKNRT+G IE+SHTWLSSVLTNHVTCWDEVESSLSRA
Sbjct: 121 GALDDCIELMEISNGRIMDSVLALKNRTSGYIESSHTWLSSVLTNHVTCWDEVESSLSRA 180

Query: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240
           AAMDLGLEELIMRGRNSLGMLVSIWGL+IKNLGEL K+GNGYPSWLKKGDRRLLGVLGRE
Sbjct: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGNGYPSWLKKGDRRLLGVLGRE 240

Query: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300
           MEPNIVVAKDGSGNFKTV+EAVESVPDKSKNRIVIYVK+GTY+ENVEVGKKKKNVMIVGD
Sbjct: 241 MEPNIVVAKDGSGNFKTVQEAVESVPDKSKNRIVIYVKKGTYKENVEVGKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGP KHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIQQCDIVAS DLEPVKKSIKTYLGRPWKEYSRTV+MQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIQQCDIVASFDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI 480

Query: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLIQG 540
           QPAGWAEW+GDFALKTLYYGEYSNTGPGSDVSKRVKW GYH+ITSPSEAQKFTV+SLIQG
Sbjct: 481 QPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTVNSLIQG 540

Query: 541 GEWLGPSGATFIPGL 555
           GEWLGPSGA+FIPGL
Sbjct: 541 GEWLGPSGASFIPGL 555

BLAST of CsGy6G033260 vs. NCBI nr
Match: XP_038881670.1 (pectinesterase [Benincasa hispida])

HSP 1 Score: 1001 bits (2587), Expect = 0.0
Identity = 498/556 (89.57%), Postives = 527/556 (94.78%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLL  QPPKTSRSKTIPTILCFAAVLCSAIFITNKFIK N SENDL FL N+CHK
Sbjct: 1   MATHQPLL--QPPKTSRSKTIPTILCFAAVLCSAIFITNKFIKSNSSENDLSFLHNLCHK 60

Query: 61  AYDPSSCIEMAASEFPLSI-IKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPRG 120
           AY+PSSCIEMAASEFP    IKTTN   FLQSFLRKS+PKVI+TI+RAKD+ QRIN+PRG
Sbjct: 61  AYNPSSCIEMAASEFPFPREIKTTNRGHFLQSFLRKSIPKVINTIDRAKDLSQRINNPRG 120

Query: 121 EAALADCIELMEISNGRIMDSVLALKNRTNGSIENSHTWLSSVLTNHVTCWDEVESSLSR 180
           EAALADC+ELMEISN RI+DSV ALKNRT+GSIEN+HTWLSSVLTNH+TCWDEVE+S+SR
Sbjct: 121 EAALADCVELMEISNSRIIDSVSALKNRTSGSIENAHTWLSSVLTNHITCWDEVENSVSR 180

Query: 181 AAAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGR 240
           AAAMDLGLEELIMRGRNSLGM VSIW  +I+N+GELEK+ NGYPSWLKKGDRRLLGV G+
Sbjct: 181 AAAMDLGLEELIMRGRNSLGMFVSIWESEIENMGELEKRVNGYPSWLKKGDRRLLGVPGK 240

Query: 241 EMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVG 300
           EME NIVVAKDGSGNF TV EAVESVPDKSKNRIVIYVK+G Y+E VEVGKKKKNVMIVG
Sbjct: 241 EMEANIVVAKDGSGNFMTVTEAVESVPDKSKNRIVIYVKKGIYKEKVEVGKKKKNVMIVG 300

Query: 301 DGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQ 360
           DGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQ
Sbjct: 301 DGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQ 360

Query: 361 SVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQAN 420
           SVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM+NQAN
Sbjct: 361 SVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQAN 420

Query: 421 MVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDL 480
           MVTAQGR+DPNQNTG+SIQ+C+IVASSDLEPVK+SIKTYLGRPWKEYSRTV+MQS IGDL
Sbjct: 421 MVTAQGRMDPNQNTGSSIQKCEIVASSDLEPVKQSIKTYLGRPWKEYSRTVVMQSTIGDL 480

Query: 481 IQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLIQ 540
           IQPAGWAEWNGDFALKTLYYGEYSN GPGSDVSKRVKW GYH+ITSPSEAQKFTVDSLIQ
Sbjct: 481 IQPAGWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWAGYHVITSPSEAQKFTVDSLIQ 540

Query: 541 GGEWLGPSGATFIPGL 555
           GGEWLGPSGATFIPGL
Sbjct: 541 GGEWLGPSGATFIPGL 554

BLAST of CsGy6G033260 vs. NCBI nr
Match: XP_023002934.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 886 bits (2289), Expect = 0.0
Identity = 448/557 (80.43%), Postives = 495/557 (88.87%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAAVLCSAIFITNKFIKPNPSENDL-PFLQNICH 60
           MATH+PLL  QPP+T+R KT  TIL F+ +L SAIFITNK I  NPSEND  PFL NIC+
Sbjct: 1   MATHEPLLP-QPPETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICY 60

Query: 61  KAYDPSSCIEMAASEFPL-SIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPR 120
           KAY+ SSCI+M ASEFPL    KTTN  +FLQSFLRKS  K+++TIE AKD+R+RIN+PR
Sbjct: 61  KAYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPR 120

Query: 121 GEAALADCIELMEISNGRIMDSVLALKNRTNGSIENSHTWLSSVLTNHVTCWDEVESSLS 180
           GEAALADC+ELMEIS  RI DS+LAL+ R  G+IEN+HTWLSSVLTNHV C DE+E+S+S
Sbjct: 121 GEAALADCVELMEISMDRITDSILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSIS 180

Query: 181 RAAAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLG 240
           +AAAMDLG+EELI+ GRNSLGMLVSIWG         +K+G GYPSWLKKGDRRLL V+G
Sbjct: 181 KAAAMDLGVEELIVIGRNSLGMLVSIWGSG-------KKEGMGYPSWLKKGDRRLLEVVG 240

Query: 241 REMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIV 300
           R M+ +IVVAKDGSGNFKTVKEAVE+VPDKSK+RIVIYVK+G Y+ENVEVGK KKNVM+V
Sbjct: 241 RGMKADIVVAKDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLV 300

Query: 301 GDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGAD 360
           GDGMDSTIIT SLNVVDGSTTFKSATVAAVGDGFIAQDIWF+NTA PEKHQAVALRVGAD
Sbjct: 301 GDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGAD 360

Query: 361 QSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQA 420
           QSVINRCRIDAYQDTLY H+NRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM+NQA
Sbjct: 361 QSVINRCRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQA 420

Query: 421 NMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGD 480
           NMVTAQGR DPNQNTGTSIQ+C+IVAS DLEPVKKSIKTYLGRPWKEYSRTV+MQS +GD
Sbjct: 421 NMVTAQGRTDPNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGD 480

Query: 481 LIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLI 540
           LI PAGWAEWNG+FALKTLYYGEYSN GPGS+VSKRVKW GYH+ITSP+ AQKFTVDSLI
Sbjct: 481 LIDPAGWAEWNGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLI 540

Query: 541 QGGEWLGPSGATFIPGL 555
           QG EWLG SG TFIPGL
Sbjct: 541 QGAEWLGSSGVTFIPGL 549

BLAST of CsGy6G033260 vs. ExPASy TrEMBL
Match: A0A0A0KHV5 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G514890 PE=3 SV=1)

HSP 1 Score: 1107 bits (2864), Expect = 0.0
Identity = 552/555 (99.46%), Postives = 554/555 (99.82%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLLLLQPPKTSRSKTIPT+LCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK
Sbjct: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60

Query: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPRGE 120
           AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPRGE
Sbjct: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPRGE 120

Query: 121 AALADCIELMEISNGRIMDSVLALKNRTNGSIENSHTWLSSVLTNHVTCWDEVESSLSRA 180
           AALADCIELMEISNGRIMDSVLALKNRT+GSIENSHTWLSSVLTNHVTCWDEVESSLSRA
Sbjct: 121 AALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLSRA 180

Query: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240
           A MDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE
Sbjct: 181 APMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240

Query: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300
           MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD
Sbjct: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI 480

Query: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLIQG 540
           QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLIQG
Sbjct: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLIQG 540

Query: 541 GEWLGPSGATFIPGL 555
           GEWLGPSGATFIPGL
Sbjct: 541 GEWLGPSGATFIPGL 555

BLAST of CsGy6G033260 vs. ExPASy TrEMBL
Match: A0A5D3CNY5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006640 PE=3 SV=1)

HSP 1 Score: 1051 bits (2718), Expect = 0.0
Identity = 522/555 (94.05%), Postives = 541/555 (97.48%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLL LQPPKTS SKTI TILCFA VLCSAIF+TNKFIK NP ENDLPFL NICHK
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYENDLPFLHNICHK 60

Query: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPRGE 120
           AY+PSSCIEMAASEFPLSIIKTTNE  FLQSFL+KSMPKVI+TIERAKD+RQRINSPRGE
Sbjct: 61  AYNPSSCIEMAASEFPLSIIKTTNEEHFLQSFLKKSMPKVINTIERAKDLRQRINSPRGE 120

Query: 121 AALADCIELMEISNGRIMDSVLALKNRTNGSIENSHTWLSSVLTNHVTCWDEVESSLSRA 180
            AL DCIELMEISNGRIMDSVLALKNRT+GSIE+SHTWLSSVLTNHVTCWDEVE+SLSRA
Sbjct: 121 GALDDCIELMEISNGRIMDSVLALKNRTSGSIESSHTWLSSVLTNHVTCWDEVETSLSRA 180

Query: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240
           AAMDLGLEELIMRGRNSLGMLVSIWGL+IKNLGEL K+GNGYPSWLKKGDRRLLGVLGRE
Sbjct: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGNGYPSWLKKGDRRLLGVLGRE 240

Query: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300
           MEPNIVVAKDGSGNFKTV+EAVESVPDKSK+RIVIYVK+GTY+ENVEVGKKKKNVMIVGD
Sbjct: 241 MEPNIVVAKDGSGNFKTVQEAVESVPDKSKDRIVIYVKKGTYKENVEVGKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGP KHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTV+MQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI 480

Query: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLIQG 540
           QPAGWAEW+GDFALKTLYYGEYSNTGPGSDVSKRVKW GYH+ITSPSEAQKFTV+SLIQG
Sbjct: 481 QPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTVNSLIQG 540

Query: 541 GEWLGPSGATFIPGL 555
           GEWLGPSGA+FIPGL
Sbjct: 541 GEWLGPSGASFIPGL 555

BLAST of CsGy6G033260 vs. ExPASy TrEMBL
Match: A0A1S3B018 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103484595 PE=3 SV=1)

HSP 1 Score: 1050 bits (2714), Expect = 0.0
Identity = 522/555 (94.05%), Postives = 539/555 (97.12%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLL LQPPKTS SKTI TILCFA VLCSAIF+TNKFIK NP ENDLPFL NICHK
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYENDLPFLHNICHK 60

Query: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPRGE 120
           AY+PSSCIEMAASEFPLSIIKTTNE  FLQSFL+KSMPKVI+TIERAKD+RQRINSPRGE
Sbjct: 61  AYNPSSCIEMAASEFPLSIIKTTNEEHFLQSFLKKSMPKVINTIERAKDLRQRINSPRGE 120

Query: 121 AALADCIELMEISNGRIMDSVLALKNRTNGSIENSHTWLSSVLTNHVTCWDEVESSLSRA 180
            AL DCIELMEISNGRIMDSVLALKNRT+G IE+SHTWLSSVLTNHVTCWDEVESSLSRA
Sbjct: 121 GALDDCIELMEISNGRIMDSVLALKNRTSGYIESSHTWLSSVLTNHVTCWDEVESSLSRA 180

Query: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240
           AAMDLGLEELIMRGRNSLGMLVSIWGL+IKNLGEL K+GNGYPSWLKKGDRRLLGVLGRE
Sbjct: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGNGYPSWLKKGDRRLLGVLGRE 240

Query: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300
           MEPNIVVAKDGSGNFKTV+EAVESVPDKSKNRIVIYVK+GTY+ENVEVGKKKKNVMIVGD
Sbjct: 241 MEPNIVVAKDGSGNFKTVQEAVESVPDKSKNRIVIYVKKGTYKENVEVGKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGP KHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIQQCDIVAS DLEPVKKSIKTYLGRPWKEYSRTV+MQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIQQCDIVASFDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI 480

Query: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLIQG 540
           QPAGWAEW+GDFALKTLYYGEYSNTGPGSDVSKRVKW GYH+ITSPSEAQKFTV+SLIQG
Sbjct: 481 QPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTVNSLIQG 540

Query: 541 GEWLGPSGATFIPGL 555
           GEWLGPSGA+FIPGL
Sbjct: 541 GEWLGPSGASFIPGL 555

BLAST of CsGy6G033260 vs. ExPASy TrEMBL
Match: A0A6J1KRW2 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496691 PE=3 SV=1)

HSP 1 Score: 886 bits (2289), Expect = 0.0
Identity = 448/557 (80.43%), Postives = 495/557 (88.87%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAAVLCSAIFITNKFIKPNPSENDL-PFLQNICH 60
           MATH+PLL  QPP+T+R KT  TIL F+ +L SAIFITNK I  NPSEND  PFL NIC+
Sbjct: 1   MATHEPLLP-QPPETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICY 60

Query: 61  KAYDPSSCIEMAASEFPL-SIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPR 120
           KAY+ SSCI+M ASEFPL    KTTN  +FLQSFLRKS  K+++TIE AKD+R+RIN+PR
Sbjct: 61  KAYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPR 120

Query: 121 GEAALADCIELMEISNGRIMDSVLALKNRTNGSIENSHTWLSSVLTNHVTCWDEVESSLS 180
           GEAALADC+ELMEIS  RI DS+LAL+ R  G+IEN+HTWLSSVLTNHV C DE+E+S+S
Sbjct: 121 GEAALADCVELMEISMDRITDSILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSIS 180

Query: 181 RAAAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLG 240
           +AAAMDLG+EELI+ GRNSLGMLVSIWG         +K+G GYPSWLKKGDRRLL V+G
Sbjct: 181 KAAAMDLGVEELIVIGRNSLGMLVSIWGSG-------KKEGMGYPSWLKKGDRRLLEVVG 240

Query: 241 REMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIV 300
           R M+ +IVVAKDGSGNFKTVKEAVE+VPDKSK+RIVIYVK+G Y+ENVEVGK KKNVM+V
Sbjct: 241 RGMKADIVVAKDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLV 300

Query: 301 GDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGAD 360
           GDGMDSTIIT SLNVVDGSTTFKSATVAAVGDGFIAQDIWF+NTA PEKHQAVALRVGAD
Sbjct: 301 GDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGAD 360

Query: 361 QSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQA 420
           QSVINRCRIDAYQDTLY H+NRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM+NQA
Sbjct: 361 QSVINRCRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQA 420

Query: 421 NMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGD 480
           NMVTAQGR DPNQNTGTSIQ+C+IVAS DLEPVKKSIKTYLGRPWKEYSRTV+MQS +GD
Sbjct: 421 NMVTAQGRTDPNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGD 480

Query: 481 LIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLI 540
           LI PAGWAEWNG+FALKTLYYGEYSN GPGS+VSKRVKW GYH+ITSP+ AQKFTVDSLI
Sbjct: 481 LIDPAGWAEWNGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLI 540

Query: 541 QGGEWLGPSGATFIPGL 555
           QG EWLG SG TFIPGL
Sbjct: 541 QGAEWLGSSGVTFIPGL 549

BLAST of CsGy6G033260 vs. ExPASy TrEMBL
Match: A0A6J1CN37 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111012508 PE=3 SV=1)

HSP 1 Score: 849 bits (2194), Expect = 1.05e-306
Identity = 441/561 (78.61%), Postives = 474/561 (84.49%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MAT+QPLL  QPPKT RSK    ILCFA +LCSA FI N  I PNPSE D  FL NIC K
Sbjct: 1   MATNQPLL--QPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICRK 60

Query: 61  AYDPSSCIEMAASEFPLSI-IKTTNEVDFLQSFLRKSMPKVISTIERAKDI--RQRINSP 120
           AY+P+SC+ MAASE P     K TN V FLQ FLRKS+ KV  TIERA D+   +RIN  
Sbjct: 61  AYNPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANDLILSRRINDA 120

Query: 121 RGEAALADCIELMEISNGRIMDSVLAL--KNRTNGSIENSHTWLSSVLTNHVTCWDEVES 180
           RGEAALADC+ELMEIS  RI DS+LAL   N+T+  IE++H WLSSVLTNHVTC DE+E 
Sbjct: 121 RGEAALADCLELMEISMDRITDSILALALNNKTSVPIEDAHAWLSSVLTNHVTCSDELEK 180

Query: 181 SLSRAAAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGN-GYPSWLKKGDRRLL 240
           SL    A +LGLEELIM  RNSL MLVSI   +     E E +G  G+P WLK GDR LL
Sbjct: 181 SLLGTTAAELGLEELIMTARNSLAMLVSISQPE----PEPEIEGQIGFPGWLKSGDRMLL 240

Query: 241 GVLGREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKN 300
           GV G++++ NIVVAKDGSGNFKTVKEAV SVPDKSKNR VIYVK+GTYEENVEV KKKKN
Sbjct: 241 GVTGQQIKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKN 300

Query: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALR 360
           VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWF+NTAGPEKHQAVALR
Sbjct: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALR 360

Query: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM 420
           VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIE RRPM
Sbjct: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPM 420

Query: 421 NNQANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQS 480
           +NQANMVTAQGRIDPNQNTGTSI +C IVASSDLEPVK+SIKTYLGRPWKEYSRTVIMQS
Sbjct: 421 SNQANMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQS 480

Query: 481 RIGDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTV 540
           RIGDLI P GWAEWNGDFALKTLYYGEY N GPGSDVSKRVKW GY +IT+P+EAQKFTV
Sbjct: 481 RIGDLIDPTGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTV 540

Query: 541 DSLIQGGEWLGPSGATFIPGL 555
           DSLIQGG+WLGP+G TFIPGL
Sbjct: 541 DSLIQGGDWLGPTGVTFIPGL 555

BLAST of CsGy6G033260 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 579.3 bits (1492), Expect = 3.2e-165
Identity = 309/558 (55.38%), Postives = 387/558 (69.35%), Query Frame = 0

Query: 3   THQPLLLLQPPKTSRSKTIPTILCFAAVLCSAIFITNKFIKPNPSENDLPFL--QNICHK 62
           ++QP  LL  PK+ + K +  +L F A+L S  F T + I  N + ND   L    ICH 
Sbjct: 4   SNQP--LLSKPKSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLLTTSQICHG 63

Query: 63  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPRGE 122
           A+D  SC +   SEF    +   N +D L  FL+ S+ ++ ST+    + R R N  R +
Sbjct: 64  AHDQDSC-QALLSEFTTLSLSKLNRLDLLHVFLKNSVWRLESTMTMVSEARIRSNGVRDK 123

Query: 123 AALADCIELMEISNGRIMDSVLALK--NRTNGSIENSHTWLSSVLTNHVTCWDEV-ESSL 182
           A  ADC E+M++S  R+M S+  L+  N    S  N HTWLSSVLTN++TC + + + S+
Sbjct: 124 AGFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYMTCLESISDVSV 183

Query: 183 SRAAAMDLGLEELIMRGRNSLGMLVSIWGL--DIKNLGELEKKGNGYPSWLKKGDRRLLG 242
           +    +   LE+L+ R R +L + VS+     D+K +       N +PSWL   DR+LL 
Sbjct: 184 NSKQIVKPQLEDLVSRARVALAIFVSVLPARDDLKMI-----ISNRFPSWLTALDRKLLE 243

Query: 243 VLGREME--PNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKK 302
              + ++   N+VVAKDG+G FKTV EAV + P+ S  R VIYVK+G Y+E +++GKKKK
Sbjct: 244 SSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYVIYVKKGVYKETIDIGKKKK 303

Query: 303 NVMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVAL 362
           N+M+VGDG D+TIITGSLNV+DGSTTF+SATVAA GDGF+AQDIWFQNTAGP KHQAVAL
Sbjct: 304 NLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQDIWFQNTAGPAKHQAVAL 363

Query: 363 RVGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRP 422
           RV ADQ+VINRCRIDAYQDTLYTH+ RQFYRDS ITGTVDFIFGN+AVV QNC I  R P
Sbjct: 364 RVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNSAVVFQNCDIVARNP 423

Query: 423 MNNQANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQ 482
              Q NM+TAQGR D NQNT  SIQ+C I ASSDL PVK S+KT+LGRPWK YSRTVIMQ
Sbjct: 424 GAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFLGRPWKLYSRTVIMQ 483

Query: 483 SRIGDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFT 542
           S I + I PAGW  W+G+FAL TLYYGEY+NTGPG+D SKRV W G+ +I    EA++FT
Sbjct: 484 SFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKRVNWKGFKVIKDSKEAEQFT 543

Query: 543 VDSLIQGGEWLGPSGATF 552
           V  LIQGG WL P+G TF
Sbjct: 544 VAKLIQGGLWLKPTGVTF 553

BLAST of CsGy6G033260 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 439.1 bits (1128), Expect = 5.2e-123
Identity = 256/536 (47.76%), Postives = 319/536 (59.51%), Query Frame = 0

Query: 65  SSCIEMAASEFPLSIIKTTNEVDFL--QSFLRKSMPKVISTIER-----AKDIRQRIN-S 124
           SSC      E  +S + T   V+    +  +  S+   I+ +E       K I++R   +
Sbjct: 61  SSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLT 120

Query: 125 PRGEAALADCIELMEISNGRIMDSVLAL-----KNRTNGSIENSHTWLSSVLTNHVTCWD 184
           PR + AL DC+E ++ +   + ++V  L     K        +  T +SS +TN  TC D
Sbjct: 121 PREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLD 180

Query: 185 --------------------EVESSLSRAAAMDLGLEELIMRGRNSLGMLVS-------- 244
                                VE   S A AM   + +  +        + S        
Sbjct: 181 GFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEE 240

Query: 245 ----IWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGREMEPNIVVAKDGSGNFKTVK 304
                  +DI   GEL+ +  G+P+WL  GDRRLL   G  ++ +  VA DGSG FKTV 
Sbjct: 241 NQETTVAVDIAGAGELDSE--GWPTWLSAGDRRLL--QGSGVKADATVAADGSGTFKTVA 300

Query: 305 EAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGDGMDSTIITGSLNVVDGSTT 364
            AV + P+ S  R VI++K G Y ENVEV KKKKN+M +GDG   TIITGS NVVDGSTT
Sbjct: 301 AAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTT 360

Query: 365 FKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQSVINRCRIDAYQDTLYTHSN 424
           F SATVAAVG+ F+A+DI FQNTAGP KHQAVALRVG+D S    C + AYQDTLY HSN
Sbjct: 361 FHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSN 420

Query: 425 RQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANMVTAQGRIDPNQNTGTSIQQ 484
           RQF+    I GTVDFIFGNAAVVLQ+C I  RRP + Q NMVTAQGR DPNQNTG  IQ+
Sbjct: 421 RQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQK 480

Query: 485 CDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLIQPAGWAEWNGDFALKTLYY 544
           C I A+SDL+ VK S  TYLGRPWKEYS+TVIMQS I D+I+P GW+EW G FAL TL Y
Sbjct: 481 CRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTY 540

Query: 545 GEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLIQGGEWLGPSGATFIPGL 556
            EYSNTG G+  + RVKW G+ +IT+ +EAQK+T    I GG WL  +G  F  GL
Sbjct: 541 REYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of CsGy6G033260 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 434.9 bits (1117), Expect = 9.9e-122
Identity = 243/545 (44.59%), Postives = 324/545 (59.45%), Query Frame = 0

Query: 30  VLCSAIF-----ITNKFIKPNPSENDLPFLQNICHKAYDPSSCIEMAASEFPLSIIKTTN 89
           V+C  IF     + +   +P  +      +   C K+  P+ CI+    +FP S+  T +
Sbjct: 46  VVCFGIFAGIRAVDSGKTEPKLTRKPTQAISRTCSKSLYPNLCID-TLLDFPGSL--TAD 105

Query: 90  EVDFLQSFLRKSMPKVISTIERAKDIRQRINSPRGEAALADCIELMEISNG---RIMDSV 149
           E + +      ++ K    +  +  I      PR  +A   C+EL++ S     R + SV
Sbjct: 106 ENELIHISFNATLQKFSKALYTSSTITYTQMPPRVRSAYDSCLELLDDSVDALTRALSSV 165

Query: 150 LALKNRTNGSIENSHTWLSSVLTNHVTC---WDEVESSLSRAAAMDLG-LEELIMRGRNS 209
           + +    + S  +  TWLSS +TNH TC   +DE+E          +G +++L     N 
Sbjct: 166 VVVSG--DESHSDVMTWLSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNC 225

Query: 210 L----GMLVSIWGLDIKNLGEL--EKKGNGYPSWLKKGDRRLLGVLGREMEPNIVVAKDG 269
           L    G +  + G+ + N  +L   ++    P+WLK+ DR LLG     ++ +I V+KDG
Sbjct: 226 LAIFAGKVKDLSGVPVVNNRKLLGTEETEELPNWLKREDRELLGTPTSAIQADITVSKDG 285

Query: 270 SGNFKTVKEAVESVPDKSKNRIVIYVKRGTY-EENVEVGKKKKNVMIVGDGMDSTIITGS 329
           SG FKT+ EA++  P+ S  R VIYVK G Y EEN++VG+KK N+M +GDG   T+ITG 
Sbjct: 286 SGTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGG 345

Query: 330 LNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQSVINRCRIDAY 389
            ++ D  TTF +AT AA G GFI +D+ F+N AGP KHQAVALRVG D +V+ RC I  Y
Sbjct: 346 KSIADDLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGY 405

Query: 390 QDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANMVTAQGRIDPN 449
           QD LY HSNRQF+R+  I GTVDFIFGNAAV+LQ+C I  R+PM  Q   +TAQ R DPN
Sbjct: 406 QDALYVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPN 465

Query: 450 QNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLIQPAGWAEWNG 509
           QNTG SI  C ++A+ DLE  K S  TYLGRPWK YSR V M S +GD I P GW EWNG
Sbjct: 466 QNTGISIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNG 525

Query: 510 DFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLIQGGEWLGPSGAT 556
            FAL +LYYGEY N G GS + +RVKW GYH+ITS  EA KFTV   I G  WL  +G +
Sbjct: 526 PFALDSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVS 585

BLAST of CsGy6G033260 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 424.5 bits (1090), Expect = 1.3e-118
Identity = 243/527 (46.11%), Postives = 309/527 (58.63%), Query Frame = 0

Query: 54  LQNICHKAYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQR 113
           L+++C     P  C    A+     +      ++   +   K++      +++    R+ 
Sbjct: 62  LKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKG 121

Query: 114 INSPRGEAALADCIELMEISNGRIMDSVLALKNRTNGSIENSH-----TWLSSVLTNHVT 173
           + +PR   AL DC+E ++ +   +  +V  L           H     T +SS +TN  T
Sbjct: 122 L-TPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGT 181

Query: 174 C-----WDEVESSLSRAAAMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKK----- 233
           C     +D+ +  + +A     G   +     N+L M+ ++   DI N    +K      
Sbjct: 182 CLDGFSYDDADRKVRKALLK--GQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTN 241

Query: 234 --------------GNGYPSWLKKGDRRLLGVLGREMEPNIVVAKDGSGNFKTVKEAVES 293
                          +G+P WL  GDRRLL   G  ++ +  VA DGSG+F TV  AV +
Sbjct: 242 NNNRKLKEVTGDLDSDGWPKWLSVGDRRLL--QGSTIKADATVADDGSGDFTTVAAAVAA 301

Query: 294 VPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGDGMDSTIITGSLNVVDGSTTFKSAT 353
            P+KS  R VI++K G Y ENVEV KKK N+M +GDG   TIITGS NVVDGSTTF SAT
Sbjct: 302 APEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSAT 361

Query: 354 VAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQSVINRCRIDAYQDTLYTHSNRQFYR 413
           VAAVG+ F+A+DI FQNTAGP KHQAVALRVG+D S   +C + AYQDTLY HSNRQF+ 
Sbjct: 362 VAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFV 421

Query: 414 DSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANMVTAQGRIDPNQNTGTSIQQCDIVA 473
              ITGTVDFIFGNAA VLQ+C I  RRP + Q NMVTAQGR DPNQNTG  IQ C I  
Sbjct: 422 KCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGG 481

Query: 474 SSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLIQPAGWAEWNGDFALKTLYYGEYSN 533
           +SDL  VK +  TYLGRPWKEYSRTVIMQS I D+I+P GW EW+G FAL TL Y EY N
Sbjct: 482 TSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLN 541

Query: 534 TGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLIQGGEWLGPSGATF 552
            G G+  + RVKW GY +ITS +EAQ FT    I GG WL  +G  F
Sbjct: 542 RGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

BLAST of CsGy6G033260 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 424.5 bits (1090), Expect = 1.3e-118
Identity = 233/500 (46.60%), Postives = 312/500 (62.40%), Query Frame = 0

Query: 58  CHKAYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSP 117
           C +  +P  C            IK  +E +FL+  ++  + + I     A  +  +    
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIK--SESEFLKISMKLVLDRAILAKTHAFTLGPKCRDT 93

Query: 118 RGEAALADCIELMEISNGRIMDSVLALKNRTNGSIENSHTWLSSVLTNHVTCWDEVESSL 177
           R +AA  DCI+L +++  +I ++   +      S  ++ TWLS+ LTN  TC        
Sbjct: 94  REKAAWEDCIKLYDLTVSKINET---MDPNVKCSKLDAQTWLSTALTNLDTC-------- 153

Query: 178 SRAAAMDLGLEELI--MRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLG 237
            RA  ++LG+ +++  +   N   +L +   ++         + +G+PSW+K GDR+LL 
Sbjct: 154 -RAGFLELGVTDIVLPLMSNNVSNLLCNTLAINKVPFNYTPPEKDGFPSWVKPGDRKLL- 213

Query: 238 VLGREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNV 297
                 + N VVAKDGSGNFKT+KEA+++       R VIYVK+G Y EN+E+  +KKNV
Sbjct: 214 -QSSTPKDNAVVAKDGSGNFKTIKEAIDAA--SGSGRFVIYVKQGVYSENLEI--RKKNV 273

Query: 298 MIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRV 357
           M+ GDG+  TIITGS +V  G+TTF SATVAAVGDGFIA+ I F+NTAG    QAVALR 
Sbjct: 274 MLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALRS 333

Query: 358 GADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMN 417
           G+D SV  +C  +AYQDTLY HSNRQFYRD  + GTVDFIFGNAA VLQNC I  RRP  
Sbjct: 334 GSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP-R 393

Query: 418 NQANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSR 477
           ++ N +TAQGR DPNQNTG  I    + A+SDL PV  S KTYLGRPW++YSRTV M++ 
Sbjct: 394 SKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTS 453

Query: 478 IGDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVD 537
           +  LI P GW EW+G+FALKTL+Y E+ NTGPG+  S RV W G+ ++ S SEA KFTV 
Sbjct: 454 LDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVG 511

Query: 538 SLIQGGEWLGPSGATFIPGL 556
           + + GG W+ PS   F  GL
Sbjct: 514 TFLAGGSWI-PSSVPFTSGL 511